PRIMER PICKING RESULTS FOR exon1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         43   18   55.22   55.56  6.00  1.00  9.00 TCACCCACGAGTGAAGAC
RIGHT PRIMER       279   18   56.69   61.11  3.00  1.00  8.00 CTCCTCAGCCTTGTCACC
SEQUENCE SIZE: 365
INCLUDED REGION SIZE: 365

PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
EXCLUDED REGIONS (start, len)*: 100,150

    0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA
                                                 >>>>>>>>>>>>>>>>>

   60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCA
      >                                       XXXXXXXXXXXXXXXXXXXX

  120 GTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGC
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  180 TGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAA
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  240 GAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCA
      XXXXXXXXXX            <<<<<<<<<<<<<<<<<<                    

  300 GGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGC
                                                                  

  360 GCCGG
           

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3'   rep seq

 1 LEFT PRIMER         40   18   55.24   50.00  8.00  8.00  8.00 GTTTCACCCACGAGTGAA
   RIGHT PRIMER       279   18   56.69   61.11  3.00  1.00  8.00 CTCCTCAGCCTTGTCACC
   PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER         39   18   54.75   50.00  6.00  6.00  9.00 AGTTTCACCCACGAGTGA
   RIGHT PRIMER       279   18   56.69   61.11  3.00  1.00  8.00 CTCCTCAGCCTTGTCACC
   PRODUCT SIZE: 241, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER         38   18   54.75   55.56  4.00  4.00  8.00 GAGTTTCACCCACGAGTG
   RIGHT PRIMER       279   18   56.69   61.11  3.00  1.00  8.00 CTCCTCAGCCTTGTCACC
   PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER         43   18   55.22   55.56  6.00  1.00  9.00 TCACCCACGAGTGAAGAC
   RIGHT PRIMER       280   18   56.87   61.11  3.00  3.00  8.00 GCTCCTCAGCCTTGTCAC
   PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     877     1     0    91     0     7     0     2   399     0     0     0     0     0   377
Right   1012    19     0    58     0    25     0     0   702     0     0     0     0     0   208
Pair Stats:
considered 23, unacceptable product size 17, ok 6
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR exon2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         39   20   54.89   35.00  4.00  4.00  9.00 CCCAAATCCCATTATTCATA
RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
SEQUENCE SIZE: 342
INCLUDED REGION SIZE: 342

PRODUCT SIZE: 229, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
EXCLUDED REGIONS (start, len)*: 100,145

    0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
                                             >>>>>>>>>>>>>>>>>>>> 

   60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
                                              XXXXXXXXXXXXXXXXXXXX

  120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
      XXXXX<<<<<<<<<<<<<<<<<<<<<<<                                

  300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
                                                

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3'   rep seq

 1 LEFT PRIMER         39   20   54.89   35.00  4.00  4.00  9.00 CCCAAATCCCATTATTCATA
   RIGHT PRIMER       268   24   54.78   29.17  6.00  6.00  9.00 ATGATGTCTACTTTCCAAATTACA
   PRODUCT SIZE: 230, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER         21   20   54.19   45.00  6.00  4.00  8.00 CTTAAGTTTGTAACAGGGCC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER         20   20   54.19   45.00  6.00  2.00  8.00 CCTTAAGTTTGTAACAGGGC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER         19   21   55.19   42.86  6.00  2.00  8.00 ACCTTAAGTTTGTAACAGGGC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     728     0     0    69     0     2     0   126     0     0     0     1    23     0   507
Right    827     0     0    72     0   314     0   236     0     0     0     0     0     0   205
Pair Stats:
considered 9, unacceptable product size 4, ok 5
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR exon2

Using mispriming library not_for_production.lib
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         39   20   54.89   35.00  4.00  4.00  9.00 CCCAAATCCCATTATTCATA
RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
SEQUENCE SIZE: 342
INCLUDED REGION SIZE: 342

PRODUCT SIZE: 229, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
EXCLUDED REGIONS (start, len)*: 100,145

    0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
                                             >>>>>>>>>>>>>>>>>>>> 

   60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
                                              XXXXXXXXXXXXXXXXXXXX

  120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
      XXXXX<<<<<<<<<<<<<<<<<<<<<<<                                

  300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
                                                

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3'   rep seq

 1 LEFT PRIMER         39   20   54.89   35.00  4.00  4.00  9.00 CCCAAATCCCATTATTCATA
   RIGHT PRIMER       268   24   54.78   29.17  6.00  6.00  9.00 ATGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 230, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER         21   20   54.19   45.00  6.00  4.00  8.00 CTTAAGTTTGTAACAGGGCC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER         20   20   54.19   45.00  6.00  2.00  8.00 CCTTAAGTTTGTAACAGGGC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER         19   21   55.19   42.86  6.00  2.00  8.00 ACCTTAAGTTTGTAACAGGGC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     728     0     0    69     0     2     0   126     0     0     0     1    23     0   507
Right    827     0     0    72     0   314     0   236     0     0     0     0     0     0   205
Intl    3079     0     0     0     0   316     0   517   178     0     0     0    23     0  2045
Pair Stats:
considered 9, unacceptable product size 4, ok 5
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR exon1_2

Using mispriming library not_for_production.lib
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         40   18   55.24   50.00  8.00  8.00  8.00 GTTTCACCCACGAGTGAA
RIGHT PRIMER       279   18   56.69   61.11  3.00  1.00  8.00 CTCCTCAGCCTTGTCACC
INTERNAL OLIGO     210   20   59.99   55.00  4.00  2.00       CTTCGAGTGGTCAAAGGAGC
SEQUENCE SIZE: 365
INCLUDED REGION SIZE: 365

PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
EXCLUDED REGIONS (start, len)*: 100,150

    0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA
                                              >>>>>>>>>>>>>>>>>>  

   60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCA
                                              XXXXXXXXXXXXXXXXXXXX

  120 GTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGC
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  180 TGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAA
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  240 GAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCA
      XXXXXXXXXX            <<<<<<<<<<<<<<<<<<                    

  300 GGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGC
                                                                  

  360 GCCGG
           

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3'   rep seq

 1 LEFT PRIMER         38   18   54.75   55.56  4.00  4.00  8.00 GAGTTTCACCCACGAGTG
   RIGHT PRIMER       279   18   56.69   61.11  3.00  1.00  8.00 CTCCTCAGCCTTGTCACC
   INTERNAL OLIGO     210   20   59.99   55.00  4.00  2.00       CTTCGAGTGGTCAAAGGAGC
   PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER         43   18   55.22   55.56  6.00  1.00  9.00 TCACCCACGAGTGAAGAC
   RIGHT PRIMER       280   18   56.87   61.11  3.00  3.00  8.00 GCTCCTCAGCCTTGTCAC
   INTERNAL OLIGO     210   20   59.99   55.00  4.00  2.00       CTTCGAGTGGTCAAAGGAGC
   PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER         40   18   55.24   50.00  8.00  8.00  8.00 GTTTCACCCACGAGTGAA
   RIGHT PRIMER       280   18   56.87   61.11  3.00  3.00  8.00 GCTCCTCAGCCTTGTCAC
   INTERNAL OLIGO     210   20   59.99   55.00  4.00  2.00       CTTCGAGTGGTCAAAGGAGC
   PRODUCT SIZE: 241, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER         39   18   54.75   50.00  6.00  6.00  9.00 AGTTTCACCCACGAGTGA
   RIGHT PRIMER       280   18   56.87   61.11  3.00  3.00  8.00 GCTCCTCAGCCTTGTCAC
   INTERNAL OLIGO     210   20   59.99   55.00  4.00  2.00       CTTCGAGTGGTCAAAGGAGC
   PRODUCT SIZE: 242, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 4.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     877     1     0    91     0     7     0     2   399     0     0     0     0     0   377
Right   1012    19     0    58     0    25     0     0   702     0     0     0     0     0   208
Intl    3237    27     0     0     0    32     0    23  1880     0     0     0     0     0  1275
Pair Stats:
considered 26, unacceptable product size 17, high mispriming library similarity 2, ok 7
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR exon2_2

Using mispriming library not_for_production.lib
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         23   19   55.24   47.37  6.00  6.00  8.00 TAAGTTTGTAACAGGGCCC
RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
SEQUENCE SIZE: 342
INCLUDED REGION SIZE: 342

PRODUCT SIZE: 245, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
EXCLUDED REGIONS (start, len)*: 100,145

    0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
                             >>>>>>>>>>>>>>>>>>>                  

   60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
                                              XXXXXXXXXXXXXXXXXXXX

  120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
      XXXXX<<<<<<<<<<<<<<<<<<<<<<<                                

  300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
                                                

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3'   rep seq

 1 LEFT PRIMER         23   19   55.24   47.37  6.00  6.00  8.00 TAAGTTTGTAACAGGGCCC
   RIGHT PRIMER       268   24   54.78   29.17  6.00  6.00  9.00 ATGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 246, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER         22   20   56.79   45.00  6.00  6.00  8.00 TTAAGTTTGTAACAGGGCCC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 246, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER         24   18   55.03   50.00  6.00  6.00  8.00 AAGTTTGTAACAGGGCCC
   RIGHT PRIMER       267   23   54.24   30.43  6.00  6.00  9.00 TGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 244, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER         22   20   56.79   45.00  6.00  6.00  8.00 TTAAGTTTGTAACAGGGCCC
   RIGHT PRIMER       268   24   54.78   29.17  6.00  6.00  9.00 ATGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 5 LEFT PRIMER         24   18   55.03   50.00  6.00  6.00  8.00 AAGTTTGTAACAGGGCCC
   RIGHT PRIMER       268   24   54.78   29.17  6.00  6.00  9.00 ATGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 245, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 6 LEFT PRIMER         23   19   55.24   47.37  6.00  6.00  8.00 TAAGTTTGTAACAGGGCCC
   RIGHT PRIMER       269   25   54.96   28.00  6.00  6.00  9.00 TATGATGTCTACTTTCCAAATTACA
   INTERNAL OLIGO     127   20   59.94   40.00  6.00  2.00       GTTTGGCATGATTTTGCCTT
   PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     728     0     0    69     0     2     0   126     0     0     0    14    23     0   494
Right    827     0     0    72     0   314     0   236     0     0     0    19     0     0   186
Intl    3079     0     0     0     0   316     0   517   178     0     0     0    23     0  2045
Pair Stats:
considered 167, unacceptable product size 101, tm diff too large 12, high mispriming library similarity 46, ok 8
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR exon2_3

Using mispriming library not_for_production.lib
No internal oligo mishyb library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 342
INCLUDED REGION SIZE: 342

EXCLUDED REGIONS (start, len)*: 100,145

    0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA
                                                                  

   60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA
                                              XXXXXXXXXXXXXXXXXXXX

  120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG
      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

  240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA
      XXXXX                                                       

  300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
                                                

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     728     0     0    69     0     2     0   126     0     0     0   124    23     0   384
Right    827     0     0    72     0   314     0   236     0     0     0   176     0     0    29
Intl    3079     0     0     0     0   316     0   517   178     0     0     0    23     0  2045
Pair Stats:
considered 12459, unacceptable product size 6872, tm diff too large 4075, high mispriming library similarity 1512, ok 0
primer3 release 2.0.0


