SEQUENCE_ID=00
PRIMER_MAX_POLY_X=0
PRIMER_INTERNAL_MAX_POLY_X=0
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=50-100
PRIMER_MIN_TM=0.0
PRIMER_MAX_TM=100.0
PRIMER_MIN_SIZE=25
PRIMER_MAX_SIZE=25
PRIMER_INTERNAL_MIN_SIZE=25
PRIMER_INTERNAL_MAX_SIZE=25
PRIMER_INTERNAL_OPT_SIZE=25
PRIMER_OPT_SIZE=25
PRIMER_MAX_GC=100
PRIMER_MIN_GC=0
PRIMER_MAX_SELF_END=100
PRIMER_MAX_SELF_ANY=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_INTERNAL_MAX_SELF_ANY=100
PRIMER_INTERNAL_MAX_SELF_END=100 
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_TM=30
PRIMER_INTERNAL_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=0
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=01
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=02
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_PAIR_EXPLAIN=considered 2, ok 2
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
PRIMER_PAIR_1_PENALTY=14.227051
PRIMER_LEFT_1_PENALTY=2.731673
PRIMER_RIGHT_1_PENALTY=11.495378
PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_1_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
PRIMER_LEFT_1=2,25
PRIMER_RIGHT_1=54,25
PRIMER_LEFT_1_TM=62.732
PRIMER_RIGHT_1_TM=71.495
PRIMER_LEFT_1_GC_PERCENT=36.000
PRIMER_RIGHT_1_GC_PERCENT=56.000
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=10.00
PRIMER_RIGHT_1_SELF_END=12.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=53
PRIMER_PAIR_1_PRODUCT_TM=66.4
=
SEQUENCE_ID=03
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=04
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=05
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=06
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=07
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=14.227051
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=54,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=12.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=53
PRIMER_PAIR_0_PRODUCT_TM=66.4
=
SEQUENCE_ID=08
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=14.227051
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=54,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=12.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=53
PRIMER_PAIR_0_PRODUCT_TM=66.4
=
SEQUENCE_ID=09
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, no internal oligo 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=10
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=11
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_WARNING=Specified left primer < PRIMER_MIN_SIZE
PRIMER_ERROR=Specified left primer not in sequence
=
SEQUENCE_ID=12
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_ERROR=Not specified to pick internal oligos but a specific internal oligo is provided
=
SEQUENCE_ID=13
SEQUENCE_TEMPLATE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=cacgtcatctcatcaaatttgattg
SEQUENCE_PRIMER_REVCOMP=gaattgtttggaaatgacagtgagt
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=cacgtcaTCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=14
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATctcatcaaattTGATTG
SEQUENCE_PRIMER_REVCOMP=gaattgtttggaAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=81,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
