P3_COMMENT=The SEQUENCE_PRIMER and SEQUENCE_PRIMER_REVCOMP are found at two positions in the template. The causes there to be 2 pairs to be considered, including one in which the left primer it to the right of the right primer.
SEQUENCE_TEMPLATE=AGGCTAGGCGAGCTGAAAAATCCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCTAGGCGAGCTGTTTTTTCCTACAGGCTAGGCGAGCTGAAAAATCCTAC
PRIMER_MAX_TEMPLATE_MISPRIMING=12
PRIMER_PICK_ANYWAY=1
PRIMER_PRODUCT_SIZE_RANGE=30-100
PRIMER_GC_CLAMP=0
PRIMER_FIRST_BASE_INDEX=1
PRIMER_MIN_SIZE=19
PRIMER_TASK=pick_pcr_primers
PRIMER_MIN_GC=18
PRIMER_MAX_NS_ACCEPTED=1
PRIMER_MIN_TM=50
PRIMER_EXPLAIN_FLAG=1
PRIMER_OPT_SIZE=20
PRIMER_NUM_RETURN=1
PRIMER_MAX_SIZE=27
PRIMER_TM_FORMULA=1
SEQUENCE_PRIMER=AGGCTAGGCGAGCTGAAAAATCCTAC
SEQUENCE_PRIMER_REVCOMP=GTAGGAAAAAACAGCTCGCCTAGCCT
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=./primer3_config/
PRIMER_SALT_CORRECTIONS=1
PRIMER_MAX_HAIRPIN_TH=47
PRIMER_MAX_SELF_ANY_TH=47
PRIMER_MAX_SELF_END_TH=47
PRIMER_PAIR_MAX_COMPL_ANY_TH=30
=
