SEQUENCE_ID=Check left only
SEQUENCE_PRIMER=gtcctcgaacagctcagtcc
PRIMER_TASK=check_primers
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
=
SEQUENCE_ID=Check internal only
SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa
PRIMER_TASK=check_primers
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_INTERNAL_0=90,20
PRIMER_INTERNAL_0_TM=60.187
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
=
SEQUENCE_ID=Check right only
SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt
PRIMER_TASK=check_primers
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_RIGHT_0=199,20
PRIMER_RIGHT_0_TM=60.006
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_RIGHT_0_END_STABILITY=7.0000
=
SEQUENCE_ID=Pick the same primers as before
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=250,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=60.187
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
=
SEQUENCE_ID=Check primers with sequence
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_NUM_RETURN=5
PRIMER_TASK=check_primers
SEQUENCE_PRIMER=gtcctcgaacagctcagtcc
SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa
SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_INTERNAL_0=250,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=60.187
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
=
SEQUENCE_ID=Check primers without sequence
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_NUM_RETURN=5
PRIMER_TASK=check_primers
SEQUENCE_PRIMER=gtcctcgaacagctcagtcc
SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa
SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_INTERNAL_0_PENALTY=0.187324
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=199,20
PRIMER_INTERNAL_0=90,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_INTERNAL_0_TM=60.187
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=200
PRIMER_PAIR_0_PRODUCT_TM=63.1
=
SEQUENCE_ID=Check primers without sequence and internal
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_NUM_RETURN=5
PRIMER_TASK=check_primers
SEQUENCE_PRIMER=gtcctcgaacagctcagtcc
SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=199,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=200
PRIMER_PAIR_0_PRODUCT_TM=61.0
=
SEQUENCE_ID=Pick three times the same primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=pick_primer_list
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.000037
PRIMER_INTERNAL_0_PENALTY=0.000037
PRIMER_LEFT_0_SEQUENCE=gacgtttgctttctgcttcc
PRIMER_RIGHT_0_SEQUENCE=ggaagcagaaagcaaacgtc
PRIMER_INTERNAL_0_SEQUENCE=gacgtttgctttctgcttcc
PRIMER_LEFT_0=1471,20
PRIMER_RIGHT_0=1490,20
PRIMER_INTERNAL_0=1471,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=60.000
PRIMER_INTERNAL_0_TM=60.000
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.8000
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_INTERNAL_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_INTERNAL_1_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_LEFT_1=849,20
PRIMER_RIGHT_1=868,20
PRIMER_INTERNAL_1=849,20
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.000
PRIMER_INTERNAL_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_LEFT_2_PENALTY=0.006089
PRIMER_RIGHT_2_PENALTY=0.006089
PRIMER_INTERNAL_2_PENALTY=0.006089
PRIMER_LEFT_2_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_INTERNAL_2_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_LEFT_2=296,20
PRIMER_RIGHT_2=315,20
PRIMER_INTERNAL_2=296,20
PRIMER_LEFT_2_TM=60.006
PRIMER_RIGHT_2_TM=60.006
PRIMER_INTERNAL_2_TM=60.006
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_LEFT_3_PENALTY=0.006937
PRIMER_RIGHT_3_PENALTY=0.006937
PRIMER_INTERNAL_3_PENALTY=0.006937
PRIMER_LEFT_3_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_3_SEQUENCE=ggactgagctgttcgaggac
PRIMER_INTERNAL_3_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_LEFT_3=198,20
PRIMER_RIGHT_3=217,20
PRIMER_INTERNAL_3=198,20
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_LEFT_4_PENALTY=0.014419
PRIMER_RIGHT_4_PENALTY=0.014419
PRIMER_INTERNAL_4_PENALTY=0.014419
PRIMER_LEFT_4_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_4_SEQUENCE=agggagctgtgtccagagaa
PRIMER_INTERNAL_4_SEQUENCE=ttctctggacacagctccct
PRIMER_LEFT_4=39,20
PRIMER_RIGHT_4=58,20
PRIMER_INTERNAL_4=39,20
PRIMER_LEFT_4_TM=59.986
PRIMER_RIGHT_4_TM=59.986
PRIMER_INTERNAL_4_TM=59.986
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
=
SEQUENCE_ID=Make a list of a small range
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=pick_primer_list
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=20
PRIMER_LEFT_NUM_RETURNED=6
PRIMER_RIGHT_NUM_RETURNED=17
PRIMER_INTERNAL_NUM_RETURNED=20
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=1.277576
PRIMER_RIGHT_0_PENALTY=0.557487
PRIMER_INTERNAL_0_PENALTY=0.300301
PRIMER_LEFT_0_SEQUENCE=gatttgatggacatggatgc
PRIMER_RIGHT_0_SEQUENCE=ggcatccatgtccatcaaat
PRIMER_INTERNAL_0_SEQUENCE=gacatggatgccatcaatga
PRIMER_LEFT_0=3,20
PRIMER_RIGHT_0=23,20
PRIMER_INTERNAL_0=12,20
PRIMER_LEFT_0_TM=58.722
PRIMER_RIGHT_0_TM=60.557
PRIMER_INTERNAL_0_TM=60.300
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=11.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=9.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_LEFT_1_PENALTY=1.436758
PRIMER_RIGHT_1_PENALTY=1.277576
PRIMER_INTERNAL_1_PENALTY=0.557487
PRIMER_LEFT_1_SEQUENCE=tggatttgatggacatggat
PRIMER_RIGHT_1_SEQUENCE=gcatccatgtccatcaaatc
PRIMER_INTERNAL_1_SEQUENCE=atttgatggacatggatgcc
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=22,20
PRIMER_INTERNAL_1=4,20
PRIMER_LEFT_1_TM=58.563
PRIMER_RIGHT_1_TM=58.722
PRIMER_INTERNAL_1_TM=60.557
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_LEFT_2_PENALTY=1.436758
PRIMER_RIGHT_2_PENALTY=1.283829
PRIMER_INTERNAL_2_PENALTY=1.277576
PRIMER_LEFT_2_SEQUENCE=atggatttgatggacatgga
PRIMER_RIGHT_2_SEQUENCE=ggcatccatgtccatcaaa
PRIMER_INTERNAL_2_SEQUENCE=gatttgatggacatggatgc
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=23,19
PRIMER_INTERNAL_2=3,20
PRIMER_LEFT_2_TM=58.563
PRIMER_RIGHT_2_TM=60.284
PRIMER_INTERNAL_2_TM=58.722
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=47.368
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_LEFT_3_PENALTY=2.074289
PRIMER_RIGHT_3_PENALTY=1.436758
PRIMER_INTERNAL_3_PENALTY=1.283829
PRIMER_LEFT_3_SEQUENCE=atggatttgatggacatggat
PRIMER_RIGHT_3_SEQUENCE=catccatgtccatcaaatcc
PRIMER_INTERNAL_3_SEQUENCE=tttgatggacatggatgcc
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=21,20
PRIMER_INTERNAL_3=5,19
PRIMER_LEFT_3_TM=58.926
PRIMER_RIGHT_3_TM=58.563
PRIMER_INTERNAL_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=47.368
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_LEFT_4_PENALTY=2.844552
PRIMER_RIGHT_4_PENALTY=1.436758
PRIMER_INTERNAL_4_PENALTY=1.331130
PRIMER_LEFT_4_SEQUENCE=tggatttgatggacatgga
PRIMER_RIGHT_4_SEQUENCE=atccatgtccatcaaatcca
PRIMER_INTERNAL_4_SEQUENCE=gatggacatggatgccatc
PRIMER_LEFT_4=1,19
PRIMER_RIGHT_4=20,20
PRIMER_INTERNAL_4=8,19
PRIMER_LEFT_4_TM=58.155
PRIMER_RIGHT_4_TM=58.563
PRIMER_INTERNAL_4_TM=59.669
PRIMER_LEFT_4_GC_PERCENT=42.105
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=52.632
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=10.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_LEFT_5_PENALTY=3.413493
PRIMER_RIGHT_5_PENALTY=1.436758
PRIMER_INTERNAL_5_PENALTY=1.436758
PRIMER_LEFT_5_SEQUENCE=ggatttgatggacatggatgc
PRIMER_RIGHT_5_SEQUENCE=tccatgtccatcaaatccat
PRIMER_INTERNAL_5_SEQUENCE=ggatttgatggacatggatg
PRIMER_LEFT_5=2,21
PRIMER_RIGHT_5=19,20
PRIMER_INTERNAL_5=2,20
PRIMER_LEFT_5_TM=62.413
PRIMER_RIGHT_5_TM=58.563
PRIMER_INTERNAL_5_TM=58.563
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_INTERNAL_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_INTERNAL_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_INTERNAL_5_SELF_END=4.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_RIGHT_6_PENALTY=1.742755
PRIMER_INTERNAL_6_PENALTY=1.436758
PRIMER_RIGHT_6_SEQUENCE=atggcatccatgtccatca
PRIMER_INTERNAL_6_SEQUENCE=tggatttgatggacatggat
PRIMER_RIGHT_6=25,19
PRIMER_INTERNAL_6=1,20
PRIMER_RIGHT_6_TM=60.743
PRIMER_INTERNAL_6_TM=58.563
PRIMER_RIGHT_6_GC_PERCENT=47.368
PRIMER_INTERNAL_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_INTERNAL_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_INTERNAL_6_SELF_END=2.00
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_RIGHT_7_PENALTY=2.074289
PRIMER_INTERNAL_7_PENALTY=1.436758
PRIMER_RIGHT_7_SEQUENCE=atccatgtccatcaaatccat
PRIMER_INTERNAL_7_SEQUENCE=atggatttgatggacatgga
PRIMER_RIGHT_7=20,21
PRIMER_INTERNAL_7=0,20
PRIMER_RIGHT_7_TM=58.926
PRIMER_INTERNAL_7_TM=58.563
PRIMER_RIGHT_7_GC_PERCENT=38.095
PRIMER_INTERNAL_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=2.00
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_RIGHT_8_PENALTY=2.134627
PRIMER_INTERNAL_8_PENALTY=1.742755
PRIMER_RIGHT_8_SEQUENCE=atggcatccatgtccatcaa
PRIMER_INTERNAL_8_SEQUENCE=atggacatggatgccatca
PRIMER_RIGHT_8=25,20
PRIMER_INTERNAL_8=9,19
PRIMER_RIGHT_8_TM=62.135
PRIMER_INTERNAL_8_TM=60.743
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_INTERNAL_8_GC_PERCENT=47.368
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_INTERNAL_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_INTERNAL_8_SELF_END=5.00
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_RIGHT_9_PENALTY=2.454729
PRIMER_INTERNAL_9_PENALTY=1.742755
PRIMER_RIGHT_9_SEQUENCE=tggcatccatgtccatca
PRIMER_INTERNAL_9_SEQUENCE=tgatggacatggatgccat
PRIMER_RIGHT_9=24,18
PRIMER_INTERNAL_9=7,19
PRIMER_RIGHT_9_TM=60.455
PRIMER_INTERNAL_9_TM=60.743
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_INTERNAL_9_GC_PERCENT=47.368
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_INTERNAL_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_INTERNAL_9_SELF_END=8.00
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_RIGHT_10_PENALTY=2.527737
PRIMER_INTERNAL_10_PENALTY=1.891046
PRIMER_RIGHT_10_SEQUENCE=catccatgtccatcaaatcca
PRIMER_INTERNAL_10_SEQUENCE=ggacatggatgccatcaat
PRIMER_RIGHT_10=21,21
PRIMER_INTERNAL_10=11,19
PRIMER_RIGHT_10_TM=61.528
PRIMER_INTERNAL_10_TM=59.109
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_INTERNAL_10_GC_PERCENT=47.368
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_INTERNAL_10_SELF_ANY=9.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_INTERNAL_10_SELF_END=9.00
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_RIGHT_11_PENALTY=2.844552
PRIMER_INTERNAL_11_PENALTY=2.074289
PRIMER_RIGHT_11_SEQUENCE=tccatgtccatcaaatcca
PRIMER_INTERNAL_11_SEQUENCE=atggatttgatggacatggat
PRIMER_RIGHT_11=19,19
PRIMER_INTERNAL_11=0,21
PRIMER_RIGHT_11_TM=58.155
PRIMER_INTERNAL_11_TM=58.926
PRIMER_RIGHT_11_GC_PERCENT=42.105
PRIMER_INTERNAL_11_GC_PERCENT=38.095
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_INTERNAL_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_INTERNAL_11_SELF_END=2.00
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_RIGHT_12_PENALTY=2.951135
PRIMER_INTERNAL_12_PENALTY=2.134627
PRIMER_RIGHT_12_SEQUENCE=tggcatccatgtccatcaa
PRIMER_INTERNAL_12_SEQUENCE=ggacatggatgccatcaatg
PRIMER_RIGHT_12=24,19
PRIMER_INTERNAL_12=11,20
PRIMER_RIGHT_12_TM=61.951
PRIMER_INTERNAL_12_TM=62.135
PRIMER_RIGHT_12_GC_PERCENT=47.368
PRIMER_INTERNAL_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_INTERNAL_12_SELF_ANY=11.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_INTERNAL_12_SELF_END=11.00
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_RIGHT_13_PENALTY=3.275586
PRIMER_INTERNAL_13_PENALTY=2.134627
PRIMER_RIGHT_13_SEQUENCE=ggcatccatgtccatcaa
PRIMER_INTERNAL_13_SEQUENCE=tggacatggatgccatcaat
PRIMER_RIGHT_13=23,18
PRIMER_INTERNAL_13=10,20
PRIMER_RIGHT_13_TM=58.724
PRIMER_INTERNAL_13_TM=62.135
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_INTERNAL_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_INTERNAL_13_SELF_ANY=9.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_INTERNAL_13_SELF_END=9.00
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_RIGHT_14_PENALTY=3.413493
PRIMER_INTERNAL_14_PENALTY=2.134627
PRIMER_RIGHT_14_SEQUENCE=ggcatccatgtccatcaaatc
PRIMER_INTERNAL_14_SEQUENCE=atggacatggatgccatcaa
PRIMER_RIGHT_14=23,21
PRIMER_INTERNAL_14=9,20
PRIMER_RIGHT_14_TM=62.413
PRIMER_INTERNAL_14_TM=62.135
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_INTERNAL_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_INTERNAL_14_SELF_ANY=8.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_INTERNAL_14_SELF_END=7.00
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_RIGHT_15_PENALTY=3.413493
PRIMER_INTERNAL_15_PENALTY=2.134627
PRIMER_RIGHT_15_SEQUENCE=gcatccatgtccatcaaatcc
PRIMER_INTERNAL_15_SEQUENCE=ttgatggacatggatgccat
PRIMER_RIGHT_15=22,21
PRIMER_INTERNAL_15=6,20
PRIMER_RIGHT_15_TM=62.413
PRIMER_INTERNAL_15_TM=62.135
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_INTERNAL_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_INTERNAL_15_SELF_ANY=8.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_INTERNAL_15_SELF_END=8.00
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_RIGHT_16_PENALTY=3.719699
PRIMER_INTERNAL_16_PENALTY=2.454729
PRIMER_RIGHT_16_SEQUENCE=catccatgtccatcaaatccat
PRIMER_INTERNAL_16_SEQUENCE=tggacatggatgccatca
PRIMER_RIGHT_16=21,22
PRIMER_INTERNAL_16=10,18
PRIMER_RIGHT_16_TM=61.720
PRIMER_INTERNAL_16_TM=60.455
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_INTERNAL_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_INTERNAL_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_INTERNAL_16_SELF_END=5.00
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_INTERNAL_17_PENALTY=2.454729
PRIMER_INTERNAL_17_SEQUENCE=tgatggacatggatgcca
PRIMER_INTERNAL_17=7,18
PRIMER_INTERNAL_17_TM=60.455
PRIMER_INTERNAL_17_GC_PERCENT=50.000
PRIMER_INTERNAL_17_SELF_ANY=6.00
PRIMER_INTERNAL_17_SELF_END=6.00
PRIMER_INTERNAL_18_PENALTY=2.527737
PRIMER_INTERNAL_18_SEQUENCE=tggatttgatggacatggatg
PRIMER_INTERNAL_18=1,21
PRIMER_INTERNAL_18_TM=61.528
PRIMER_INTERNAL_18_GC_PERCENT=42.857
PRIMER_INTERNAL_18_SELF_ANY=4.00
PRIMER_INTERNAL_18_SELF_END=4.00
PRIMER_INTERNAL_19_PENALTY=2.756256
PRIMER_INTERNAL_19_SEQUENCE=gatggacatggatgccatca
PRIMER_INTERNAL_19=8,20
PRIMER_INTERNAL_19_TM=62.756
PRIMER_INTERNAL_19_GC_PERCENT=50.000
PRIMER_INTERNAL_19_SELF_ANY=10.00
PRIMER_INTERNAL_19_SELF_END=6.00
=
SEQUENCE_ID=Make a list of a small range
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=pick_primer_list
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_NUM_RETURN=2
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.557487
PRIMER_RIGHT_0_SEQUENCE=ggcatccatgtccatcaaat
PRIMER_RIGHT_0=23,20
PRIMER_RIGHT_0_TM=60.557
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_RIGHT_1_PENALTY=1.277576
PRIMER_RIGHT_1_SEQUENCE=gcatccatgtccatcaaatc
PRIMER_RIGHT_1=22,20
PRIMER_RIGHT_1_TM=58.722
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_RIGHT_1_END_STABILITY=6.9000
=
SEQUENCE_ID=Check left only too small
SEQUENCE_PRIMER=gtcctcgaaca
PRIMER_TASK=check_primers
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_ANYWAY=1
PRIMER_WARNING=Specified left primer < PRIMER_MIN_SIZE
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=41.742994
PRIMER_LEFT_0_PROBLEMS= Temperature too low; Too short;
PRIMER_LEFT_0_SEQUENCE=gtcctcgaaca
PRIMER_LEFT_0=0,11
PRIMER_LEFT_0_TM=27.257
PRIMER_LEFT_0_GC_PERCENT=54.545
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
=
SEQUENCE_ID=Check left only too big
SEQUENCE_PRIMER=tgacctccacttctctggacacagctccctcaccaa
PRIMER_TASK=check_primers
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_ANYWAY=1
PRIMER_WARNING=Specified left primer > PRIMER_MAX_SIZE
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=36.812317
PRIMER_LEFT_0_PROBLEMS= Temperature too high; Too long;
PRIMER_LEFT_0_SEQUENCE=tgacctccacttctctggacacagctccctcaccaa
PRIMER_LEFT_0=0,36
PRIMER_LEFT_0_TM=80.812
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
=
SEQUENCE_ID=Pick sequencing primers no target
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=50
PRIMER_LEFT_NUM_RETURNED=4
PRIMER_RIGHT_NUM_RETURNED=4
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=1.134011
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_SEQUENCE=atgccatcaatgacctcca
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=19,19
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=59.866
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_LEFT_1_PENALTY=0.120245
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=acgcatgaagttggtgatga
PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_1=312,20
PRIMER_RIGHT_1=697,20
PRIMER_LEFT_1_TM=60.120
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.051345
PRIMER_LEFT_2_SEQUENCE=catcttcggataaccgcatt
PRIMER_RIGHT_2_SEQUENCE=gaaaaccagcctccaatgaa
PRIMER_LEFT_2=801,20
PRIMER_RIGHT_2=1203,20
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=60.051
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_LEFT_3_PENALTY=0.230279
PRIMER_RIGHT_3_PENALTY=2.744773
PRIMER_LEFT_3_SEQUENCE=ggggagtggttaggtgaggt
PRIMER_RIGHT_3_SEQUENCE=gaagaagaacaagtccaaaggg
PRIMER_LEFT_3=1317,20
PRIMER_RIGHT_3=1510,22
PRIMER_LEFT_3_TM=60.230
PRIMER_RIGHT_3_TM=59.255
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
=
SEQUENCE_ID=Pick sequencing primers small target
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_TARGET=450,37
PRIMER_NUM_RETURN=50
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.640220
PRIMER_RIGHT_0_PENALTY=0.501869
PRIMER_LEFT_0_SEQUENCE=aaccacaacaacaacaacgg
PRIMER_RIGHT_0_SEQUENCE=aagtggccgaatttgacgta
PRIMER_LEFT_0=284,20
PRIMER_RIGHT_0=670,20
PRIMER_LEFT_0_TM=59.360
PRIMER_RIGHT_0_TM=60.502
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=7.1000
=
SEQUENCE_ID=Pick sequencing primers left only
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_TARGET=150,800
PRIMER_NUM_RETURN=50
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=1.134011
PRIMER_LEFT_0_SEQUENCE=atgccatcaatgacctcca
PRIMER_LEFT_0=19,19
PRIMER_LEFT_0_TM=59.866
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_LEFT_1_PENALTY=0.405717
PRIMER_LEFT_1_SEQUENCE=agacttgccgctcatttcac
PRIMER_LEFT_1=477,20
PRIMER_LEFT_1_TM=60.406
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
=
SEQUENCE_ID=Pick sequencing primers right only
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=0
SEQUENCE_TARGET=150,800
PRIMER_NUM_RETURN=50
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.032351
PRIMER_RIGHT_0_SEQUENCE=tggaaagcagcaagtgtgtc
PRIMER_RIGHT_0=628,20
PRIMER_RIGHT_0_TM=60.032
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_RIGHT_1_PENALTY=0.660780
PRIMER_RIGHT_1_SEQUENCE=cttcaaccaacgtcacaagc
PRIMER_RIGHT_1=1107,20
PRIMER_RIGHT_1_TM=59.339
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_RIGHT_1_END_STABILITY=8.5000
=
SEQUENCE_ID=Pick sequencing primers three target
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_TARGET=350,37 450,100 850,500 1200,100
PRIMER_NUM_RETURN=50
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.369208
PRIMER_RIGHT_0_PENALTY=0.298942
PRIMER_LEFT_0_SEQUENCE=ccatcatcgaggacagaaca
PRIMER_RIGHT_0_SEQUENCE=ttgttgtggtgatggtggtt
PRIMER_LEFT_0=175,20
PRIMER_RIGHT_0=562,20
PRIMER_LEFT_0_TM=59.631
PRIMER_RIGHT_0_TM=59.701
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_LEFT_1_PENALTY=0.006089
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_1=296,20
PRIMER_RIGHT_1=697,20
PRIMER_LEFT_1_TM=60.006
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_LEFT_2_PENALTY=0.262854
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=acttcacagccaatcaagcc
PRIMER_RIGHT_2_SEQUENCE=cggtgcacggtaactaaggt
PRIMER_LEFT_2=667,20
PRIMER_RIGHT_2=1040,20
PRIMER_LEFT_2_TM=60.263
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_LEFT_3_PENALTY=0.061961
PRIMER_RIGHT_3_PENALTY=2.744773
PRIMER_LEFT_3_SEQUENCE=agcggtttttgattcattgg
PRIMER_RIGHT_3_SEQUENCE=gaagaagaacaagtccaaaggg
PRIMER_LEFT_3=1172,20
PRIMER_RIGHT_3=1510,22
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=59.255
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_LEFT_4_PENALTY=0.248900
PRIMER_RIGHT_4_PENALTY=0.040772
PRIMER_LEFT_4_SEQUENCE=cggaatcagttgcttcgttt
PRIMER_RIGHT_4_SEQUENCE=cacccacctcttcaacccta
PRIMER_LEFT_4=1039,20
PRIMER_RIGHT_4=1434,20
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.959
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
=
SEQUENCE_ID=Pick fixed left primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_LEFT_START=15
SEQUENCE_FORCE_LEFT_END=34
PRIMER_NUM_RETURN=3
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=0.172685
PRIMER_LEFT_0_PENALTY=0.165748
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=60.166
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=203
PRIMER_PAIR_0_PRODUCT_TM=79.0
PRIMER_PAIR_1_PENALTY=0.196791
PRIMER_LEFT_1_PENALTY=0.165748
PRIMER_RIGHT_1_PENALTY=0.031043
PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt
PRIMER_LEFT_1=15,20
PRIMER_RIGHT_1=125,20
PRIMER_LEFT_1_TM=60.166
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=111
PRIMER_PAIR_1_PRODUCT_TM=76.7
PRIMER_PAIR_2_PENALTY=0.277562
PRIMER_LEFT_2_PENALTY=0.165748
PRIMER_RIGHT_2_PENALTY=0.111814
PRIMER_LEFT_2_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_2_SEQUENCE=agttgacgatgggagaccag
PRIMER_LEFT_2=15,20
PRIMER_RIGHT_2=126,20
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=60.112
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
=
SEQUENCE_ID=Pick fixed right primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_RIGHT_START=697
SEQUENCE_FORCE_RIGHT_END=678
PRIMER_NUM_RETURN=3
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=0.233855
PRIMER_LEFT_0_PENALTY=0.048588
PRIMER_RIGHT_0_PENALTY=0.185267
PRIMER_LEFT_0_SEQUENCE=cgacaaccaaaacgacacac
PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_0=596,20
PRIMER_RIGHT_0=697,20
PRIMER_LEFT_0_TM=60.049
PRIMER_RIGHT_0_TM=60.185
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=102
PRIMER_PAIR_0_PRODUCT_TM=73.3
PRIMER_PAIR_1_PENALTY=0.233855
PRIMER_LEFT_1_PENALTY=0.048588
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=acgacaaccaaaacgacaca
PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_1=595,20
PRIMER_RIGHT_1=697,20
PRIMER_LEFT_1_TM=60.049
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=103
PRIMER_PAIR_1_PRODUCT_TM=73.2
PRIMER_PAIR_2_PENALTY=0.233855
PRIMER_LEFT_2_PENALTY=0.048588
PRIMER_RIGHT_2_PENALTY=0.185267
PRIMER_LEFT_2_SEQUENCE=cacgacaaccaaaacgacac
PRIMER_RIGHT_2_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_2=594,20
PRIMER_RIGHT_2=697,20
PRIMER_LEFT_2_TM=60.049
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=104
PRIMER_PAIR_2_PRODUCT_TM=73.5
=
SEQUENCE_ID=Pick start fixed left primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_LEFT_START=15
PRIMER_NUM_RETURN=3
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=0.172685
PRIMER_LEFT_0_PENALTY=0.165748
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=60.166
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=203
PRIMER_PAIR_0_PRODUCT_TM=79.0
PRIMER_PAIR_1_PENALTY=0.196791
PRIMER_LEFT_1_PENALTY=0.165748
PRIMER_RIGHT_1_PENALTY=0.031043
PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt
PRIMER_LEFT_1=15,20
PRIMER_RIGHT_1=125,20
PRIMER_LEFT_1_TM=60.166
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=111
PRIMER_PAIR_1_PRODUCT_TM=76.7
PRIMER_PAIR_2_PENALTY=0.277562
PRIMER_LEFT_2_PENALTY=0.165748
PRIMER_RIGHT_2_PENALTY=0.111814
PRIMER_LEFT_2_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_2_SEQUENCE=agttgacgatgggagaccag
PRIMER_LEFT_2=15,20
PRIMER_RIGHT_2=126,20
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=60.112
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
=
SEQUENCE_ID=Pick start fixed right primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_RIGHT_START=697
PRIMER_NUM_RETURN=3
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=0.233855
PRIMER_LEFT_0_PENALTY=0.048588
PRIMER_RIGHT_0_PENALTY=0.185267
PRIMER_LEFT_0_SEQUENCE=cgacaaccaaaacgacacac
PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_0=596,20
PRIMER_RIGHT_0=697,20
PRIMER_LEFT_0_TM=60.049
PRIMER_RIGHT_0_TM=60.185
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=102
PRIMER_PAIR_0_PRODUCT_TM=73.3
PRIMER_PAIR_1_PENALTY=0.233855
PRIMER_LEFT_1_PENALTY=0.048588
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=acgacaaccaaaacgacaca
PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_1=595,20
PRIMER_RIGHT_1=697,20
PRIMER_LEFT_1_TM=60.049
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=103
PRIMER_PAIR_1_PRODUCT_TM=73.2
PRIMER_PAIR_2_PENALTY=0.233855
PRIMER_LEFT_2_PENALTY=0.048588
PRIMER_RIGHT_2_PENALTY=0.185267
PRIMER_LEFT_2_SEQUENCE=cacgacaaccaaaacgacac
PRIMER_RIGHT_2_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_2=594,20
PRIMER_RIGHT_2=697,20
PRIMER_LEFT_2_TM=60.049
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=104
PRIMER_PAIR_2_PRODUCT_TM=73.5
=
SEQUENCE_ID=Pick end fixed left primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_LEFT_END=34
PRIMER_NUM_RETURN=2
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=0.172685
PRIMER_LEFT_0_PENALTY=0.165748
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=60.166
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=203
PRIMER_PAIR_0_PRODUCT_TM=79.0
PRIMER_PAIR_1_PENALTY=0.196791
PRIMER_LEFT_1_PENALTY=0.165748
PRIMER_RIGHT_1_PENALTY=0.031043
PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt
PRIMER_LEFT_1=15,20
PRIMER_RIGHT_1=125,20
PRIMER_LEFT_1_TM=60.166
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=111
PRIMER_PAIR_1_PRODUCT_TM=76.7
=
SEQUENCE_ID=Pick end fixed right primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_RIGHT_END=678
PRIMER_NUM_RETURN=2
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=0.233855
PRIMER_LEFT_0_PENALTY=0.048588
PRIMER_RIGHT_0_PENALTY=0.185267
PRIMER_LEFT_0_SEQUENCE=cgacaaccaaaacgacacac
PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_0=596,20
PRIMER_RIGHT_0=697,20
PRIMER_LEFT_0_TM=60.049
PRIMER_RIGHT_0_TM=60.185
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=102
PRIMER_PAIR_0_PRODUCT_TM=73.3
PRIMER_PAIR_1_PENALTY=0.233855
PRIMER_LEFT_1_PENALTY=0.048588
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=acgacaaccaaaacgacaca
PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_1=595,20
PRIMER_RIGHT_1=697,20
PRIMER_LEFT_1_TM=60.049
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=103
PRIMER_PAIR_1_PRODUCT_TM=73.2
=
SEQUENCE_ID=Pick cloning primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_cloning_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_INCLUDED_REGION=15,203
PRIMER_NUM_RETURN=2
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=0.172685
PRIMER_LEFT_0_PENALTY=0.165748
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=60.166
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=203
PRIMER_PAIR_0_PRODUCT_TM=79.0
PRIMER_PAIR_1_PENALTY=1.897984
PRIMER_LEFT_1_PENALTY=1.891046
PRIMER_RIGHT_1_PENALTY=0.006937
PRIMER_LEFT_1_PROBLEMS= Similarity to self too high;
PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacc
PRIMER_RIGHT_1_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_1=15,19
PRIMER_RIGHT_1=217,20
PRIMER_LEFT_1_TM=59.109
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=47.368
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=203
PRIMER_PAIR_1_PRODUCT_TM=79.0
=
SEQUENCE_ID=Pick cloning primers with only right primer
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_cloning_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=0
SEQUENCE_INCLUDED_REGION=15,203
PRIMER_NUM_RETURN=2
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_RIGHT_0=217,20
PRIMER_RIGHT_0_TM=59.993
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_RIGHT_1_PENALTY=1.938271
PRIMER_RIGHT_1_SEQUENCE=ggactgagctgttcgagga
PRIMER_RIGHT_1=217,19
PRIMER_RIGHT_1_TM=59.062
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_RIGHT_1_END_STABILITY=7.9000
=
SEQUENCE_ID=Pick discriminative primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_discriminative_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_TARGET=35,163
PRIMER_NUM_RETURN=2
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=0.172685
PRIMER_LEFT_0_PENALTY=0.165748
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=60.166
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=203
PRIMER_PAIR_0_PRODUCT_TM=79.0
PRIMER_PAIR_1_PENALTY=1.140948
PRIMER_LEFT_1_PENALTY=1.134011
PRIMER_RIGHT_1_PENALTY=0.006937
PRIMER_LEFT_1_PROBLEMS= Similary to 3' end of self too high;
PRIMER_LEFT_1_SEQUENCE=tggatgccatcaatgacct
PRIMER_RIGHT_1_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_1=16,19
PRIMER_RIGHT_1=217,20
PRIMER_LEFT_1_TM=59.866
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=47.368
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=202
PRIMER_PAIR_1_PRODUCT_TM=79.1
=
SEQUENCE_ID=Pick start fixed left primer unique
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_FORCE_LEFT_START=15
PRIMER_NUM_RETURN=3
PRIMER_MIN_THREE_PRIME_DISTANCE=0
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=0.172685
PRIMER_LEFT_0_PENALTY=0.165748
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct
PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=217,20
PRIMER_LEFT_0_TM=60.166
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=203
PRIMER_PAIR_0_PRODUCT_TM=79.0
PRIMER_PAIR_1_PENALTY=1.922089
PRIMER_LEFT_1_PENALTY=1.891046
PRIMER_RIGHT_1_PENALTY=0.031043
PRIMER_LEFT_1_PROBLEMS= Similarity to self too high;
PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacc
PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt
PRIMER_LEFT_1=15,19
PRIMER_RIGHT_1=125,20
PRIMER_LEFT_1_TM=59.109
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=47.368
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=111
PRIMER_PAIR_1_PRODUCT_TM=76.7
PRIMER_PAIR_2_PENALTY=3.159700
PRIMER_LEFT_2_PENALTY=3.047886
PRIMER_RIGHT_2_PENALTY=0.111814
PRIMER_LEFT_2_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high;
PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacctc
PRIMER_RIGHT_2_SEQUENCE=agttgacgatgggagaccag
PRIMER_LEFT_2=15,21
PRIMER_RIGHT_2=126,20
PRIMER_LEFT_2_TM=62.048
PRIMER_RIGHT_2_TM=60.112
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
=
SEQUENCE_ID=Pick a left primer and an internal oligo
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_INTERNAL_0_PENALTY=0.000037
PRIMER_LEFT_0_SEQUENCE=gacgtttgctttctgcttcc
PRIMER_INTERNAL_0_SEQUENCE=gacgtttgctttctgcttcc
PRIMER_LEFT_0=1471,20
PRIMER_INTERNAL_0=1471,20
PRIMER_LEFT_0_TM=60.000
PRIMER_INTERNAL_0_TM=60.000
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
=
SEQUENCE_ID=Pick a right primer and an internal oligo
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_LOWERCASE_MASKING=0
PRIMER_TASK=generic
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_NUM_RETURN=1
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.000037
PRIMER_INTERNAL_0_PENALTY=0.000037
PRIMER_RIGHT_0_SEQUENCE=ggaagcagaaagcaaacgtc
PRIMER_INTERNAL_0_SEQUENCE=gacgtttgctttctgcttcc
PRIMER_RIGHT_0=1490,20
PRIMER_INTERNAL_0=1471,20
PRIMER_RIGHT_0_TM=60.000
PRIMER_INTERNAL_0_TM=60.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_RIGHT_0_END_STABILITY=7.8000
=
SEQUENCE_ID=Test behavior of SEQUENCE_PRIMER_PAIR_OK_REGION_LIST with PRIMER_TASK=pick_primer_list
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
PRIMER_TASK=pick_primer_list
PRIMER_NUM_RETURN=15
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=40-100
PRIMER_MIN_GC=0
PRIMER_MIN_TM=0
PRIMER_PICK_ANYWAY=0
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_POLY_X=100
PRIMER_MAX_SELF_ANY=100
PRIMER_MAX_SELF_END=100
PRIMER_FIRST_BASE_INDEX=1
PRIMER_MIN_SIZE=16
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17
PRIMER_LEFT_EXPLAIN=considered 954, not in any ok left region 939, ok 15
PRIMER_RIGHT_EXPLAIN=considered 954, not in any ok right region 951, ok 3
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=15
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=17.049867
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,17
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=45.950
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=29.412
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_LEFT_1_PENALTY=11.546670
PRIMER_RIGHT_1_PENALTY=20.075362
PRIMER_LEFT_1_SEQUENCE=ttgattcaatgaatctcaa
PRIMER_RIGHT_1_SEQUENCE=aaaaaaacacaatccA
PRIMER_LEFT_1=2,19
PRIMER_RIGHT_1=65,16
PRIMER_LEFT_1_TM=49.453
PRIMER_RIGHT_1_TM=43.925
PRIMER_LEFT_1_GC_PERCENT=26.316
PRIMER_RIGHT_1_GC_PERCENT=25.000
PRIMER_LEFT_1_SELF_ANY=11.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_LEFT_2_PENALTY=12.075654
PRIMER_RIGHT_2_PENALTY=20.694887
PRIMER_LEFT_2_SEQUENCE=cttgattcaatgaatctca
PRIMER_RIGHT_2_SEQUENCE=aaaaaacacaatccAg
PRIMER_LEFT_2=1,19
PRIMER_RIGHT_2=64,16
PRIMER_LEFT_2_TM=48.924
PRIMER_RIGHT_2_TM=43.305
PRIMER_LEFT_2_GC_PERCENT=31.579
PRIMER_RIGHT_2_GC_PERCENT=31.250
PRIMER_LEFT_2_SELF_ANY=11.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_LEFT_3_PENALTY=14.913528
PRIMER_LEFT_3_SEQUENCE=tgattcaatgaatctcaa
PRIMER_LEFT_3=3,18
PRIMER_LEFT_3_TM=47.086
PRIMER_LEFT_3_GC_PERCENT=27.778
PRIMER_LEFT_3_SELF_ANY=11.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_LEFT_4_PENALTY=14.913528
PRIMER_LEFT_4_SEQUENCE=ttgattcaatgaatctca
PRIMER_LEFT_4=2,18
PRIMER_LEFT_4_TM=47.086
PRIMER_LEFT_4_GC_PERCENT=27.778
PRIMER_LEFT_4_SELF_ANY=11.00
PRIMER_LEFT_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_LEFT_5_PENALTY=16.846122
PRIMER_LEFT_5_SEQUENCE=cttgattcaatgaatctc
PRIMER_LEFT_5=1,18
PRIMER_LEFT_5_TM=45.154
PRIMER_LEFT_5_GC_PERCENT=33.333
PRIMER_LEFT_5_SELF_ANY=11.00
PRIMER_LEFT_5_SELF_END=7.00
PRIMER_LEFT_5_END_STABILITY=6.3000
PRIMER_LEFT_6_PENALTY=18.624075
PRIMER_LEFT_6_SEQUENCE=tgattcaatgaatctca
PRIMER_LEFT_6=3,17
PRIMER_LEFT_6_TM=44.376
PRIMER_LEFT_6_GC_PERCENT=29.412
PRIMER_LEFT_6_SELF_ANY=11.00
PRIMER_LEFT_6_SELF_END=5.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_LEFT_7_PENALTY=19.982933
PRIMER_LEFT_7_SEQUENCE=gattcaatgaatctcaa
PRIMER_LEFT_7=4,17
PRIMER_LEFT_7_TM=43.017
PRIMER_LEFT_7_GC_PERCENT=29.412
PRIMER_LEFT_7_SELF_ANY=11.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_LEFT_8_PENALTY=19.982933
PRIMER_LEFT_8_SEQUENCE=ttgattcaatgaatctc
PRIMER_LEFT_8=2,17
PRIMER_LEFT_8_TM=43.017
PRIMER_LEFT_8_GC_PERCENT=29.412
PRIMER_LEFT_8_SELF_ANY=11.00
PRIMER_LEFT_8_SELF_END=7.00
PRIMER_LEFT_8_END_STABILITY=6.3000
PRIMER_LEFT_9_PENALTY=20.744280
PRIMER_LEFT_9_SEQUENCE=cttgattcaatgaatct
PRIMER_LEFT_9=1,17
PRIMER_LEFT_9_TM=42.256
PRIMER_LEFT_9_GC_PERCENT=29.412
PRIMER_LEFT_9_SELF_ANY=11.00
PRIMER_LEFT_9_SELF_END=9.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_LEFT_10_PENALTY=24.111010
PRIMER_LEFT_10_SEQUENCE=gattcaatgaatctca
PRIMER_LEFT_10=4,16
PRIMER_LEFT_10_TM=39.889
PRIMER_LEFT_10_GC_PERCENT=31.250
PRIMER_LEFT_10_SELF_ANY=11.00
PRIMER_LEFT_10_SELF_END=4.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_LEFT_11_PENALTY=24.111010
PRIMER_LEFT_11_SEQUENCE=tgattcaatgaatctc
PRIMER_LEFT_11=3,16
PRIMER_LEFT_11_TM=39.889
PRIMER_LEFT_11_GC_PERCENT=31.250
PRIMER_LEFT_11_SELF_ANY=11.00
PRIMER_LEFT_11_SELF_END=7.00
PRIMER_LEFT_11_END_STABILITY=6.3000
PRIMER_LEFT_12_PENALTY=24.152694
PRIMER_LEFT_12_SEQUENCE=attcaatgaatctcaa
PRIMER_LEFT_12=5,16
PRIMER_LEFT_12_TM=39.847
PRIMER_LEFT_12_GC_PERCENT=25.000
PRIMER_LEFT_12_SELF_ANY=9.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_LEFT_13_PENALTY=24.152694
PRIMER_LEFT_13_SEQUENCE=ttgattcaatgaatct
PRIMER_LEFT_13=2,16
PRIMER_LEFT_13_TM=39.847
PRIMER_LEFT_13_GC_PERCENT=25.000
PRIMER_LEFT_13_SELF_ANY=11.00
PRIMER_LEFT_13_SELF_END=9.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_LEFT_14_PENALTY=24.152694
PRIMER_LEFT_14_SEQUENCE=cttgattcaatgaatc
PRIMER_LEFT_14=1,16
PRIMER_LEFT_14_TM=39.847
PRIMER_LEFT_14_GC_PERCENT=31.250
PRIMER_LEFT_14_SELF_ANY=11.00
PRIMER_LEFT_14_SELF_END=11.00
PRIMER_LEFT_14_END_STABILITY=6.6000
=
SEQUENCE_ID=rev_comp_primers
PRIMER_TASK=check_primers
SEQUENCE_PRIMER=CACCCCCATCAAACTCTTACTCA
SEQUENCE_PRIMER_REVCOMP=TGAGTAAGAGTTTGATGGGGGTG
PRIMER_ERROR=Specified left and right primer are reverse complementary
=
SEQUENCE_ID=identical_primers
PRIMER_TASK=check_primers
SEQUENCE_PRIMER=CACCCCCATCAAACTCTTACTCA
SEQUENCE_PRIMER_REVCOMP=CACCCCCATCAAACTCTTACTCA
PRIMER_ERROR=Specified left and right primer are identical
=
