PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_POLY_X=4
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_INTERNAL_DNA_CONC=200
PRIMER_INTERNAL_SALT_MONOVALENT=1
PRIMER_INTERNAL_SALT_DIVALENT=3
PRIMER_INTERNAL_MAX_SIZE=32
PRIMER_INTERNAL_OPT_SIZE=30
PRIMER_INTERNAL_MIN_SIZE=28
SEQUENCE_ID=IO_sec_str_thal_args_non_def_non_primer_args_1
SEQUENCE_TEMPLATE=CACAGTTATGCACAGAGCTGCAAACAACTATACATGATATAATATTAGAATGTGTGTACTGCAAGCAACAGTTACTGCGACGTGAGGTATATGACTTTGCTTTTCGGGATTTATGCATAGTATATAGAGATGGGAATCCATATGCTGTATGTGATAAATGTTTAAAGTTTTATTCTAAAATTAGTGAGTATAGACATTATTGTTATAGTGTGTATGGAACAACATTAGAACAGCAATACAACAAACCGTTGTGTGATTTGTTAATTAGGTGTATTAACTGTCAAAAGCCACTGTGTCCTGAAGAAAAGCAAAGACATCTGGACAAAAAGCAAAGATTCCATAATATAAGGGGTCGGTGGACCGGTCGATGTATGTCTTGTTGCAGATCATCAAGAACACGTAGAGAAACCCAGCTGTAATCATGCATGGAGATACACCTACATTGCATGAATATATGTTAGATTTGCAACCAGAGACAACTGATCTCTACTGTTATGAGCAATTAAATGACAGCTCAGAGGAGGAGGATGAAATAGATGGTCCAGCTGGACAAGCAGAACCGGACAGAGCCCATTACAATATTGTAACCTTTTGTTGCAAGTGTGACTCTACGCTTCGGTTGTGCGTACAAAGCACACACGTAGACATTCGTACTTTGGAAGACCTGTTAATGGGCACACTAGGAATTGTGTGCCCCATCTGTTCTCAGAAACCATAATCTACCATGGCTGATCCTGCAGGTACCAATGGGGAAGAGGGTACGGGATGTAATGGATGGTTTTATGTAGAGGCTGTAGTGGAAAAAAAAACAGGGGATGCTATATCAGATGACGAGAACGAAAATGACAGTGATACAGGTGAAGATTTGGTAGATTTTATAGTAAATGATAATGATTATTTAACACAGGCAGAAACAGAGACAGCACATGCGTTGTTTACTGCACAGGAAGCAAAACAACATAGAGATGCAGTACAGGTTC
PRIMER_LEFT_EXPLAIN=considered 8450, GC content failed 463, low tm 4051, high tm 1050, long poly-x seq 50, ok 2836
PRIMER_RIGHT_EXPLAIN=considered 8450, GC content failed 395, low tm 4046, high tm 1103, long poly-x seq 50, ok 2856
PRIMER_INTERNAL_EXPLAIN=considered 4645, GC content failed 172, low tm 106, high tm 3532, high hairpin stability 26, long poly-x seq 30, ok 779
PRIMER_PAIR_EXPLAIN=considered 75, unacceptable product size 60, no internal oligo 8, ok 7
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.202027
PRIMER_LEFT_0_PENALTY=0.036732
PRIMER_RIGHT_0_PENALTY=0.165295
PRIMER_INTERNAL_0_PENALTY=0.174524
PRIMER_LEFT_0_SEQUENCE=GTGTGCCCCATCTGTTCTCA
PRIMER_RIGHT_0_SEQUENCE=CAACGCATGTGCTGTCTCTG
PRIMER_INTERNAL_0_SEQUENCE=AGTAAATGATAATGATTATTTAACACAGGC
PRIMER_LEFT_0=688,20
PRIMER_RIGHT_0=933,20
PRIMER_INTERNAL_0=879,30
PRIMER_LEFT_0_TM=59.963
PRIMER_RIGHT_0_TM=59.835
PRIMER_INTERNAL_0_TM=60.175
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=26.667
PRIMER_INTERNAL_0_SELF_ANY_TH=2.98
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=6.08
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=39.32
PRIMER_INTERNAL_0_HAIRPIN_TH=37.34
PRIMER_LEFT_0_END_STABILITY=3.2700
PRIMER_RIGHT_0_END_STABILITY=3.3500
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=246
PRIMER_PAIR_0_PRODUCT_TM=83.0
PRIMER_PAIR_1_PENALTY=0.202027
PRIMER_LEFT_1_PENALTY=0.036732
PRIMER_RIGHT_1_PENALTY=0.165295
PRIMER_INTERNAL_1_PENALTY=0.174524
PRIMER_LEFT_1_SEQUENCE=TGTGTGCCCCATCTGTTCTC
PRIMER_RIGHT_1_SEQUENCE=CAACGCATGTGCTGTCTCTG
PRIMER_INTERNAL_1_SEQUENCE=AGTAAATGATAATGATTATTTAACACAGGC
PRIMER_LEFT_1=687,20
PRIMER_RIGHT_1=933,20
PRIMER_INTERNAL_1=879,30
PRIMER_LEFT_1_TM=59.963
PRIMER_RIGHT_1_TM=59.835
PRIMER_INTERNAL_1_TM=60.175
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=26.667
PRIMER_INTERNAL_1_SELF_ANY_TH=2.98
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=6.08
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=39.32
PRIMER_INTERNAL_1_HAIRPIN_TH=37.34
PRIMER_LEFT_1_END_STABILITY=2.8700
PRIMER_RIGHT_1_END_STABILITY=3.3500
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=247
PRIMER_PAIR_1_PRODUCT_TM=83.0
PRIMER_PAIR_2_PENALTY=0.213910
PRIMER_LEFT_2_PENALTY=0.033504
PRIMER_RIGHT_2_PENALTY=0.180406
PRIMER_INTERNAL_2_PENALTY=4.699586
PRIMER_LEFT_2_SEQUENCE=GACAAGCAGAACCGGACAGA
PRIMER_RIGHT_2_SEQUENCE=CGTACCCTCTTCCCCATTGG
PRIMER_INTERNAL_2_SEQUENCE=CTGTTCTCAGAAACCATAATCTACCATG
PRIMER_LEFT_2=548,20
PRIMER_RIGHT_2=762,20
PRIMER_INTERNAL_2=699,28
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.820
PRIMER_INTERNAL_2_TM=62.700
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=39.286
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=9.46
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=44.46
PRIMER_LEFT_2_END_STABILITY=3.4100
PRIMER_RIGHT_2_END_STABILITY=3.1600
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=215
PRIMER_PAIR_2_PRODUCT_TM=85.1
PRIMER_PAIR_3_PENALTY=0.213910
PRIMER_LEFT_3_PENALTY=0.033504
PRIMER_RIGHT_3_PENALTY=0.180406
PRIMER_INTERNAL_3_PENALTY=4.699586
PRIMER_LEFT_3_SEQUENCE=GACAAGCAGAACCGGACAGA
PRIMER_RIGHT_3_SEQUENCE=CCGTACCCTCTTCCCCATTG
PRIMER_INTERNAL_3_SEQUENCE=CTGTTCTCAGAAACCATAATCTACCATG
PRIMER_LEFT_3=548,20
PRIMER_RIGHT_3=763,20
PRIMER_INTERNAL_3=699,28
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.820
PRIMER_INTERNAL_3_TM=62.700
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=39.286
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=9.46
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=44.46
PRIMER_LEFT_3_END_STABILITY=3.4100
PRIMER_RIGHT_3_END_STABILITY=2.8200
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=216
PRIMER_PAIR_3_PRODUCT_TM=85.2
PRIMER_PAIR_4_PENALTY=0.214007
PRIMER_LEFT_4_PENALTY=0.033601
PRIMER_RIGHT_4_PENALTY=0.180406
PRIMER_INTERNAL_4_PENALTY=4.699586
PRIMER_LEFT_4_SEQUENCE=GACAGCTCAGAGGAGGAGGA
PRIMER_RIGHT_4_SEQUENCE=CGTACCCTCTTCCCCATTGG
PRIMER_INTERNAL_4_SEQUENCE=CTGTTCTCAGAAACCATAATCTACCATG
PRIMER_LEFT_4=508,20
PRIMER_RIGHT_4=762,20
PRIMER_INTERNAL_4=699,28
PRIMER_LEFT_4_TM=60.034
PRIMER_RIGHT_4_TM=59.820
PRIMER_INTERNAL_4_TM=62.700
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=39.286
PRIMER_INTERNAL_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=41.67
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=44.46
PRIMER_LEFT_4_END_STABILITY=3.7100
PRIMER_RIGHT_4_END_STABILITY=3.1600
PRIMER_PAIR_4_COMPL_ANY_TH=11.14
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=255
PRIMER_PAIR_4_PRODUCT_TM=85.9
=
PRIMER_SALT_MONOVALENT=0.0001
PRIMER_SALT_DIVALENT=2.5
PRIMER_DNA_CONC=205
PRIMER_DNTP_CONC=1.5
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_MIN_THREE_PRIME_DISTANCE=4
SEQUENCE_ID=sec_str_thal_args_def_vs_user
SEQUENCE_TEMPLATE=CGCTGCTGCGCTCCAAGCTTTACCGCTTCGCCTGCTGGCTTCGCTAAATTGCGGATTGGAAAATTTTGAAATAAGAACCCCTATCGGGCTGTTATCTGATTATGGTTATTTTGACATAGTTGTATCATCTTAAAAACAAGCATACAATCTGCAAATCTTAGACAAAGCAAAACCCCCGCCAAACGCCAATCTGCACGGGGGTTTCGAGAT
PRIMER_LEFT_EXPLAIN=considered 1109, GC content failed 19, low tm 484, high tm 303, high hairpin stability 28, long poly-x seq 2, ok 273
PRIMER_RIGHT_EXPLAIN=considered 706, GC content failed 2, low tm 460, high tm 48, high hairpin stability 2, long poly-x seq 65, ok 129
PRIMER_INTERNAL_EXPLAIN=considered 905, GC content failed 1, low tm 2, high tm 646, ok 256
PRIMER_PAIR_EXPLAIN=considered 1511, unacceptable product size 1505, primer in pair overlaps a primer in a better pair 773, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.989616
PRIMER_LEFT_0_PENALTY=0.011923
PRIMER_RIGHT_0_PENALTY=1.977693
PRIMER_INTERNAL_0_PENALTY=0.071130
PRIMER_LEFT_0_SEQUENCE=GCTCCAAGCTTTACCGCTTC
PRIMER_RIGHT_0_SEQUENCE=CGTGCAGATTGGCGTTTGG
PRIMER_INTERNAL_0_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA
PRIMER_LEFT_0=9,20
PRIMER_RIGHT_0=196,19
PRIMER_INTERNAL_0=109,30
PRIMER_LEFT_0_TM=59.988
PRIMER_RIGHT_0_TM=60.978
PRIMER_INTERNAL_0_TM=59.929
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_INTERNAL_0_GC_PERCENT=23.333
PRIMER_INTERNAL_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=45.15
PRIMER_RIGHT_0_HAIRPIN_TH=35.65
PRIMER_INTERNAL_0_HAIRPIN_TH=36.73
PRIMER_LEFT_0_END_STABILITY=3.8600
PRIMER_RIGHT_0_END_STABILITY=3.2800
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=188
PRIMER_PAIR_0_PRODUCT_TM=80.3
PRIMER_PAIR_1_PENALTY=4.000441
PRIMER_LEFT_1_PENALTY=0.267650
PRIMER_RIGHT_1_PENALTY=3.732791
PRIMER_INTERNAL_1_PENALTY=0.071130
PRIMER_LEFT_1_SEQUENCE=TGCTGGCTTCGCTAAATTGC
PRIMER_RIGHT_1_SEQUENCE=AGATTGGCGTTTGGCGGG
PRIMER_INTERNAL_1_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA
PRIMER_LEFT_1=32,20
PRIMER_RIGHT_1=191,18
PRIMER_INTERNAL_1=109,30
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=61.733
PRIMER_INTERNAL_1_TM=59.929
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_INTERNAL_1_GC_PERCENT=23.333
PRIMER_INTERNAL_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=44.25
PRIMER_INTERNAL_1_HAIRPIN_TH=36.73
PRIMER_LEFT_1_END_STABILITY=3.5600
PRIMER_RIGHT_1_END_STABILITY=6.1300
PRIMER_PAIR_1_COMPL_ANY_TH=12.57
PRIMER_PAIR_1_COMPL_END_TH=12.57
PRIMER_PAIR_1_PRODUCT_SIZE=160
PRIMER_PAIR_1_PRODUCT_TM=78.1
PRIMER_PAIR_2_PENALTY=4.722458
PRIMER_LEFT_2_PENALTY=1.255761
PRIMER_RIGHT_2_PENALTY=3.466697
PRIMER_INTERNAL_2_PENALTY=1.447512
PRIMER_LEFT_2_SEQUENCE=CTGCGCTCCAAGCTTTACC
PRIMER_RIGHT_2_SEQUENCE=ACCATAATCAGATAACAGCCCGA
PRIMER_INTERNAL_2_SEQUENCE=TAAATTGCGGATTGGAAAATTTTGAAATAA
PRIMER_LEFT_2=5,19
PRIMER_RIGHT_2=105,23
PRIMER_INTERNAL_2=44,30
PRIMER_LEFT_2_TM=59.744
PRIMER_RIGHT_2_TM=59.533
PRIMER_INTERNAL_2_TM=61.448
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_INTERNAL_2_GC_PERCENT=23.333
PRIMER_INTERNAL_2_SELF_ANY_TH=13.39
PRIMER_LEFT_2_SELF_ANY_TH=13.71
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=4.32
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=38.44
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=34.55
PRIMER_LEFT_2_END_STABILITY=2.8500
PRIMER_RIGHT_2_END_STABILITY=5.1400
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=101
PRIMER_PAIR_2_PRODUCT_TM=79.4
PRIMER_PAIR_3_PENALTY=6.437295
PRIMER_LEFT_3_PENALTY=1.557991
PRIMER_RIGHT_3_PENALTY=4.879304
PRIMER_INTERNAL_3_PENALTY=0.071130
PRIMER_LEFT_3_SEQUENCE=AAGCTTTACCGCTTCGCCT
PRIMER_RIGHT_3_SEQUENCE=GGGGTTTTGCTTTGTCTAAGAT
PRIMER_INTERNAL_3_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA
PRIMER_LEFT_3=14,19
PRIMER_RIGHT_3=175,22
PRIMER_INTERNAL_3=109,30
PRIMER_LEFT_3_TM=60.558
PRIMER_RIGHT_3_TM=57.121
PRIMER_INTERNAL_3_TM=59.929
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=23.333
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.09
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=45.15
PRIMER_RIGHT_3_HAIRPIN_TH=34.77
PRIMER_INTERNAL_3_HAIRPIN_TH=36.73
PRIMER_LEFT_3_END_STABILITY=5.5200
PRIMER_RIGHT_3_END_STABILITY=2.4000
PRIMER_PAIR_3_COMPL_ANY_TH=4.68
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=162
PRIMER_PAIR_3_PRODUCT_TM=78.2
PRIMER_PAIR_4_PENALTY=7.552603
PRIMER_LEFT_4_PENALTY=2.405360
PRIMER_RIGHT_4_PENALTY=5.147243
PRIMER_INTERNAL_4_PENALTY=0.071130
PRIMER_LEFT_4_SEQUENCE=CTTTACCGCTTCGCCTGCTG
PRIMER_RIGHT_4_SEQUENCE=GGGTTTTGCTTTGTCTAAGATTTGC
PRIMER_INTERNAL_4_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA
PRIMER_LEFT_4=17,20
PRIMER_RIGHT_4=174,25
PRIMER_INTERNAL_4=109,30
PRIMER_LEFT_4_TM=62.405
PRIMER_RIGHT_4_TM=59.853
PRIMER_INTERNAL_4_TM=59.929
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=23.333
PRIMER_INTERNAL_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.09
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=34.77
PRIMER_INTERNAL_4_HAIRPIN_TH=36.73
PRIMER_LEFT_4_END_STABILITY=4.4100
PRIMER_RIGHT_4_END_STABILITY=3.6800
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=158
PRIMER_PAIR_4_PRODUCT_TM=78.0
=
