SEQUENCE_ID=quality_test1
PRIMER_EXPLAIN_FLAG=1
P3_COMMENT=quality scores out of range
PRIMER_MIN_TM=0
PRIMER_INTERNAL_MIN_TM=0
PRIMER_INTERNAL_MAX_TM=100
PRIMER_MAX_TM=100
PRIMER_MAX_SELF_ANY=20
PRIMER_MAX_SELF_END=20
PRIMER_PAIR_MAX_COMPL_ANY=20
PRIMER_PAIR_MAX_COMPL_END=20
PRIMER_PRODUCT_SIZE_RANGE=50-70 70-90 90-110 110-500
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
PRIMER_MIN_QUALITY=30
PRIMER_QUALITY_RANGE_MAX=40
SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 
PRIMER_ERROR=Sequence quality score out of range
=
SEQUENCE_ID=quality_test2
P3_COMMENT=quality scores out of range
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 
PRIMER_MIN_QUALITY=60
PRIMER_QUALITY_RANGE_MIN=60
PRIMER_QUALITY_RANGE_MAX=65
PRIMER_ERROR=Sequence quality score out of range
=
SEQUENCE_ID=quality_test3
P3_COMMENT=quality scores out of range
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 
PRIMER_QUALITY_RANGE_MIN=0
PRIMER_MIN_QUALITY=9
PRIMER_QUALITY_RANGE_MAX=49
PRIMER_ERROR=Sequence quality score out of range
=
SEQUENCE_ID=quality_test4
P3_COMMENT=Should return exactly one pair
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
PRIMER_MIN_QUALITY=60
PRIMER_QUALITY_RANGE_MAX=70
PRIMER_MIN_SIZE=10
SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 
PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=104.232823
PRIMER_LEFT_0_PENALTY=52.299978
PRIMER_RIGHT_0_PENALTY=51.932846
PRIMER_LEFT_0_SEQUENCE=ATCGTAGCTG
PRIMER_RIGHT_0_SEQUENCE=GCATCGACTA
PRIMER_LEFT_0=0,10
PRIMER_RIGHT_0=120,10
PRIMER_LEFT_0_TM=17.700
PRIMER_RIGHT_0_TM=18.067
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=60
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=5.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=121
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
SEQUENCE_ID=quality_test5
P3_COMMENT=Should return exactly one pair
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 
PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 111, low sequence quality 110, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=104.232823
PRIMER_LEFT_0_PENALTY=52.299978
PRIMER_RIGHT_0_PENALTY=51.932846
PRIMER_INTERNAL_0_PENALTY=44.970813
PRIMER_LEFT_0_SEQUENCE=ATCGTAGCTG
PRIMER_RIGHT_0_SEQUENCE=GCATCGACTA
PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT
PRIMER_LEFT_0=0,10
PRIMER_RIGHT_0=120,10
PRIMER_INTERNAL_0=73,12
PRIMER_LEFT_0_TM=17.700
PRIMER_RIGHT_0_TM=18.067
PRIMER_INTERNAL_0_TM=23.029
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=60
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=5.4000
PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=121
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
SEQUENCE_ID=quality_test6
P3_COMMENT=Should fail because of low quality for middle oligo
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=51
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 53 54 54 54 54 50 54 54 54 54 54 51 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 
PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 110, low sequence quality 110, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=quality_test7
P3_COMMENT=Should fail as there are too few score values
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=20
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 
PRIMER_ERROR=Error in sequence quality data
=
SEQUENCE_ID=quality_test8
P3_COMMENT=Should fail as there are too many score values
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=20
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY= 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 
PRIMER_ERROR=Error in sequence quality data
=
SEQUENCE_ID=quality_test9
P3_COMMENT=Should fail because of low end quality
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
PRIMER_MIN_END_QUALITY=61
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 
PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 89, ok 0
PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 89, ok 0
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=quality_test10
P3_COMMENT=Should return exactly one pair
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
PRIMER_MIN_END_QUALITY=61
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60 
PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 111, low sequence quality 110, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=104.232823
PRIMER_LEFT_0_PENALTY=52.299978
PRIMER_RIGHT_0_PENALTY=51.932846
PRIMER_INTERNAL_0_PENALTY=44.970813
PRIMER_LEFT_0_SEQUENCE=ATCGTAGCTG
PRIMER_RIGHT_0_SEQUENCE=GCATCGACTA
PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT
PRIMER_LEFT_0=0,10
PRIMER_RIGHT_0=120,10
PRIMER_INTERNAL_0=73,12
PRIMER_LEFT_0_TM=17.700
PRIMER_RIGHT_0_TM=18.067
PRIMER_INTERNAL_0_TM=23.029
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=60
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=5.4000
PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=121
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
SEQUENCE_ID=quality_test11
P3_COMMENT=Should fail as high quality pairs are not in the included region
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
PRIMER_MIN_END_QUALITY=61
SEQUENCE_INCLUDED_REGION=2,115
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60 
PRIMER_LEFT_EXPLAIN=considered 83, low sequence quality 82, ok 1
PRIMER_RIGHT_EXPLAIN=considered 83, low sequence quality 83, ok 0
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=quality_test12
P3_COMMENT=Should return exactly one pair
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
PRIMER_MIN_END_QUALITY=61
SEQUENCE_INCLUDED_REGION=2,115
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 
PRIMER_LEFT_EXPLAIN=considered 83, low sequence quality 82, ok 1
PRIMER_RIGHT_EXPLAIN=considered 83, low sequence quality 82, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 105, low sequence quality 104, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=108.457122
PRIMER_LEFT_0_PENALTY=52.299978
PRIMER_RIGHT_0_PENALTY=56.157144
PRIMER_INTERNAL_0_PENALTY=44.970813
PRIMER_LEFT_0_SEQUENCE=CGTAGCTGAT
PRIMER_RIGHT_0_SEQUENCE=CGACTAGCTA
PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT
PRIMER_LEFT_0=2,10
PRIMER_RIGHT_0=116,10
PRIMER_INTERNAL_0=73,12
PRIMER_LEFT_0_TM=17.700
PRIMER_RIGHT_0_TM=13.843
PRIMER_INTERNAL_0_TM=23.029
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=60
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=115
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
SEQUENCE_ID=quality_test13
P3_COMMENT=Should return exactly one pair
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
PRIMER_MIN_END_QUALITY=61
SEQUENCE_INCLUDED_REGION=2,115
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=60 60 60 60.33 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 
PRIMER_ERROR=Error in sequence quality data
=
SEQUENCE_ID=quality_test14
P3_COMMENT=Should return exactly one pair
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MIN_QUALITY=55
PRIMER_INTERNAL_MIN_SIZE=12
PRIMER_MIN_END_QUALITY=61
SEQUENCE_INCLUDED_REGION=2,115
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
SEQUENCE_QUALITY=     	60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 
PRIMER_LEFT_EXPLAIN=considered 83, low sequence quality 82, ok 1
PRIMER_RIGHT_EXPLAIN=considered 83, low sequence quality 82, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 105, low sequence quality 104, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=108.457122
PRIMER_LEFT_0_PENALTY=52.299978
PRIMER_RIGHT_0_PENALTY=56.157144
PRIMER_INTERNAL_0_PENALTY=44.970813
PRIMER_LEFT_0_SEQUENCE=CGTAGCTGAT
PRIMER_RIGHT_0_SEQUENCE=CGACTAGCTA
PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT
PRIMER_LEFT_0=2,10
PRIMER_RIGHT_0=116,10
PRIMER_INTERNAL_0=73,12
PRIMER_LEFT_0_TM=17.700
PRIMER_RIGHT_0_TM=13.843
PRIMER_INTERNAL_0_TM=23.029
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=60
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=115
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
