SEQUENCE_ID=masker_default
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_EXPLAIN_FLAG=1
PRIMER_MASK_TEMPLATE=1
PRIMER_MASK_FAILURE_RATE=0.2
PRIMER_MASK_5P_DIRECTION=1
PRIMER_MASK_3P_DIRECTION=1
PRIMER_WT_MASK_FAILURE_RATE=0.0
[encoded data, 18805 characters]
PRIMER_LEFT_EXPLAIN=considered 180335, GC content failed 8877, low tm 34005, high tm 101808, high end compl 2, long poly-x seq 1117, lowercase masking of 3' end 530, ok 33996
PRIMER_RIGHT_EXPLAIN=considered 180325, GC content failed 8926, low tm 33987, high tm 101882, high end compl 1, long poly-x seq 1108, lowercase masking of 3' end 480, ok 33941
PRIMER_INTERNAL_EXPLAIN=considered 180500, GC content failed 9272, low tm 34043, high tm 102015, long poly-x seq 1142, ok 34028
PRIMER_PAIR_EXPLAIN=considered 338, unacceptable product size 331, high any compl 1, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.005611
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.005574
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=ATGCCCACCTGCTTTGTAAC
PRIMER_RIGHT_0_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_0_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_LEFT_0=2959,20
PRIMER_RIGHT_0=3388,20
PRIMER_INTERNAL_0=3094,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.994
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=430
PRIMER_PAIR_0_PRODUCT_TM=81.9
PRIMER_PAIR_1_PENALTY=0.012524
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.001978
PRIMER_INTERNAL_1_PENALTY=0.026532
PRIMER_LEFT_1_SEQUENCE=GGTTCAAGCGACTCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=ACAGGGTCTGAGTGGTTTGG
PRIMER_INTERNAL_1_SEQUENCE=AAGACTGGGTTTCACCATGC
PRIMER_LEFT_1=10633,20
PRIMER_RIGHT_1=11008,20
PRIMER_INTERNAL_1=10719,20
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.002
PRIMER_INTERNAL_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=376
PRIMER_PAIR_1_PRODUCT_TM=82.1
PRIMER_PAIR_2_PENALTY=0.016397
PRIMER_LEFT_2_PENALTY=0.001978
PRIMER_RIGHT_2_PENALTY=0.014419
PRIMER_INTERNAL_2_PENALTY=0.026532
PRIMER_LEFT_2_SEQUENCE=CCAAACCACTCAGACCCTGT
PRIMER_RIGHT_2_SEQUENCE=CAGAACTGTCTCTCCTGGGC
PRIMER_INTERNAL_2_SEQUENCE=ATCACCCTTGCCACTCAAAC
PRIMER_LEFT_2=10989,20
PRIMER_RIGHT_2=11145,20
PRIMER_INTERNAL_2=11056,20
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=59.986
PRIMER_INTERNAL_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=157
PRIMER_PAIR_2_PRODUCT_TM=78.0
PRIMER_PAIR_3_PENALTY=0.017482
PRIMER_LEFT_3_PENALTY=0.010545
PRIMER_RIGHT_3_PENALTY=0.006937
PRIMER_INTERNAL_3_PENALTY=0.066676
PRIMER_LEFT_3_SEQUENCE=GTTCTGAGGCAGAAACTCGG
PRIMER_RIGHT_3_SEQUENCE=GAGGACGAAGTCCAGCAGAC
PRIMER_INTERNAL_3_SEQUENCE=ACGGAGGAAGAGGGGAGTAA
PRIMER_LEFT_3=11140,20
PRIMER_RIGHT_3=11491,20
PRIMER_INTERNAL_3=11417,20
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=352
PRIMER_PAIR_3_PRODUCT_TM=85.8
PRIMER_PAIR_4_PENALTY=0.018045
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_INTERNAL_4_PENALTY=0.042377
PRIMER_LEFT_4_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_4_SEQUENCE=TGGGAGGTGAGGAAAATCTG
PRIMER_LEFT_4=3094,20
PRIMER_RIGHT_4=3388,20
PRIMER_INTERNAL_4=3336,20
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.994
PRIMER_INTERNAL_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=295
PRIMER_PAIR_4_PRODUCT_TM=81.2
=
SEQUENCE_ID=masker_lc_masking
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_EXPLAIN_FLAG=1
PRIMER_MASK_TEMPLATE=1
PRIMER_MASK_FAILURE_RATE=0.2
PRIMER_MASK_5P_DIRECTION=1
PRIMER_MASK_3P_DIRECTION=1
PRIMER_WT_MASK_FAILURE_RATE=0.0
PRIMER_LOWERCASE_MASKING=0
[encoded data, 18804 characters]
PRIMER_LEFT_EXPLAIN=considered 180325, GC content failed 8877, low tm 34002, high tm 101805, high end compl 2, long poly-x seq 1117, lowercase masking of 3' end 530, ok 33992
PRIMER_RIGHT_EXPLAIN=considered 180315, GC content failed 8926, low tm 33985, high tm 101878, high end compl 1, long poly-x seq 1108, lowercase masking of 3' end 480, ok 33937
PRIMER_INTERNAL_EXPLAIN=considered 180490, GC content failed 9272, low tm 34040, high tm 102012, long poly-x seq 1142, ok 34024
PRIMER_PAIR_EXPLAIN=considered 338, unacceptable product size 331, high any compl 1, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.005611
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.005574
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=ATGCCCACCTGCTTTGTAAC
PRIMER_RIGHT_0_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_0_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_LEFT_0=2958,20
PRIMER_RIGHT_0=3387,20
PRIMER_INTERNAL_0=3093,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.994
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=430
PRIMER_PAIR_0_PRODUCT_TM=81.9
PRIMER_PAIR_1_PENALTY=0.012524
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.001978
PRIMER_INTERNAL_1_PENALTY=0.026532
PRIMER_LEFT_1_SEQUENCE=GGTTCAAGCGACTCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=ACAGGGTCTGAGTGGTTTGG
PRIMER_INTERNAL_1_SEQUENCE=AAGACTGGGTTTCACCATGC
PRIMER_LEFT_1=10632,20
PRIMER_RIGHT_1=11007,20
PRIMER_INTERNAL_1=10718,20
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.002
PRIMER_INTERNAL_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=376
PRIMER_PAIR_1_PRODUCT_TM=82.1
PRIMER_PAIR_2_PENALTY=0.016397
PRIMER_LEFT_2_PENALTY=0.001978
PRIMER_RIGHT_2_PENALTY=0.014419
PRIMER_INTERNAL_2_PENALTY=0.026532
PRIMER_LEFT_2_SEQUENCE=CCAAACCACTCAGACCCTGT
PRIMER_RIGHT_2_SEQUENCE=CAGAACTGTCTCTCCTGGGC
PRIMER_INTERNAL_2_SEQUENCE=ATCACCCTTGCCACTCAAAC
PRIMER_LEFT_2=10988,20
PRIMER_RIGHT_2=11144,20
PRIMER_INTERNAL_2=11055,20
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=59.986
PRIMER_INTERNAL_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=157
PRIMER_PAIR_2_PRODUCT_TM=78.0
PRIMER_PAIR_3_PENALTY=0.017482
PRIMER_LEFT_3_PENALTY=0.010545
PRIMER_RIGHT_3_PENALTY=0.006937
PRIMER_INTERNAL_3_PENALTY=0.066676
PRIMER_LEFT_3_SEQUENCE=GTTCTGAGGCAGAAACTCGG
PRIMER_RIGHT_3_SEQUENCE=GAGGACGAAGTCCAGCAGAC
PRIMER_INTERNAL_3_SEQUENCE=ACGGAGGAAGAGGGGAGTAA
PRIMER_LEFT_3=11139,20
PRIMER_RIGHT_3=11490,20
PRIMER_INTERNAL_3=11416,20
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=352
PRIMER_PAIR_3_PRODUCT_TM=85.8
PRIMER_PAIR_4_PENALTY=0.018045
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_INTERNAL_4_PENALTY=0.042377
PRIMER_LEFT_4_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_4_SEQUENCE=TGGGAGGTGAGGAAAATCTG
PRIMER_LEFT_4=3093,20
PRIMER_RIGHT_4=3387,20
PRIMER_INTERNAL_4=3335,20
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.994
PRIMER_INTERNAL_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=295
PRIMER_PAIR_4_PRODUCT_TM=81.2
=
SEQUENCE_ID=masker_prefix1
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_EXPLAIN_FLAG=1
PRIMER_MASK_TEMPLATE=1
PRIMER_MASK_FAILURE_RATE=0.2
PRIMER_MASK_5P_DIRECTION=1
PRIMER_MASK_3P_DIRECTION=1
PRIMER_WT_MASK_FAILURE_RATE=0.0
PRIMER_MASK_KMERLIST_PREFIX=homo_sapiens
[encoded data, 18805 characters]
PRIMER_LEFT_EXPLAIN=considered 180335, GC content failed 8877, low tm 34005, high tm 101808, high end compl 2, long poly-x seq 1117, lowercase masking of 3' end 530, ok 33996
PRIMER_RIGHT_EXPLAIN=considered 180325, GC content failed 8926, low tm 33987, high tm 101882, high end compl 1, long poly-x seq 1108, lowercase masking of 3' end 480, ok 33941
PRIMER_INTERNAL_EXPLAIN=considered 180500, GC content failed 9272, low tm 34043, high tm 102015, long poly-x seq 1142, ok 34028
PRIMER_PAIR_EXPLAIN=considered 338, unacceptable product size 331, high any compl 1, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.005611
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.005574
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=ATGCCCACCTGCTTTGTAAC
PRIMER_RIGHT_0_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_0_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_LEFT_0=2959,20
PRIMER_RIGHT_0=3388,20
PRIMER_INTERNAL_0=3094,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.994
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=430
PRIMER_PAIR_0_PRODUCT_TM=81.9
PRIMER_PAIR_1_PENALTY=0.012524
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.001978
PRIMER_INTERNAL_1_PENALTY=0.026532
PRIMER_LEFT_1_SEQUENCE=GGTTCAAGCGACTCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=ACAGGGTCTGAGTGGTTTGG
PRIMER_INTERNAL_1_SEQUENCE=AAGACTGGGTTTCACCATGC
PRIMER_LEFT_1=10633,20
PRIMER_RIGHT_1=11008,20
PRIMER_INTERNAL_1=10719,20
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.002
PRIMER_INTERNAL_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=376
PRIMER_PAIR_1_PRODUCT_TM=82.1
PRIMER_PAIR_2_PENALTY=0.016397
PRIMER_LEFT_2_PENALTY=0.001978
PRIMER_RIGHT_2_PENALTY=0.014419
PRIMER_INTERNAL_2_PENALTY=0.026532
PRIMER_LEFT_2_SEQUENCE=CCAAACCACTCAGACCCTGT
PRIMER_RIGHT_2_SEQUENCE=CAGAACTGTCTCTCCTGGGC
PRIMER_INTERNAL_2_SEQUENCE=ATCACCCTTGCCACTCAAAC
PRIMER_LEFT_2=10989,20
PRIMER_RIGHT_2=11145,20
PRIMER_INTERNAL_2=11056,20
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=59.986
PRIMER_INTERNAL_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=157
PRIMER_PAIR_2_PRODUCT_TM=78.0
PRIMER_PAIR_3_PENALTY=0.017482
PRIMER_LEFT_3_PENALTY=0.010545
PRIMER_RIGHT_3_PENALTY=0.006937
PRIMER_INTERNAL_3_PENALTY=0.066676
PRIMER_LEFT_3_SEQUENCE=GTTCTGAGGCAGAAACTCGG
PRIMER_RIGHT_3_SEQUENCE=GAGGACGAAGTCCAGCAGAC
PRIMER_INTERNAL_3_SEQUENCE=ACGGAGGAAGAGGGGAGTAA
PRIMER_LEFT_3=11140,20
PRIMER_RIGHT_3=11491,20
PRIMER_INTERNAL_3=11417,20
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=352
PRIMER_PAIR_3_PRODUCT_TM=85.8
PRIMER_PAIR_4_PENALTY=0.018045
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_INTERNAL_4_PENALTY=0.042377
PRIMER_LEFT_4_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_4_SEQUENCE=TGGGAGGTGAGGAAAATCTG
PRIMER_LEFT_4=3094,20
PRIMER_RIGHT_4=3388,20
PRIMER_INTERNAL_4=3336,20
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.994
PRIMER_INTERNAL_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=295
PRIMER_PAIR_4_PRODUCT_TM=81.2
=
SEQUENCE_ID=masker_prefix2
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_EXPLAIN_FLAG=1
PRIMER_MASK_TEMPLATE=1
PRIMER_MASK_FAILURE_RATE=0.2
PRIMER_MASK_5P_DIRECTION=1
PRIMER_MASK_3P_DIRECTION=1
PRIMER_WT_MASK_FAILURE_RATE=0.0
PRIMER_MASK_KMERLIST_PREFIX=mus_musculus
[encoded data, 18805 characters]
PRIMER_LEFT_EXPLAIN=considered 180225, GC content failed 9272, low tm 34005, high tm 101808, high end compl 2, long poly-x seq 1142, ok 33996
PRIMER_RIGHT_EXPLAIN=considered 180230, GC content failed 9290, low tm 33987, high tm 101882, high end compl 1, long poly-x seq 1129, ok 33941
PRIMER_INTERNAL_EXPLAIN=considered 180500, GC content failed 9272, low tm 34043, high tm 102015, long poly-x seq 1142, ok 34028
PRIMER_PAIR_EXPLAIN=considered 338, unacceptable product size 331, high any compl 1, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.005611
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.005574
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=ATGCCCACCTGCTTTGTAAC
PRIMER_RIGHT_0_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_0_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_LEFT_0=2959,20
PRIMER_RIGHT_0=3388,20
PRIMER_INTERNAL_0=3094,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.994
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=430
PRIMER_PAIR_0_PRODUCT_TM=81.9
PRIMER_PAIR_1_PENALTY=0.012524
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.001978
PRIMER_INTERNAL_1_PENALTY=0.026532
PRIMER_LEFT_1_SEQUENCE=GGTTCAAGCGACTCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=ACAGGGTCTGAGTGGTTTGG
PRIMER_INTERNAL_1_SEQUENCE=AAGACTGGGTTTCACCATGC
PRIMER_LEFT_1=10633,20
PRIMER_RIGHT_1=11008,20
PRIMER_INTERNAL_1=10719,20
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.002
PRIMER_INTERNAL_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=376
PRIMER_PAIR_1_PRODUCT_TM=82.1
PRIMER_PAIR_2_PENALTY=0.016397
PRIMER_LEFT_2_PENALTY=0.001978
PRIMER_RIGHT_2_PENALTY=0.014419
PRIMER_INTERNAL_2_PENALTY=0.026532
PRIMER_LEFT_2_SEQUENCE=CCAAACCACTCAGACCCTGT
PRIMER_RIGHT_2_SEQUENCE=CAGAACTGTCTCTCCTGGGC
PRIMER_INTERNAL_2_SEQUENCE=ATCACCCTTGCCACTCAAAC
PRIMER_LEFT_2=10989,20
PRIMER_RIGHT_2=11145,20
PRIMER_INTERNAL_2=11056,20
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=59.986
PRIMER_INTERNAL_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=157
PRIMER_PAIR_2_PRODUCT_TM=78.0
PRIMER_PAIR_3_PENALTY=0.017482
PRIMER_LEFT_3_PENALTY=0.010545
PRIMER_RIGHT_3_PENALTY=0.006937
PRIMER_INTERNAL_3_PENALTY=0.066676
PRIMER_LEFT_3_SEQUENCE=GTTCTGAGGCAGAAACTCGG
PRIMER_RIGHT_3_SEQUENCE=GAGGACGAAGTCCAGCAGAC
PRIMER_INTERNAL_3_SEQUENCE=ACGGAGGAAGAGGGGAGTAA
PRIMER_LEFT_3=11140,20
PRIMER_RIGHT_3=11491,20
PRIMER_INTERNAL_3=11417,20
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=352
PRIMER_PAIR_3_PRODUCT_TM=85.8
PRIMER_PAIR_4_PENALTY=0.018045
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_INTERNAL_4_PENALTY=0.042377
PRIMER_LEFT_4_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_4_SEQUENCE=TGGGAGGTGAGGAAAATCTG
PRIMER_LEFT_4=3094,20
PRIMER_RIGHT_4=3388,20
PRIMER_INTERNAL_4=3336,20
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.994
PRIMER_INTERNAL_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=295
PRIMER_PAIR_4_PRODUCT_TM=81.2
=
SEQUENCE_ID=masker_path1
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_EXPLAIN_FLAG=1
PRIMER_MASK_TEMPLATE=1
PRIMER_MASK_FAILURE_RATE=0.2
PRIMER_MASK_5P_DIRECTION=1
PRIMER_MASK_3P_DIRECTION=1
PRIMER_WT_MASK_FAILURE_RATE=0.0
PRIMER_LOWERCASE_MASKING=0
PRIMER_MASK_KMERLIST_PATH=../kmer_lists/
[encoded data, 18805 characters]
PRIMER_LEFT_EXPLAIN=considered 180225, GC content failed 9272, low tm 34005, high tm 101808, high end compl 2, long poly-x seq 1142, ok 33996
PRIMER_RIGHT_EXPLAIN=considered 180230, GC content failed 9290, low tm 33987, high tm 101882, high end compl 1, long poly-x seq 1129, ok 33941
PRIMER_INTERNAL_EXPLAIN=considered 180500, GC content failed 9272, low tm 34043, high tm 102015, long poly-x seq 1142, ok 34028
PRIMER_PAIR_EXPLAIN=considered 338, unacceptable product size 331, high any compl 1, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.005611
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.005574
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=ATGCCCACCTGCTTTGTAAC
PRIMER_RIGHT_0_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_0_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_LEFT_0=2959,20
PRIMER_RIGHT_0=3388,20
PRIMER_INTERNAL_0=3094,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.994
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=430
PRIMER_PAIR_0_PRODUCT_TM=81.9
PRIMER_PAIR_1_PENALTY=0.012524
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.001978
PRIMER_INTERNAL_1_PENALTY=0.026532
PRIMER_LEFT_1_SEQUENCE=GGTTCAAGCGACTCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=ACAGGGTCTGAGTGGTTTGG
PRIMER_INTERNAL_1_SEQUENCE=AAGACTGGGTTTCACCATGC
PRIMER_LEFT_1=10633,20
PRIMER_RIGHT_1=11008,20
PRIMER_INTERNAL_1=10719,20
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.002
PRIMER_INTERNAL_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=376
PRIMER_PAIR_1_PRODUCT_TM=82.1
PRIMER_PAIR_2_PENALTY=0.016397
PRIMER_LEFT_2_PENALTY=0.001978
PRIMER_RIGHT_2_PENALTY=0.014419
PRIMER_INTERNAL_2_PENALTY=0.026532
PRIMER_LEFT_2_SEQUENCE=CCAAACCACTCAGACCCTGT
PRIMER_RIGHT_2_SEQUENCE=CAGAACTGTCTCTCCTGGGC
PRIMER_INTERNAL_2_SEQUENCE=ATCACCCTTGCCACTCAAAC
PRIMER_LEFT_2=10989,20
PRIMER_RIGHT_2=11145,20
PRIMER_INTERNAL_2=11056,20
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=59.986
PRIMER_INTERNAL_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=157
PRIMER_PAIR_2_PRODUCT_TM=78.0
PRIMER_PAIR_3_PENALTY=0.017482
PRIMER_LEFT_3_PENALTY=0.010545
PRIMER_RIGHT_3_PENALTY=0.006937
PRIMER_INTERNAL_3_PENALTY=0.066676
PRIMER_LEFT_3_SEQUENCE=GTTCTGAGGCAGAAACTCGG
PRIMER_RIGHT_3_SEQUENCE=GAGGACGAAGTCCAGCAGAC
PRIMER_INTERNAL_3_SEQUENCE=ACGGAGGAAGAGGGGAGTAA
PRIMER_LEFT_3=11140,20
PRIMER_RIGHT_3=11491,20
PRIMER_INTERNAL_3=11417,20
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=352
PRIMER_PAIR_3_PRODUCT_TM=85.8
PRIMER_PAIR_4_PENALTY=0.018045
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_INTERNAL_4_PENALTY=0.042377
PRIMER_LEFT_4_SEQUENCE=GCACACAAACCACCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=AGACCAGTTAGCTGGGGGTT
PRIMER_INTERNAL_4_SEQUENCE=TGGGAGGTGAGGAAAATCTG
PRIMER_LEFT_4=3094,20
PRIMER_RIGHT_4=3388,20
PRIMER_INTERNAL_4=3336,20
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.994
PRIMER_INTERNAL_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=295
PRIMER_PAIR_4_PRODUCT_TM=81.2
=
