SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs
PRIMER_PRODUCT_SIZE_RANGE=100-500
PRIMER_MIN_TM=0
PRIMER_MAX_TM=100
PRIMER_NUM_RETURN=40
PRIMER_MAX_SELF_ANY=100
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
PRIMER_LEFT_NUM_RETURNED=11
PRIMER_RIGHT_NUM_RETURNED=11
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=11
PRIMER_PAIR_0_PENALTY=15.194088
PRIMER_LEFT_0_PENALTY=13.434466
PRIMER_RIGHT_0_PENALTY=1.759622
PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=46.566
PRIMER_RIGHT_0_TM=58.240
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=61.5
PRIMER_PAIR_1_PENALTY=15.890689
PRIMER_LEFT_1_PENALTY=13.434466
PRIMER_RIGHT_1_PENALTY=2.456224
PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=137,20
PRIMER_LEFT_1_TM=46.566
PRIMER_RIGHT_1_TM=57.544
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=60.9
PRIMER_PAIR_2_PENALTY=16.028766
PRIMER_LEFT_2_PENALTY=13.434466
PRIMER_RIGHT_2_PENALTY=2.594300
PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=132,20
PRIMER_LEFT_2_TM=46.566
PRIMER_RIGHT_2_TM=57.406
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=133
PRIMER_PAIR_2_PRODUCT_TM=60.3
PRIMER_PAIR_3_PENALTY=16.041439
PRIMER_LEFT_3_PENALTY=13.434466
PRIMER_RIGHT_3_PENALTY=2.606973
PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTG
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=138,20
PRIMER_LEFT_3_TM=46.566
PRIMER_RIGHT_3_TM=57.393
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=139
PRIMER_PAIR_3_PRODUCT_TM=61.2
PRIMER_PAIR_4_PENALTY=16.088078
PRIMER_LEFT_4_PENALTY=13.434466
PRIMER_RIGHT_4_PENALTY=2.653612
PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_4_SEQUENCE=TTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=134,20
PRIMER_LEFT_4_TM=46.566
PRIMER_RIGHT_4_TM=57.346
PRIMER_LEFT_4_GC_PERCENT=25.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=135
PRIMER_PAIR_4_PRODUCT_TM=60.3
PRIMER_PAIR_5_PENALTY=16.511012
PRIMER_LEFT_5_PENALTY=13.434466
PRIMER_RIGHT_5_PENALTY=3.076546
PRIMER_LEFT_5_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_5_SEQUENCE=TTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_5=0,20
PRIMER_RIGHT_5=133,20
PRIMER_LEFT_5_TM=46.566
PRIMER_RIGHT_5_TM=56.923
PRIMER_LEFT_5_GC_PERCENT=25.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_END_STABILITY=5.8000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=134
PRIMER_PAIR_5_PRODUCT_TM=60.3
PRIMER_PAIR_6_PENALTY=18.133199
PRIMER_LEFT_6_PENALTY=13.434466
PRIMER_RIGHT_6_PENALTY=4.698733
PRIMER_LEFT_6_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_6=0,20
PRIMER_RIGHT_6=131,20
PRIMER_LEFT_6_TM=46.566
PRIMER_RIGHT_6_TM=55.301
PRIMER_LEFT_6_GC_PERCENT=25.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=5.8000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=132
PRIMER_PAIR_6_PRODUCT_TM=60.3
PRIMER_PAIR_7_PENALTY=18.619053
PRIMER_LEFT_7_PENALTY=13.434466
PRIMER_RIGHT_7_PENALTY=5.184587
PRIMER_LEFT_7_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_7_SEQUENCE=ATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_7=0,20
PRIMER_RIGHT_7=135,20
PRIMER_LEFT_7_TM=46.566
PRIMER_RIGHT_7_TM=54.815
PRIMER_LEFT_7_GC_PERCENT=25.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_END_STABILITY=5.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=136
PRIMER_PAIR_7_PRODUCT_TM=60.3
PRIMER_PAIR_8_PENALTY=18.619053
PRIMER_LEFT_8_PENALTY=13.434466
PRIMER_RIGHT_8_PENALTY=5.184587
PRIMER_LEFT_8_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_8_SEQUENCE=GATTTGCAAGAGACTTTGCT
PRIMER_LEFT_8=0,20
PRIMER_RIGHT_8=136,20
PRIMER_LEFT_8_TM=46.566
PRIMER_RIGHT_8_TM=54.815
PRIMER_LEFT_8_GC_PERCENT=25.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=5.00
PRIMER_LEFT_8_END_STABILITY=5.8000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=4.00
PRIMER_PAIR_8_PRODUCT_SIZE=137
PRIMER_PAIR_8_PRODUCT_TM=60.6
PRIMER_PAIR_9_PENALTY=19.337053
PRIMER_LEFT_9_PENALTY=13.434466
PRIMER_RIGHT_9_PENALTY=5.902587
PRIMER_LEFT_9_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_9_SEQUENCE=CAAGAGACTTTGCTCAGACA
PRIMER_LEFT_9=0,20
PRIMER_RIGHT_9=130,20
PRIMER_LEFT_9_TM=46.566
PRIMER_RIGHT_9_TM=54.097
PRIMER_LEFT_9_GC_PERCENT=25.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=5.8000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=4.00
PRIMER_PAIR_9_PRODUCT_SIZE=131
PRIMER_PAIR_9_PRODUCT_TM=60.0
PRIMER_PAIR_10_PENALTY=21.454739
PRIMER_LEFT_10_PENALTY=13.434466
PRIMER_RIGHT_10_PENALTY=8.020273
PRIMER_LEFT_10_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_10_SEQUENCE=AAGAGACTTTGCTCAGACAC
PRIMER_LEFT_10=0,20
PRIMER_RIGHT_10=129,20
PRIMER_LEFT_10_TM=46.566
PRIMER_RIGHT_10_TM=51.980
PRIMER_LEFT_10_GC_PERCENT=25.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_END_STABILITY=5.8000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=4.00
PRIMER_PAIR_10_PRODUCT_SIZE=130
PRIMER_PAIR_10_PRODUCT_TM=59.7
=
SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0 - same results as for -1
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
PRIMER_LEFT_NUM_RETURNED=11
PRIMER_RIGHT_NUM_RETURNED=11
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=11
PRIMER_PAIR_0_PENALTY=15.194088
PRIMER_LEFT_0_PENALTY=13.434466
PRIMER_RIGHT_0_PENALTY=1.759622
PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=46.566
PRIMER_RIGHT_0_TM=58.240
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=61.5
PRIMER_PAIR_1_PENALTY=15.890689
PRIMER_LEFT_1_PENALTY=13.434466
PRIMER_RIGHT_1_PENALTY=2.456224
PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=137,20
PRIMER_LEFT_1_TM=46.566
PRIMER_RIGHT_1_TM=57.544
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=60.9
PRIMER_PAIR_2_PENALTY=16.028766
PRIMER_LEFT_2_PENALTY=13.434466
PRIMER_RIGHT_2_PENALTY=2.594300
PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=132,20
PRIMER_LEFT_2_TM=46.566
PRIMER_RIGHT_2_TM=57.406
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=133
PRIMER_PAIR_2_PRODUCT_TM=60.3
PRIMER_PAIR_3_PENALTY=16.041439
PRIMER_LEFT_3_PENALTY=13.434466
PRIMER_RIGHT_3_PENALTY=2.606973
PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTG
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=138,20
PRIMER_LEFT_3_TM=46.566
PRIMER_RIGHT_3_TM=57.393
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=139
PRIMER_PAIR_3_PRODUCT_TM=61.2
PRIMER_PAIR_4_PENALTY=16.088078
PRIMER_LEFT_4_PENALTY=13.434466
PRIMER_RIGHT_4_PENALTY=2.653612
PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_4_SEQUENCE=TTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=134,20
PRIMER_LEFT_4_TM=46.566
PRIMER_RIGHT_4_TM=57.346
PRIMER_LEFT_4_GC_PERCENT=25.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=135
PRIMER_PAIR_4_PRODUCT_TM=60.3
PRIMER_PAIR_5_PENALTY=16.511012
PRIMER_LEFT_5_PENALTY=13.434466
PRIMER_RIGHT_5_PENALTY=3.076546
PRIMER_LEFT_5_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_5_SEQUENCE=TTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_5=0,20
PRIMER_RIGHT_5=133,20
PRIMER_LEFT_5_TM=46.566
PRIMER_RIGHT_5_TM=56.923
PRIMER_LEFT_5_GC_PERCENT=25.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_END_STABILITY=5.8000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=134
PRIMER_PAIR_5_PRODUCT_TM=60.3
PRIMER_PAIR_6_PENALTY=18.133199
PRIMER_LEFT_6_PENALTY=13.434466
PRIMER_RIGHT_6_PENALTY=4.698733
PRIMER_LEFT_6_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_6=0,20
PRIMER_RIGHT_6=131,20
PRIMER_LEFT_6_TM=46.566
PRIMER_RIGHT_6_TM=55.301
PRIMER_LEFT_6_GC_PERCENT=25.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=5.8000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=132
PRIMER_PAIR_6_PRODUCT_TM=60.3
PRIMER_PAIR_7_PENALTY=18.619053
PRIMER_LEFT_7_PENALTY=13.434466
PRIMER_RIGHT_7_PENALTY=5.184587
PRIMER_LEFT_7_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_7_SEQUENCE=ATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_7=0,20
PRIMER_RIGHT_7=135,20
PRIMER_LEFT_7_TM=46.566
PRIMER_RIGHT_7_TM=54.815
PRIMER_LEFT_7_GC_PERCENT=25.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_END_STABILITY=5.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=136
PRIMER_PAIR_7_PRODUCT_TM=60.3
PRIMER_PAIR_8_PENALTY=18.619053
PRIMER_LEFT_8_PENALTY=13.434466
PRIMER_RIGHT_8_PENALTY=5.184587
PRIMER_LEFT_8_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_8_SEQUENCE=GATTTGCAAGAGACTTTGCT
PRIMER_LEFT_8=0,20
PRIMER_RIGHT_8=136,20
PRIMER_LEFT_8_TM=46.566
PRIMER_RIGHT_8_TM=54.815
PRIMER_LEFT_8_GC_PERCENT=25.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=5.00
PRIMER_LEFT_8_END_STABILITY=5.8000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=4.00
PRIMER_PAIR_8_PRODUCT_SIZE=137
PRIMER_PAIR_8_PRODUCT_TM=60.6
PRIMER_PAIR_9_PENALTY=19.337053
PRIMER_LEFT_9_PENALTY=13.434466
PRIMER_RIGHT_9_PENALTY=5.902587
PRIMER_LEFT_9_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_9_SEQUENCE=CAAGAGACTTTGCTCAGACA
PRIMER_LEFT_9=0,20
PRIMER_RIGHT_9=130,20
PRIMER_LEFT_9_TM=46.566
PRIMER_RIGHT_9_TM=54.097
PRIMER_LEFT_9_GC_PERCENT=25.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=5.8000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=4.00
PRIMER_PAIR_9_PRODUCT_SIZE=131
PRIMER_PAIR_9_PRODUCT_TM=60.0
PRIMER_PAIR_10_PENALTY=21.454739
PRIMER_LEFT_10_PENALTY=13.434466
PRIMER_RIGHT_10_PENALTY=8.020273
PRIMER_LEFT_10_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_10_SEQUENCE=AAGAGACTTTGCTCAGACAC
PRIMER_LEFT_10=0,20
PRIMER_RIGHT_10=129,20
PRIMER_LEFT_10_TM=46.566
PRIMER_RIGHT_10_TM=51.980
PRIMER_LEFT_10_GC_PERCENT=25.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_END_STABILITY=5.8000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=4.00
PRIMER_PAIR_10_PRODUCT_SIZE=130
PRIMER_PAIR_10_PRODUCT_TM=59.7
=
SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
PRIMER_LEFT_NUM_RETURNED=11
PRIMER_RIGHT_NUM_RETURNED=11
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=11
PRIMER_PAIR_0_PENALTY=15.194088
PRIMER_LEFT_0_PENALTY=13.434466
PRIMER_RIGHT_0_PENALTY=1.759622
PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=46.566
PRIMER_RIGHT_0_TM=58.240
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=61.5
PRIMER_PAIR_1_PENALTY=15.890689
PRIMER_LEFT_1_PENALTY=13.434466
PRIMER_RIGHT_1_PENALTY=2.456224
PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=137,20
PRIMER_LEFT_1_TM=46.566
PRIMER_RIGHT_1_TM=57.544
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=60.9
PRIMER_PAIR_2_PENALTY=16.028766
PRIMER_LEFT_2_PENALTY=13.434466
PRIMER_RIGHT_2_PENALTY=2.594300
PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=132,20
PRIMER_LEFT_2_TM=46.566
PRIMER_RIGHT_2_TM=57.406
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=133
PRIMER_PAIR_2_PRODUCT_TM=60.3
PRIMER_PAIR_3_PENALTY=16.041439
PRIMER_LEFT_3_PENALTY=13.434466
PRIMER_RIGHT_3_PENALTY=2.606973
PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTG
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=138,20
PRIMER_LEFT_3_TM=46.566
PRIMER_RIGHT_3_TM=57.393
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=139
PRIMER_PAIR_3_PRODUCT_TM=61.2
PRIMER_PAIR_4_PENALTY=16.088078
PRIMER_LEFT_4_PENALTY=13.434466
PRIMER_RIGHT_4_PENALTY=2.653612
PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_4_SEQUENCE=TTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=134,20
PRIMER_LEFT_4_TM=46.566
PRIMER_RIGHT_4_TM=57.346
PRIMER_LEFT_4_GC_PERCENT=25.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=135
PRIMER_PAIR_4_PRODUCT_TM=60.3
PRIMER_PAIR_5_PENALTY=16.511012
PRIMER_LEFT_5_PENALTY=13.434466
PRIMER_RIGHT_5_PENALTY=3.076546
PRIMER_LEFT_5_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_5_SEQUENCE=TTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_5=0,20
PRIMER_RIGHT_5=133,20
PRIMER_LEFT_5_TM=46.566
PRIMER_RIGHT_5_TM=56.923
PRIMER_LEFT_5_GC_PERCENT=25.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_END_STABILITY=5.8000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=134
PRIMER_PAIR_5_PRODUCT_TM=60.3
PRIMER_PAIR_6_PENALTY=18.133199
PRIMER_LEFT_6_PENALTY=13.434466
PRIMER_RIGHT_6_PENALTY=4.698733
PRIMER_LEFT_6_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_6=0,20
PRIMER_RIGHT_6=131,20
PRIMER_LEFT_6_TM=46.566
PRIMER_RIGHT_6_TM=55.301
PRIMER_LEFT_6_GC_PERCENT=25.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=5.8000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=132
PRIMER_PAIR_6_PRODUCT_TM=60.3
PRIMER_PAIR_7_PENALTY=18.619053
PRIMER_LEFT_7_PENALTY=13.434466
PRIMER_RIGHT_7_PENALTY=5.184587
PRIMER_LEFT_7_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_7_SEQUENCE=ATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_7=0,20
PRIMER_RIGHT_7=135,20
PRIMER_LEFT_7_TM=46.566
PRIMER_RIGHT_7_TM=54.815
PRIMER_LEFT_7_GC_PERCENT=25.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_END_STABILITY=5.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=136
PRIMER_PAIR_7_PRODUCT_TM=60.3
PRIMER_PAIR_8_PENALTY=18.619053
PRIMER_LEFT_8_PENALTY=13.434466
PRIMER_RIGHT_8_PENALTY=5.184587
PRIMER_LEFT_8_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_8_SEQUENCE=GATTTGCAAGAGACTTTGCT
PRIMER_LEFT_8=0,20
PRIMER_RIGHT_8=136,20
PRIMER_LEFT_8_TM=46.566
PRIMER_RIGHT_8_TM=54.815
PRIMER_LEFT_8_GC_PERCENT=25.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=5.00
PRIMER_LEFT_8_END_STABILITY=5.8000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=4.00
PRIMER_PAIR_8_PRODUCT_SIZE=137
PRIMER_PAIR_8_PRODUCT_TM=60.6
PRIMER_PAIR_9_PENALTY=19.337053
PRIMER_LEFT_9_PENALTY=13.434466
PRIMER_RIGHT_9_PENALTY=5.902587
PRIMER_LEFT_9_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_9_SEQUENCE=CAAGAGACTTTGCTCAGACA
PRIMER_LEFT_9=0,20
PRIMER_RIGHT_9=130,20
PRIMER_LEFT_9_TM=46.566
PRIMER_RIGHT_9_TM=54.097
PRIMER_LEFT_9_GC_PERCENT=25.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=5.8000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=4.00
PRIMER_PAIR_9_PRODUCT_SIZE=131
PRIMER_PAIR_9_PRODUCT_TM=60.0
PRIMER_PAIR_10_PENALTY=21.454739
PRIMER_LEFT_10_PENALTY=13.434466
PRIMER_RIGHT_10_PENALTY=8.020273
PRIMER_LEFT_10_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_10_SEQUENCE=AAGAGACTTTGCTCAGACAC
PRIMER_LEFT_10=0,20
PRIMER_RIGHT_10=129,20
PRIMER_LEFT_10_TM=46.566
PRIMER_RIGHT_10_TM=51.980
PRIMER_LEFT_10_GC_PERCENT=25.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_END_STABILITY=5.8000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=4.00
PRIMER_PAIR_10_PRODUCT_SIZE=130
PRIMER_PAIR_10_PRODUCT_TM=59.7
=
SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2 - 5 pairs
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=15.194088
PRIMER_LEFT_0_PENALTY=13.434466
PRIMER_RIGHT_0_PENALTY=1.759622
PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=46.566
PRIMER_RIGHT_0_TM=58.240
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=61.5
PRIMER_PAIR_1_PENALTY=15.890689
PRIMER_LEFT_1_PENALTY=13.434466
PRIMER_RIGHT_1_PENALTY=2.456224
PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=137,20
PRIMER_LEFT_1_TM=46.566
PRIMER_RIGHT_1_TM=57.544
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=60.9
PRIMER_PAIR_2_PENALTY=16.028766
PRIMER_LEFT_2_PENALTY=13.434466
PRIMER_RIGHT_2_PENALTY=2.594300
PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=132,20
PRIMER_LEFT_2_TM=46.566
PRIMER_RIGHT_2_TM=57.406
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=133
PRIMER_PAIR_2_PRODUCT_TM=60.3
PRIMER_PAIR_3_PENALTY=16.088078
PRIMER_LEFT_3_PENALTY=13.434466
PRIMER_RIGHT_3_PENALTY=2.653612
PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_3_SEQUENCE=TTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=134,20
PRIMER_LEFT_3_TM=46.566
PRIMER_RIGHT_3_TM=57.346
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=135
PRIMER_PAIR_3_PRODUCT_TM=60.3
PRIMER_PAIR_4_PENALTY=19.337053
PRIMER_LEFT_4_PENALTY=13.434466
PRIMER_RIGHT_4_PENALTY=5.902587
PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_4_SEQUENCE=CAAGAGACTTTGCTCAGACA
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=130,20
PRIMER_LEFT_4_TM=46.566
PRIMER_RIGHT_4_TM=54.097
PRIMER_LEFT_4_GC_PERCENT=25.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=131
PRIMER_PAIR_4_PRODUCT_TM=60.0
=
SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3 - 4 pairs
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
PRIMER_LEFT_NUM_RETURNED=4
PRIMER_RIGHT_NUM_RETURNED=4
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=4
PRIMER_PAIR_0_PENALTY=15.194088
PRIMER_LEFT_0_PENALTY=13.434466
PRIMER_RIGHT_0_PENALTY=1.759622
PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=46.566
PRIMER_RIGHT_0_TM=58.240
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=61.5
PRIMER_PAIR_1_PENALTY=16.028766
PRIMER_LEFT_1_PENALTY=13.434466
PRIMER_RIGHT_1_PENALTY=2.594300
PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_1_SEQUENCE=TGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=132,20
PRIMER_LEFT_1_TM=46.566
PRIMER_RIGHT_1_TM=57.406
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=133
PRIMER_PAIR_1_PRODUCT_TM=60.3
PRIMER_PAIR_2_PENALTY=18.619053
PRIMER_LEFT_2_PENALTY=13.434466
PRIMER_RIGHT_2_PENALTY=5.184587
PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_2_SEQUENCE=ATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=135,20
PRIMER_LEFT_2_TM=46.566
PRIMER_RIGHT_2_TM=54.815
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=136
PRIMER_PAIR_2_PRODUCT_TM=60.3
PRIMER_PAIR_3_PENALTY=21.454739
PRIMER_LEFT_3_PENALTY=13.434466
PRIMER_RIGHT_3_PENALTY=8.020273
PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT
PRIMER_RIGHT_3_SEQUENCE=AAGAGACTTTGCTCAGACAC
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=129,20
PRIMER_LEFT_3_TM=46.566
PRIMER_RIGHT_3_TM=51.980
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=130
PRIMER_PAIR_3_PRODUCT_TM=59.7
=
SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=16.938641
PRIMER_LEFT_0_PENALTY=9.244200
PRIMER_RIGHT_0_PENALTY=7.694441
PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT
PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=50.756
PRIMER_RIGHT_0_TM=52.306
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=35.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=59.4
=
SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0 - same results as for -1
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=16.938641
PRIMER_LEFT_0_PENALTY=9.244200
PRIMER_RIGHT_0_PENALTY=7.694441
PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT
PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=50.756
PRIMER_RIGHT_0_TM=52.306
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=35.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=59.4
=
SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=16.938641
PRIMER_LEFT_0_PENALTY=9.244200
PRIMER_RIGHT_0_PENALTY=7.694441
PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT
PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=50.756
PRIMER_RIGHT_0_TM=52.306
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=35.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=59.4
=
SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2 - 5 pairs
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=16.938641
PRIMER_LEFT_0_PENALTY=9.244200
PRIMER_RIGHT_0_PENALTY=7.694441
PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT
PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=50.756
PRIMER_RIGHT_0_TM=52.306
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=35.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=59.4
=
SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3 - 3 pairs
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=16.938641
PRIMER_LEFT_0_PENALTY=9.244200
PRIMER_RIGHT_0_PENALTY=7.694441
PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT
PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=139,20
PRIMER_LEFT_0_TM=50.756
PRIMER_RIGHT_0_TM=52.306
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=35.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=59.4
=
