SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=0.032388
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_LEFT_1=609,20
PRIMER_RIGHT_1=868,20
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=260
PRIMER_PAIR_1_PRODUCT_TM=78.9
PRIMER_PAIR_2_PENALTY=0.037980
PRIMER_LEFT_2_PENALTY=0.031043
PRIMER_RIGHT_2_PENALTY=0.006937
PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_2=106,20
PRIMER_RIGHT_2=217,20
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
PRIMER_PAIR_3_PENALTY=0.052224
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt
PRIMER_LEFT_3=849,20
PRIMER_RIGHT_3=1040,20
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=192
PRIMER_PAIR_3_PRODUCT_TM=76.4
PRIMER_PAIR_4_PENALTY=0.064713
PRIMER_LEFT_4_PENALTY=0.006089
PRIMER_RIGHT_4_PENALTY=0.058624
PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg
PRIMER_LEFT_4=296,20
PRIMER_RIGHT_4=433,20
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=74.3
=
SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_END_GC=5
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=0.032388
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_LEFT_1=609,20
PRIMER_RIGHT_1=868,20
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=260
PRIMER_PAIR_1_PRODUCT_TM=78.9
PRIMER_PAIR_2_PENALTY=0.037980
PRIMER_LEFT_2_PENALTY=0.031043
PRIMER_RIGHT_2_PENALTY=0.006937
PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_2=106,20
PRIMER_RIGHT_2=217,20
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
PRIMER_PAIR_3_PENALTY=0.052224
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt
PRIMER_LEFT_3=849,20
PRIMER_RIGHT_3=1040,20
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=192
PRIMER_PAIR_3_PRODUCT_TM=76.4
PRIMER_PAIR_4_PENALTY=0.064713
PRIMER_LEFT_4_PENALTY=0.006089
PRIMER_RIGHT_4_PENALTY=0.058624
PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg
PRIMER_LEFT_4=296,20
PRIMER_RIGHT_4=433,20
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=74.3
=
SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_END_GC=4
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=0.032388
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_LEFT_1=609,20
PRIMER_RIGHT_1=868,20
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=260
PRIMER_PAIR_1_PRODUCT_TM=78.9
PRIMER_PAIR_2_PENALTY=0.037980
PRIMER_LEFT_2_PENALTY=0.031043
PRIMER_RIGHT_2_PENALTY=0.006937
PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_2=106,20
PRIMER_RIGHT_2=217,20
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
PRIMER_PAIR_3_PENALTY=0.052224
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt
PRIMER_LEFT_3=849,20
PRIMER_RIGHT_3=1040,20
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=192
PRIMER_PAIR_3_PRODUCT_TM=76.4
PRIMER_PAIR_4_PENALTY=0.064713
PRIMER_LEFT_4_PENALTY=0.006089
PRIMER_RIGHT_4_PENALTY=0.058624
PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg
PRIMER_LEFT_4=296,20
PRIMER_RIGHT_4=433,20
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=74.3
=
SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_END_GC=3
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=0.032388
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_LEFT_1=609,20
PRIMER_RIGHT_1=868,20
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=260
PRIMER_PAIR_1_PRODUCT_TM=78.9
PRIMER_PAIR_2_PENALTY=0.037980
PRIMER_LEFT_2_PENALTY=0.031043
PRIMER_RIGHT_2_PENALTY=0.006937
PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac
PRIMER_LEFT_2=106,20
PRIMER_RIGHT_2=217,20
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=112
PRIMER_PAIR_2_PRODUCT_TM=76.5
PRIMER_PAIR_3_PENALTY=0.052224
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt
PRIMER_LEFT_3=849,20
PRIMER_RIGHT_3=1040,20
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=192
PRIMER_PAIR_3_PRODUCT_TM=76.4
PRIMER_PAIR_4_PENALTY=0.064713
PRIMER_LEFT_4_PENALTY=0.006089
PRIMER_RIGHT_4_PENALTY=0.058624
PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc
PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg
PRIMER_LEFT_4=296,20
PRIMER_RIGHT_4=433,20
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=74.3
=
SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_END_GC=2
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.037132
PRIMER_LEFT_0_PENALTY=0.031043
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=106,20
PRIMER_RIGHT_0=315,20
PRIMER_LEFT_0_TM=59.969
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=210
PRIMER_PAIR_0_PRODUCT_TM=77.4
PRIMER_PAIR_1_PENALTY=0.052224
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=atcgccaccgtacaagaaac
PRIMER_RIGHT_1_SEQUENCE=cggtgcacggtaactaaggt
PRIMER_LEFT_1=849,20
PRIMER_RIGHT_1=1040,20
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=192
PRIMER_PAIR_1_PRODUCT_TM=76.4
PRIMER_PAIR_2_PENALTY=0.109692
PRIMER_LEFT_2_PENALTY=0.032351
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_LEFT_2_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_2_SEQUENCE=aatgcggttatccgaagatg
PRIMER_LEFT_2=609,20
PRIMER_RIGHT_2=820,20
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=59.923
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=212
PRIMER_PAIR_2_PRODUCT_TM=77.6
PRIMER_PAIR_3_PENALTY=0.154784
PRIMER_LEFT_3_PENALTY=0.051345
PRIMER_RIGHT_3_PENALTY=0.103439
PRIMER_LEFT_3_SEQUENCE=ttcattggaggctggttttc
PRIMER_RIGHT_3_SEQUENCE=cctcttcaccacccgttcta
PRIMER_LEFT_3=1184,20
PRIMER_RIGHT_3=1302,20
PRIMER_LEFT_3_TM=60.051
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=119
PRIMER_PAIR_3_PRODUCT_TM=76.9
PRIMER_PAIR_4_PENALTY=0.230339
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=0.152999
PRIMER_LEFT_4_SEQUENCE=catcttcggataaccgcatt
PRIMER_RIGHT_4_SEQUENCE=tcagcatgcagttgaaaacc
PRIMER_LEFT_4=801,20
PRIMER_RIGHT_4=1011,20
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=59.847
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=211
PRIMER_PAIR_4_PRODUCT_TM=77.7
=
SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_END_GC=1
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.154784
PRIMER_LEFT_0_PENALTY=0.051345
PRIMER_RIGHT_0_PENALTY=0.103439
PRIMER_LEFT_0_SEQUENCE=ttcattggaggctggttttc
PRIMER_RIGHT_0_SEQUENCE=cctcttcaccacccgttcta
PRIMER_LEFT_0=1184,20
PRIMER_RIGHT_0=1302,20
PRIMER_LEFT_0_TM=60.051
PRIMER_RIGHT_0_TM=60.103
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=119
PRIMER_PAIR_0_PRODUCT_TM=76.9
PRIMER_PAIR_1_PENALTY=0.373044
PRIMER_LEFT_1_PENALTY=0.277599
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=gggcctcattaatccaatca
PRIMER_RIGHT_1_SEQUENCE=aagcggcaaaggaggttaat
PRIMER_LEFT_1=757,20
PRIMER_RIGHT_1=897,20
PRIMER_LEFT_1_TM=59.722
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=141
PRIMER_PAIR_1_PRODUCT_TM=77.7
PRIMER_PAIR_2_PENALTY=0.436761
PRIMER_LEFT_2_PENALTY=0.385416
PRIMER_RIGHT_2_PENALTY=0.051345
PRIMER_LEFT_2_SEQUENCE=acggagcaaaaacgttgaat
PRIMER_RIGHT_2_SEQUENCE=gaaaaccagcctccaatgaa
PRIMER_LEFT_2=1063,20
PRIMER_RIGHT_2=1203,20
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=60.051
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=141
PRIMER_PAIR_2_PRODUCT_TM=72.8
PRIMER_PAIR_3_PENALTY=0.605909
PRIMER_LEFT_3_PENALTY=0.420643
PRIMER_RIGHT_3_PENALTY=0.185267
PRIMER_LEFT_3_SEQUENCE=agagacttgccgctcatttc
PRIMER_RIGHT_3_SEQUENCE=gcttcgatgatggcttgatt
PRIMER_LEFT_3=475,20
PRIMER_RIGHT_3=697,20
PRIMER_LEFT_3_TM=59.579
PRIMER_RIGHT_3_TM=60.185
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=223
PRIMER_PAIR_3_PRODUCT_TM=77.1
PRIMER_PAIR_4_PENALTY=0.821843
PRIMER_LEFT_4_PENALTY=0.815754
PRIMER_RIGHT_4_PENALTY=0.006089
PRIMER_LEFT_4_SEQUENCE=cacctcatggacaccatcat
PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_4=162,20
PRIMER_RIGHT_4=315,20
PRIMER_LEFT_4_TM=59.184
PRIMER_RIGHT_4_TM=60.006
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=154
PRIMER_PAIR_4_PRODUCT_TM=75.7
=
SEQUENCE_ID=Pick primers
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_END_GC=0
PRIMER_MIN_THREE_PRIME_DISTANCE=2
PRIMER_NUM_RETURN=5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.353221
PRIMER_LEFT_0_PENALTY=1.293240
PRIMER_RIGHT_0_PENALTY=1.059980
PRIMER_LEFT_0_SEQUENCE=tttccgtccttctgcattaaa
PRIMER_RIGHT_0_SEQUENCE=cagcctccaatgaatcaaaaa
PRIMER_LEFT_0=950,21
PRIMER_RIGHT_0=1197,21
PRIMER_LEFT_0_TM=59.707
PRIMER_RIGHT_0_TM=60.060
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=248
PRIMER_PAIR_0_PRODUCT_TM=75.0
PRIMER_PAIR_1_PENALTY=2.376287
PRIMER_LEFT_1_PENALTY=1.301938
PRIMER_RIGHT_1_PENALTY=1.074349
PRIMER_LEFT_1_SEQUENCE=tccctcggacaaccatttt
PRIMER_RIGHT_1_SEQUENCE=tttccacaaacccaccaataa
PRIMER_LEFT_1=897,19
PRIMER_RIGHT_1=1143,21
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=47.368
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=247
PRIMER_PAIR_1_PRODUCT_TM=75.1
PRIMER_PAIR_2_PENALTY=2.558875
PRIMER_LEFT_2_PENALTY=0.543622
PRIMER_RIGHT_2_PENALTY=2.015253
PRIMER_LEFT_2_SEQUENCE=tgatccggctcaaggaatta
PRIMER_RIGHT_2_SEQUENCE=tgttgtggtgatggtggttatt
PRIMER_LEFT_2=424,20
PRIMER_RIGHT_2=561,22
PRIMER_LEFT_2_TM=60.544
PRIMER_RIGHT_2_TM=60.015
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=138
PRIMER_PAIR_2_PRODUCT_TM=76.4
PRIMER_PAIR_3_PENALTY=3.451501
PRIMER_LEFT_3_PENALTY=1.482208
PRIMER_RIGHT_3_PENALTY=1.969292
PRIMER_LEFT_3_SEQUENCE=caacaacaacaacggcaataa
PRIMER_RIGHT_3_SEQUENCE=tcgagctaagtcacggttttt
PRIMER_LEFT_3=289,21
PRIMER_RIGHT_3=416,21
PRIMER_LEFT_3_TM=59.518
PRIMER_RIGHT_3_TM=59.031
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=128
PRIMER_PAIR_3_PRODUCT_TM=73.8
PRIMER_PAIR_4_PENALTY=3.999815
PRIMER_LEFT_4_PENALTY=2.675181
PRIMER_RIGHT_4_PENALTY=1.324634
PRIMER_LEFT_4_SEQUENCE=ccctacttccgacgaggattat
PRIMER_RIGHT_4_SEQUENCE=caccaacttcatgcgttttt
PRIMER_LEFT_4=68,22
PRIMER_RIGHT_4=327,20
PRIMER_LEFT_4_TM=60.675
PRIMER_RIGHT_4_TM=58.675
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=260
PRIMER_PAIR_4_PRODUCT_TM=78.1
=
