PRIMER PICKING RESULTS FOR 000

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   15.11   40.00  2.00  2.00 TCCTTGTGAT
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGAT
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 001

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   22.87   40.00  2.00  2.00 TCCTTGTGAT
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGAT
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 002

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   22.82   40.00  2.00  2.00 TCCTTGTGAT
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGAT
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 100

No mispriming library specified
Using 0-based sequence positions
NO LEFT PRIMER FOUND

    0 TCCTTGTGAt



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1     0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 101

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   22.87   40.00  2.00  2.00 TCCTTGTGaT
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGaT
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 102

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   22.82   40.00  2.00  2.00 tccttgtgat
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 tccttgtgat
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 010

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10    9.73   40.00  2.00  2.00 TCCTTGTGAT
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGAT
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 011

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   20.94   50.00  2.00  0.00 TCCTTGTGAG
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGAG
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 012

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   26.08   60.00  2.00  0.00 GCCTTGTGAG
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 GCCTTGTGAG
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 110

No mispriming library specified
Using 0-based sequence positions
NO LEFT PRIMER FOUND

    0 TCCTTGTGAt



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1     0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 111

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   18.40   40.00  2.00  2.00 TCCTTGTGaT
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 TCCTTGTGaT
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR 112

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   10   17.92   40.00  2.00  2.00 tccttgtgat
SEQUENCE SIZE: 10
INCLUDED REGION SIZE: 10

    0 tccttgtgat
      >>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR masking_001

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   20   46.14   40.00  3.00  3.00 GCCTTCATTAGAAAGTGACA
RIGHT PRIMER        69   20   46.49   40.00  4.00  1.00 ATTAGTACCCAGGTGTGATT
SEQUENCE SIZE: 70
INCLUDED REGION SIZE: 70

PRODUCT SIZE: 70, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT
      >>>>>>>>>>>>>>>>>>>>                              <<<<<<<<<<

   60 GGGTACTAAT
      <<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left      31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Right     31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR masking_002

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 70
INCLUDED REGION SIZE: 70


    0 GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNaATCACACCT
   60 GGGTACTAAT


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left      31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Right     31    30     0     0     0     0     0     0     0     0     0     0     0     1     0
Pair Stats:
considered 0, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR masking_003

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   20   46.14   40.00  3.00  3.00 GCCTTCATTAGAAAGTGACA
RIGHT PRIMER        69   20   46.49   40.00  4.00  1.00 aTTAGTACCCAGGTGTGATT
SEQUENCE SIZE: 70
INCLUDED REGION SIZE: 70

PRODUCT SIZE: 70, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT
      >>>>>>>>>>>>>>>>>>>>                              <<<<<<<<<<

   60 GGGTACTAAt
      <<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left      31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Right     31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR masking_004

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   20   46.14   40.00  3.00  3.00 gCCTTCATTAGAAAGTGACA
RIGHT PRIMER        69   20   46.49   40.00  4.00  1.00 ATTAGTACCCAGGTGTGATT
SEQUENCE SIZE: 70
INCLUDED REGION SIZE: 70

PRODUCT SIZE: 70, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 gCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT
      >>>>>>>>>>>>>>>>>>>>                              <<<<<<<<<<

   60 GGGTACTAAT
      <<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left      31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Right     31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR masking_005

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 70
INCLUDED REGION SIZE: 70


    0 GCCTTCATTAGAAAGTGACaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT
   60 GGGTACTAAT


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high lower      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end  case      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab   end  ok  
Left      31    30     0     0     0     0     0     0     0     0     0     0     0     1     0
Right     31    30     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
primer3 release 2.0.0


