SEQUENCE_ID=exon1
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
SEQUENCE_EXCLUDED_REGION=100,150
PRIMER_PRODUCT_SIZE_RANGE=200-250
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, low tm 2, high tm 399, ok 377
PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 702, ok 208
PRIMER_PAIR_EXPLAIN=considered 23, unacceptable product size 17, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.917137
PRIMER_LEFT_0_PENALTY=2.223427
PRIMER_RIGHT_0_PENALTY=3.693710
PRIMER_LEFT_0_SEQUENCE=TCACCCACGAGTGAAGAC
PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_0=43,18
PRIMER_RIGHT_0=279,18
PRIMER_LEFT_0_TM=55.223
PRIMER_RIGHT_0_TM=56.694
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=237
PRIMER_PAIR_0_PRODUCT_TM=82.8
PRIMER_PAIR_1_PENALTY=5.931775
PRIMER_LEFT_1_PENALTY=2.238065
PRIMER_RIGHT_1_PENALTY=3.693710
PRIMER_LEFT_1_SEQUENCE=GTTTCACCCACGAGTGAA
PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_1=40,18
PRIMER_RIGHT_1=279,18
PRIMER_LEFT_1_TM=55.238
PRIMER_RIGHT_1_TM=56.694
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=8.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=240
PRIMER_PAIR_1_PRODUCT_TM=82.7
PRIMER_PAIR_2_PENALTY=5.947634
PRIMER_LEFT_2_PENALTY=2.253924
PRIMER_RIGHT_2_PENALTY=3.693710
PRIMER_LEFT_2_SEQUENCE=AGTTTCACCCACGAGTGA
PRIMER_RIGHT_2_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_2=39,18
PRIMER_RIGHT_2=279,18
PRIMER_LEFT_2_TM=54.746
PRIMER_RIGHT_2_TM=56.694
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=241
PRIMER_PAIR_2_PRODUCT_TM=82.6
PRIMER_PAIR_3_PENALTY=5.947634
PRIMER_LEFT_3_PENALTY=2.253924
PRIMER_RIGHT_3_PENALTY=3.693710
PRIMER_LEFT_3_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_3=38,18
PRIMER_RIGHT_3=279,18
PRIMER_LEFT_3_TM=54.746
PRIMER_RIGHT_3_TM=56.694
PRIMER_LEFT_3_GC_PERCENT=55.556
PRIMER_RIGHT_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=242
PRIMER_PAIR_3_PRODUCT_TM=82.7
PRIMER_PAIR_4_PENALTY=6.090290
PRIMER_LEFT_4_PENALTY=2.223427
PRIMER_RIGHT_4_PENALTY=3.866863
PRIMER_LEFT_4_SEQUENCE=TCACCCACGAGTGAAGAC
PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC
PRIMER_LEFT_4=43,18
PRIMER_RIGHT_4=280,18
PRIMER_LEFT_4_TM=55.223
PRIMER_RIGHT_4_TM=56.867
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=238
PRIMER_PAIR_4_PRODUCT_TM=82.9
=
SEQUENCE_ID=exon2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 1, long poly-x seq 23, ok 507
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, ok 205
PRIMER_PAIR_EXPLAIN=considered 9, unacceptable product size 4, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.865499
PRIMER_LEFT_0_PENALTY=0.108160
PRIMER_RIGHT_0_PENALTY=3.757339
PRIMER_LEFT_0_SEQUENCE=CCCAAATCCCATTATTCATA
PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=267,23
PRIMER_LEFT_0_TM=54.892
PRIMER_RIGHT_0_TM=54.243
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=229
PRIMER_PAIR_0_PRODUCT_TM=73.9
PRIMER_PAIR_1_PENALTY=4.330804
PRIMER_LEFT_1_PENALTY=0.108160
PRIMER_RIGHT_1_PENALTY=4.222644
PRIMER_LEFT_1_SEQUENCE=CCCAAATCCCATTATTCATA
PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=268,24
PRIMER_LEFT_1_TM=54.892
PRIMER_RIGHT_1_TM=54.777
PRIMER_LEFT_1_GC_PERCENT=35.000
PRIMER_RIGHT_1_GC_PERCENT=29.167
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=230
PRIMER_PAIR_1_PRODUCT_TM=73.9
PRIMER_PAIR_2_PENALTY=4.567292
PRIMER_LEFT_2_PENALTY=0.809953
PRIMER_RIGHT_2_PENALTY=3.757339
PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=267,23
PRIMER_LEFT_2_TM=54.190
PRIMER_RIGHT_2_TM=54.243
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=247
PRIMER_PAIR_2_PRODUCT_TM=74.1
PRIMER_PAIR_3_PENALTY=4.567292
PRIMER_LEFT_3_PENALTY=0.809953
PRIMER_RIGHT_3_PENALTY=3.757339
PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=267,23
PRIMER_LEFT_3_TM=54.190
PRIMER_RIGHT_3_TM=54.243
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=30.435
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=248
PRIMER_PAIR_3_PRODUCT_TM=74.2
PRIMER_PAIR_4_PENALTY=4.944651
PRIMER_LEFT_4_PENALTY=1.187311
PRIMER_RIGHT_4_PENALTY=3.757339
PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_4=19,21
PRIMER_RIGHT_4=267,23
PRIMER_LEFT_4_TM=55.187
PRIMER_RIGHT_4_TM=54.243
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=30.435
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=249
PRIMER_PAIR_4_PRODUCT_TM=74.1
=
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_ID=exon2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 1, long poly-x seq 23, ok 507
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, ok 205
PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045
PRIMER_PAIR_EXPLAIN=considered 9, unacceptable product size 4, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.865499
PRIMER_LEFT_0_PENALTY=0.108160
PRIMER_RIGHT_0_PENALTY=3.757339
PRIMER_INTERNAL_0_PENALTY=0.055352
PRIMER_LEFT_0_SEQUENCE=CCCAAATCCCATTATTCATA
PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_0_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=267,23
PRIMER_INTERNAL_0=127,20
PRIMER_LEFT_0_TM=54.892
PRIMER_RIGHT_0_TM=54.243
PRIMER_INTERNAL_0_TM=59.945
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=229
PRIMER_PAIR_0_PRODUCT_TM=73.9
PRIMER_PAIR_1_PENALTY=4.330804
PRIMER_LEFT_1_PENALTY=0.108160
PRIMER_RIGHT_1_PENALTY=4.222644
PRIMER_INTERNAL_1_PENALTY=0.055352
PRIMER_LEFT_1_SEQUENCE=CCCAAATCCCATTATTCATA
PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_1_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=268,24
PRIMER_INTERNAL_1=127,20
PRIMER_LEFT_1_TM=54.892
PRIMER_RIGHT_1_TM=54.777
PRIMER_INTERNAL_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=35.000
PRIMER_RIGHT_1_GC_PERCENT=29.167
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=230
PRIMER_PAIR_1_PRODUCT_TM=73.9
PRIMER_PAIR_2_PENALTY=4.567292
PRIMER_LEFT_2_PENALTY=0.809953
PRIMER_RIGHT_2_PENALTY=3.757339
PRIMER_INTERNAL_2_PENALTY=0.055352
PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_2_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=267,23
PRIMER_INTERNAL_2=127,20
PRIMER_LEFT_2_TM=54.190
PRIMER_RIGHT_2_TM=54.243
PRIMER_INTERNAL_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=247
PRIMER_PAIR_2_PRODUCT_TM=74.1
PRIMER_PAIR_3_PENALTY=4.567292
PRIMER_LEFT_3_PENALTY=0.809953
PRIMER_RIGHT_3_PENALTY=3.757339
PRIMER_INTERNAL_3_PENALTY=0.055352
PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_3_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=267,23
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=54.190
PRIMER_RIGHT_3_TM=54.243
PRIMER_INTERNAL_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=30.435
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=248
PRIMER_PAIR_3_PRODUCT_TM=74.2
PRIMER_PAIR_4_PENALTY=4.944651
PRIMER_LEFT_4_PENALTY=1.187311
PRIMER_RIGHT_4_PENALTY=3.757339
PRIMER_INTERNAL_4_PENALTY=0.055352
PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_4_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_4=19,21
PRIMER_RIGHT_4=267,23
PRIMER_INTERNAL_4=127,20
PRIMER_LEFT_4_TM=55.187
PRIMER_RIGHT_4_TM=54.243
PRIMER_INTERNAL_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=30.435
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=249
PRIMER_PAIR_4_PRODUCT_TM=74.1
=
SEQUENCE_ID=exon1_2
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
SEQUENCE_EXCLUDED_REGION=100,150
PRIMER_PRODUCT_SIZE_RANGE=200-250
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16
PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, low tm 2, high tm 399, ok 377
PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 702, ok 208
PRIMER_INTERNAL_EXPLAIN=considered 3237, too many Ns 27, GC content failed 32, low tm 23, high tm 1880, ok 1275
PRIMER_PAIR_EXPLAIN=considered 26, unacceptable product size 17, high mispriming library similarity 2, ok 7
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.931775
PRIMER_LEFT_0_PENALTY=2.238065
PRIMER_RIGHT_0_PENALTY=3.693710
PRIMER_INTERNAL_0_PENALTY=0.010545
PRIMER_LEFT_0_SEQUENCE=GTTTCACCCACGAGTGAA
PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_INTERNAL_0_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
PRIMER_LEFT_0=40,18
PRIMER_RIGHT_0=279,18
PRIMER_INTERNAL_0=210,20
PRIMER_LEFT_0_TM=55.238
PRIMER_RIGHT_0_TM=56.694
PRIMER_INTERNAL_0_TM=59.989
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=240
PRIMER_PAIR_0_PRODUCT_TM=82.7
PRIMER_PAIR_1_PENALTY=5.947634
PRIMER_LEFT_1_PENALTY=2.253924
PRIMER_RIGHT_1_PENALTY=3.693710
PRIMER_INTERNAL_1_PENALTY=0.010545
PRIMER_LEFT_1_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_INTERNAL_1_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
PRIMER_LEFT_1=38,18
PRIMER_RIGHT_1=279,18
PRIMER_INTERNAL_1=210,20
PRIMER_LEFT_1_TM=54.746
PRIMER_RIGHT_1_TM=56.694
PRIMER_INTERNAL_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=55.556
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=242
PRIMER_PAIR_1_PRODUCT_TM=82.7
PRIMER_PAIR_2_PENALTY=6.090290
PRIMER_LEFT_2_PENALTY=2.223427
PRIMER_RIGHT_2_PENALTY=3.866863
PRIMER_INTERNAL_2_PENALTY=0.010545
PRIMER_LEFT_2_SEQUENCE=TCACCCACGAGTGAAGAC
PRIMER_RIGHT_2_SEQUENCE=GCTCCTCAGCCTTGTCAC
PRIMER_INTERNAL_2_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
PRIMER_LEFT_2=43,18
PRIMER_RIGHT_2=280,18
PRIMER_INTERNAL_2=210,20
PRIMER_LEFT_2_TM=55.223
PRIMER_RIGHT_2_TM=56.867
PRIMER_INTERNAL_2_TM=59.989
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=238
PRIMER_PAIR_2_PRODUCT_TM=82.9
PRIMER_PAIR_3_PENALTY=6.104928
PRIMER_LEFT_3_PENALTY=2.238065
PRIMER_RIGHT_3_PENALTY=3.866863
PRIMER_INTERNAL_3_PENALTY=0.010545
PRIMER_LEFT_3_SEQUENCE=GTTTCACCCACGAGTGAA
PRIMER_RIGHT_3_SEQUENCE=GCTCCTCAGCCTTGTCAC
PRIMER_INTERNAL_3_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
PRIMER_LEFT_3=40,18
PRIMER_RIGHT_3=280,18
PRIMER_INTERNAL_3=210,20
PRIMER_LEFT_3_TM=55.238
PRIMER_RIGHT_3_TM=56.867
PRIMER_INTERNAL_3_TM=59.989
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=61.111
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=241
PRIMER_PAIR_3_PRODUCT_TM=82.8
PRIMER_PAIR_4_PENALTY=6.120787
PRIMER_LEFT_4_PENALTY=2.253924
PRIMER_RIGHT_4_PENALTY=3.866863
PRIMER_INTERNAL_4_PENALTY=0.010545
PRIMER_LEFT_4_SEQUENCE=AGTTTCACCCACGAGTGA
PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC
PRIMER_INTERNAL_4_SEQUENCE=CTTCGAGTGGTCAAAGGAGC
PRIMER_LEFT_4=39,18
PRIMER_RIGHT_4=280,18
PRIMER_INTERNAL_4=210,20
PRIMER_LEFT_4_TM=54.746
PRIMER_RIGHT_4_TM=56.867
PRIMER_INTERNAL_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=242
PRIMER_PAIR_4_PRODUCT_TM=82.7
=
SEQUENCE_ID=exon2_2
PRIMER_NUM_RETURN=7
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 14, long poly-x seq 23, ok 494
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, high repeat similarity 19, ok 186
PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045
PRIMER_PAIR_EXPLAIN=considered 167, unacceptable product size 101, tm diff too large 12, high mispriming library similarity 46, ok 8
PRIMER_LEFT_NUM_RETURNED=7
PRIMER_RIGHT_NUM_RETURNED=7
PRIMER_INTERNAL_NUM_RETURNED=7
PRIMER_PAIR_NUM_RETURNED=7
PRIMER_PAIR_0_PENALTY=4.997478
PRIMER_LEFT_0_PENALTY=1.240139
PRIMER_RIGHT_0_PENALTY=3.757339
PRIMER_INTERNAL_0_PENALTY=0.055352
PRIMER_LEFT_0_SEQUENCE=TAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_0_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_0=23,19
PRIMER_RIGHT_0=267,23
PRIMER_INTERNAL_0=127,20
PRIMER_LEFT_0_TM=55.240
PRIMER_RIGHT_0_TM=54.243
PRIMER_INTERNAL_0_TM=59.945
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=245
PRIMER_PAIR_0_PRODUCT_TM=74.0
PRIMER_PAIR_1_PENALTY=5.462783
PRIMER_LEFT_1_PENALTY=1.240139
PRIMER_RIGHT_1_PENALTY=4.222644
PRIMER_INTERNAL_1_PENALTY=0.055352
PRIMER_LEFT_1_SEQUENCE=TAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_1_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_1=23,19
PRIMER_RIGHT_1=268,24
PRIMER_INTERNAL_1=127,20
PRIMER_LEFT_1_TM=55.240
PRIMER_RIGHT_1_TM=54.777
PRIMER_INTERNAL_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=47.368
PRIMER_RIGHT_1_GC_PERCENT=29.167
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=246
PRIMER_PAIR_1_PRODUCT_TM=74.0
PRIMER_PAIR_2_PENALTY=5.542868
PRIMER_LEFT_2_PENALTY=1.785529
PRIMER_RIGHT_2_PENALTY=3.757339
PRIMER_INTERNAL_2_PENALTY=0.055352
PRIMER_LEFT_2_SEQUENCE=TTAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_2_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_2=22,20
PRIMER_RIGHT_2=267,23
PRIMER_INTERNAL_2=127,20
PRIMER_LEFT_2_TM=56.786
PRIMER_RIGHT_2_TM=54.243
PRIMER_INTERNAL_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=246
PRIMER_PAIR_2_PRODUCT_TM=74.0
PRIMER_PAIR_3_PENALTY=5.790774
PRIMER_LEFT_3_PENALTY=2.033435
PRIMER_RIGHT_3_PENALTY=3.757339
PRIMER_INTERNAL_3_PENALTY=0.055352
PRIMER_LEFT_3_SEQUENCE=AAGTTTGTAACAGGGCCC
PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_3_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_3=24,18
PRIMER_RIGHT_3=267,23
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=55.033
PRIMER_RIGHT_3_TM=54.243
PRIMER_INTERNAL_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=30.435
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=244
PRIMER_PAIR_3_PRODUCT_TM=74.1
PRIMER_PAIR_4_PENALTY=6.008172
PRIMER_LEFT_4_PENALTY=1.785529
PRIMER_RIGHT_4_PENALTY=4.222644
PRIMER_INTERNAL_4_PENALTY=0.055352
PRIMER_LEFT_4_SEQUENCE=TTAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_4_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_4_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_4=22,20
PRIMER_RIGHT_4=268,24
PRIMER_INTERNAL_4=127,20
PRIMER_LEFT_4_TM=56.786
PRIMER_RIGHT_4_TM=54.777
PRIMER_INTERNAL_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=29.167
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=247
PRIMER_PAIR_4_PRODUCT_TM=73.9
PRIMER_PAIR_5_PENALTY=6.256078
PRIMER_LEFT_5_PENALTY=2.033435
PRIMER_RIGHT_5_PENALTY=4.222644
PRIMER_INTERNAL_5_PENALTY=0.055352
PRIMER_LEFT_5_SEQUENCE=AAGTTTGTAACAGGGCCC
PRIMER_RIGHT_5_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_5_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_5=24,18
PRIMER_RIGHT_5=268,24
PRIMER_INTERNAL_5=127,20
PRIMER_LEFT_5_TM=55.033
PRIMER_RIGHT_5_TM=54.777
PRIMER_INTERNAL_5_TM=59.945
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=29.167
PRIMER_INTERNAL_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_INTERNAL_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=6.00
PRIMER_RIGHT_5_SELF_END=6.00
PRIMER_INTERNAL_5_SELF_END=2.00
PRIMER_LEFT_5_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=12.4000
PRIMER_RIGHT_5_END_STABILITY=6.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=245
PRIMER_PAIR_5_PRODUCT_TM=74.0
PRIMER_PAIR_6_PENALTY=6.277288
PRIMER_LEFT_6_PENALTY=1.240139
PRIMER_RIGHT_6_PENALTY=5.037148
PRIMER_INTERNAL_6_PENALTY=0.055352
PRIMER_LEFT_6_SEQUENCE=TAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_6_SEQUENCE=TATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_6_SEQUENCE=GTTTGGCATGATTTTGCCTT
PRIMER_LEFT_6=23,19
PRIMER_RIGHT_6=269,25
PRIMER_INTERNAL_6=127,20
PRIMER_LEFT_6_TM=55.240
PRIMER_RIGHT_6_TM=54.963
PRIMER_INTERNAL_6_TM=59.945
PRIMER_LEFT_6_GC_PERCENT=47.368
PRIMER_RIGHT_6_GC_PERCENT=28.000
PRIMER_INTERNAL_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_INTERNAL_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=6.00
PRIMER_RIGHT_6_SELF_END=6.00
PRIMER_INTERNAL_6_SELF_END=2.00
PRIMER_LEFT_6_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=12.4000
PRIMER_RIGHT_6_END_STABILITY=6.0000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=247
PRIMER_PAIR_6_PRODUCT_TM=73.9
=
PRIMER_NUM_RETURN=5
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_ID=exon2_3
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 124, long poly-x seq 23, ok 384
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, high repeat similarity 176, ok 29
PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045
PRIMER_PAIR_EXPLAIN=considered 12459, unacceptable product size 6872, tm diff too large 4075, high mispriming library similarity 1512, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
