SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 1 any right primer - OK
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=40-100
PRIMER_MIN_GC=0
PRIMER_MIN_TM=0
PRIMER_PICK_ANYWAY=0
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_POLY_X=100
PRIMER_MAX_SELF_ANY=100
PRIMER_MAX_SELF_END=100
PRIMER_FIRST_BASE_INDEX=1
PRIMER_MIN_SIZE=16
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAga
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 2 any left primer - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,17
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 3 any pair - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccA
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 4 constrained left and right primers - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 5 constrained left and right primers - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,10
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 6 constrained left and right primers - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,7 ; 1,25,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 7 contrained left and right primers - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,19,49,17 ; 1,20,50,16
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 8 any left primer - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,16
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 9 any right primer - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 10 any right primer, any left primer - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,, ; ,,49,17
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
=
