PRIMER_MAX_SELF_ANY=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_END=8
P3_FILE_FLAG=1
SEQUENCE_ID=targets_test
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
SEQUENCE_TARGET=21,122
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=3.432274
PRIMER_LEFT_0_PENALTY=1.117633
PRIMER_RIGHT_0_PENALTY=2.314641
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_0=0,21
PRIMER_RIGHT_0=160,18
PRIMER_LEFT_0_TM=60.118
PRIMER_RIGHT_0_TM=59.685
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=12.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=161
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=4.086374
PRIMER_LEFT_1_PENALTY=1.771733
PRIMER_RIGHT_1_PENALTY=2.314641
PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=160,18
PRIMER_LEFT_1_TM=58.228
PRIMER_RIGHT_1_TM=59.685
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=160
PRIMER_PAIR_1_PRODUCT_TM=74.3
PRIMER_PAIR_2_PENALTY=5.046339
PRIMER_LEFT_2_PENALTY=2.731698
PRIMER_RIGHT_2_PENALTY=2.314641
PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=160,18
PRIMER_LEFT_2_TM=57.268
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=161
PRIMER_PAIR_2_PRODUCT_TM=74.3
=
SEQUENCE_ID=SP01-02.txt
SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.334195
PRIMER_LEFT_0_PENALTY=2.101896
PRIMER_RIGHT_0_PENALTY=0.232299
PRIMER_LEFT_0_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_0_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT_0=9,22
PRIMER_RIGHT_0=247,20
PRIMER_LEFT_0_TM=59.898
PRIMER_RIGHT_0_TM=60.232
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=239
PRIMER_PAIR_0_PRODUCT_TM=75.7
PRIMER_PAIR_1_PENALTY=2.773493
PRIMER_LEFT_1_PENALTY=2.101896
PRIMER_RIGHT_1_PENALTY=0.671597
PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=246,20
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.328
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=238
PRIMER_PAIR_1_PRODUCT_TM=75.8
PRIMER_PAIR_2_PENALTY=2.846821
PRIMER_LEFT_2_PENALTY=2.101896
PRIMER_RIGHT_2_PENALTY=0.744925
PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=248,20
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.745
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=240
PRIMER_PAIR_2_PRODUCT_TM=75.7
PRIMER_PAIR_3_PENALTY=3.098430
PRIMER_LEFT_3_PENALTY=2.866131
PRIMER_RIGHT_3_PENALTY=0.232299
PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT_3=11,21
PRIMER_RIGHT_3=247,20
PRIMER_LEFT_3_TM=58.134
PRIMER_RIGHT_3_TM=60.232
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=237
PRIMER_PAIR_3_PRODUCT_TM=75.7
PRIMER_PAIR_4_PENALTY=3.235559
PRIMER_LEFT_4_PENALTY=3.003259
PRIMER_RIGHT_4_PENALTY=0.232299
PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=247,20
PRIMER_LEFT_4_TM=58.997
PRIMER_RIGHT_4_TM=60.232
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=238
PRIMER_PAIR_4_PRODUCT_TM=75.8
=
SEQUENCE_ID=SP01-03.txt
SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.785028
PRIMER_LEFT_0_PENALTY=0.040772
PRIMER_RIGHT_0_PENALTY=0.744257
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_RIGHT_0_SEQUENCE=ATCCTGCTGAAGCAAATGGA
PRIMER_LEFT_0=72,20
PRIMER_RIGHT_0=173,20
PRIMER_LEFT_0_TM=59.959
PRIMER_RIGHT_0_TM=60.744
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=102
PRIMER_PAIR_0_PRODUCT_TM=78.9
PRIMER_PAIR_1_PENALTY=0.785028
PRIMER_LEFT_1_PENALTY=0.040772
PRIMER_RIGHT_1_PENALTY=0.744257
PRIMER_LEFT_1_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_RIGHT_1_SEQUENCE=GATCCTGCTGAAGCAAATGG
PRIMER_LEFT_1=72,20
PRIMER_RIGHT_1=174,20
PRIMER_LEFT_1_TM=59.959
PRIMER_RIGHT_1_TM=60.744
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=103
PRIMER_PAIR_1_PRODUCT_TM=79.2
PRIMER_PAIR_2_PENALTY=1.015937
PRIMER_LEFT_2_PENALTY=0.271680
PRIMER_RIGHT_2_PENALTY=0.744257
PRIMER_LEFT_2_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_RIGHT_2_SEQUENCE=ATCCTGCTGAAGCAAATGGA
PRIMER_LEFT_2=64,20
PRIMER_RIGHT_2=173,20
PRIMER_LEFT_2_TM=59.728
PRIMER_RIGHT_2_TM=60.744
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=110
PRIMER_PAIR_2_PRODUCT_TM=79.4
PRIMER_PAIR_3_PENALTY=1.015937
PRIMER_LEFT_3_PENALTY=0.271680
PRIMER_RIGHT_3_PENALTY=0.744257
PRIMER_LEFT_3_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_RIGHT_3_SEQUENCE=GATCCTGCTGAAGCAAATGG
PRIMER_LEFT_3=64,20
PRIMER_RIGHT_3=174,20
PRIMER_LEFT_3_TM=59.728
PRIMER_RIGHT_3_TM=60.744
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=111
PRIMER_PAIR_3_PRODUCT_TM=79.6
PRIMER_PAIR_4_PENALTY=1.015937
PRIMER_LEFT_4_PENALTY=0.271680
PRIMER_RIGHT_4_PENALTY=0.744257
PRIMER_LEFT_4_SEQUENCE=CACCCTGCAGGTTTAGAAGG
PRIMER_RIGHT_4_SEQUENCE=ATCCTGCTGAAGCAAATGGA
PRIMER_LEFT_4=60,20
PRIMER_RIGHT_4=173,20
PRIMER_LEFT_4_TM=59.728
PRIMER_RIGHT_4_TM=60.744
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=114
PRIMER_PAIR_4_PRODUCT_TM=79.8
=
SEQUENCE_ID=SP01-06.txt
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.738411
PRIMER_LEFT_0_PENALTY=4.596160
PRIMER_RIGHT_0_PENALTY=2.142251
PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_0=30,24
PRIMER_RIGHT_0=129,18
PRIMER_LEFT_0_TM=59.404
PRIMER_RIGHT_0_TM=60.142
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_0_PRODUCT_TM=69.9
PRIMER_PAIR_1_PENALTY=7.148392
PRIMER_LEFT_1_PENALTY=5.006141
PRIMER_RIGHT_1_PENALTY=2.142251
PRIMER_LEFT_1_SEQUENCE=TTATGAATCGCAATCAAATTTTTT
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_1=29,24
PRIMER_RIGHT_1=129,18
PRIMER_LEFT_1_TM=58.994
PRIMER_RIGHT_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=20.833
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=101
PRIMER_PAIR_1_PRODUCT_TM=69.8
PRIMER_PAIR_2_PENALTY=7.344055
PRIMER_LEFT_2_PENALTY=5.201804
PRIMER_RIGHT_2_PENALTY=2.142251
PRIMER_LEFT_2_SEQUENCE=TATGAATCGCAATCAAATTTTTTCT
PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_2=30,25
PRIMER_RIGHT_2=129,18
PRIMER_LEFT_2_TM=60.202
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=24.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_2_PRODUCT_TM=69.9
PRIMER_PAIR_3_PENALTY=7.346874
PRIMER_LEFT_3_PENALTY=5.204623
PRIMER_RIGHT_3_PENALTY=2.142251
PRIMER_LEFT_3_SEQUENCE=TATGAATCGCAATCAAATTTTTT
PRIMER_RIGHT_3_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_3=30,23
PRIMER_RIGHT_3=129,18
PRIMER_LEFT_3_TM=57.795
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=21.739
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_3_PRODUCT_TM=69.9
PRIMER_PAIR_4_PENALTY=7.346874
PRIMER_LEFT_4_PENALTY=5.204623
PRIMER_RIGHT_4_PENALTY=2.142251
PRIMER_LEFT_4_SEQUENCE=TTATGAATCGCAATCAAATTTTT
PRIMER_RIGHT_4_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_4=29,23
PRIMER_RIGHT_4=129,18
PRIMER_LEFT_4_TM=57.795
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=21.739
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=101
PRIMER_PAIR_4_PRODUCT_TM=69.8
=
SEQUENCE_ID=SP01-15.txt
SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SP01-19.txt
SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.269854
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.196903
PRIMER_LEFT_0_SEQUENCE=TATTCCTGTCCCTGTGCCTC
PRIMER_RIGHT_0_SEQUENCE=CCCTCATCCAGTGCTCTTTC
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=183,20
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=59.803
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=136
PRIMER_PAIR_0_PRODUCT_TM=76.0
PRIMER_PAIR_1_PENALTY=0.269854
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.196903
PRIMER_LEFT_1_SEQUENCE=TATTCCTGTCCCTGTGCCTC
PRIMER_RIGHT_1_SEQUENCE=TCCCTCATCCAGTGCTCTTT
PRIMER_LEFT_1=48,20
PRIMER_RIGHT_1=184,20
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=137
PRIMER_PAIR_1_PRODUCT_TM=75.9
PRIMER_PAIR_2_PENALTY=0.303362
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=0.230410
PRIMER_LEFT_2_SEQUENCE=TATTCCTGTCCCTGTGCCTC
PRIMER_RIGHT_2_SEQUENCE=CAGTGCTCTTTCTGCCAGTG
PRIMER_LEFT_2=48,20
PRIMER_RIGHT_2=175,20
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=59.770
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=128
PRIMER_PAIR_2_PRODUCT_TM=75.4
PRIMER_PAIR_3_PENALTY=0.593847
PRIMER_LEFT_3_PENALTY=0.396944
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_LEFT_3_SEQUENCE=GCCTCAGAAACCTGGCTACA
PRIMER_RIGHT_3_SEQUENCE=CCCTCATCCAGTGCTCTTTC
PRIMER_LEFT_3=63,20
PRIMER_RIGHT_3=183,20
PRIMER_LEFT_3_TM=60.397
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=121
PRIMER_PAIR_3_PRODUCT_TM=75.6
PRIMER_PAIR_4_PENALTY=0.593847
PRIMER_LEFT_4_PENALTY=0.396944
PRIMER_RIGHT_4_PENALTY=0.196903
PRIMER_LEFT_4_SEQUENCE=GCCTCAGAAACCTGGCTACA
PRIMER_RIGHT_4_SEQUENCE=TCCCTCATCCAGTGCTCTTT
PRIMER_LEFT_4=63,20
PRIMER_RIGHT_4=184,20
PRIMER_LEFT_4_TM=60.397
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=122
PRIMER_PAIR_4_PRODUCT_TM=75.5
=
SEQUENCE_ID=SP01-20.txt
SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SP01-21.txt
SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.103491
PRIMER_LEFT_0_PENALTY=2.224770
PRIMER_RIGHT_0_PENALTY=2.878720
PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=155,22
PRIMER_LEFT_0_TM=57.775
PRIMER_RIGHT_0_TM=59.121
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=156
PRIMER_PAIR_0_PRODUCT_TM=72.1
PRIMER_PAIR_1_PENALTY=5.103491
PRIMER_LEFT_1_PENALTY=2.224770
PRIMER_RIGHT_1_PENALTY=2.878720
PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=164,22
PRIMER_LEFT_1_TM=57.775
PRIMER_RIGHT_1_TM=59.121
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=72.4
PRIMER_PAIR_2_PENALTY=5.103491
PRIMER_LEFT_2_PENALTY=2.224770
PRIMER_RIGHT_2_PENALTY=2.878720
PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=166,22
PRIMER_LEFT_2_TM=57.775
PRIMER_RIGHT_2_TM=59.121
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=167
PRIMER_PAIR_2_PRODUCT_TM=72.5
PRIMER_PAIR_3_PENALTY=5.288748
PRIMER_LEFT_3_PENALTY=2.224770
PRIMER_RIGHT_3_PENALTY=3.063978
PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=156,23
PRIMER_LEFT_3_TM=57.775
PRIMER_RIGHT_3_TM=60.064
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=72.0
PRIMER_PAIR_4_PENALTY=5.288748
PRIMER_LEFT_4_PENALTY=2.224770
PRIMER_RIGHT_4_PENALTY=3.063978
PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=163,23
PRIMER_LEFT_4_TM=57.775
PRIMER_RIGHT_4_TM=60.064
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=7.00
PRIMER_PAIR_4_PRODUCT_SIZE=164
PRIMER_PAIR_4_PRODUCT_TM=72.2
=
SEQUENCE_ID=SP03-40.txt
SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=SP04-07.txt
SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.103348
PRIMER_LEFT_0_PENALTY=0.051674
PRIMER_RIGHT_0_PENALTY=0.051674
PRIMER_LEFT_0_SEQUENCE=CCCTACATACAGCCAAGGGA
PRIMER_RIGHT_0_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_0=21,20
PRIMER_RIGHT_0=281,20
PRIMER_LEFT_0_TM=59.948
PRIMER_RIGHT_0_TM=59.948
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=261
PRIMER_PAIR_0_PRODUCT_TM=78.3
PRIMER_PAIR_1_PENALTY=0.103348
PRIMER_LEFT_1_PENALTY=0.051674
PRIMER_RIGHT_1_PENALTY=0.051674
PRIMER_LEFT_1_SEQUENCE=TCCCTACATACAGCCAAGGG
PRIMER_RIGHT_1_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_1=20,20
PRIMER_RIGHT_1=281,20
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=262
PRIMER_PAIR_1_PRODUCT_TM=78.3
PRIMER_PAIR_2_PENALTY=0.117997
PRIMER_LEFT_2_PENALTY=0.066323
PRIMER_RIGHT_2_PENALTY=0.051674
PRIMER_LEFT_2_SEQUENCE=CTAAGAATGTGGCCAAGGGA
PRIMER_RIGHT_2_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_2=122,20
PRIMER_RIGHT_2=281,20
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=160
PRIMER_PAIR_2_PRODUCT_TM=76.4
PRIMER_PAIR_3_PENALTY=0.117997
PRIMER_LEFT_3_PENALTY=0.066323
PRIMER_RIGHT_3_PENALTY=0.051674
PRIMER_LEFT_3_SEQUENCE=TCCCTAAGAATGTGGCCAAG
PRIMER_RIGHT_3_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_3=119,20
PRIMER_RIGHT_3=281,20
PRIMER_LEFT_3_TM=60.066
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=163
PRIMER_PAIR_3_PRODUCT_TM=76.6
PRIMER_PAIR_4_PENALTY=0.117997
PRIMER_LEFT_4_PENALTY=0.051674
PRIMER_RIGHT_4_PENALTY=0.066323
PRIMER_LEFT_4_SEQUENCE=CCCTACATACAGCCAAGGGA
PRIMER_RIGHT_4_SEQUENCE=CTTGGCCACATTCTTAGGGA
PRIMER_LEFT_4=21,20
PRIMER_RIGHT_4=138,20
PRIMER_LEFT_4_TM=59.948
PRIMER_RIGHT_4_TM=60.066
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=118
PRIMER_PAIR_4_PRODUCT_TM=76.0
=
SEQUENCE_ID=AGAG
SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=long_seq
SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.361544
PRIMER_LEFT_0_PENALTY=0.221791
PRIMER_RIGHT_0_PENALTY=0.139753
PRIMER_LEFT_0_SEQUENCE=gatcgatgcatgcatgctac
PRIMER_RIGHT_0_SEQUENCE=cccagctagctagcatcgtc
PRIMER_LEFT_0=445,20
PRIMER_RIGHT_0=676,20
PRIMER_LEFT_0_TM=60.222
PRIMER_RIGHT_0_TM=60.140
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=10.00
PRIMER_RIGHT_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=232
PRIMER_PAIR_0_PRODUCT_TM=77.8
PRIMER_PAIR_1_PENALTY=0.398026
PRIMER_LEFT_1_PENALTY=0.258273
PRIMER_RIGHT_1_PENALTY=0.139753
PRIMER_LEFT_1_SEQUENCE=gatgctagctaggcgatgct
PRIMER_RIGHT_1_SEQUENCE=cccagctagctagcatcgtc
PRIMER_LEFT_1=408,20
PRIMER_RIGHT_1=676,20
PRIMER_LEFT_1_TM=59.742
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=12.00
PRIMER_PAIR_1_COMPL_END=7.00
PRIMER_PAIR_1_PRODUCT_SIZE=269
PRIMER_PAIR_1_PRODUCT_TM=78.1
PRIMER_PAIR_2_PENALTY=0.405097
PRIMER_LEFT_2_PENALTY=0.120169
PRIMER_RIGHT_2_PENALTY=0.284927
PRIMER_LEFT_2_SEQUENCE=gcgatcgatgctagctagct
PRIMER_RIGHT_2_SEQUENCE=ctagctagatcgccgctacg
PRIMER_LEFT_2=372,20
PRIMER_RIGHT_2=595,20
PRIMER_LEFT_2_TM=59.880
PRIMER_RIGHT_2_TM=60.285
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=10.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=224
PRIMER_PAIR_2_PRODUCT_TM=77.5
PRIMER_PAIR_3_PENALTY=0.500121
PRIMER_LEFT_3_PENALTY=0.221791
PRIMER_RIGHT_3_PENALTY=0.278329
PRIMER_LEFT_3_SEQUENCE=gatcgatgcatgcatgctac
PRIMER_RIGHT_3_SEQUENCE=gtcagctagctagatcgccg
PRIMER_LEFT_3=445,20
PRIMER_RIGHT_3=600,20
PRIMER_LEFT_3_TM=60.222
PRIMER_RIGHT_3_TM=60.278
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=13.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=156
PRIMER_PAIR_3_PRODUCT_TM=76.3
PRIMER_PAIR_4_PENALTY=0.506718
PRIMER_LEFT_4_PENALTY=0.221791
PRIMER_RIGHT_4_PENALTY=0.284927
PRIMER_LEFT_4_SEQUENCE=gatcgatgcatgcatgctac
PRIMER_RIGHT_4_SEQUENCE=ctagctagatcgccgctacg
PRIMER_LEFT_4=445,20
PRIMER_RIGHT_4=595,20
PRIMER_LEFT_4_TM=60.222
PRIMER_RIGHT_4_TM=60.285
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=10.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=8.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=151
PRIMER_PAIR_4_PRODUCT_TM=76.0
=
SEQUENCE_ID=bad_letter
SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
PRIMER_ERROR=Unrecognized base in input sequence
=
SEQUENCE_ID=bad_target
SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
SEQUENCE_TARGET=50,10
SEQUENCE_TARGET=500,50
PRIMER_ERROR=TARGET beyond end of sequence
=
SEQUENCE_ID=other_bad_target
SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
SEQUENCE_TARGET=100,1000
PRIMER_ERROR=TARGET beyond end of sequence
=
SEQUENCE_ID=included_test
SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
SEQUENCE_INCLUDED_REGION=20,228
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.103491
PRIMER_LEFT_0_PENALTY=2.224770
PRIMER_RIGHT_0_PENALTY=2.878720
PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA
PRIMER_LEFT_0=20,20
PRIMER_RIGHT_0=175,22
PRIMER_LEFT_0_TM=57.775
PRIMER_RIGHT_0_TM=59.121
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=156
PRIMER_PAIR_0_PRODUCT_TM=72.1
PRIMER_PAIR_1_PENALTY=5.103491
PRIMER_LEFT_1_PENALTY=2.224770
PRIMER_RIGHT_1_PENALTY=2.878720
PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA
PRIMER_LEFT_1=20,20
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=57.775
PRIMER_RIGHT_1_TM=59.121
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=72.4
PRIMER_PAIR_2_PENALTY=5.103491
PRIMER_LEFT_2_PENALTY=2.224770
PRIMER_RIGHT_2_PENALTY=2.878720
PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA
PRIMER_LEFT_2=20,20
PRIMER_RIGHT_2=186,22
PRIMER_LEFT_2_TM=57.775
PRIMER_RIGHT_2_TM=59.121
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=167
PRIMER_PAIR_2_PRODUCT_TM=72.5
PRIMER_PAIR_3_PENALTY=5.288748
PRIMER_LEFT_3_PENALTY=2.224770
PRIMER_RIGHT_3_PENALTY=3.063978
PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=176,23
PRIMER_LEFT_3_TM=57.775
PRIMER_RIGHT_3_TM=60.064
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=72.0
PRIMER_PAIR_4_PENALTY=5.288748
PRIMER_LEFT_4_PENALTY=2.224770
PRIMER_RIGHT_4_PENALTY=3.063978
PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA
PRIMER_LEFT_4=20,20
PRIMER_RIGHT_4=183,23
PRIMER_LEFT_4_TM=57.775
PRIMER_RIGHT_4_TM=60.064
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=7.00
PRIMER_PAIR_4_PRODUCT_SIZE=164
PRIMER_PAIR_4_PRODUCT_TM=72.2
=
SEQUENCE_ID=included_test1
SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
SEQUENCE_INCLUDED_REGION=20-200
PRIMER_ERROR=Illegal SEQUENCE_INCLUDED_REGION value: 20-200
=
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MAX_SELF_END=8
SEQUENCE_ID=included_test_w_middle
SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
SEQUENCE_INCLUDED_REGION=20,228
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.103491
PRIMER_LEFT_0_PENALTY=2.224770
PRIMER_RIGHT_0_PENALTY=2.878720
PRIMER_INTERNAL_0_PENALTY=1.680010
PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA
PRIMER_INTERNAL_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_0=20,20
PRIMER_RIGHT_0=175,22
PRIMER_INTERNAL_0=78,21
PRIMER_LEFT_0_TM=57.775
PRIMER_RIGHT_0_TM=59.121
PRIMER_INTERNAL_0_TM=60.680
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=156
PRIMER_PAIR_0_PRODUCT_TM=72.1
PRIMER_PAIR_1_PENALTY=5.103491
PRIMER_LEFT_1_PENALTY=2.224770
PRIMER_RIGHT_1_PENALTY=2.878720
PRIMER_INTERNAL_1_PENALTY=1.680010
PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA
PRIMER_INTERNAL_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_1=20,20
PRIMER_RIGHT_1=184,22
PRIMER_INTERNAL_1=78,21
PRIMER_LEFT_1_TM=57.775
PRIMER_RIGHT_1_TM=59.121
PRIMER_INTERNAL_1_TM=60.680
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_INTERNAL_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=72.4
PRIMER_PAIR_2_PENALTY=5.103491
PRIMER_LEFT_2_PENALTY=2.224770
PRIMER_RIGHT_2_PENALTY=2.878720
PRIMER_INTERNAL_2_PENALTY=1.680010
PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA
PRIMER_INTERNAL_2_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_2=20,20
PRIMER_RIGHT_2=186,22
PRIMER_INTERNAL_2=78,21
PRIMER_LEFT_2_TM=57.775
PRIMER_RIGHT_2_TM=59.121
PRIMER_INTERNAL_2_TM=60.680
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_INTERNAL_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=167
PRIMER_PAIR_2_PRODUCT_TM=72.5
PRIMER_PAIR_3_PENALTY=5.288748
PRIMER_LEFT_3_PENALTY=2.224770
PRIMER_RIGHT_3_PENALTY=3.063978
PRIMER_INTERNAL_3_PENALTY=1.680010
PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA
PRIMER_INTERNAL_3_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=176,23
PRIMER_INTERNAL_3=78,21
PRIMER_LEFT_3_TM=57.775
PRIMER_RIGHT_3_TM=60.064
PRIMER_INTERNAL_3_TM=60.680
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=72.0
PRIMER_PAIR_4_PENALTY=5.288748
PRIMER_LEFT_4_PENALTY=2.224770
PRIMER_RIGHT_4_PENALTY=3.063978
PRIMER_INTERNAL_4_PENALTY=1.680010
PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA
PRIMER_INTERNAL_4_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_4=20,20
PRIMER_RIGHT_4=183,23
PRIMER_INTERNAL_4=78,21
PRIMER_LEFT_4_TM=57.775
PRIMER_RIGHT_4_TM=60.064
PRIMER_INTERNAL_4_TM=60.680
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_INTERNAL_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=7.00
PRIMER_PAIR_4_PRODUCT_SIZE=164
PRIMER_PAIR_4_PRODUCT_TM=72.2
=
SEQUENCE_ID=long_primer_lists
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
P3_FILE_FLAG=0
PRIMER_MIN_TM=0
PRIMER_MAX_TM=100
PRIMER_MAX_SELF_ANY=20
PRIMER_MAX_SELF_END=20
PRIMER_PAIR_MAX_COMPL_ANY=20
PRIMER_PAIR_MAX_COMPL_END=20
SEQUENCE_TEMPLATE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCGTTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCTGCAATAAACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATCTACCCAGTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATTCCTGAGGAATTGCCACACTGACTTCCGCAATGGTTGAACTAGTTAATTAGATCCCGTTTGTCC
PRIMER_LEFT_EXPLAIN=considered 5210, GC content failed 120, ok 5090
PRIMER_RIGHT_EXPLAIN=considered 5210, GC content failed 24, ok 5186
PRIMER_INTERNAL_EXPLAIN=considered 5985, GC content failed 120, low tm 2448, high tm 1678, ok 1739
PRIMER_PAIR_EXPLAIN=considered 7, unacceptable product size 6, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.316568
PRIMER_LEFT_0_PENALTY=0.278124
PRIMER_RIGHT_0_PENALTY=0.038444
PRIMER_INTERNAL_0_PENALTY=0.609876
PRIMER_LEFT_0_SEQUENCE=TGTGCTGCACCCATTAACTC
PRIMER_RIGHT_0_SEQUENCE=AGGAACATCACACTCTGGGG
PRIMER_INTERNAL_0_SEQUENCE=CTAGTGCTATCCCTGCCCCT
PRIMER_LEFT_0=84,20
PRIMER_RIGHT_0=187,20
PRIMER_INTERNAL_0=128,20
PRIMER_LEFT_0_TM=59.722
PRIMER_RIGHT_0_TM=59.962
PRIMER_INTERNAL_0_TM=60.610
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=104
PRIMER_PAIR_0_PRODUCT_TM=75.4
=
SEQUENCE_ID=end_complementarity_failure
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_TM=0
PRIMER_MAX_TM=100
PRIMER_MAX_SELF_ANY=20
PRIMER_MAX_SELF_END=2
PRIMER_PAIR_MAX_COMPL_ANY=20
PRIMER_PAIR_MAX_COMPL_END=2
PRIMER_INTERNAL_MAX_SELF_END=2
SEQUENCE_TEMPLATE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCC
PRIMER_LEFT_EXPLAIN=considered 2740, GC content failed 120, ok 2620
PRIMER_RIGHT_EXPLAIN=considered 2740, GC content failed 24, ok 2716
PRIMER_INTERNAL_EXPLAIN=considered 3515, GC content failed 120, low tm 1357, high tm 1080, high end compl 1, ok 957
PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.316568
PRIMER_LEFT_0_PENALTY=0.278124
PRIMER_RIGHT_0_PENALTY=0.038444
PRIMER_INTERNAL_0_PENALTY=0.609876
PRIMER_LEFT_0_SEQUENCE=TGTGCTGCACCCATTAACTC
PRIMER_RIGHT_0_SEQUENCE=AGGAACATCACACTCTGGGG
PRIMER_INTERNAL_0_SEQUENCE=CTAGTGCTATCCCTGCCCCT
PRIMER_LEFT_0=84,20
PRIMER_RIGHT_0=187,20
PRIMER_INTERNAL_0=128,20
PRIMER_LEFT_0_TM=59.722
PRIMER_RIGHT_0_TM=59.962
PRIMER_INTERNAL_0_TM=60.610
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=104
PRIMER_PAIR_0_PRODUCT_TM=75.4
=
SEQUENCE_TEMPLATE=GATCGATCGATGCTAGCTGACTGATCGTAGCTAGTCGATCGTAGCTAGCTATCGTACTACTATCATCTAGCTACTGATCGATGGGGCTGTGCTTCGATGCGGTGACGGCTAGCTAGCTGATCGGCTAGCTGACGCTGATCGATGCTATACTACATCTACTACTACGATCACTGGGCATCGTAGCTAGCTACACATCTAGCTGACAGC
P3_FILE_FLAG=1
PRIMER_ERROR=Need PRIMER_SEQUENCE_ID if PRIMER_FILE_FLAG is not 0
=
SEQUENCE_ID=bad_included_region
SEQUENCE_TEMPLATE=ATCTGCTAGCTGATCGAGCTATCATGCTAGCTGATCATGCTAGCTATCGTACTACTATCATCTACTGATCATGCTAGCTACTATCATCTACTATCATCATCTAGCTACTAGCTACTACGATCTACTACTATCATCTACTAGCTACTATCACTACTACTATCATCATCTACTACTACATCACACTGATCGTGGGCTAGCTGGCAGCGCG
SEQUENCE_INCLUDED_REGION=-1,100
P3_FILE_FLAG=0
PRIMER_ERROR=Illegal value for SEQUENCE_INCLUDED_REGION
=
SEQUENCE_ID=bad_included_region1
SEQUENCE_TEMPLATE=GTAGCAGCTAGCTAGCTAGTCGATCGTAGCTAGCTAGTCATCTACGTAGCTAGCTAGTCATCGTAGCTGATCGATGCTAGCTAGCTGATCGGGCGACTGTTCATATGATGGGCTACTGACTGATCGTAGCTGGCGATCGTAGCGAGGCGATCTAGCTAGCTAGCTATCGGCTACTGATCGTACTGACTGATGCTAGCTAGGGCGTAGCTAG
SEQUENCE_INCLUDED_REGION=150,-90
PRIMER_ERROR=Illegal value for SEQUENCE_INCLUDED_REGION
=
SEQUENCE_ID=SP01-06-with-repeat1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib
PRIMER_MAX_LIBRARY_MISPRIMING=14
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51
PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179
PRIMER_INTERNAL_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158
PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=6.738411
PRIMER_LEFT_0_PENALTY=4.596160
PRIMER_RIGHT_0_PENALTY=2.142251
PRIMER_INTERNAL_0_PENALTY=0.727969
PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_0_SEQUENCE=TGACCTGATAGAAACGCCAA
PRIMER_LEFT_0=30,24
PRIMER_RIGHT_0=129,18
PRIMER_INTERNAL_0=56,20
PRIMER_LEFT_0_TM=59.404
PRIMER_RIGHT_0_TM=60.142
PRIMER_INTERNAL_0_TM=59.272
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_0_PRODUCT_TM=69.9
=
SEQUENCE_ID=SP01-06-with-repeat2
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib
PRIMER_MAX_LIBRARY_MISPRIMING=14
PRIMER_INTERNAL_MISHYB_LIBRARY=../not_for_production.lib
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=14
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51
PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179
PRIMER_INTERNAL_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158
PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=6.738411
PRIMER_LEFT_0_PENALTY=4.596160
PRIMER_RIGHT_0_PENALTY=2.142251
PRIMER_INTERNAL_0_PENALTY=0.727969
PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_0_SEQUENCE=TGACCTGATAGAAACGCCAA
PRIMER_LEFT_0=30,24
PRIMER_RIGHT_0=129,18
PRIMER_INTERNAL_0=56,20
PRIMER_LEFT_0_TM=59.404
PRIMER_RIGHT_0_TM=60.142
PRIMER_INTERNAL_0_TM=59.272
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_INTERNAL_0_LIBRARY_MISHYB=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_0_PRODUCT_TM=69.9
=
SEQUENCE_ID=SP01-06-with-repeat3
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MISPRIMING_LIBRARY=
PRIMER_INTERNAL_MISHYB_LIBRARY=../not_for_production.lib
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=14
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51
PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179
PRIMER_INTERNAL_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158
PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=6.738411
PRIMER_LEFT_0_PENALTY=4.596160
PRIMER_RIGHT_0_PENALTY=2.142251
PRIMER_INTERNAL_0_PENALTY=0.727969
PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_0_SEQUENCE=TGACCTGATAGAAACGCCAA
PRIMER_LEFT_0=30,24
PRIMER_RIGHT_0=129,18
PRIMER_INTERNAL_0=56,20
PRIMER_LEFT_0_TM=59.404
PRIMER_RIGHT_0_TM=60.142
PRIMER_INTERNAL_0_TM=59.272
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_INTERNAL_0_LIBRARY_MISHYB=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_0_PRODUCT_TM=69.9
=
SEQUENCE_ID=case_problem_test
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_MISPRIMING_LIBRARY=
PRIMER_INTERNAL_MISHYB_LIBRARY=
SEQUENCE_TEMPLATE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat
SEQUENCE_PRIMER=acctgggcaccacctactc
PRIMER_PRODUCT_SIZE_RANGE=1890-1920
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 310, high end compl 194, ok 116
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1.103015
PRIMER_LEFT_0_PENALTY=1.022702
PRIMER_RIGHT_0_PENALTY=0.080313
PRIMER_LEFT_0_SEQUENCE=acctgggcaccacctactc
PRIMER_RIGHT_0_SEQUENCE=tctacctcctcaatggtggg
PRIMER_LEFT_0=28,19
PRIMER_RIGHT_0=1918,20
PRIMER_LEFT_0_TM=59.977
PRIMER_RIGHT_0_TM=59.920
PRIMER_LEFT_0_GC_PERCENT=63.158
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=1891
PRIMER_PAIR_0_PRODUCT_TM=85.5
=
SEQUENCE_ID=tm_out_of_range_on_input
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_MAX_TM=59.97
PRIMER_OPT_TM=59.97
SEQUENCE_TEMPLATE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat
SEQUENCE_PRIMER=acctgggcaccacctactc
PRIMER_PRODUCT_SIZE_RANGE=1890-1920
PRIMER_LEFT_EXPLAIN=considered 1, high tm 1, ok 0
PRIMER_RIGHT_EXPLAIN=considered 310, high tm 301, high end compl 3, ok 6
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
