PRIMER_PRODUCT_SIZE_RANGE=75-20000
PRIMER_MAX_END_STABILITY=10
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_SIZE=19
PRIMER_MAX_TM=75
PRIMER_NUM_RETURN=1
SEQUENCE_ID=test0
SEQUENCE_TEMPLATE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
SEQUENCE_START_CODON_POSITION=-3
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 14, ok 4
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=69.797698
PRIMER_LEFT_0_PENALTY=68.113745
PRIMER_RIGHT_0_PENALTY=1.683953
PRIMER_LEFT_0_SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAA
PRIMER_RIGHT_0_SEQUENCE=ACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=75,19
PRIMER_LEFT_0_TM=58.886
PRIMER_RIGHT_0_TM=60.684
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=60.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.7000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_0_PRODUCT_TM=70.9
=
SEQUENCE_ID=test1
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
SEQUENCE_START_CODON_POSITION=0
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=8.205547
PRIMER_LEFT_0_PENALTY=7.037233
PRIMER_RIGHT_0_PENALTY=1.168315
PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=TACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=76,20
PRIMER_LEFT_0_TM=57.963
PRIMER_RIGHT_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.500000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_0_PRODUCT_TM=71.3
=
SEQUENCE_ID=test1b with lower case codon
SEQUENCE_TEMPLATE=atgGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
SEQUENCE_START_CODON_POSITION=0
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=8.205547
PRIMER_LEFT_0_PENALTY=7.037233
PRIMER_RIGHT_0_PENALTY=1.168315
PRIMER_LEFT_0_SEQUENCE=atgGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=TACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=76,20
PRIMER_LEFT_0_TM=57.963
PRIMER_RIGHT_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.500000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_0_PRODUCT_TM=71.3
=
SEQUENCE_ID=test1c with special start codon
SEQUENCE_TEMPLATE=CGAGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
SEQUENCE_START_CODON_POSITION=0
SEQUENCE_START_CODON_SEQUENCE=CGA
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, ok 5
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.481051
PRIMER_LEFT_0_PENALTY=6.312737
PRIMER_RIGHT_0_PENALTY=1.168315
PRIMER_LEFT_0_SEQUENCE=CGAGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=TACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=76,20
PRIMER_LEFT_0_TM=58.687
PRIMER_RIGHT_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=44.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.500000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_0_PRODUCT_TM=71.8
=
SEQUENCE_ID=test1d with special start codon in lower case
SEQUENCE_TEMPLATE=CGAGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
SEQUENCE_START_CODON_POSITION=0
SEQUENCE_START_CODON_SEQUENCE=cga
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, ok 5
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.481051
PRIMER_LEFT_0_PENALTY=6.312737
PRIMER_RIGHT_0_PENALTY=1.168315
PRIMER_LEFT_0_SEQUENCE=CGAGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=TACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=76,20
PRIMER_LEFT_0_TM=58.687
PRIMER_RIGHT_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=44.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.500000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_0_PRODUCT_TM=71.8
=
SEQUENCE_ID=test1e with failing codon
SEQUENCE_TEMPLATE=CGAGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
SEQUENCE_START_CODON_POSITION=0
SEQUENCE_START_CODON_SEQUENCE=CGAG
PRIMER_ERROR=No codon provided in SEQUENCE_START_CODON_SEQUENCE
=
SEQUENCE_ID=test1f with failing codon
SEQUENCE_TEMPLATE=CGAGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
SEQUENCE_START_CODON_POSITION=0
SEQUENCE_START_CODON_SEQUENCE=CG
PRIMER_ERROR=No codon provided in SEQUENCE_START_CODON_SEQUENCE
=
SEQUENCE_ID=test2
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
SEQUENCE_START_CODON_POSITION=0
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=8.721185
PRIMER_LEFT_0_PENALTY=7.037233
PRIMER_RIGHT_0_PENALTY=1.683953
PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=ACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=75,19
PRIMER_LEFT_0_TM=57.963
PRIMER_RIGHT_0_TM=60.684
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_0_PRODUCT_TM=71.4
=
SEQUENCE_ID=test3
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
SEQUENCE_START_CODON_POSITION=0
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=75
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=8.721185
PRIMER_LEFT_0_PENALTY=7.037233
PRIMER_RIGHT_0_PENALTY=1.683953
PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=ACTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=75,19
PRIMER_LEFT_0_TM=57.963
PRIMER_RIGHT_0_TM=60.684
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_0_PRODUCT_TM=71.4
=
SEQUENCE_ID=test4
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
SEQUENCE_START_CODON_POSITION=0
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=-1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=9.930660
PRIMER_LEFT_0_PENALTY=7.037233
PRIMER_RIGHT_0_PENALTY=2.893427
PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
PRIMER_RIGHT_0_SEQUENCE=TATCTGAGCAAGGAAGCTCCG
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=77,21
PRIMER_LEFT_0_TM=57.963
PRIMER_RIGHT_0_TM=61.893
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_0_PRODUCT_TM=71.1
=
SEQUENCE_ID=test5
SEQUENCE_TEMPLATE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
SEQUENCE_START_CODON_POSITION=0
PRIMER_ERROR=No start codon at SEQUENCE_START_CODON_POSITION
=
SEQUENCE_ID=test6
SEQUENCE_INCLUDED_REGION=0,299
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA
SEQUENCE_START_CODON_POSITION=298
P3_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION".
PRIMER_ERROR=Start codon position not contained in SEQUENCE_INCLUDED_REGION
=
SEQUENCE_ID=test7
SEQUENCE_INCLUDED_REGION=0,300
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG
SEQUENCE_START_CODON_POSITION=297
P3_COMMENT=Should succeed.
PRIMER_LEFT_EXPLAIN=considered 1864, would not amplify any of the ORF 1229, low tm 87, high tm 53, high 3' stability 25, ok 470
PRIMER_RIGHT_EXPLAIN=considered 1840, low tm 186, high tm 161, high 3' stability 25, ok 1468
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_STOP_CODON_POSITION=-1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2163.830281
PRIMER_LEFT_0_PENALTY=2160.043439
PRIMER_RIGHT_0_PENALTY=3.786842
PRIMER_LEFT_0_SEQUENCE=AAAGATGGCATTCATCTGGC
PRIMER_RIGHT_0_SEQUENCE=CATTATCAGCAGAGACCCATTT
PRIMER_LEFT_0=225,20
PRIMER_RIGHT_0=299,22
PRIMER_LEFT_0_TM=60.043
PRIMER_RIGHT_0_TM=58.213
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_POSITION_PENALTY=2160.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_0_PRODUCT_TM=69.9
=
SEQUENCE_ID=test8
PRIMER_INSIDE_PENALTY=100.0
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
SEQUENCE_START_CODON_POSITION=0
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
PRIMER_ERROR=Cannot accept both SEQUENCE_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY
=
SEQUENCE_ID=test9
PRIMER_INSIDE_PENALTY=-1.0
PRIMER_OUTSIDE_PENALTY=100.0
SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
SEQUENCE_START_CODON_POSITION=0
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
PRIMER_ERROR=Cannot accept both SEQUENCE_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY
=
PRIMER_PRODUCT_SIZE_RANGE=75-20000
PRIMER_OUTSIDE_PENALTY=0.0
PRIMER_MAX_END_STABILITY=10
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_SIZE=19
PRIMER_MAX_TM=65
PRIMER_NUM_RETURN=20
SEQUENCE_ID=DCC/X76132
[encoded data, 4626 characters]
SEQUENCE_START_CODON_POSITION=0
PRIMER_LEFT_EXPLAIN=considered 39774, would not amplify any of the ORF 27031, GC content failed 49, low tm 2568, high tm 5293, long poly-x seq 73, high 3' stability 94, ok 4666
PRIMER_RIGHT_EXPLAIN=considered 39257, GC content failed 148, low tm 8235, high tm 15926, high any compl 53, high end compl 11, long poly-x seq 76, high 3' stability 140, ok 14668
PRIMER_PAIR_EXPLAIN=considered 22, ok 22
PRIMER_STOP_CODON_POSITION=4341
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=20.422177
PRIMER_LEFT_0_PENALTY=7.184184
PRIMER_RIGHT_0_PENALTY=13.237993
PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_0_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=4356,25
PRIMER_LEFT_0_TM=60.184
PRIMER_RIGHT_0_TM=59.262
PRIMER_LEFT_0_GC_PERCENT=37.037
PRIMER_RIGHT_0_GC_PERCENT=32.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=7.500000
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=4357
PRIMER_PAIR_0_PRODUCT_TM=79.8
PRIMER_PAIR_1_PENALTY=20.559156
PRIMER_LEFT_1_PENALTY=7.184184
PRIMER_RIGHT_1_PENALTY=13.374972
PRIMER_LEFT_1_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_1_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_1=0,27
PRIMER_RIGHT_1=4355,24
PRIMER_LEFT_1_TM=60.184
PRIMER_RIGHT_1_TM=57.625
PRIMER_LEFT_1_GC_PERCENT=37.037
PRIMER_RIGHT_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=7.000000
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=4356
PRIMER_PAIR_1_PRODUCT_TM=79.8
PRIMER_PAIR_2_PENALTY=20.657955
PRIMER_LEFT_2_PENALTY=7.184184
PRIMER_RIGHT_2_PENALTY=13.473771
PRIMER_LEFT_2_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_2_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG
PRIMER_LEFT_2=0,27
PRIMER_RIGHT_2=4355,25
PRIMER_LEFT_2_TM=60.184
PRIMER_RIGHT_2_TM=58.526
PRIMER_LEFT_2_GC_PERCENT=37.037
PRIMER_RIGHT_2_GC_PERCENT=36.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=7.000000
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=4356
PRIMER_PAIR_2_PRODUCT_TM=79.8
PRIMER_PAIR_3_PENALTY=20.761130
PRIMER_LEFT_3_PENALTY=7.184184
PRIMER_RIGHT_3_PENALTY=13.576946
PRIMER_LEFT_3_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_3_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG
PRIMER_LEFT_3=0,27
PRIMER_RIGHT_3=4356,26
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=60.077
PRIMER_LEFT_3_GC_PERCENT=37.037
PRIMER_RIGHT_3_GC_PERCENT=34.615
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=7.500000
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=4357
PRIMER_PAIR_3_PRODUCT_TM=79.8
PRIMER_PAIR_4_PENALTY=21.059156
PRIMER_LEFT_4_PENALTY=7.184184
PRIMER_RIGHT_4_PENALTY=13.874972
PRIMER_LEFT_4_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_4_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG
PRIMER_LEFT_4=0,27
PRIMER_RIGHT_4=4356,24
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=57.625
PRIMER_LEFT_4_GC_PERCENT=37.037
PRIMER_RIGHT_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=7.500000
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=4357
PRIMER_PAIR_4_PRODUCT_TM=79.8
PRIMER_PAIR_5_PENALTY=21.335332
PRIMER_LEFT_5_PENALTY=7.184184
PRIMER_RIGHT_5_PENALTY=14.151147
PRIMER_LEFT_5_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_5_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG
PRIMER_LEFT_5=0,27
PRIMER_RIGHT_5=4357,25
PRIMER_LEFT_5_TM=60.184
PRIMER_RIGHT_5_TM=58.849
PRIMER_LEFT_5_GC_PERCENT=37.037
PRIMER_RIGHT_5_GC_PERCENT=32.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=8.000000
PRIMER_LEFT_5_END_STABILITY=8.8000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=4358
PRIMER_PAIR_5_PRODUCT_TM=79.8
PRIMER_PAIR_6_PENALTY=21.566466
PRIMER_LEFT_6_PENALTY=7.184184
PRIMER_RIGHT_6_PENALTY=14.382282
PRIMER_LEFT_6_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_6_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_6=0,27
PRIMER_RIGHT_6=4357,26
PRIMER_LEFT_6_TM=60.184
PRIMER_RIGHT_6_TM=60.382
PRIMER_LEFT_6_GC_PERCENT=37.037
PRIMER_RIGHT_6_GC_PERCENT=30.769
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=8.000000
PRIMER_LEFT_6_END_STABILITY=8.8000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=4358
PRIMER_PAIR_6_PRODUCT_TM=79.8
PRIMER_PAIR_7_PENALTY=22.076208
PRIMER_LEFT_7_PENALTY=8.838215
PRIMER_RIGHT_7_PENALTY=13.237993
PRIMER_LEFT_7_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_7_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_7=0,26
PRIMER_RIGHT_7=4356,25
PRIMER_LEFT_7_TM=57.162
PRIMER_RIGHT_7_TM=59.262
PRIMER_LEFT_7_GC_PERCENT=34.615
PRIMER_RIGHT_7_GC_PERCENT=32.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=7.500000
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=4357
PRIMER_PAIR_7_PRODUCT_TM=79.8
PRIMER_PAIR_8_PENALTY=22.213187
PRIMER_LEFT_8_PENALTY=8.838215
PRIMER_RIGHT_8_PENALTY=13.374972
PRIMER_LEFT_8_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_8_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_8=0,26
PRIMER_RIGHT_8=4355,24
PRIMER_LEFT_8_TM=57.162
PRIMER_RIGHT_8_TM=57.625
PRIMER_LEFT_8_GC_PERCENT=34.615
PRIMER_RIGHT_8_GC_PERCENT=33.333
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=7.000000
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=4356
PRIMER_PAIR_8_PRODUCT_TM=79.8
PRIMER_PAIR_9_PENALTY=22.311986
PRIMER_LEFT_9_PENALTY=8.838215
PRIMER_RIGHT_9_PENALTY=13.473771
PRIMER_LEFT_9_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_9_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG
PRIMER_LEFT_9=0,26
PRIMER_RIGHT_9=4355,25
PRIMER_LEFT_9_TM=57.162
PRIMER_RIGHT_9_TM=58.526
PRIMER_LEFT_9_GC_PERCENT=34.615
PRIMER_RIGHT_9_GC_PERCENT=36.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=7.000000
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=4356
PRIMER_PAIR_9_PRODUCT_TM=79.8
PRIMER_PAIR_10_PENALTY=22.415161
PRIMER_LEFT_10_PENALTY=8.838215
PRIMER_RIGHT_10_PENALTY=13.576946
PRIMER_LEFT_10_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_10_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG
PRIMER_LEFT_10=0,26
PRIMER_RIGHT_10=4356,26
PRIMER_LEFT_10_TM=57.162
PRIMER_RIGHT_10_TM=60.077
PRIMER_LEFT_10_GC_PERCENT=34.615
PRIMER_RIGHT_10_GC_PERCENT=34.615
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=7.500000
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=4357
PRIMER_PAIR_10_PRODUCT_TM=79.8
PRIMER_PAIR_11_PENALTY=22.564003
PRIMER_LEFT_11_PENALTY=7.184184
PRIMER_RIGHT_11_PENALTY=15.379819
PRIMER_LEFT_11_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_11_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTG
PRIMER_LEFT_11=0,27
PRIMER_RIGHT_11=4358,26
PRIMER_LEFT_11_TM=60.184
PRIMER_RIGHT_11_TM=59.120
PRIMER_LEFT_11_GC_PERCENT=37.037
PRIMER_RIGHT_11_GC_PERCENT=30.769
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=8.500000
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=4359
PRIMER_PAIR_11_PRODUCT_TM=79.8
PRIMER_PAIR_12_PENALTY=22.713187
PRIMER_LEFT_12_PENALTY=8.838215
PRIMER_RIGHT_12_PENALTY=13.874972
PRIMER_LEFT_12_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_12_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG
PRIMER_LEFT_12=0,26
PRIMER_RIGHT_12=4356,24
PRIMER_LEFT_12_TM=57.162
PRIMER_RIGHT_12_TM=57.625
PRIMER_LEFT_12_GC_PERCENT=34.615
PRIMER_RIGHT_12_GC_PERCENT=33.333
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=7.500000
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=4357
PRIMER_PAIR_12_PRODUCT_TM=79.8
PRIMER_PAIR_13_PENALTY=22.895624
PRIMER_LEFT_13_PENALTY=7.184184
PRIMER_RIGHT_13_PENALTY=15.711440
PRIMER_LEFT_13_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_13_SEQUENCE=CATTCAGAAATACATGTTAAAAGGC
PRIMER_LEFT_13=0,27
PRIMER_RIGHT_13=4359,25
PRIMER_LEFT_13_TM=60.184
PRIMER_RIGHT_13_TM=58.289
PRIMER_LEFT_13_GC_PERCENT=37.037
PRIMER_RIGHT_13_GC_PERCENT=32.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=9.000000
PRIMER_LEFT_13_END_STABILITY=8.8000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=4360
PRIMER_PAIR_13_PRODUCT_TM=79.8
PRIMER_PAIR_14_PENALTY=22.989362
PRIMER_LEFT_14_PENALTY=8.838215
PRIMER_RIGHT_14_PENALTY=14.151147
PRIMER_LEFT_14_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_14_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG
PRIMER_LEFT_14=0,26
PRIMER_RIGHT_14=4357,25
PRIMER_LEFT_14_TM=57.162
PRIMER_RIGHT_14_TM=58.849
PRIMER_LEFT_14_GC_PERCENT=34.615
PRIMER_RIGHT_14_GC_PERCENT=32.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=8.000000
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=4358
PRIMER_PAIR_14_PRODUCT_TM=79.8
PRIMER_PAIR_15_PENALTY=23.064003
PRIMER_LEFT_15_PENALTY=7.184184
PRIMER_RIGHT_15_PENALTY=15.879819
PRIMER_LEFT_15_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_15_SEQUENCE=CATTCAGAAATACATGTTAAAAGGCT
PRIMER_LEFT_15=0,27
PRIMER_RIGHT_15=4359,26
PRIMER_LEFT_15_TM=60.184
PRIMER_RIGHT_15_TM=59.120
PRIMER_LEFT_15_GC_PERCENT=37.037
PRIMER_RIGHT_15_GC_PERCENT=30.769
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=9.000000
PRIMER_LEFT_15_END_STABILITY=8.8000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=4360
PRIMER_PAIR_15_PRODUCT_TM=79.8
PRIMER_PAIR_16_PENALTY=23.220496
PRIMER_LEFT_16_PENALTY=8.838215
PRIMER_RIGHT_16_PENALTY=14.382282
PRIMER_LEFT_16_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
PRIMER_RIGHT_16_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_16=0,26
PRIMER_RIGHT_16=4357,26
PRIMER_LEFT_16_TM=57.162
PRIMER_RIGHT_16_TM=60.382
PRIMER_LEFT_16_GC_PERCENT=34.615
PRIMER_RIGHT_16_GC_PERCENT=30.769
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=8.000000
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=4358
PRIMER_PAIR_16_PRODUCT_TM=79.8
PRIMER_PAIR_17_PENALTY=23.266175
PRIMER_LEFT_17_PENALTY=7.184184
PRIMER_RIGHT_17_PENALTY=16.081991
PRIMER_LEFT_17_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_17_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTGA
PRIMER_LEFT_17=0,27
PRIMER_RIGHT_17=4358,27
PRIMER_LEFT_17_TM=60.184
PRIMER_RIGHT_17_TM=60.582
PRIMER_LEFT_17_GC_PERCENT=37.037
PRIMER_RIGHT_17_GC_PERCENT=29.630
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=8.500000
PRIMER_LEFT_17_END_STABILITY=8.8000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=4359
PRIMER_PAIR_17_PRODUCT_TM=79.8
PRIMER_PAIR_18_PENALTY=23.304836
PRIMER_LEFT_18_PENALTY=7.184184
PRIMER_RIGHT_18_PENALTY=16.120652
PRIMER_LEFT_18_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_18_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGAG
PRIMER_LEFT_18=0,27
PRIMER_RIGHT_18=4357,27
PRIMER_LEFT_18_TM=60.184
PRIMER_RIGHT_18_TM=61.121
PRIMER_LEFT_18_GC_PERCENT=37.037
PRIMER_RIGHT_18_GC_PERCENT=33.333
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=8.000000
PRIMER_LEFT_18_END_STABILITY=8.8000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=4358
PRIMER_PAIR_18_PRODUCT_TM=79.8
PRIMER_PAIR_19_PENALTY=23.871468
PRIMER_LEFT_19_PENALTY=7.184184
PRIMER_RIGHT_19_PENALTY=16.687284
PRIMER_LEFT_19_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
PRIMER_RIGHT_19_SEQUENCE=CCATTCAGAAATACATGTTAAAAGGC
PRIMER_LEFT_19=0,27
PRIMER_RIGHT_19=4360,26
PRIMER_LEFT_19_TM=60.184
PRIMER_RIGHT_19_TM=61.187
PRIMER_LEFT_19_GC_PERCENT=37.037
PRIMER_RIGHT_19_GC_PERCENT=34.615
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=9.500000
PRIMER_LEFT_19_END_STABILITY=8.8000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=4361
PRIMER_PAIR_19_PRODUCT_TM=79.8
=
SEQUENCE_ID=LEU8/L-SELECTING/X17519
SEQUENCE_TEMPLATE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT
SEQUENCE_START_CODON_POSITION=49
PRIMER_LEFT_EXPLAIN=considered 19864, would not amplify any of the ORF 16423, low tm 794, high tm 1127, high end compl 1, long poly-x seq 23, high 3' stability 37, ok 1459
PRIMER_RIGHT_EXPLAIN=considered 19472, GC content failed 127, low tm 6190, high tm 5041, high end compl 1, long poly-x seq 45, high 3' stability 67, ok 8001
PRIMER_PAIR_EXPLAIN=considered 24, high end compl 2, ok 22
PRIMER_STOP_CODON_POSITION=1204
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=6.788400
PRIMER_LEFT_0_PENALTY=1.909478
PRIMER_RIGHT_0_PENALTY=4.878922
PRIMER_LEFT_0_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_0_SEQUENCE=GGGCGATTTAATATGGGTCA
PRIMER_LEFT_0=49,21
PRIMER_RIGHT_0=1213,20
PRIMER_LEFT_0_TM=59.091
PRIMER_RIGHT_0_TM=59.621
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=4.500000
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=1165
PRIMER_PAIR_0_PRODUCT_TM=78.2
PRIMER_PAIR_1_PENALTY=6.918802
PRIMER_LEFT_1_PENALTY=2.039879
PRIMER_RIGHT_1_PENALTY=4.878922
PRIMER_LEFT_1_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_1_SEQUENCE=GGGCGATTTAATATGGGTCA
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=1213,20
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.621
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=4.500000
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=1165
PRIMER_PAIR_1_PRODUCT_TM=78.2
PRIMER_PAIR_2_PENALTY=7.515951
PRIMER_LEFT_2_PENALTY=1.909478
PRIMER_RIGHT_2_PENALTY=5.606474
PRIMER_LEFT_2_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_2_SEQUENCE=GGGCGATTTAATATGGGTCAT
PRIMER_LEFT_2=49,21
PRIMER_RIGHT_2=1213,21
PRIMER_LEFT_2_TM=59.091
PRIMER_RIGHT_2_TM=59.894
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=4.500000
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=1165
PRIMER_PAIR_2_PRODUCT_TM=78.2
PRIMER_PAIR_3_PENALTY=7.646353
PRIMER_LEFT_3_PENALTY=2.039879
PRIMER_RIGHT_3_PENALTY=5.606474
PRIMER_LEFT_3_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_3_SEQUENCE=GGGCGATTTAATATGGGTCAT
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=1213,21
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.894
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=4.500000
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=1165
PRIMER_PAIR_3_PRODUCT_TM=78.2
PRIMER_PAIR_4_PENALTY=8.353697
PRIMER_LEFT_4_PENALTY=1.909478
PRIMER_RIGHT_4_PENALTY=6.444219
PRIMER_LEFT_4_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_4_SEQUENCE=GGCGATTTAATATGGGTCATTC
PRIMER_LEFT_4=49,21
PRIMER_RIGHT_4=1212,22
PRIMER_LEFT_4_TM=59.091
PRIMER_RIGHT_4_TM=59.556
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=4.000000
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=1164
PRIMER_PAIR_4_PRODUCT_TM=78.2
PRIMER_PAIR_5_PENALTY=8.431625
PRIMER_LEFT_5_PENALTY=1.909478
PRIMER_RIGHT_5_PENALTY=6.522147
PRIMER_LEFT_5_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_5_SEQUENCE=AGGGCGATTTAATATGGGTCA
PRIMER_LEFT_5=49,21
PRIMER_RIGHT_5=1214,21
PRIMER_LEFT_5_TM=59.091
PRIMER_RIGHT_5_TM=60.522
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=1166
PRIMER_PAIR_5_PRODUCT_TM=78.2
PRIMER_PAIR_6_PENALTY=8.484099
PRIMER_LEFT_6_PENALTY=2.039879
PRIMER_RIGHT_6_PENALTY=6.444219
PRIMER_LEFT_6_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_6_SEQUENCE=GGCGATTTAATATGGGTCATTC
PRIMER_LEFT_6=49,22
PRIMER_RIGHT_6=1212,22
PRIMER_LEFT_6_TM=60.040
PRIMER_RIGHT_6_TM=59.556
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=4.000000
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=1164
PRIMER_PAIR_6_PRODUCT_TM=78.2
PRIMER_PAIR_7_PENALTY=8.562026
PRIMER_LEFT_7_PENALTY=2.039879
PRIMER_RIGHT_7_PENALTY=6.522147
PRIMER_LEFT_7_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_7_SEQUENCE=AGGGCGATTTAATATGGGTCA
PRIMER_LEFT_7=49,22
PRIMER_RIGHT_7=1214,21
PRIMER_LEFT_7_TM=60.040
PRIMER_RIGHT_7_TM=60.522
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=1166
PRIMER_PAIR_7_PRODUCT_TM=78.2
PRIMER_PAIR_8_PENALTY=8.579842
PRIMER_LEFT_8_PENALTY=1.909478
PRIMER_RIGHT_8_PENALTY=6.670364
PRIMER_LEFT_8_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_8_SEQUENCE=GCGATTTAATATGGGTCATTCA
PRIMER_LEFT_8=49,21
PRIMER_RIGHT_8=1211,22
PRIMER_LEFT_8_TM=59.091
PRIMER_RIGHT_8_TM=58.830
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=36.364
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
PRIMER_LEFT_8_END_STABILITY=5.7000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=1163
PRIMER_PAIR_8_PRODUCT_TM=78.1
PRIMER_PAIR_9_PENALTY=8.631677
PRIMER_LEFT_9_PENALTY=1.909478
PRIMER_RIGHT_9_PENALTY=6.722200
PRIMER_LEFT_9_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_9_SEQUENCE=CAAGGGCGATTTAATATGGG
PRIMER_LEFT_9=49,21
PRIMER_RIGHT_9=1216,20
PRIMER_LEFT_9_TM=59.091
PRIMER_RIGHT_9_TM=59.278
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=6.000000
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=1168
PRIMER_PAIR_9_PRODUCT_TM=78.2
PRIMER_PAIR_10_PENALTY=8.710244
PRIMER_LEFT_10_PENALTY=2.039879
PRIMER_RIGHT_10_PENALTY=6.670364
PRIMER_LEFT_10_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_10_SEQUENCE=GCGATTTAATATGGGTCATTCA
PRIMER_LEFT_10=49,22
PRIMER_RIGHT_10=1211,22
PRIMER_LEFT_10_TM=60.040
PRIMER_RIGHT_10_TM=58.830
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=36.364
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=3.500000
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=1163
PRIMER_PAIR_10_PRODUCT_TM=78.1
PRIMER_PAIR_11_PENALTY=8.762079
PRIMER_LEFT_11_PENALTY=2.039879
PRIMER_RIGHT_11_PENALTY=6.722200
PRIMER_LEFT_11_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_11_SEQUENCE=CAAGGGCGATTTAATATGGG
PRIMER_LEFT_11=49,22
PRIMER_RIGHT_11=1216,20
PRIMER_LEFT_11_TM=60.040
PRIMER_RIGHT_11_TM=59.278
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=6.000000
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=1168
PRIMER_PAIR_11_PRODUCT_TM=78.2
PRIMER_PAIR_12_PENALTY=8.958439
PRIMER_LEFT_12_PENALTY=1.909478
PRIMER_RIGHT_12_PENALTY=7.048962
PRIMER_LEFT_12_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_12_SEQUENCE=CAAGGGCGATTTAATATGGGT
PRIMER_LEFT_12=49,21
PRIMER_RIGHT_12=1216,21
PRIMER_LEFT_12_TM=59.091
PRIMER_RIGHT_12_TM=60.049
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=6.000000
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=2.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=1168
PRIMER_PAIR_12_PRODUCT_TM=78.2
PRIMER_PAIR_13_PENALTY=9.013864
PRIMER_LEFT_13_PENALTY=1.909478
PRIMER_RIGHT_13_PENALTY=7.104387
PRIMER_LEFT_13_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_13_SEQUENCE=AGGGCGATTTAATATGGGTC
PRIMER_LEFT_13=49,21
PRIMER_RIGHT_13=1214,20
PRIMER_LEFT_13_TM=59.091
PRIMER_RIGHT_13_TM=57.896
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=5.000000
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=9.1000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=1166
PRIMER_PAIR_13_PRODUCT_TM=78.2
PRIMER_PAIR_14_PENALTY=9.067167
PRIMER_LEFT_14_PENALTY=1.909478
PRIMER_RIGHT_14_PENALTY=7.157689
PRIMER_LEFT_14_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_14_SEQUENCE=GGCGATTTAATATGGGTCATT
PRIMER_LEFT_14=49,21
PRIMER_RIGHT_14=1212,21
PRIMER_LEFT_14_TM=59.091
PRIMER_RIGHT_14_TM=57.842
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=38.095
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=4.000000
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=1164
PRIMER_PAIR_14_PRODUCT_TM=78.2
PRIMER_PAIR_15_PENALTY=9.088841
PRIMER_LEFT_15_PENALTY=2.039879
PRIMER_RIGHT_15_PENALTY=7.048962
PRIMER_LEFT_15_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_15_SEQUENCE=CAAGGGCGATTTAATATGGGT
PRIMER_LEFT_15=49,22
PRIMER_RIGHT_15=1216,21
PRIMER_LEFT_15_TM=60.040
PRIMER_RIGHT_15_TM=60.049
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=6.000000
PRIMER_LEFT_15_END_STABILITY=5.7000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=1168
PRIMER_PAIR_15_PRODUCT_TM=78.2
PRIMER_PAIR_16_PENALTY=9.144266
PRIMER_LEFT_16_PENALTY=2.039879
PRIMER_RIGHT_16_PENALTY=7.104387
PRIMER_LEFT_16_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_16_SEQUENCE=AGGGCGATTTAATATGGGTC
PRIMER_LEFT_16=49,22
PRIMER_RIGHT_16=1214,20
PRIMER_LEFT_16_TM=60.040
PRIMER_RIGHT_16_TM=57.896
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=5.000000
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=9.1000
PRIMER_PAIR_16_COMPL_ANY=2.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=1166
PRIMER_PAIR_16_PRODUCT_TM=78.2
PRIMER_PAIR_17_PENALTY=9.197568
PRIMER_LEFT_17_PENALTY=2.039879
PRIMER_RIGHT_17_PENALTY=7.157689
PRIMER_LEFT_17_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
PRIMER_RIGHT_17_SEQUENCE=GGCGATTTAATATGGGTCATT
PRIMER_LEFT_17=49,22
PRIMER_RIGHT_17=1212,21
PRIMER_LEFT_17_TM=60.040
PRIMER_RIGHT_17_TM=57.842
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=38.095
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=4.000000
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=1164
PRIMER_PAIR_17_PRODUCT_TM=78.2
PRIMER_PAIR_18_PENALTY=9.207691
PRIMER_LEFT_18_PENALTY=1.909478
PRIMER_RIGHT_18_PENALTY=7.298213
PRIMER_LEFT_18_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_18_SEQUENCE=AAGGGCGATTTAATATGGGTC
PRIMER_LEFT_18=49,21
PRIMER_RIGHT_18=1215,21
PRIMER_LEFT_18_TM=59.091
PRIMER_RIGHT_18_TM=59.202
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=5.500000
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=9.1000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=1167
PRIMER_PAIR_18_PRODUCT_TM=78.1
PRIMER_PAIR_19_PENALTY=9.281084
PRIMER_LEFT_19_PENALTY=1.909478
PRIMER_RIGHT_19_PENALTY=7.371606
PRIMER_LEFT_19_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
PRIMER_RIGHT_19_SEQUENCE=GCGATTTAATATGGGTCATTCAT
PRIMER_LEFT_19=49,21
PRIMER_RIGHT_19=1211,23
PRIMER_LEFT_19_TM=59.091
PRIMER_RIGHT_19_TM=59.128
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=34.783
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=3.500000
PRIMER_LEFT_19_END_STABILITY=5.7000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=1163
PRIMER_PAIR_19_PRODUCT_TM=78.1
=
SEQUENCE_ID=L40146/UBC4
SEQUENCE_START_CODON_POSITION=0
SEQUENCE_TEMPLATE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA
PRIMER_LEFT_EXPLAIN=considered 3285, would not amplify any of the ORF 2182, GC content failed 11, low tm 330, high tm 255, high end compl 1, high 3' stability 7, ok 499
PRIMER_RIGHT_EXPLAIN=considered 3267, GC content failed 36, low tm 1201, high tm 523, high 3' stability 8, ok 1499
PRIMER_PAIR_EXPLAIN=considered 56, high end compl 34, ok 22
PRIMER_STOP_CODON_POSITION=441
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=6.820809
PRIMER_LEFT_0_PENALTY=4.584153
PRIMER_RIGHT_0_PENALTY=2.236656
PRIMER_LEFT_0_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
PRIMER_RIGHT_0_SEQUENCE=ACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_0=0,21
PRIMER_RIGHT_0=441,22
PRIMER_LEFT_0_TM=63.584
PRIMER_RIGHT_0_TM=59.763
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=442
PRIMER_PAIR_0_PRODUCT_TM=75.9
PRIMER_PAIR_1_PENALTY=8.301465
PRIMER_LEFT_1_PENALTY=4.584153
PRIMER_RIGHT_1_PENALTY=3.717312
PRIMER_LEFT_1_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
PRIMER_RIGHT_1_SEQUENCE=TACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=442,23
PRIMER_LEFT_1_TM=63.584
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.500000
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=443
PRIMER_PAIR_1_PRODUCT_TM=75.9
PRIMER_PAIR_2_PENALTY=8.494006
PRIMER_LEFT_2_PENALTY=2.909495
PRIMER_RIGHT_2_PENALTY=5.584511
PRIMER_LEFT_2_SEQUENCE=ATGGCGCTGAAAAGAATCCA
PRIMER_RIGHT_2_SEQUENCE=TTACATCGCATACTTCTGAGTCC
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=443,23
PRIMER_LEFT_2_TM=62.909
PRIMER_RIGHT_2_TM=58.415
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=444
PRIMER_PAIR_2_PRODUCT_TM=75.8
PRIMER_PAIR_3_PENALTY=10.168664
PRIMER_LEFT_3_PENALTY=4.584153
PRIMER_RIGHT_3_PENALTY=5.584511
PRIMER_LEFT_3_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
PRIMER_RIGHT_3_SEQUENCE=TTACATCGCATACTTCTGAGTCC
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=443,23
PRIMER_LEFT_3_TM=63.584
PRIMER_RIGHT_3_TM=58.415
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=444
PRIMER_PAIR_3_PRODUCT_TM=75.8
PRIMER_PAIR_4_PENALTY=10.604982
PRIMER_LEFT_4_PENALTY=4.584153
PRIMER_RIGHT_4_PENALTY=6.020829
PRIMER_LEFT_4_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
PRIMER_RIGHT_4_SEQUENCE=TTACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_4=0,21
PRIMER_RIGHT_4=443,24
PRIMER_LEFT_4_TM=63.584
PRIMER_RIGHT_4_TM=61.021
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=1.000000
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=444
PRIMER_PAIR_4_PRODUCT_TM=75.8
PRIMER_PAIR_5_PENALTY=63.296939
PRIMER_LEFT_5_PENALTY=61.060283
PRIMER_RIGHT_5_PENALTY=2.236656
PRIMER_LEFT_5_SEQUENCE=GCGCTGAAAAGAATCCACA
PRIMER_RIGHT_5_SEQUENCE=ACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_5=3,19
PRIMER_RIGHT_5=441,22
PRIMER_LEFT_5_TM=59.940
PRIMER_RIGHT_5_TM=59.763
PRIMER_LEFT_5_GC_PERCENT=47.368
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_POSITION_PENALTY=60.000000
PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=439
PRIMER_PAIR_5_PRODUCT_TM=75.9
PRIMER_PAIR_6_PENALTY=63.521548
PRIMER_LEFT_6_PENALTY=61.284892
PRIMER_RIGHT_6_PENALTY=2.236656
PRIMER_LEFT_6_SEQUENCE=GCGCTGAAAAGAATCCACAA
PRIMER_RIGHT_6_SEQUENCE=ACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_6=3,20
PRIMER_RIGHT_6=441,22
PRIMER_LEFT_6_TM=61.285
PRIMER_RIGHT_6_TM=59.763
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_POSITION_PENALTY=60.000000
PRIMER_RIGHT_6_POSITION_PENALTY=0.000000
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=439
PRIMER_PAIR_6_PRODUCT_TM=75.9
PRIMER_PAIR_7_PENALTY=64.099814
PRIMER_LEFT_7_PENALTY=61.060283
PRIMER_RIGHT_7_PENALTY=3.039531
PRIMER_LEFT_7_SEQUENCE=GCGCTGAAAAGAATCCACA
PRIMER_RIGHT_7_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
PRIMER_LEFT_7=3,19
PRIMER_RIGHT_7=441,23
PRIMER_LEFT_7_TM=59.940
PRIMER_RIGHT_7_TM=60.040
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_POSITION_PENALTY=60.000000
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=439
PRIMER_PAIR_7_PRODUCT_TM=75.9
PRIMER_PAIR_8_PENALTY=64.324423
PRIMER_LEFT_8_PENALTY=61.284892
PRIMER_RIGHT_8_PENALTY=3.039531
PRIMER_LEFT_8_SEQUENCE=GCGCTGAAAAGAATCCACAA
PRIMER_RIGHT_8_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
PRIMER_LEFT_8=3,20
PRIMER_RIGHT_8=441,23
PRIMER_LEFT_8_TM=61.285
PRIMER_RIGHT_8_TM=60.040
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_POSITION_PENALTY=60.000000
PRIMER_RIGHT_8_POSITION_PENALTY=0.000000
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=439
PRIMER_PAIR_8_PRODUCT_TM=75.9
PRIMER_PAIR_9_PENALTY=64.777595
PRIMER_LEFT_9_PENALTY=61.060283
PRIMER_RIGHT_9_PENALTY=3.717312
PRIMER_LEFT_9_SEQUENCE=GCGCTGAAAAGAATCCACA
PRIMER_RIGHT_9_SEQUENCE=TACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_9=3,19
PRIMER_RIGHT_9=442,23
PRIMER_LEFT_9_TM=59.940
PRIMER_RIGHT_9_TM=59.783
PRIMER_LEFT_9_GC_PERCENT=47.368
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_POSITION_PENALTY=60.000000
PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=440
PRIMER_PAIR_9_PRODUCT_TM=75.9
PRIMER_PAIR_10_PENALTY=65.002204
PRIMER_LEFT_10_PENALTY=61.284892
PRIMER_RIGHT_10_PENALTY=3.717312
PRIMER_LEFT_10_SEQUENCE=GCGCTGAAAAGAATCCACAA
PRIMER_RIGHT_10_SEQUENCE=TACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_10=3,20
PRIMER_RIGHT_10=442,23
PRIMER_LEFT_10_TM=61.285
PRIMER_RIGHT_10_TM=59.783
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_POSITION_PENALTY=60.000000
PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=440
PRIMER_PAIR_10_PRODUCT_TM=75.9
PRIMER_PAIR_11_PENALTY=65.383808
PRIMER_LEFT_11_PENALTY=63.147152
PRIMER_RIGHT_11_PENALTY=2.236656
PRIMER_LEFT_11_SEQUENCE=GCGCTGAAAAGAATCCACAAG
PRIMER_RIGHT_11_SEQUENCE=ACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_11=3,21
PRIMER_RIGHT_11=441,22
PRIMER_LEFT_11_TM=62.147
PRIMER_RIGHT_11_TM=59.763
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_POSITION_PENALTY=60.000000
PRIMER_RIGHT_11_POSITION_PENALTY=0.000000
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=439
PRIMER_PAIR_11_PRODUCT_TM=75.9
PRIMER_PAIR_12_PENALTY=65.608414
PRIMER_LEFT_12_PENALTY=61.060283
PRIMER_RIGHT_12_PENALTY=4.548131
PRIMER_LEFT_12_SEQUENCE=GCGCTGAAAAGAATCCACA
PRIMER_RIGHT_12_SEQUENCE=TACATCGCATACTTCTGAGTCCAT
PRIMER_LEFT_12=3,19
PRIMER_RIGHT_12=442,24
PRIMER_LEFT_12_TM=59.940
PRIMER_RIGHT_12_TM=60.048
PRIMER_LEFT_12_GC_PERCENT=47.368
PRIMER_RIGHT_12_GC_PERCENT=41.667
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=60.000000
PRIMER_RIGHT_12_POSITION_PENALTY=0.500000
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=440
PRIMER_PAIR_12_PRODUCT_TM=75.9
PRIMER_PAIR_13_PENALTY=65.833022
PRIMER_LEFT_13_PENALTY=61.284892
PRIMER_RIGHT_13_PENALTY=4.548131
PRIMER_LEFT_13_SEQUENCE=GCGCTGAAAAGAATCCACAA
PRIMER_RIGHT_13_SEQUENCE=TACATCGCATACTTCTGAGTCCAT
PRIMER_LEFT_13=3,20
PRIMER_RIGHT_13=442,24
PRIMER_LEFT_13_TM=61.285
PRIMER_RIGHT_13_TM=60.048
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=41.667
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_POSITION_PENALTY=60.000000
PRIMER_RIGHT_13_POSITION_PENALTY=0.500000
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=440
PRIMER_PAIR_13_PRODUCT_TM=75.9
PRIMER_PAIR_14_PENALTY=66.186683
PRIMER_LEFT_14_PENALTY=63.147152
PRIMER_RIGHT_14_PENALTY=3.039531
PRIMER_LEFT_14_SEQUENCE=GCGCTGAAAAGAATCCACAAG
PRIMER_RIGHT_14_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
PRIMER_LEFT_14=3,21
PRIMER_RIGHT_14=441,23
PRIMER_LEFT_14_TM=62.147
PRIMER_RIGHT_14_TM=60.040
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_POSITION_PENALTY=60.000000
PRIMER_RIGHT_14_POSITION_PENALTY=0.000000
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=439
PRIMER_PAIR_14_PRODUCT_TM=75.9
PRIMER_PAIR_15_PENALTY=66.307348
PRIMER_LEFT_15_PENALTY=61.060283
PRIMER_RIGHT_15_PENALTY=5.247065
PRIMER_LEFT_15_SEQUENCE=GCGCTGAAAAGAATCCACA
PRIMER_RIGHT_15_SEQUENCE=ACATCGCATACTTCTGAGTCCATT
PRIMER_LEFT_15=3,19
PRIMER_RIGHT_15=441,24
PRIMER_LEFT_15_TM=59.940
PRIMER_RIGHT_15_TM=61.247
PRIMER_LEFT_15_GC_PERCENT=47.368
PRIMER_RIGHT_15_GC_PERCENT=41.667
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_POSITION_PENALTY=60.000000
PRIMER_RIGHT_15_POSITION_PENALTY=0.000000
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=439
PRIMER_PAIR_15_PRODUCT_TM=75.9
PRIMER_PAIR_16_PENALTY=66.531957
PRIMER_LEFT_16_PENALTY=61.284892
PRIMER_RIGHT_16_PENALTY=5.247065
PRIMER_LEFT_16_SEQUENCE=GCGCTGAAAAGAATCCACAA
PRIMER_RIGHT_16_SEQUENCE=ACATCGCATACTTCTGAGTCCATT
PRIMER_LEFT_16=3,20
PRIMER_RIGHT_16=441,24
PRIMER_LEFT_16_TM=61.285
PRIMER_RIGHT_16_TM=61.247
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_POSITION_PENALTY=60.000000
PRIMER_RIGHT_16_POSITION_PENALTY=0.000000
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=439
PRIMER_PAIR_16_PRODUCT_TM=75.9
PRIMER_PAIR_17_PENALTY=66.644794
PRIMER_LEFT_17_PENALTY=61.060283
PRIMER_RIGHT_17_PENALTY=5.584511
PRIMER_LEFT_17_SEQUENCE=GCGCTGAAAAGAATCCACA
PRIMER_RIGHT_17_SEQUENCE=TTACATCGCATACTTCTGAGTCC
PRIMER_LEFT_17=3,19
PRIMER_RIGHT_17=443,23
PRIMER_LEFT_17_TM=59.940
PRIMER_RIGHT_17_TM=58.415
PRIMER_LEFT_17_GC_PERCENT=47.368
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_POSITION_PENALTY=60.000000
PRIMER_RIGHT_17_POSITION_PENALTY=1.000000
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=441
PRIMER_PAIR_17_PRODUCT_TM=75.8
PRIMER_PAIR_18_PENALTY=66.864465
PRIMER_LEFT_18_PENALTY=63.147152
PRIMER_RIGHT_18_PENALTY=3.717312
PRIMER_LEFT_18_SEQUENCE=GCGCTGAAAAGAATCCACAAG
PRIMER_RIGHT_18_SEQUENCE=TACATCGCATACTTCTGAGTCCA
PRIMER_LEFT_18=3,21
PRIMER_RIGHT_18=442,23
PRIMER_LEFT_18_TM=62.147
PRIMER_RIGHT_18_TM=59.783
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_POSITION_PENALTY=60.000000
PRIMER_RIGHT_18_POSITION_PENALTY=0.500000
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=440
PRIMER_PAIR_18_PRODUCT_TM=75.9
PRIMER_PAIR_19_PENALTY=66.869403
PRIMER_LEFT_19_PENALTY=61.284892
PRIMER_RIGHT_19_PENALTY=5.584511
PRIMER_LEFT_19_SEQUENCE=GCGCTGAAAAGAATCCACAA
PRIMER_RIGHT_19_SEQUENCE=TTACATCGCATACTTCTGAGTCC
PRIMER_LEFT_19=3,20
PRIMER_RIGHT_19=443,23
PRIMER_LEFT_19_TM=61.285
PRIMER_RIGHT_19_TM=58.415
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_POSITION_PENALTY=60.000000
PRIMER_RIGHT_19_POSITION_PENALTY=1.000000
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=441
PRIMER_PAIR_19_PRODUCT_TM=75.8
=
SEQUENCE_ID=U51903/IQGAP2
SEQUENCE_START_CODON_POSITION=222
SEQUENCE_PRIMER=ATGCCACACGAAGAGCTGC
[encoded data, 5785 characters]
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 49099, GC content failed 2042, low tm 18100, high tm 10315, high end compl 16, long poly-x seq 226, high 3' stability 109, ok 18291
PRIMER_PAIR_EXPLAIN=considered 20, ok 20
PRIMER_STOP_CODON_POSITION=4947
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=10.109747
PRIMER_LEFT_0_PENALTY=3.573461
PRIMER_RIGHT_0_PENALTY=6.536286
PRIMER_LEFT_0_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_0_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGC
PRIMER_LEFT_0=222,19
PRIMER_RIGHT_0=4947,26
PRIMER_LEFT_0_TM=62.573
PRIMER_RIGHT_0_TM=60.536
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=38.462
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=4726
PRIMER_PAIR_0_PRODUCT_TM=76.9
PRIMER_PAIR_1_PENALTY=10.154611
PRIMER_LEFT_1_PENALTY=3.573461
PRIMER_RIGHT_1_PENALTY=6.581149
PRIMER_LEFT_1_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_1_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCA
PRIMER_LEFT_1=222,19
PRIMER_RIGHT_1=4948,25
PRIMER_LEFT_1_TM=62.573
PRIMER_RIGHT_1_TM=58.919
PRIMER_LEFT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_GC_PERCENT=36.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.500000
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=4727
PRIMER_PAIR_1_PRODUCT_TM=76.9
PRIMER_PAIR_2_PENALTY=11.102879
PRIMER_LEFT_2_PENALTY=3.573461
PRIMER_RIGHT_2_PENALTY=7.529417
PRIMER_LEFT_2_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTCA
PRIMER_LEFT_2=222,19
PRIMER_RIGHT_2=4949,26
PRIMER_LEFT_2_TM=62.573
PRIMER_RIGHT_2_TM=60.529
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=34.615
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=4728
PRIMER_PAIR_2_PRODUCT_TM=76.9
PRIMER_PAIR_3_PENALTY=11.479573
PRIMER_LEFT_3_PENALTY=3.573461
PRIMER_RIGHT_3_PENALTY=7.906112
PRIMER_LEFT_3_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_3_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTC
PRIMER_LEFT_3=222,19
PRIMER_RIGHT_3=4949,25
PRIMER_LEFT_3_TM=62.573
PRIMER_RIGHT_3_TM=58.094
PRIMER_LEFT_3_GC_PERCENT=57.895
PRIMER_RIGHT_3_GC_PERCENT=36.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=4728
PRIMER_PAIR_3_PRODUCT_TM=76.9
PRIMER_PAIR_4_PENALTY=11.733380
PRIMER_LEFT_4_PENALTY=3.573461
PRIMER_RIGHT_4_PENALTY=8.159919
PRIMER_LEFT_4_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_4_SEQUENCE=GGCACTTCACTTTCCATAGAACT
PRIMER_LEFT_4=222,19
PRIMER_RIGHT_4=4955,23
PRIMER_LEFT_4_TM=62.573
PRIMER_RIGHT_4_TM=58.840
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=4.000000
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=4734
PRIMER_PAIR_4_PRODUCT_TM=76.9
PRIMER_PAIR_5_PENALTY=11.773031
PRIMER_LEFT_5_PENALTY=3.573461
PRIMER_RIGHT_5_PENALTY=8.199569
PRIMER_LEFT_5_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_5_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTC
PRIMER_LEFT_5=222,19
PRIMER_RIGHT_5=4950,26
PRIMER_LEFT_5_TM=62.573
PRIMER_RIGHT_5_TM=59.300
PRIMER_LEFT_5_GC_PERCENT=57.895
PRIMER_RIGHT_5_GC_PERCENT=34.615
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=1.500000
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=4729
PRIMER_PAIR_5_PRODUCT_TM=76.9
PRIMER_PAIR_6_PENALTY=12.340390
PRIMER_LEFT_6_PENALTY=3.573461
PRIMER_RIGHT_6_PENALTY=8.766928
PRIMER_LEFT_6_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_6_SEQUENCE=AGGCACTTCACTTTCCATAGAACT
PRIMER_LEFT_6=222,19
PRIMER_RIGHT_6=4956,24
PRIMER_LEFT_6_TM=62.573
PRIMER_RIGHT_6_TM=59.733
PRIMER_LEFT_6_GC_PERCENT=57.895
PRIMER_RIGHT_6_GC_PERCENT=41.667
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=4.500000
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=4735
PRIMER_PAIR_6_PRODUCT_TM=76.9
PRIMER_PAIR_7_PENALTY=12.381077
PRIMER_LEFT_7_PENALTY=3.573461
PRIMER_RIGHT_7_PENALTY=8.807615
PRIMER_LEFT_7_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_7_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTT
PRIMER_LEFT_7=222,19
PRIMER_RIGHT_7=4950,25
PRIMER_LEFT_7_TM=62.573
PRIMER_RIGHT_7_TM=57.692
PRIMER_LEFT_7_GC_PERCENT=57.895
PRIMER_RIGHT_7_GC_PERCENT=32.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=1.500000
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=4729
PRIMER_PAIR_7_PRODUCT_TM=76.9
PRIMER_PAIR_8_PENALTY=12.659800
PRIMER_LEFT_8_PENALTY=3.573461
PRIMER_RIGHT_8_PENALTY=9.086339
PRIMER_LEFT_8_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_8_SEQUENCE=TCTGTAGGCACTTCACTTTCCA
PRIMER_LEFT_8=222,19
PRIMER_RIGHT_8=4961,22
PRIMER_LEFT_8_TM=62.573
PRIMER_RIGHT_8_TM=59.914
PRIMER_LEFT_8_GC_PERCENT=57.895
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=7.000000
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=4740
PRIMER_PAIR_8_PRODUCT_TM=76.9
PRIMER_PAIR_9_PENALTY=12.674341
PRIMER_LEFT_9_PENALTY=3.573461
PRIMER_RIGHT_9_PENALTY=9.100880
PRIMER_LEFT_9_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_9_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTTC
PRIMER_LEFT_9=222,19
PRIMER_RIGHT_9=4951,27
PRIMER_LEFT_9_TM=62.573
PRIMER_RIGHT_9_TM=60.101
PRIMER_LEFT_9_GC_PERCENT=57.895
PRIMER_RIGHT_9_GC_PERCENT=37.037
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=2.000000
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=4730
PRIMER_PAIR_9_PRODUCT_TM=76.9
PRIMER_PAIR_10_PENALTY=12.997281
PRIMER_LEFT_10_PENALTY=3.573461
PRIMER_RIGHT_10_PENALTY=9.423820
PRIMER_LEFT_10_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_10_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTT
PRIMER_LEFT_10=222,19
PRIMER_RIGHT_10=4951,26
PRIMER_LEFT_10_TM=62.573
PRIMER_RIGHT_10_TM=58.576
PRIMER_LEFT_10_GC_PERCENT=57.895
PRIMER_RIGHT_10_GC_PERCENT=34.615
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=4730
PRIMER_PAIR_10_PRODUCT_TM=76.9
PRIMER_PAIR_11_PENALTY=13.050739
PRIMER_LEFT_11_PENALTY=3.573461
PRIMER_RIGHT_11_PENALTY=9.477278
PRIMER_LEFT_11_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_11_SEQUENCE=CTGTAGGCACTTCACTTTCCA
PRIMER_LEFT_11=222,19
PRIMER_RIGHT_11=4960,21
PRIMER_LEFT_11_TM=62.573
PRIMER_RIGHT_11_TM=58.023
PRIMER_LEFT_11_GC_PERCENT=57.895
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=6.500000
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=4739
PRIMER_PAIR_11_PRODUCT_TM=76.9
PRIMER_PAIR_12_PENALTY=13.229460
PRIMER_LEFT_12_PENALTY=3.573461
PRIMER_RIGHT_12_PENALTY=9.655999
PRIMER_LEFT_12_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_12_SEQUENCE=TGTAGGCACTTCACTTTCCAT
PRIMER_LEFT_12=222,19
PRIMER_RIGHT_12=4959,21
PRIMER_LEFT_12_TM=62.573
PRIMER_RIGHT_12_TM=57.344
PRIMER_LEFT_12_GC_PERCENT=57.895
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=6.000000
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=4738
PRIMER_PAIR_12_PRODUCT_TM=76.9
PRIMER_PAIR_13_PENALTY=13.336123
PRIMER_LEFT_13_PENALTY=3.573461
PRIMER_RIGHT_13_PENALTY=9.762661
PRIMER_LEFT_13_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_13_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGCA
PRIMER_LEFT_13=222,19
PRIMER_RIGHT_13=4947,27
PRIMER_LEFT_13_TM=62.573
PRIMER_RIGHT_13_TM=62.763
PRIMER_LEFT_13_GC_PERCENT=57.895
PRIMER_RIGHT_13_GC_PERCENT=37.037
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=4726
PRIMER_PAIR_13_PRODUCT_TM=76.9
PRIMER_PAIR_14_PENALTY=13.671473
PRIMER_LEFT_14_PENALTY=3.573461
PRIMER_RIGHT_14_PENALTY=10.098012
PRIMER_LEFT_14_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_14_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTCA
PRIMER_LEFT_14=222,19
PRIMER_RIGHT_14=4950,27
PRIMER_LEFT_14_TM=62.573
PRIMER_RIGHT_14_TM=61.598
PRIMER_LEFT_14_GC_PERCENT=57.895
PRIMER_RIGHT_14_GC_PERCENT=33.333
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=1.500000
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=4729
PRIMER_PAIR_14_PRODUCT_TM=76.9
PRIMER_PAIR_15_PENALTY=13.678404
PRIMER_LEFT_15_PENALTY=3.573461
PRIMER_RIGHT_15_PENALTY=10.104943
PRIMER_LEFT_15_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_15_SEQUENCE=CTGTAGGCACTTCACTTTCCAT
PRIMER_LEFT_15=222,19
PRIMER_RIGHT_15=4960,22
PRIMER_LEFT_15_TM=62.573
PRIMER_RIGHT_15_TM=58.395
PRIMER_LEFT_15_GC_PERCENT=57.895
PRIMER_RIGHT_15_GC_PERCENT=45.455
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=6.500000
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=4739
PRIMER_PAIR_15_PRODUCT_TM=76.9
PRIMER_PAIR_16_PENALTY=13.751887
PRIMER_LEFT_16_PENALTY=3.573461
PRIMER_RIGHT_16_PENALTY=10.178426
PRIMER_LEFT_16_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_16_SEQUENCE=TCTGTAGGCACTTCACTTTCCAT
PRIMER_LEFT_16=222,19
PRIMER_RIGHT_16=4961,23
PRIMER_LEFT_16_TM=62.573
PRIMER_RIGHT_16_TM=60.178
PRIMER_LEFT_16_GC_PERCENT=57.895
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=7.000000
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=4740
PRIMER_PAIR_16_PRODUCT_TM=76.9
PRIMER_PAIR_17_PENALTY=13.790485
PRIMER_LEFT_17_PENALTY=3.573461
PRIMER_RIGHT_17_PENALTY=10.217023
PRIMER_LEFT_17_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_17_SEQUENCE=ACTTCACTTTCCATAGAACTTCTTGTT
PRIMER_LEFT_17=222,19
PRIMER_RIGHT_17=4952,27
PRIMER_LEFT_17_TM=62.573
PRIMER_RIGHT_17_TM=59.283
PRIMER_LEFT_17_GC_PERCENT=57.895
PRIMER_RIGHT_17_GC_PERCENT=33.333
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=2.500000
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=4731
PRIMER_PAIR_17_PRODUCT_TM=76.9
PRIMER_PAIR_18_PENALTY=13.822383
PRIMER_LEFT_18_PENALTY=3.573461
PRIMER_RIGHT_18_PENALTY=10.248922
PRIMER_LEFT_18_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_18_SEQUENCE=TAGGCACTTCACTTTCCATAGAACT
PRIMER_LEFT_18=222,19
PRIMER_RIGHT_18=4957,25
PRIMER_LEFT_18_TM=62.573
PRIMER_RIGHT_18_TM=59.751
PRIMER_LEFT_18_GC_PERCENT=57.895
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=5.000000
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=4736
PRIMER_PAIR_18_PRODUCT_TM=76.9
PRIMER_PAIR_19_PENALTY=13.836123
PRIMER_LEFT_19_PENALTY=3.573461
PRIMER_RIGHT_19_PENALTY=10.262661
PRIMER_LEFT_19_SEQUENCE=ATGCCACACGAAGAGCTGC
PRIMER_RIGHT_19_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCAGC
PRIMER_LEFT_19=222,19
PRIMER_RIGHT_19=4948,27
PRIMER_LEFT_19_TM=62.573
PRIMER_RIGHT_19_TM=62.763
PRIMER_LEFT_19_GC_PERCENT=57.895
PRIMER_RIGHT_19_GC_PERCENT=40.741
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=0.500000
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=4727
PRIMER_PAIR_19_PRODUCT_TM=76.9
=
SEQUENCE_ID=M59040/CD44
P3_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS_29..1114
SEQUENCE_START_CODON_POSITION=28
SEQUENCE_TEMPLATE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC
PRIMER_LEFT_EXPLAIN=considered 15178, would not amplify any of the ORF 11930, GC content failed 2, low tm 495, high tm 1516, high end compl 1, long poly-x seq 22, high 3' stability 27, ok 1185
PRIMER_RIGHT_EXPLAIN=considered 14630, GC content failed 362, low tm 3262, high tm 5355, high any compl 3, high end compl 2, long poly-x seq 73, high 3' stability 60, ok 5513
PRIMER_PAIR_EXPLAIN=considered 22, high end compl 1, ok 21
PRIMER_STOP_CODON_POSITION=1111
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=1.699596
PRIMER_LEFT_0_PENALTY=1.335843
PRIMER_RIGHT_0_PENALTY=0.363753
PRIMER_LEFT_0_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_0_SEQUENCE=ACACCCCAATCTTCATGTCC
PRIMER_LEFT_0=28,20
PRIMER_RIGHT_0=1111,20
PRIMER_LEFT_0_TM=61.336
PRIMER_RIGHT_0_TM=59.636
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=1084
PRIMER_PAIR_0_PRODUCT_TM=80.2
PRIMER_PAIR_1_PENALTY=2.962375
PRIMER_LEFT_1_PENALTY=2.598622
PRIMER_RIGHT_1_PENALTY=0.363753
PRIMER_LEFT_1_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_1_SEQUENCE=ACACCCCAATCTTCATGTCC
PRIMER_LEFT_1=28,19
PRIMER_RIGHT_1=1111,20
PRIMER_LEFT_1_TM=58.401
PRIMER_RIGHT_1_TM=59.636
PRIMER_LEFT_1_GC_PERCENT=47.368
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=1084
PRIMER_PAIR_1_PRODUCT_TM=80.2
PRIMER_PAIR_2_PENALTY=3.174248
PRIMER_LEFT_2_PENALTY=1.335843
PRIMER_RIGHT_2_PENALTY=1.838405
PRIMER_LEFT_2_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_2_SEQUENCE=TACACCCCAATCTTCATGTCC
PRIMER_LEFT_2=28,20
PRIMER_RIGHT_2=1112,21
PRIMER_LEFT_2_TM=61.336
PRIMER_RIGHT_2_TM=59.662
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.500000
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=1085
PRIMER_PAIR_2_PRODUCT_TM=80.1
PRIMER_PAIR_3_PENALTY=4.437027
PRIMER_LEFT_3_PENALTY=2.598622
PRIMER_RIGHT_3_PENALTY=1.838405
PRIMER_LEFT_3_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_3_SEQUENCE=TACACCCCAATCTTCATGTCC
PRIMER_LEFT_3=28,19
PRIMER_RIGHT_3=1112,21
PRIMER_LEFT_3_TM=58.401
PRIMER_RIGHT_3_TM=59.662
PRIMER_LEFT_3_GC_PERCENT=47.368
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.500000
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=1085
PRIMER_PAIR_3_PRODUCT_TM=80.1
PRIMER_PAIR_4_PENALTY=4.849987
PRIMER_LEFT_4_PENALTY=1.335843
PRIMER_RIGHT_4_PENALTY=3.514144
PRIMER_LEFT_4_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_4_SEQUENCE=ACACCCCAATCTTCATGTCCA
PRIMER_LEFT_4=28,20
PRIMER_RIGHT_4=1111,21
PRIMER_LEFT_4_TM=61.336
PRIMER_RIGHT_4_TM=62.514
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=1084
PRIMER_PAIR_4_PRODUCT_TM=80.2
PRIMER_PAIR_5_PENALTY=5.303759
PRIMER_LEFT_5_PENALTY=1.335843
PRIMER_RIGHT_5_PENALTY=3.967916
PRIMER_LEFT_5_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_5_SEQUENCE=TTACACCCCAATCTTCATGTCC
PRIMER_LEFT_5=28,20
PRIMER_RIGHT_5=1113,22
PRIMER_LEFT_5_TM=61.336
PRIMER_RIGHT_5_TM=60.968
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=1.000000
PRIMER_LEFT_5_END_STABILITY=10.0000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=1086
PRIMER_PAIR_5_PRODUCT_TM=80.1
PRIMER_PAIR_6_PENALTY=5.959170
PRIMER_LEFT_6_PENALTY=1.335843
PRIMER_RIGHT_6_PENALTY=4.623327
PRIMER_LEFT_6_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_6_SEQUENCE=TTACACCCCAATCTTCATGTC
PRIMER_LEFT_6=28,20
PRIMER_RIGHT_6=1113,21
PRIMER_LEFT_6_TM=61.336
PRIMER_RIGHT_6_TM=57.377
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
PRIMER_LEFT_6_END_STABILITY=10.0000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=1086
PRIMER_PAIR_6_PRODUCT_TM=80.1
PRIMER_PAIR_7_PENALTY=6.112765
PRIMER_LEFT_7_PENALTY=2.598622
PRIMER_RIGHT_7_PENALTY=3.514144
PRIMER_LEFT_7_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_7_SEQUENCE=ACACCCCAATCTTCATGTCCA
PRIMER_LEFT_7=28,19
PRIMER_RIGHT_7=1111,21
PRIMER_LEFT_7_TM=58.401
PRIMER_RIGHT_7_TM=62.514
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=1084
PRIMER_PAIR_7_PRODUCT_TM=80.2
PRIMER_PAIR_8_PENALTY=6.117351
PRIMER_LEFT_8_PENALTY=1.335843
PRIMER_RIGHT_8_PENALTY=4.781508
PRIMER_LEFT_8_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_8_SEQUENCE=TGTTACACCCCAATCTTCATGT
PRIMER_LEFT_8=28,20
PRIMER_RIGHT_8=1115,22
PRIMER_LEFT_8_TM=61.336
PRIMER_RIGHT_8_TM=59.218
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=2.000000
PRIMER_LEFT_8_END_STABILITY=10.0000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=1088
PRIMER_PAIR_8_PRODUCT_TM=80.1
PRIMER_PAIR_9_PENALTY=6.264997
PRIMER_LEFT_9_PENALTY=1.335843
PRIMER_RIGHT_9_PENALTY=4.929154
PRIMER_LEFT_9_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_9_SEQUENCE=TACACCCCAATCTTCATGTCCA
PRIMER_LEFT_9=28,20
PRIMER_RIGHT_9=1112,22
PRIMER_LEFT_9_TM=61.336
PRIMER_RIGHT_9_TM=62.429
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
PRIMER_LEFT_9_END_STABILITY=10.0000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=1085
PRIMER_PAIR_9_PRODUCT_TM=80.1
PRIMER_PAIR_10_PENALTY=6.542885
PRIMER_LEFT_10_PENALTY=1.335843
PRIMER_RIGHT_10_PENALTY=5.207042
PRIMER_LEFT_10_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_10_SEQUENCE=GTTACACCCCAATCTTCATGTC
PRIMER_LEFT_10=28,20
PRIMER_RIGHT_10=1114,22
PRIMER_LEFT_10_TM=61.336
PRIMER_RIGHT_10_TM=58.293
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.455
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=1.500000
PRIMER_LEFT_10_END_STABILITY=10.0000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=1087
PRIMER_PAIR_10_PRODUCT_TM=80.1
PRIMER_PAIR_11_PENALTY=6.566538
PRIMER_LEFT_11_PENALTY=2.598622
PRIMER_RIGHT_11_PENALTY=3.967916
PRIMER_LEFT_11_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_11_SEQUENCE=TTACACCCCAATCTTCATGTCC
PRIMER_LEFT_11=28,19
PRIMER_RIGHT_11=1113,22
PRIMER_LEFT_11_TM=58.401
PRIMER_RIGHT_11_TM=60.968
PRIMER_LEFT_11_GC_PERCENT=47.368
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=1.000000
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=1086
PRIMER_PAIR_11_PRODUCT_TM=80.1
PRIMER_PAIR_12_PENALTY=6.868587
PRIMER_LEFT_12_PENALTY=1.335843
PRIMER_RIGHT_12_PENALTY=5.532744
PRIMER_LEFT_12_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_12_SEQUENCE=GTGTTACACCCCAATCTTCATGT
PRIMER_LEFT_12=28,20
PRIMER_RIGHT_12=1116,23
PRIMER_LEFT_12_TM=61.336
PRIMER_RIGHT_12_TM=60.033
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=2.500000
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=1089
PRIMER_PAIR_12_PRODUCT_TM=80.1
PRIMER_PAIR_13_PENALTY=7.221949
PRIMER_LEFT_13_PENALTY=2.598622
PRIMER_RIGHT_13_PENALTY=4.623327
PRIMER_LEFT_13_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_13_SEQUENCE=TTACACCCCAATCTTCATGTC
PRIMER_LEFT_13=28,19
PRIMER_RIGHT_13=1113,21
PRIMER_LEFT_13_TM=58.401
PRIMER_RIGHT_13_TM=57.377
PRIMER_LEFT_13_GC_PERCENT=47.368
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=1.000000
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=1086
PRIMER_PAIR_13_PRODUCT_TM=80.1
PRIMER_PAIR_14_PENALTY=7.346131
PRIMER_LEFT_14_PENALTY=1.335843
PRIMER_RIGHT_14_PENALTY=6.010288
PRIMER_LEFT_14_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_14_SEQUENCE=TGTTACACCCCAATCTTCATGTC
PRIMER_LEFT_14=28,20
PRIMER_RIGHT_14=1115,23
PRIMER_LEFT_14_TM=61.336
PRIMER_RIGHT_14_TM=61.010
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=2.000000
PRIMER_LEFT_14_END_STABILITY=10.0000
PRIMER_RIGHT_14_END_STABILITY=6.3000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=1088
PRIMER_PAIR_14_PRODUCT_TM=80.1
PRIMER_PAIR_15_PENALTY=7.380130
PRIMER_LEFT_15_PENALTY=2.598622
PRIMER_RIGHT_15_PENALTY=4.781508
PRIMER_LEFT_15_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_15_SEQUENCE=TGTTACACCCCAATCTTCATGT
PRIMER_LEFT_15=28,19
PRIMER_RIGHT_15=1115,22
PRIMER_LEFT_15_TM=58.401
PRIMER_RIGHT_15_TM=59.218
PRIMER_LEFT_15_GC_PERCENT=47.368
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=2.000000
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=1088
PRIMER_PAIR_15_PRODUCT_TM=80.1
PRIMER_PAIR_16_PENALTY=7.527776
PRIMER_LEFT_16_PENALTY=2.598622
PRIMER_RIGHT_16_PENALTY=4.929154
PRIMER_LEFT_16_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_16_SEQUENCE=TACACCCCAATCTTCATGTCCA
PRIMER_LEFT_16=28,19
PRIMER_RIGHT_16=1112,22
PRIMER_LEFT_16_TM=58.401
PRIMER_RIGHT_16_TM=62.429
PRIMER_LEFT_16_GC_PERCENT=47.368
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=1085
PRIMER_PAIR_16_PRODUCT_TM=80.1
PRIMER_PAIR_17_PENALTY=7.536840
PRIMER_LEFT_17_PENALTY=1.335843
PRIMER_RIGHT_17_PENALTY=6.200997
PRIMER_LEFT_17_SEQUENCE=ATGGACAAGTTTTGGTGGCA
PRIMER_RIGHT_17_SEQUENCE=GTTACACCCCAATCTTCATGTCC
PRIMER_LEFT_17=28,20
PRIMER_RIGHT_17=1114,23
PRIMER_LEFT_17_TM=61.336
PRIMER_RIGHT_17_TM=61.701
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
PRIMER_LEFT_17_END_STABILITY=10.0000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=1087
PRIMER_PAIR_17_PRODUCT_TM=80.1
PRIMER_PAIR_18_PENALTY=7.805664
PRIMER_LEFT_18_PENALTY=2.598622
PRIMER_RIGHT_18_PENALTY=5.207042
PRIMER_LEFT_18_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_18_SEQUENCE=GTTACACCCCAATCTTCATGTC
PRIMER_LEFT_18=28,19
PRIMER_RIGHT_18=1114,22
PRIMER_LEFT_18_TM=58.401
PRIMER_RIGHT_18_TM=58.293
PRIMER_LEFT_18_GC_PERCENT=47.368
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=1.500000
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=1087
PRIMER_PAIR_18_PRODUCT_TM=80.1
PRIMER_PAIR_19_PENALTY=7.831834
PRIMER_LEFT_19_PENALTY=2.598622
PRIMER_RIGHT_19_PENALTY=5.233212
PRIMER_LEFT_19_SEQUENCE=ATGGACAAGTTTTGGTGGC
PRIMER_RIGHT_19_SEQUENCE=ACACCCCAATCTTCATGTCCAC
PRIMER_LEFT_19=28,19
PRIMER_RIGHT_19=1111,22
PRIMER_LEFT_19_TM=58.401
PRIMER_RIGHT_19_TM=63.233
PRIMER_LEFT_19_GC_PERCENT=47.368
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=0.000000
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=1084
PRIMER_PAIR_19_PRODUCT_TM=80.2
=
SEQUENCE_ID=M67454/FAS
P3_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS_195..1202
SEQUENCE_START_CODON_POSITION=194
SEQUENCE_TEMPLATE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG
PRIMER_LEFT_EXPLAIN=considered 21749, would not amplify any of the ORF 18372, GC content failed 10, low tm 984, high tm 1002, long poly-x seq 44, high 3' stability 21, ok 1316
PRIMER_RIGHT_EXPLAIN=considered 21076, GC content failed 1260, low tm 8619, high tm 3776, long poly-x seq 112, high 3' stability 48, ok 7261
PRIMER_PAIR_EXPLAIN=considered 21, ok 21
PRIMER_STOP_CODON_POSITION=1199
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=124.131340
PRIMER_LEFT_0_PENALTY=121.524367
PRIMER_RIGHT_0_PENALTY=2.606973
PRIMER_LEFT_0_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_0_SEQUENCE=AGACCAAGCTTTGGATTTCA
PRIMER_LEFT_0=200,19
PRIMER_RIGHT_0=1199,20
PRIMER_LEFT_0_TM=59.476
PRIMER_RIGHT_0_TM=57.393
PRIMER_LEFT_0_GC_PERCENT=63.158
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=120.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=1000
PRIMER_PAIR_0_PRODUCT_TM=76.4
PRIMER_PAIR_1_PENALTY=124.388123
PRIMER_LEFT_1_PENALTY=121.524367
PRIMER_RIGHT_1_PENALTY=2.863756
PRIMER_LEFT_1_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_1_SEQUENCE=AGACCAAGCTTTGGATTTCATT
PRIMER_LEFT_1=200,19
PRIMER_RIGHT_1=1199,22
PRIMER_LEFT_1_TM=59.476
PRIMER_RIGHT_1_TM=59.136
PRIMER_LEFT_1_GC_PERCENT=63.158
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_POSITION_PENALTY=120.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=1000
PRIMER_PAIR_1_PRODUCT_TM=76.4
PRIMER_PAIR_2_PENALTY=124.725988
PRIMER_LEFT_2_PENALTY=121.524367
PRIMER_RIGHT_2_PENALTY=3.201621
PRIMER_LEFT_2_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_2_SEQUENCE=AGACCAAGCTTTGGATTTCAT
PRIMER_LEFT_2=200,19
PRIMER_RIGHT_2=1199,21
PRIMER_LEFT_2_TM=59.476
PRIMER_RIGHT_2_TM=57.798
PRIMER_LEFT_2_GC_PERCENT=63.158
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_POSITION_PENALTY=120.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=1000
PRIMER_PAIR_2_PRODUCT_TM=76.4
PRIMER_PAIR_3_PENALTY=124.870439
PRIMER_LEFT_3_PENALTY=121.524367
PRIMER_RIGHT_3_PENALTY=3.346072
PRIMER_LEFT_3_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_3_SEQUENCE=AGACCAAGCTTTGGATTTCATTT
PRIMER_LEFT_3=200,19
PRIMER_RIGHT_3=1199,23
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=60.346
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_POSITION_PENALTY=120.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=1000
PRIMER_PAIR_3_PRODUCT_TM=76.4
PRIMER_PAIR_4_PENALTY=125.521988
PRIMER_LEFT_4_PENALTY=121.524367
PRIMER_RIGHT_4_PENALTY=3.997622
PRIMER_LEFT_4_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_4_SEQUENCE=TAGACCAAGCTTTGGATTTCA
PRIMER_LEFT_4=200,19
PRIMER_RIGHT_4=1200,21
PRIMER_LEFT_4_TM=59.476
PRIMER_RIGHT_4_TM=57.502
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_POSITION_PENALTY=120.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.500000
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=1001
PRIMER_PAIR_4_PRODUCT_TM=76.4
PRIMER_PAIR_5_PENALTY=125.689888
PRIMER_LEFT_5_PENALTY=123.082915
PRIMER_RIGHT_5_PENALTY=2.606973
PRIMER_LEFT_5_SEQUENCE=GGCATCTGGACCCTCCTACC
PRIMER_RIGHT_5_SEQUENCE=AGACCAAGCTTTGGATTTCA
PRIMER_LEFT_5=200,20
PRIMER_RIGHT_5=1199,20
PRIMER_LEFT_5_TM=63.083
PRIMER_RIGHT_5_TM=57.393
PRIMER_LEFT_5_GC_PERCENT=65.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_POSITION_PENALTY=120.000000
PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=1000
PRIMER_PAIR_5_PRODUCT_TM=76.4
PRIMER_PAIR_6_PENALTY=125.848946
PRIMER_LEFT_6_PENALTY=121.524367
PRIMER_RIGHT_6_PENALTY=4.324579
PRIMER_LEFT_6_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_6_SEQUENCE=TAGACCAAGCTTTGGATTTCATT
PRIMER_LEFT_6=200,19
PRIMER_RIGHT_6=1200,23
PRIMER_LEFT_6_TM=59.476
PRIMER_RIGHT_6_TM=59.175
PRIMER_LEFT_6_GC_PERCENT=63.158
PRIMER_RIGHT_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_POSITION_PENALTY=120.000000
PRIMER_RIGHT_6_POSITION_PENALTY=0.500000
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=1001
PRIMER_PAIR_6_PRODUCT_TM=76.4
PRIMER_PAIR_7_PENALTY=125.946671
PRIMER_LEFT_7_PENALTY=123.082915
PRIMER_RIGHT_7_PENALTY=2.863756
PRIMER_LEFT_7_SEQUENCE=GGCATCTGGACCCTCCTACC
PRIMER_RIGHT_7_SEQUENCE=AGACCAAGCTTTGGATTTCATT
PRIMER_LEFT_7=200,20
PRIMER_RIGHT_7=1199,22
PRIMER_LEFT_7_TM=63.083
PRIMER_RIGHT_7_TM=59.136
PRIMER_LEFT_7_GC_PERCENT=65.000
PRIMER_RIGHT_7_GC_PERCENT=36.364
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_POSITION_PENALTY=120.000000
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=1000
PRIMER_PAIR_7_PRODUCT_TM=76.4
PRIMER_PAIR_8_PENALTY=126.009466
PRIMER_LEFT_8_PENALTY=121.524367
PRIMER_RIGHT_8_PENALTY=4.485099
PRIMER_LEFT_8_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_8_SEQUENCE=CTAGACCAAGCTTTGGATTTCA
PRIMER_LEFT_8=200,19
PRIMER_RIGHT_8=1201,22
PRIMER_LEFT_8_TM=59.476
PRIMER_RIGHT_8_TM=58.515
PRIMER_LEFT_8_GC_PERCENT=63.158
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_POSITION_PENALTY=120.000000
PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=1002
PRIMER_PAIR_8_PRODUCT_TM=76.4
PRIMER_PAIR_9_PENALTY=126.136752
PRIMER_LEFT_9_PENALTY=121.524367
PRIMER_RIGHT_9_PENALTY=4.612386
PRIMER_LEFT_9_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_9_SEQUENCE=TAGACCAAGCTTTGGATTTCAT
PRIMER_LEFT_9=200,19
PRIMER_RIGHT_9=1200,22
PRIMER_LEFT_9_TM=59.476
PRIMER_RIGHT_9_TM=57.888
PRIMER_LEFT_9_GC_PERCENT=63.158
PRIMER_RIGHT_9_GC_PERCENT=36.364
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_POSITION_PENALTY=120.000000
PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=1001
PRIMER_PAIR_9_PRODUCT_TM=76.4
PRIMER_PAIR_10_PENALTY=126.267350
PRIMER_LEFT_10_PENALTY=121.524367
PRIMER_RIGHT_10_PENALTY=4.742983
PRIMER_LEFT_10_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_10_SEQUENCE=TCTAGACCAAGCTTTGGATTTCA
PRIMER_LEFT_10=200,19
PRIMER_RIGHT_10=1202,23
PRIMER_LEFT_10_TM=59.476
PRIMER_RIGHT_10_TM=60.243
PRIMER_LEFT_10_GC_PERCENT=63.158
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_POSITION_PENALTY=120.000000
PRIMER_RIGHT_10_POSITION_PENALTY=1.500000
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=1003
PRIMER_PAIR_10_PRODUCT_TM=76.4
PRIMER_PAIR_11_PENALTY=126.284536
PRIMER_LEFT_11_PENALTY=123.082915
PRIMER_RIGHT_11_PENALTY=3.201621
PRIMER_LEFT_11_SEQUENCE=GGCATCTGGACCCTCCTACC
PRIMER_RIGHT_11_SEQUENCE=AGACCAAGCTTTGGATTTCAT
PRIMER_LEFT_11=200,20
PRIMER_RIGHT_11=1199,21
PRIMER_LEFT_11_TM=63.083
PRIMER_RIGHT_11_TM=57.798
PRIMER_LEFT_11_GC_PERCENT=65.000
PRIMER_RIGHT_11_GC_PERCENT=38.095
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=120.000000
PRIMER_RIGHT_11_POSITION_PENALTY=0.000000
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=1000
PRIMER_PAIR_11_PRODUCT_TM=76.4
PRIMER_PAIR_12_PENALTY=126.368366
PRIMER_LEFT_12_PENALTY=121.524367
PRIMER_RIGHT_12_PENALTY=4.843999
PRIMER_LEFT_12_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_12_SEQUENCE=TAGACCAAGCTTTGGATTTCATTT
PRIMER_LEFT_12=200,19
PRIMER_RIGHT_12=1200,24
PRIMER_LEFT_12_TM=59.476
PRIMER_RIGHT_12_TM=60.344
PRIMER_LEFT_12_GC_PERCENT=63.158
PRIMER_RIGHT_12_GC_PERCENT=33.333
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_POSITION_PENALTY=120.000000
PRIMER_RIGHT_12_POSITION_PENALTY=0.500000
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=1001
PRIMER_PAIR_12_PRODUCT_TM=76.4
PRIMER_PAIR_13_PENALTY=126.428987
PRIMER_LEFT_13_PENALTY=123.082915
PRIMER_RIGHT_13_PENALTY=3.346072
PRIMER_LEFT_13_SEQUENCE=GGCATCTGGACCCTCCTACC
PRIMER_RIGHT_13_SEQUENCE=AGACCAAGCTTTGGATTTCATTT
PRIMER_LEFT_13=200,20
PRIMER_RIGHT_13=1199,23
PRIMER_LEFT_13_TM=63.083
PRIMER_RIGHT_13_TM=60.346
PRIMER_LEFT_13_GC_PERCENT=65.000
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_POSITION_PENALTY=120.000000
PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=7.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=1000
PRIMER_PAIR_13_PRODUCT_TM=76.4
PRIMER_PAIR_14_PENALTY=126.550645
PRIMER_LEFT_14_PENALTY=121.524367
PRIMER_RIGHT_14_PENALTY=5.026278
PRIMER_LEFT_14_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_14_SEQUENCE=CTAGACCAAGCTTTGGATTTCATT
PRIMER_LEFT_14=200,19
PRIMER_RIGHT_14=1201,24
PRIMER_LEFT_14_TM=59.476
PRIMER_RIGHT_14_TM=60.026
PRIMER_LEFT_14_GC_PERCENT=63.158
PRIMER_RIGHT_14_GC_PERCENT=37.500
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_POSITION_PENALTY=120.000000
PRIMER_RIGHT_14_POSITION_PENALTY=1.000000
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=1002
PRIMER_PAIR_14_PRODUCT_TM=76.4
PRIMER_PAIR_15_PENALTY=126.689801
PRIMER_LEFT_15_PENALTY=121.524367
PRIMER_RIGHT_15_PENALTY=5.165434
PRIMER_LEFT_15_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_15_SEQUENCE=CTAGACCAAGCTTTGGATTTCAT
PRIMER_LEFT_15=200,19
PRIMER_RIGHT_15=1201,23
PRIMER_LEFT_15_TM=59.476
PRIMER_RIGHT_15_TM=58.835
PRIMER_LEFT_15_GC_PERCENT=63.158
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=120.000000
PRIMER_RIGHT_15_POSITION_PENALTY=1.000000
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=1002
PRIMER_PAIR_15_PRODUCT_TM=76.4
PRIMER_PAIR_16_PENALTY=127.080537
PRIMER_LEFT_16_PENALTY=123.082915
PRIMER_RIGHT_16_PENALTY=3.997622
PRIMER_LEFT_16_SEQUENCE=GGCATCTGGACCCTCCTACC
PRIMER_RIGHT_16_SEQUENCE=TAGACCAAGCTTTGGATTTCA
PRIMER_LEFT_16=200,20
PRIMER_RIGHT_16=1200,21
PRIMER_LEFT_16_TM=63.083
PRIMER_RIGHT_16_TM=57.502
PRIMER_LEFT_16_GC_PERCENT=65.000
PRIMER_RIGHT_16_GC_PERCENT=38.095
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_POSITION_PENALTY=120.000000
PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=1001
PRIMER_PAIR_16_PRODUCT_TM=76.4
PRIMER_PAIR_17_PENALTY=127.389795
PRIMER_LEFT_17_PENALTY=121.524367
PRIMER_RIGHT_17_PENALTY=5.865428
PRIMER_LEFT_17_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_17_SEQUENCE=TCTAGACCAAGCTTTGGATTTC
PRIMER_LEFT_17=200,19
PRIMER_RIGHT_17=1202,22
PRIMER_LEFT_17_TM=59.476
PRIMER_RIGHT_17_TM=57.635
PRIMER_LEFT_17_GC_PERCENT=63.158
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_POSITION_PENALTY=120.000000
PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=1003
PRIMER_PAIR_17_PRODUCT_TM=76.4
PRIMER_PAIR_18_PENALTY=127.407494
PRIMER_LEFT_18_PENALTY=123.082915
PRIMER_RIGHT_18_PENALTY=4.324579
PRIMER_LEFT_18_SEQUENCE=GGCATCTGGACCCTCCTACC
PRIMER_RIGHT_18_SEQUENCE=TAGACCAAGCTTTGGATTTCATT
PRIMER_LEFT_18=200,20
PRIMER_RIGHT_18=1200,23
PRIMER_LEFT_18_TM=63.083
PRIMER_RIGHT_18_TM=59.175
PRIMER_LEFT_18_GC_PERCENT=65.000
PRIMER_RIGHT_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_POSITION_PENALTY=120.000000
PRIMER_RIGHT_18_POSITION_PENALTY=0.500000
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=1001
PRIMER_PAIR_18_PRODUCT_TM=76.4
PRIMER_PAIR_19_PENALTY=127.435780
PRIMER_LEFT_19_PENALTY=121.524367
PRIMER_RIGHT_19_PENALTY=5.911413
PRIMER_LEFT_19_SEQUENCE=GGCATCTGGACCCTCCTAC
PRIMER_RIGHT_19_SEQUENCE=AGACCAAGCTTTGGATTTCATTTC
PRIMER_LEFT_19=200,19
PRIMER_RIGHT_19=1199,24
PRIMER_LEFT_19_TM=59.476
PRIMER_RIGHT_19_TM=61.911
PRIMER_LEFT_19_GC_PERCENT=63.158
PRIMER_RIGHT_19_GC_PERCENT=37.500
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_POSITION_PENALTY=120.000000
PRIMER_RIGHT_19_POSITION_PENALTY=0.000000
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=1000
PRIMER_PAIR_19_PRODUCT_TM=76.4
=
SEQUENCE_ID=U33760/SKP1
P3_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS_94..585
SEQUENCE_START_CODON_POSITION=93
SEQUENCE_TEMPLATE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT
PRIMER_LEFT_EXPLAIN=considered 4706, would not amplify any of the ORF 3123, GC content failed 5, low tm 396, high tm 523, high end compl 7, long poly-x seq 20, high 3' stability 13, ok 619
PRIMER_RIGHT_EXPLAIN=considered 4645, GC content failed 11, low tm 1320, high tm 1167, high end compl 10, long poly-x seq 22, high 3' stability 16, ok 2099
PRIMER_PAIR_EXPLAIN=considered 21, ok 21
PRIMER_STOP_CODON_POSITION=582
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=12.352199
PRIMER_LEFT_0_PENALTY=9.051374
PRIMER_RIGHT_0_PENALTY=3.300825
PRIMER_LEFT_0_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_0_SEQUENCE=ACTTCTCTTCACACCACTGGTTC
PRIMER_LEFT_0=93,26
PRIMER_RIGHT_0=582,23
PRIMER_LEFT_0_TM=63.051
PRIMER_RIGHT_0_TM=59.699
PRIMER_LEFT_0_GC_PERCENT=38.462
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=490
PRIMER_PAIR_0_PRODUCT_TM=75.7
PRIMER_PAIR_1_PENALTY=13.663544
PRIMER_LEFT_1_PENALTY=9.051374
PRIMER_RIGHT_1_PENALTY=4.612170
PRIMER_LEFT_1_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_1_SEQUENCE=ACTTCTCTTCACACCACTGGTTCT
PRIMER_LEFT_1=93,26
PRIMER_RIGHT_1=582,24
PRIMER_LEFT_1_TM=63.051
PRIMER_RIGHT_1_TM=60.612
PRIMER_LEFT_1_GC_PERCENT=38.462
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=490
PRIMER_PAIR_1_PRODUCT_TM=75.7
PRIMER_PAIR_2_PENALTY=14.562250
PRIMER_LEFT_2_PENALTY=9.051374
PRIMER_RIGHT_2_PENALTY=5.510876
PRIMER_LEFT_2_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_2_SEQUENCE=TTCACTTCTCTTCACACCACTG
PRIMER_LEFT_2=93,26
PRIMER_RIGHT_2=585,22
PRIMER_LEFT_2_TM=63.051
PRIMER_RIGHT_2_TM=57.989
PRIMER_LEFT_2_GC_PERCENT=38.462
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=1.500000
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=493
PRIMER_PAIR_2_PRODUCT_TM=75.7
PRIMER_PAIR_3_PENALTY=14.622091
PRIMER_LEFT_3_PENALTY=9.051374
PRIMER_RIGHT_3_PENALTY=5.570716
PRIMER_LEFT_3_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_3_SEQUENCE=TTTCACTTCTCTTCACACCACTG
PRIMER_LEFT_3=93,26
PRIMER_RIGHT_3=586,23
PRIMER_LEFT_3_TM=63.051
PRIMER_RIGHT_3_TM=59.429
PRIMER_LEFT_3_GC_PERCENT=38.462
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=2.000000
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=494
PRIMER_PAIR_3_PRODUCT_TM=75.7
PRIMER_PAIR_4_PENALTY=15.830970
PRIMER_LEFT_4_PENALTY=9.051374
PRIMER_RIGHT_4_PENALTY=6.779595
PRIMER_LEFT_4_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_4_SEQUENCE=ATTTCACTTCTCTTCACACCACTG
PRIMER_LEFT_4=93,26
PRIMER_RIGHT_4=587,24
PRIMER_LEFT_4_TM=63.051
PRIMER_RIGHT_4_TM=59.720
PRIMER_LEFT_4_GC_PERCENT=38.462
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=2.500000
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=495
PRIMER_PAIR_4_PRODUCT_TM=75.7
PRIMER_PAIR_5_PENALTY=15.917260
PRIMER_LEFT_5_PENALTY=9.051374
PRIMER_RIGHT_5_PENALTY=6.865886
PRIMER_LEFT_5_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_5_SEQUENCE=CACTTCTCTTCACACCACTGGTTC
PRIMER_LEFT_5=93,26
PRIMER_RIGHT_5=583,24
PRIMER_LEFT_5_TM=63.051
PRIMER_RIGHT_5_TM=62.366
PRIMER_LEFT_5_GC_PERCENT=38.462
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=0.500000
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=491
PRIMER_PAIR_5_PRODUCT_TM=75.8
PRIMER_PAIR_6_PENALTY=16.195538
PRIMER_LEFT_6_PENALTY=9.051374
PRIMER_RIGHT_6_PENALTY=7.144164
PRIMER_LEFT_6_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_6_SEQUENCE=CATTTCACTTCTCTTCACACCA
PRIMER_LEFT_6=93,26
PRIMER_RIGHT_6=588,22
PRIMER_LEFT_6_TM=63.051
PRIMER_RIGHT_6_TM=57.856
PRIMER_LEFT_6_GC_PERCENT=38.462
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=496
PRIMER_PAIR_6_PRODUCT_TM=75.7
PRIMER_PAIR_7_PENALTY=16.293810
PRIMER_LEFT_7_PENALTY=9.051374
PRIMER_RIGHT_7_PENALTY=7.242436
PRIMER_LEFT_7_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_7_SEQUENCE=CATTTCACTTCTCTTCACACCAC
PRIMER_LEFT_7=93,26
PRIMER_RIGHT_7=588,23
PRIMER_LEFT_7_TM=63.051
PRIMER_RIGHT_7_TM=58.758
PRIMER_LEFT_7_GC_PERCENT=38.462
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=3.000000
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=496
PRIMER_PAIR_7_PRODUCT_TM=75.7
PRIMER_PAIR_8_PENALTY=16.329628
PRIMER_LEFT_8_PENALTY=9.051374
PRIMER_RIGHT_8_PENALTY=7.278253
PRIMER_LEFT_8_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_8_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTC
PRIMER_LEFT_8=93,26
PRIMER_RIGHT_8=582,25
PRIMER_LEFT_8_TM=63.051
PRIMER_RIGHT_8_TM=62.278
PRIMER_LEFT_8_GC_PERCENT=38.462
PRIMER_RIGHT_8_GC_PERCENT=48.000
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=0.000000
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=490
PRIMER_PAIR_8_PRODUCT_TM=75.7
PRIMER_PAIR_9_PENALTY=16.330970
PRIMER_LEFT_9_PENALTY=9.051374
PRIMER_RIGHT_9_PENALTY=7.279595
PRIMER_LEFT_9_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_9_SEQUENCE=CATTTCACTTCTCTTCACACCACT
PRIMER_LEFT_9=93,26
PRIMER_RIGHT_9=588,24
PRIMER_LEFT_9_TM=63.051
PRIMER_RIGHT_9_TM=59.720
PRIMER_LEFT_9_GC_PERCENT=38.462
PRIMER_RIGHT_9_GC_PERCENT=41.667
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=3.000000
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=496
PRIMER_PAIR_9_PRODUCT_TM=75.7
PRIMER_PAIR_10_PENALTY=16.793810
PRIMER_LEFT_10_PENALTY=9.051374
PRIMER_RIGHT_10_PENALTY=7.742436
PRIMER_LEFT_10_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_10_SEQUENCE=ACATTTCACTTCTCTTCACACCA
PRIMER_LEFT_10=93,26
PRIMER_RIGHT_10=589,23
PRIMER_LEFT_10_TM=63.051
PRIMER_RIGHT_10_TM=58.758
PRIMER_LEFT_10_GC_PERCENT=38.462
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=3.500000
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=497
PRIMER_PAIR_10_PRODUCT_TM=75.7
PRIMER_PAIR_11_PENALTY=16.959781
PRIMER_LEFT_11_PENALTY=9.051374
PRIMER_RIGHT_11_PENALTY=7.908407
PRIMER_LEFT_11_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_11_SEQUENCE=ACATTTCACTTCTCTTCACACCAC
PRIMER_LEFT_11=93,26
PRIMER_RIGHT_11=589,24
PRIMER_LEFT_11_TM=63.051
PRIMER_RIGHT_11_TM=59.592
PRIMER_LEFT_11_GC_PERCENT=38.462
PRIMER_RIGHT_11_GC_PERCENT=41.667
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=3.500000
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=497
PRIMER_PAIR_11_PRODUCT_TM=75.7
PRIMER_PAIR_12_PENALTY=17.126181
PRIMER_LEFT_12_PENALTY=9.051374
PRIMER_RIGHT_12_PENALTY=8.074807
PRIMER_LEFT_12_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_12_SEQUENCE=AACATTTCACTTCTCTTCACACCA
PRIMER_LEFT_12=93,26
PRIMER_RIGHT_12=590,24
PRIMER_LEFT_12_TM=63.051
PRIMER_RIGHT_12_TM=60.075
PRIMER_LEFT_12_GC_PERCENT=38.462
PRIMER_RIGHT_12_GC_PERCENT=37.500
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=4.000000
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=498
PRIMER_PAIR_12_PRODUCT_TM=75.7
PRIMER_PAIR_13_PENALTY=17.549645
PRIMER_LEFT_13_PENALTY=9.051374
PRIMER_RIGHT_13_PENALTY=8.498271
PRIMER_LEFT_13_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_13_SEQUENCE=ATTTCACTTCTCTTCACACCACT
PRIMER_LEFT_13=93,26
PRIMER_RIGHT_13=587,23
PRIMER_LEFT_13_TM=63.051
PRIMER_RIGHT_13_TM=57.002
PRIMER_LEFT_13_GC_PERCENT=38.462
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=8.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=2.500000
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=495
PRIMER_PAIR_13_PRODUCT_TM=75.7
PRIMER_PAIR_14_PENALTY=17.626181
PRIMER_LEFT_14_PENALTY=9.051374
PRIMER_RIGHT_14_PENALTY=8.574807
PRIMER_LEFT_14_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_14_SEQUENCE=CAACATTTCACTTCTCTTCACACC
PRIMER_LEFT_14=93,26
PRIMER_RIGHT_14=591,24
PRIMER_LEFT_14_TM=63.051
PRIMER_RIGHT_14_TM=60.075
PRIMER_LEFT_14_GC_PERCENT=38.462
PRIMER_RIGHT_14_GC_PERCENT=41.667
PRIMER_LEFT_14_SELF_ANY=8.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=4.500000
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=499
PRIMER_PAIR_14_PRODUCT_TM=75.7
PRIMER_PAIR_15_PENALTY=17.690498
PRIMER_LEFT_15_PENALTY=9.051374
PRIMER_RIGHT_15_PENALTY=8.639124
PRIMER_LEFT_15_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_15_SEQUENCE=CACTTCTCTTCACACCACTGGTTCT
PRIMER_LEFT_15=93,26
PRIMER_RIGHT_15=583,25
PRIMER_LEFT_15_TM=63.051
PRIMER_RIGHT_15_TM=63.139
PRIMER_LEFT_15_GC_PERCENT=38.462
PRIMER_RIGHT_15_GC_PERCENT=48.000
PRIMER_LEFT_15_SELF_ANY=8.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=0.500000
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=491
PRIMER_PAIR_15_PRODUCT_TM=75.8
PRIMER_PAIR_16_PENALTY=18.035208
PRIMER_LEFT_16_PENALTY=9.051374
PRIMER_RIGHT_16_PENALTY=8.983834
PRIMER_LEFT_16_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_16_SEQUENCE=ACATTTCACTTCTCTTCACACCACT
PRIMER_LEFT_16=93,26
PRIMER_RIGHT_16=589,25
PRIMER_LEFT_16_TM=63.051
PRIMER_RIGHT_16_TM=60.484
PRIMER_LEFT_16_GC_PERCENT=38.462
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=497
PRIMER_PAIR_16_PRODUCT_TM=75.7
PRIMER_PAIR_17_PENALTY=18.073566
PRIMER_LEFT_17_PENALTY=9.051374
PRIMER_RIGHT_17_PENALTY=9.022192
PRIMER_LEFT_17_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_17_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTCT
PRIMER_LEFT_17=93,26
PRIMER_RIGHT_17=582,26
PRIMER_LEFT_17_TM=63.051
PRIMER_RIGHT_17_TM=63.022
PRIMER_LEFT_17_GC_PERCENT=38.462
PRIMER_RIGHT_17_GC_PERCENT=46.154
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=0.000000
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=490
PRIMER_PAIR_17_PRODUCT_TM=75.7
PRIMER_PAIR_18_PENALTY=18.636182
PRIMER_LEFT_18_PENALTY=9.051374
PRIMER_RIGHT_18_PENALTY=9.584808
PRIMER_LEFT_18_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_18_SEQUENCE=GCACAACATTTCACTTCTCTTCA
PRIMER_LEFT_18=93,26
PRIMER_RIGHT_18=594,23
PRIMER_LEFT_18_TM=63.051
PRIMER_RIGHT_18_TM=59.415
PRIMER_LEFT_18_GC_PERCENT=38.462
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=8.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=6.000000
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=502
PRIMER_PAIR_18_PRODUCT_TM=75.8
PRIMER_PAIR_19_PENALTY=18.666052
PRIMER_LEFT_19_PENALTY=9.051374
PRIMER_RIGHT_19_PENALTY=9.614678
PRIMER_LEFT_19_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
PRIMER_RIGHT_19_SEQUENCE=AACATTTCACTTCTCTTCACACC
PRIMER_LEFT_19=93,26
PRIMER_RIGHT_19=590,23
PRIMER_LEFT_19_TM=63.051
PRIMER_RIGHT_19_TM=57.385
PRIMER_LEFT_19_GC_PERCENT=38.462
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=4.000000
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=498
PRIMER_PAIR_19_PRODUCT_TM=75.7
=
SEQUENCE_ID=X80343/P35
P3_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS_98..1021
SEQUENCE_START_CODON_POSITION=97
SEQUENCE_TEMPLATE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG
PRIMER_LEFT_EXPLAIN=considered 8968, would not amplify any of the ORF 6063, GC content failed 212, low tm 125, high tm 1996, high 3' stability 33, ok 539
PRIMER_RIGHT_EXPLAIN=considered 8912, GC content failed 682, low tm 430, high tm 6084, high end compl 14, high 3' stability 37, ok 1665
PRIMER_PAIR_EXPLAIN=considered 26, high end compl 4, ok 22
PRIMER_STOP_CODON_POSITION=1018
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=127.466882
PRIMER_LEFT_0_PENALTY=122.322207
PRIMER_RIGHT_0_PENALTY=5.144675
PRIMER_LEFT_0_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_0_SEQUENCE=TACAGTGCTCACCGATCCAG
PRIMER_LEFT_0=103,19
PRIMER_RIGHT_0=1028,20
PRIMER_LEFT_0_TM=61.322
PRIMER_RIGHT_0_TM=59.855
PRIMER_LEFT_0_GC_PERCENT=63.158
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_POSITION_PENALTY=120.000000
PRIMER_RIGHT_0_POSITION_PENALTY=5.000000
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=926
PRIMER_PAIR_0_PRODUCT_TM=84.4
PRIMER_PAIR_1_PENALTY=127.966882
PRIMER_LEFT_1_PENALTY=122.322207
PRIMER_RIGHT_1_PENALTY=5.644675
PRIMER_LEFT_1_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_1_SEQUENCE=CTACAGTGCTCACCGATCCA
PRIMER_LEFT_1=103,19
PRIMER_RIGHT_1=1029,20
PRIMER_LEFT_1_TM=61.322
PRIMER_RIGHT_1_TM=59.855
PRIMER_LEFT_1_GC_PERCENT=63.158
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_POSITION_PENALTY=120.000000
PRIMER_RIGHT_1_POSITION_PENALTY=5.500000
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=927
PRIMER_PAIR_1_PRODUCT_TM=84.4
PRIMER_PAIR_2_PENALTY=127.985635
PRIMER_LEFT_2_PENALTY=122.322207
PRIMER_RIGHT_2_PENALTY=5.663428
PRIMER_LEFT_2_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_2_SEQUENCE=ACAGTGCTCACCGATCCAG
PRIMER_LEFT_2=103,19
PRIMER_RIGHT_2=1027,19
PRIMER_LEFT_2_TM=61.322
PRIMER_RIGHT_2_TM=59.837
PRIMER_LEFT_2_GC_PERCENT=63.158
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_POSITION_PENALTY=120.000000
PRIMER_RIGHT_2_POSITION_PENALTY=4.500000
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=925
PRIMER_PAIR_2_PRODUCT_TM=84.4
PRIMER_PAIR_3_PENALTY=129.155466
PRIMER_LEFT_3_PENALTY=122.322207
PRIMER_RIGHT_3_PENALTY=6.833259
PRIMER_LEFT_3_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_3_SEQUENCE=GCTACAGTGCTCACCGATCC
PRIMER_LEFT_3=103,19
PRIMER_RIGHT_3=1030,20
PRIMER_LEFT_3_TM=61.322
PRIMER_RIGHT_3_TM=60.833
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_POSITION_PENALTY=120.000000
PRIMER_RIGHT_3_POSITION_PENALTY=6.000000
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=928
PRIMER_PAIR_3_PRODUCT_TM=84.4
PRIMER_PAIR_4_PENALTY=129.424588
PRIMER_LEFT_4_PENALTY=122.322207
PRIMER_RIGHT_4_PENALTY=7.102380
PRIMER_LEFT_4_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_4_SEQUENCE=AGGCTACAGTGCTCACCGAT
PRIMER_LEFT_4=103,19
PRIMER_RIGHT_4=1032,20
PRIMER_LEFT_4_TM=61.322
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_POSITION_PENALTY=120.000000
PRIMER_RIGHT_4_POSITION_PENALTY=7.000000
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=930
PRIMER_PAIR_4_PRODUCT_TM=84.4
PRIMER_PAIR_5_PENALTY=129.580280
PRIMER_LEFT_5_PENALTY=122.322207
PRIMER_RIGHT_5_PENALTY=7.258073
PRIMER_LEFT_5_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_5_SEQUENCE=TACAGTGCTCACCGATCCA
PRIMER_LEFT_5=103,19
PRIMER_RIGHT_5=1028,19
PRIMER_LEFT_5_TM=61.322
PRIMER_RIGHT_5_TM=58.742
PRIMER_LEFT_5_GC_PERCENT=63.158
PRIMER_RIGHT_5_GC_PERCENT=52.632
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_POSITION_PENALTY=120.000000
PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
PRIMER_LEFT_5_END_STABILITY=6.1000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=926
PRIMER_PAIR_5_PRODUCT_TM=84.4
PRIMER_PAIR_6_PENALTY=129.681344
PRIMER_LEFT_6_PENALTY=122.322207
PRIMER_RIGHT_6_PENALTY=7.359137
PRIMER_LEFT_6_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_6_SEQUENCE=CTACAGTGCTCACCGATCCAG
PRIMER_LEFT_6=103,19
PRIMER_RIGHT_6=1029,21
PRIMER_LEFT_6_TM=61.322
PRIMER_RIGHT_6_TM=60.859
PRIMER_LEFT_6_GC_PERCENT=63.158
PRIMER_RIGHT_6_GC_PERCENT=57.143
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_POSITION_PENALTY=120.000000
PRIMER_RIGHT_6_POSITION_PENALTY=5.500000
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=927
PRIMER_PAIR_6_PRODUCT_TM=84.4
PRIMER_PAIR_7_PENALTY=130.117964
PRIMER_LEFT_7_PENALTY=124.973289
PRIMER_RIGHT_7_PENALTY=5.144675
PRIMER_LEFT_7_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_7_SEQUENCE=TACAGTGCTCACCGATCCAG
PRIMER_LEFT_7=103,20
PRIMER_RIGHT_7=1028,20
PRIMER_LEFT_7_TM=64.973
PRIMER_RIGHT_7_TM=59.855
PRIMER_LEFT_7_GC_PERCENT=65.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_POSITION_PENALTY=120.000000
PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=926
PRIMER_PAIR_7_PRODUCT_TM=84.4
PRIMER_PAIR_8_PENALTY=130.602129
PRIMER_LEFT_8_PENALTY=122.322207
PRIMER_RIGHT_8_PENALTY=8.279921
PRIMER_LEFT_8_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_8_SEQUENCE=AGTGCTCACCGATCCAGGC
PRIMER_LEFT_8=103,19
PRIMER_RIGHT_8=1025,19
PRIMER_LEFT_8_TM=61.322
PRIMER_RIGHT_8_TM=63.780
PRIMER_LEFT_8_GC_PERCENT=63.158
PRIMER_RIGHT_8_GC_PERCENT=63.158
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_POSITION_PENALTY=120.000000
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=923
PRIMER_PAIR_8_PRODUCT_TM=84.4
PRIMER_PAIR_9_PENALTY=130.617964
PRIMER_LEFT_9_PENALTY=124.973289
PRIMER_RIGHT_9_PENALTY=5.644675
PRIMER_LEFT_9_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_9_SEQUENCE=CTACAGTGCTCACCGATCCA
PRIMER_LEFT_9=103,20
PRIMER_RIGHT_9=1029,20
PRIMER_LEFT_9_TM=64.973
PRIMER_RIGHT_9_TM=59.855
PRIMER_LEFT_9_GC_PERCENT=65.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_POSITION_PENALTY=120.000000
PRIMER_RIGHT_9_POSITION_PENALTY=5.500000
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=927
PRIMER_PAIR_9_PRODUCT_TM=84.4
PRIMER_PAIR_10_PENALTY=130.636717
PRIMER_LEFT_10_PENALTY=124.973289
PRIMER_RIGHT_10_PENALTY=5.663428
PRIMER_LEFT_10_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_10_SEQUENCE=ACAGTGCTCACCGATCCAG
PRIMER_LEFT_10=103,20
PRIMER_RIGHT_10=1027,19
PRIMER_LEFT_10_TM=64.973
PRIMER_RIGHT_10_TM=59.837
PRIMER_LEFT_10_GC_PERCENT=65.000
PRIMER_RIGHT_10_GC_PERCENT=57.895
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_POSITION_PENALTY=120.000000
PRIMER_RIGHT_10_POSITION_PENALTY=4.500000
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=925
PRIMER_PAIR_10_PRODUCT_TM=84.4
PRIMER_PAIR_11_PENALTY=130.735297
PRIMER_LEFT_11_PENALTY=122.322207
PRIMER_RIGHT_11_PENALTY=8.413090
PRIMER_LEFT_11_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_11_SEQUENCE=AGGCTACAGTGCTCACCGA
PRIMER_LEFT_11=103,19
PRIMER_RIGHT_11=1032,19
PRIMER_LEFT_11_TM=61.322
PRIMER_RIGHT_11_TM=59.587
PRIMER_LEFT_11_GC_PERCENT=63.158
PRIMER_RIGHT_11_GC_PERCENT=57.895
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=120.000000
PRIMER_RIGHT_11_POSITION_PENALTY=7.000000
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=930
PRIMER_PAIR_11_PRODUCT_TM=84.4
PRIMER_PAIR_12_PENALTY=130.977381
PRIMER_LEFT_12_PENALTY=122.322207
PRIMER_RIGHT_12_PENALTY=8.655174
PRIMER_LEFT_12_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_12_SEQUENCE=GGCTACAGTGCTCACCGAT
PRIMER_LEFT_12=103,19
PRIMER_RIGHT_12=1031,19
PRIMER_LEFT_12_TM=61.322
PRIMER_RIGHT_12_TM=58.845
PRIMER_LEFT_12_GC_PERCENT=63.158
PRIMER_RIGHT_12_GC_PERCENT=57.895
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=120.000000
PRIMER_RIGHT_12_POSITION_PENALTY=6.500000
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=9.8000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=929
PRIMER_PAIR_12_PRODUCT_TM=84.5
PRIMER_PAIR_13_PENALTY=131.729338
PRIMER_LEFT_13_PENALTY=122.322207
PRIMER_RIGHT_13_PENALTY=9.407131
PRIMER_LEFT_13_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_13_SEQUENCE=GCAGGCTACAGTGCTCACC
PRIMER_LEFT_13=103,19
PRIMER_RIGHT_13=1034,19
PRIMER_LEFT_13_TM=61.322
PRIMER_RIGHT_13_TM=59.593
PRIMER_LEFT_13_GC_PERCENT=63.158
PRIMER_RIGHT_13_GC_PERCENT=63.158
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_POSITION_PENALTY=120.000000
PRIMER_RIGHT_13_POSITION_PENALTY=8.000000
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=932
PRIMER_PAIR_13_PRODUCT_TM=84.5
PRIMER_PAIR_14_PENALTY=131.806548
PRIMER_LEFT_14_PENALTY=124.973289
PRIMER_RIGHT_14_PENALTY=6.833259
PRIMER_LEFT_14_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_14_SEQUENCE=GCTACAGTGCTCACCGATCC
PRIMER_LEFT_14=103,20
PRIMER_RIGHT_14=1030,20
PRIMER_LEFT_14_TM=64.973
PRIMER_RIGHT_14_TM=60.833
PRIMER_LEFT_14_GC_PERCENT=65.000
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_POSITION_PENALTY=120.000000
PRIMER_RIGHT_14_POSITION_PENALTY=6.000000
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=928
PRIMER_PAIR_14_PRODUCT_TM=84.4
PRIMER_PAIR_15_PENALTY=132.231362
PRIMER_LEFT_15_PENALTY=124.973289
PRIMER_RIGHT_15_PENALTY=7.258073
PRIMER_LEFT_15_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_15_SEQUENCE=TACAGTGCTCACCGATCCA
PRIMER_LEFT_15=103,20
PRIMER_RIGHT_15=1028,19
PRIMER_LEFT_15_TM=64.973
PRIMER_RIGHT_15_TM=58.742
PRIMER_LEFT_15_GC_PERCENT=65.000
PRIMER_RIGHT_15_GC_PERCENT=52.632
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_POSITION_PENALTY=120.000000
PRIMER_RIGHT_15_POSITION_PENALTY=5.000000
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=926
PRIMER_PAIR_15_PRODUCT_TM=84.4
PRIMER_PAIR_16_PENALTY=132.332426
PRIMER_LEFT_16_PENALTY=124.973289
PRIMER_RIGHT_16_PENALTY=7.359137
PRIMER_LEFT_16_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_16_SEQUENCE=CTACAGTGCTCACCGATCCAG
PRIMER_LEFT_16=103,20
PRIMER_RIGHT_16=1029,21
PRIMER_LEFT_16_TM=64.973
PRIMER_RIGHT_16_TM=60.859
PRIMER_LEFT_16_GC_PERCENT=65.000
PRIMER_RIGHT_16_GC_PERCENT=57.143
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_POSITION_PENALTY=120.000000
PRIMER_RIGHT_16_POSITION_PENALTY=5.500000
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=927
PRIMER_PAIR_16_PRODUCT_TM=84.4
PRIMER_PAIR_17_PENALTY=132.392649
PRIMER_LEFT_17_PENALTY=122.322207
PRIMER_RIGHT_17_PENALTY=10.070442
PRIMER_LEFT_17_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_17_SEQUENCE=CAGGCTACAGTGCTCACCGA
PRIMER_LEFT_17=103,19
PRIMER_RIGHT_17=1033,20
PRIMER_LEFT_17_TM=61.322
PRIMER_RIGHT_17_TM=62.570
PRIMER_LEFT_17_GC_PERCENT=63.158
PRIMER_RIGHT_17_GC_PERCENT=60.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_POSITION_PENALTY=120.000000
PRIMER_RIGHT_17_POSITION_PENALTY=7.500000
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=931
PRIMER_PAIR_17_PRODUCT_TM=84.4
PRIMER_PAIR_18_PENALTY=132.859566
PRIMER_LEFT_18_PENALTY=124.973289
PRIMER_RIGHT_18_PENALTY=7.886276
PRIMER_LEFT_18_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
PRIMER_RIGHT_18_SEQUENCE=CAGTGCTCACCGATCCAGG
PRIMER_LEFT_18=103,20
PRIMER_RIGHT_18=1026,19
PRIMER_LEFT_18_TM=64.973
PRIMER_RIGHT_18_TM=62.886
PRIMER_LEFT_18_GC_PERCENT=65.000
PRIMER_RIGHT_18_GC_PERCENT=63.158
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_POSITION_PENALTY=120.000000
PRIMER_RIGHT_18_POSITION_PENALTY=4.000000
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=924
PRIMER_PAIR_18_PRODUCT_TM=84.5
PRIMER_PAIR_19_PENALTY=132.985916
PRIMER_LEFT_19_PENALTY=122.322207
PRIMER_RIGHT_19_PENALTY=10.663709
PRIMER_LEFT_19_SEQUENCE=ACGGTGCTGTCCCTGTCTC
PRIMER_RIGHT_19_SEQUENCE=GCTACAGTGCTCACCGATCCA
PRIMER_LEFT_19=103,19
PRIMER_RIGHT_19=1030,21
PRIMER_LEFT_19_TM=61.322
PRIMER_RIGHT_19_TM=63.664
PRIMER_LEFT_19_GC_PERCENT=63.158
PRIMER_RIGHT_19_GC_PERCENT=57.143
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_POSITION_PENALTY=120.000000
PRIMER_RIGHT_19_POSITION_PENALTY=6.000000
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=928
PRIMER_PAIR_19_PRODUCT_TM=84.4
=
SEQUENCE_ID=L23959/DP1
P3_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS_38..1270
SEQUENCE_START_CODON_POSITION=37
SEQUENCE_TEMPLATE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA
PRIMER_LEFT_EXPLAIN=considered 11963, would not amplify any of the ORF 8309, GC content failed 29, low tm 476, high tm 2059, high 3' stability 47, ok 1043
PRIMER_RIGHT_EXPLAIN=considered 11769, GC content failed 99, low tm 1773, high tm 6322, long poly-x seq 25, high 3' stability 49, ok 3501
PRIMER_PAIR_EXPLAIN=considered 23, ok 23
PRIMER_STOP_CODON_POSITION=1267
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=4.447526
PRIMER_LEFT_0_PENALTY=4.039125
PRIMER_RIGHT_0_PENALTY=0.408401
PRIMER_LEFT_0_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_0_SEQUENCE=AGTCGTCCTCGTCATTCTCG
PRIMER_LEFT_0=37,20
PRIMER_RIGHT_0=1267,20
PRIMER_LEFT_0_TM=64.039
PRIMER_RIGHT_0_TM=60.408
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=1231
PRIMER_PAIR_0_PRODUCT_TM=81.3
PRIMER_PAIR_1_PENALTY=4.666064
PRIMER_LEFT_1_PENALTY=4.257663
PRIMER_RIGHT_1_PENALTY=0.408401
PRIMER_LEFT_1_SEQUENCE=ATGGCAAAAGATGCCGGTC
PRIMER_RIGHT_1_SEQUENCE=AGTCGTCCTCGTCATTCTCG
PRIMER_LEFT_1=37,19
PRIMER_RIGHT_1=1267,20
PRIMER_LEFT_1_TM=63.258
PRIMER_RIGHT_1_TM=60.408
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=1231
PRIMER_PAIR_1_PRODUCT_TM=81.3
PRIMER_PAIR_2_PENALTY=5.312234
PRIMER_LEFT_2_PENALTY=4.903833
PRIMER_RIGHT_2_PENALTY=0.408401
PRIMER_LEFT_2_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
PRIMER_RIGHT_2_SEQUENCE=AGTCGTCCTCGTCATTCTCG
PRIMER_LEFT_2=37,21
PRIMER_RIGHT_2=1267,20
PRIMER_LEFT_2_TM=63.904
PRIMER_RIGHT_2_TM=60.408
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=1231
PRIMER_PAIR_2_PRODUCT_TM=81.3
PRIMER_PAIR_3_PENALTY=6.160526
PRIMER_LEFT_3_PENALTY=4.039125
PRIMER_RIGHT_3_PENALTY=2.121401
PRIMER_LEFT_3_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_3_SEQUENCE=CAGTCGTCCTCGTCATTCTC
PRIMER_LEFT_3=37,20
PRIMER_RIGHT_3=1268,20
PRIMER_LEFT_3_TM=64.039
PRIMER_RIGHT_3_TM=58.379
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.500000
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=1232
PRIMER_PAIR_3_PRODUCT_TM=81.3
PRIMER_PAIR_4_PENALTY=6.274302
PRIMER_LEFT_4_PENALTY=4.039125
PRIMER_RIGHT_4_PENALTY=2.235177
PRIMER_LEFT_4_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_4_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
PRIMER_LEFT_4=37,20
PRIMER_RIGHT_4=1267,21
PRIMER_LEFT_4_TM=64.039
PRIMER_RIGHT_4_TM=61.235
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=1231
PRIMER_PAIR_4_PRODUCT_TM=81.3
PRIMER_PAIR_5_PENALTY=6.379064
PRIMER_LEFT_5_PENALTY=4.257663
PRIMER_RIGHT_5_PENALTY=2.121401
PRIMER_LEFT_5_SEQUENCE=ATGGCAAAAGATGCCGGTC
PRIMER_RIGHT_5_SEQUENCE=CAGTCGTCCTCGTCATTCTC
PRIMER_LEFT_5=37,19
PRIMER_RIGHT_5=1268,20
PRIMER_LEFT_5_TM=63.258
PRIMER_RIGHT_5_TM=58.379
PRIMER_LEFT_5_GC_PERCENT=52.632
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=0.500000
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=1232
PRIMER_PAIR_5_PRODUCT_TM=81.3
PRIMER_PAIR_6_PENALTY=6.440768
PRIMER_LEFT_6_PENALTY=4.039125
PRIMER_RIGHT_6_PENALTY=2.401642
PRIMER_LEFT_6_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_6_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
PRIMER_LEFT_6=37,20
PRIMER_RIGHT_6=1269,21
PRIMER_LEFT_6_TM=64.039
PRIMER_RIGHT_6_TM=60.402
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=1233
PRIMER_PAIR_6_PRODUCT_TM=81.3
PRIMER_PAIR_7_PENALTY=6.492840
PRIMER_LEFT_7_PENALTY=4.257663
PRIMER_RIGHT_7_PENALTY=2.235177
PRIMER_LEFT_7_SEQUENCE=ATGGCAAAAGATGCCGGTC
PRIMER_RIGHT_7_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
PRIMER_LEFT_7=37,19
PRIMER_RIGHT_7=1267,21
PRIMER_LEFT_7_TM=63.258
PRIMER_RIGHT_7_TM=61.235
PRIMER_LEFT_7_GC_PERCENT=52.632
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=1231
PRIMER_PAIR_7_PRODUCT_TM=81.3
PRIMER_PAIR_8_PENALTY=6.659306
PRIMER_LEFT_8_PENALTY=4.257663
PRIMER_RIGHT_8_PENALTY=2.401642
PRIMER_LEFT_8_SEQUENCE=ATGGCAAAAGATGCCGGTC
PRIMER_RIGHT_8_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
PRIMER_LEFT_8=37,19
PRIMER_RIGHT_8=1269,21
PRIMER_LEFT_8_TM=63.258
PRIMER_RIGHT_8_TM=60.402
PRIMER_LEFT_8_GC_PERCENT=52.632
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=1233
PRIMER_PAIR_8_PRODUCT_TM=81.3
PRIMER_PAIR_9_PENALTY=6.660526
PRIMER_LEFT_9_PENALTY=4.039125
PRIMER_RIGHT_9_PENALTY=2.621401
PRIMER_LEFT_9_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_9_SEQUENCE=TCAGTCGTCCTCGTCATTCT
PRIMER_LEFT_9=37,20
PRIMER_RIGHT_9=1269,20
PRIMER_LEFT_9_TM=64.039
PRIMER_RIGHT_9_TM=58.379
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=1.000000
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=1233
PRIMER_PAIR_9_PRODUCT_TM=81.3
PRIMER_PAIR_10_PENALTY=6.879064
PRIMER_LEFT_10_PENALTY=4.257663
PRIMER_RIGHT_10_PENALTY=2.621401
PRIMER_LEFT_10_SEQUENCE=ATGGCAAAAGATGCCGGTC
PRIMER_RIGHT_10_SEQUENCE=TCAGTCGTCCTCGTCATTCT
PRIMER_LEFT_10=37,19
PRIMER_RIGHT_10=1269,20
PRIMER_LEFT_10_TM=63.258
PRIMER_RIGHT_10_TM=58.379
PRIMER_LEFT_10_GC_PERCENT=52.632
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=1.000000
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=1233
PRIMER_PAIR_10_PRODUCT_TM=81.3
PRIMER_PAIR_11_PENALTY=7.025234
PRIMER_LEFT_11_PENALTY=4.903833
PRIMER_RIGHT_11_PENALTY=2.121401
PRIMER_LEFT_11_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
PRIMER_RIGHT_11_SEQUENCE=CAGTCGTCCTCGTCATTCTC
PRIMER_LEFT_11=37,21
PRIMER_RIGHT_11=1268,20
PRIMER_LEFT_11_TM=63.904
PRIMER_RIGHT_11_TM=58.379
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=0.500000
PRIMER_LEFT_11_END_STABILITY=5.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=1232
PRIMER_PAIR_11_PRODUCT_TM=81.3
PRIMER_PAIR_12_PENALTY=7.139009
PRIMER_LEFT_12_PENALTY=4.903833
PRIMER_RIGHT_12_PENALTY=2.235177
PRIMER_LEFT_12_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
PRIMER_RIGHT_12_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
PRIMER_LEFT_12=37,21
PRIMER_RIGHT_12=1267,21
PRIMER_LEFT_12_TM=63.904
PRIMER_RIGHT_12_TM=61.235
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=0.000000
PRIMER_LEFT_12_END_STABILITY=5.4000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=1231
PRIMER_PAIR_12_PRODUCT_TM=81.3
PRIMER_PAIR_13_PENALTY=7.202553
PRIMER_LEFT_13_PENALTY=4.039125
PRIMER_RIGHT_13_PENALTY=3.163428
PRIMER_LEFT_13_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_13_SEQUENCE=CGTCAGTCGTCCTCGTCAT
PRIMER_LEFT_13=37,20
PRIMER_RIGHT_13=1271,19
PRIMER_LEFT_13_TM=64.039
PRIMER_RIGHT_13_TM=59.837
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=57.895
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=2.000000
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=1235
PRIMER_PAIR_13_PRODUCT_TM=81.4
PRIMER_PAIR_14_PENALTY=7.219577
PRIMER_LEFT_14_PENALTY=4.039125
PRIMER_RIGHT_14_PENALTY=3.180452
PRIMER_LEFT_14_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_14_SEQUENCE=GTCAGTCGTCCTCGTCATTCT
PRIMER_LEFT_14=37,20
PRIMER_RIGHT_14=1270,21
PRIMER_LEFT_14_TM=64.039
PRIMER_RIGHT_14_TM=59.320
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=1.500000
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=1234
PRIMER_PAIR_14_PRODUCT_TM=81.3
PRIMER_PAIR_15_PENALTY=7.284820
PRIMER_LEFT_15_PENALTY=4.039125
PRIMER_RIGHT_15_PENALTY=3.245695
PRIMER_LEFT_15_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_15_SEQUENCE=ACGTCAGTCGTCCTCGTCAT
PRIMER_LEFT_15=37,20
PRIMER_RIGHT_15=1272,20
PRIMER_LEFT_15_TM=64.039
PRIMER_RIGHT_15_TM=60.746
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=2.500000
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=6.3000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=1236
PRIMER_PAIR_15_PRODUCT_TM=81.3
PRIMER_PAIR_16_PENALTY=7.305475
PRIMER_LEFT_16_PENALTY=4.903833
PRIMER_RIGHT_16_PENALTY=2.401642
PRIMER_LEFT_16_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
PRIMER_RIGHT_16_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
PRIMER_LEFT_16=37,21
PRIMER_RIGHT_16=1269,21
PRIMER_LEFT_16_TM=63.904
PRIMER_RIGHT_16_TM=60.402
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=1.000000
PRIMER_LEFT_16_END_STABILITY=5.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=1233
PRIMER_PAIR_16_PRODUCT_TM=81.3
PRIMER_PAIR_17_PENALTY=7.310448
PRIMER_LEFT_17_PENALTY=6.902047
PRIMER_RIGHT_17_PENALTY=0.408401
PRIMER_LEFT_17_SEQUENCE=ATGGCAAAAGATGCCGGTCTAA
PRIMER_RIGHT_17_SEQUENCE=AGTCGTCCTCGTCATTCTCG
PRIMER_LEFT_17=37,22
PRIMER_RIGHT_17=1267,20
PRIMER_LEFT_17_TM=64.902
PRIMER_RIGHT_17_TM=60.408
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=0.000000
PRIMER_LEFT_17_END_STABILITY=6.0000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=1231
PRIMER_PAIR_17_PRODUCT_TM=81.3
PRIMER_PAIR_18_PENALTY=7.318367
PRIMER_LEFT_18_PENALTY=4.039125
PRIMER_RIGHT_18_PENALTY=3.279241
PRIMER_LEFT_18_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_18_SEQUENCE=CGTCAGTCGTCCTCGTCATT
PRIMER_LEFT_18=37,20
PRIMER_RIGHT_18=1271,20
PRIMER_LEFT_18_TM=64.039
PRIMER_RIGHT_18_TM=61.279
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=2.000000
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=1235
PRIMER_PAIR_18_PRODUCT_TM=81.4
PRIMER_PAIR_19_PENALTY=7.322844
PRIMER_LEFT_19_PENALTY=4.039125
PRIMER_RIGHT_19_PENALTY=3.283719
PRIMER_LEFT_19_SEQUENCE=ATGGCAAAAGATGCCGGTCT
PRIMER_RIGHT_19_SEQUENCE=GTCAGTCGTCCTCGTCATTC
PRIMER_LEFT_19=37,20
PRIMER_RIGHT_19=1270,20
PRIMER_LEFT_19_TM=64.039
PRIMER_RIGHT_19_TM=58.216
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=1.500000
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=1234
PRIMER_PAIR_19_PRODUCT_TM=81.3
=
SEQUENCE_ID=X98032/E6AP
P3_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS_1..2559
SEQUENCE_START_CODON_POSITION=0
SEQUENCE_TEMPLATE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA
PRIMER_LEFT_EXPLAIN=considered 21507, would not amplify any of the ORF 14282, GC content failed 55, low tm 3071, high tm 1071, high end compl 6, long poly-x seq 102, high 3' stability 49, ok 2871
PRIMER_RIGHT_EXPLAIN=considered 21345, GC content failed 229, low tm 9108, high tm 3197, high end compl 7, long poly-x seq 106, high 3' stability 53, ok 8645
PRIMER_PAIR_EXPLAIN=considered 27, high end compl 6, ok 21
PRIMER_STOP_CODON_POSITION=2556
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=6.753748
PRIMER_LEFT_0_PENALTY=4.494664
PRIMER_RIGHT_0_PENALTY=2.259083
PRIMER_LEFT_0_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
PRIMER_RIGHT_0_SEQUENCE=TACAGCATGCCAAATCCTTT
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=2557,20
PRIMER_LEFT_0_TM=64.495
PRIMER_RIGHT_0_TM=58.241
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.500000
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=2558
PRIMER_PAIR_0_PRODUCT_TM=75.7
PRIMER_PAIR_1_PENALTY=6.894578
PRIMER_LEFT_1_PENALTY=4.494664
PRIMER_RIGHT_1_PENALTY=2.399914
PRIMER_LEFT_1_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
PRIMER_RIGHT_1_SEQUENCE=TTACAGCATGCCAAATCCTTT
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=2558,21
PRIMER_LEFT_1_TM=64.495
PRIMER_RIGHT_1_TM=59.600
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=1.000000
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=2559
PRIMER_PAIR_1_PRODUCT_TM=75.7
PRIMER_PAIR_2_PENALTY=7.253748
PRIMER_LEFT_2_PENALTY=4.494664
PRIMER_RIGHT_2_PENALTY=2.759083
PRIMER_LEFT_2_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
PRIMER_RIGHT_2_SEQUENCE=TTACAGCATGCCAAATCCTT
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=2558,20
PRIMER_LEFT_2_TM=64.495
PRIMER_RIGHT_2_TM=58.241
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=2559
PRIMER_PAIR_2_PRODUCT_TM=75.7
PRIMER_PAIR_3_PENALTY=7.335632
PRIMER_LEFT_3_PENALTY=4.494664
PRIMER_RIGHT_3_PENALTY=2.840968
PRIMER_LEFT_3_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
PRIMER_RIGHT_3_SEQUENCE=ACAGCATGCCAAATCCTTT
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=2556,19
PRIMER_LEFT_3_TM=64.495
PRIMER_RIGHT_3_TM=58.159
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.105
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=2557
PRIMER_PAIR_3_PRODUCT_TM=75.7
PRIMER_PAIR_4_PENALTY=127.102182
PRIMER_LEFT_4_PENALTY=124.343098
PRIMER_RIGHT_4_PENALTY=2.759083
PRIMER_LEFT_4_SEQUENCE=CGAGCAGCTGCAAAGCATC
PRIMER_RIGHT_4_SEQUENCE=TTACAGCATGCCAAATCCTT
PRIMER_LEFT_4=6,19
PRIMER_RIGHT_4=2558,20
PRIMER_LEFT_4_TM=63.343
PRIMER_RIGHT_4_TM=58.241
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_POSITION_PENALTY=120.000000
PRIMER_RIGHT_4_POSITION_PENALTY=1.000000
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=2553
PRIMER_PAIR_4_PRODUCT_TM=75.7
PRIMER_PAIR_5_PENALTY=127.764493
PRIMER_LEFT_5_PENALTY=125.005410
PRIMER_RIGHT_5_PENALTY=2.759083
PRIMER_LEFT_5_SEQUENCE=CGAGCAGCTGCAAAGCATCTA
PRIMER_RIGHT_5_SEQUENCE=TTACAGCATGCCAAATCCTT
PRIMER_LEFT_5=6,21
PRIMER_RIGHT_5=2558,20
PRIMER_LEFT_5_TM=64.005
PRIMER_RIGHT_5_TM=58.241
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_POSITION_PENALTY=120.000000
PRIMER_RIGHT_5_POSITION_PENALTY=1.000000
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=2553
PRIMER_PAIR_5_PRODUCT_TM=75.7
PRIMER_PAIR_6_PENALTY=131.139105
PRIMER_LEFT_6_PENALTY=128.880021
PRIMER_RIGHT_6_PENALTY=2.259083
PRIMER_LEFT_6_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_6_SEQUENCE=TACAGCATGCCAAATCCTTT
PRIMER_LEFT_6=6,24
PRIMER_RIGHT_6=2557,20
PRIMER_LEFT_6_TM=64.880
PRIMER_RIGHT_6_TM=58.241
PRIMER_LEFT_6_GC_PERCENT=45.833
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_POSITION_PENALTY=120.000000
PRIMER_RIGHT_6_POSITION_PENALTY=0.500000
PRIMER_LEFT_6_END_STABILITY=5.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=2552
PRIMER_PAIR_6_PRODUCT_TM=75.7
PRIMER_PAIR_7_PENALTY=131.279935
PRIMER_LEFT_7_PENALTY=128.880021
PRIMER_RIGHT_7_PENALTY=2.399914
PRIMER_LEFT_7_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_7_SEQUENCE=TTACAGCATGCCAAATCCTTT
PRIMER_LEFT_7=6,24
PRIMER_RIGHT_7=2558,21
PRIMER_LEFT_7_TM=64.880
PRIMER_RIGHT_7_TM=59.600
PRIMER_LEFT_7_GC_PERCENT=45.833
PRIMER_RIGHT_7_GC_PERCENT=38.095
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_POSITION_PENALTY=120.000000
PRIMER_RIGHT_7_POSITION_PENALTY=1.000000
PRIMER_LEFT_7_END_STABILITY=5.2000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=2553
PRIMER_PAIR_7_PRODUCT_TM=75.7
PRIMER_PAIR_8_PENALTY=131.639105
PRIMER_LEFT_8_PENALTY=128.880021
PRIMER_RIGHT_8_PENALTY=2.759083
PRIMER_LEFT_8_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_8_SEQUENCE=TTACAGCATGCCAAATCCTT
PRIMER_LEFT_8=6,24
PRIMER_RIGHT_8=2558,20
PRIMER_LEFT_8_TM=64.880
PRIMER_RIGHT_8_TM=58.241
PRIMER_LEFT_8_GC_PERCENT=45.833
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_POSITION_PENALTY=120.000000
PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
PRIMER_LEFT_8_END_STABILITY=5.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=2553
PRIMER_PAIR_8_PRODUCT_TM=75.7
PRIMER_PAIR_9_PENALTY=131.720989
PRIMER_LEFT_9_PENALTY=128.880021
PRIMER_RIGHT_9_PENALTY=2.840968
PRIMER_LEFT_9_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_9_SEQUENCE=ACAGCATGCCAAATCCTTT
PRIMER_LEFT_9=6,24
PRIMER_RIGHT_9=2556,19
PRIMER_LEFT_9_TM=64.880
PRIMER_RIGHT_9_TM=58.159
PRIMER_LEFT_9_GC_PERCENT=45.833
PRIMER_RIGHT_9_GC_PERCENT=42.105
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_POSITION_PENALTY=120.000000
PRIMER_RIGHT_9_POSITION_PENALTY=0.000000
PRIMER_LEFT_9_END_STABILITY=5.2000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=2551
PRIMER_PAIR_9_PRODUCT_TM=75.7
PRIMER_PAIR_10_PENALTY=183.964593
PRIMER_LEFT_10_PENALTY=181.705510
PRIMER_RIGHT_10_PENALTY=2.259083
PRIMER_LEFT_10_SEQUENCE=GCAGCTGCAAAGCATCTAAT
PRIMER_RIGHT_10_SEQUENCE=TACAGCATGCCAAATCCTTT
PRIMER_LEFT_10=9,20
PRIMER_RIGHT_10=2557,20
PRIMER_LEFT_10_TM=58.294
PRIMER_RIGHT_10_TM=58.241
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_POSITION_PENALTY=180.000000
PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=2549
PRIMER_PAIR_10_PRODUCT_TM=75.7
PRIMER_PAIR_11_PENALTY=184.105424
PRIMER_LEFT_11_PENALTY=181.705510
PRIMER_RIGHT_11_PENALTY=2.399914
PRIMER_LEFT_11_SEQUENCE=GCAGCTGCAAAGCATCTAAT
PRIMER_RIGHT_11_SEQUENCE=TTACAGCATGCCAAATCCTTT
PRIMER_LEFT_11=9,20
PRIMER_RIGHT_11=2558,21
PRIMER_LEFT_11_TM=58.294
PRIMER_RIGHT_11_TM=59.600
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=38.095
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_POSITION_PENALTY=180.000000
PRIMER_RIGHT_11_POSITION_PENALTY=1.000000
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=2550
PRIMER_PAIR_11_PRODUCT_TM=75.7
PRIMER_PAIR_12_PENALTY=184.464593
PRIMER_LEFT_12_PENALTY=181.705510
PRIMER_RIGHT_12_PENALTY=2.759083
PRIMER_LEFT_12_SEQUENCE=GCAGCTGCAAAGCATCTAAT
PRIMER_RIGHT_12_SEQUENCE=TTACAGCATGCCAAATCCTT
PRIMER_LEFT_12=9,20
PRIMER_RIGHT_12=2558,20
PRIMER_LEFT_12_TM=58.294
PRIMER_RIGHT_12_TM=58.241
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_POSITION_PENALTY=180.000000
PRIMER_RIGHT_12_POSITION_PENALTY=1.000000
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=2550
PRIMER_PAIR_12_PRODUCT_TM=75.7
PRIMER_PAIR_13_PENALTY=184.546477
PRIMER_LEFT_13_PENALTY=181.705510
PRIMER_RIGHT_13_PENALTY=2.840968
PRIMER_LEFT_13_SEQUENCE=GCAGCTGCAAAGCATCTAAT
PRIMER_RIGHT_13_SEQUENCE=ACAGCATGCCAAATCCTTT
PRIMER_LEFT_13=9,20
PRIMER_RIGHT_13=2556,19
PRIMER_LEFT_13_TM=58.294
PRIMER_RIGHT_13_TM=58.159
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.105
PRIMER_LEFT_13_SELF_ANY=8.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_POSITION_PENALTY=180.000000
PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
PRIMER_LEFT_13_END_STABILITY=5.9000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=2548
PRIMER_PAIR_13_PRODUCT_TM=75.7
PRIMER_PAIR_14_PENALTY=184.890860
PRIMER_LEFT_14_PENALTY=182.631776
PRIMER_RIGHT_14_PENALTY=2.259083
PRIMER_LEFT_14_SEQUENCE=GCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_14_SEQUENCE=TACAGCATGCCAAATCCTTT
PRIMER_LEFT_14=9,21
PRIMER_RIGHT_14=2557,20
PRIMER_LEFT_14_TM=58.368
PRIMER_RIGHT_14_TM=58.241
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=8.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_POSITION_PENALTY=180.000000
PRIMER_RIGHT_14_POSITION_PENALTY=0.500000
PRIMER_LEFT_14_END_STABILITY=5.2000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=2549
PRIMER_PAIR_14_PRODUCT_TM=75.7
PRIMER_PAIR_15_PENALTY=185.031690
PRIMER_LEFT_15_PENALTY=182.631776
PRIMER_RIGHT_15_PENALTY=2.399914
PRIMER_LEFT_15_SEQUENCE=GCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_15_SEQUENCE=TTACAGCATGCCAAATCCTTT
PRIMER_LEFT_15=9,21
PRIMER_RIGHT_15=2558,21
PRIMER_LEFT_15_TM=58.368
PRIMER_RIGHT_15_TM=59.600
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=38.095
PRIMER_LEFT_15_SELF_ANY=8.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_POSITION_PENALTY=180.000000
PRIMER_RIGHT_15_POSITION_PENALTY=1.000000
PRIMER_LEFT_15_END_STABILITY=5.2000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=2550
PRIMER_PAIR_15_PRODUCT_TM=75.7
PRIMER_PAIR_16_PENALTY=185.355825
PRIMER_LEFT_16_PENALTY=183.096742
PRIMER_RIGHT_16_PENALTY=2.259083
PRIMER_LEFT_16_SEQUENCE=GCAGCTGCAAAGCATCTAA
PRIMER_RIGHT_16_SEQUENCE=TACAGCATGCCAAATCCTTT
PRIMER_LEFT_16=9,19
PRIMER_RIGHT_16=2557,20
PRIMER_LEFT_16_TM=57.903
PRIMER_RIGHT_16_TM=58.241
PRIMER_LEFT_16_GC_PERCENT=47.368
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_POSITION_PENALTY=180.000000
PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
PRIMER_LEFT_16_END_STABILITY=6.0000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=2549
PRIMER_PAIR_16_PRODUCT_TM=75.7
PRIMER_PAIR_17_PENALTY=185.390860
PRIMER_LEFT_17_PENALTY=182.631776
PRIMER_RIGHT_17_PENALTY=2.759083
PRIMER_LEFT_17_SEQUENCE=GCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_17_SEQUENCE=TTACAGCATGCCAAATCCTT
PRIMER_LEFT_17=9,21
PRIMER_RIGHT_17=2558,20
PRIMER_LEFT_17_TM=58.368
PRIMER_RIGHT_17_TM=58.241
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_POSITION_PENALTY=180.000000
PRIMER_RIGHT_17_POSITION_PENALTY=1.000000
PRIMER_LEFT_17_END_STABILITY=5.2000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=2550
PRIMER_PAIR_17_PRODUCT_TM=75.7
PRIMER_PAIR_18_PENALTY=185.472744
PRIMER_LEFT_18_PENALTY=182.631776
PRIMER_RIGHT_18_PENALTY=2.840968
PRIMER_LEFT_18_SEQUENCE=GCAGCTGCAAAGCATCTAATA
PRIMER_RIGHT_18_SEQUENCE=ACAGCATGCCAAATCCTTT
PRIMER_LEFT_18=9,21
PRIMER_RIGHT_18=2556,19
PRIMER_LEFT_18_TM=58.368
PRIMER_RIGHT_18_TM=58.159
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=42.105
PRIMER_LEFT_18_SELF_ANY=8.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_POSITION_PENALTY=180.000000
PRIMER_RIGHT_18_POSITION_PENALTY=0.000000
PRIMER_LEFT_18_END_STABILITY=5.2000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=2548
PRIMER_PAIR_18_PRODUCT_TM=75.7
PRIMER_PAIR_19_PENALTY=185.496656
PRIMER_LEFT_19_PENALTY=183.096742
PRIMER_RIGHT_19_PENALTY=2.399914
PRIMER_LEFT_19_SEQUENCE=GCAGCTGCAAAGCATCTAA
PRIMER_RIGHT_19_SEQUENCE=TTACAGCATGCCAAATCCTTT
PRIMER_LEFT_19=9,19
PRIMER_RIGHT_19=2558,21
PRIMER_LEFT_19_TM=57.903
PRIMER_RIGHT_19_TM=59.600
PRIMER_LEFT_19_GC_PERCENT=47.368
PRIMER_RIGHT_19_GC_PERCENT=38.095
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_POSITION_PENALTY=180.000000
PRIMER_RIGHT_19_POSITION_PENALTY=1.000000
PRIMER_LEFT_19_END_STABILITY=6.0000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=2550
PRIMER_PAIR_19_PRODUCT_TM=75.7
=
SEQUENCE_ID=L22005/HCDC34
P3_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS_<1..897
SEQUENCE_START_CODON_POSITION=-3
SEQUENCE_TEMPLATE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT
PRIMER_LEFT_EXPLAIN=considered 11417, would not amplify any of the ORF 8815, GC content failed 428, low tm 104, high tm 1651, high end compl 2, long poly-x seq 18, high 3' stability 24, ok 375
PRIMER_RIGHT_EXPLAIN=considered 11034, GC content failed 1402, low tm 461, high tm 7364, high end compl 5, long poly-x seq 44, high 3' stability 50, ok 1708
PRIMER_PAIR_EXPLAIN=considered 23, high end compl 2, ok 21
PRIMER_STOP_CODON_POSITION=894
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=4027.917651
PRIMER_LEFT_0_PENALTY=4023.813185
PRIMER_RIGHT_0_PENALTY=4.104466
PRIMER_LEFT_0_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_0_SEQUENCE=GGTGTCAGGACTCCTCCGT
PRIMER_LEFT_0=198,19
PRIMER_RIGHT_0=900,19
PRIMER_LEFT_0_TM=62.813
PRIMER_RIGHT_0_TM=60.104
PRIMER_LEFT_0_GC_PERCENT=63.158
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_0_POSITION_PENALTY=3.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=703
PRIMER_PAIR_0_PRODUCT_TM=84.6
PRIMER_PAIR_1_PENALTY=4028.102281
PRIMER_LEFT_1_PENALTY=4023.813185
PRIMER_RIGHT_1_PENALTY=4.289096
PRIMER_LEFT_1_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_1_SEQUENCE=GTGTCAGGACTCCTCCGTG
PRIMER_LEFT_1=198,19
PRIMER_RIGHT_1=899,19
PRIMER_LEFT_1_TM=62.813
PRIMER_RIGHT_1_TM=59.211
PRIMER_LEFT_1_GC_PERCENT=63.158
PRIMER_RIGHT_1_GC_PERCENT=63.158
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_1_POSITION_PENALTY=2.500000
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=702
PRIMER_PAIR_1_PRODUCT_TM=84.6
PRIMER_PAIR_2_PENALTY=4028.107106
PRIMER_LEFT_2_PENALTY=4024.002640
PRIMER_RIGHT_2_PENALTY=4.104466
PRIMER_LEFT_2_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_2_SEQUENCE=GGTGTCAGGACTCCTCCGT
PRIMER_LEFT_2=198,20
PRIMER_RIGHT_2=900,19
PRIMER_LEFT_2_TM=64.003
PRIMER_RIGHT_2_TM=60.104
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=63.158
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_2_POSITION_PENALTY=3.000000
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=703
PRIMER_PAIR_2_PRODUCT_TM=84.6
PRIMER_PAIR_3_PENALTY=4028.179180
PRIMER_LEFT_3_PENALTY=4023.813185
PRIMER_RIGHT_3_PENALTY=4.365996
PRIMER_LEFT_3_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_3_SEQUENCE=AGGACTCCTCCGTGCCAGA
PRIMER_LEFT_3=198,19
PRIMER_RIGHT_3=894,19
PRIMER_LEFT_3_TM=62.813
PRIMER_RIGHT_3_TM=63.366
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=697
PRIMER_PAIR_3_PRODUCT_TM=84.6
PRIMER_PAIR_4_PENALTY=4028.291736
PRIMER_LEFT_4_PENALTY=4024.002640
PRIMER_RIGHT_4_PENALTY=4.289096
PRIMER_LEFT_4_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_4_SEQUENCE=GTGTCAGGACTCCTCCGTG
PRIMER_LEFT_4=198,20
PRIMER_RIGHT_4=899,19
PRIMER_LEFT_4_TM=64.003
PRIMER_RIGHT_4_TM=59.211
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_4_POSITION_PENALTY=2.500000
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=702
PRIMER_PAIR_4_PRODUCT_TM=84.6
PRIMER_PAIR_5_PENALTY=4028.368636
PRIMER_LEFT_5_PENALTY=4024.002640
PRIMER_RIGHT_5_PENALTY=4.365996
PRIMER_LEFT_5_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_5_SEQUENCE=AGGACTCCTCCGTGCCAGA
PRIMER_LEFT_5=198,20
PRIMER_RIGHT_5=894,19
PRIMER_LEFT_5_TM=64.003
PRIMER_RIGHT_5_TM=63.366
PRIMER_LEFT_5_GC_PERCENT=60.000
PRIMER_RIGHT_5_GC_PERCENT=63.158
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=697
PRIMER_PAIR_5_PRODUCT_TM=84.6
PRIMER_PAIR_6_PENALTY=4029.302304
PRIMER_LEFT_6_PENALTY=4023.813185
PRIMER_RIGHT_6_PENALTY=5.489119
PRIMER_LEFT_6_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_6_SEQUENCE=TGTCAGGACTCCTCCGTGC
PRIMER_LEFT_6=198,19
PRIMER_RIGHT_6=898,19
PRIMER_LEFT_6_TM=62.813
PRIMER_RIGHT_6_TM=62.489
PRIMER_LEFT_6_GC_PERCENT=63.158
PRIMER_RIGHT_6_GC_PERCENT=63.158
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_6_POSITION_PENALTY=2.000000
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=701
PRIMER_PAIR_6_PRODUCT_TM=84.5
PRIMER_PAIR_7_PENALTY=4029.563238
PRIMER_LEFT_7_PENALTY=4023.813185
PRIMER_RIGHT_7_PENALTY=5.750053
PRIMER_LEFT_7_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_7_SEQUENCE=GTCAGGACTCCTCCGTGCC
PRIMER_LEFT_7=198,19
PRIMER_RIGHT_7=897,19
PRIMER_LEFT_7_TM=62.813
PRIMER_RIGHT_7_TM=63.250
PRIMER_LEFT_7_GC_PERCENT=63.158
PRIMER_RIGHT_7_GC_PERCENT=68.421
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_7_POSITION_PENALTY=1.500000
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=700
PRIMER_PAIR_7_PRODUCT_TM=84.6
PRIMER_PAIR_8_PENALTY=4029.590879
PRIMER_LEFT_8_PENALTY=4023.813185
PRIMER_RIGHT_8_PENALTY=5.777694
PRIMER_LEFT_8_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_8_SEQUENCE=GTGTCAGGACTCCTCCGTGC
PRIMER_LEFT_8=198,19
PRIMER_RIGHT_8=899,20
PRIMER_LEFT_8_TM=62.813
PRIMER_RIGHT_8_TM=63.278
PRIMER_LEFT_8_GC_PERCENT=63.158
PRIMER_RIGHT_8_GC_PERCENT=65.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_8_POSITION_PENALTY=2.500000
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=9.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=702
PRIMER_PAIR_8_PRODUCT_TM=84.6
PRIMER_PAIR_9_PENALTY=4029.657883
PRIMER_LEFT_9_PENALTY=4023.813185
PRIMER_RIGHT_9_PENALTY=5.844698
PRIMER_LEFT_9_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_9_SEQUENCE=AGGACTCCTCCGTGCCAGAGT
PRIMER_LEFT_9=198,19
PRIMER_RIGHT_9=894,21
PRIMER_LEFT_9_TM=62.813
PRIMER_RIGHT_9_TM=64.845
PRIMER_LEFT_9_GC_PERCENT=63.158
PRIMER_RIGHT_9_GC_PERCENT=61.905
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_9_POSITION_PENALTY=0.000000
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=697
PRIMER_PAIR_9_PRODUCT_TM=84.6
PRIMER_PAIR_10_PENALTY=4029.703903
PRIMER_LEFT_10_PENALTY=4023.813185
PRIMER_RIGHT_10_PENALTY=5.890719
PRIMER_LEFT_10_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_10_SEQUENCE=CAGGACTCCTCCGTGCCAG
PRIMER_LEFT_10=198,19
PRIMER_RIGHT_10=895,19
PRIMER_LEFT_10_TM=62.813
PRIMER_RIGHT_10_TM=64.391
PRIMER_LEFT_10_GC_PERCENT=63.158
PRIMER_RIGHT_10_GC_PERCENT=68.421
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=698
PRIMER_PAIR_10_PRODUCT_TM=84.6
PRIMER_PAIR_11_PENALTY=4029.752693
PRIMER_LEFT_11_PENALTY=4024.002640
PRIMER_RIGHT_11_PENALTY=5.750053
PRIMER_LEFT_11_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_11_SEQUENCE=GTCAGGACTCCTCCGTGCC
PRIMER_LEFT_11=198,20
PRIMER_RIGHT_11=897,19
PRIMER_LEFT_11_TM=64.003
PRIMER_RIGHT_11_TM=63.250
PRIMER_LEFT_11_GC_PERCENT=60.000
PRIMER_RIGHT_11_GC_PERCENT=68.421
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_11_POSITION_PENALTY=1.500000
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=700
PRIMER_PAIR_11_PRODUCT_TM=84.6
PRIMER_PAIR_12_PENALTY=4029.847338
PRIMER_LEFT_12_PENALTY=4024.002640
PRIMER_RIGHT_12_PENALTY=5.844698
PRIMER_LEFT_12_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_12_SEQUENCE=AGGACTCCTCCGTGCCAGAGT
PRIMER_LEFT_12=198,20
PRIMER_RIGHT_12=894,21
PRIMER_LEFT_12_TM=64.003
PRIMER_RIGHT_12_TM=64.845
PRIMER_LEFT_12_GC_PERCENT=60.000
PRIMER_RIGHT_12_GC_PERCENT=61.905
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_12_POSITION_PENALTY=0.000000
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=697
PRIMER_PAIR_12_PRODUCT_TM=84.6
PRIMER_PAIR_13_PENALTY=4029.893359
PRIMER_LEFT_13_PENALTY=4024.002640
PRIMER_RIGHT_13_PENALTY=5.890719
PRIMER_LEFT_13_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_13_SEQUENCE=CAGGACTCCTCCGTGCCAG
PRIMER_LEFT_13=198,20
PRIMER_RIGHT_13=895,19
PRIMER_LEFT_13_TM=64.003
PRIMER_RIGHT_13_TM=64.391
PRIMER_LEFT_13_GC_PERCENT=60.000
PRIMER_RIGHT_13_GC_PERCENT=68.421
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_13_POSITION_PENALTY=0.500000
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=698
PRIMER_PAIR_13_PRODUCT_TM=84.6
PRIMER_PAIR_14_PENALTY=4029.932546
PRIMER_LEFT_14_PENALTY=4023.813185
PRIMER_RIGHT_14_PENALTY=6.119361
PRIMER_LEFT_14_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_14_SEQUENCE=GGTGTCAGGACTCCTCCGTG
PRIMER_LEFT_14=198,19
PRIMER_RIGHT_14=900,20
PRIMER_LEFT_14_TM=62.813
PRIMER_RIGHT_14_TM=63.119
PRIMER_LEFT_14_GC_PERCENT=63.158
PRIMER_RIGHT_14_GC_PERCENT=65.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_14_POSITION_PENALTY=3.000000
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=703
PRIMER_PAIR_14_PRODUCT_TM=84.6
PRIMER_PAIR_15_PENALTY=4030.122001
PRIMER_LEFT_15_PENALTY=4024.002640
PRIMER_RIGHT_15_PENALTY=6.119361
PRIMER_LEFT_15_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_15_SEQUENCE=GGTGTCAGGACTCCTCCGTG
PRIMER_LEFT_15=198,20
PRIMER_RIGHT_15=900,20
PRIMER_LEFT_15_TM=64.003
PRIMER_RIGHT_15_TM=63.119
PRIMER_LEFT_15_GC_PERCENT=60.000
PRIMER_RIGHT_15_GC_PERCENT=65.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_15_POSITION_PENALTY=3.000000
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=703
PRIMER_PAIR_15_PRODUCT_TM=84.6
PRIMER_PAIR_16_PENALTY=4030.432546
PRIMER_LEFT_16_PENALTY=4023.813185
PRIMER_RIGHT_16_PENALTY=6.619361
PRIMER_LEFT_16_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_16_SEQUENCE=TGGTGTCAGGACTCCTCCGT
PRIMER_LEFT_16=198,19
PRIMER_RIGHT_16=901,20
PRIMER_LEFT_16_TM=62.813
PRIMER_RIGHT_16_TM=63.119
PRIMER_LEFT_16_GC_PERCENT=63.158
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=704
PRIMER_PAIR_16_PRODUCT_TM=84.6
PRIMER_PAIR_17_PENALTY=4030.622001
PRIMER_LEFT_17_PENALTY=4024.002640
PRIMER_RIGHT_17_PENALTY=6.619361
PRIMER_LEFT_17_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_17_SEQUENCE=TGGTGTCAGGACTCCTCCGT
PRIMER_LEFT_17=198,20
PRIMER_RIGHT_17=901,20
PRIMER_LEFT_17_TM=64.003
PRIMER_RIGHT_17_TM=63.119
PRIMER_LEFT_17_GC_PERCENT=60.000
PRIMER_RIGHT_17_GC_PERCENT=60.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_17_POSITION_PENALTY=3.500000
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=704
PRIMER_PAIR_17_PRODUCT_TM=84.6
PRIMER_PAIR_18_PENALTY=4030.636711
PRIMER_LEFT_18_PENALTY=4023.813185
PRIMER_RIGHT_18_PENALTY=6.823526
PRIMER_LEFT_18_SEQUENCE=CTAGTGCCCAGCTCGCAGA
PRIMER_RIGHT_18_SEQUENCE=TGGTGTCAGGACTCCTCCG
PRIMER_LEFT_18=198,19
PRIMER_RIGHT_18=901,19
PRIMER_LEFT_18_TM=62.813
PRIMER_RIGHT_18_TM=62.324
PRIMER_LEFT_18_GC_PERCENT=63.158
PRIMER_RIGHT_18_GC_PERCENT=63.158
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_18_POSITION_PENALTY=3.500000
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=704
PRIMER_PAIR_18_PRODUCT_TM=84.6
PRIMER_PAIR_19_PENALTY=4030.826166
PRIMER_LEFT_19_PENALTY=4024.002640
PRIMER_RIGHT_19_PENALTY=6.823526
PRIMER_LEFT_19_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
PRIMER_RIGHT_19_SEQUENCE=TGGTGTCAGGACTCCTCCG
PRIMER_LEFT_19=198,20
PRIMER_RIGHT_19=901,19
PRIMER_LEFT_19_TM=64.003
PRIMER_RIGHT_19_TM=62.324
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=63.158
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_POSITION_PENALTY=4020.000000
PRIMER_RIGHT_19_POSITION_PENALTY=3.500000
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=704
PRIMER_PAIR_19_PRODUCT_TM=84.6
=
SEQUENCE_ID=M24194/MHC/RACK1
P3_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS_96..1049
SEQUENCE_START_CODON_POSITION=95
SEQUENCE_TEMPLATE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC
PRIMER_LEFT_EXPLAIN=considered 8997, would not amplify any of the ORF 6119, GC content failed 1, low tm 301, high tm 1735, high 3' stability 24, ok 817
PRIMER_RIGHT_EXPLAIN=considered 8758, GC content failed 64, low tm 957, high tm 5153, high end compl 7, long poly-x seq 16, high 3' stability 45, ok 2516
PRIMER_PAIR_EXPLAIN=considered 38, high end compl 14, ok 24
PRIMER_STOP_CODON_POSITION=1046
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=5.438070
PRIMER_LEFT_0_PENALTY=2.658657
PRIMER_RIGHT_0_PENALTY=2.779412
PRIMER_LEFT_0_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_0_SEQUENCE=TCTAGCGTGTGCCAATGGT
PRIMER_LEFT_0=95,22
PRIMER_RIGHT_0=1049,19
PRIMER_LEFT_0_TM=60.659
PRIMER_RIGHT_0_TM=60.279
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=52.632
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=1.500000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=955
PRIMER_PAIR_0_PRODUCT_TM=81.7
PRIMER_PAIR_1_PENALTY=5.568198
PRIMER_LEFT_1_PENALTY=2.788786
PRIMER_RIGHT_1_PENALTY=2.779412
PRIMER_LEFT_1_SEQUENCE=ATGACTGAGCAGATGACCCT
PRIMER_RIGHT_1_SEQUENCE=TCTAGCGTGTGCCAATGGT
PRIMER_LEFT_1=95,20
PRIMER_RIGHT_1=1049,19
PRIMER_LEFT_1_TM=57.211
PRIMER_RIGHT_1_TM=60.279
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=1.500000
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=955
PRIMER_PAIR_1_PRODUCT_TM=81.7
PRIMER_PAIR_2_PENALTY=6.289329
PRIMER_LEFT_2_PENALTY=2.658657
PRIMER_RIGHT_2_PENALTY=3.630671
PRIMER_LEFT_2_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_2_SEQUENCE=TTCTAGCGTGTGCCAATGGT
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=1050,20
PRIMER_LEFT_2_TM=60.659
PRIMER_RIGHT_2_TM=61.631
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=2.000000
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=956
PRIMER_PAIR_2_PRODUCT_TM=81.7
PRIMER_PAIR_3_PENALTY=6.289706
PRIMER_LEFT_3_PENALTY=2.241606
PRIMER_RIGHT_3_PENALTY=4.048100
PRIMER_LEFT_3_SEQUENCE=ATGACTGAGCAGATGACCCTT
PRIMER_RIGHT_3_SEQUENCE=ACTTCTAGCGTGTGCCAATG
PRIMER_LEFT_3=95,21
PRIMER_RIGHT_3=1052,20
PRIMER_LEFT_3_TM=58.758
PRIMER_RIGHT_3_TM=58.952
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=3.000000
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=958
PRIMER_PAIR_3_PRODUCT_TM=81.7
PRIMER_PAIR_4_PENALTY=6.419457
PRIMER_LEFT_4_PENALTY=2.788786
PRIMER_RIGHT_4_PENALTY=3.630671
PRIMER_LEFT_4_SEQUENCE=ATGACTGAGCAGATGACCCT
PRIMER_RIGHT_4_SEQUENCE=TTCTAGCGTGTGCCAATGGT
PRIMER_LEFT_4=95,20
PRIMER_RIGHT_4=1050,20
PRIMER_LEFT_4_TM=57.211
PRIMER_RIGHT_4_TM=61.631
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=2.000000
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=956
PRIMER_PAIR_4_PRODUCT_TM=81.7
PRIMER_PAIR_5_PENALTY=6.706757
PRIMER_LEFT_5_PENALTY=2.658657
PRIMER_RIGHT_5_PENALTY=4.048100
PRIMER_LEFT_5_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_5_SEQUENCE=ACTTCTAGCGTGTGCCAATG
PRIMER_LEFT_5=95,22
PRIMER_RIGHT_5=1052,20
PRIMER_LEFT_5_TM=60.659
PRIMER_RIGHT_5_TM=58.952
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=3.000000
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=958
PRIMER_PAIR_5_PRODUCT_TM=81.7
PRIMER_PAIR_6_PENALTY=6.836886
PRIMER_LEFT_6_PENALTY=2.788786
PRIMER_RIGHT_6_PENALTY=4.048100
PRIMER_LEFT_6_SEQUENCE=ATGACTGAGCAGATGACCCT
PRIMER_RIGHT_6_SEQUENCE=ACTTCTAGCGTGTGCCAATG
PRIMER_LEFT_6=95,20
PRIMER_RIGHT_6=1052,20
PRIMER_LEFT_6_TM=57.211
PRIMER_RIGHT_6_TM=58.952
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
PRIMER_LEFT_6_END_STABILITY=9.1000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=958
PRIMER_PAIR_6_PRODUCT_TM=81.7
PRIMER_PAIR_7_PENALTY=7.061025
PRIMER_LEFT_7_PENALTY=2.241606
PRIMER_RIGHT_7_PENALTY=4.819419
PRIMER_LEFT_7_SEQUENCE=ATGACTGAGCAGATGACCCTT
PRIMER_RIGHT_7_SEQUENCE=AACTTCTAGCGTGTGCCAATG
PRIMER_LEFT_7=95,21
PRIMER_RIGHT_7=1053,21
PRIMER_LEFT_7_TM=58.758
PRIMER_RIGHT_7_TM=60.319
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=3.500000
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=959
PRIMER_PAIR_7_PRODUCT_TM=81.6
PRIMER_PAIR_8_PENALTY=7.478076
PRIMER_LEFT_8_PENALTY=2.658657
PRIMER_RIGHT_8_PENALTY=4.819419
PRIMER_LEFT_8_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_8_SEQUENCE=AACTTCTAGCGTGTGCCAATG
PRIMER_LEFT_8=95,22
PRIMER_RIGHT_8=1053,21
PRIMER_LEFT_8_TM=60.659
PRIMER_RIGHT_8_TM=60.319
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=959
PRIMER_PAIR_8_PRODUCT_TM=81.6
PRIMER_PAIR_9_PENALTY=7.608205
PRIMER_LEFT_9_PENALTY=2.788786
PRIMER_RIGHT_9_PENALTY=4.819419
PRIMER_LEFT_9_SEQUENCE=ATGACTGAGCAGATGACCCT
PRIMER_RIGHT_9_SEQUENCE=AACTTCTAGCGTGTGCCAATG
PRIMER_LEFT_9=95,20
PRIMER_RIGHT_9=1053,21
PRIMER_LEFT_9_TM=57.211
PRIMER_RIGHT_9_TM=60.319
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=3.500000
PRIMER_LEFT_9_END_STABILITY=9.1000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=959
PRIMER_PAIR_9_PRODUCT_TM=81.6
PRIMER_PAIR_10_PENALTY=7.661525
PRIMER_LEFT_10_PENALTY=2.241606
PRIMER_RIGHT_10_PENALTY=5.419920
PRIMER_LEFT_10_SEQUENCE=ATGACTGAGCAGATGACCCTT
PRIMER_RIGHT_10_SEQUENCE=AAACTTCTAGCGTGTGCCAA
PRIMER_LEFT_10=95,21
PRIMER_RIGHT_10=1054,20
PRIMER_LEFT_10_TM=58.758
PRIMER_RIGHT_10_TM=58.580
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=4.000000
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=10.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=960
PRIMER_PAIR_10_PRODUCT_TM=81.6
PRIMER_PAIR_11_PENALTY=7.728937
PRIMER_LEFT_11_PENALTY=2.241606
PRIMER_RIGHT_11_PENALTY=5.487331
PRIMER_LEFT_11_SEQUENCE=ATGACTGAGCAGATGACCCTT
PRIMER_RIGHT_11_SEQUENCE=CTTCTAGCGTGTGCCAATG
PRIMER_LEFT_11=95,21
PRIMER_RIGHT_11=1051,19
PRIMER_LEFT_11_TM=58.758
PRIMER_RIGHT_11_TM=58.013
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=52.632
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=2.500000
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=957
PRIMER_PAIR_11_PRODUCT_TM=81.7
PRIMER_PAIR_12_PENALTY=8.078577
PRIMER_LEFT_12_PENALTY=2.658657
PRIMER_RIGHT_12_PENALTY=5.419920
PRIMER_LEFT_12_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_12_SEQUENCE=AAACTTCTAGCGTGTGCCAA
PRIMER_LEFT_12=95,22
PRIMER_RIGHT_12=1054,20
PRIMER_LEFT_12_TM=60.659
PRIMER_RIGHT_12_TM=58.580
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=4.000000
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=10.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=960
PRIMER_PAIR_12_PRODUCT_TM=81.6
PRIMER_PAIR_13_PENALTY=8.145989
PRIMER_LEFT_13_PENALTY=2.658657
PRIMER_RIGHT_13_PENALTY=5.487331
PRIMER_LEFT_13_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_13_SEQUENCE=CTTCTAGCGTGTGCCAATG
PRIMER_LEFT_13=95,22
PRIMER_RIGHT_13=1051,19
PRIMER_LEFT_13_TM=60.659
PRIMER_RIGHT_13_TM=58.013
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=2.500000
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=957
PRIMER_PAIR_13_PRODUCT_TM=81.7
PRIMER_PAIR_14_PENALTY=8.208706
PRIMER_LEFT_14_PENALTY=2.788786
PRIMER_RIGHT_14_PENALTY=5.419920
PRIMER_LEFT_14_SEQUENCE=ATGACTGAGCAGATGACCCT
PRIMER_RIGHT_14_SEQUENCE=AAACTTCTAGCGTGTGCCAA
PRIMER_LEFT_14=95,20
PRIMER_RIGHT_14=1054,20
PRIMER_LEFT_14_TM=57.211
PRIMER_RIGHT_14_TM=58.580
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=4.000000
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=10.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=960
PRIMER_PAIR_14_PRODUCT_TM=81.6
PRIMER_PAIR_15_PENALTY=8.232043
PRIMER_LEFT_15_PENALTY=2.241606
PRIMER_RIGHT_15_PENALTY=5.990437
PRIMER_LEFT_15_SEQUENCE=ATGACTGAGCAGATGACCCTT
PRIMER_RIGHT_15_SEQUENCE=AACTTCTAGCGTGTGCCAAT
PRIMER_LEFT_15=95,21
PRIMER_RIGHT_15=1053,20
PRIMER_LEFT_15_TM=58.758
PRIMER_RIGHT_15_TM=57.510
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=3.500000
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=959
PRIMER_PAIR_15_PRODUCT_TM=81.6
PRIMER_PAIR_16_PENALTY=8.276117
PRIMER_LEFT_16_PENALTY=2.788786
PRIMER_RIGHT_16_PENALTY=5.487331
PRIMER_LEFT_16_SEQUENCE=ATGACTGAGCAGATGACCCT
PRIMER_RIGHT_16_SEQUENCE=CTTCTAGCGTGTGCCAATG
PRIMER_LEFT_16=95,20
PRIMER_RIGHT_16=1051,19
PRIMER_LEFT_16_TM=57.211
PRIMER_RIGHT_16_TM=58.013
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.632
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=2.500000
PRIMER_LEFT_16_END_STABILITY=9.1000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=957
PRIMER_PAIR_16_PRODUCT_TM=81.7
PRIMER_PAIR_17_PENALTY=8.322092
PRIMER_LEFT_17_PENALTY=2.241606
PRIMER_RIGHT_17_PENALTY=6.080486
PRIMER_LEFT_17_SEQUENCE=ATGACTGAGCAGATGACCCTT
PRIMER_RIGHT_17_SEQUENCE=AAACTTCTAGCGTGTGCCAAT
PRIMER_LEFT_17=95,21
PRIMER_RIGHT_17=1054,21
PRIMER_LEFT_17_TM=58.758
PRIMER_RIGHT_17_TM=58.920
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=4.000000
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=960
PRIMER_PAIR_17_PRODUCT_TM=81.6
PRIMER_PAIR_18_PENALTY=8.649095
PRIMER_LEFT_18_PENALTY=2.658657
PRIMER_RIGHT_18_PENALTY=5.990437
PRIMER_LEFT_18_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_18_SEQUENCE=AACTTCTAGCGTGTGCCAAT
PRIMER_LEFT_18=95,22
PRIMER_RIGHT_18=1053,20
PRIMER_LEFT_18_TM=60.659
PRIMER_RIGHT_18_TM=57.510
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=3.500000
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=959
PRIMER_PAIR_18_PRODUCT_TM=81.6
PRIMER_PAIR_19_PENALTY=8.650935
PRIMER_LEFT_19_PENALTY=2.658657
PRIMER_RIGHT_19_PENALTY=5.992277
PRIMER_LEFT_19_SEQUENCE=ATGACTGAGCAGATGACCCTTC
PRIMER_RIGHT_19_SEQUENCE=CTTCTAGCGTGTGCCAATGGT
PRIMER_LEFT_19=95,22
PRIMER_RIGHT_19=1051,21
PRIMER_LEFT_19_TM=60.659
PRIMER_RIGHT_19_TM=62.492
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=2.500000
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.8000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=957
PRIMER_PAIR_19_PRODUCT_TM=81.7
=
SEQUENCE_ID=X66357/CDK3
P3_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS_89..1006
SEQUENCE_START_CODON_POSITION=88
SEQUENCE_TEMPLATE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC
PRIMER_LEFT_EXPLAIN=considered 9505, would not amplify any of the ORF 6576, GC content failed 22, low tm 213, high tm 1911, high 3' stability 39, ok 744
PRIMER_RIGHT_EXPLAIN=considered 9326, GC content failed 54, low tm 682, high tm 6041, high any compl 1, high end compl 3, high 3' stability 58, ok 2487
PRIMER_PAIR_EXPLAIN=considered 22, ok 22
PRIMER_STOP_CODON_POSITION=1003
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=9.663260
PRIMER_LEFT_0_PENALTY=5.563683
PRIMER_RIGHT_0_PENALTY=4.099578
PRIMER_LEFT_0_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_0_SEQUENCE=ACATTCTCAATGGCGGAATC
PRIMER_LEFT_0=88,25
PRIMER_RIGHT_0=1011,20
PRIMER_LEFT_0_TM=59.436
PRIMER_RIGHT_0_TM=59.900
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=4.000000
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=924
PRIMER_PAIR_0_PRODUCT_TM=82.8
PRIMER_PAIR_1_PENALTY=10.331136
PRIMER_LEFT_1_PENALTY=6.231558
PRIMER_RIGHT_1_PENALTY=4.099578
PRIMER_LEFT_1_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
PRIMER_RIGHT_1_SEQUENCE=ACATTCTCAATGGCGGAATC
PRIMER_LEFT_1=88,24
PRIMER_RIGHT_1=1011,20
PRIMER_LEFT_1_TM=57.768
PRIMER_RIGHT_1_TM=59.900
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=4.000000
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=924
PRIMER_PAIR_1_PRODUCT_TM=82.8
PRIMER_PAIR_2_PENALTY=10.521269
PRIMER_LEFT_2_PENALTY=5.563683
PRIMER_RIGHT_2_PENALTY=4.957587
PRIMER_LEFT_2_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_2_SEQUENCE=ATTCTCAATGGCGGAATCG
PRIMER_LEFT_2=88,25
PRIMER_RIGHT_2=1009,19
PRIMER_LEFT_2_TM=59.436
PRIMER_RIGHT_2_TM=60.958
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=47.368
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=3.000000
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=922
PRIMER_PAIR_2_PRODUCT_TM=82.8
PRIMER_PAIR_3_PENALTY=10.670942
PRIMER_LEFT_3_PENALTY=6.571364
PRIMER_RIGHT_3_PENALTY=4.099578
PRIMER_LEFT_3_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
PRIMER_RIGHT_3_SEQUENCE=ACATTCTCAATGGCGGAATC
PRIMER_LEFT_3=88,26
PRIMER_RIGHT_3=1011,20
PRIMER_LEFT_3_TM=60.571
PRIMER_RIGHT_3_TM=59.900
PRIMER_LEFT_3_GC_PERCENT=38.462
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=4.000000
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=924
PRIMER_PAIR_3_PRODUCT_TM=82.8
PRIMER_PAIR_4_PENALTY=11.048511
PRIMER_LEFT_4_PENALTY=5.563683
PRIMER_RIGHT_4_PENALTY=5.484828
PRIMER_LEFT_4_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_4_SEQUENCE=CATTCTCAATGGCGGAATC
PRIMER_LEFT_4=88,25
PRIMER_RIGHT_4=1010,19
PRIMER_LEFT_4_TM=59.436
PRIMER_RIGHT_4_TM=59.015
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=47.368
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=3.500000
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=923
PRIMER_PAIR_4_PRODUCT_TM=82.8
PRIMER_PAIR_5_PENALTY=11.189145
PRIMER_LEFT_5_PENALTY=6.231558
PRIMER_RIGHT_5_PENALTY=4.957587
PRIMER_LEFT_5_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
PRIMER_RIGHT_5_SEQUENCE=ATTCTCAATGGCGGAATCG
PRIMER_LEFT_5=88,24
PRIMER_RIGHT_5=1009,19
PRIMER_LEFT_5_TM=57.768
PRIMER_RIGHT_5_TM=60.958
PRIMER_LEFT_5_GC_PERCENT=41.667
PRIMER_RIGHT_5_GC_PERCENT=47.368
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=3.000000
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=8.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=922
PRIMER_PAIR_5_PRODUCT_TM=82.8
PRIMER_PAIR_6_PENALTY=11.528951
PRIMER_LEFT_6_PENALTY=6.571364
PRIMER_RIGHT_6_PENALTY=4.957587
PRIMER_LEFT_6_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
PRIMER_RIGHT_6_SEQUENCE=ATTCTCAATGGCGGAATCG
PRIMER_LEFT_6=88,26
PRIMER_RIGHT_6=1009,19
PRIMER_LEFT_6_TM=60.571
PRIMER_RIGHT_6_TM=60.958
PRIMER_LEFT_6_GC_PERCENT=38.462
PRIMER_RIGHT_6_GC_PERCENT=47.368
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=922
PRIMER_PAIR_6_PRODUCT_TM=82.8
PRIMER_PAIR_7_PENALTY=11.716386
PRIMER_LEFT_7_PENALTY=6.231558
PRIMER_RIGHT_7_PENALTY=5.484828
PRIMER_LEFT_7_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
PRIMER_RIGHT_7_SEQUENCE=CATTCTCAATGGCGGAATC
PRIMER_LEFT_7=88,24
PRIMER_RIGHT_7=1010,19
PRIMER_LEFT_7_TM=57.768
PRIMER_RIGHT_7_TM=59.015
PRIMER_LEFT_7_GC_PERCENT=41.667
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=3.500000
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=923
PRIMER_PAIR_7_PRODUCT_TM=82.8
PRIMER_PAIR_8_PENALTY=12.056192
PRIMER_LEFT_8_PENALTY=6.571364
PRIMER_RIGHT_8_PENALTY=5.484828
PRIMER_LEFT_8_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
PRIMER_RIGHT_8_SEQUENCE=CATTCTCAATGGCGGAATC
PRIMER_LEFT_8=88,26
PRIMER_RIGHT_8=1010,19
PRIMER_LEFT_8_TM=60.571
PRIMER_RIGHT_8_TM=59.015
PRIMER_LEFT_8_GC_PERCENT=38.462
PRIMER_RIGHT_8_GC_PERCENT=47.368
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=923
PRIMER_PAIR_8_PRODUCT_TM=82.8
PRIMER_PAIR_9_PENALTY=12.416538
PRIMER_LEFT_9_PENALTY=8.316960
PRIMER_RIGHT_9_PENALTY=4.099578
PRIMER_LEFT_9_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG
PRIMER_RIGHT_9_SEQUENCE=ACATTCTCAATGGCGGAATC
PRIMER_LEFT_9=88,27
PRIMER_RIGHT_9=1011,20
PRIMER_LEFT_9_TM=61.317
PRIMER_RIGHT_9_TM=59.900
PRIMER_LEFT_9_GC_PERCENT=40.741
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=4.000000
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=924
PRIMER_PAIR_9_PRODUCT_TM=82.8
PRIMER_PAIR_10_PENALTY=12.669516
PRIMER_LEFT_10_PENALTY=5.563683
PRIMER_RIGHT_10_PENALTY=7.105834
PRIMER_LEFT_10_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_10_SEQUENCE=TCTCAATGGCGGAATCGCT
PRIMER_LEFT_10=88,25
PRIMER_RIGHT_10=1007,19
PRIMER_LEFT_10_TM=59.436
PRIMER_RIGHT_10_TM=64.106
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=52.632
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=9.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=920
PRIMER_PAIR_10_PRODUCT_TM=82.8
PRIMER_PAIR_11_PENALTY=12.776612
PRIMER_LEFT_11_PENALTY=5.563683
PRIMER_RIGHT_11_PENALTY=7.212929
PRIMER_LEFT_11_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_11_SEQUENCE=CATTCTCAATGGCGGAATCG
PRIMER_LEFT_11=88,25
PRIMER_RIGHT_11=1010,20
PRIMER_LEFT_11_TM=59.436
PRIMER_RIGHT_11_TM=63.713
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=3.500000
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=8.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=923
PRIMER_PAIR_11_PRODUCT_TM=82.8
PRIMER_PAIR_12_PENALTY=12.799060
PRIMER_LEFT_12_PENALTY=5.563683
PRIMER_RIGHT_12_PENALTY=7.235377
PRIMER_LEFT_12_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_12_SEQUENCE=GACATTCTCAATGGCGGAATC
PRIMER_LEFT_12=88,25
PRIMER_RIGHT_12=1012,21
PRIMER_LEFT_12_TM=59.436
PRIMER_RIGHT_12_TM=61.735
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=4.500000
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=925
PRIMER_PAIR_12_PRODUCT_TM=82.8
PRIMER_PAIR_13_PENALTY=13.055628
PRIMER_LEFT_13_PENALTY=5.563683
PRIMER_RIGHT_13_PENALTY=7.491945
PRIMER_LEFT_13_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_13_SEQUENCE=ATTCTCAATGGCGGAATCGC
PRIMER_LEFT_13=88,25
PRIMER_RIGHT_13=1009,20
PRIMER_LEFT_13_TM=59.436
PRIMER_RIGHT_13_TM=64.492
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=3.000000
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=9.8000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=922
PRIMER_PAIR_13_PRODUCT_TM=82.8
PRIMER_PAIR_14_PENALTY=13.274547
PRIMER_LEFT_14_PENALTY=8.316960
PRIMER_RIGHT_14_PENALTY=4.957587
PRIMER_LEFT_14_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG
PRIMER_RIGHT_14_SEQUENCE=ATTCTCAATGGCGGAATCG
PRIMER_LEFT_14=88,27
PRIMER_RIGHT_14=1009,19
PRIMER_LEFT_14_TM=61.317
PRIMER_RIGHT_14_TM=60.958
PRIMER_LEFT_14_GC_PERCENT=40.741
PRIMER_RIGHT_14_GC_PERCENT=47.368
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=3.000000
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=922
PRIMER_PAIR_14_PRODUCT_TM=82.8
PRIMER_PAIR_15_PENALTY=13.337392
PRIMER_LEFT_15_PENALTY=6.231558
PRIMER_RIGHT_15_PENALTY=7.105834
PRIMER_LEFT_15_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
PRIMER_RIGHT_15_SEQUENCE=TCTCAATGGCGGAATCGCT
PRIMER_LEFT_15=88,24
PRIMER_RIGHT_15=1007,19
PRIMER_LEFT_15_TM=57.768
PRIMER_RIGHT_15_TM=64.106
PRIMER_LEFT_15_GC_PERCENT=41.667
PRIMER_RIGHT_15_GC_PERCENT=52.632
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=2.000000
PRIMER_LEFT_15_END_STABILITY=5.7000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=920
PRIMER_PAIR_15_PRODUCT_TM=82.8
PRIMER_PAIR_16_PENALTY=13.444487
PRIMER_LEFT_16_PENALTY=6.231558
PRIMER_RIGHT_16_PENALTY=7.212929
PRIMER_LEFT_16_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
PRIMER_RIGHT_16_SEQUENCE=CATTCTCAATGGCGGAATCG
PRIMER_LEFT_16=88,24
PRIMER_RIGHT_16=1010,20
PRIMER_LEFT_16_TM=57.768
PRIMER_RIGHT_16_TM=63.713
PRIMER_LEFT_16_GC_PERCENT=41.667
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=8.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=923
PRIMER_PAIR_16_PRODUCT_TM=82.8
PRIMER_PAIR_17_PENALTY=13.466935
PRIMER_LEFT_17_PENALTY=6.231558
PRIMER_RIGHT_17_PENALTY=7.235377
PRIMER_LEFT_17_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
PRIMER_RIGHT_17_SEQUENCE=GACATTCTCAATGGCGGAATC
PRIMER_LEFT_17=88,24
PRIMER_RIGHT_17=1012,21
PRIMER_LEFT_17_TM=57.768
PRIMER_RIGHT_17_TM=61.735
PRIMER_LEFT_17_GC_PERCENT=41.667
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=4.500000
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=925
PRIMER_PAIR_17_PRODUCT_TM=82.8
PRIMER_PAIR_18_PENALTY=13.522601
PRIMER_LEFT_18_PENALTY=5.563683
PRIMER_RIGHT_18_PENALTY=7.958919
PRIMER_LEFT_18_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
PRIMER_RIGHT_18_SEQUENCE=TTCTCAATGGCGGAATCGC
PRIMER_LEFT_18=88,25
PRIMER_RIGHT_18=1008,19
PRIMER_LEFT_18_TM=59.436
PRIMER_RIGHT_18_TM=64.459
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=52.632
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=2.500000
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=9.8000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=921
PRIMER_PAIR_18_PRODUCT_TM=82.8
PRIMER_PAIR_19_PENALTY=13.677198
PRIMER_LEFT_19_PENALTY=6.571364
PRIMER_RIGHT_19_PENALTY=7.105834
PRIMER_LEFT_19_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
PRIMER_RIGHT_19_SEQUENCE=TCTCAATGGCGGAATCGCT
PRIMER_LEFT_19=88,26
PRIMER_RIGHT_19=1007,19
PRIMER_LEFT_19_TM=60.571
PRIMER_RIGHT_19_TM=64.106
PRIMER_LEFT_19_GC_PERCENT=38.462
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=2.000000
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=920
PRIMER_PAIR_19_PRODUCT_TM=82.8
=
SEQUENCE_ID=X66364/CDK5
P3_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS_25..903
SEQUENCE_START_CODON_POSITION=24
SEQUENCE_TEMPLATE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG
PRIMER_LEFT_EXPLAIN=considered 7904, would not amplify any of the ORF 5278, GC content failed 48, low tm 249, high tm 1503, high 3' stability 40, ok 786
PRIMER_RIGHT_EXPLAIN=considered 7709, GC content failed 194, low tm 667, high tm 4515, high 3' stability 60, ok 2273
PRIMER_PAIR_EXPLAIN=considered 21, ok 21
PRIMER_STOP_CODON_POSITION=900
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=5.830871
PRIMER_LEFT_0_PENALTY=3.142611
PRIMER_RIGHT_0_PENALTY=2.688260
PRIMER_LEFT_0_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_0_SEQUENCE=CCTAGGGCGGACAGAAGTC
PRIMER_LEFT_0=24,23
PRIMER_RIGHT_0=903,19
PRIMER_LEFT_0_TM=60.143
PRIMER_RIGHT_0_TM=59.812
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=1.500000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=880
PRIMER_PAIR_0_PRODUCT_TM=81.4
PRIMER_PAIR_1_PENALTY=6.081075
PRIMER_LEFT_1_PENALTY=3.142611
PRIMER_RIGHT_1_PENALTY=2.938464
PRIMER_LEFT_1_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_1_SEQUENCE=CTAGGGCGGACAGAAGTCG
PRIMER_LEFT_1=24,23
PRIMER_RIGHT_1=902,19
PRIMER_LEFT_1_TM=60.143
PRIMER_RIGHT_1_TM=60.938
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=63.158
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=1.000000
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=879
PRIMER_PAIR_1_PRODUCT_TM=81.4
PRIMER_PAIR_2_PENALTY=6.285566
PRIMER_LEFT_2_PENALTY=3.597306
PRIMER_RIGHT_2_PENALTY=2.688260
PRIMER_LEFT_2_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_2_SEQUENCE=CCTAGGGCGGACAGAAGTC
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=903,19
PRIMER_LEFT_2_TM=58.403
PRIMER_RIGHT_2_TM=59.812
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=63.158
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=1.500000
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=880
PRIMER_PAIR_2_PRODUCT_TM=81.4
PRIMER_PAIR_3_PENALTY=6.535770
PRIMER_LEFT_3_PENALTY=3.597306
PRIMER_RIGHT_3_PENALTY=2.938464
PRIMER_LEFT_3_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_3_SEQUENCE=CTAGGGCGGACAGAAGTCG
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=902,19
PRIMER_LEFT_3_TM=58.403
PRIMER_RIGHT_3_TM=60.938
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=879
PRIMER_PAIR_3_PRODUCT_TM=81.4
PRIMER_PAIR_4_PENALTY=7.847014
PRIMER_LEFT_4_PENALTY=3.142611
PRIMER_RIGHT_4_PENALTY=4.704404
PRIMER_LEFT_4_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_4_SEQUENCE=GCCTAGGGCGGACAGAAGT
PRIMER_LEFT_4=24,23
PRIMER_RIGHT_4=904,19
PRIMER_LEFT_4_TM=60.143
PRIMER_RIGHT_4_TM=61.704
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=2.000000
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=881
PRIMER_PAIR_4_PRODUCT_TM=81.5
PRIMER_PAIR_5_PENALTY=7.990463
PRIMER_LEFT_5_PENALTY=5.302203
PRIMER_RIGHT_5_PENALTY=2.688260
PRIMER_LEFT_5_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
PRIMER_RIGHT_5_SEQUENCE=CCTAGGGCGGACAGAAGTC
PRIMER_LEFT_5=24,24
PRIMER_RIGHT_5=903,19
PRIMER_LEFT_5_TM=61.302
PRIMER_RIGHT_5_TM=59.812
PRIMER_LEFT_5_GC_PERCENT=37.500
PRIMER_RIGHT_5_GC_PERCENT=63.158
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=1.500000
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=880
PRIMER_PAIR_5_PRODUCT_TM=81.4
PRIMER_PAIR_6_PENALTY=8.240667
PRIMER_LEFT_6_PENALTY=5.302203
PRIMER_RIGHT_6_PENALTY=2.938464
PRIMER_LEFT_6_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
PRIMER_RIGHT_6_SEQUENCE=CTAGGGCGGACAGAAGTCG
PRIMER_LEFT_6=24,24
PRIMER_RIGHT_6=902,19
PRIMER_LEFT_6_TM=61.302
PRIMER_RIGHT_6_TM=60.938
PRIMER_LEFT_6_GC_PERCENT=37.500
PRIMER_RIGHT_6_GC_PERCENT=63.158
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=879
PRIMER_PAIR_6_PRODUCT_TM=81.4
PRIMER_PAIR_7_PENALTY=8.244153
PRIMER_LEFT_7_PENALTY=3.142611
PRIMER_RIGHT_7_PENALTY=5.101542
PRIMER_LEFT_7_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_7_SEQUENCE=TAGGGCGGACAGAAGTCGG
PRIMER_LEFT_7=24,23
PRIMER_RIGHT_7=901,19
PRIMER_LEFT_7_TM=60.143
PRIMER_RIGHT_7_TM=63.602
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=63.158
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=0.500000
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=878
PRIMER_PAIR_7_PRODUCT_TM=81.4
PRIMER_PAIR_8_PENALTY=8.301709
PRIMER_LEFT_8_PENALTY=3.597306
PRIMER_RIGHT_8_PENALTY=4.704404
PRIMER_LEFT_8_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_8_SEQUENCE=GCCTAGGGCGGACAGAAGT
PRIMER_LEFT_8=24,22
PRIMER_RIGHT_8=904,19
PRIMER_LEFT_8_TM=58.403
PRIMER_RIGHT_8_TM=61.704
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=63.158
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=2.000000
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=881
PRIMER_PAIR_8_PRODUCT_TM=81.5
PRIMER_PAIR_9_PENALTY=8.517669
PRIMER_LEFT_9_PENALTY=3.142611
PRIMER_RIGHT_9_PENALTY=5.375058
PRIMER_LEFT_9_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_9_SEQUENCE=CTAGGGCGGACAGAAGTCGG
PRIMER_LEFT_9=24,23
PRIMER_RIGHT_9=902,20
PRIMER_LEFT_9_TM=60.143
PRIMER_RIGHT_9_TM=64.375
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=65.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=1.000000
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=879
PRIMER_PAIR_9_PRODUCT_TM=81.4
PRIMER_PAIR_10_PENALTY=8.611012
PRIMER_LEFT_10_PENALTY=3.142611
PRIMER_RIGHT_10_PENALTY=5.468402
PRIMER_LEFT_10_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_10_SEQUENCE=GCCTAGGGCGGACAGAAGTC
PRIMER_LEFT_10=24,23
PRIMER_RIGHT_10=904,20
PRIMER_LEFT_10_TM=60.143
PRIMER_RIGHT_10_TM=63.468
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=65.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=881
PRIMER_PAIR_10_PRODUCT_TM=81.5
PRIMER_PAIR_11_PENALTY=8.698847
PRIMER_LEFT_11_PENALTY=3.597306
PRIMER_RIGHT_11_PENALTY=5.101542
PRIMER_LEFT_11_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_11_SEQUENCE=TAGGGCGGACAGAAGTCGG
PRIMER_LEFT_11=24,22
PRIMER_RIGHT_11=901,19
PRIMER_LEFT_11_TM=58.403
PRIMER_RIGHT_11_TM=63.602
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=63.158
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=0.500000
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=878
PRIMER_PAIR_11_PRODUCT_TM=81.4
PRIMER_PAIR_12_PENALTY=8.972363
PRIMER_LEFT_12_PENALTY=3.597306
PRIMER_RIGHT_12_PENALTY=5.375058
PRIMER_LEFT_12_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_12_SEQUENCE=CTAGGGCGGACAGAAGTCGG
PRIMER_LEFT_12=24,22
PRIMER_RIGHT_12=902,20
PRIMER_LEFT_12_TM=58.403
PRIMER_RIGHT_12_TM=64.375
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=65.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=1.000000
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=879
PRIMER_PAIR_12_PRODUCT_TM=81.4
PRIMER_PAIR_13_PENALTY=9.017669
PRIMER_LEFT_13_PENALTY=3.142611
PRIMER_RIGHT_13_PENALTY=5.875058
PRIMER_LEFT_13_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
PRIMER_RIGHT_13_SEQUENCE=CCTAGGGCGGACAGAAGTCG
PRIMER_LEFT_13=24,23
PRIMER_RIGHT_13=903,20
PRIMER_LEFT_13_TM=60.143
PRIMER_RIGHT_13_TM=64.375
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=65.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=1.500000
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=880
PRIMER_PAIR_13_PRODUCT_TM=81.4
PRIMER_PAIR_14_PENALTY=9.065707
PRIMER_LEFT_14_PENALTY=3.597306
PRIMER_RIGHT_14_PENALTY=5.468402
PRIMER_LEFT_14_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_14_SEQUENCE=GCCTAGGGCGGACAGAAGTC
PRIMER_LEFT_14=24,22
PRIMER_RIGHT_14=904,20
PRIMER_LEFT_14_TM=58.403
PRIMER_RIGHT_14_TM=63.468
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=65.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=2.000000
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=881
PRIMER_PAIR_14_PRODUCT_TM=81.5
PRIMER_PAIR_15_PENALTY=9.472363
PRIMER_LEFT_15_PENALTY=3.597306
PRIMER_RIGHT_15_PENALTY=5.875058
PRIMER_LEFT_15_SEQUENCE=ATGCAGAAATACGAGAAACTGG
PRIMER_RIGHT_15_SEQUENCE=CCTAGGGCGGACAGAAGTCG
PRIMER_LEFT_15=24,22
PRIMER_RIGHT_15=903,20
PRIMER_LEFT_15_TM=58.403
PRIMER_RIGHT_15_TM=64.375
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=65.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=1.500000
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=880
PRIMER_PAIR_15_PRODUCT_TM=81.4
PRIMER_PAIR_16_PENALTY=10.006607
PRIMER_LEFT_16_PENALTY=5.302203
PRIMER_RIGHT_16_PENALTY=4.704404
PRIMER_LEFT_16_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
PRIMER_RIGHT_16_SEQUENCE=GCCTAGGGCGGACAGAAGT
PRIMER_LEFT_16=24,24
PRIMER_RIGHT_16=904,19
PRIMER_LEFT_16_TM=61.302
PRIMER_RIGHT_16_TM=61.704
PRIMER_LEFT_16_GC_PERCENT=37.500
PRIMER_RIGHT_16_GC_PERCENT=63.158
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=2.000000
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=881
PRIMER_PAIR_16_PRODUCT_TM=81.5
PRIMER_PAIR_17_PENALTY=10.044329
PRIMER_LEFT_17_PENALTY=7.356069
PRIMER_RIGHT_17_PENALTY=2.688260
PRIMER_LEFT_17_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA
PRIMER_RIGHT_17_SEQUENCE=CCTAGGGCGGACAGAAGTC
PRIMER_LEFT_17=24,25
PRIMER_RIGHT_17=903,19
PRIMER_LEFT_17_TM=62.356
PRIMER_RIGHT_17_TM=59.812
PRIMER_LEFT_17_GC_PERCENT=36.000
PRIMER_RIGHT_17_GC_PERCENT=63.158
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=880
PRIMER_PAIR_17_PRODUCT_TM=81.4
PRIMER_PAIR_18_PENALTY=10.294533
PRIMER_LEFT_18_PENALTY=7.356069
PRIMER_RIGHT_18_PENALTY=2.938464
PRIMER_LEFT_18_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA
PRIMER_RIGHT_18_SEQUENCE=CTAGGGCGGACAGAAGTCG
PRIMER_LEFT_18=24,25
PRIMER_RIGHT_18=902,19
PRIMER_LEFT_18_TM=62.356
PRIMER_RIGHT_18_TM=60.938
PRIMER_LEFT_18_GC_PERCENT=36.000
PRIMER_RIGHT_18_GC_PERCENT=63.158
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=1.000000
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=879
PRIMER_PAIR_18_PRODUCT_TM=81.4
PRIMER_PAIR_19_PENALTY=10.403745
PRIMER_LEFT_19_PENALTY=5.302203
PRIMER_RIGHT_19_PENALTY=5.101542
PRIMER_LEFT_19_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
PRIMER_RIGHT_19_SEQUENCE=TAGGGCGGACAGAAGTCGG
PRIMER_LEFT_19=24,24
PRIMER_RIGHT_19=901,19
PRIMER_LEFT_19_TM=61.302
PRIMER_RIGHT_19_TM=63.602
PRIMER_LEFT_19_GC_PERCENT=37.500
PRIMER_RIGHT_19_GC_PERCENT=63.158
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=0.500000
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=878
PRIMER_PAIR_19_PRODUCT_TM=81.4
=
SEQUENCE_ID=L34673/HIP116
P3_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS_178..3207
SEQUENCE_START_CODON_POSITION=177
SEQUENCE_TEMPLATE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT
PRIMER_LEFT_EXPLAIN=considered 29448, would not amplify any of the ORF 20059, GC content failed 202, low tm 3593, high tm 1850, long poly-x seq 41, high 3' stability 65, ok 3638
PRIMER_RIGHT_EXPLAIN=considered 29262, GC content failed 608, low tm 11948, high tm 5429, high end compl 9, long poly-x seq 56, high 3' stability 79, ok 11133
PRIMER_PAIR_EXPLAIN=considered 21, ok 21
PRIMER_STOP_CODON_POSITION=3204
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=15.950720
PRIMER_LEFT_0_PENALTY=1.996678
PRIMER_RIGHT_0_PENALTY=13.954041
PRIMER_LEFT_0_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_0_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
PRIMER_LEFT_0=177,21
PRIMER_RIGHT_0=3214,27
PRIMER_LEFT_0_TM=59.003
PRIMER_RIGHT_0_TM=58.046
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=25.926
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=5.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=3038
PRIMER_PAIR_0_PRODUCT_TM=75.4
PRIMER_PAIR_1_PENALTY=18.477471
PRIMER_LEFT_1_PENALTY=4.523430
PRIMER_RIGHT_1_PENALTY=13.954041
PRIMER_LEFT_1_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
PRIMER_RIGHT_1_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=3214,27
PRIMER_LEFT_1_TM=62.523
PRIMER_RIGHT_1_TM=58.046
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=25.926
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=5.000000
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=3038
PRIMER_PAIR_1_PRODUCT_TM=75.4
PRIMER_PAIR_2_PENALTY=19.375672
PRIMER_LEFT_2_PENALTY=1.996678
PRIMER_RIGHT_2_PENALTY=17.378993
PRIMER_LEFT_2_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_2_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_2=177,21
PRIMER_RIGHT_2=3221,26
PRIMER_LEFT_2_TM=59.003
PRIMER_RIGHT_2_TM=57.121
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=26.923
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=8.500000
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=3045
PRIMER_PAIR_2_PRODUCT_TM=75.4
PRIMER_PAIR_3_PENALTY=20.047681
PRIMER_LEFT_3_PENALTY=1.996678
PRIMER_RIGHT_3_PENALTY=18.051002
PRIMER_LEFT_3_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_3_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
PRIMER_LEFT_3=177,21
PRIMER_RIGHT_3=3221,27
PRIMER_LEFT_3_TM=59.003
PRIMER_RIGHT_3_TM=57.449
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=25.926
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=8.500000
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=3045
PRIMER_PAIR_3_PRODUCT_TM=75.4
PRIMER_PAIR_4_PENALTY=20.156411
PRIMER_LEFT_4_PENALTY=1.996678
PRIMER_RIGHT_4_PENALTY=18.159733
PRIMER_LEFT_4_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_4_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_4=177,21
PRIMER_RIGHT_4=3222,27
PRIMER_LEFT_4_TM=59.003
PRIMER_RIGHT_4_TM=57.840
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=25.926
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=9.000000
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=3046
PRIMER_PAIR_4_PRODUCT_TM=75.4
PRIMER_PAIR_5_PENALTY=21.147183
PRIMER_LEFT_5_PENALTY=7.193142
PRIMER_RIGHT_5_PENALTY=13.954041
PRIMER_LEFT_5_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
PRIMER_RIGHT_5_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
PRIMER_LEFT_5=177,23
PRIMER_RIGHT_5=3214,27
PRIMER_LEFT_5_TM=64.193
PRIMER_RIGHT_5_TM=58.046
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=25.926
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=3038
PRIMER_PAIR_5_PRODUCT_TM=75.4
PRIMER_PAIR_6_PENALTY=21.902423
PRIMER_LEFT_6_PENALTY=4.523430
PRIMER_RIGHT_6_PENALTY=17.378993
PRIMER_LEFT_6_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
PRIMER_RIGHT_6_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_6=177,22
PRIMER_RIGHT_6=3221,26
PRIMER_LEFT_6_TM=62.523
PRIMER_RIGHT_6_TM=57.121
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=26.923
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
PRIMER_RIGHT_6_POSITION_PENALTY=8.500000
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=3045
PRIMER_PAIR_6_PRODUCT_TM=75.4
PRIMER_PAIR_7_PENALTY=22.190785
PRIMER_LEFT_7_PENALTY=8.236744
PRIMER_RIGHT_7_PENALTY=13.954041
PRIMER_LEFT_7_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
PRIMER_RIGHT_7_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
PRIMER_LEFT_7=177,24
PRIMER_RIGHT_7=3214,27
PRIMER_LEFT_7_TM=64.237
PRIMER_RIGHT_7_TM=58.046
PRIMER_LEFT_7_GC_PERCENT=45.833
PRIMER_RIGHT_7_GC_PERCENT=25.926
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
PRIMER_LEFT_7_END_STABILITY=9.3000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=3038
PRIMER_PAIR_7_PRODUCT_TM=75.4
PRIMER_PAIR_8_PENALTY=22.574432
PRIMER_LEFT_8_PENALTY=4.523430
PRIMER_RIGHT_8_PENALTY=18.051002
PRIMER_LEFT_8_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
PRIMER_RIGHT_8_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
PRIMER_LEFT_8=177,22
PRIMER_RIGHT_8=3221,27
PRIMER_LEFT_8_TM=62.523
PRIMER_RIGHT_8_TM=57.449
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=25.926
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
PRIMER_RIGHT_8_POSITION_PENALTY=8.500000
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=3045
PRIMER_PAIR_8_PRODUCT_TM=75.4
PRIMER_PAIR_9_PENALTY=22.683163
PRIMER_LEFT_9_PENALTY=4.523430
PRIMER_RIGHT_9_PENALTY=18.159733
PRIMER_LEFT_9_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
PRIMER_RIGHT_9_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_9=177,22
PRIMER_RIGHT_9=3222,27
PRIMER_LEFT_9_TM=62.523
PRIMER_RIGHT_9_TM=57.840
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=25.926
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
PRIMER_RIGHT_9_POSITION_PENALTY=9.000000
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=3046
PRIMER_PAIR_9_PRODUCT_TM=75.4
PRIMER_PAIR_10_PENALTY=24.254496
PRIMER_LEFT_10_PENALTY=1.996678
PRIMER_RIGHT_10_PENALTY=22.257817
PRIMER_LEFT_10_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_10_SEQUENCE=TCTTCTTACTAAAATCCCACAAATTAT
PRIMER_LEFT_10=177,21
PRIMER_RIGHT_10=3229,27
PRIMER_LEFT_10_TM=59.003
PRIMER_RIGHT_10_TM=57.242
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=25.926
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
PRIMER_RIGHT_10_POSITION_PENALTY=12.500000
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=5.8000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=3053
PRIMER_PAIR_10_PRODUCT_TM=75.4
PRIMER_PAIR_11_PENALTY=24.257918
PRIMER_LEFT_11_PENALTY=1.996678
PRIMER_RIGHT_11_PENALTY=22.261240
PRIMER_LEFT_11_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_11_SEQUENCE=AGTCTTCTTACTAAAATCCCACAAAT
PRIMER_LEFT_11=177,21
PRIMER_RIGHT_11=3231,26
PRIMER_LEFT_11_TM=59.003
PRIMER_RIGHT_11_TM=57.239
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=30.769
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
PRIMER_RIGHT_11_POSITION_PENALTY=13.500000
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=3055
PRIMER_PAIR_11_PRODUCT_TM=75.4
PRIMER_PAIR_12_PENALTY=24.363903
PRIMER_LEFT_12_PENALTY=1.996678
PRIMER_RIGHT_12_PENALTY=22.367225
PRIMER_LEFT_12_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_12_SEQUENCE=GTCTTCTTACTAAAATCCCACAAATTA
PRIMER_LEFT_12=177,21
PRIMER_RIGHT_12=3230,27
PRIMER_LEFT_12_TM=59.003
PRIMER_RIGHT_12_TM=57.633
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=29.630
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
PRIMER_RIGHT_12_POSITION_PENALTY=13.000000
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=3054
PRIMER_PAIR_12_PRODUCT_TM=75.4
PRIMER_PAIR_13_PENALTY=24.572135
PRIMER_LEFT_13_PENALTY=7.193142
PRIMER_RIGHT_13_PENALTY=17.378993
PRIMER_LEFT_13_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
PRIMER_RIGHT_13_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_13=177,23
PRIMER_RIGHT_13=3221,26
PRIMER_LEFT_13_TM=64.193
PRIMER_RIGHT_13_TM=57.121
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=26.923
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
PRIMER_RIGHT_13_POSITION_PENALTY=8.500000
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=3045
PRIMER_PAIR_13_PRODUCT_TM=75.4
PRIMER_PAIR_14_PENALTY=25.244145
PRIMER_LEFT_14_PENALTY=7.193142
PRIMER_RIGHT_14_PENALTY=18.051002
PRIMER_LEFT_14_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
PRIMER_RIGHT_14_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
PRIMER_LEFT_14=177,23
PRIMER_RIGHT_14=3221,27
PRIMER_LEFT_14_TM=64.193
PRIMER_RIGHT_14_TM=57.449
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=25.926
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
PRIMER_RIGHT_14_POSITION_PENALTY=8.500000
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=3045
PRIMER_PAIR_14_PRODUCT_TM=75.4
PRIMER_PAIR_15_PENALTY=25.352875
PRIMER_LEFT_15_PENALTY=7.193142
PRIMER_RIGHT_15_PENALTY=18.159733
PRIMER_LEFT_15_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
PRIMER_RIGHT_15_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_15=177,23
PRIMER_RIGHT_15=3222,27
PRIMER_LEFT_15_TM=64.193
PRIMER_RIGHT_15_TM=57.840
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=25.926
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
PRIMER_RIGHT_15_POSITION_PENALTY=9.000000
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=3046
PRIMER_PAIR_15_PRODUCT_TM=75.4
PRIMER_PAIR_16_PENALTY=25.615737
PRIMER_LEFT_16_PENALTY=8.236744
PRIMER_RIGHT_16_PENALTY=17.378993
PRIMER_LEFT_16_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
PRIMER_RIGHT_16_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
PRIMER_LEFT_16=177,24
PRIMER_RIGHT_16=3221,26
PRIMER_LEFT_16_TM=64.237
PRIMER_RIGHT_16_TM=57.121
PRIMER_LEFT_16_GC_PERCENT=45.833
PRIMER_RIGHT_16_GC_PERCENT=26.923
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
PRIMER_RIGHT_16_POSITION_PENALTY=8.500000
PRIMER_LEFT_16_END_STABILITY=9.3000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=3045
PRIMER_PAIR_16_PRODUCT_TM=75.4
PRIMER_PAIR_17_PENALTY=25.667366
PRIMER_LEFT_17_PENALTY=1.996678
PRIMER_RIGHT_17_PENALTY=23.670687
PRIMER_LEFT_17_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_17_SEQUENCE=TAGTCTTCTTACTAAAATCCCACAAAT
PRIMER_LEFT_17=177,21
PRIMER_RIGHT_17=3232,27
PRIMER_LEFT_17_TM=59.003
PRIMER_RIGHT_17_TM=57.329
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=29.630
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
PRIMER_RIGHT_17_POSITION_PENALTY=14.000000
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=3056
PRIMER_PAIR_17_PRODUCT_TM=75.4
PRIMER_PAIR_18_PENALTY=25.768931
PRIMER_LEFT_18_PENALTY=1.996678
PRIMER_RIGHT_18_PENALTY=23.772253
PRIMER_LEFT_18_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
PRIMER_RIGHT_18_SEQUENCE=GTAGTCTTCTTACTAAAATCCCACAAA
PRIMER_LEFT_18=177,21
PRIMER_RIGHT_18=3233,27
PRIMER_LEFT_18_TM=59.003
PRIMER_RIGHT_18_TM=57.728
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=33.333
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
PRIMER_RIGHT_18_POSITION_PENALTY=14.500000
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=3057
PRIMER_PAIR_18_PRODUCT_TM=75.4
PRIMER_PAIR_19_PENALTY=26.287746
PRIMER_LEFT_19_PENALTY=8.236744
PRIMER_RIGHT_19_PENALTY=18.051002
PRIMER_LEFT_19_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
PRIMER_RIGHT_19_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
PRIMER_LEFT_19=177,24
PRIMER_RIGHT_19=3221,27
PRIMER_LEFT_19_TM=64.237
PRIMER_RIGHT_19_TM=57.449
PRIMER_LEFT_19_GC_PERCENT=45.833
PRIMER_RIGHT_19_GC_PERCENT=25.926
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
PRIMER_RIGHT_19_POSITION_PENALTY=8.500000
PRIMER_LEFT_19_END_STABILITY=9.3000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=3045
PRIMER_PAIR_19_PRODUCT_TM=75.4
=
