PRIMER_DNTP_CONC=0.0
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_DIVALENT=1.5
SEQUENCE_ID=exon1
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
SEQUENCE_EXCLUDED_REGION=100,150
PRIMER_PRODUCT_SIZE_RANGE=200-250
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, high tm 224, high hairpin stability 11, ok 543
PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 523, high repeat similarity 1, ok 386
PRIMER_PAIR_EXPLAIN=considered 242, unacceptable product size 236, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.082008
PRIMER_LEFT_0_PENALTY=3.208781
PRIMER_RIGHT_0_PENALTY=4.873227
PRIMER_LEFT_0_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_0=38,18
PRIMER_RIGHT_0=279,18
PRIMER_LEFT_0_TM=56.209
PRIMER_RIGHT_0_TM=57.873
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=39.48
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=3.5100
PRIMER_RIGHT_0_END_STABILITY=4.0200
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=242
PRIMER_PAIR_0_PRODUCT_TM=92.6
PRIMER_PAIR_1_PENALTY=8.431078
PRIMER_LEFT_1_PENALTY=3.557851
PRIMER_RIGHT_1_PENALTY=4.873227
PRIMER_LEFT_1_SEQUENCE=AGTTTCACCCACGAGTGA
PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_1=39,18
PRIMER_RIGHT_1=279,18
PRIMER_LEFT_1_TM=56.558
PRIMER_RIGHT_1_TM=57.873
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY_TH=5.03
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.42
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=3.4100
PRIMER_RIGHT_1_END_STABILITY=4.0200
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=241
PRIMER_PAIR_1_PRODUCT_TM=92.5
PRIMER_PAIR_2_PENALTY=8.634200
PRIMER_LEFT_2_PENALTY=3.760974
PRIMER_RIGHT_2_PENALTY=4.873227
PRIMER_LEFT_2_SEQUENCE=ACCCACGAGTGAAGACTC
PRIMER_RIGHT_2_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_2=45,18
PRIMER_RIGHT_2=279,18
PRIMER_LEFT_2_TM=56.761
PRIMER_RIGHT_2_TM=57.873
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY_TH=5.03
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=45.16
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=3.3600
PRIMER_RIGHT_2_END_STABILITY=4.0200
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=235
PRIMER_PAIR_2_PRODUCT_TM=92.7
PRIMER_PAIR_3_PENALTY=8.796608
PRIMER_LEFT_3_PENALTY=3.923381
PRIMER_RIGHT_3_PENALTY=4.873227
PRIMER_LEFT_3_SEQUENCE=GAGTTTCACCCACGAGTGA
PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_LEFT_3=38,19
PRIMER_RIGHT_3=279,18
PRIMER_LEFT_3_TM=57.923
PRIMER_RIGHT_3_TM=57.873
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY_TH=5.03
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=45.42
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.4100
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=242
PRIMER_PAIR_3_PRODUCT_TM=92.6
PRIMER_PAIR_4_PENALTY=8.810255
PRIMER_LEFT_4_PENALTY=3.208781
PRIMER_RIGHT_4_PENALTY=5.601474
PRIMER_LEFT_4_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC
PRIMER_LEFT_4=38,18
PRIMER_RIGHT_4=280,18
PRIMER_LEFT_4_TM=56.209
PRIMER_RIGHT_4_TM=58.601
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=39.48
PRIMER_RIGHT_4_HAIRPIN_TH=40.78
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=3.5100
PRIMER_RIGHT_4_END_STABILITY=3.6700
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=243
PRIMER_PAIR_4_PRODUCT_TM=92.6
=
SEQUENCE_ID=exon2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high repeat similarity 1, long poly-x seq 23, ok 499
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 6, high repeat similarity 4, ok 264
PRIMER_PAIR_EXPLAIN=considered 188, unacceptable product size 179, tm diff too large 2, ok 7
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.475856
PRIMER_LEFT_0_PENALTY=0.096015
PRIMER_RIGHT_0_PENALTY=3.379841
PRIMER_LEFT_0_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_0=21,20
PRIMER_RIGHT_0=267,23
PRIMER_LEFT_0_TM=55.096
PRIMER_RIGHT_0_TM=54.620
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=2.4100
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=247
PRIMER_PAIR_0_PRODUCT_TM=84.0
PRIMER_PAIR_1_PENALTY=3.475856
PRIMER_LEFT_1_PENALTY=0.096015
PRIMER_RIGHT_1_PENALTY=3.379841
PRIMER_LEFT_1_SEQUENCE=CCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_1_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_1=20,20
PRIMER_RIGHT_1=267,23
PRIMER_LEFT_1_TM=55.096
PRIMER_RIGHT_1_TM=54.620
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=30.435
PRIMER_LEFT_1_SELF_ANY_TH=1.42
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=5.1900
PRIMER_RIGHT_1_END_STABILITY=2.4100
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=248
PRIMER_PAIR_1_PRODUCT_TM=84.1
PRIMER_PAIR_2_PENALTY=4.300910
PRIMER_LEFT_2_PENALTY=0.096015
PRIMER_RIGHT_2_PENALTY=4.204895
PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_2_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=268,24
PRIMER_LEFT_2_TM=55.096
PRIMER_RIGHT_2_TM=55.205
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=2.4100
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=248
PRIMER_PAIR_2_PRODUCT_TM=83.9
PRIMER_PAIR_3_PENALTY=4.300910
PRIMER_LEFT_3_PENALTY=0.096015
PRIMER_RIGHT_3_PENALTY=4.204895
PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_3_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=268,24
PRIMER_LEFT_3_TM=55.096
PRIMER_RIGHT_3_TM=55.205
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY_TH=1.42
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=5.1900
PRIMER_RIGHT_3_END_STABILITY=2.4100
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=249
PRIMER_PAIR_3_PRODUCT_TM=84.0
PRIMER_PAIR_4_PENALTY=4.305829
PRIMER_LEFT_4_PENALTY=0.096015
PRIMER_RIGHT_4_PENALTY=4.209814
PRIMER_LEFT_4_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_4_SEQUENCE=GATGTCTACTTTCCAAATTACA
PRIMER_LEFT_4=21,20
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=55.096
PRIMER_RIGHT_4_TM=52.790
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=31.818
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=2.4100
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=246
PRIMER_PAIR_4_PRODUCT_TM=84.0
=
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_ID=exon2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high repeat similarity 1, long poly-x seq 23, ok 499
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 6, high repeat similarity 4, ok 264
PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 1417, long poly-x seq 23, ok 1323
PRIMER_PAIR_EXPLAIN=considered 188, unacceptable product size 179, tm diff too large 2, ok 7
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.475856
PRIMER_LEFT_0_PENALTY=0.096015
PRIMER_RIGHT_0_PENALTY=3.379841
PRIMER_INTERNAL_0_PENALTY=0.542784
PRIMER_LEFT_0_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_0_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_0=21,20
PRIMER_RIGHT_0=267,23
PRIMER_INTERNAL_0=181,20
PRIMER_LEFT_0_TM=55.096
PRIMER_RIGHT_0_TM=54.620
PRIMER_INTERNAL_0_TM=59.457
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_INTERNAL_0_GC_PERCENT=65.000
PRIMER_INTERNAL_0_SELF_ANY_TH=1.15
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=27.37
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=2.4100
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=247
PRIMER_PAIR_0_PRODUCT_TM=84.0
PRIMER_PAIR_1_PENALTY=3.475856
PRIMER_LEFT_1_PENALTY=0.096015
PRIMER_RIGHT_1_PENALTY=3.379841
PRIMER_INTERNAL_1_PENALTY=0.542784
PRIMER_LEFT_1_SEQUENCE=CCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_1_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_1_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_1=20,20
PRIMER_RIGHT_1=267,23
PRIMER_INTERNAL_1=181,20
PRIMER_LEFT_1_TM=55.096
PRIMER_RIGHT_1_TM=54.620
PRIMER_INTERNAL_1_TM=59.457
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=30.435
PRIMER_INTERNAL_1_GC_PERCENT=65.000
PRIMER_INTERNAL_1_SELF_ANY_TH=1.15
PRIMER_LEFT_1_SELF_ANY_TH=1.42
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=27.37
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=5.1900
PRIMER_RIGHT_1_END_STABILITY=2.4100
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=248
PRIMER_PAIR_1_PRODUCT_TM=84.1
PRIMER_PAIR_2_PENALTY=4.300910
PRIMER_LEFT_2_PENALTY=0.096015
PRIMER_RIGHT_2_PENALTY=4.204895
PRIMER_INTERNAL_2_PENALTY=0.542784
PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_2_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_2_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=268,24
PRIMER_INTERNAL_2=181,20
PRIMER_LEFT_2_TM=55.096
PRIMER_RIGHT_2_TM=55.205
PRIMER_INTERNAL_2_TM=59.457
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=29.167
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=1.15
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=27.37
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=2.4100
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=248
PRIMER_PAIR_2_PRODUCT_TM=83.9
PRIMER_PAIR_3_PENALTY=4.300910
PRIMER_LEFT_3_PENALTY=0.096015
PRIMER_RIGHT_3_PENALTY=4.204895
PRIMER_INTERNAL_3_PENALTY=0.542784
PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC
PRIMER_RIGHT_3_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_3_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=268,24
PRIMER_INTERNAL_3=181,20
PRIMER_LEFT_3_TM=55.096
PRIMER_RIGHT_3_TM=55.205
PRIMER_INTERNAL_3_TM=59.457
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=29.167
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=1.15
PRIMER_LEFT_3_SELF_ANY_TH=1.42
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=27.37
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=5.1900
PRIMER_RIGHT_3_END_STABILITY=2.4100
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=249
PRIMER_PAIR_3_PRODUCT_TM=84.0
PRIMER_PAIR_4_PENALTY=4.305829
PRIMER_LEFT_4_PENALTY=0.096015
PRIMER_RIGHT_4_PENALTY=4.209814
PRIMER_INTERNAL_4_PENALTY=0.542784
PRIMER_LEFT_4_SEQUENCE=CTTAAGTTTGTAACAGGGCC
PRIMER_RIGHT_4_SEQUENCE=GATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_4_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_4=21,20
PRIMER_RIGHT_4=266,22
PRIMER_INTERNAL_4=181,20
PRIMER_LEFT_4_TM=55.096
PRIMER_RIGHT_4_TM=52.790
PRIMER_INTERNAL_4_TM=59.457
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=31.818
PRIMER_INTERNAL_4_GC_PERCENT=65.000
PRIMER_INTERNAL_4_SELF_ANY_TH=1.15
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=27.37
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=2.4100
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=246
PRIMER_PAIR_4_PRODUCT_TM=84.0
=
SEQUENCE_ID=exon1_2
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
SEQUENCE_EXCLUDED_REGION=100,150
PRIMER_PRODUCT_SIZE_RANGE=200-250
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, high tm 224, high hairpin stability 11, ok 543
PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 523, high repeat similarity 1, ok 386
PRIMER_INTERNAL_EXPLAIN=considered 3237, too many Ns 27, GC content failed 32, low tm 155, high tm 94, high hairpin stability 1, ok 2928
PRIMER_PAIR_EXPLAIN=considered 304, unacceptable product size 293, high mispriming library similarity 3, ok 8
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.082008
PRIMER_LEFT_0_PENALTY=3.208781
PRIMER_RIGHT_0_PENALTY=4.873227
PRIMER_INTERNAL_0_PENALTY=0.026924
PRIMER_LEFT_0_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_INTERNAL_0_SEQUENCE=AGCAGAGGCTGTGCTTGGCG
PRIMER_LEFT_0=38,18
PRIMER_RIGHT_0=279,18
PRIMER_INTERNAL_0=142,20
PRIMER_LEFT_0_TM=56.209
PRIMER_RIGHT_0_TM=57.873
PRIMER_INTERNAL_0_TM=59.973
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_INTERNAL_0_GC_PERCENT=65.000
PRIMER_INTERNAL_0_SELF_ANY_TH=25.56
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=39.48
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=43.71
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=3.5100
PRIMER_RIGHT_0_END_STABILITY=4.0200
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=242
PRIMER_PAIR_0_PRODUCT_TM=92.6
PRIMER_PAIR_1_PENALTY=8.634200
PRIMER_LEFT_1_PENALTY=3.760974
PRIMER_RIGHT_1_PENALTY=4.873227
PRIMER_INTERNAL_1_PENALTY=0.026924
PRIMER_LEFT_1_SEQUENCE=ACCCACGAGTGAAGACTC
PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_INTERNAL_1_SEQUENCE=AGCAGAGGCTGTGCTTGGCG
PRIMER_LEFT_1=45,18
PRIMER_RIGHT_1=279,18
PRIMER_INTERNAL_1=142,20
PRIMER_LEFT_1_TM=56.761
PRIMER_RIGHT_1_TM=57.873
PRIMER_INTERNAL_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=55.556
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_INTERNAL_1_GC_PERCENT=65.000
PRIMER_INTERNAL_1_SELF_ANY_TH=25.56
PRIMER_LEFT_1_SELF_ANY_TH=5.03
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.16
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=43.71
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=3.3600
PRIMER_RIGHT_1_END_STABILITY=4.0200
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=235
PRIMER_PAIR_1_PRODUCT_TM=92.7
PRIMER_PAIR_2_PENALTY=8.810255
PRIMER_LEFT_2_PENALTY=3.208781
PRIMER_RIGHT_2_PENALTY=5.601474
PRIMER_INTERNAL_2_PENALTY=0.026924
PRIMER_LEFT_2_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_2_SEQUENCE=GCTCCTCAGCCTTGTCAC
PRIMER_INTERNAL_2_SEQUENCE=AGCAGAGGCTGTGCTTGGCG
PRIMER_LEFT_2=38,18
PRIMER_RIGHT_2=280,18
PRIMER_INTERNAL_2=142,20
PRIMER_LEFT_2_TM=56.209
PRIMER_RIGHT_2_TM=58.601
PRIMER_INTERNAL_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=25.56
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=39.48
PRIMER_RIGHT_2_HAIRPIN_TH=40.78
PRIMER_INTERNAL_2_HAIRPIN_TH=43.71
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=3.5100
PRIMER_RIGHT_2_END_STABILITY=3.6700
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=243
PRIMER_PAIR_2_PRODUCT_TM=92.6
PRIMER_PAIR_3_PENALTY=8.954573
PRIMER_LEFT_3_PENALTY=4.081347
PRIMER_RIGHT_3_PENALTY=4.873227
PRIMER_INTERNAL_3_PENALTY=0.026924
PRIMER_LEFT_3_SEQUENCE=CACCCACGAGTGAAGACT
PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC
PRIMER_INTERNAL_3_SEQUENCE=AGCAGAGGCTGTGCTTGGCG
PRIMER_LEFT_3=44,18
PRIMER_RIGHT_3=279,18
PRIMER_INTERNAL_3=142,20
PRIMER_LEFT_3_TM=57.081
PRIMER_RIGHT_3_TM=57.873
PRIMER_INTERNAL_3_TM=59.973
PRIMER_LEFT_3_GC_PERCENT=55.556
PRIMER_RIGHT_3_GC_PERCENT=61.111
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=25.56
PRIMER_LEFT_3_SELF_ANY_TH=5.03
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=40.95
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=43.71
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.2400
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=236
PRIMER_PAIR_3_PRODUCT_TM=92.8
PRIMER_PAIR_4_PENALTY=9.128122
PRIMER_LEFT_4_PENALTY=3.208781
PRIMER_RIGHT_4_PENALTY=5.919341
PRIMER_INTERNAL_4_PENALTY=0.026924
PRIMER_LEFT_4_SEQUENCE=GAGTTTCACCCACGAGTG
PRIMER_RIGHT_4_SEQUENCE=CTTGTCACCCGCTCTTGG
PRIMER_INTERNAL_4_SEQUENCE=AGCAGAGGCTGTGCTTGGCG
PRIMER_LEFT_4=38,18
PRIMER_RIGHT_4=270,18
PRIMER_INTERNAL_4=142,20
PRIMER_LEFT_4_TM=56.209
PRIMER_RIGHT_4_TM=58.919
PRIMER_INTERNAL_4_TM=59.973
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_INTERNAL_4_GC_PERCENT=65.000
PRIMER_INTERNAL_4_SELF_ANY_TH=25.56
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=39.48
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=43.71
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=3.5100
PRIMER_RIGHT_4_END_STABILITY=3.6100
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=233
PRIMER_PAIR_4_PRODUCT_TM=92.4
=
SEQUENCE_ID=exon2_2
PRIMER_NUM_RETURN=7
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high repeat similarity 2, long poly-x seq 23, ok 498
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 6, high repeat similarity 8, ok 260
PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 1417, long poly-x seq 23, ok 1323
PRIMER_PAIR_EXPLAIN=considered 495, unacceptable product size 446, tm diff too large 24, high mispriming library similarity 17, ok 8
PRIMER_LEFT_NUM_RETURNED=7
PRIMER_RIGHT_NUM_RETURNED=7
PRIMER_INTERNAL_NUM_RETURNED=7
PRIMER_PAIR_NUM_RETURNED=7
PRIMER_PAIR_0_PENALTY=4.767068
PRIMER_LEFT_0_PENALTY=1.387227
PRIMER_RIGHT_0_PENALTY=3.379841
PRIMER_INTERNAL_0_PENALTY=0.542784
PRIMER_LEFT_0_SEQUENCE=ACCTTAAGTTTGTAACAGGG
PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_0_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_0=19,20
PRIMER_RIGHT_0=267,23
PRIMER_INTERNAL_0=181,20
PRIMER_LEFT_0_TM=53.613
PRIMER_RIGHT_0_TM=54.620
PRIMER_INTERNAL_0_TM=59.457
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_INTERNAL_0_GC_PERCENT=65.000
PRIMER_INTERNAL_0_SELF_ANY_TH=1.15
PRIMER_LEFT_0_SELF_ANY_TH=1.42
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=27.37
PRIMER_LEFT_0_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=4.4500
PRIMER_RIGHT_0_END_STABILITY=2.4100
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=249
PRIMER_PAIR_0_PRODUCT_TM=84.0
PRIMER_PAIR_1_PENALTY=4.829150
PRIMER_LEFT_1_PENALTY=1.449308
PRIMER_RIGHT_1_PENALTY=3.379841
PRIMER_INTERNAL_1_PENALTY=0.542784
PRIMER_LEFT_1_SEQUENCE=AACCTTAAGTTTGTAACAGGG
PRIMER_RIGHT_1_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_1_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_1=18,21
PRIMER_RIGHT_1=267,23
PRIMER_INTERNAL_1=181,20
PRIMER_LEFT_1_TM=54.551
PRIMER_RIGHT_1_TM=54.620
PRIMER_INTERNAL_1_TM=59.457
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=30.435
PRIMER_INTERNAL_1_GC_PERCENT=65.000
PRIMER_INTERNAL_1_SELF_ANY_TH=1.15
PRIMER_LEFT_1_SELF_ANY_TH=1.42
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=34.81
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=27.37
PRIMER_LEFT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=4.4500
PRIMER_RIGHT_1_END_STABILITY=2.4100
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=250
PRIMER_PAIR_1_PRODUCT_TM=84.0
PRIMER_PAIR_2_PENALTY=4.841351
PRIMER_LEFT_2_PENALTY=1.461510
PRIMER_RIGHT_2_PENALTY=3.379841
PRIMER_INTERNAL_2_PENALTY=0.542784
PRIMER_LEFT_2_SEQUENCE=TTAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_2_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_2=22,20
PRIMER_RIGHT_2=267,23
PRIMER_INTERNAL_2=181,20
PRIMER_LEFT_2_TM=56.462
PRIMER_RIGHT_2_TM=54.620
PRIMER_INTERNAL_2_TM=59.457
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=1.15
PRIMER_LEFT_2_SELF_ANY_TH=17.05
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=17.05
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=27.37
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=2.4100
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=246
PRIMER_PAIR_2_PRODUCT_TM=83.8
PRIMER_PAIR_3_PENALTY=4.956287
PRIMER_LEFT_3_PENALTY=1.576445
PRIMER_RIGHT_3_PENALTY=3.379841
PRIMER_INTERNAL_3_PENALTY=0.542784
PRIMER_LEFT_3_SEQUENCE=TAAGTTTGTAACAGGGCCC
PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_3_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_3=23,19
PRIMER_RIGHT_3=267,23
PRIMER_INTERNAL_3=181,20
PRIMER_LEFT_3_TM=55.576
PRIMER_RIGHT_3_TM=54.620
PRIMER_INTERNAL_3_TM=59.457
PRIMER_LEFT_3_GC_PERCENT=47.368
PRIMER_RIGHT_3_GC_PERCENT=30.435
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=1.15
PRIMER_LEFT_3_SELF_ANY_TH=17.05
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=17.05
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=27.37
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=2.4100
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=245
PRIMER_PAIR_3_PRODUCT_TM=83.9
PRIMER_PAIR_4_PENALTY=5.121610
PRIMER_LEFT_4_PENALTY=1.741769
PRIMER_RIGHT_4_PENALTY=3.379841
PRIMER_INTERNAL_4_PENALTY=0.542784
PRIMER_LEFT_4_SEQUENCE=TTTGTAACAGGGCCCAAAT
PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_4_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_4=27,19
PRIMER_RIGHT_4=267,23
PRIMER_INTERNAL_4=181,20
PRIMER_LEFT_4_TM=55.742
PRIMER_RIGHT_4_TM=54.620
PRIMER_INTERNAL_4_TM=59.457
PRIMER_LEFT_4_GC_PERCENT=42.105
PRIMER_RIGHT_4_GC_PERCENT=30.435
PRIMER_INTERNAL_4_GC_PERCENT=65.000
PRIMER_INTERNAL_4_SELF_ANY_TH=1.15
PRIMER_LEFT_4_SELF_ANY_TH=28.06
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=12.33
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=27.37
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=2.3200
PRIMER_RIGHT_4_END_STABILITY=2.4100
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=241
PRIMER_PAIR_4_PRODUCT_TM=84.0
PRIMER_PAIR_5_PENALTY=5.592121
PRIMER_LEFT_5_PENALTY=1.387227
PRIMER_RIGHT_5_PENALTY=4.204895
PRIMER_INTERNAL_5_PENALTY=0.542784
PRIMER_LEFT_5_SEQUENCE=ACCTTAAGTTTGTAACAGGG
PRIMER_RIGHT_5_SEQUENCE=ATGATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_5_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_5=19,20
PRIMER_RIGHT_5=268,24
PRIMER_INTERNAL_5=181,20
PRIMER_LEFT_5_TM=53.613
PRIMER_RIGHT_5_TM=55.205
PRIMER_INTERNAL_5_TM=59.457
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=29.167
PRIMER_INTERNAL_5_GC_PERCENT=65.000
PRIMER_INTERNAL_5_SELF_ANY_TH=1.15
PRIMER_LEFT_5_SELF_ANY_TH=1.42
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=27.37
PRIMER_LEFT_5_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=4.4500
PRIMER_RIGHT_5_END_STABILITY=2.4100
PRIMER_PAIR_5_COMPL_ANY_TH=0.00
PRIMER_PAIR_5_COMPL_END_TH=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=250
PRIMER_PAIR_5_PRODUCT_TM=84.0
PRIMER_PAIR_6_PENALTY=5.597041
PRIMER_LEFT_6_PENALTY=1.387227
PRIMER_RIGHT_6_PENALTY=4.209814
PRIMER_INTERNAL_6_PENALTY=0.542784
PRIMER_LEFT_6_SEQUENCE=ACCTTAAGTTTGTAACAGGG
PRIMER_RIGHT_6_SEQUENCE=GATGTCTACTTTCCAAATTACA
PRIMER_INTERNAL_6_SEQUENCE=TGAGGACTGGGTGCTGGCCT
PRIMER_LEFT_6=19,20
PRIMER_RIGHT_6=266,22
PRIMER_INTERNAL_6=181,20
PRIMER_LEFT_6_TM=53.613
PRIMER_RIGHT_6_TM=52.790
PRIMER_INTERNAL_6_TM=59.457
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=31.818
PRIMER_INTERNAL_6_GC_PERCENT=65.000
PRIMER_INTERNAL_6_SELF_ANY_TH=1.15
PRIMER_LEFT_6_SELF_ANY_TH=1.42
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=27.37
PRIMER_LEFT_6_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=4.4500
PRIMER_RIGHT_6_END_STABILITY=2.4100
PRIMER_PAIR_6_COMPL_ANY_TH=0.00
PRIMER_PAIR_6_COMPL_END_TH=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=248
PRIMER_PAIR_6_PRODUCT_TM=84.1
=
PRIMER_NUM_RETURN=5
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_ID=exon2_3
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high hairpin stability 6, high repeat similarity 153, long poly-x seq 23, ok 341
PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 97, high repeat similarity 126, ok 51
PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 1417, long poly-x seq 23, ok 1323
PRIMER_PAIR_EXPLAIN=considered 20759, unacceptable product size 11888, tm diff too large 5556, high mispriming library similarity 3315, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
