PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_SALT_DIVALENT=0.0
PRIMER_DNTP_CONC=0.0
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
SEQUENCE_ID=thermod_default
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=primer3_config/
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_ANY_TH=47.0
PRIMER_MAX_SELF_END_TH=47.0
PRIMER_MAX_HAIRPIN_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0
PRIMER_PAIR_MAX_COMPL_END_TH=47.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0
PRIMER_INTERNAL_MAX_SELF_END_TH=47.0
PRIMER_INTERNAL_MAX_HAIRPIN_TH=47.0
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.0
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=47.0
PRIMER_WT_SELF_ANY_TH=0.0
PRIMER_WT_SELF_END_TH=0.0
PRIMER_WT_HAIRPIN_TH=0.0
PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0
PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0
PRIMER_INTERNAL_WT_SELF_END_TH=0.0
PRIMER_INTERNAL_WT_HAIRPIN_TH=0.0
PRIMER_PAIR_WT_COMPL_ANY_TH=0.0
PRIMER_PAIR_WT_COMPL_END_TH=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=0.0
P3_COMMENT=default values of parameters using thermodynamic approach
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
=
SEQUENCE_ID=any_1
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_ANY_TH=10.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0
PRIMER_WT_SELF_ANY_TH=0.0
PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0
PRIMER_PAIR_WT_COMPL_ANY_TH=0.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=any_salt
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_DIVALENT=2.5
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_ANY_TH=60.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0
PRIMER_WT_SELF_ANY_TH=0.0
PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0
PRIMER_PAIR_WT_COMPL_ANY_TH=0.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=any_weight
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_DIVALENT=2.5
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_ANY_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0
PRIMER_WT_SELF_ANY_TH=1.0
PRIMER_INTERNAL_WT_SELF_ANY_TH=0.1
PRIMER_PAIR_WT_COMPL_ANY_TH=0.7
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=any_2
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_DIVALENT=2.5
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_ANY_TH=5.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=5.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH=5.0
PRIMER_WT_SELF_ANY_TH=1.0
PRIMER_INTERNAL_WT_SELF_ANY_TH=1.0
PRIMER_PAIR_WT_COMPL_ANY_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=any_no_effect
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_DIVALENT=2.5
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_ANY_TH=0.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=0.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH=0.0
PRIMER_WT_SELF_ANY_TH=0.0
PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0
PRIMER_PAIR_WT_COMPL_ANY_TH=0.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=end_th1
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_END_TH=10.0
PRIMER_PAIR_MAX_COMPL_END_TH=10.0
PRIMER_INTERNAL_MAX_SELF_END_TH=10.0
PRIMER_WT_SELF_END_TH=0.0
PRIMER_INTERNAL_WT_SELF_END_TH=0.0
PRIMER_PAIR_WT_COMPL_END_TH=0.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=end_th2
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_SELF_END_TH=47.0
PRIMER_PAIR_MAX_COMPL_END_TH=47.0
PRIMER_INTERNAL_MAX_SELF_END_TH=47.0
PRIMER_WT_SELF_END_TH=0.1
PRIMER_INTERNAL_WT_SELF_END_TH=0.01
PRIMER_PAIR_WT_COMPL_END_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=hairpin1
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_HAIRPIN_TH=50.0
PRIMER_INTERNAL_MAX_HAIRPIN_TH=50.0
PRIMER_WT_HAIRPIN_TH=1.0
PRIMER_INTERNAL_WT_HAIRPIN_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=hairpin2
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_HAIRPIN_TH=40.0
PRIMER_INTERNAL_MAX_HAIRPIN_TH=40.0
PRIMER_WT_HAIRPIN_TH=0.0
PRIMER_INTERNAL_WT_HAIRPIN_TH=0.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=hairpin3
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_HAIRPIN_TH=0.0
PRIMER_INTERNAL_MAX_HAIRPIN_TH=47.0
PRIMER_PICK_ANYWAY=1
PRIMER_WT_HAIRPIN_TH=0.0
PRIMER_INTERNAL_WT_HAIRPIN_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=template1
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=45.0
PRIMER_WT_TEMPLATE_MISPRIMING_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=template2
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=50.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=template2_and_repeat_library
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_MISPRIMING_LIBRARY=../src/humrep_and_simple.txt
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=50.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=1.0
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
SEQUENCE_ID=template3
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0
PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=5
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=20
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
=
