PRIMER_NUM_RETURN=2
PRIMER_OPT_SIZE=20
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_MAX_POLY_X=100
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
PRIMER_MAX_GC=80.0
PRIMER_MAX_SIZE=25
PRIMER_MAX_TM=63.0
PRIMER_MIN_SIZE=18
PRIMER_MIN_GC=20
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=6
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=6
SEQUENCE_ID=01.RHAP33FA1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=20,315
SEQUENCE_TARGET=81,30
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.733854
PRIMER_LEFT_0_PENALTY=7.538496
PRIMER_RIGHT_0_PENALTY=0.195358
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_0_SEQUENCE=GGCAAAAGGACAGAGGATGA
PRIMER_LEFT_0=20,25
PRIMER_RIGHT_0=177,20
PRIMER_LEFT_0_TM=57.462
PRIMER_RIGHT_0_TM=60.195
PRIMER_LEFT_0_GC_PERCENT=32.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=158
PRIMER_PAIR_0_PRODUCT_TM=66.7
PRIMER_PAIR_1_PENALTY=7.868046
PRIMER_LEFT_1_PENALTY=7.538496
PRIMER_RIGHT_1_PENALTY=0.329550
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
PRIMER_LEFT_1=20,25
PRIMER_RIGHT_1=183,20
PRIMER_LEFT_1_TM=57.462
PRIMER_RIGHT_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=164
PRIMER_PAIR_1_PRODUCT_TM=67.0
=
SEQUENCE_ID=01.RHAP34FA1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
SEQUENCE_INCLUDED_REGION=19,296
SEQUENCE_TARGET=134,15
SEQUENCE_TARGET=213,16
SEQUENCE_TARGET=235,16
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_NUM_RETURN=1
SEQUENCE_ID=03.RHAP34FA3.seq
SEQUENCE_TEMPLATE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
SEQUENCE_INCLUDED_REGION=0,278
SEQUENCE_TARGET=1,28
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=03.RHAP34FE3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
SEQUENCE_INCLUDED_REGION=17,284
SEQUENCE_TARGET=27,60
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=04.RHAP33FE4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
SEQUENCE_INCLUDED_REGION=18,367
SEQUENCE_TARGET=146,46
SEQUENCE_TARGET=199,22
SEQUENCE_TARGET=286,13
SEQUENCE_TARGET=300,15
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.353468
PRIMER_LEFT_0_PENALTY=4.174047
PRIMER_RIGHT_0_PENALTY=3.179421
PRIMER_LEFT_0_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
PRIMER_RIGHT_0_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT_0=97,24
PRIMER_RIGHT_0=270,23
PRIMER_LEFT_0_TM=60.174
PRIMER_RIGHT_0_TM=59.821
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=67.3
=
SEQUENCE_ID=05.RHAP33FA5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
SEQUENCE_INCLUDED_REGION=10,369
SEQUENCE_TARGET=30,44
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=05.RHAP33FE5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=14,306
SEQUENCE_TARGET=176,34
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.858517
PRIMER_LEFT_0_PENALTY=0.702456
PRIMER_RIGHT_0_PENALTY=0.156061
PRIMER_LEFT_0_SEQUENCE=CATGTTCGTGCAAACACTCA
PRIMER_RIGHT_0_SEQUENCE=AGACCCTGTCTCCAAAAGCA
PRIMER_LEFT_0=129,20
PRIMER_RIGHT_0=302,20
PRIMER_LEFT_0_TM=59.298
PRIMER_RIGHT_0_TM=59.844
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=66.1
=
PRIMER_NUM_RETURN=40
SEQUENCE_ID=05.RHAP34FA5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
SEQUENCE_INCLUDED_REGION=9,297
SEQUENCE_TARGET=125,36
SEQUENCE_TARGET=162,24
SEQUENCE_TARGET=191,16
SEQUENCE_TARGET=214,14
PRIMER_LEFT_NUM_RETURNED=40
PRIMER_RIGHT_NUM_RETURNED=40
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=40
PRIMER_PAIR_0_PENALTY=9.884342
PRIMER_LEFT_0_PENALTY=1.973952
PRIMER_RIGHT_0_PENALTY=7.910390
PRIMER_LEFT_0_SEQUENCE=GCGGCCGACATCTACTACTA
PRIMER_RIGHT_0_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_0=103,20
PRIMER_RIGHT_0=268,25
PRIMER_LEFT_0_TM=58.026
PRIMER_RIGHT_0_TM=57.090
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=44.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=4.7000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=166
PRIMER_PAIR_0_PRODUCT_TM=65.2
PRIMER_PAIR_1_PENALTY=10.012256
PRIMER_LEFT_1_PENALTY=2.101866
PRIMER_RIGHT_1_PENALTY=7.910390
PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_1=103,21
PRIMER_RIGHT_1=268,25
PRIMER_LEFT_1_TM=58.898
PRIMER_RIGHT_1_TM=57.090
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=166
PRIMER_PAIR_1_PRODUCT_TM=65.2
PRIMER_PAIR_2_PENALTY=10.079221
PRIMER_LEFT_2_PENALTY=2.168831
PRIMER_RIGHT_2_PENALTY=7.910390
PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=268,25
PRIMER_LEFT_2_TM=59.831
PRIMER_RIGHT_2_TM=57.090
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=44.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=65.2
PRIMER_PAIR_3_PENALTY=10.514211
PRIMER_LEFT_3_PENALTY=2.603822
PRIMER_RIGHT_3_PENALTY=7.910390
PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_3=102,20
PRIMER_RIGHT_3=268,25
PRIMER_LEFT_3_TM=62.604
PRIMER_RIGHT_3_TM=57.090
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=44.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=65.4
PRIMER_PAIR_4_PENALTY=10.656616
PRIMER_LEFT_4_PENALTY=2.746227
PRIMER_RIGHT_4_PENALTY=7.910390
PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_4=102,19
PRIMER_RIGHT_4=268,25
PRIMER_LEFT_4_TM=61.746
PRIMER_RIGHT_4_TM=57.090
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=44.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=167
PRIMER_PAIR_4_PRODUCT_TM=65.4
PRIMER_PAIR_5_PENALTY=10.846735
PRIMER_LEFT_5_PENALTY=2.936346
PRIMER_RIGHT_5_PENALTY=7.910390
PRIMER_LEFT_5_SEQUENCE=CGCGGCCGACATCTACTA
PRIMER_RIGHT_5_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_5=102,18
PRIMER_RIGHT_5=268,25
PRIMER_LEFT_5_TM=60.936
PRIMER_RIGHT_5_TM=57.090
PRIMER_LEFT_5_GC_PERCENT=61.111
PRIMER_RIGHT_5_GC_PERCENT=44.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=4.7000
PRIMER_RIGHT_5_END_STABILITY=6.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=167
PRIMER_PAIR_5_PRODUCT_TM=65.4
PRIMER_PAIR_6_PENALTY=10.974908
PRIMER_LEFT_6_PENALTY=3.064519
PRIMER_RIGHT_6_PENALTY=7.910390
PRIMER_LEFT_6_SEQUENCE=GCGGCCGACATCTACTACT
PRIMER_RIGHT_6_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_6=103,19
PRIMER_RIGHT_6=268,25
PRIMER_LEFT_6_TM=57.935
PRIMER_RIGHT_6_TM=57.090
PRIMER_LEFT_6_GC_PERCENT=57.895
PRIMER_RIGHT_6_GC_PERCENT=44.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.4000
PRIMER_RIGHT_6_END_STABILITY=6.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=166
PRIMER_PAIR_6_PRODUCT_TM=65.2
PRIMER_PAIR_7_PENALTY=11.429982
PRIMER_LEFT_7_PENALTY=3.519592
PRIMER_RIGHT_7_PENALTY=7.910390
PRIMER_LEFT_7_SEQUENCE=CGCGGCCGACATCTACTACTA
PRIMER_RIGHT_7_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_7=102,21
PRIMER_RIGHT_7=268,25
PRIMER_LEFT_7_TM=62.520
PRIMER_RIGHT_7_TM=57.090
PRIMER_LEFT_7_GC_PERCENT=57.143
PRIMER_RIGHT_7_GC_PERCENT=44.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=4.7000
PRIMER_RIGHT_7_END_STABILITY=6.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=167
PRIMER_PAIR_7_PRODUCT_TM=65.4
PRIMER_PAIR_8_PENALTY=0.347871
PRIMER_LEFT_8_PENALTY=0.003322
PRIMER_RIGHT_8_PENALTY=0.344549
PRIMER_LEFT_8_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_8_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_8=54,20
PRIMER_RIGHT_8=294,20
PRIMER_LEFT_8_TM=59.997
PRIMER_RIGHT_8_TM=60.345
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=241
PRIMER_PAIR_8_PRODUCT_TM=70.2
PRIMER_PAIR_9_PENALTY=0.387281
PRIMER_LEFT_9_PENALTY=0.042732
PRIMER_RIGHT_9_PENALTY=0.344549
PRIMER_LEFT_9_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_9_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_9=65,20
PRIMER_RIGHT_9=294,20
PRIMER_LEFT_9_TM=59.957
PRIMER_RIGHT_9_TM=60.345
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=230
PRIMER_PAIR_9_PRODUCT_TM=69.8
PRIMER_PAIR_10_PENALTY=0.478290
PRIMER_LEFT_10_PENALTY=0.133741
PRIMER_RIGHT_10_PENALTY=0.344549
PRIMER_LEFT_10_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_10_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_10=53,20
PRIMER_RIGHT_10=294,20
PRIMER_LEFT_10_TM=60.134
PRIMER_RIGHT_10_TM=60.345
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=4.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=242
PRIMER_PAIR_10_PRODUCT_TM=70.1
PRIMER_PAIR_11_PENALTY=0.478290
PRIMER_LEFT_11_PENALTY=0.133741
PRIMER_RIGHT_11_PENALTY=0.344549
PRIMER_LEFT_11_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_11_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_11=52,20
PRIMER_RIGHT_11=294,20
PRIMER_LEFT_11_TM=60.134
PRIMER_RIGHT_11_TM=60.345
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=4.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=243
PRIMER_PAIR_11_PRODUCT_TM=70.3
PRIMER_PAIR_12_PENALTY=0.605306
PRIMER_LEFT_12_PENALTY=0.260757
PRIMER_RIGHT_12_PENALTY=0.344549
PRIMER_LEFT_12_SEQUENCE=CCACTCTCATTCAGCCAGGT
PRIMER_RIGHT_12_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_12=70,20
PRIMER_RIGHT_12=294,20
PRIMER_LEFT_12_TM=60.261
PRIMER_RIGHT_12_TM=60.345
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=225
PRIMER_PAIR_12_PRODUCT_TM=69.4
PRIMER_PAIR_13_PENALTY=0.629670
PRIMER_LEFT_13_PENALTY=0.285121
PRIMER_RIGHT_13_PENALTY=0.344549
PRIMER_LEFT_13_SEQUENCE=GCAGCTGAGACTTTGAAGGC
PRIMER_RIGHT_13_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_13=51,20
PRIMER_RIGHT_13=294,20
PRIMER_LEFT_13_TM=60.285
PRIMER_RIGHT_13_TM=60.345
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=4.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=244
PRIMER_PAIR_13_PRODUCT_TM=70.4
PRIMER_PAIR_14_PENALTY=0.653951
PRIMER_LEFT_14_PENALTY=0.309402
PRIMER_RIGHT_14_PENALTY=0.344549
PRIMER_LEFT_14_SEQUENCE=CATTCAGCCAGGTGTGTGAG
PRIMER_RIGHT_14_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_14=77,20
PRIMER_RIGHT_14=294,20
PRIMER_LEFT_14_TM=60.309
PRIMER_RIGHT_14_TM=60.345
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=4.00
PRIMER_PAIR_14_PRODUCT_SIZE=218
PRIMER_PAIR_14_PRODUCT_TM=69.0
PRIMER_PAIR_15_PENALTY=0.653951
PRIMER_LEFT_15_PENALTY=0.309402
PRIMER_RIGHT_15_PENALTY=0.344549
PRIMER_LEFT_15_SEQUENCE=CTCATTCAGCCAGGTGTGTG
PRIMER_RIGHT_15_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_15=75,20
PRIMER_RIGHT_15=294,20
PRIMER_LEFT_15_TM=60.309
PRIMER_RIGHT_15_TM=60.345
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=4.00
PRIMER_PAIR_15_PRODUCT_SIZE=220
PRIMER_PAIR_15_PRODUCT_TM=69.1
PRIMER_PAIR_16_PENALTY=0.929938
PRIMER_LEFT_16_PENALTY=0.585389
PRIMER_RIGHT_16_PENALTY=0.344549
PRIMER_LEFT_16_SEQUENCE=CTCTCATTCAGCCAGGTGTG
PRIMER_RIGHT_16_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_16=73,20
PRIMER_RIGHT_16=294,20
PRIMER_LEFT_16_TM=59.415
PRIMER_RIGHT_16_TM=60.345
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=222
PRIMER_PAIR_16_PRODUCT_TM=69.2
PRIMER_PAIR_17_PENALTY=0.929938
PRIMER_LEFT_17_PENALTY=0.585389
PRIMER_RIGHT_17_PENALTY=0.344549
PRIMER_LEFT_17_SEQUENCE=CACTCTCATTCAGCCAGGTG
PRIMER_RIGHT_17_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_17=71,20
PRIMER_RIGHT_17=294,20
PRIMER_LEFT_17_TM=59.415
PRIMER_RIGHT_17_TM=60.345
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=224
PRIMER_PAIR_17_PRODUCT_TM=69.3
PRIMER_PAIR_18_PENALTY=0.975760
PRIMER_LEFT_18_PENALTY=0.631211
PRIMER_RIGHT_18_PENALTY=0.344549
PRIMER_LEFT_18_SEQUENCE=TTGAAGGCCACTCTCATTCA
PRIMER_RIGHT_18_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_18=63,20
PRIMER_RIGHT_18=294,20
PRIMER_LEFT_18_TM=59.369
PRIMER_RIGHT_18_TM=60.345
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=5.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=232
PRIMER_PAIR_18_PRODUCT_TM=69.7
PRIMER_PAIR_19_PENALTY=1.030805
PRIMER_LEFT_19_PENALTY=0.686256
PRIMER_RIGHT_19_PENALTY=0.344549
PRIMER_LEFT_19_SEQUENCE=TGCAGCTGAGACTTTGAAGG
PRIMER_RIGHT_19_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_19=50,20
PRIMER_RIGHT_19=294,20
PRIMER_LEFT_19_TM=59.314
PRIMER_RIGHT_19_TM=60.345
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=245
PRIMER_PAIR_19_PRODUCT_TM=70.3
PRIMER_PAIR_20_PENALTY=1.083301
PRIMER_LEFT_20_PENALTY=0.738752
PRIMER_RIGHT_20_PENALTY=0.344549
PRIMER_LEFT_20_SEQUENCE=TCTCATTCAGCCAGGTGTGT
PRIMER_RIGHT_20_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_20=74,20
PRIMER_RIGHT_20=294,20
PRIMER_LEFT_20_TM=59.261
PRIMER_RIGHT_20_TM=60.345
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=7.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=221
PRIMER_PAIR_20_PRODUCT_TM=69.1
PRIMER_PAIR_21_PENALTY=1.183339
PRIMER_LEFT_21_PENALTY=0.003322
PRIMER_RIGHT_21_PENALTY=1.180017
PRIMER_LEFT_21_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_21_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_21=54,20
PRIMER_RIGHT_21=290,20
PRIMER_LEFT_21_TM=59.997
PRIMER_RIGHT_21_TM=61.180
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=3.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=237
PRIMER_PAIR_21_PRODUCT_TM=70.2
PRIMER_PAIR_22_PENALTY=1.183339
PRIMER_LEFT_22_PENALTY=0.003322
PRIMER_RIGHT_22_PENALTY=1.180017
PRIMER_LEFT_22_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_22_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_22=54,20
PRIMER_RIGHT_22=292,20
PRIMER_LEFT_22_TM=59.997
PRIMER_RIGHT_22_TM=61.180
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=3.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=239
PRIMER_PAIR_22_PRODUCT_TM=70.3
PRIMER_PAIR_23_PENALTY=1.210181
PRIMER_LEFT_23_PENALTY=0.003322
PRIMER_RIGHT_23_PENALTY=1.206859
PRIMER_LEFT_23_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_23_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_23=54,20
PRIMER_RIGHT_23=290,19
PRIMER_LEFT_23_TM=59.997
PRIMER_RIGHT_23_TM=60.207
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=57.895
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=237
PRIMER_PAIR_23_PRODUCT_TM=70.2
PRIMER_PAIR_24_PENALTY=1.210181
PRIMER_LEFT_24_PENALTY=0.003322
PRIMER_RIGHT_24_PENALTY=1.206859
PRIMER_LEFT_24_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_24_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_24=54,20
PRIMER_RIGHT_24=291,19
PRIMER_LEFT_24_TM=59.997
PRIMER_RIGHT_24_TM=60.207
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=57.895
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=3.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=238
PRIMER_PAIR_24_PRODUCT_TM=70.3
PRIMER_PAIR_25_PENALTY=1.222749
PRIMER_LEFT_25_PENALTY=0.042732
PRIMER_RIGHT_25_PENALTY=1.180017
PRIMER_LEFT_25_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_25_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_25=65,20
PRIMER_RIGHT_25=290,20
PRIMER_LEFT_25_TM=59.957
PRIMER_RIGHT_25_TM=61.180
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=2.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=226
PRIMER_PAIR_25_PRODUCT_TM=69.8
PRIMER_PAIR_26_PENALTY=1.222749
PRIMER_LEFT_26_PENALTY=0.042732
PRIMER_RIGHT_26_PENALTY=1.180017
PRIMER_LEFT_26_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_26_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_26=65,20
PRIMER_RIGHT_26=292,20
PRIMER_LEFT_26_TM=59.957
PRIMER_RIGHT_26_TM=61.180
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=2.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=228
PRIMER_PAIR_26_PRODUCT_TM=69.9
PRIMER_PAIR_27_PENALTY=1.249591
PRIMER_LEFT_27_PENALTY=0.042732
PRIMER_RIGHT_27_PENALTY=1.206859
PRIMER_LEFT_27_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_27_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_27=65,20
PRIMER_RIGHT_27=290,19
PRIMER_LEFT_27_TM=59.957
PRIMER_RIGHT_27_TM=60.207
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=57.895
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=2.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=226
PRIMER_PAIR_27_PRODUCT_TM=69.8
PRIMER_PAIR_28_PENALTY=1.249591
PRIMER_LEFT_28_PENALTY=0.042732
PRIMER_RIGHT_28_PENALTY=1.206859
PRIMER_LEFT_28_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_28_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_28=65,20
PRIMER_RIGHT_28=291,19
PRIMER_LEFT_28_TM=59.957
PRIMER_RIGHT_28_TM=60.207
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=57.895
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=2.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=227
PRIMER_PAIR_28_PRODUCT_TM=69.9
PRIMER_PAIR_29_PENALTY=1.313757
PRIMER_LEFT_29_PENALTY=0.133741
PRIMER_RIGHT_29_PENALTY=1.180017
PRIMER_LEFT_29_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_29_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_29=53,20
PRIMER_RIGHT_29=290,20
PRIMER_LEFT_29_TM=60.134
PRIMER_RIGHT_29_TM=61.180
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=4.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=11.2000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=238
PRIMER_PAIR_29_PRODUCT_TM=70.1
PRIMER_PAIR_30_PENALTY=1.313757
PRIMER_LEFT_30_PENALTY=0.133741
PRIMER_RIGHT_30_PENALTY=1.180017
PRIMER_LEFT_30_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_30_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_30=53,20
PRIMER_RIGHT_30=292,20
PRIMER_LEFT_30_TM=60.134
PRIMER_RIGHT_30_TM=61.180
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=4.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=11.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=240
PRIMER_PAIR_30_PRODUCT_TM=70.2
PRIMER_PAIR_31_PENALTY=1.313757
PRIMER_LEFT_31_PENALTY=0.133741
PRIMER_RIGHT_31_PENALTY=1.180017
PRIMER_LEFT_31_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_31_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_31=52,20
PRIMER_RIGHT_31=290,20
PRIMER_LEFT_31_TM=60.134
PRIMER_RIGHT_31_TM=61.180
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=4.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=10.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=239
PRIMER_PAIR_31_PRODUCT_TM=70.3
PRIMER_PAIR_32_PENALTY=1.313757
PRIMER_LEFT_32_PENALTY=0.133741
PRIMER_RIGHT_32_PENALTY=1.180017
PRIMER_LEFT_32_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_32_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_32=52,20
PRIMER_RIGHT_32=292,20
PRIMER_LEFT_32_TM=60.134
PRIMER_RIGHT_32_TM=61.180
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=4.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=10.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=241
PRIMER_PAIR_32_PRODUCT_TM=70.3
PRIMER_PAIR_33_PENALTY=1.340600
PRIMER_LEFT_33_PENALTY=0.133741
PRIMER_RIGHT_33_PENALTY=1.206859
PRIMER_LEFT_33_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_33_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_33=53,20
PRIMER_RIGHT_33=290,19
PRIMER_LEFT_33_TM=60.134
PRIMER_RIGHT_33_TM=60.207
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=57.895
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=4.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=11.2000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=238
PRIMER_PAIR_33_PRODUCT_TM=70.1
PRIMER_PAIR_34_PENALTY=1.340600
PRIMER_LEFT_34_PENALTY=0.133741
PRIMER_RIGHT_34_PENALTY=1.206859
PRIMER_LEFT_34_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_34_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_34=53,20
PRIMER_RIGHT_34=291,19
PRIMER_LEFT_34_TM=60.134
PRIMER_RIGHT_34_TM=60.207
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=57.895
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=4.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=11.2000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=239
PRIMER_PAIR_34_PRODUCT_TM=70.3
PRIMER_PAIR_35_PENALTY=1.340600
PRIMER_LEFT_35_PENALTY=0.133741
PRIMER_RIGHT_35_PENALTY=1.206859
PRIMER_LEFT_35_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_35_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_35=52,20
PRIMER_RIGHT_35=290,19
PRIMER_LEFT_35_TM=60.134
PRIMER_RIGHT_35_TM=60.207
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=57.895
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=4.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=10.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=239
PRIMER_PAIR_35_PRODUCT_TM=70.3
PRIMER_PAIR_36_PENALTY=1.340600
PRIMER_LEFT_36_PENALTY=0.133741
PRIMER_RIGHT_36_PENALTY=1.206859
PRIMER_LEFT_36_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_36_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_36=52,20
PRIMER_RIGHT_36=291,19
PRIMER_LEFT_36_TM=60.134
PRIMER_RIGHT_36_TM=60.207
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=57.895
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=4.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=240
PRIMER_PAIR_36_PRODUCT_TM=70.4
PRIMER_PAIR_37_PENALTY=1.350422
PRIMER_LEFT_37_PENALTY=0.003322
PRIMER_RIGHT_37_PENALTY=1.347100
PRIMER_LEFT_37_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_37_SEQUENCE=TCCTTCCCACAACCAACCT
PRIMER_LEFT_37=54,20
PRIMER_RIGHT_37=289,19
PRIMER_LEFT_37_TM=59.997
PRIMER_RIGHT_37_TM=60.347
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=52.632
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=3.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=236
PRIMER_PAIR_37_PRODUCT_TM=70.0
PRIMER_PAIR_38_PENALTY=1.350422
PRIMER_LEFT_38_PENALTY=0.003322
PRIMER_RIGHT_38_PENALTY=1.347100
PRIMER_LEFT_38_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_38_SEQUENCE=AGGTCCTTCCCACAACCAA
PRIMER_LEFT_38=54,20
PRIMER_RIGHT_38=292,19
PRIMER_LEFT_38_TM=59.997
PRIMER_RIGHT_38_TM=60.347
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=52.632
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=3.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=239
PRIMER_PAIR_38_PRODUCT_TM=70.3
PRIMER_PAIR_39_PENALTY=1.350422
PRIMER_LEFT_39_PENALTY=0.003322
PRIMER_RIGHT_39_PENALTY=1.347100
PRIMER_LEFT_39_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_39_SEQUENCE=AAGGTCCTTCCCACAACCA
PRIMER_LEFT_39=54,20
PRIMER_RIGHT_39=293,19
PRIMER_LEFT_39_TM=59.997
PRIMER_RIGHT_39_TM=60.347
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=52.632
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=7.00
PRIMER_LEFT_39_SELF_END=3.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=240
PRIMER_PAIR_39_PRODUCT_TM=70.2
=
SEQUENCE_ID=06.RHAP34FE6.seq
SEQUENCE_TEMPLATE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
SEQUENCE_INCLUDED_REGION=8,287
SEQUENCE_TARGET=61,68
PRIMER_LEFT_NUM_RETURNED=40
PRIMER_RIGHT_NUM_RETURNED=40
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=40
PRIMER_PAIR_0_PENALTY=2.096487
PRIMER_LEFT_0_PENALTY=1.153194
PRIMER_RIGHT_0_PENALTY=0.943292
PRIMER_LEFT_0_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_0_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_0=9,21
PRIMER_RIGHT_0=148,20
PRIMER_LEFT_0_TM=59.847
PRIMER_RIGHT_0_TM=60.943
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=65.6
PRIMER_PAIR_1_PENALTY=2.623573
PRIMER_LEFT_1_PENALTY=1.153194
PRIMER_RIGHT_1_PENALTY=1.470379
PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=148,19
PRIMER_LEFT_1_TM=59.847
PRIMER_RIGHT_1_TM=59.530
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=140
PRIMER_PAIR_1_PRODUCT_TM=65.6
PRIMER_PAIR_2_PENALTY=2.943089
PRIMER_LEFT_2_PENALTY=1.999797
PRIMER_RIGHT_2_PENALTY=0.943292
PRIMER_LEFT_2_SEQUENCE=CAACCCAAAAATACAGACGG
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_2=9,20
PRIMER_RIGHT_2=148,20
PRIMER_LEFT_2_TM=58.000
PRIMER_RIGHT_2_TM=60.943
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=140
PRIMER_PAIR_2_PRODUCT_TM=65.6
PRIMER_PAIR_3_PENALTY=2.943089
PRIMER_LEFT_3_PENALTY=1.999797
PRIMER_RIGHT_3_PENALTY=0.943292
PRIMER_LEFT_3_SEQUENCE=CCAACCCAAAAATACAGACG
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_3=8,20
PRIMER_RIGHT_3=148,20
PRIMER_LEFT_3_TM=58.000
PRIMER_RIGHT_3_TM=60.943
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=141
PRIMER_PAIR_3_PRODUCT_TM=65.8
PRIMER_PAIR_4_PENALTY=3.406904
PRIMER_LEFT_4_PENALTY=2.463612
PRIMER_RIGHT_4_PENALTY=0.943292
PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=148,20
PRIMER_LEFT_4_TM=61.464
PRIMER_RIGHT_4_TM=60.943
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=141
PRIMER_PAIR_4_PRODUCT_TM=65.8
PRIMER_PAIR_5_PENALTY=3.470176
PRIMER_LEFT_5_PENALTY=1.999797
PRIMER_RIGHT_5_PENALTY=1.470379
PRIMER_LEFT_5_SEQUENCE=CAACCCAAAAATACAGACGG
PRIMER_RIGHT_5_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_5=9,20
PRIMER_RIGHT_5=148,19
PRIMER_LEFT_5_TM=58.000
PRIMER_RIGHT_5_TM=59.530
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=57.895
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=4.00
PRIMER_PAIR_5_PRODUCT_SIZE=140
PRIMER_PAIR_5_PRODUCT_TM=65.6
PRIMER_PAIR_6_PENALTY=3.470176
PRIMER_LEFT_6_PENALTY=1.999797
PRIMER_RIGHT_6_PENALTY=1.470379
PRIMER_LEFT_6_SEQUENCE=CCAACCCAAAAATACAGACG
PRIMER_RIGHT_6_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_6=8,20
PRIMER_RIGHT_6=148,19
PRIMER_LEFT_6_TM=58.000
PRIMER_RIGHT_6_TM=59.530
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=57.895
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=141
PRIMER_PAIR_6_PRODUCT_TM=65.8
PRIMER_PAIR_7_PENALTY=3.876831
PRIMER_LEFT_7_PENALTY=2.933539
PRIMER_RIGHT_7_PENALTY=0.943292
PRIMER_LEFT_7_SEQUENCE=AACCCAAAAATACAGACGGA
PRIMER_RIGHT_7_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_7=10,20
PRIMER_RIGHT_7=148,20
PRIMER_LEFT_7_TM=57.066
PRIMER_RIGHT_7_TM=60.943
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=4.00
PRIMER_PAIR_7_PRODUCT_SIZE=139
PRIMER_PAIR_7_PRODUCT_TM=65.3
PRIMER_PAIR_8_PENALTY=3.933991
PRIMER_LEFT_8_PENALTY=2.463612
PRIMER_RIGHT_8_PENALTY=1.470379
PRIMER_LEFT_8_SEQUENCE=CCAACCCAAAAATACAGACGG
PRIMER_RIGHT_8_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_8=8,21
PRIMER_RIGHT_8=148,19
PRIMER_LEFT_8_TM=61.464
PRIMER_RIGHT_8_TM=59.530
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=57.895
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=4.00
PRIMER_PAIR_8_PRODUCT_SIZE=141
PRIMER_PAIR_8_PRODUCT_TM=65.8
PRIMER_PAIR_9_PENALTY=4.403918
PRIMER_LEFT_9_PENALTY=2.933539
PRIMER_RIGHT_9_PENALTY=1.470379
PRIMER_LEFT_9_SEQUENCE=AACCCAAAAATACAGACGGA
PRIMER_RIGHT_9_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_9=10,20
PRIMER_RIGHT_9=148,19
PRIMER_LEFT_9_TM=57.066
PRIMER_RIGHT_9_TM=59.530
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=57.895
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=139
PRIMER_PAIR_9_PRODUCT_TM=65.3
PRIMER_PAIR_10_PENALTY=4.451682
PRIMER_LEFT_10_PENALTY=4.436430
PRIMER_RIGHT_10_PENALTY=0.015253
PRIMER_LEFT_10_SEQUENCE=CACAAACAGAGACACAGACACAGA
PRIMER_RIGHT_10_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
PRIMER_LEFT_10=36,24
PRIMER_RIGHT_10=210,20
PRIMER_LEFT_10_TM=60.436
PRIMER_RIGHT_10_TM=60.015
PRIMER_LEFT_10_GC_PERCENT=45.833
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=1.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=4.00
PRIMER_PAIR_10_PRODUCT_SIZE=175
PRIMER_PAIR_10_PRODUCT_TM=68.0
PRIMER_PAIR_11_PENALTY=4.497659
PRIMER_LEFT_11_PENALTY=4.436430
PRIMER_RIGHT_11_PENALTY=0.061230
PRIMER_LEFT_11_SEQUENCE=CACAAACAGAGACACAGACACAGA
PRIMER_RIGHT_11_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_11=36,24
PRIMER_RIGHT_11=203,20
PRIMER_LEFT_11_TM=60.436
PRIMER_RIGHT_11_TM=60.061
PRIMER_LEFT_11_GC_PERCENT=45.833
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=1.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=5.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=168
PRIMER_PAIR_11_PRODUCT_TM=67.6
PRIMER_PAIR_12_PENALTY=4.529170
PRIMER_LEFT_12_PENALTY=4.513917
PRIMER_RIGHT_12_PENALTY=0.015253
PRIMER_LEFT_12_SEQUENCE=CACAAACAGAGACACAGACACAG
PRIMER_RIGHT_12_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
PRIMER_LEFT_12=36,23
PRIMER_RIGHT_12=210,20
PRIMER_LEFT_12_TM=58.486
PRIMER_RIGHT_12_TM=60.015
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=1.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=175
PRIMER_PAIR_12_PRODUCT_TM=68.0
PRIMER_PAIR_13_PENALTY=4.575147
PRIMER_LEFT_13_PENALTY=4.513917
PRIMER_RIGHT_13_PENALTY=0.061230
PRIMER_LEFT_13_SEQUENCE=CACAAACAGAGACACAGACACAG
PRIMER_RIGHT_13_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_13=36,23
PRIMER_RIGHT_13=203,20
PRIMER_LEFT_13_TM=58.486
PRIMER_RIGHT_13_TM=60.061
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=1.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=5.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=168
PRIMER_PAIR_13_PRODUCT_TM=67.6
PRIMER_PAIR_14_PENALTY=4.670466
PRIMER_LEFT_14_PENALTY=4.609236
PRIMER_RIGHT_14_PENALTY=0.061230
PRIMER_LEFT_14_SEQUENCE=ACACAAACAGAGACACAGACACAG
PRIMER_RIGHT_14_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_14=35,24
PRIMER_RIGHT_14=203,20
PRIMER_LEFT_14_TM=59.391
PRIMER_RIGHT_14_TM=60.061
PRIMER_LEFT_14_GC_PERCENT=45.833
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=1.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=169
PRIMER_PAIR_14_PRODUCT_TM=67.5
PRIMER_PAIR_15_PENALTY=4.684266
PRIMER_LEFT_15_PENALTY=4.669014
PRIMER_RIGHT_15_PENALTY=0.015253
PRIMER_LEFT_15_SEQUENCE=CACAAACAGAGACACAGACACA
PRIMER_RIGHT_15_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
PRIMER_LEFT_15=36,22
PRIMER_RIGHT_15=210,20
PRIMER_LEFT_15_TM=57.331
PRIMER_RIGHT_15_TM=60.015
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=1.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=175
PRIMER_PAIR_15_PRODUCT_TM=68.0
PRIMER_PAIR_16_PENALTY=4.730243
PRIMER_LEFT_16_PENALTY=4.669014
PRIMER_RIGHT_16_PENALTY=0.061230
PRIMER_LEFT_16_SEQUENCE=CACAAACAGAGACACAGACACA
PRIMER_RIGHT_16_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_16=36,22
PRIMER_RIGHT_16=203,20
PRIMER_LEFT_16_TM=57.331
PRIMER_RIGHT_16_TM=60.061
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=1.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=5.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=168
PRIMER_PAIR_16_PRODUCT_TM=67.6
PRIMER_PAIR_17_PENALTY=4.730243
PRIMER_LEFT_17_PENALTY=4.669014
PRIMER_RIGHT_17_PENALTY=0.061230
PRIMER_LEFT_17_SEQUENCE=CACACAAACAGAGACACAGACA
PRIMER_RIGHT_17_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_17=34,22
PRIMER_RIGHT_17=203,20
PRIMER_LEFT_17_TM=57.331
PRIMER_RIGHT_17_TM=60.061
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=1.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=170
PRIMER_PAIR_17_PRODUCT_TM=67.7
PRIMER_PAIR_18_PENALTY=4.730243
PRIMER_LEFT_18_PENALTY=4.669014
PRIMER_RIGHT_18_PENALTY=0.061230
PRIMER_LEFT_18_SEQUENCE=CACACACAAACAGAGACACAGA
PRIMER_RIGHT_18_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_18=32,22
PRIMER_RIGHT_18=203,20
PRIMER_LEFT_18_TM=57.331
PRIMER_RIGHT_18_TM=60.061
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=1.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=172
PRIMER_PAIR_18_PRODUCT_TM=67.9
PRIMER_PAIR_19_PENALTY=4.730755
PRIMER_LEFT_19_PENALTY=4.669525
PRIMER_RIGHT_19_PENALTY=0.061230
PRIMER_LEFT_19_SEQUENCE=ACACAAACAGAGACACAGACACA
PRIMER_RIGHT_19_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_19=35,23
PRIMER_RIGHT_19=203,20
PRIMER_LEFT_19_TM=58.330
PRIMER_RIGHT_19_TM=60.061
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=1.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=169
PRIMER_PAIR_19_PRODUCT_TM=67.5
PRIMER_PAIR_20_PENALTY=4.730755
PRIMER_LEFT_20_PENALTY=4.669525
PRIMER_RIGHT_20_PENALTY=0.061230
PRIMER_LEFT_20_SEQUENCE=CACACAAACAGAGACACAGACAC
PRIMER_RIGHT_20_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_20=34,23
PRIMER_RIGHT_20=203,20
PRIMER_LEFT_20_TM=58.330
PRIMER_RIGHT_20_TM=60.061
PRIMER_LEFT_20_GC_PERCENT=47.826
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=1.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.1000
PRIMER_RIGHT_20_END_STABILITY=5.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=170
PRIMER_PAIR_20_PRODUCT_TM=67.7
PRIMER_PAIR_21_PENALTY=4.730755
PRIMER_LEFT_21_PENALTY=4.669525
PRIMER_RIGHT_21_PENALTY=0.061230
PRIMER_LEFT_21_SEQUENCE=ACACACAAACAGAGACACAGACA
PRIMER_RIGHT_21_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_21=33,23
PRIMER_RIGHT_21=203,20
PRIMER_LEFT_21_TM=58.330
PRIMER_RIGHT_21_TM=60.061
PRIMER_LEFT_21_GC_PERCENT=43.478
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=1.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=5.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=171
PRIMER_PAIR_21_PRODUCT_TM=67.7
PRIMER_PAIR_22_PENALTY=4.730755
PRIMER_LEFT_22_PENALTY=4.669525
PRIMER_RIGHT_22_PENALTY=0.061230
PRIMER_LEFT_22_SEQUENCE=CACACACAAACAGAGACACAGAC
PRIMER_RIGHT_22_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_22=32,23
PRIMER_RIGHT_22=203,20
PRIMER_LEFT_22_TM=58.330
PRIMER_RIGHT_22_TM=60.061
PRIMER_LEFT_22_GC_PERCENT=47.826
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=1.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=5.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=172
PRIMER_PAIR_22_PRODUCT_TM=67.9
PRIMER_PAIR_23_PENALTY=4.730755
PRIMER_LEFT_23_PENALTY=4.669525
PRIMER_RIGHT_23_PENALTY=0.061230
PRIMER_LEFT_23_SEQUENCE=ACACACACAAACAGAGACACAGA
PRIMER_RIGHT_23_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_23=31,23
PRIMER_RIGHT_23=203,20
PRIMER_LEFT_23_TM=58.330
PRIMER_RIGHT_23_TM=60.061
PRIMER_LEFT_23_GC_PERCENT=43.478
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=1.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=5.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=173
PRIMER_PAIR_23_PRODUCT_TM=67.8
PRIMER_PAIR_24_PENALTY=4.812339
PRIMER_LEFT_24_PENALTY=4.751110
PRIMER_RIGHT_24_PENALTY=0.061230
PRIMER_LEFT_24_SEQUENCE=ACACACAAACAGAGACACAGACAC
PRIMER_RIGHT_24_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_24=33,24
PRIMER_RIGHT_24=203,20
PRIMER_LEFT_24_TM=59.249
PRIMER_RIGHT_24_TM=60.061
PRIMER_LEFT_24_GC_PERCENT=45.833
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=1.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.1000
PRIMER_RIGHT_24_END_STABILITY=5.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=171
PRIMER_PAIR_24_PRODUCT_TM=67.7
PRIMER_PAIR_25_PENALTY=4.812339
PRIMER_LEFT_25_PENALTY=4.751110
PRIMER_RIGHT_25_PENALTY=0.061230
PRIMER_LEFT_25_SEQUENCE=ACACACACAAACAGAGACACAGAC
PRIMER_RIGHT_25_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_25=31,24
PRIMER_RIGHT_25=203,20
PRIMER_LEFT_25_TM=59.249
PRIMER_RIGHT_25_TM=60.061
PRIMER_LEFT_25_GC_PERCENT=45.833
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=1.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=5.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=173
PRIMER_PAIR_25_PRODUCT_TM=67.8
PRIMER_PAIR_26_PENALTY=4.907661
PRIMER_LEFT_26_PENALTY=4.436430
PRIMER_RIGHT_26_PENALTY=0.471231
PRIMER_LEFT_26_SEQUENCE=CACAAACAGAGACACAGACACAGA
PRIMER_RIGHT_26_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_26=36,24
PRIMER_RIGHT_26=205,20
PRIMER_LEFT_26_TM=60.436
PRIMER_RIGHT_26_TM=60.471
PRIMER_LEFT_26_GC_PERCENT=45.833
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=1.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=2.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=7.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=170
PRIMER_PAIR_26_PRODUCT_TM=67.7
PRIMER_PAIR_27_PENALTY=4.985149
PRIMER_LEFT_27_PENALTY=4.513917
PRIMER_RIGHT_27_PENALTY=0.471231
PRIMER_LEFT_27_SEQUENCE=CACAAACAGAGACACAGACACAG
PRIMER_RIGHT_27_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_27=36,23
PRIMER_RIGHT_27=205,20
PRIMER_LEFT_27_TM=58.486
PRIMER_RIGHT_27_TM=60.471
PRIMER_LEFT_27_GC_PERCENT=47.826
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=1.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=2.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=170
PRIMER_PAIR_27_PRODUCT_TM=67.7
PRIMER_PAIR_28_PENALTY=5.080467
PRIMER_LEFT_28_PENALTY=4.609236
PRIMER_RIGHT_28_PENALTY=0.471231
PRIMER_LEFT_28_SEQUENCE=ACACAAACAGAGACACAGACACAG
PRIMER_RIGHT_28_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_28=35,24
PRIMER_RIGHT_28=205,20
PRIMER_LEFT_28_TM=59.391
PRIMER_RIGHT_28_TM=60.471
PRIMER_LEFT_28_GC_PERCENT=45.833
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=1.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=2.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=171
PRIMER_PAIR_28_PRODUCT_TM=67.7
PRIMER_PAIR_29_PENALTY=5.095753
PRIMER_LEFT_29_PENALTY=1.153194
PRIMER_RIGHT_29_PENALTY=3.942558
PRIMER_LEFT_29_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_29_SEQUENCE=CCTGTGTTACCCGTCTCAA
PRIMER_LEFT_29=9,21
PRIMER_RIGHT_29=147,19
PRIMER_LEFT_29_TM=59.847
PRIMER_RIGHT_29_TM=57.057
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=52.632
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=4.00
PRIMER_PAIR_29_PRODUCT_SIZE=139
PRIMER_PAIR_29_PRODUCT_TM=65.3
PRIMER_PAIR_30_PENALTY=5.140245
PRIMER_LEFT_30_PENALTY=4.669014
PRIMER_RIGHT_30_PENALTY=0.471231
PRIMER_LEFT_30_SEQUENCE=CACAAACAGAGACACAGACACA
PRIMER_RIGHT_30_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_30=36,22
PRIMER_RIGHT_30=205,20
PRIMER_LEFT_30_TM=57.331
PRIMER_RIGHT_30_TM=60.471
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=1.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=2.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=170
PRIMER_PAIR_30_PRODUCT_TM=67.7
PRIMER_PAIR_31_PENALTY=5.140245
PRIMER_LEFT_31_PENALTY=4.669014
PRIMER_RIGHT_31_PENALTY=0.471231
PRIMER_LEFT_31_SEQUENCE=CACACAAACAGAGACACAGACA
PRIMER_RIGHT_31_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_31=34,22
PRIMER_RIGHT_31=205,20
PRIMER_LEFT_31_TM=57.331
PRIMER_RIGHT_31_TM=60.471
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=1.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=2.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.1000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=172
PRIMER_PAIR_31_PRODUCT_TM=67.9
PRIMER_PAIR_32_PENALTY=5.140245
PRIMER_LEFT_32_PENALTY=4.669014
PRIMER_RIGHT_32_PENALTY=0.471231
PRIMER_LEFT_32_SEQUENCE=CACACACAAACAGAGACACAGA
PRIMER_RIGHT_32_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_32=32,22
PRIMER_RIGHT_32=205,20
PRIMER_LEFT_32_TM=57.331
PRIMER_RIGHT_32_TM=60.471
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=1.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=2.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=174
PRIMER_PAIR_32_PRODUCT_TM=68.0
PRIMER_PAIR_33_PENALTY=5.140757
PRIMER_LEFT_33_PENALTY=4.669525
PRIMER_RIGHT_33_PENALTY=0.471231
PRIMER_LEFT_33_SEQUENCE=ACACAAACAGAGACACAGACACA
PRIMER_RIGHT_33_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_33=35,23
PRIMER_RIGHT_33=205,20
PRIMER_LEFT_33_TM=58.330
PRIMER_RIGHT_33_TM=60.471
PRIMER_LEFT_33_GC_PERCENT=43.478
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=1.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=2.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=7.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=171
PRIMER_PAIR_33_PRODUCT_TM=67.7
PRIMER_PAIR_34_PENALTY=5.140757
PRIMER_LEFT_34_PENALTY=4.669525
PRIMER_RIGHT_34_PENALTY=0.471231
PRIMER_LEFT_34_SEQUENCE=CACACAAACAGAGACACAGACAC
PRIMER_RIGHT_34_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_34=34,23
PRIMER_RIGHT_34=205,20
PRIMER_LEFT_34_TM=58.330
PRIMER_RIGHT_34_TM=60.471
PRIMER_LEFT_34_GC_PERCENT=47.826
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=1.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=2.00
PRIMER_LEFT_34_END_STABILITY=6.1000
PRIMER_RIGHT_34_END_STABILITY=7.1000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=172
PRIMER_PAIR_34_PRODUCT_TM=67.9
PRIMER_PAIR_35_PENALTY=5.140757
PRIMER_LEFT_35_PENALTY=4.669525
PRIMER_RIGHT_35_PENALTY=0.471231
PRIMER_LEFT_35_SEQUENCE=ACACACAAACAGAGACACAGACA
PRIMER_RIGHT_35_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_35=33,23
PRIMER_RIGHT_35=205,20
PRIMER_LEFT_35_TM=58.330
PRIMER_RIGHT_35_TM=60.471
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=1.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=2.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=173
PRIMER_PAIR_35_PRODUCT_TM=67.8
PRIMER_PAIR_36_PENALTY=5.140757
PRIMER_LEFT_36_PENALTY=4.669525
PRIMER_RIGHT_36_PENALTY=0.471231
PRIMER_LEFT_36_SEQUENCE=CACACACAAACAGAGACACAGAC
PRIMER_RIGHT_36_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_36=32,23
PRIMER_RIGHT_36=205,20
PRIMER_LEFT_36_TM=58.330
PRIMER_RIGHT_36_TM=60.471
PRIMER_LEFT_36_GC_PERCENT=47.826
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=1.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=2.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=7.1000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=174
PRIMER_PAIR_36_PRODUCT_TM=68.0
PRIMER_PAIR_37_PENALTY=5.140757
PRIMER_LEFT_37_PENALTY=4.669525
PRIMER_RIGHT_37_PENALTY=0.471231
PRIMER_LEFT_37_SEQUENCE=ACACACACAAACAGAGACACAGA
PRIMER_RIGHT_37_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_37=31,23
PRIMER_RIGHT_37=205,20
PRIMER_LEFT_37_TM=58.330
PRIMER_RIGHT_37_TM=60.471
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=1.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=2.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=7.1000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=175
PRIMER_PAIR_37_PRODUCT_TM=68.0
PRIMER_PAIR_38_PENALTY=5.222341
PRIMER_LEFT_38_PENALTY=4.751110
PRIMER_RIGHT_38_PENALTY=0.471231
PRIMER_LEFT_38_SEQUENCE=ACACACAAACAGAGACACAGACAC
PRIMER_RIGHT_38_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_38=33,24
PRIMER_RIGHT_38=205,20
PRIMER_LEFT_38_TM=59.249
PRIMER_RIGHT_38_TM=60.471
PRIMER_LEFT_38_GC_PERCENT=45.833
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=1.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=2.00
PRIMER_LEFT_38_END_STABILITY=6.1000
PRIMER_RIGHT_38_END_STABILITY=7.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=173
PRIMER_PAIR_38_PRODUCT_TM=67.8
PRIMER_PAIR_39_PENALTY=5.222341
PRIMER_LEFT_39_PENALTY=4.751110
PRIMER_RIGHT_39_PENALTY=0.471231
PRIMER_LEFT_39_SEQUENCE=ACACACACAAACAGAGACACAGAC
PRIMER_RIGHT_39_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_39=31,24
PRIMER_RIGHT_39=205,20
PRIMER_LEFT_39_TM=59.249
PRIMER_RIGHT_39_TM=60.471
PRIMER_LEFT_39_GC_PERCENT=45.833
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=1.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=2.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.1000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=175
PRIMER_PAIR_39_PRODUCT_TM=68.0
=
SEQUENCE_ID=07.RHAP34FE7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
SEQUENCE_INCLUDED_REGION=18,283
SEQUENCE_TARGET=115,29
SEQUENCE_TARGET=145,20
SEQUENCE_TARGET=166,24
PRIMER_LEFT_NUM_RETURNED=40
PRIMER_RIGHT_NUM_RETURNED=40
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=40
PRIMER_PAIR_0_PENALTY=0.484594
PRIMER_LEFT_0_PENALTY=0.151542
PRIMER_RIGHT_0_PENALTY=0.333053
PRIMER_LEFT_0_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_0_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=238,20
PRIMER_LEFT_0_TM=60.152
PRIMER_RIGHT_0_TM=60.333
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=163
PRIMER_PAIR_0_PRODUCT_TM=67.3
PRIMER_PAIR_1_PENALTY=0.681190
PRIMER_LEFT_1_PENALTY=0.151542
PRIMER_RIGHT_1_PENALTY=0.529649
PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_1=76,20
PRIMER_RIGHT_1=235,20
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=59.470
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=160
PRIMER_PAIR_1_PRODUCT_TM=67.2
PRIMER_PAIR_2_PENALTY=0.789733
PRIMER_LEFT_2_PENALTY=0.151542
PRIMER_RIGHT_2_PENALTY=0.638191
PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_2=76,20
PRIMER_RIGHT_2=225,20
PRIMER_LEFT_2_TM=60.152
PRIMER_RIGHT_2_TM=60.638
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_2_PRODUCT_TM=66.0
PRIMER_PAIR_3_PENALTY=0.828489
PRIMER_LEFT_3_PENALTY=0.151542
PRIMER_RIGHT_3_PENALTY=0.676947
PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_3=76,20
PRIMER_RIGHT_3=237,20
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=59.323
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=162
PRIMER_PAIR_3_PRODUCT_TM=67.3
PRIMER_PAIR_4_PENALTY=0.837797
PRIMER_LEFT_4_PENALTY=0.151542
PRIMER_RIGHT_4_PENALTY=0.686256
PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_4_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_4=76,20
PRIMER_RIGHT_4=232,20
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=59.314
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=157
PRIMER_PAIR_4_PRODUCT_TM=66.8
PRIMER_PAIR_5_PENALTY=0.837797
PRIMER_LEFT_5_PENALTY=0.151542
PRIMER_RIGHT_5_PENALTY=0.686256
PRIMER_LEFT_5_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_5_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_5=76,20
PRIMER_RIGHT_5=234,20
PRIMER_LEFT_5_TM=60.152
PRIMER_RIGHT_5_TM=59.314
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=159
PRIMER_PAIR_5_PRODUCT_TM=67.0
PRIMER_PAIR_6_PENALTY=0.918041
PRIMER_LEFT_6_PENALTY=0.151542
PRIMER_RIGHT_6_PENALTY=0.766499
PRIMER_LEFT_6_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_6_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_6=76,20
PRIMER_RIGHT_6=244,20
PRIMER_LEFT_6_TM=60.152
PRIMER_RIGHT_6_TM=60.766
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=6.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=169
PRIMER_PAIR_6_PRODUCT_TM=67.5
PRIMER_PAIR_7_PENALTY=0.956229
PRIMER_LEFT_7_PENALTY=0.151542
PRIMER_RIGHT_7_PENALTY=0.804687
PRIMER_LEFT_7_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_7_SEQUENCE=ATGTGCACGCATGTAGAGGT
PRIMER_LEFT_7=76,20
PRIMER_RIGHT_7=239,20
PRIMER_LEFT_7_TM=60.152
PRIMER_RIGHT_7_TM=59.195
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=164
PRIMER_PAIR_7_PRODUCT_TM=67.2
PRIMER_PAIR_8_PENALTY=1.016725
PRIMER_LEFT_8_PENALTY=0.683672
PRIMER_RIGHT_8_PENALTY=0.333053
PRIMER_LEFT_8_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_8_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_8=88,20
PRIMER_RIGHT_8=238,20
PRIMER_LEFT_8_TM=59.316
PRIMER_RIGHT_8_TM=60.333
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=5.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=151
PRIMER_PAIR_8_PRODUCT_TM=66.5
PRIMER_PAIR_9_PENALTY=1.108771
PRIMER_LEFT_9_PENALTY=0.775718
PRIMER_RIGHT_9_PENALTY=0.333053
PRIMER_LEFT_9_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_9_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_9=87,20
PRIMER_RIGHT_9=238,20
PRIMER_LEFT_9_TM=60.776
PRIMER_RIGHT_9_TM=60.333
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=152
PRIMER_PAIR_9_PRODUCT_TM=66.5
PRIMER_PAIR_10_PENALTY=1.108771
PRIMER_LEFT_10_PENALTY=0.775718
PRIMER_RIGHT_10_PENALTY=0.333053
PRIMER_LEFT_10_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_10_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_10=86,20
PRIMER_RIGHT_10=238,20
PRIMER_LEFT_10_TM=60.776
PRIMER_RIGHT_10_TM=60.333
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=153
PRIMER_PAIR_10_PRODUCT_TM=66.7
PRIMER_PAIR_11_PENALTY=1.213321
PRIMER_LEFT_11_PENALTY=0.683672
PRIMER_RIGHT_11_PENALTY=0.529649
PRIMER_LEFT_11_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_11_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_11=88,20
PRIMER_RIGHT_11=235,20
PRIMER_LEFT_11_TM=59.316
PRIMER_RIGHT_11_TM=59.470
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=5.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=148
PRIMER_PAIR_11_PRODUCT_TM=66.4
PRIMER_PAIR_12_PENALTY=1.277825
PRIMER_LEFT_12_PENALTY=0.151542
PRIMER_RIGHT_12_PENALTY=1.126283
PRIMER_LEFT_12_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_12_SEQUENCE=GCATGTAGAGGTTCAGGTGGA
PRIMER_LEFT_12=76,20
PRIMER_RIGHT_12=231,21
PRIMER_LEFT_12_TM=60.152
PRIMER_RIGHT_12_TM=60.126
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=156
PRIMER_PAIR_12_PRODUCT_TM=66.6
PRIMER_PAIR_13_PENALTY=1.305367
PRIMER_LEFT_13_PENALTY=0.775718
PRIMER_RIGHT_13_PENALTY=0.529649
PRIMER_LEFT_13_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_13_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_13=87,20
PRIMER_RIGHT_13=235,20
PRIMER_LEFT_13_TM=60.776
PRIMER_RIGHT_13_TM=59.470
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=149
PRIMER_PAIR_13_PRODUCT_TM=66.3
PRIMER_PAIR_14_PENALTY=1.305367
PRIMER_LEFT_14_PENALTY=0.775718
PRIMER_RIGHT_14_PENALTY=0.529649
PRIMER_LEFT_14_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_14_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_14=86,20
PRIMER_RIGHT_14=235,20
PRIMER_LEFT_14_TM=60.776
PRIMER_RIGHT_14_TM=59.470
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=150
PRIMER_PAIR_14_PRODUCT_TM=66.6
PRIMER_PAIR_15_PENALTY=1.321863
PRIMER_LEFT_15_PENALTY=0.683672
PRIMER_RIGHT_15_PENALTY=0.638191
PRIMER_LEFT_15_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_15_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_15=88,20
PRIMER_RIGHT_15=225,20
PRIMER_LEFT_15_TM=59.316
PRIMER_RIGHT_15_TM=60.638
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=60.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=5.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=138
PRIMER_PAIR_15_PRODUCT_TM=65.1
PRIMER_PAIR_16_PENALTY=1.337484
PRIMER_LEFT_16_PENALTY=0.151542
PRIMER_RIGHT_16_PENALTY=1.185942
PRIMER_LEFT_16_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_16_SEQUENCE=ACGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_16=76,20
PRIMER_RIGHT_16=233,21
PRIMER_LEFT_16_TM=60.152
PRIMER_RIGHT_16_TM=60.186
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=158
PRIMER_PAIR_16_PRODUCT_TM=66.7
PRIMER_PAIR_17_PENALTY=1.337484
PRIMER_LEFT_17_PENALTY=0.151542
PRIMER_RIGHT_17_PENALTY=1.185942
PRIMER_LEFT_17_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_17_SEQUENCE=CACGCATGTAGAGGTTCAGGT
PRIMER_LEFT_17=76,20
PRIMER_RIGHT_17=234,21
PRIMER_LEFT_17_TM=60.152
PRIMER_RIGHT_17_TM=60.186
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=159
PRIMER_PAIR_17_PRODUCT_TM=67.0
PRIMER_PAIR_18_PENALTY=1.348445
PRIMER_LEFT_18_PENALTY=0.151542
PRIMER_RIGHT_18_PENALTY=1.196903
PRIMER_LEFT_18_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_18_SEQUENCE=AACATGTGCACGCATGTAGAG
PRIMER_LEFT_18=76,20
PRIMER_RIGHT_18=242,21
PRIMER_LEFT_18_TM=60.152
PRIMER_RIGHT_18_TM=59.803
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=5.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=167
PRIMER_PAIR_18_PRODUCT_TM=67.4
PRIMER_PAIR_19_PENALTY=1.355841
PRIMER_LEFT_19_PENALTY=1.022788
PRIMER_RIGHT_19_PENALTY=0.333053
PRIMER_LEFT_19_SEQUENCE=TTTGGGTACAAACTGACAGGC
PRIMER_RIGHT_19_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_19=74,21
PRIMER_RIGHT_19=238,20
PRIMER_LEFT_19_TM=60.023
PRIMER_RIGHT_19_TM=60.333
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=4.00
PRIMER_PAIR_19_PRODUCT_SIZE=165
PRIMER_PAIR_19_PRODUCT_TM=67.2
PRIMER_PAIR_20_PENALTY=1.360619
PRIMER_LEFT_20_PENALTY=0.683672
PRIMER_RIGHT_20_PENALTY=0.676947
PRIMER_LEFT_20_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_20_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_20=88,20
PRIMER_RIGHT_20=237,20
PRIMER_LEFT_20_TM=59.316
PRIMER_RIGHT_20_TM=59.323
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=5.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=150
PRIMER_PAIR_20_PRODUCT_TM=66.6
PRIMER_PAIR_21_PENALTY=1.369928
PRIMER_LEFT_21_PENALTY=0.683672
PRIMER_RIGHT_21_PENALTY=0.686256
PRIMER_LEFT_21_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_21_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_21=88,20
PRIMER_RIGHT_21=232,20
PRIMER_LEFT_21_TM=59.316
PRIMER_RIGHT_21_TM=59.314
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=5.00
PRIMER_RIGHT_21_SELF_END=2.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=145
PRIMER_PAIR_21_PRODUCT_TM=65.9
PRIMER_PAIR_22_PENALTY=1.369928
PRIMER_LEFT_22_PENALTY=0.683672
PRIMER_RIGHT_22_PENALTY=0.686256
PRIMER_LEFT_22_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_22_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_22=88,20
PRIMER_RIGHT_22=234,20
PRIMER_LEFT_22_TM=59.316
PRIMER_RIGHT_22_TM=59.314
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=5.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=147
PRIMER_PAIR_22_PRODUCT_TM=66.1
PRIMER_PAIR_23_PENALTY=1.413909
PRIMER_LEFT_23_PENALTY=0.775718
PRIMER_RIGHT_23_PENALTY=0.638191
PRIMER_LEFT_23_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_23_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_23=87,20
PRIMER_RIGHT_23=225,20
PRIMER_LEFT_23_TM=60.776
PRIMER_RIGHT_23_TM=60.638
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=139
PRIMER_PAIR_23_PRODUCT_TM=65.0
PRIMER_PAIR_24_PENALTY=1.413909
PRIMER_LEFT_24_PENALTY=0.775718
PRIMER_RIGHT_24_PENALTY=0.638191
PRIMER_LEFT_24_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_24_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_24=86,20
PRIMER_RIGHT_24=225,20
PRIMER_LEFT_24_TM=60.776
PRIMER_RIGHT_24_TM=60.638
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=60.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=140
PRIMER_PAIR_24_PRODUCT_TM=65.3
PRIMER_PAIR_25_PENALTY=1.417827
PRIMER_LEFT_25_PENALTY=0.151542
PRIMER_RIGHT_25_PENALTY=1.266285
PRIMER_LEFT_25_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_25_SEQUENCE=AACATGTGCACGCATGTAGA
PRIMER_LEFT_25=76,20
PRIMER_RIGHT_25=242,20
PRIMER_LEFT_25_TM=60.152
PRIMER_RIGHT_25_TM=58.734
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=8.00
PRIMER_LEFT_25_SELF_END=3.00
PRIMER_RIGHT_25_SELF_END=4.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=5.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=167
PRIMER_PAIR_25_PRODUCT_TM=67.4
PRIMER_PAIR_26_PENALTY=1.417827
PRIMER_LEFT_26_PENALTY=0.151542
PRIMER_RIGHT_26_PENALTY=1.266285
PRIMER_LEFT_26_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_26_SEQUENCE=GAACATGTGCACGCATGTAG
PRIMER_LEFT_26=76,20
PRIMER_RIGHT_26=243,20
PRIMER_LEFT_26_TM=60.152
PRIMER_RIGHT_26_TM=58.734
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=8.00
PRIMER_LEFT_26_SELF_END=3.00
PRIMER_RIGHT_26_SELF_END=4.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=5.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=168
PRIMER_PAIR_26_PRODUCT_TM=67.6
PRIMER_PAIR_27_PENALTY=1.450171
PRIMER_LEFT_27_PENALTY=0.683672
PRIMER_RIGHT_27_PENALTY=0.766499
PRIMER_LEFT_27_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_27_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_27=88,20
PRIMER_RIGHT_27=244,20
PRIMER_LEFT_27_TM=59.316
PRIMER_RIGHT_27_TM=60.766
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=8.00
PRIMER_LEFT_27_SELF_END=5.00
PRIMER_RIGHT_27_SELF_END=6.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=5.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=157
PRIMER_PAIR_27_PRODUCT_TM=66.8
PRIMER_PAIR_28_PENALTY=1.452665
PRIMER_LEFT_28_PENALTY=0.775718
PRIMER_RIGHT_28_PENALTY=0.676947
PRIMER_LEFT_28_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_28_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_28=87,20
PRIMER_RIGHT_28=237,20
PRIMER_LEFT_28_TM=60.776
PRIMER_RIGHT_28_TM=59.323
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=3.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=151
PRIMER_PAIR_28_PRODUCT_TM=66.5
PRIMER_PAIR_29_PENALTY=1.452665
PRIMER_LEFT_29_PENALTY=0.775718
PRIMER_RIGHT_29_PENALTY=0.676947
PRIMER_LEFT_29_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_29_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_29=86,20
PRIMER_RIGHT_29=237,20
PRIMER_LEFT_29_TM=60.776
PRIMER_RIGHT_29_TM=59.323
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=2.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=152
PRIMER_PAIR_29_PRODUCT_TM=66.7
PRIMER_PAIR_30_PENALTY=1.461974
PRIMER_LEFT_30_PENALTY=0.775718
PRIMER_RIGHT_30_PENALTY=0.686256
PRIMER_LEFT_30_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_30_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_30=87,20
PRIMER_RIGHT_30=232,20
PRIMER_LEFT_30_TM=60.776
PRIMER_RIGHT_30_TM=59.314
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=3.00
PRIMER_RIGHT_30_SELF_END=2.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=146
PRIMER_PAIR_30_PRODUCT_TM=65.9
PRIMER_PAIR_31_PENALTY=1.461974
PRIMER_LEFT_31_PENALTY=0.775718
PRIMER_RIGHT_31_PENALTY=0.686256
PRIMER_LEFT_31_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_31_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_31=87,20
PRIMER_RIGHT_31=234,20
PRIMER_LEFT_31_TM=60.776
PRIMER_RIGHT_31_TM=59.314
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=3.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=148
PRIMER_PAIR_31_PRODUCT_TM=66.1
PRIMER_PAIR_32_PENALTY=1.461974
PRIMER_LEFT_32_PENALTY=0.775718
PRIMER_RIGHT_32_PENALTY=0.686256
PRIMER_LEFT_32_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_32_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_32=86,20
PRIMER_RIGHT_32=232,20
PRIMER_LEFT_32_TM=60.776
PRIMER_RIGHT_32_TM=59.314
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=2.00
PRIMER_RIGHT_32_SELF_END=2.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=147
PRIMER_PAIR_32_PRODUCT_TM=66.1
PRIMER_PAIR_33_PENALTY=1.461974
PRIMER_LEFT_33_PENALTY=0.775718
PRIMER_RIGHT_33_PENALTY=0.686256
PRIMER_LEFT_33_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_33_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_33=86,20
PRIMER_RIGHT_33=234,20
PRIMER_LEFT_33_TM=60.776
PRIMER_RIGHT_33_TM=59.314
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=2.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=149
PRIMER_PAIR_33_PRODUCT_TM=66.3
PRIMER_PAIR_34_PENALTY=1.469743
PRIMER_LEFT_34_PENALTY=0.151542
PRIMER_RIGHT_34_PENALTY=1.318201
PRIMER_LEFT_34_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_34_SEQUENCE=CATGTGCACGCATGTAGAGG
PRIMER_LEFT_34=76,20
PRIMER_RIGHT_34=240,20
PRIMER_LEFT_34_TM=60.152
PRIMER_RIGHT_34_TM=61.318
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=3.00
PRIMER_RIGHT_34_SELF_END=4.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=165
PRIMER_PAIR_34_PRODUCT_TM=67.4
PRIMER_PAIR_35_PENALTY=1.488359
PRIMER_LEFT_35_PENALTY=0.683672
PRIMER_RIGHT_35_PENALTY=0.804687
PRIMER_LEFT_35_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_35_SEQUENCE=ATGTGCACGCATGTAGAGGT
PRIMER_LEFT_35=88,20
PRIMER_RIGHT_35=239,20
PRIMER_LEFT_35_TM=59.316
PRIMER_RIGHT_35_TM=59.195
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=5.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=152
PRIMER_PAIR_35_PRODUCT_TM=66.5
PRIMER_PAIR_36_PENALTY=1.529357
PRIMER_LEFT_36_PENALTY=0.151542
PRIMER_RIGHT_36_PENALTY=1.377815
PRIMER_LEFT_36_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_36_SEQUENCE=GAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_36=76,20
PRIMER_RIGHT_36=224,19
PRIMER_LEFT_36_TM=60.152
PRIMER_RIGHT_36_TM=59.622
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=63.158
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=3.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=149
PRIMER_PAIR_36_PRODUCT_TM=66.1
PRIMER_PAIR_37_PENALTY=1.542217
PRIMER_LEFT_37_PENALTY=0.775718
PRIMER_RIGHT_37_PENALTY=0.766499
PRIMER_LEFT_37_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_37_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_37=87,20
PRIMER_RIGHT_37=244,20
PRIMER_LEFT_37_TM=60.776
PRIMER_RIGHT_37_TM=60.766
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=8.00
PRIMER_LEFT_37_SELF_END=3.00
PRIMER_RIGHT_37_SELF_END=6.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=5.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=158
PRIMER_PAIR_37_PRODUCT_TM=66.7
PRIMER_PAIR_38_PENALTY=1.542217
PRIMER_LEFT_38_PENALTY=0.775718
PRIMER_RIGHT_38_PENALTY=0.766499
PRIMER_LEFT_38_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_38_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_38=86,20
PRIMER_RIGHT_38=244,20
PRIMER_LEFT_38_TM=60.776
PRIMER_RIGHT_38_TM=60.766
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=8.00
PRIMER_LEFT_38_SELF_END=2.00
PRIMER_RIGHT_38_SELF_END=6.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=5.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=159
PRIMER_PAIR_38_PRODUCT_TM=67.0
PRIMER_PAIR_39_PENALTY=1.543141
PRIMER_LEFT_39_PENALTY=1.210088
PRIMER_RIGHT_39_PENALTY=0.333053
PRIMER_LEFT_39_SEQUENCE=TGACAGGCAATTTCAAGGC
PRIMER_RIGHT_39_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_39=87,19
PRIMER_RIGHT_39=238,20
PRIMER_LEFT_39_TM=59.790
PRIMER_RIGHT_39_TM=60.333
PRIMER_LEFT_39_GC_PERCENT=47.368
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=2.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=152
PRIMER_PAIR_39_PRODUCT_TM=66.5
=
SEQUENCE_ID=08.RHAP34FE8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
SEQUENCE_INCLUDED_REGION=20,281
SEQUENCE_TARGET=119,62
PRIMER_LEFT_NUM_RETURNED=40
PRIMER_RIGHT_NUM_RETURNED=40
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=40
PRIMER_PAIR_0_PENALTY=0.218990
PRIMER_LEFT_0_PENALTY=0.201280
PRIMER_RIGHT_0_PENALTY=0.017711
PRIMER_LEFT_0_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_0_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_0=53,20
PRIMER_RIGHT_0=220,20
PRIMER_LEFT_0_TM=59.799
PRIMER_RIGHT_0_TM=59.982
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=168
PRIMER_PAIR_0_PRODUCT_TM=68.8
PRIMER_PAIR_1_PENALTY=0.267700
PRIMER_LEFT_1_PENALTY=0.249989
PRIMER_RIGHT_1_PENALTY=0.017711
PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_1=83,20
PRIMER_RIGHT_1=220,20
PRIMER_LEFT_1_TM=59.750
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=66.5
PRIMER_PAIR_2_PENALTY=0.357340
PRIMER_LEFT_2_PENALTY=0.201280
PRIMER_RIGHT_2_PENALTY=0.156061
PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_2=53,20
PRIMER_RIGHT_2=218,20
PRIMER_LEFT_2_TM=59.799
PRIMER_RIGHT_2_TM=59.844
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=68.6
PRIMER_PAIR_3_PENALTY=0.406050
PRIMER_LEFT_3_PENALTY=0.249989
PRIMER_RIGHT_3_PENALTY=0.156061
PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_3=83,20
PRIMER_RIGHT_3=218,20
PRIMER_LEFT_3_TM=59.750
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=136
PRIMER_PAIR_3_PRODUCT_TM=66.3
PRIMER_PAIR_4_PENALTY=0.440598
PRIMER_LEFT_4_PENALTY=0.422888
PRIMER_RIGHT_4_PENALTY=0.017711
PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_4=49,20
PRIMER_RIGHT_4=220,20
PRIMER_LEFT_4_TM=60.423
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=69.0
PRIMER_PAIR_5_PENALTY=0.510151
PRIMER_LEFT_5_PENALTY=0.201280
PRIMER_RIGHT_5_PENALTY=0.308871
PRIMER_LEFT_5_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_5_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_5=53,20
PRIMER_RIGHT_5=217,20
PRIMER_LEFT_5_TM=59.799
PRIMER_RIGHT_5_TM=59.691
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=5.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=165
PRIMER_PAIR_5_PRODUCT_TM=68.4
PRIMER_PAIR_6_PENALTY=0.543290
PRIMER_LEFT_6_PENALTY=0.525579
PRIMER_RIGHT_6_PENALTY=0.017711
PRIMER_LEFT_6_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_6_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_6=82,20
PRIMER_RIGHT_6=220,20
PRIMER_LEFT_6_TM=60.526
PRIMER_RIGHT_6_TM=59.982
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=4.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=139
PRIMER_PAIR_6_PRODUCT_TM=66.8
PRIMER_PAIR_7_PENALTY=0.558861
PRIMER_LEFT_7_PENALTY=0.249989
PRIMER_RIGHT_7_PENALTY=0.308871
PRIMER_LEFT_7_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_7_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_7=83,20
PRIMER_RIGHT_7=217,20
PRIMER_LEFT_7_TM=59.750
PRIMER_RIGHT_7_TM=59.691
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=7.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=135
PRIMER_PAIR_7_PRODUCT_TM=66.1
PRIMER_PAIR_8_PENALTY=0.578948
PRIMER_LEFT_8_PENALTY=0.422888
PRIMER_RIGHT_8_PENALTY=0.156061
PRIMER_LEFT_8_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_8_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_8=49,20
PRIMER_RIGHT_8=218,20
PRIMER_LEFT_8_TM=60.423
PRIMER_RIGHT_8_TM=59.844
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=170
PRIMER_PAIR_8_PRODUCT_TM=68.9
PRIMER_PAIR_9_PENALTY=0.681640
PRIMER_LEFT_9_PENALTY=0.525579
PRIMER_RIGHT_9_PENALTY=0.156061
PRIMER_LEFT_9_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_9_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_9=82,20
PRIMER_RIGHT_9=218,20
PRIMER_LEFT_9_TM=60.526
PRIMER_RIGHT_9_TM=59.844
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=137
PRIMER_PAIR_9_PRODUCT_TM=66.6
PRIMER_PAIR_10_PENALTY=0.731759
PRIMER_LEFT_10_PENALTY=0.422888
PRIMER_RIGHT_10_PENALTY=0.308871
PRIMER_LEFT_10_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_10_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_10=49,20
PRIMER_RIGHT_10=217,20
PRIMER_LEFT_10_TM=60.423
PRIMER_RIGHT_10_TM=59.691
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=169
PRIMER_PAIR_10_PRODUCT_TM=68.7
PRIMER_PAIR_11_PENALTY=0.834451
PRIMER_LEFT_11_PENALTY=0.525579
PRIMER_RIGHT_11_PENALTY=0.308871
PRIMER_LEFT_11_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_11_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_11=82,20
PRIMER_RIGHT_11=217,20
PRIMER_LEFT_11_TM=60.526
PRIMER_RIGHT_11_TM=59.691
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=136
PRIMER_PAIR_11_PRODUCT_TM=66.3
PRIMER_PAIR_12_PENALTY=0.970916
PRIMER_LEFT_12_PENALTY=0.201280
PRIMER_RIGHT_12_PENALTY=0.769636
PRIMER_LEFT_12_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_12_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_12=53,20
PRIMER_RIGHT_12=210,20
PRIMER_LEFT_12_TM=59.799
PRIMER_RIGHT_12_TM=60.770
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=5.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=158
PRIMER_PAIR_12_PRODUCT_TM=68.0
PRIMER_PAIR_13_PENALTY=1.019625
PRIMER_LEFT_13_PENALTY=0.249989
PRIMER_RIGHT_13_PENALTY=0.769636
PRIMER_LEFT_13_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_13_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_13=83,20
PRIMER_RIGHT_13=210,20
PRIMER_LEFT_13_TM=59.750
PRIMER_RIGHT_13_TM=60.770
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=4.00
PRIMER_PAIR_13_PRODUCT_SIZE=128
PRIMER_PAIR_13_PRODUCT_TM=65.5
PRIMER_PAIR_14_PENALTY=1.044583
PRIMER_LEFT_14_PENALTY=1.026872
PRIMER_RIGHT_14_PENALTY=0.017711
PRIMER_LEFT_14_SEQUENCE=CCACGGATTATCCTTCCATTT
PRIMER_RIGHT_14_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_14=85,21
PRIMER_RIGHT_14=220,20
PRIMER_LEFT_14_TM=60.027
PRIMER_RIGHT_14_TM=59.982
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=4.00
PRIMER_LEFT_14_END_STABILITY=7.2000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=136
PRIMER_PAIR_14_PRODUCT_TM=66.3
PRIMER_PAIR_15_PENALTY=1.049246
PRIMER_LEFT_15_PENALTY=1.031536
PRIMER_RIGHT_15_PENALTY=0.017711
PRIMER_LEFT_15_SEQUENCE=GACCACGGATTATCCTTCCAT
PRIMER_RIGHT_15_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_15=83,21
PRIMER_RIGHT_15=220,20
PRIMER_LEFT_15_TM=60.032
PRIMER_RIGHT_15_TM=59.982
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=4.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=138
PRIMER_PAIR_15_PRODUCT_TM=66.5
PRIMER_PAIR_16_PENALTY=1.116938
PRIMER_LEFT_16_PENALTY=1.099227
PRIMER_RIGHT_16_PENALTY=0.017711
PRIMER_LEFT_16_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_16_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_16=46,20
PRIMER_RIGHT_16=220,20
PRIMER_LEFT_16_TM=58.901
PRIMER_RIGHT_16_TM=59.982
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=4.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=175
PRIMER_PAIR_16_PRODUCT_TM=69.1
PRIMER_PAIR_17_PENALTY=1.153761
PRIMER_LEFT_17_PENALTY=1.136051
PRIMER_RIGHT_17_PENALTY=0.017711
PRIMER_LEFT_17_SEQUENCE=GGTATAGCCTTAGGACCACGG
PRIMER_RIGHT_17_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_17=70,21
PRIMER_RIGHT_17=220,20
PRIMER_LEFT_17_TM=59.864
PRIMER_RIGHT_17_TM=59.982
PRIMER_LEFT_17_GC_PERCENT=57.143
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=151
PRIMER_PAIR_17_PRODUCT_TM=67.6
PRIMER_PAIR_18_PENALTY=1.182933
PRIMER_LEFT_18_PENALTY=1.026872
PRIMER_RIGHT_18_PENALTY=0.156061
PRIMER_LEFT_18_SEQUENCE=CCACGGATTATCCTTCCATTT
PRIMER_RIGHT_18_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_18=85,21
PRIMER_RIGHT_18=218,20
PRIMER_LEFT_18_TM=60.027
PRIMER_RIGHT_18_TM=59.844
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_END_STABILITY=7.2000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=134
PRIMER_PAIR_18_PRODUCT_TM=66.1
PRIMER_PAIR_19_PENALTY=1.187596
PRIMER_LEFT_19_PENALTY=1.031536
PRIMER_RIGHT_19_PENALTY=0.156061
PRIMER_LEFT_19_SEQUENCE=GACCACGGATTATCCTTCCAT
PRIMER_RIGHT_19_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_19=83,21
PRIMER_RIGHT_19=218,20
PRIMER_LEFT_19_TM=60.032
PRIMER_RIGHT_19_TM=59.844
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=136
PRIMER_PAIR_19_PRODUCT_TM=66.3
PRIMER_PAIR_20_PENALTY=1.192524
PRIMER_LEFT_20_PENALTY=0.422888
PRIMER_RIGHT_20_PENALTY=0.769636
PRIMER_LEFT_20_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_20_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_20=49,20
PRIMER_RIGHT_20=210,20
PRIMER_LEFT_20_TM=60.423
PRIMER_RIGHT_20_TM=60.770
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=2.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=162
PRIMER_PAIR_20_PRODUCT_TM=68.3
PRIMER_PAIR_21_PENALTY=1.201715
PRIMER_LEFT_21_PENALTY=1.184005
PRIMER_RIGHT_21_PENALTY=0.017711
PRIMER_LEFT_21_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
PRIMER_RIGHT_21_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_21=53,21
PRIMER_RIGHT_21=220,20
PRIMER_LEFT_21_TM=59.816
PRIMER_RIGHT_21_TM=59.982
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=7.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=3.00
PRIMER_RIGHT_21_SELF_END=4.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=168
PRIMER_PAIR_21_PRODUCT_TM=68.8
PRIMER_PAIR_22_PENALTY=1.205374
PRIMER_LEFT_22_PENALTY=1.187663
PRIMER_RIGHT_22_PENALTY=0.017711
PRIMER_LEFT_22_SEQUENCE=GCCTTAGGACCACGGATTATC
PRIMER_RIGHT_22_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_22=76,21
PRIMER_RIGHT_22=220,20
PRIMER_LEFT_22_TM=59.812
PRIMER_RIGHT_22_TM=59.982
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=5.00
PRIMER_RIGHT_22_SELF_END=4.00
PRIMER_LEFT_22_END_STABILITY=5.9000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=145
PRIMER_PAIR_22_PRODUCT_TM=67.4
PRIMER_PAIR_23_PENALTY=1.255288
PRIMER_LEFT_23_PENALTY=1.099227
PRIMER_RIGHT_23_PENALTY=0.156061
PRIMER_LEFT_23_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_23_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_23=46,20
PRIMER_RIGHT_23=218,20
PRIMER_LEFT_23_TM=58.901
PRIMER_RIGHT_23_TM=59.844
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=3.00
PRIMER_LEFT_23_END_STABILITY=7.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=173
PRIMER_PAIR_23_PRODUCT_TM=69.0
PRIMER_PAIR_24_PENALTY=1.255443
PRIMER_LEFT_24_PENALTY=0.201280
PRIMER_RIGHT_24_PENALTY=1.054163
PRIMER_LEFT_24_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_24_SEQUENCE=AAAGGACCTGGTGGGACAG
PRIMER_LEFT_24=53,20
PRIMER_RIGHT_24=211,19
PRIMER_LEFT_24_TM=59.799
PRIMER_RIGHT_24_TM=59.946
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=57.895
PRIMER_LEFT_24_SELF_ANY=7.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=5.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=159
PRIMER_PAIR_24_PRODUCT_TM=68.0
PRIMER_PAIR_25_PENALTY=1.292112
PRIMER_LEFT_25_PENALTY=1.136051
PRIMER_RIGHT_25_PENALTY=0.156061
PRIMER_LEFT_25_SEQUENCE=GGTATAGCCTTAGGACCACGG
PRIMER_RIGHT_25_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_25=70,21
PRIMER_RIGHT_25=218,20
PRIMER_LEFT_25_TM=59.864
PRIMER_RIGHT_25_TM=59.844
PRIMER_LEFT_25_GC_PERCENT=57.143
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=3.00
PRIMER_RIGHT_25_SELF_END=3.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=149
PRIMER_PAIR_25_PRODUCT_TM=67.4
PRIMER_PAIR_26_PENALTY=1.295215
PRIMER_LEFT_26_PENALTY=0.525579
PRIMER_RIGHT_26_PENALTY=0.769636
PRIMER_LEFT_26_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_26_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_26=82,20
PRIMER_RIGHT_26=210,20
PRIMER_LEFT_26_TM=60.526
PRIMER_RIGHT_26_TM=60.770
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=7.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=3.00
PRIMER_RIGHT_26_SELF_END=2.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=4.00
PRIMER_PAIR_26_PRODUCT_SIZE=129
PRIMER_PAIR_26_PRODUCT_TM=65.7
PRIMER_PAIR_27_PENALTY=1.302983
PRIMER_LEFT_27_PENALTY=1.285273
PRIMER_RIGHT_27_PENALTY=0.017711
PRIMER_LEFT_27_SEQUENCE=CCACGGATTATCCTTCCATT
PRIMER_RIGHT_27_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_27=85,20
PRIMER_RIGHT_27=220,20
PRIMER_LEFT_27_TM=58.715
PRIMER_RIGHT_27_TM=59.982
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=7.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=4.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=136
PRIMER_PAIR_27_PRODUCT_TM=66.3
PRIMER_PAIR_28_PENALTY=1.304152
PRIMER_LEFT_28_PENALTY=0.249989
PRIMER_RIGHT_28_PENALTY=1.054163
PRIMER_LEFT_28_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_28_SEQUENCE=AAAGGACCTGGTGGGACAG
PRIMER_LEFT_28=83,20
PRIMER_RIGHT_28=211,19
PRIMER_LEFT_28_TM=59.750
PRIMER_RIGHT_28_TM=59.946
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=57.895
PRIMER_LEFT_28_SELF_ANY=7.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=129
PRIMER_PAIR_28_PRODUCT_TM=65.4
PRIMER_PAIR_29_PENALTY=1.305530
PRIMER_LEFT_29_PENALTY=1.287819
PRIMER_RIGHT_29_PENALTY=0.017711
PRIMER_LEFT_29_SEQUENCE=AGCTTTTTCCCCTTAGGACC
PRIMER_RIGHT_29_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_29=48,20
PRIMER_RIGHT_29=220,20
PRIMER_LEFT_29_TM=58.712
PRIMER_RIGHT_29_TM=59.982
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=3.00
PRIMER_RIGHT_29_SELF_END=4.00
PRIMER_LEFT_29_END_STABILITY=9.1000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=173
PRIMER_PAIR_29_PRODUCT_TM=69.0
PRIMER_PAIR_30_PENALTY=1.305884
PRIMER_LEFT_30_PENALTY=1.288173
PRIMER_RIGHT_30_PENALTY=0.017711
PRIMER_LEFT_30_SEQUENCE=TCAGCTTTTTCCCCTTAGGAC
PRIMER_RIGHT_30_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_30=46,21
PRIMER_RIGHT_30=220,20
PRIMER_LEFT_30_TM=59.712
PRIMER_RIGHT_30_TM=59.982
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=4.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=175
PRIMER_PAIR_30_PRODUCT_TM=69.1
PRIMER_PAIR_31_PENALTY=1.335744
PRIMER_LEFT_31_PENALTY=1.026872
PRIMER_RIGHT_31_PENALTY=0.308871
PRIMER_LEFT_31_SEQUENCE=CCACGGATTATCCTTCCATTT
PRIMER_RIGHT_31_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_31=85,21
PRIMER_RIGHT_31=217,20
PRIMER_LEFT_31_TM=60.027
PRIMER_RIGHT_31_TM=59.691
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=7.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.2000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=133
PRIMER_PAIR_31_PRODUCT_TM=65.9
PRIMER_PAIR_32_PENALTY=1.339296
PRIMER_LEFT_32_PENALTY=1.183236
PRIMER_RIGHT_32_PENALTY=0.156061
PRIMER_LEFT_32_SEQUENCE=TTCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_32_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_32=45,21
PRIMER_RIGHT_32=218,20
PRIMER_LEFT_32_TM=60.183
PRIMER_RIGHT_32_TM=59.844
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=3.00
PRIMER_RIGHT_32_SELF_END=3.00
PRIMER_LEFT_32_END_STABILITY=7.2000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=174
PRIMER_PAIR_32_PRODUCT_TM=68.9
PRIMER_PAIR_33_PENALTY=1.340065
PRIMER_LEFT_33_PENALTY=1.184005
PRIMER_RIGHT_33_PENALTY=0.156061
PRIMER_LEFT_33_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
PRIMER_RIGHT_33_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_33=53,21
PRIMER_RIGHT_33=218,20
PRIMER_LEFT_33_TM=59.816
PRIMER_RIGHT_33_TM=59.844
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=7.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=3.00
PRIMER_RIGHT_33_SELF_END=3.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=166
PRIMER_PAIR_33_PRODUCT_TM=68.6
PRIMER_PAIR_34_PENALTY=1.340407
PRIMER_LEFT_34_PENALTY=1.031536
PRIMER_RIGHT_34_PENALTY=0.308871
PRIMER_LEFT_34_SEQUENCE=GACCACGGATTATCCTTCCAT
PRIMER_RIGHT_34_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_34=83,21
PRIMER_RIGHT_34=217,20
PRIMER_LEFT_34_TM=60.032
PRIMER_RIGHT_34_TM=59.691
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=7.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=2.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=135
PRIMER_PAIR_34_PRODUCT_TM=66.1
PRIMER_PAIR_35_PENALTY=1.343724
PRIMER_LEFT_35_PENALTY=1.187663
PRIMER_RIGHT_35_PENALTY=0.156061
PRIMER_LEFT_35_SEQUENCE=GCCTTAGGACCACGGATTATC
PRIMER_RIGHT_35_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_35=76,21
PRIMER_RIGHT_35=218,20
PRIMER_LEFT_35_TM=59.812
PRIMER_RIGHT_35_TM=59.844
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=5.00
PRIMER_RIGHT_35_SELF_END=3.00
PRIMER_LEFT_35_END_STABILITY=5.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=143
PRIMER_PAIR_35_PRODUCT_TM=67.2
PRIMER_PAIR_36_PENALTY=1.347129
PRIMER_LEFT_36_PENALTY=1.329419
PRIMER_RIGHT_36_PENALTY=0.017711
PRIMER_LEFT_36_SEQUENCE=AGCCTTAGGACCACGGATTA
PRIMER_RIGHT_36_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_36=75,20
PRIMER_RIGHT_36=220,20
PRIMER_LEFT_36_TM=58.671
PRIMER_RIGHT_36_TM=59.982
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=2.00
PRIMER_RIGHT_36_SELF_END=4.00
PRIMER_LEFT_36_END_STABILITY=5.9000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=146
PRIMER_PAIR_36_PRODUCT_TM=67.3
PRIMER_PAIR_37_PENALTY=1.347129
PRIMER_LEFT_37_PENALTY=1.329419
PRIMER_RIGHT_37_PENALTY=0.017711
PRIMER_LEFT_37_SEQUENCE=TAGCCTTAGGACCACGGATT
PRIMER_RIGHT_37_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_37=74,20
PRIMER_RIGHT_37=220,20
PRIMER_LEFT_37_TM=58.671
PRIMER_RIGHT_37_TM=59.982
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=4.00
PRIMER_LEFT_37_END_STABILITY=8.1000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=147
PRIMER_PAIR_37_PRODUCT_TM=67.3
PRIMER_PAIR_38_PENALTY=1.408099
PRIMER_LEFT_38_PENALTY=1.099227
PRIMER_RIGHT_38_PENALTY=0.308871
PRIMER_LEFT_38_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_38_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_38=46,20
PRIMER_RIGHT_38=217,20
PRIMER_LEFT_38_TM=58.901
PRIMER_RIGHT_38_TM=59.691
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=3.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.2000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=172
PRIMER_PAIR_38_PRODUCT_TM=68.8
PRIMER_PAIR_39_PENALTY=1.441333
PRIMER_LEFT_39_PENALTY=1.285273
PRIMER_RIGHT_39_PENALTY=0.156061
PRIMER_LEFT_39_SEQUENCE=CCACGGATTATCCTTCCATT
PRIMER_RIGHT_39_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_39=85,20
PRIMER_RIGHT_39=218,20
PRIMER_LEFT_39_TM=58.715
PRIMER_RIGHT_39_TM=59.844
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=7.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=3.00
PRIMER_LEFT_39_END_STABILITY=8.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=134
PRIMER_PAIR_39_PRODUCT_TM=66.1
=
PRIMER_NUM_RETURN=8
SEQUENCE_ID=09.RHAP33FA9.seq
SEQUENCE_TEMPLATE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
SEQUENCE_INCLUDED_REGION=0,371
SEQUENCE_TARGET=151,18
SEQUENCE_TARGET=192,53
SEQUENCE_TARGET=248,16
PRIMER_LEFT_NUM_RETURNED=8
PRIMER_RIGHT_NUM_RETURNED=8
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=8
PRIMER_PAIR_0_PENALTY=8.299756
PRIMER_LEFT_0_PENALTY=4.294975
PRIMER_RIGHT_0_PENALTY=4.004782
PRIMER_LEFT_0_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
PRIMER_RIGHT_0_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_0=19,24
PRIMER_RIGHT_0=191,18
PRIMER_LEFT_0_TM=60.295
PRIMER_RIGHT_0_TM=62.005
PRIMER_LEFT_0_GC_PERCENT=45.833
PRIMER_RIGHT_0_GC_PERCENT=72.222
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=173
PRIMER_PAIR_0_PRODUCT_TM=72.1
PRIMER_PAIR_1_PENALTY=8.306042
PRIMER_LEFT_1_PENALTY=4.301260
PRIMER_RIGHT_1_PENALTY=4.004782
PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_1=67,24
PRIMER_RIGHT_1=191,18
PRIMER_LEFT_1_TM=60.301
PRIMER_RIGHT_1_TM=62.005
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=72.222
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_1_PRODUCT_TM=70.5
PRIMER_PAIR_2_PENALTY=8.589410
PRIMER_LEFT_2_PENALTY=4.584629
PRIMER_RIGHT_2_PENALTY=4.004782
PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_2=20,24
PRIMER_RIGHT_2=191,18
PRIMER_LEFT_2_TM=59.415
PRIMER_RIGHT_2_TM=62.005
PRIMER_LEFT_2_GC_PERCENT=45.833
PRIMER_RIGHT_2_GC_PERCENT=72.222
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=172
PRIMER_PAIR_2_PRODUCT_TM=72.1
PRIMER_PAIR_3_PENALTY=8.956691
PRIMER_LEFT_3_PENALTY=4.951910
PRIMER_RIGHT_3_PENALTY=4.004782
PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=191,18
PRIMER_LEFT_3_TM=57.048
PRIMER_RIGHT_3_TM=62.005
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=72.222
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=72.0
PRIMER_PAIR_4_PENALTY=9.135884
PRIMER_LEFT_4_PENALTY=5.131103
PRIMER_RIGHT_4_PENALTY=4.004782
PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_4=48,23
PRIMER_RIGHT_4=191,18
PRIMER_LEFT_4_TM=57.869
PRIMER_RIGHT_4_TM=62.005
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=72.222
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=144
PRIMER_PAIR_4_PRODUCT_TM=71.1
PRIMER_PAIR_5_PENALTY=9.202456
PRIMER_LEFT_5_PENALTY=5.197674
PRIMER_RIGHT_5_PENALTY=4.004782
PRIMER_LEFT_5_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCC
PRIMER_RIGHT_5_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_5=67,23
PRIMER_RIGHT_5=191,18
PRIMER_LEFT_5_TM=57.802
PRIMER_RIGHT_5_TM=62.005
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=72.222
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=1.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=125
PRIMER_PAIR_5_PRODUCT_TM=70.5
PRIMER_PAIR_6_PENALTY=9.279205
PRIMER_LEFT_6_PENALTY=5.274423
PRIMER_RIGHT_6_PENALTY=4.004782
PRIMER_LEFT_6_SEQUENCE=GACATCCTGTTACCATGTAGGAGTT
PRIMER_RIGHT_6_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_6=22,25
PRIMER_RIGHT_6=191,18
PRIMER_LEFT_6_TM=59.726
PRIMER_RIGHT_6_TM=62.005
PRIMER_LEFT_6_GC_PERCENT=44.000
PRIMER_RIGHT_6_GC_PERCENT=72.222
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=1.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=170
PRIMER_PAIR_6_PRODUCT_TM=72.0
PRIMER_PAIR_7_PENALTY=9.284951
PRIMER_LEFT_7_PENALTY=5.280169
PRIMER_RIGHT_7_PENALTY=4.004782
PRIMER_LEFT_7_SEQUENCE=CTGCTAAGTAATGAGTTCAAAGCC
PRIMER_RIGHT_7_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_7=66,24
PRIMER_RIGHT_7=191,18
PRIMER_LEFT_7_TM=58.720
PRIMER_RIGHT_7_TM=62.005
PRIMER_LEFT_7_GC_PERCENT=41.667
PRIMER_RIGHT_7_GC_PERCENT=72.222
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=1.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=126
PRIMER_PAIR_7_PRODUCT_TM=70.8
=
SEQUENCE_ID=09.RHAP34FA9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
SEQUENCE_INCLUDED_REGION=20,280
SEQUENCE_TARGET=44,42
PRIMER_LEFT_NUM_RETURNED=8
PRIMER_RIGHT_NUM_RETURNED=8
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=8
PRIMER_PAIR_0_PENALTY=7.200927
PRIMER_LEFT_0_PENALTY=6.971746
PRIMER_RIGHT_0_PENALTY=0.229181
PRIMER_LEFT_0_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_0_SEQUENCE=TTCCTCACAGAGGGAACAGG
PRIMER_LEFT_0=20,24
PRIMER_RIGHT_0=166,20
PRIMER_LEFT_0_TM=57.028
PRIMER_RIGHT_0_TM=60.229
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=147
PRIMER_PAIR_0_PRODUCT_TM=69.2
PRIMER_PAIR_1_PENALTY=7.687872
PRIMER_LEFT_1_PENALTY=6.971746
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
PRIMER_LEFT_1=20,24
PRIMER_RIGHT_1=150,20
PRIMER_LEFT_1_TM=57.028
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=131
PRIMER_PAIR_1_PRODUCT_TM=67.8
PRIMER_PAIR_2_PENALTY=7.830958
PRIMER_LEFT_2_PENALTY=6.971746
PRIMER_RIGHT_2_PENALTY=0.859212
PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
PRIMER_LEFT_2=20,24
PRIMER_RIGHT_2=151,20
PRIMER_LEFT_2_TM=57.028
PRIMER_RIGHT_2_TM=59.141
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=132
PRIMER_PAIR_2_PRODUCT_TM=67.8
PRIMER_PAIR_3_PENALTY=7.830958
PRIMER_LEFT_3_PENALTY=6.971746
PRIMER_RIGHT_3_PENALTY=0.859212
PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
PRIMER_LEFT_3=20,24
PRIMER_RIGHT_3=153,20
PRIMER_LEFT_3_TM=57.028
PRIMER_RIGHT_3_TM=59.141
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=134
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=8.039099
PRIMER_LEFT_4_PENALTY=6.971746
PRIMER_RIGHT_4_PENALTY=1.067353
PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
PRIMER_LEFT_4=20,24
PRIMER_RIGHT_4=147,21
PRIMER_LEFT_4_TM=57.028
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=128
PRIMER_PAIR_4_PRODUCT_TM=67.4
PRIMER_PAIR_5_PENALTY=8.039099
PRIMER_LEFT_5_PENALTY=6.971746
PRIMER_RIGHT_5_PENALTY=1.067353
PRIMER_LEFT_5_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_5_SEQUENCE=GGGGCTGTTTCTGATTCTTTC
PRIMER_LEFT_5=20,24
PRIMER_RIGHT_5=148,21
PRIMER_LEFT_5_TM=57.028
PRIMER_RIGHT_5_TM=60.067
PRIMER_LEFT_5_GC_PERCENT=33.333
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=4.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.8000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=129
PRIMER_PAIR_5_PRODUCT_TM=67.6
PRIMER_PAIR_6_PENALTY=8.096344
PRIMER_LEFT_6_PENALTY=6.971746
PRIMER_RIGHT_6_PENALTY=1.124598
PRIMER_LEFT_6_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_6_SEQUENCE=ACAGGGGCTGTTTCTGATTCT
PRIMER_LEFT_6=20,24
PRIMER_RIGHT_6=151,21
PRIMER_LEFT_6_TM=57.028
PRIMER_RIGHT_6_TM=60.125
PRIMER_LEFT_6_GC_PERCENT=33.333
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=4.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.8000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=132
PRIMER_PAIR_6_PRODUCT_TM=67.8
PRIMER_PAIR_7_PENALTY=8.199338
PRIMER_LEFT_7_PENALTY=6.971746
PRIMER_RIGHT_7_PENALTY=1.227592
PRIMER_LEFT_7_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_7_SEQUENCE=TTTGTGTTGCTTGCATTTTTG
PRIMER_LEFT_7=20,24
PRIMER_RIGHT_7=192,21
PRIMER_LEFT_7_TM=57.028
PRIMER_RIGHT_7_TM=59.772
PRIMER_LEFT_7_GC_PERCENT=33.333
PRIMER_RIGHT_7_GC_PERCENT=33.333
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=5.8000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=173
PRIMER_PAIR_7_PRODUCT_TM=69.9
=
SEQUENCE_ID=10.RHAP34FA10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=12,236
SEQUENCE_TARGET=155,32
PRIMER_LEFT_NUM_RETURNED=8
PRIMER_RIGHT_NUM_RETURNED=8
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=8
PRIMER_PAIR_0_PENALTY=1.750634
PRIMER_LEFT_0_PENALTY=0.649665
PRIMER_RIGHT_0_PENALTY=1.100969
PRIMER_LEFT_0_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_0_SEQUENCE=CAAGGACAGAACTGAACTGGC
PRIMER_LEFT_0=101,20
PRIMER_RIGHT_0=229,21
PRIMER_LEFT_0_TM=59.350
PRIMER_RIGHT_0_TM=59.899
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=129
PRIMER_PAIR_0_PRODUCT_TM=66.1
PRIMER_PAIR_1_PENALTY=1.750634
PRIMER_LEFT_1_PENALTY=0.649665
PRIMER_RIGHT_1_PENALTY=1.100969
PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
PRIMER_LEFT_1=101,20
PRIMER_RIGHT_1=230,21
PRIMER_LEFT_1_TM=59.350
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=130
PRIMER_PAIR_1_PRODUCT_TM=66.3
PRIMER_PAIR_2_PENALTY=2.364169
PRIMER_LEFT_2_PENALTY=1.263200
PRIMER_RIGHT_2_PENALTY=1.100969
PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
PRIMER_LEFT_2=96,21
PRIMER_RIGHT_2=229,21
PRIMER_LEFT_2_TM=59.737
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=134
PRIMER_PAIR_2_PRODUCT_TM=66.1
PRIMER_PAIR_3_PENALTY=2.364169
PRIMER_LEFT_3_PENALTY=1.263200
PRIMER_RIGHT_3_PENALTY=1.100969
PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
PRIMER_LEFT_3=96,21
PRIMER_RIGHT_3=230,21
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.899
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=135
PRIMER_PAIR_3_PRODUCT_TM=66.4
PRIMER_PAIR_4_PENALTY=2.578428
PRIMER_LEFT_4_PENALTY=0.649665
PRIMER_RIGHT_4_PENALTY=1.928763
PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
PRIMER_LEFT_4=101,20
PRIMER_RIGHT_4=231,21
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=59.071
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=131
PRIMER_PAIR_4_PRODUCT_TM=66.3
PRIMER_PAIR_5_PENALTY=2.673857
PRIMER_LEFT_5_PENALTY=1.427458
PRIMER_RIGHT_5_PENALTY=1.246400
PRIMER_LEFT_5_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
PRIMER_RIGHT_5_SEQUENCE=GGCTCCTACAACCTGTCCTCT
PRIMER_LEFT_5=37,21
PRIMER_RIGHT_5=211,21
PRIMER_LEFT_5_TM=59.573
PRIMER_RIGHT_5_TM=59.754
PRIMER_LEFT_5_GC_PERCENT=38.095
PRIMER_RIGHT_5_GC_PERCENT=57.143
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=175
PRIMER_PAIR_5_PRODUCT_TM=66.8
PRIMER_PAIR_6_PENALTY=2.683392
PRIMER_LEFT_6_PENALTY=1.427458
PRIMER_RIGHT_6_PENALTY=1.255934
PRIMER_LEFT_6_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
PRIMER_RIGHT_6_SEQUENCE=GGCTCCTACAACCTGTCCTC
PRIMER_LEFT_6=37,21
PRIMER_RIGHT_6=211,20
PRIMER_LEFT_6_TM=59.573
PRIMER_RIGHT_6_TM=58.744
PRIMER_LEFT_6_GC_PERCENT=38.095
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=175
PRIMER_PAIR_6_PRODUCT_TM=66.8
PRIMER_PAIR_7_PENALTY=2.727005
PRIMER_LEFT_7_PENALTY=0.649665
PRIMER_RIGHT_7_PENALTY=2.077340
PRIMER_LEFT_7_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_7_SEQUENCE=AAGGACAGAACTGAACTGGCTC
PRIMER_LEFT_7=101,20
PRIMER_RIGHT_7=228,22
PRIMER_LEFT_7_TM=59.350
PRIMER_RIGHT_7_TM=59.923
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=128
PRIMER_PAIR_7_PRODUCT_TM=65.8
=
SEQUENCE_ID=13.RHAP34FB1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,244
SEQUENCE_TARGET=22,16
SEQUENCE_TARGET=57,34
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=14.RHAP34FB2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
SEQUENCE_INCLUDED_REGION=13,303
SEQUENCE_TARGET=177,33
SEQUENCE_TARGET=254,14
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
