P3_SETTINGS_FILE_USED=../test/primer_high_gc_load_set.set
P3_FILE_TYPE=settings
P3_FILE_ID=Test with higher Tm
PRIMER_MIN_GC=62
PRIMER_OPT_GC_PERCENT=75
PRIMER_MAX_GC=80
PRIMER_WT_GC_PERCENT_GT=1.0
PRIMER_WT_GC_PERCENT_LT=1.0
P3_SETTINGS_FILE_END=
SEQUENCE_ID=Load qPCR settings
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=5
PRIMER_TASK=pick_detection_primers
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=28.724193
PRIMER_LEFT_0_PENALTY=15.801623
PRIMER_RIGHT_0_PENALTY=12.922570
PRIMER_LEFT_0_SEQUENCE=cggactttggtggagaggg
PRIMER_RIGHT_0_SEQUENCE=agctcaccggaactcccc
PRIMER_LEFT_0=1221,19
PRIMER_RIGHT_0=1362,18
PRIMER_LEFT_0_TM=62.960
PRIMER_RIGHT_0_TM=62.589
PRIMER_LEFT_0_GC_PERCENT=63.158
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=142
PRIMER_PAIR_0_PRODUCT_TM=77.9
PRIMER_PAIR_1_PENALTY=28.724193
PRIMER_LEFT_1_PENALTY=15.801623
PRIMER_RIGHT_1_PENALTY=12.922570
PRIMER_LEFT_1_SEQUENCE=ccggactttggtggagagg
PRIMER_RIGHT_1_SEQUENCE=gctcaccggaactcccct
PRIMER_LEFT_1=1220,19
PRIMER_RIGHT_1=1361,18
PRIMER_LEFT_1_TM=62.960
PRIMER_RIGHT_1_TM=62.589
PRIMER_LEFT_1_GC_PERCENT=63.158
PRIMER_RIGHT_1_GC_PERCENT=66.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=78.2
PRIMER_PAIR_2_PENALTY=28.724193
PRIMER_LEFT_2_PENALTY=15.801623
PRIMER_RIGHT_2_PENALTY=12.922570
PRIMER_LEFT_2_SEQUENCE=ccggactttggtggagagg
PRIMER_RIGHT_2_SEQUENCE=agctcaccggaactcccc
PRIMER_LEFT_2=1220,19
PRIMER_RIGHT_2=1362,18
PRIMER_LEFT_2_TM=62.960
PRIMER_RIGHT_2_TM=62.589
PRIMER_LEFT_2_GC_PERCENT=63.158
PRIMER_RIGHT_2_GC_PERCENT=66.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=143
PRIMER_PAIR_2_PRODUCT_TM=78.1
PRIMER_PAIR_3_PENALTY=28.724193
PRIMER_LEFT_3_PENALTY=15.801623
PRIMER_RIGHT_3_PENALTY=12.922570
PRIMER_LEFT_3_SEQUENCE=cccggactttggtggagag
PRIMER_RIGHT_3_SEQUENCE=gctcaccggaactcccct
PRIMER_LEFT_3=1219,19
PRIMER_RIGHT_3=1361,18
PRIMER_LEFT_3_TM=62.960
PRIMER_RIGHT_3_TM=62.589
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=143
PRIMER_PAIR_3_PRODUCT_TM=78.4
PRIMER_PAIR_4_PENALTY=31.141401
PRIMER_LEFT_4_PENALTY=15.801623
PRIMER_RIGHT_4_PENALTY=15.339778
PRIMER_LEFT_4_SEQUENCE=cggactttggtggagaggg
PRIMER_RIGHT_4_SEQUENCE=gctcaccggaactccccta
PRIMER_LEFT_4=1221,19
PRIMER_RIGHT_4=1361,19
PRIMER_LEFT_4_TM=62.960
PRIMER_RIGHT_4_TM=62.498
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=141
PRIMER_PAIR_4_PRODUCT_TM=78.0
=
