PRIMER PICKING RESULTS FOR pcr_primers

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          1   20   58.23   50.00 10.00  0.00 CCTACAGCTGTGGGAAAATG
   RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
   PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER          0   20   57.27   45.00 12.00  2.00 TCCTACAGCTGTGGGAAAAT
   RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
   PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     0     0     0     0     0     0     7     0     0     0     0     0     3
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 3, ok 3
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR pcr_primers_and_io

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          9   22   59.90   40.91  6.00  4.00 TGTTTCACACTTACGGAAATGG
RIGHT PRIMER       247   20   60.23   50.00  3.00  0.00 TCCTGTTGGAGAAAAGCCAC
INTERNAL OLIGO     141   18   62.80   66.67  3.00  1.00 CTTAGCGTGCGTGGGGTC
SEQUENCE SIZE: 300
INCLUDED REGION SIZE: 300

PRODUCT SIZE: 239, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

    0 NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTAT
               >>>>>>>>>>>>>>>>>>>>>>                             

   60 CCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGC
                                                                  

  120 CTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTC
                           ^^^^^^^^^^^^^^^^^^                     

  180 TGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTC
                                                      <<<<<<<<<<<<

  240 CAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
      <<<<<<<<                                                    

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          9   22   59.90   40.91  6.00  4.00 TGTTTCACACTTACGGAAATGG
   RIGHT PRIMER       246   20   59.33   50.00  3.00  1.00 CCTGTTGGAGAAAAGCCACT
   INTERNAL OLIGO     141   18   62.80   66.67  3.00  1.00 CTTAGCGTGCGTGGGGTC
   PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER          9   22   59.90   40.91  6.00  4.00 TGTTTCACACTTACGGAAATGG
   RIGHT PRIMER       248   20   60.74   45.00  3.00  0.00 TTCCTGTTGGAGAAAAGCCA
   INTERNAL OLIGO     141   18   62.80   66.67  3.00  1.00 CTTAGCGTGCGTGGGGTC
   PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER         11   21   58.13   38.10  6.00  6.00 TTTCACACTTACGGAAATGGA
   RIGHT PRIMER       247   20   60.23   50.00  3.00  0.00 TCCTGTTGGAGAAAAGCCAC
   INTERNAL OLIGO     141   18   62.80   66.67  3.00  1.00 CTTAGCGTGCGTGGGGTC
   PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER         10   22   59.00   40.91  6.00  6.00 GTTTCACACTTACGGAAATGGA
   RIGHT PRIMER       247   20   60.23   50.00  3.00  0.00 TCCTGTTGGAGAAAAGCCAC
   INTERNAL OLIGO     141   18   62.80   66.67  3.00  1.00 CTTAGCGTGCGTGGGGTC
   PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     281   210     0     0     0     0     0    30    29     0     0     0     0    12
Right    284   210     0     0     0     0     0     7    44     0     0     0     0    23
Intl     393   280     0     0     0     0     0    30    46     0     0     3     0    34
Pair Stats:
considered 5, ok 5
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR left_only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER         72   20   59.96   55.00  3.00  0.00 TTAGAAGGGGTGTCTGGGTG
SEQUENCE SIZE: 175
INCLUDED REGION SIZE: 175

TARGETS (start, len)*: 100,20
    0 GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA
                                                                  

   60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG
                  >>>>>>>>>>>>>>>>>>>>        ********************

  120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
                                                             

KEYS (in order of precedence):
****** target
>>>>>> left primer


ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER         64   20   59.73   55.00  6.00  0.00 CTGCAGGTTTAGAAGGGGTG
 2 LEFT_PRIMER         60   20   59.73   55.00  8.00  0.00 CACCCTGCAGGTTTAGAAGG
 3 LEFT_PRIMER         56   20   60.31   55.00  8.00  6.00 AGCACACCCTGCAGGTTTAG
 4 LEFT_PRIMER         65   20   59.59   50.00  4.00  0.00 TGCAGGTTTAGAAGGGGTGT

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left    1121    38    23     0     0     0     0    42   866     0     0     0     0   152
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR right_only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
RIGHT_PRIMER       125   20   60.29   45.00  3.00  1.00 GTGCTTTGCGTTTTGGAAGT
SEQUENCE SIZE: 136
INCLUDED REGION SIZE: 136

TARGETS (start, len)*: 90,10
    0 GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGAC
                                                                  

   60 CTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCA
                                    **********      <<<<<<<<<<<<<<

  120 AAGCACGCTCCCGATC
      <<<<<<          

KEYS (in order of precedence):
****** target
<<<<<< right primer


ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER        75   20   59.27   45.00  2.00  1.00 TTGGCGTTTCTATCAGGTCA
 2 RIGHT_PRIMER       124   20   60.78   40.00  3.00  3.00 TGCTTTGCGTTTTGGAAGTT
 3 RIGHT_PRIMER        76   21   60.13   47.62  2.00  1.00 GTTGGCGTTTCTATCAGGTCA
 4 RIGHT_PRIMER        79   21   60.26   47.62  3.00  0.00 TGAGTTGGCGTTTCTATCAGG

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Right    623    27    36     0     0     5     0   230   145     0     6     0     0   174
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR hyb_probe_only

No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
INTERNAL_OLIGO      64   20   59.73   55.00  6.00  0.00 CTGCAGGTTTAGAAGGGGTG
SEQUENCE SIZE: 175
INCLUDED REGION SIZE: 175

INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 90,20
    0 GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA
                                                                  

   60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG
          ^^^^^^^^^^^^^^^^^^^^      xxxxxxxxxxxxxxxxxxxx          

  120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
                                                             

KEYS (in order of precedence):
xxxxxx excluded region for internal oligo
^^^^^^ internal oligo


ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq
 1 INTERNAL_OLIGO      60   20   59.73   55.00  8.00  0.00 CACCCTGCAGGTTTAGAAGG
 2 INTERNAL_OLIGO      56   20   60.31   55.00  8.00  6.00 AGCACACCCTGCAGGTTTAG
 3 INTERNAL_OLIGO       1   20   60.38   55.00  4.00  0.00 CATCTGCACCCTGTAAACCC
 4 INTERNAL_OLIGO      65   20   59.59   50.00  4.00  0.00 TGCAGGTTTAGAAGGGGTGT

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Intl    1040    27     0    33     0     0     0    44   804     0     0     0     0   132
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR left_only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   20   59.96   55.00  3.00  0.00 TTAGAAGGGGTGTCTGGGTG
SEQUENCE SIZE: 20
INCLUDED REGION SIZE: 20

    0 TTAGAAGGGGTGTCTGGGTG
      >>>>>>>>>>>>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR right_only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   20   59.96   55.00  3.00  0.00 TTAGAAGGGGTGTCTGGGTG
SEQUENCE SIZE: 20
INCLUDED REGION SIZE: 20

    0 TTAGAAGGGGTGTCTGGGTG
      >>>>>>>>>>>>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR hyb_probe_only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   20   59.96   55.00  3.00  0.00 TTAGAAGGGGTGTCTGGGTG
SEQUENCE SIZE: 20
INCLUDED REGION SIZE: 20

    0 TTAGAAGGGGTGTCTGGGTG
      >>>>>>>>>>>>>>>>>>>>

KEYS (in order of precedence):
>>>>>> left primer



Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
primer3 release 2.0.0


