ACCEPTABLE LEFT PRIMERS
                                  0-based     #               self  self  qual-
   # sequence                       start ln  N   GC%     Tm   any   end   lity
   0 TCCTACAGCTGTGGGAAAATG              0 21  0 47.62 60.118 12.00  0.00  1.118
   1 CCTACAGCTGTGGGAAAATG               1 20  0 50.00 58.228 10.00  0.00  1.772
   2 TCCTACAGCTGTGGGAAAAT               0 20  0 45.00 57.268 12.00  2.00  2.732
