PRIMER PICKING RESULTS FOR boundary_test_00

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     0     1
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_01

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_02

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_03

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       4     3     0     0     0     0     0     0     0     0     0     0     0     1
Right      4     3     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, unacceptable product size 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_04

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     0     1
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, unacceptable product size 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_05

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
RIGHT PRIMER        56   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>       <<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     3     0     0     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 4, unacceptable product size 3, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_06

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     5     0     0     0     0     0     0     0     0     0     0     0     0
Right      5     5     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_07

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_08

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ...                                                       .

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       6     6     0     0     0     0     0     0     0     0     0     0     0     0
Right      6     5     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_09

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 54


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                      ...

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     5     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_10

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     5     0     0     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_11

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   61.18   33.33 12.00  9.50 CACGTCATCTNATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.75

    0 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     2     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 3, unacceptable product size 2, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_11

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     5     0     0     0     0     0     0     0     0     0     0     0     0
Right      5     2     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_12

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   63.22   34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_13

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   55.84    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_14

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     1     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_15

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   90.45  100.00  0.00  0.00 GGGGGGGGGGGGGGGGGGGGGGGGG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00

    0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_16

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     1     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     1     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_17

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_18

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ...                                                       .

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     1     0     0     0     0     0     0     0     0     0     0     0     0
Right      1     1     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_19

INPUT PROBLEM: SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE

PRIMER PICKING RESULTS FOR boundary_test_20

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 54


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                      ...

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     1     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_21

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     0     0     0     0     0     0     0     0     0     0     0     1
Right      3     2     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_22

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 26,6

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                ******                           

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       2     2     0     0     0     0     0     0     0     0     0     0     0     0
Right      3     2     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_23

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 27,6

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                 ******                          

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     0     0     0     0     0     0     0     0     0     0     0     1
Right      2     2     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_24

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 28,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  *****                          

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       4     3     0     0     0     0     0     0     0     0     0     0     0     1
Right      2     2     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_25

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,1 28,4

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       4     3     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_26

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 26,1 27,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                ******                           

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     1     0     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_27

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 27,1 28,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                 ******                          

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       4     3     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     1     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_28

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 28,1 29,4

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  *****                          

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     1     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_29

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> *   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       4     3     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_30

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 28,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       4     3     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_31

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 0,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      *                                                          

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, no target 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_32

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 27,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_33

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 26,6

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                XXXXXX                           

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     1     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_34

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 27,6

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                 XXXXXX                          

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     1     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_35

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  XXXXX                          

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     1     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_36

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 27,1 28,4

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_37

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 26,1 27,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                XXXXXX                           

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     1     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_37

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 27,1 28,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                 XXXXXX                          

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     1     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_38

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,1 29,4

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  XXXXX                          

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     1     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_39

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_40

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 28,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_41

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 10,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN

XXXXXX excluded region

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     1     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_42

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_43

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  X                              

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     1     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_44

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_45

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_46

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  X                              

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     1     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_47

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_48

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                  X                              

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     1     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_49

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   63.02   40.00  4.00  0.00 GAATTGTTTGGAAATGACAGTGAGG
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_50

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
                                  X                              

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     1     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_51

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   63.02   40.00  4.00  0.00 GAATTGTTTGGAAATGACAGTGAGG
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_52

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   63.02   40.00  4.00  0.00 GAATTGTTTGGAAATGACAGTGAGG
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_53

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN
                                  X                              

KEYS (in order of precedence):
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, tm diff too large 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_54

No mispriming library specified
Using 0-based sequence positions
WARNING: Unrecognized base in input sequence

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   63.02   40.00  4.00  0.00 GAATTGTTTGGAAATGACAGTGAGG
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
EXCLUDED REGIONS (start, len)*: 28,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>> X   <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_55

INPUT PROBLEM: Unrecognized base in input sequence

PRIMER PICKING RESULTS FOR poly_x_1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 187
INCLUDED REGION SIZE: 187


    0 AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGG
   60 TTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTT
  120 GGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTT
  180 TCCCCCC


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     138     0     0     0     0     0     0     0     0     0     0   138     0     0
Right    138     0     0     0     0     0     0     0     0     0     0   138     0     0
Intl       0     0     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR poly_x_2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         29   25   67.77   40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC
RIGHT PRIMER       130   25   67.77   40.00 12.00  3.00 GAAAAATTTTTCCCCCAAAAAGGGG
INTERNAL OLIGO      87   19   60.50   47.37  5.00  3.00 CCCTTTTTGGGGGAAAAAC
SEQUENCE SIZE: 180
INCLUDED REGION SIZE: 180

PRODUCT SIZE: 102, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 14.00

    0 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGG
                                   >>>>>>>>>>>>>>>>>>>>>>>>>      

   60 AAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
                                 ^^^^^^^^^^^^^^^^^^^<<<<<<<<<<<<<<

  120 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG
      <<<<<<<<<<<                                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         29   25   67.77   40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC
   RIGHT PRIMER       131   25   67.77   40.00 14.00  1.00 GGAAAAATTTTTCCCCCAAAAAGGG
   INTERNAL OLIGO      88   19   60.50   47.37  5.00  1.00 CCTTTTTGGGGGAAAAACC
   PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00

 2 LEFT PRIMER         29   25   67.77   40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC
   RIGHT PRIMER       132   25   67.77   40.00 16.00  1.00 GGGAAAAATTTTTCCCCCAAAAAGG
   INTERNAL OLIGO      89   19   60.50   47.37  5.00  1.00 CTTTTTGGGGGAAAAACCC
   PRODUCT SIZE: 104, PAIR ANY COMPL: 24.00, PAIR 3' COMPL: 22.00

 3 LEFT PRIMER         29   25   67.77   40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC
   RIGHT PRIMER       133   25   67.77   40.00 18.00  3.00 GGGGAAAAATTTTTCCCCCAAAAAG
   INTERNAL OLIGO      89   19   60.50   47.37  5.00  1.00 CTTTTTGGGGGAAAAACCC
   PRODUCT SIZE: 105, PAIR ANY COMPL: 25.00, PAIR 3' COMPL: 25.00

 4 LEFT PRIMER         28   25   67.77   40.00 16.00 16.00 CCTTTTTGGGGGAAAAATTTTTCCC
   RIGHT PRIMER       130   25   67.77   40.00 12.00  3.00 GAAAAATTTTTCCCCCAAAAAGGGG
   INTERNAL OLIGO      87   19   60.50   47.37  5.00  3.00 CCCTTTTTGGGGGAAAAAC
   PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     131     0     0     0     0     0     0     0     0     0     0     0     0   131
Right    131     0     0     0     0     0     0     0     0     0     0     0     0   131
Intl    1585     0     0     0     0     0     0     0     0     0     0     0     0  1585
Pair Stats:
considered 64, unacceptable product size 48, ok 16
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR poly_x_3

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 204
INCLUDED REGION SIZE: 204


    0 AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCC
   60 TTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGG
  120 GGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCC
  180 CCCTTTTTTTGGGGGGGGGGGGGG


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     155     0     0     0     0     0     0     0     0     0     0    57     0    98
Right    155     0     0     0     0     0     0     0     0     0     0    72     0    83
Intl     187     0     0     0     0     0     0     0     0     0     0   187     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_59

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 27,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_60

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

EXCLUDED REGIONS (start, len)*: 26,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN

XXXXXX excluded region

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     1     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_61

INPUT PROBLEM: Negative EXCLUDED_REGION length

PRIMER PICKING RESULTS FOR boundary_test_62

No mispriming library specified
Using 0-based sequence positions
WARNING: EXCLUDED_REGION outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 1,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_63

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

EXCLUDED REGIONS (start, len)*: 2,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..X                                                      ..

KEYS (in order of precedence):
...... vector sequence
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     1     0     0     0     0     0     0     0     0     0     0
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_64

INPUT PROBLEM: EXCLUDED_REGION beyond end of sequence

PRIMER PICKING RESULTS FOR boundary_test_65

No mispriming library specified
Using 0-based sequence positions
WARNING: EXCLUDED_REGION outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
EXCLUDED REGIONS (start, len)*: 58,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_66

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

EXCLUDED REGIONS (start, len)*: 56,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                      X..

KEYS (in order of precedence):
...... vector sequence
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     1     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_67

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

WARNING: EXCLUDED_REGION outside of INCLUDED_REGION

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

EXCLUDED REGIONS (start, len)*: 56,2

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                      X..

KEYS (in order of precedence):
...... vector sequence
XXXXXX excluded region


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       6     5     0     0     0     0     0     0     0     0     0     0     0     1
Right      6     5     0     1     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_68

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_69

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 26,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN

****** target

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       2     2     0     0     0     0     0     0     0     0     0     0     0     0
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_70

INPUT PROBLEM: Negative TARGET length

PRIMER PICKING RESULTS FOR boundary_test_71

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

WARNING: TARGET outside of INCLUDED_REGION

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

TARGETS (start, len)*: 1,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                       ..

KEYS (in order of precedence):
...... vector sequence
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, no target 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_72

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

TARGETS (start, len)*: 2,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..*                                                      ..

KEYS (in order of precedence):
...... vector sequence
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     1     0     0     0     0     0     0     0     0     0     0     0
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_73

INPUT PROBLEM: TARGET beyond end of sequence

PRIMER PICKING RESULTS FOR boundary_test_74

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

WARNING: TARGET outside of INCLUDED_REGION

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

TARGETS (start, len)*: 58,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                       ..

KEYS (in order of precedence):
...... vector sequence
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, no target 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_75

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

TARGETS (start, len)*: 56,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                      *..

KEYS (in order of precedence):
...... vector sequence
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      0     0     0     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_76

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

WARNING: TARGET outside of INCLUDED_REGION

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

TARGETS (start, len)*: 56,2

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                      *..

KEYS (in order of precedence):
...... vector sequence
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       6     5     0     0     0     0     0     0     0     0     0     0     0     1
Right      6     5     1     0     0     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_77

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>*    <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_78

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,5

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_79_1

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      6     5     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_80

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 31,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>    *<<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_81

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 32,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       6     5     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_82

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 32,0 0,1 58,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      * >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<< *

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     0     1
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_83

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

TARGETS (start, len)*: 0,1 58,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      *                                                         *

KEYS (in order of precedence):
****** target


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     0     1
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, no target 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_84

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 0,1 32,0

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      * >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       8     7     0     0     0     0     0     0     0     0     0     0     0     1
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_85

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 32,0 58,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<< *

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     0     1
Right      3     2     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_86

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   35   62.84   25.71 12.00 10.00 CACGTCATCTATATATATATCATCAAATTTGATTG
RIGHT PRIMER        76   35   61.36   25.71 10.00  8.00 GAATTGTTTGGAAATGACAGTATATATATATGAGT
SEQUENCE SIZE: 79
INCLUDED REGION SIZE: 79

PRODUCT SIZE: 75, PAIR ANY COMPL: 13.00, PAIR 3' COMPL: 8.00

    0 NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTG
        >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<

   60 TCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_71_2

No mispriming library specified
Using 0-based sequence positions
WARNING: TARGET outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 1,1 27,1

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>*    <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_72_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 56

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<< .

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     0     0     0     0     0     0     0     0     0     0     0     1
Right      3     2     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 3, unacceptable product size 2, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_73_2

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 56


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..                                                        .

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     0     0     0     0     0     0     0     0     0     0     0     1
Right      3     2     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 2, unacceptable product size 2, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_74_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 56

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<< .

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       3     2     0     0     0     0     0     0     0     0     0     0     0     1
Right      3     2     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_75_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       2     1     0     0     0     0     0     0     0     0     0     0     0     1
Right      2     1     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 2, unacceptable product size 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_76_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 56

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<< .

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       2     1     0     0     0     0     0     0     0     0     0     0     0     1
Right      2     1     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 2, unacceptable product size 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_77_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 86
INCLUDED REGION SIZE: 82

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<   

   60 CTCACTGTCATTTCCAAACAATTCNN
                              ..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER        83   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
   PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      28    27     0     0     0     0     0     0     0     0     0     0     0     1
Right     28    26     0     0     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 4, unacceptable product size 2, ok 2
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_78_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        83   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 86
INCLUDED REGION SIZE: 82

PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
      ..>>>>>>>>>>>>>>>>>>>>>>>>>                                <

   60 CTCACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
   PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      28    27     0     0     0     0     0     0     0     0     0     0     0     1
Right     28    26     0     0     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 4, unacceptable product size 2, ok 2
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_79_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 86
INCLUDED REGION SIZE: 82

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<   

   60 CTCACTGTCATTTCCAAACAATTCNN
                              ..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER        83   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
   PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      28    27     0     0     0     0     0     0     0     0     0     0     0     1
Right     28    26     0     0     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 3, unacceptable product size 1, ok 2
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_80_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        83   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 86
INCLUDED REGION SIZE: 82

PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA
      ..>>>>>>>>>>>>>>>>>>>>>>>>>                                <

   60 CTCACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
   PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      28    27     0     0     0     0     0     0     0     0     0     0     0     1
Right     28    26     0     0     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 3, unacceptable product size 1, ok 2
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_81_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.96   32.00  8.00  6.00 AATGTGATTGCATCAAATGTGATTG
RIGHT PRIMER        56   25   61.17   32.00  5.00  5.00 GAATTGTGGAAATGACAATCACATT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00

    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_82_2

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55


    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..                                                       ..

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, high any compl 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_83_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.96   32.00  8.00  6.00 AATGTGATTGCATCAAATGTGATTG
RIGHT PRIMER        56   25   61.17   32.00  5.00  5.00 GAATTGTGGAAATGACAATCACATT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00

    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_84_2

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55


    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..                                                       ..

KEYS (in order of precedence):
...... vector sequence


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, high end compl 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_85_2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.96   32.00  8.00  6.00 AATGTGATTGCATCAAATGTGATTG
RIGHT PRIMER        56   25   61.17   32.00  5.00  5.00 GAATTGTGGAAATGACAATCACATT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00

    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_86

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.96   32.00  8.00  6.00 AATGTGATTGCATCAAATGTGATTG
RIGHT PRIMER        56   25   61.17   32.00  5.00  5.00 GAATTGTGGAAATGACAATCACATT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
TARGETS (start, len)*: 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1

    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>*    <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_87

INPUT PROBLEM: Too many elements for tag SEQUENCE_TARGET

PRIMER PICKING RESULTS FOR boundary_test_88

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.96   32.00  8.00  6.00 AATGTGATTGCATCAAATGTGATTG
RIGHT PRIMER        56   25   61.17   32.00  5.00  5.00 GAATTGTGGAAATGACAATCACATT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 55

PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00
EXCLUDED REGIONS (start, len)*: 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1

    0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN
      ..>>>>>>>>>>>>>>>>>>>>>>>>>X    <<<<<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
XXXXXX excluded region
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_89

INPUT PROBLEM: Too many elements for tag SEQUENCE_EXCLUDED_REGION

PRIMER PICKING RESULTS FOR boundary_test_90

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN
        >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     0     1
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_91

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     9     0     0     0     0     0     0     0     0     0     0     1     0
Right     10     9     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_92

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          2   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
POSITION PENALTY, QUALITY: 0.000000, 2.731673
RIGHT PRIMER        56   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
POSITION PENALTY, QUALITY: 400.000000, 400.649600
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,1

    0 ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG
        >>>>>>>>>>>>>>>>>>>>>>>>>*    <<<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     2     0     0     0     0     0     0     0     0     0     0     0     3
Right      5     2     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 9, unacceptable product size 8, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_93

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   25   60.94   32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT
POSITION PENALTY, QUALITY: 200.000000, 200.942558
RIGHT PRIMER        54   25   58.09   32.00  4.00  2.00 ATTGTTTGGAAATGACAGTGAGTAT
POSITION PENALTY, QUALITY: 200.000000, 201.906454
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 27,1

    0 ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
      >>>>>>>>>>>>>>>>>>>>>>>>>  *  <<<<<<<<<<<<<<<<<<<<<<<<<    

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_94

No mispriming library specified
Using 0-based sequence positions
WARNING: Non-default inside penalty or outside penalty has no effect when number of targets is 0

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   25   60.94   32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT
POSITION PENALTY, QUALITY: 0.000000, 0.942558
RIGHT PRIMER        54   25   58.09   32.00  4.00  2.00 ATTGTTTGGAAATGACAGTGAGTAT
POSITION PENALTY, QUALITY: 0.000000, 1.906454
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00

    0 ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG
      >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<    

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_95

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   25   60.94   32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT
POSITION PENALTY, QUALITY: 0.000000, 0.942558
RIGHT PRIMER        54   25   58.56   36.00  4.00  4.00 ATTGTTTGGAAATGACAGTGAGTAC
POSITION PENALTY, QUALITY: 7000.000000, 7001.440912
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,10

    0 ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG
      >>>>>>>>>>>>>>>>>>>>>>>>>  ***(((((((<<<<<<<<<<<<<<<<<<    

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     0     0     0     0     0     0     0     0     0     0     0     0     5
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_96

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   25   59.25   28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
POSITION PENALTY, QUALITY: 2000.000000, 2000.754679
RIGHT PRIMER        58   25   64.16   40.00  4.00  3.00 CCGTATTGTTTGGAAATGACAGTGA
POSITION PENALTY, QUALITY: 3000.000000, 3004.155585
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 27,10

    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
          >>>>>>>>>>>>>>>>>>>>>>>))*****(((<<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR boundary_test_97

No mispriming library specified
Using 0-based sequence positions
WARNING: Non-default inside penalty or outside penalty has no effect when number of targets is 0

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   25   59.25   28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
POSITION PENALTY, QUALITY: 0.000000, 0.754679
RIGHT PRIMER        58   25   64.16   40.00  4.00  3.00 CCGTATTGTTTGGAAATGACAGTGA
POSITION PENALTY, QUALITY: 0.000000, 4.155585
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
          >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR test_098

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   25   59.25   28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
RIGHT PRIMER        58   25   64.16   40.00  4.00  3.00 CCGTATTGTTTGGAAATGACAGTGA
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
PRODUCT Tm: 62.4120, PRODUCT Tm - min(OLIGO Tm): 3.1667

    0 ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG
          >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR test_099

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, high product Tm 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR test_100

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   25   59.25   28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
RIGHT PRIMER        58   25   64.16   40.00  4.00  3.00 CCGTATTGTTTGGAAATGACAGTGA
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
          >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR test_101

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   25   59.25   28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
RIGHT PRIMER        58   25   64.16   40.00  4.00  3.00 CCGTATTGTTTGGAAATGACAGTGA
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
PRODUCT Tm: 63.9029, PRODUCT Tm - min(OLIGO Tm): 4.6576

    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
          >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR test_102

No mispriming library specified
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, low product Tm 1, ok 0
libprimer3 release 2.2.1


PRIMER PICKING RESULTS FOR test_103

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   25   59.25   28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT
RIGHT PRIMER        58   25   64.16   40.00  4.00  3.00 CCGTATTGTTTGGAAATGACAGTGA
SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59

PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

    0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG
          >>>>>>>>>>>>>>>>>>>>>>>>>     <<<<<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Pair Stats:
considered 5, unacceptable product size 4, ok 1
libprimer3 release 2.2.1


