PRIMER PICKING RESULTS FOR boundary_test_00

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
        >>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      35    33     0     0     0     0     0     0     0     0     0     0     0     2
Right     35    33     0     0     0     0     0     0     0     0     0     0     0     2
Intl      60    57     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 4, unacceptable product size 1, no internal oligo 2, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_01

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      30   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>>  ^^^^^^^^^^^^^^^^^^^^^^^^^   <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      30    28     0     0     0     0     0     0     0     0     0     0     0     2
Right     30    29     0     0     0     0     0     0     0     0     0     0     0     1
Intl      60    57     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 2, unacceptable product size 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_02

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      30   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>>  ^^^^^^^^^^^^^^^^^^^^^^^^^   <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Intl      60    57     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_05

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
RIGHT PRIMER        82   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      30   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

    1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^    <<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     3     0     0     0     0     0     0     0     0     0     0     0     2
Intl      60    57     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 4, unacceptable product size 3, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_11

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84


    1 NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACT
   61 CACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     1     0     0     0     0     0     0     0     0     0     0     0     4
Intl      60    59     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 8, unacceptable product size 6, no internal oligo 2, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_11

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   24   60.81   31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT
RIGHT PRIMER        82   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      30   25   63.22   34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

    1 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^    <<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          4   24   60.81   31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG
   RIGHT PRIMER        83   24   61.74   34.78  4.00  3.00 NGAATTGTTTGGAAATGACAGTGA
   INTERNAL OLIGO      30   25   63.22   34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
   PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Intl      60    52     0     0     0     0     0     0     0     0     0     0     0     8
Pair Stats:
considered 10, unacceptable product size 8, ok 2
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_12

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   24   60.81   31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT
RIGHT PRIMER        82   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      30   25   63.22   34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

    1 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^    <<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          4   24   60.81   31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG
   RIGHT PRIMER        83   24   61.74   34.78  4.00  3.00 NGAATTGTTTGGAAATGACAGTGA
   INTERNAL OLIGO      30   25   63.22   34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG
   PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     0     0     0     0     0     0     0     0     0     0     0     0     5
Intl      60    52     0     0     0     0     0     0     0     0     0     0     0     8
Pair Stats:
considered 10, unacceptable product size 8, ok 2
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_13

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   24   54.69    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAA
RIGHT PRIMER        82   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      32   25   56.20    0.00  0.00  0.00 TTTTTTTTTTTTTTTTTTTTTTTNN
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

    1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>     ^^^^^^^^^^^^^^^^^^^^^^^^^  <<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     3     0     0     0     0     0     0     0     0     0     0     0     2
Intl      60    23     0     0     0     0     0     0     0     0     0     0     0    37
Pair Stats:
considered 4, unacceptable product size 3, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_14

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   24   55.24    4.17  0.00  0.00 AAAAAAAAAAAAAAAAACAAAAAA
RIGHT PRIMER        82   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      30   25   56.20    0.00  0.00  0.00 TTTTTTTTTTTTTTTTTTTTTTTNN
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00

    1 NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACT
        >>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^    <<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     2     0     0     0     0     0     0     0     0     0     0     0     3
Right      5     3     0     0     0     0     0     0     0     0     0     0     0     2
Intl      60    25     0     0     0     0     0     0     0     0     0     0     0    35
Pair Stats:
considered 6, unacceptable product size 5, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_15

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   24   89.31  100.00  0.00  0.00 GGGGGGGGGGGGGGGGGGGGGGGG
RIGHT PRIMER        82   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      33   25   55.84    0.00  0.00  0.00 TTTTTTTTTTTTTTTTTTTTTTTTT
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00

    1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACT
        >>>>>>>>>>>>>>>>>>>>>>>>      ^^^^^^^^^^^^^^^^^^^^^^^^^ <<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     3     0     0     0     0     0     0     0     0     0     0     0     2
Right      5     2     0     0     0     0     0     0     0     0     0     0     0     3
Intl      60    25     0     0     0     0     0     0     0     0     0     0     0    35
Pair Stats:
considered 6, unacceptable product size 5, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_16

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 59
INCLUDED REGION SIZE: 59


    1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     8     0     0     0     0     0     0     0     0     0     0     0     2
Right     10     8     0     0     0     0     0     0     0     0     0     0     0     2
Intl      35     0     0     0     0     0     0     0     0     0     0     0     0    35
Pair Stats:
considered 4, no internal oligo 4, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_17

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84


    1 NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACT
   61 CACTGTCATTTCCAAACAATTCNN


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      35    31     0     0     0     0     0     0     0     0     0     0     0     4
Right     35    31     0     0     0     0     0     0     0     0     0     0     0     4
Intl      60     0     0     0     0    29     0     0     0     0     0     0     0    31
Pair Stats:
considered 16, unacceptable product size 4, no internal oligo 12, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_18

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 111
INCLUDED REGION SIZE: 111

INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 11,50

    1 NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGN
                xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

   61 NNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
                                                         

KEYS (in order of precedence):
xxxxxx excluded region for internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      62    53     0     0     0     0     0     0     0     0     0     0     0     9
Right     62    53     0     0     0     0     0     0     0     0     0     0     0     9
Intl      87    39     0    40     0     0     0     0     0     0     0     0     0     8
Pair Stats:
considered 81, unacceptable product size 49, no internal oligo 32, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_19

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   24   60.24   33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER       113   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      61   25   65.58   52.00  6.00  1.00 CCGTTAGGTGCTAGCCGTGTAGATT
SEQUENCE SIZE: 115
INCLUDED REGION SIZE: 115

PRODUCT SIZE: 110, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 11,50

    1 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNN
         >>>>>>>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

   61 CCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
      ^^^^^^^^^^^^^^^^^^^^^^^^^    <<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
   RIGHT PRIMER       113   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
   INTERNAL OLIGO      61   25   65.58   52.00  6.00  1.00 CCGTTAGGTGCTAGCCGTGTAGATT
   PRODUCT SIZE: 111, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER          4   24   60.24   33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER       112   24   58.98   33.33  4.00  3.00 AATTGTTTGGAAATGACAGTGAGT
   INTERNAL OLIGO      61   25   65.58   52.00  6.00  1.00 CCGTTAGGTGCTAGCCGTGTAGATT
   PRODUCT SIZE: 109, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
   RIGHT PRIMER       112   24   58.98   33.33  4.00  3.00 AATTGTTTGGAAATGACAGTGAGT
   INTERNAL OLIGO      61   25   65.58   52.00  6.00  1.00 CCGTTAGGTGCTAGCCGTGTAGATT
   PRODUCT SIZE: 110, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER         30   24   67.92   54.17  9.00  0.00 AAGCTAAGCTTGCCCGTATCCCCT
   RIGHT PRIMER       113   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
   INTERNAL OLIGO      61   25   65.58   52.00  6.00  1.00 CCGTTAGGTGCTAGCCGTGTAGATT
   PRODUCT SIZE: 84, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      66    57     0     0     0     0     0     0     0     0     0     0     0     9
Right     66    57     0     0     0     0     0     0     0     0     0     0     0     9
Intl      91    84     0     5     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 16, unacceptable product size 3, no internal oligo 8, ok 5
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_20

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   24   60.24   33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER       252   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      76   25   63.81   52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
SEQUENCE SIZE: 291
INCLUDED REGION SIZE: 250

PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 6,50 61,15

    1 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATA
      .. >>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx     

   61 CGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNN
      xxxxxxxxxxxxxxx^^^^^^^^^^^^^^^^^^^^^^^^^                    

  121 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  181 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATT
                                                      <<<<<<<<<<<<

  241 TCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
      <<<<<<<<<<<<.......................................

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
   RIGHT PRIMER       252   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
   INTERNAL OLIGO      76   25   63.81   52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
   PRODUCT SIZE: 250, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER         51   24   60.30   50.00  8.00  6.00 GGAGACGATACGGTAAGCTAGCTA
   RIGHT PRIMER       252   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
   INTERNAL OLIGO      76   25   63.81   52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
   PRODUCT SIZE: 202, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER          4   24   60.24   33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER       251   24   58.98   33.33  4.00  3.00 AATTGTTTGGAAATGACAGTGAGT
   INTERNAL OLIGO      76   25   63.81   52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
   PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
   RIGHT PRIMER       251   24   58.98   33.33  4.00  3.00 AATTGTTTGGAAATGACAGTGAGT
   INTERNAL OLIGO      76   25   63.81   52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG
   PRODUCT SIZE: 249, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     151    99     0     0     0     0     0     0     0     0     0     0     0    52
Right    151   148     0     0     0     0     0     0     0     0     0     0     0     3
Intl     226   175     0    47     0     0     0     0     0     0     0     0     0     4
Pair Stats:
considered 14, no internal oligo 8, ok 6
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_21

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          4   24   60.24   33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER       174   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
INTERNAL OLIGO      62   25   55.84    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
SEQUENCE SIZE: 176
INCLUDED REGION SIZE: 176

PRODUCT SIZE: 171, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

    1 NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
         >>>>>>>>>>>>>>>>>>>>>>>>                                 

   61 GAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
       ^^^^^^^^^^^^^^^^^^^^^^^^^                                  

  121 GGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
                                    <<<<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
   RIGHT PRIMER       174   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
   INTERNAL OLIGO      62   25   55.84    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
   PRODUCT SIZE: 172, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER          4   24   60.24   33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG
   RIGHT PRIMER       173   24   58.98   33.33  4.00  3.00 AATTGTTTGGAAATGACAGTGAGT
   INTERNAL OLIGO      62   25   55.84    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
   PRODUCT SIZE: 170, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER          3   24   60.24   33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT
   RIGHT PRIMER       173   24   58.98   33.33  4.00  3.00 AATTGTTTGGAAATGACAGTGAGT
   INTERNAL OLIGO      62   25   55.84    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
   PRODUCT SIZE: 171, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER         38   24   89.31  100.00  0.00  0.00 GGGGGGGGGGGGGGGGGGGGGGGG
   RIGHT PRIMER       174   24   59.91   37.50  4.00  1.00 GAATTGTTTGGAAATGACAGTGAG
   INTERNAL OLIGO      62   25   55.84    0.00  0.00  0.00 AAAAAAAAAAAAAAAAAAAAAAAAA
   PRODUCT SIZE: 137, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      77    27     0     0     0     0     0     0     0     0     0     0     0    50
Right     77    28     0     0     0     0     0     0     0     0     0     0     0    49
Intl     152    57     0     0     0     0     0     1    93     0     0     0     0     1
Pair Stats:
considered 323, unacceptable product size 223, no internal oligo 87, ok 13
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_22

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 84

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
        >>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       5     4     0     0     0     0     0     0     0     0     0     0     0     1
Right      5     4     0     0     0     0     0     0     0     0     0     0     0     1
Intl      60    57     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_23

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 2,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_24

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 3,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..x>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_25

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 1,3

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..x>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_26

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 30,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>  x^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_27

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 31,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..                            x                             

   61 CACTGTCATTTCCAAACAATTCNN
                            ..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, no internal oligo 1, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_28

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 55,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..                                                    x     

   61 CACTGTCATTTCCAAACAATTCNN
                            ..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, no internal oligo 1, ok 0
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_29

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 56,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^x <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_30

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<x..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_31

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 83,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<<..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     0     0     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_32

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,2

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<x..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_33

No mispriming library specified
No internal oligo mishyb library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          3   25   62.73   36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG
RIGHT PRIMER        82   25   60.65   36.00  4.00  3.00 GAATTGTTTGGAAATGACAGTGAGT
INTERNAL OLIGO      31   25   71.50   56.00 12.00  8.00 ACGTACGTACGTTGCAGTCGACGCA
SEQUENCE SIZE: 84
INCLUDED REGION SIZE: 80

PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1

    1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT
      ..>>>>>>>>>>>>>>>>>>>>>>>>>   ^^^^^^^^^^^^^^^^^^^^^^^^^  <<<

   61 CACTGTCATTTCCAAACAATTCNN
      <<<<<<<<<<<<<<<<<<<<<x..

KEYS (in order of precedence):
...... vector sequence
xxxxxx excluded region for internal oligo
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Intl      56    53     0     1     0     0     0     0     0     0     0     0     0     2
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR boundary_test_34

INPUT PROBLEM: Too many elements for tag SEQUENCE_INTERNAL_EXCLUDED_REGION

