SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 1 any right primer - OK
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=40-100
PRIMER_MIN_GC=0
PRIMER_MIN_TM=0
PRIMER_PICK_ANYWAY=0
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_MAX_POLY_X=100
PRIMER_MAX_SELF_ANY=100
PRIMER_MAX_SELF_END=100
PRIMER_FIRST_BASE_INDEX=1
PRIMER_MIN_SIZE=16
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAga
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=22.403937
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=13.461417
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAga
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,18
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=48.539
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=27.778
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=65
PRIMER_PAIR_0_PRODUCT_TM=62.0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 2 any left primer - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,17
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=25.992387
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=17.049867
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,17
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=45.950
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=29.412
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=65
PRIMER_PAIR_0_PRODUCT_TM=62.0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 3 any pair - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccA
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=29.017881
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=20.075362
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccA
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,16
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=43.925
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=25.000
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=65
PRIMER_PAIR_0_PRODUCT_TM=62.0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 4 constrained left and right primers - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=25.992387
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=17.049867
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,17
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=45.950
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=29.412
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=65
PRIMER_PAIR_0_PRODUCT_TM=62.0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 5 constrained left and right primers - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,10
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, not in any ok left region 1, ok 0
PRIMER_RIGHT_EXPLAIN=considered 1, not in any ok right region 1, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 6 constrained left and right primers - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,7 ; 1,25,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=25.992387
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=17.049867
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,17
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=45.950
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=29.412
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=65
PRIMER_PAIR_0_PRODUCT_TM=62.0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 7 contrained left and right primers - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,19,49,17 ; 1,20,50,16
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, not in any ok region 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 8 any left primer - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,16
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, not in any ok right region 1, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 9 any right primer - NOT OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,,
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, not in any ok left region 1, ok 0
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 10 any right primer, any left primer - OK
SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,, ; ,,49,17
SEQUENCE_PRIMER=cttgattcaatgaatctcaa
SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=25.992387
PRIMER_LEFT_0_PENALTY=8.942519
PRIMER_RIGHT_0_PENALTY=17.049867
PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa
PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg
PRIMER_LEFT_0=1,20
PRIMER_RIGHT_0=65,17
PRIMER_LEFT_0_TM=51.057
PRIMER_RIGHT_0_TM=45.950
PRIMER_LEFT_0_GC_PERCENT=30.000
PRIMER_RIGHT_0_GC_PERCENT=29.412
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=65
PRIMER_PAIR_0_PRODUCT_TM=62.0
=
