PRIMER PICKING RESULTS FOR thermod_default

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         11   20   59.70   55.00    0.00   0.00    0.00 GACTTCACTTTCCGACCCTG
RIGHT PRIMER       113   20   59.96   50.00    3.73   0.00    0.00 TGACGGTCATCATGGTGAGT
INTERNAL OLIGO      44   20   60.71   60.00    1.74   0.00   40.53 CACCCACGAGTGAAGACTCC
SEQUENCE SIZE: 178
INCLUDED REGION SIZE: 178

PRODUCT SIZE: 103, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA
                 >>>>>>>>>>>>>>>>>>>>             ^^^^^^^^^^^^^^^^

   60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGA
      ^^^^                              <<<<<<<<<<<<<<<<<<<<      

  120 GGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
                                                                

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         10   20   59.70   50.00    0.00   0.00    0.00 TGACTTCACTTTCCGACCCT
   RIGHT PRIMER       113   20   59.96   50.00    3.73   0.00    0.00 TGACGGTCATCATGGTGAGT
   INTERNAL OLIGO      44   20   60.71   60.00    1.74   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 104, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         11   20   59.70   55.00    0.00   0.00    0.00 GACTTCACTTTCCGACCCTG
   RIGHT PRIMER       114   20   60.11   55.00    3.73   0.00    0.00 CTGACGGTCATCATGGTGAG
   INTERNAL OLIGO      44   20   60.71   60.00    1.74   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 104, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         10   20   59.70   50.00    0.00   0.00    0.00 TGACTTCACTTTCCGACCCT
   RIGHT PRIMER       114   20   60.11   55.00    3.73   0.00    0.00 CTGACGGTCATCATGGTGAG
   INTERNAL OLIGO      44   20   60.71   60.00    1.74   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 105, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER          2   20   59.29   50.00    0.00   0.00    0.00 GCCGAGGATGACTTCACTTT
   RIGHT PRIMER       113   20   59.96   50.00    3.73   0.00    0.00 TGACGGTCATCATGGTGAGT
   INTERNAL OLIGO      44   20   60.71   60.00    1.74   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 112, PAIR ANY_TH COMPL: 7.99, PAIR 3'_TH COMPL: 3.92

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    1290     0     0     0     0    10     0    60  1039      0     0     0     0     0   181
Right   1290     0     0     0     0    32     0    38  1096      0     0     0     0     0   124
Intl    1565     0     0     0     0    32     0    60  1290      0     0     0     0     0   183
Pair Stats:
considered 25, unacceptable product size 20, ok 5
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR any_1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         17   20   60.01   50.00    6.12   0.00   42.83 CAAGCAATACGACCTGCTGA
RIGHT PRIMER       168   20   59.98   50.00    0.00   0.00   39.48 ATCGCAAGTGAGGATGAGCT
INTERNAL OLIGO     116   21   60.58   38.10    8.89   8.89   37.82 CGTGTGCAAAACATTTTCACA
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                       >>>>>>>>>>>>>>>>>>>>                       

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                                              ^^^^

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
      ^^^^^^^^^^^^^^^^^            <<<<<<<<<<<<<<<<<<<<           

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                                  

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
                                                                  

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         17   20   60.01   50.00    6.12   0.00   42.83 CAAGCAATACGACCTGCTGA
   RIGHT PRIMER       191   20   59.94   40.00    5.65   0.00   39.80 CAAAAGATTTTGGCGGGTAA
   INTERNAL OLIGO     149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   PRODUCT SIZE: 175, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         17   20   60.01   50.00    6.12   0.00   42.83 CAAGCAATACGACCTGCTGA
   RIGHT PRIMER       368   20   59.75   60.00    7.28   0.00    0.00 CCGTACGCACTATAGGAGCC
   INTERNAL OLIGO     149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   PRODUCT SIZE: 352, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   RIGHT PRIMER       368   20   59.75   60.00    7.28   0.00    0.00 CCGTACGCACTATAGGAGCC
   INTERNAL OLIGO     172   20   59.94   40.00    4.21   4.21   43.62 TTACCCGCCAAAATCTTTTG
   PRODUCT SIZE: 220, PAIR ANY_TH COMPL: 9.42, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         17   20   60.01   50.00    6.12   0.00   42.83 CAAGCAATACGACCTGCTGA
   RIGHT PRIMER       232   20   59.70   50.00    0.00   0.00    0.00 CACTAAAGCGGGATTTGAGG
   INTERNAL OLIGO     149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   PRODUCT SIZE: 216, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0   897  1543      0     0     0    22     0   935
Right   3397     0     0     0     0     0     0   839  1673      0     0     1    22     0   862
Intl    3672     0     0     0     0     0     0   953  1734      0     0    14    22     0   949
Pair Stats:
considered 58, unacceptable product size 51, ok 7
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR any_salt

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      0     0     1    22     0   183
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545      0     0    10    22     0   663
Pair Stats:
considered 18, unacceptable product size 11, high template mispriming score 1, ok 6
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR any_weight

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      0     0     1    22     0   183
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545      0     0    10    22     0   663
Pair Stats:
considered 20, unacceptable product size 11, high template mispriming score 3, ok 6
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR any_2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3146     0     0     0     0     0     0    52  2915     33     0     1    22     0   123
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3126     0     0     0     0     0     0   432  2140     83     0    10    22     0   439
Pair Stats:
considered 23, unacceptable product size 12, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR any_no_effect

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      0     0     1    22     0   183
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545      2     0    10    22     0   661
Pair Stats:
considered 18, unacceptable product size 11, high template mispriming score 1, ok 6
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR end_th1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      0     0     1    22     0   183
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545      2     0    10    22     0   661
Pair Stats:
considered 18, unacceptable product size 11, high template mispriming score 1, ok 6
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR end_th2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     1    22     0   111
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    10    22     0   376
Pair Stats:
considered 23, unacceptable product size 12, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR hairpin1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     8    22     0   104
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    10    22     0   376
Pair Stats:
considered 19, unacceptable product size 8, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR hairpin2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     1    22     0   111
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    10    22     0   376
Pair Stats:
considered 23, unacceptable product size 12, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR hairpin3

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     1    22     0   111
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    28    22     0   358
Pair Stats:
considered 23, unacceptable product size 12, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR template1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     1    22     0   111
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    28    22     0   358
Pair Stats:
considered 20, unacceptable product size 11, high template mispriming score 1, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR template2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     1    22     0   111
Right   3209     0     0     0     0     0     0    59  2966     24     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    28    22     0   358
Pair Stats:
considered 23, unacceptable product size 12, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR template2_and_repeat_library

Using mispriming library ../src/humrep_and_simple.txt
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin   rep seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 12.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 11.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin   rep seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 12.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 11.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 11.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 11.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 11.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 11.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin   sim     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0     1     0    22     0   111
Right   3209     0     0     0     0     0     0    59  2966     24     0     0     0    18     0   142
Intl    3030     0     0     0     0     0     0   432  2088    102     0    28     0    22     0   358
Pair Stats:
considered 23, unacceptable product size 12, high template mispriming score 3, ok 8
libprimer3 release 2.3.6


PRIMER PICKING RESULTS FOR template3

Using mispriming library ../src/humrep_and_simple.txt
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin   rep seq
LEFT PRIMER         10   18   61.54   33.33    0.00   0.00    0.00 10.00 TTCAATACAAGCAATACG
RIGHT PRIMER       122   18   61.01   44.44    0.00   0.00    0.00 11.00 GCACACGTTGTACAGATA
INTERNAL OLIGO      55   20   59.80   40.00    0.00   0.00    0.00       TCATCAAAGGAAAGTGAGCT
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 113, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                >>>>>>>>>>>>>>>>>>                           ^^^^^

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
      ^^^^^^^^^^^^^^^                              <<<<<<<<<<<<<<<

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
      <<<                                                         

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                                  

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
                                                                  

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin   rep seq

 1 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 11.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 141, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER          8   19   59.84   31.58    0.00   0.00    0.00 12.00 CATTCAATACAAGCAATAC
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 236, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         46   19   60.82   31.58    0.00   0.00    0.00 10.00 ATATTCAGTTCATCAAAGG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 198, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER        103   19   59.90   42.11    0.00   0.00    0.00 11.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       241   19   59.75   42.11    0.00   0.00    0.00 11.00 TCACTACTACACTAAAGCG
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 139, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin   sim     X  stab    ok
Left    3089     0     0     0     0     0     0    52  2863     40     0    25     3    22     0    84
Right   3209     0     0     0     0     0     0    59  2966     24     0    38     0    18     0   104
Intl    3030     0     0     0     0     0     0   432  2088    102     0    28     0    22     0   358
Pair Stats:
considered 8911, unacceptable product size 3799, high any compl 86, no internal oligo 571, high template mispriming score 119, ok 4336
libprimer3 release 2.3.6


