SEQUENCE_ID=negative first base index
[encoded data, 4379 characters]
PRIMER_EXPLAIN_FLAG=1
PRIMER_FIRST_BASE_INDEX=-200
PRIMER_NUM_RETURN=5
SEQUENCE_INCLUDED_REGION=-200,4361
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_MIN_THREE_PRIME_DISTANCE=0
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=25
PRIMER_OPT_GC_PERCENT=50
PRIMER_MIN_GC=45
PRIMER_MAX_GC=55
PRIMER_OPT_TM=60
PRIMER_MIN_TM=55
PRIMER_MAX_TM=80
PRIMER_GC_CLAMP=0
PRIMER_PAIR_MAX_DIFF_TM=40
PRIMER_MAX_POLY_X=5
PRIMER_MAX_SELF_ANY_TH=47
PRIMER_MAX_SELF_END_TH=47
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47
PRIMER_LIBERAL_BASE=0
PRIMER_MAX_NS_ACCEPTED=8
PRIMER_DNA_CONC=50
PRIMER_DNTP_CONC=0.6
PRIMER_SALT_MONOVALENT=50
PRIMER_SALT_DIVALENT=1.5
PRIMER_LEFT_EXPLAIN=considered 33850, GC content failed 23465, low tm 10, high tm 235, high end compl 5, long poly-x seq 66, ok 10069
PRIMER_RIGHT_EXPLAIN=considered 33850, GC content failed 23382, low tm 10, high tm 235, high any compl 1, high end compl 6, long poly-x seq 62, ok 10154
PRIMER_PAIR_EXPLAIN=considered 390, unacceptable product size 384, primer in pair overlaps a primer in a better pair 59, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.112355
PRIMER_LEFT_0_PENALTY=0.109144
PRIMER_RIGHT_0_PENALTY=0.003211
PRIMER_LEFT_0_SEQUENCE=ACGCTGTAGGTATCTCAGTT
PRIMER_RIGHT_0_SEQUENCE=CCTTCTAGTGTAGCCGTAGT
PRIMER_LEFT_0=2534,20
PRIMER_RIGHT_0=2774,20
PRIMER_LEFT_0_TM=60.109
PRIMER_RIGHT_0_TM=59.997
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=241
PRIMER_PAIR_0_PRODUCT_TM=88.9
PRIMER_PAIR_1_PENALTY=0.233305
PRIMER_LEFT_1_PENALTY=0.141811
PRIMER_RIGHT_1_PENALTY=0.091494
PRIMER_LEFT_1_SEQUENCE=AACTTGGTCTGACAGTTACC
PRIMER_RIGHT_1_SEQUENCE=CTACTTACTCTAGCTTCCCG
PRIMER_LEFT_1=3077,20
PRIMER_RIGHT_1=3367,20
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.909
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=291
PRIMER_PAIR_1_PRODUCT_TM=87.5
PRIMER_PAIR_2_PENALTY=0.354043
PRIMER_LEFT_2_PENALTY=0.278851
PRIMER_RIGHT_2_PENALTY=0.075193
PRIMER_LEFT_2_SEQUENCE=AGATAACTACGATACGGGAG
PRIMER_RIGHT_2_SEQUENCE=AACTGGCGAACTACTTACTC
PRIMER_LEFT_2=3175,20
PRIMER_RIGHT_2=3377,20
PRIMER_LEFT_2_TM=59.721
PRIMER_RIGHT_2_TM=59.925
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=203
PRIMER_PAIR_2_PRODUCT_TM=87.5
PRIMER_PAIR_3_PENALTY=0.447657
PRIMER_LEFT_3_PENALTY=0.338513
PRIMER_RIGHT_3_PENALTY=0.109144
PRIMER_LEFT_3_SEQUENCE=GACTATAAAGATACCAGGCG
PRIMER_RIGHT_3_SEQUENCE=AACTGAGATACCTACAGCGT
PRIMER_LEFT_3=2407,20
PRIMER_RIGHT_3=2553,20
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=147
PRIMER_PAIR_3_PRODUCT_TM=87.8
PRIMER_PAIR_4_PENALTY=0.568186
PRIMER_LEFT_4_PENALTY=0.163737
PRIMER_RIGHT_4_PENALTY=0.404449
PRIMER_LEFT_4_SEQUENCE=CTTATCCGGTAACTATCGTC
PRIMER_RIGHT_4_SEQUENCE=ACTGTCCTTCTAGTGTAGCC
PRIMER_LEFT_4=2621,20
PRIMER_RIGHT_4=2779,20
PRIMER_LEFT_4_TM=60.164
PRIMER_RIGHT_4_TM=59.596
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=159
PRIMER_PAIR_4_PRODUCT_TM=85.8
=
