PRIMER_TASK=generic
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=19
PRIMER_MAX_SIZE=21
PRIMER_NUM_RETURN=1
PRIMER_MAX_SELF_ANY_TH=47.00
PRIMER_MAX_SELF_END_TH=47.00
PRIMER_MAX_HAIRPIN_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00
PRIMER_PAIR_MAX_COMPL_END_TH=47.00
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt
SEQUENCE_ID=pick_detection_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_detection_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_left_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_left_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_left_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_right_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_right_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_right_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_hyb_probe_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_hyb_probe_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_hyb_probe_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_primer_list
PRIMER_NUM_RETURN=10000
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_primer_list_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_pick_primer_list_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_primer_list
PRIMER_PRODUCT_SIZE_RANGE=75-200
P3_FILE_FLAG=1
SEQUENCE_ID=pick_primer_list_and_file_flag_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
P3_FILE_FLAG=1
SEQUENCE_ID=pick_primer_list_and_file_flag_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_primer_list
PRIMER_PRODUCT_SIZE_RANGE=75-200
P3_FILE_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=4
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=4
SEQUENCE_ID=pick_primer_list_and_file_flag_th_3
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
P3_FILE_FLAG=1
SEQUENCE_ID=pick_primer_list_and_file_flag_th_4
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_NUM_RETURN=1
P3_FILE_FLAG=0
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_sequencing_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_ID=pick_sequencing_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
PRIMER_PICK_RIGHT_PRIMER=1
SEQUENCE_ID=pick_sequencing_primers_th_3
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
=
PRIMER_TASK=check_primers
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=check_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
SEQUENCE_PRIMER=GACATGACACTGACGATGCA
SEQUENCE_PRIMER_REVCOMP=TAGCTATCGATTTGGGTCGG
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=check_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_PRIMER=TGACCTGATAGAAACGCCAA
SEQUENCE_PRIMER_REVCOMP=TGCTTTGCGTTTTGGAATAG
=
PRIMER_TASK=pick_discriminative_primers
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_disc_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_PICK_ANYWAY=1
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_disc_primer_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_TARGET=37,21
=
PRIMER_TASK=pick_cloning_primers
PRIMER_PRODUCT_SIZE_RANGE=200-300
SEQUENCE_ID=pick_disc_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=2,100
=
PRIMER_PRODUCT_SIZE_RANGE=200-300
SEQUENCE_ID=pick_disc_primer_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_TARGET=2,120
=
