PRIMER_OPT_SIZE=20
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_MAX_POLY_X=100
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
PRIMER_MAX_GC=80.0
PRIMER_MAX_SIZE=25
PRIMER_MAX_TM=63.0
PRIMER_MIN_SIZE=18
PRIMER_MIN_GC=20
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=6
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=6
SEQUENCE_ID=01.RHAP33FA1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=20,315
SEQUENCE_TARGET=81,30
P3_COMMENT=CL is GCCTTCATTAGAAAGTGAAAGTAAA
P3_COMMENT=CR is GGCAAAAGGACAGAGGATGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.733854
PRIMER_LEFT_0_PENALTY=7.538496
PRIMER_RIGHT_0_PENALTY=0.195358
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_0_SEQUENCE=GGCAAAAGGACAGAGGATGA
PRIMER_LEFT_0=20,25
PRIMER_RIGHT_0=177,20
PRIMER_LEFT_0_TM=57.462
PRIMER_RIGHT_0_TM=60.195
PRIMER_LEFT_0_GC_PERCENT=32.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=158
PRIMER_PAIR_0_PRODUCT_TM=66.7
PRIMER_PAIR_1_PENALTY=7.868046
PRIMER_LEFT_1_PENALTY=7.538496
PRIMER_RIGHT_1_PENALTY=0.329550
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
PRIMER_LEFT_1=20,25
PRIMER_RIGHT_1=183,20
PRIMER_LEFT_1_TM=57.462
PRIMER_RIGHT_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=164
PRIMER_PAIR_1_PRODUCT_TM=67.0
PRIMER_PAIR_2_PENALTY=7.904946
PRIMER_LEFT_2_PENALTY=7.538496
PRIMER_RIGHT_2_PENALTY=0.366450
PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_2_SEQUENCE=TCTTGGGCAAAAGGACAGAG
PRIMER_LEFT_2=20,25
PRIMER_RIGHT_2=182,20
PRIMER_LEFT_2_TM=57.462
PRIMER_RIGHT_2_TM=60.366
PRIMER_LEFT_2_GC_PERCENT=32.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=163
PRIMER_PAIR_2_PRODUCT_TM=67.0
PRIMER_PAIR_3_PENALTY=8.845017
PRIMER_LEFT_3_PENALTY=7.538496
PRIMER_RIGHT_3_PENALTY=1.306521
PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_3_SEQUENCE=TATCTTGGGCAAAAGGACAGA
PRIMER_LEFT_3=20,25
PRIMER_RIGHT_3=184,21
PRIMER_LEFT_3_TM=57.462
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=165
PRIMER_PAIR_3_PRODUCT_TM=67.0
PRIMER_PAIR_4_PENALTY=9.153890
PRIMER_LEFT_4_PENALTY=7.538496
PRIMER_RIGHT_4_PENALTY=1.615394
PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_4_SEQUENCE=ATCTTGGGCAAAAGGACAGAG
PRIMER_LEFT_4=20,25
PRIMER_RIGHT_4=183,21
PRIMER_LEFT_4_TM=57.462
PRIMER_RIGHT_4_TM=60.615
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=164
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=01.RHAP34FA1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
SEQUENCE_INCLUDED_REGION=19,296
SEQUENCE_TARGET=134,15
SEQUENCE_TARGET=213,16
SEQUENCE_TARGET=235,16
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=03.RHAP34FA3.seq
SEQUENCE_TEMPLATE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
SEQUENCE_INCLUDED_REGION=0,278
SEQUENCE_TARGET=1,28
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=03.RHAP34FE3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
SEQUENCE_INCLUDED_REGION=17,284
SEQUENCE_TARGET=27,60
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=04.RHAP33FE4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
SEQUENCE_INCLUDED_REGION=18,367
SEQUENCE_TARGET=146,46
SEQUENCE_TARGET=199,22
SEQUENCE_TARGET=286,13
SEQUENCE_TARGET=300,15
P3_COMMENT=CL is TGAGAAGGGAGGAGTAAGTACTGG
P3_COMMENT=CR is CCTATGCCTATACACCACACACA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.353468
PRIMER_LEFT_0_PENALTY=4.174047
PRIMER_RIGHT_0_PENALTY=3.179421
PRIMER_LEFT_0_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
PRIMER_RIGHT_0_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT_0=97,24
PRIMER_RIGHT_0=270,23
PRIMER_LEFT_0_TM=60.174
PRIMER_RIGHT_0_TM=59.821
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=67.3
PRIMER_PAIR_1_PENALTY=7.844268
PRIMER_LEFT_1_PENALTY=4.664847
PRIMER_RIGHT_1_PENALTY=3.179421
PRIMER_LEFT_1_SEQUENCE=GAAGGGAGGAGTAAGTACTGGAGA
PRIMER_RIGHT_1_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT_1=100,24
PRIMER_RIGHT_1=270,23
PRIMER_LEFT_1_TM=59.335
PRIMER_RIGHT_1_TM=59.821
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=171
PRIMER_PAIR_1_PRODUCT_TM=67.2
PRIMER_PAIR_2_PENALTY=7.844268
PRIMER_LEFT_2_PENALTY=4.664847
PRIMER_RIGHT_2_PENALTY=3.179421
PRIMER_LEFT_2_SEQUENCE=GAGAAGGGAGGAGTAAGTACTGGA
PRIMER_RIGHT_2_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT_2=98,24
PRIMER_RIGHT_2=270,23
PRIMER_LEFT_2_TM=59.335
PRIMER_RIGHT_2_TM=59.821
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=173
PRIMER_PAIR_2_PRODUCT_TM=67.3
PRIMER_PAIR_3_PENALTY=8.129938
PRIMER_LEFT_3_PENALTY=4.950517
PRIMER_RIGHT_3_PENALTY=3.179421
PRIMER_LEFT_3_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGA
PRIMER_RIGHT_3_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT_3=103,23
PRIMER_RIGHT_3=270,23
PRIMER_LEFT_3_TM=58.049
PRIMER_RIGHT_3_TM=59.821
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=168
PRIMER_PAIR_3_PRODUCT_TM=67.1
PRIMER_PAIR_4_PENALTY=8.185611
PRIMER_LEFT_4_PENALTY=5.006190
PRIMER_RIGHT_4_PENALTY=3.179421
PRIMER_LEFT_4_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGAG
PRIMER_RIGHT_4_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT_4=103,24
PRIMER_RIGHT_4=270,23
PRIMER_LEFT_4_TM=58.994
PRIMER_RIGHT_4_TM=59.821
PRIMER_LEFT_4_GC_PERCENT=54.167
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=168
PRIMER_PAIR_4_PRODUCT_TM=67.1
=
SEQUENCE_ID=05.RHAP33FA5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
SEQUENCE_INCLUDED_REGION=10,369
SEQUENCE_TARGET=30,44
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=05.RHAP33FE5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=14,306
SEQUENCE_TARGET=176,34
P3_COMMENT=CL is CATGTTCGTGCAAACACTCA
P3_COMMENT=CR is AGACCCTGTCTCCAAAAGCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.858517
PRIMER_LEFT_0_PENALTY=0.702456
PRIMER_RIGHT_0_PENALTY=0.156061
PRIMER_LEFT_0_SEQUENCE=CATGTTCGTGCAAACACTCA
PRIMER_RIGHT_0_SEQUENCE=AGACCCTGTCTCCAAAAGCA
PRIMER_LEFT_0=129,20
PRIMER_RIGHT_0=302,20
PRIMER_LEFT_0_TM=59.298
PRIMER_RIGHT_0_TM=59.844
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=66.1
PRIMER_PAIR_1_PENALTY=0.922007
PRIMER_LEFT_1_PENALTY=0.702456
PRIMER_RIGHT_1_PENALTY=0.219551
PRIMER_LEFT_1_SEQUENCE=CATGTTCGTGCAAACACTCA
PRIMER_RIGHT_1_SEQUENCE=CCCTGTCTCCAAAAGCAAAA
PRIMER_LEFT_1=129,20
PRIMER_RIGHT_1=299,20
PRIMER_LEFT_1_TM=59.298
PRIMER_RIGHT_1_TM=60.220
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=171
PRIMER_PAIR_1_PRODUCT_TM=66.0
PRIMER_PAIR_2_PENALTY=0.934756
PRIMER_LEFT_2_PENALTY=0.702456
PRIMER_RIGHT_2_PENALTY=0.232299
PRIMER_LEFT_2_SEQUENCE=CATGTTCGTGCAAACACTCA
PRIMER_RIGHT_2_SEQUENCE=GACCCTGTCTCCAAAAGCAA
PRIMER_LEFT_2=129,20
PRIMER_RIGHT_2=301,20
PRIMER_LEFT_2_TM=59.298
PRIMER_RIGHT_2_TM=60.232
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=173
PRIMER_PAIR_2_PRODUCT_TM=66.2
PRIMER_PAIR_3_PENALTY=0.991260
PRIMER_LEFT_3_PENALTY=0.702456
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=CATGTTCGTGCAAACACTCA
PRIMER_RIGHT_3_SEQUENCE=ACCCTGTCTCCAAAAGCAAA
PRIMER_LEFT_3=129,20
PRIMER_RIGHT_3=300,20
PRIMER_LEFT_3_TM=59.298
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=172
PRIMER_PAIR_3_PRODUCT_TM=65.9
PRIMER_PAIR_4_PENALTY=1.268733
PRIMER_LEFT_4_PENALTY=1.112672
PRIMER_RIGHT_4_PENALTY=0.156061
PRIMER_LEFT_4_SEQUENCE=TGTTCGTGCAAACACTCAAA
PRIMER_RIGHT_4_SEQUENCE=AGACCCTGTCTCCAAAAGCA
PRIMER_LEFT_4=131,20
PRIMER_RIGHT_4=302,20
PRIMER_LEFT_4_TM=58.887
PRIMER_RIGHT_4_TM=59.844
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=65.9
=
SEQUENCE_ID=05.RHAP34FA5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
SEQUENCE_INCLUDED_REGION=9,297
SEQUENCE_TARGET=125,36
SEQUENCE_TARGET=162,24
SEQUENCE_TARGET=191,16
SEQUENCE_TARGET=214,14
P3_COMMENT=CL is GCGGCCGACATCTACTACTA
P3_COMMENT=CR is TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=9.884342
PRIMER_LEFT_0_PENALTY=1.973952
PRIMER_RIGHT_0_PENALTY=7.910390
PRIMER_LEFT_0_SEQUENCE=GCGGCCGACATCTACTACTA
PRIMER_RIGHT_0_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_0=103,20
PRIMER_RIGHT_0=268,25
PRIMER_LEFT_0_TM=58.026
PRIMER_RIGHT_0_TM=57.090
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=44.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=4.7000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=166
PRIMER_PAIR_0_PRODUCT_TM=65.2
PRIMER_PAIR_1_PENALTY=10.012256
PRIMER_LEFT_1_PENALTY=2.101866
PRIMER_RIGHT_1_PENALTY=7.910390
PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_1=103,21
PRIMER_RIGHT_1=268,25
PRIMER_LEFT_1_TM=58.898
PRIMER_RIGHT_1_TM=57.090
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=166
PRIMER_PAIR_1_PRODUCT_TM=65.2
PRIMER_PAIR_2_PENALTY=10.079221
PRIMER_LEFT_2_PENALTY=2.168831
PRIMER_RIGHT_2_PENALTY=7.910390
PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=268,25
PRIMER_LEFT_2_TM=59.831
PRIMER_RIGHT_2_TM=57.090
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=44.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=65.2
PRIMER_PAIR_3_PENALTY=10.514211
PRIMER_LEFT_3_PENALTY=2.603822
PRIMER_RIGHT_3_PENALTY=7.910390
PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_3=102,20
PRIMER_RIGHT_3=268,25
PRIMER_LEFT_3_TM=62.604
PRIMER_RIGHT_3_TM=57.090
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=44.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=65.4
PRIMER_PAIR_4_PENALTY=10.656616
PRIMER_LEFT_4_PENALTY=2.746227
PRIMER_RIGHT_4_PENALTY=7.910390
PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_4=102,19
PRIMER_RIGHT_4=268,25
PRIMER_LEFT_4_TM=61.746
PRIMER_RIGHT_4_TM=57.090
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=44.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=167
PRIMER_PAIR_4_PRODUCT_TM=65.4
=
SEQUENCE_ID=06.RHAP34FE6.seq
SEQUENCE_TEMPLATE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
SEQUENCE_INCLUDED_REGION=8,287
SEQUENCE_TARGET=61,68
P3_COMMENT=CL is CAACCCAAAAATACAGACGGA
P3_COMMENT=CR is CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.096487
PRIMER_LEFT_0_PENALTY=1.153194
PRIMER_RIGHT_0_PENALTY=0.943292
PRIMER_LEFT_0_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_0_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_0=9,21
PRIMER_RIGHT_0=148,20
PRIMER_LEFT_0_TM=59.847
PRIMER_RIGHT_0_TM=60.943
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=65.6
PRIMER_PAIR_1_PENALTY=2.623573
PRIMER_LEFT_1_PENALTY=1.153194
PRIMER_RIGHT_1_PENALTY=1.470379
PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=148,19
PRIMER_LEFT_1_TM=59.847
PRIMER_RIGHT_1_TM=59.530
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=140
PRIMER_PAIR_1_PRODUCT_TM=65.6
PRIMER_PAIR_2_PENALTY=2.943089
PRIMER_LEFT_2_PENALTY=1.999797
PRIMER_RIGHT_2_PENALTY=0.943292
PRIMER_LEFT_2_SEQUENCE=CAACCCAAAAATACAGACGG
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_2=9,20
PRIMER_RIGHT_2=148,20
PRIMER_LEFT_2_TM=58.000
PRIMER_RIGHT_2_TM=60.943
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=140
PRIMER_PAIR_2_PRODUCT_TM=65.6
PRIMER_PAIR_3_PENALTY=2.943089
PRIMER_LEFT_3_PENALTY=1.999797
PRIMER_RIGHT_3_PENALTY=0.943292
PRIMER_LEFT_3_SEQUENCE=CCAACCCAAAAATACAGACG
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_3=8,20
PRIMER_RIGHT_3=148,20
PRIMER_LEFT_3_TM=58.000
PRIMER_RIGHT_3_TM=60.943
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=141
PRIMER_PAIR_3_PRODUCT_TM=65.8
PRIMER_PAIR_4_PENALTY=3.406904
PRIMER_LEFT_4_PENALTY=2.463612
PRIMER_RIGHT_4_PENALTY=0.943292
PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=148,20
PRIMER_LEFT_4_TM=61.464
PRIMER_RIGHT_4_TM=60.943
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=141
PRIMER_PAIR_4_PRODUCT_TM=65.8
=
SEQUENCE_ID=07.RHAP34FE7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
SEQUENCE_INCLUDED_REGION=18,283
SEQUENCE_TARGET=115,29
SEQUENCE_TARGET=145,20
SEQUENCE_TARGET=166,24
P3_COMMENT=CL is TGGGTACAAACTGACAGGCA
P3_COMMENT=CR is TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.484594
PRIMER_LEFT_0_PENALTY=0.151542
PRIMER_RIGHT_0_PENALTY=0.333053
PRIMER_LEFT_0_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_0_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=238,20
PRIMER_LEFT_0_TM=60.152
PRIMER_RIGHT_0_TM=60.333
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=163
PRIMER_PAIR_0_PRODUCT_TM=67.3
PRIMER_PAIR_1_PENALTY=0.681190
PRIMER_LEFT_1_PENALTY=0.151542
PRIMER_RIGHT_1_PENALTY=0.529649
PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_1=76,20
PRIMER_RIGHT_1=235,20
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=59.470
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=160
PRIMER_PAIR_1_PRODUCT_TM=67.2
PRIMER_PAIR_2_PENALTY=0.789733
PRIMER_LEFT_2_PENALTY=0.151542
PRIMER_RIGHT_2_PENALTY=0.638191
PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_2=76,20
PRIMER_RIGHT_2=225,20
PRIMER_LEFT_2_TM=60.152
PRIMER_RIGHT_2_TM=60.638
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_2_PRODUCT_TM=66.0
PRIMER_PAIR_3_PENALTY=0.828489
PRIMER_LEFT_3_PENALTY=0.151542
PRIMER_RIGHT_3_PENALTY=0.676947
PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_3=76,20
PRIMER_RIGHT_3=237,20
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=59.323
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=162
PRIMER_PAIR_3_PRODUCT_TM=67.3
PRIMER_PAIR_4_PENALTY=0.837797
PRIMER_LEFT_4_PENALTY=0.151542
PRIMER_RIGHT_4_PENALTY=0.686256
PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_4_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_4=76,20
PRIMER_RIGHT_4=232,20
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=59.314
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=157
PRIMER_PAIR_4_PRODUCT_TM=66.8
=
SEQUENCE_ID=08.RHAP34FE8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
SEQUENCE_INCLUDED_REGION=20,281
SEQUENCE_TARGET=119,62
P3_COMMENT=CL is TTTCCCCTTAGGACCAAGGT
P3_COMMENT=CR is CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.218990
PRIMER_LEFT_0_PENALTY=0.201280
PRIMER_RIGHT_0_PENALTY=0.017711
PRIMER_LEFT_0_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_0_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_0=53,20
PRIMER_RIGHT_0=220,20
PRIMER_LEFT_0_TM=59.799
PRIMER_RIGHT_0_TM=59.982
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=168
PRIMER_PAIR_0_PRODUCT_TM=68.8
PRIMER_PAIR_1_PENALTY=0.267700
PRIMER_LEFT_1_PENALTY=0.249989
PRIMER_RIGHT_1_PENALTY=0.017711
PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_1=83,20
PRIMER_RIGHT_1=220,20
PRIMER_LEFT_1_TM=59.750
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=66.5
PRIMER_PAIR_2_PENALTY=0.357340
PRIMER_LEFT_2_PENALTY=0.201280
PRIMER_RIGHT_2_PENALTY=0.156061
PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_2=53,20
PRIMER_RIGHT_2=218,20
PRIMER_LEFT_2_TM=59.799
PRIMER_RIGHT_2_TM=59.844
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=68.6
PRIMER_PAIR_3_PENALTY=0.406050
PRIMER_LEFT_3_PENALTY=0.249989
PRIMER_RIGHT_3_PENALTY=0.156061
PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_3=83,20
PRIMER_RIGHT_3=218,20
PRIMER_LEFT_3_TM=59.750
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=136
PRIMER_PAIR_3_PRODUCT_TM=66.3
PRIMER_PAIR_4_PENALTY=0.440598
PRIMER_LEFT_4_PENALTY=0.422888
PRIMER_RIGHT_4_PENALTY=0.017711
PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_4=49,20
PRIMER_RIGHT_4=220,20
PRIMER_LEFT_4_TM=60.423
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=69.0
=
SEQUENCE_ID=09.RHAP33FA9.seq
SEQUENCE_TEMPLATE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
SEQUENCE_INCLUDED_REGION=0,371
SEQUENCE_TARGET=151,18
SEQUENCE_TARGET=192,53
SEQUENCE_TARGET=248,16
P3_COMMENT=CL is TGAGACATCCTGTTACCATGTAGG
P3_COMMENT=CR is CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.299756
PRIMER_LEFT_0_PENALTY=4.294975
PRIMER_RIGHT_0_PENALTY=4.004782
PRIMER_LEFT_0_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
PRIMER_RIGHT_0_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_0=19,24
PRIMER_RIGHT_0=191,18
PRIMER_LEFT_0_TM=60.295
PRIMER_RIGHT_0_TM=62.005
PRIMER_LEFT_0_GC_PERCENT=45.833
PRIMER_RIGHT_0_GC_PERCENT=72.222
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=173
PRIMER_PAIR_0_PRODUCT_TM=72.1
PRIMER_PAIR_1_PENALTY=8.306042
PRIMER_LEFT_1_PENALTY=4.301260
PRIMER_RIGHT_1_PENALTY=4.004782
PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_1=67,24
PRIMER_RIGHT_1=191,18
PRIMER_LEFT_1_TM=60.301
PRIMER_RIGHT_1_TM=62.005
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=72.222
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_1_PRODUCT_TM=70.5
PRIMER_PAIR_2_PENALTY=8.589410
PRIMER_LEFT_2_PENALTY=4.584629
PRIMER_RIGHT_2_PENALTY=4.004782
PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_2=20,24
PRIMER_RIGHT_2=191,18
PRIMER_LEFT_2_TM=59.415
PRIMER_RIGHT_2_TM=62.005
PRIMER_LEFT_2_GC_PERCENT=45.833
PRIMER_RIGHT_2_GC_PERCENT=72.222
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=172
PRIMER_PAIR_2_PRODUCT_TM=72.1
PRIMER_PAIR_3_PENALTY=8.956691
PRIMER_LEFT_3_PENALTY=4.951910
PRIMER_RIGHT_3_PENALTY=4.004782
PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=191,18
PRIMER_LEFT_3_TM=57.048
PRIMER_RIGHT_3_TM=62.005
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=72.222
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=72.0
PRIMER_PAIR_4_PENALTY=9.135884
PRIMER_LEFT_4_PENALTY=5.131103
PRIMER_RIGHT_4_PENALTY=4.004782
PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_4=48,23
PRIMER_RIGHT_4=191,18
PRIMER_LEFT_4_TM=57.869
PRIMER_RIGHT_4_TM=62.005
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=72.222
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=144
PRIMER_PAIR_4_PRODUCT_TM=71.1
=
SEQUENCE_ID=09.RHAP34FA9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
SEQUENCE_INCLUDED_REGION=20,280
SEQUENCE_TARGET=44,42
P3_COMMENT=CL is TGATCAGGATTTCAGTGCTAATAT
P3_COMMENT=CR is TTCCTCACAGAGGGAACAGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.200927
PRIMER_LEFT_0_PENALTY=6.971746
PRIMER_RIGHT_0_PENALTY=0.229181
PRIMER_LEFT_0_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_0_SEQUENCE=TTCCTCACAGAGGGAACAGG
PRIMER_LEFT_0=20,24
PRIMER_RIGHT_0=166,20
PRIMER_LEFT_0_TM=57.028
PRIMER_RIGHT_0_TM=60.229
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=147
PRIMER_PAIR_0_PRODUCT_TM=69.2
PRIMER_PAIR_1_PENALTY=7.687872
PRIMER_LEFT_1_PENALTY=6.971746
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
PRIMER_LEFT_1=20,24
PRIMER_RIGHT_1=150,20
PRIMER_LEFT_1_TM=57.028
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=131
PRIMER_PAIR_1_PRODUCT_TM=67.8
PRIMER_PAIR_2_PENALTY=7.830958
PRIMER_LEFT_2_PENALTY=6.971746
PRIMER_RIGHT_2_PENALTY=0.859212
PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
PRIMER_LEFT_2=20,24
PRIMER_RIGHT_2=151,20
PRIMER_LEFT_2_TM=57.028
PRIMER_RIGHT_2_TM=59.141
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=132
PRIMER_PAIR_2_PRODUCT_TM=67.8
PRIMER_PAIR_3_PENALTY=7.830958
PRIMER_LEFT_3_PENALTY=6.971746
PRIMER_RIGHT_3_PENALTY=0.859212
PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
PRIMER_LEFT_3=20,24
PRIMER_RIGHT_3=153,20
PRIMER_LEFT_3_TM=57.028
PRIMER_RIGHT_3_TM=59.141
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=134
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=8.039099
PRIMER_LEFT_4_PENALTY=6.971746
PRIMER_RIGHT_4_PENALTY=1.067353
PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
PRIMER_LEFT_4=20,24
PRIMER_RIGHT_4=147,21
PRIMER_LEFT_4_TM=57.028
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=128
PRIMER_PAIR_4_PRODUCT_TM=67.4
=
SEQUENCE_ID=10.RHAP34FA10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=12,236
SEQUENCE_TARGET=155,32
P3_COMMENT=CL is CCTGCAATGGGATGAATACA
P3_COMMENT=CR is CAAGGACAGAACTGAACTGGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.750634
PRIMER_LEFT_0_PENALTY=0.649665
PRIMER_RIGHT_0_PENALTY=1.100969
PRIMER_LEFT_0_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_0_SEQUENCE=CAAGGACAGAACTGAACTGGC
PRIMER_LEFT_0=101,20
PRIMER_RIGHT_0=229,21
PRIMER_LEFT_0_TM=59.350
PRIMER_RIGHT_0_TM=59.899
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=129
PRIMER_PAIR_0_PRODUCT_TM=66.1
PRIMER_PAIR_1_PENALTY=1.750634
PRIMER_LEFT_1_PENALTY=0.649665
PRIMER_RIGHT_1_PENALTY=1.100969
PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
PRIMER_LEFT_1=101,20
PRIMER_RIGHT_1=230,21
PRIMER_LEFT_1_TM=59.350
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=130
PRIMER_PAIR_1_PRODUCT_TM=66.3
PRIMER_PAIR_2_PENALTY=2.364169
PRIMER_LEFT_2_PENALTY=1.263200
PRIMER_RIGHT_2_PENALTY=1.100969
PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
PRIMER_LEFT_2=96,21
PRIMER_RIGHT_2=229,21
PRIMER_LEFT_2_TM=59.737
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=134
PRIMER_PAIR_2_PRODUCT_TM=66.1
PRIMER_PAIR_3_PENALTY=2.364169
PRIMER_LEFT_3_PENALTY=1.263200
PRIMER_RIGHT_3_PENALTY=1.100969
PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
PRIMER_LEFT_3=96,21
PRIMER_RIGHT_3=230,21
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.899
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=135
PRIMER_PAIR_3_PRODUCT_TM=66.4
PRIMER_PAIR_4_PENALTY=2.578428
PRIMER_LEFT_4_PENALTY=0.649665
PRIMER_RIGHT_4_PENALTY=1.928763
PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
PRIMER_LEFT_4=101,20
PRIMER_RIGHT_4=231,21
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=59.071
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=131
PRIMER_PAIR_4_PRODUCT_TM=66.3
=
SEQUENCE_ID=13.RHAP34FB1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,244
SEQUENCE_TARGET=22,16
SEQUENCE_TARGET=57,34
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=14.RHAP34FB2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
SEQUENCE_INCLUDED_REGION=13,303
SEQUENCE_TARGET=177,33
SEQUENCE_TARGET=254,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=14.RHAP34FF2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=20,248
SEQUENCE_TARGET=141,44
P3_COMMENT=CL is GGAGGCAGCAATGACATTTT
P3_COMMENT=CR is TACACACATTCGTACACGCG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.855316
PRIMER_LEFT_0_PENALTY=0.080962
PRIMER_RIGHT_0_PENALTY=0.774354
PRIMER_LEFT_0_SEQUENCE=GGAGGCAGCAATGACATTTT
PRIMER_RIGHT_0_SEQUENCE=TACACACATTCGTACACGCG
PRIMER_LEFT_0=73,20
PRIMER_RIGHT_0=204,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=59.226
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=11.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=67.8
PRIMER_PAIR_1_PENALTY=0.930642
PRIMER_LEFT_1_PENALTY=0.156287
PRIMER_RIGHT_1_PENALTY=0.774354
PRIMER_LEFT_1_SEQUENCE=AACCTTCAAGGCGTGTGTTC
PRIMER_RIGHT_1_SEQUENCE=TACACACATTCGTACACGCG
PRIMER_LEFT_1=34,20
PRIMER_RIGHT_1=204,20
PRIMER_LEFT_1_TM=60.156
PRIMER_RIGHT_1_TM=59.226
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=171
PRIMER_PAIR_1_PRODUCT_TM=70.8
PRIMER_PAIR_2_PENALTY=1.005626
PRIMER_LEFT_2_PENALTY=0.231272
PRIMER_RIGHT_2_PENALTY=0.774354
PRIMER_LEFT_2_SEQUENCE=ACCTTCAAGGCGTGTGTTCT
PRIMER_RIGHT_2_SEQUENCE=TACACACATTCGTACACGCG
PRIMER_LEFT_2=35,20
PRIMER_RIGHT_2=204,20
PRIMER_LEFT_2_TM=59.769
PRIMER_RIGHT_2_TM=59.226
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=11.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=170
PRIMER_PAIR_2_PRODUCT_TM=70.8
PRIMER_PAIR_3_PENALTY=1.005626
PRIMER_LEFT_3_PENALTY=0.231272
PRIMER_RIGHT_3_PENALTY=0.774354
PRIMER_LEFT_3_SEQUENCE=AGTCAACCTTCAAGGCGTGT
PRIMER_RIGHT_3_SEQUENCE=TACACACATTCGTACACGCG
PRIMER_LEFT_3=30,20
PRIMER_RIGHT_3=204,20
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=59.226
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=11.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=175
PRIMER_PAIR_3_PRODUCT_TM=71.0
PRIMER_PAIR_4_PENALTY=1.064943
PRIMER_LEFT_4_PENALTY=0.290589
PRIMER_RIGHT_4_PENALTY=0.774354
PRIMER_LEFT_4_SEQUENCE=CCTTCAAGGCGTGTGTTCTT
PRIMER_RIGHT_4_SEQUENCE=TACACACATTCGTACACGCG
PRIMER_LEFT_4=36,20
PRIMER_RIGHT_4=204,20
PRIMER_LEFT_4_TM=60.291
PRIMER_RIGHT_4_TM=59.226
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=11.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=169
PRIMER_PAIR_4_PRODUCT_TM=70.9
=
SEQUENCE_ID=15.RHAP34FF3.seq
SEQUENCE_TEMPLATE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=8,193
SEQUENCE_TARGET=119,24
SEQUENCE_TARGET=154,25
P3_COMMENT=CL is TGAGAACAGTCAAGGGGACA
P3_COMMENT=CR is CCACTTAAGAGCCCAGCAAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.148110
PRIMER_LEFT_0_PENALTY=0.770184
PRIMER_RIGHT_0_PENALTY=0.377925
PRIMER_LEFT_0_SEQUENCE=TGAGAACAGTCAAGGGGACA
PRIMER_RIGHT_0_SEQUENCE=CCACTTAAGAGCCCAGCAAA
PRIMER_LEFT_0=31,20
PRIMER_RIGHT_0=200,20
PRIMER_LEFT_0_TM=59.230
PRIMER_RIGHT_0_TM=60.378
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=170
PRIMER_PAIR_0_PRODUCT_TM=69.2
PRIMER_PAIR_1_PENALTY=1.660265
PRIMER_LEFT_1_PENALTY=1.282340
PRIMER_RIGHT_1_PENALTY=0.377925
PRIMER_LEFT_1_SEQUENCE=TGAGAACAGTCAAGGGGACAG
PRIMER_RIGHT_1_SEQUENCE=CCACTTAAGAGCCCAGCAAA
PRIMER_LEFT_1=31,21
PRIMER_RIGHT_1=200,20
PRIMER_LEFT_1_TM=60.282
PRIMER_RIGHT_1_TM=60.378
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=170
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=1.660265
PRIMER_LEFT_2_PENALTY=1.282340
PRIMER_RIGHT_2_PENALTY=0.377925
PRIMER_LEFT_2_SEQUENCE=CTGAGAACAGTCAAGGGGACA
PRIMER_RIGHT_2_SEQUENCE=CCACTTAAGAGCCCAGCAAA
PRIMER_LEFT_2=30,21
PRIMER_RIGHT_2=200,20
PRIMER_LEFT_2_TM=60.282
PRIMER_RIGHT_2_TM=60.378
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=69.3
PRIMER_PAIR_3_PENALTY=2.596154
PRIMER_LEFT_3_PENALTY=0.770184
PRIMER_RIGHT_3_PENALTY=1.825970
PRIMER_LEFT_3_SEQUENCE=TGAGAACAGTCAAGGGGACA
PRIMER_RIGHT_3_SEQUENCE=CACTTAAGAGCCCAGCAAAA
PRIMER_LEFT_3=31,20
PRIMER_RIGHT_3=199,20
PRIMER_LEFT_3_TM=59.230
PRIMER_RIGHT_3_TM=58.174
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=169
PRIMER_PAIR_3_PRODUCT_TM=69.0
PRIMER_PAIR_4_PENALTY=2.606323
PRIMER_LEFT_4_PENALTY=0.770184
PRIMER_RIGHT_4_PENALTY=1.836139
PRIMER_LEFT_4_SEQUENCE=TGAGAACAGTCAAGGGGACA
PRIMER_RIGHT_4_SEQUENCE=CACTTAAGAGCCCAGCAAAAG
PRIMER_LEFT_4=31,20
PRIMER_RIGHT_4=199,21
PRIMER_LEFT_4_TM=59.230
PRIMER_RIGHT_4_TM=59.164
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=169
PRIMER_PAIR_4_PRODUCT_TM=69.0
=
SEQUENCE_ID=16.RHAP33FF4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTTTGGACTCTGATGGAAGAACAGAATGAAAAGGATTATGGCCTCTAAGGGCAAAGGTACTCCACAGGCTTTGTCTTCATGTGGGTGTCGAACAACTAGAATTGGGCTTTCCCAAAACATGTTGTCTGTAGATGGGATATCTGTGTCTTTAAAATTCATTTATTTATTTACTCACTTTATATCCCAAAACTGGTGCTCCATCTTCATGGGATACCAACACCCATTGTTAGGGGAACCCTTAAAAAAACAAAGCGTGCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGCAATGGGGGGTGAGGAGNCAGGAGGAGNNNNCNNAGGACAGAGGACCGAGT
SEQUENCE_INCLUDED_REGION=17,363
SEQUENCE_TARGET=278,41
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=16.RHAP34FF4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAGAAAACTCCGGAAGAGACCACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACCCATTCACGTGGGAGAGGGAGTGGACGGGCTCATTCTTGGATCTGCGGAGGGGTGGTTCGTAGGCACCTGGGAAGGGACTCTCCCATTTCTTACTGGTGTGGTGGGGTCTTGAAAGGAAGCTGCCCAAGCTGGGCGCTGGGCGGATGGCTCACGCCTTTAATTCCCCCGCACTGGGGGGGTAGCCAGTAGGAGAGATGGACCACCGANTTC
SEQUENCE_INCLUDED_REGION=20,279
SEQUENCE_TARGET=46,38
P3_COMMENT=CL is AACTCCGGAAGAGACCACCT
P3_COMMENT=CR is GAAATGGGAGAGTCCCTTCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.236990
PRIMER_LEFT_0_PENALTY=0.110797
PRIMER_RIGHT_0_PENALTY=0.126193
PRIMER_LEFT_0_SEQUENCE=AACTCCGGAAGAGACCACCT
PRIMER_RIGHT_0_SEQUENCE=GAAATGGGAGAGTCCCTTCC
PRIMER_LEFT_0=25,20
PRIMER_RIGHT_0=177,20
PRIMER_LEFT_0_TM=60.111
PRIMER_RIGHT_0_TM=59.874
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=153
PRIMER_PAIR_0_PRODUCT_TM=73.7
PRIMER_PAIR_1_PENALTY=0.385991
PRIMER_LEFT_1_PENALTY=0.110797
PRIMER_RIGHT_1_PENALTY=0.275194
PRIMER_LEFT_1_SEQUENCE=AACTCCGGAAGAGACCACCT
PRIMER_RIGHT_1_SEQUENCE=ACCCCACCACACCAGTAAGA
PRIMER_LEFT_1=25,20
PRIMER_RIGHT_1=195,20
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=60.275
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=171
PRIMER_PAIR_1_PRODUCT_TM=74.6
PRIMER_PAIR_2_PENALTY=0.385991
PRIMER_LEFT_2_PENALTY=0.110797
PRIMER_RIGHT_2_PENALTY=0.275194
PRIMER_LEFT_2_SEQUENCE=AACTCCGGAAGAGACCACCT
PRIMER_RIGHT_2_SEQUENCE=GACCCCACCACACCAGTAAG
PRIMER_LEFT_2=25,20
PRIMER_RIGHT_2=196,20
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=60.275
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=172
PRIMER_PAIR_2_PRODUCT_TM=74.8
PRIMER_PAIR_3_PENALTY=0.385991
PRIMER_LEFT_3_PENALTY=0.110797
PRIMER_RIGHT_3_PENALTY=0.275194
PRIMER_LEFT_3_SEQUENCE=AACTCCGGAAGAGACCACCT
PRIMER_RIGHT_3_SEQUENCE=AGACCCCACCACACCAGTAA
PRIMER_LEFT_3=25,20
PRIMER_RIGHT_3=197,20
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=60.275
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=173
PRIMER_PAIR_3_PRODUCT_TM=74.7
PRIMER_PAIR_4_PENALTY=0.385991
PRIMER_LEFT_4_PENALTY=0.110797
PRIMER_RIGHT_4_PENALTY=0.275194
PRIMER_LEFT_4_SEQUENCE=AACTCCGGAAGAGACCACCT
PRIMER_RIGHT_4_SEQUENCE=AAGACCCCACCACACCAGTA
PRIMER_LEFT_4=25,20
PRIMER_RIGHT_4=198,20
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=74.6
=
SEQUENCE_ID=17.RHAP34FF5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCTTGACTTCTATGTATGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATCTTATACATCTCTATTGTGTCCTTGTCATGTGGAGGTCAGAGATGAATATACATCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTTGTGGTGNACATCTCTCTCTGTTTATATTGTGGCTTGTCATGGGGAGGCCAAGAGGTTTAAGGACTCACACAGCTCT
SEQUENCE_INCLUDED_REGION=18,285
SEQUENCE_TARGET=40,50
SEQUENCE_TARGET=154,26
SEQUENCE_TARGET=188,33
P3_COMMENT=CL is TCCTTGTCATGTGGAGGTCA
P3_COMMENT=CR is CTTAAACCTCTTGGCCTCCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.155493
PRIMER_LEFT_0_PENALTY=0.087977
PRIMER_RIGHT_0_PENALTY=0.067516
PRIMER_LEFT_0_SEQUENCE=TCCTTGTCATGTGGAGGTCA
PRIMER_RIGHT_0_SEQUENCE=CTTAAACCTCTTGGCCTCCC
PRIMER_LEFT_0=114,20
PRIMER_RIGHT_0=287,20
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=60.068
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=68.2
PRIMER_PAIR_1_PENALTY=0.155493
PRIMER_LEFT_1_PENALTY=0.087977
PRIMER_RIGHT_1_PENALTY=0.067516
PRIMER_LEFT_1_SEQUENCE=TCCTTGTCATGTGGAGGTCA
PRIMER_RIGHT_1_SEQUENCE=CCTTAAACCTCTTGGCCTCC
PRIMER_LEFT_1=114,20
PRIMER_RIGHT_1=288,20
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.068
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=68.4
PRIMER_PAIR_2_PENALTY=0.970243
PRIMER_LEFT_2_PENALTY=0.902727
PRIMER_RIGHT_2_PENALTY=0.067516
PRIMER_LEFT_2_SEQUENCE=CCTTGTCATGTGGAGGTCAG
PRIMER_RIGHT_2_SEQUENCE=CTTAAACCTCTTGGCCTCCC
PRIMER_LEFT_2=115,20
PRIMER_RIGHT_2=287,20
PRIMER_LEFT_2_TM=59.097
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=173
PRIMER_PAIR_2_PRODUCT_TM=68.3
PRIMER_PAIR_3_PENALTY=0.970243
PRIMER_LEFT_3_PENALTY=0.902727
PRIMER_RIGHT_3_PENALTY=0.067516
PRIMER_LEFT_3_SEQUENCE=CCTTGTCATGTGGAGGTCAG
PRIMER_RIGHT_3_SEQUENCE=CCTTAAACCTCTTGGCCTCC
PRIMER_LEFT_3=115,20
PRIMER_RIGHT_3=288,20
PRIMER_LEFT_3_TM=59.097
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=68.5
PRIMER_PAIR_4_PENALTY=1.781024
PRIMER_LEFT_4_PENALTY=0.087977
PRIMER_RIGHT_4_PENALTY=1.693048
PRIMER_LEFT_4_SEQUENCE=TCCTTGTCATGTGGAGGTCA
PRIMER_RIGHT_4_SEQUENCE=CCCCATGACAAGCCACAATA
PRIMER_LEFT_4=114,20
PRIMER_RIGHT_4=270,20
PRIMER_LEFT_4_TM=60.088
PRIMER_RIGHT_4_TM=61.693
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=10.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=157
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=18.RHAP34FB6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACATGGTTATATGTCAAAATAATTTAATTTGTTGGTTGTTTCTTCCCAAAAATTACTTTAGTAAGAGTCCAAAAACTATTATTAATTAATATTAAGTTTTNGGNGNTGGTGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGATGTGTGCGTGATGTGAGCATGTTCCATGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=13,197
SEQUENCE_TARGET=127,46
P3_COMMENT=CL is TGTTGGTTGTTTCTTCCCAA
P3_COMMENT=CR is CCATGGAACATGCTCACATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.079588
PRIMER_LEFT_0_PENALTY=1.004672
PRIMER_RIGHT_0_PENALTY=0.074917
PRIMER_LEFT_0_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
PRIMER_RIGHT_0_SEQUENCE=CCATGGAACATGCTCACATC
PRIMER_LEFT_0=42,20
PRIMER_RIGHT_0=208,20
PRIMER_LEFT_0_TM=58.995
PRIMER_RIGHT_0_TM=59.925
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=167
PRIMER_PAIR_0_PRODUCT_TM=66.6
PRIMER_PAIR_1_PENALTY=1.079588
PRIMER_LEFT_1_PENALTY=1.004672
PRIMER_RIGHT_1_PENALTY=0.074917
PRIMER_LEFT_1_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
PRIMER_RIGHT_1_SEQUENCE=CCATGGAACATGCTCACATC
PRIMER_LEFT_1=41,20
PRIMER_RIGHT_1=208,20
PRIMER_LEFT_1_TM=58.995
PRIMER_RIGHT_1_TM=59.925
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=168
PRIMER_PAIR_1_PRODUCT_TM=66.6
PRIMER_PAIR_2_PENALTY=1.180452
PRIMER_LEFT_2_PENALTY=1.004672
PRIMER_RIGHT_2_PENALTY=0.175780
PRIMER_LEFT_2_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
PRIMER_RIGHT_2_SEQUENCE=TCACATCACGCACACATCAC
PRIMER_LEFT_2=42,20
PRIMER_RIGHT_2=195,20
PRIMER_LEFT_2_TM=58.995
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=154
PRIMER_PAIR_2_PRODUCT_TM=65.3
PRIMER_PAIR_3_PENALTY=1.180452
PRIMER_LEFT_3_PENALTY=1.004672
PRIMER_RIGHT_3_PENALTY=0.175780
PRIMER_LEFT_3_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
PRIMER_RIGHT_3_SEQUENCE=TCACATCACGCACACATCAC
PRIMER_LEFT_3=41,20
PRIMER_RIGHT_3=195,20
PRIMER_LEFT_3_TM=58.995
PRIMER_RIGHT_3_TM=60.176
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=155
PRIMER_PAIR_3_PRODUCT_TM=65.3
PRIMER_PAIR_4_PENALTY=1.336562
PRIMER_LEFT_4_PENALTY=1.004672
PRIMER_RIGHT_4_PENALTY=0.331890
PRIMER_LEFT_4_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
PRIMER_RIGHT_4_SEQUENCE=CTCACATCACGCACACATCA
PRIMER_LEFT_4=42,20
PRIMER_RIGHT_4=196,20
PRIMER_LEFT_4_TM=58.995
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=155
PRIMER_PAIR_4_PRODUCT_TM=65.6
=
SEQUENCE_ID=18.RHAP34FF6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNCCGTCTCAGTTAAAAGCAAATCTTGCCTCCATTCTGCGGGTTATTTTTGATGGTTTCCTCCTCATCTTCACTGGTCTGTTCTGGTGTTTGCTCCTAGGATTTGTCACGTGAATCCTGACTGATCCGACTTGCTTGTTTTTTTTTGCTCTTTCTTTAGTTCTCTATTTCTTTGCTCTTTTTCCTTGTACGGAAAGTTTTTTGCTTTCTCCTTTTTTCTTAAATGCACGCATGTGACTATTGTGATGGANNNNNNNNNNNNNNNNNNNNNNNNNATNNNN
SEQUENCE_INCLUDED_REGION=9,274
SEQUENCE_TARGET=256,25
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=19.RHAP34FB7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTTTTCCACTTGTCTTGTTTCCAAACCTCCCTTTCCATCGAAAGCCATATAGTCATCTTTGTCCTATTATCAGCGNNNNNNNNNNNNNNNNNNNNANANANANGNGNGNGNGNGNANNNCNATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGGGNANAGAGNAATATGTGNATATGNGNANATANGGGGAAANACACANANANGGGGGNAGNGNGCGCGNAAGNGNNGNNAGAANCANGGGANNAGNAGAGTGNNNNGNGGGGGNAG
SEQUENCE_INCLUDED_REGION=19,286
SEQUENCE_TARGET=93,20
SEQUENCE_TARGET=147,38
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=20.RHAP33FB8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCTAGTAATCCTATTAGTTACACATGTAAATAACTAAGTGTAGCCCCTTAGGTGGAGACACCAGAGGGACAAGTGCATATTAGTTATTAGTAAACAAAAGAGCAAAAGGCTCTGAAGCTCTAGATCAACAAACAAAATCTATGGATGTAAAGAAATAATAAAGTCCAGGACAGAAGGAAATGAAACGTTGACAAAGTAATACCAATAATCAGTGAAACTAAAAGATGAGTGAGATTCACAGTTACTATTTGTAAGAGGTACATAGAATATTCGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGTGCCTTAAGTACATATATATTTTATAGAAATGNATTACTTTAGG
SEQUENCE_INCLUDED_REGION=23,356
SEQUENCE_TARGET=299,30
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=20.RHAP34FB8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACAAAGTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACTGAGCTTCTAGAAGTAATATAGTTGCCAGAGANNANGGGGAAATATTGAGAACTTGCTAAATGTGTGCAGTCATAGTCAGACAAACAAAATAAGCTCTGGAGATCCATTACACAGATCGTGATTACAGCTCCCAATATTATTTCGAATAAGACACAGTAACTATACAAGGAAGCATTTGGATGTTCTTATACAGAAGAAATGATA
SEQUENCE_INCLUDED_REGION=19,278
SEQUENCE_TARGET=30,56
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=20.RHAP34FF8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAAATGCCAGCATGCAAAACTATAAAAGGATAAAAATGAAAATAGAAATGAAGTGTTGGCAGGAGCAGAAAGTGATGGCGCAGAATAGGAGGGCATTTCACAATAGTCCATGTGGCAAGAACTGTAGCCACTGATACATTTCTTTTTTAGTTCCTCTTTACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTTTAAAAATCTGCTTAAAAGAGCAGACAATATTAAGTTCCTG
SEQUENCE_INCLUDED_REGION=20,279
SEQUENCE_TARGET=184,70
P3_COMMENT=CL is TGTGGCAAGAACTGTAGCCA
P3_COMMENT=CR is TGTCTGCTCTTTTAAGCAGATTTTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.475313
PRIMER_LEFT_0_PENALTY=0.449441
PRIMER_RIGHT_0_PENALTY=5.025871
PRIMER_LEFT_0_SEQUENCE=TGTGGCAAGAACTGTAGCCA
PRIMER_RIGHT_0_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTTT
PRIMER_LEFT_0=130,20
PRIMER_RIGHT_0=284,25
PRIMER_LEFT_0_TM=60.449
PRIMER_RIGHT_0_TM=59.974
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=32.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=155
PRIMER_PAIR_0_PRODUCT_TM=63.7
PRIMER_PAIR_1_PENALTY=5.475313
PRIMER_LEFT_1_PENALTY=0.449441
PRIMER_RIGHT_1_PENALTY=5.025871
PRIMER_LEFT_1_SEQUENCE=TGTGGCAAGAACTGTAGCCA
PRIMER_RIGHT_1_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTTT
PRIMER_LEFT_1=130,20
PRIMER_RIGHT_1=285,25
PRIMER_LEFT_1_TM=60.449
PRIMER_RIGHT_1_TM=59.974
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=32.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=156
PRIMER_PAIR_1_PRODUCT_TM=63.7
PRIMER_PAIR_2_PENALTY=5.626457
PRIMER_LEFT_2_PENALTY=0.449441
PRIMER_RIGHT_2_PENALTY=5.177016
PRIMER_LEFT_2_SEQUENCE=TGTGGCAAGAACTGTAGCCA
PRIMER_RIGHT_2_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTT
PRIMER_LEFT_2=130,20
PRIMER_RIGHT_2=284,24
PRIMER_LEFT_2_TM=60.449
PRIMER_RIGHT_2_TM=58.823
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=155
PRIMER_PAIR_2_PRODUCT_TM=63.7
PRIMER_PAIR_3_PENALTY=5.626457
PRIMER_LEFT_3_PENALTY=0.449441
PRIMER_RIGHT_3_PENALTY=5.177016
PRIMER_LEFT_3_SEQUENCE=TGTGGCAAGAACTGTAGCCA
PRIMER_RIGHT_3_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTT
PRIMER_LEFT_3=130,20
PRIMER_RIGHT_3=285,24
PRIMER_LEFT_3_TM=60.449
PRIMER_RIGHT_3_TM=58.823
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=156
PRIMER_PAIR_3_PRODUCT_TM=63.7
PRIMER_PAIR_4_PENALTY=5.891375
PRIMER_LEFT_4_PENALTY=0.449441
PRIMER_RIGHT_4_PENALTY=5.441933
PRIMER_LEFT_4_SEQUENCE=TGTGGCAAGAACTGTAGCCA
PRIMER_RIGHT_4_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTT
PRIMER_LEFT_4=130,20
PRIMER_RIGHT_4=284,23
PRIMER_LEFT_4_TM=60.449
PRIMER_RIGHT_4_TM=57.558
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=155
PRIMER_PAIR_4_PRODUCT_TM=63.7
=
SEQUENCE_ID=21.RHAP33FB9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTCTTCATCCAGTCACCAACACGGGGCAGACTCAGGCTCATTGAGAAGCCTGCAGGTACAGAATGGCCCTGTTCCCTCTTACTAAATTTTGCTTGCAGTCAAGTTGCATAGCATGGAGTCCTGGTTGCAGGAATATCTCTATAGAGTTCTCAGATGCCCCCTCTCAGTGTTTCCATAAAACCTGGCTACCACGTGATGGAAGAAACAGCCTGATGTTACCATTTCCTTATTTTCACATGTATCTGCATGCATANNNNNNNNNNNNNNNNNNNNNNNNCTCTTGCTTGTAGAAGCTCACCAAAGCCATGCTTATACTACAGCCTGGTCTATTTCACATCCTTCAAGAGGTATACACAC
SEQUENCE_INCLUDED_REGION=21,357
SEQUENCE_TARGET=274,24
P3_COMMENT=CL is AGTTCTCAGATGCCCCCTCT
P3_COMMENT=CR is CATGGCTTTGGTGAGCTTCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.613091
PRIMER_LEFT_0_PENALTY=0.216148
PRIMER_RIGHT_0_PENALTY=0.396944
PRIMER_LEFT_0_SEQUENCE=AGTTCTCAGATGCCCCCTCT
PRIMER_RIGHT_0_SEQUENCE=CATGGCTTTGGTGAGCTTCT
PRIMER_LEFT_0=165,20
PRIMER_RIGHT_0=328,20
PRIMER_LEFT_0_TM=60.216
PRIMER_RIGHT_0_TM=60.397
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=164
PRIMER_PAIR_0_PRODUCT_TM=71.7
PRIMER_PAIR_1_PENALTY=0.613091
PRIMER_LEFT_1_PENALTY=0.216148
PRIMER_RIGHT_1_PENALTY=0.396944
PRIMER_LEFT_1_SEQUENCE=AGAGTTCTCAGATGCCCCCT
PRIMER_RIGHT_1_SEQUENCE=CATGGCTTTGGTGAGCTTCT
PRIMER_LEFT_1=163,20
PRIMER_RIGHT_1=328,20
PRIMER_LEFT_1_TM=60.216
PRIMER_RIGHT_1_TM=60.397
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=166
PRIMER_PAIR_1_PRODUCT_TM=71.8
PRIMER_PAIR_2_PENALTY=0.919387
PRIMER_LEFT_2_PENALTY=0.522443
PRIMER_RIGHT_2_PENALTY=0.396944
PRIMER_LEFT_2_SEQUENCE=AAACCTGGCTACCACGTGAT
PRIMER_RIGHT_2_SEQUENCE=CATGGCTTTGGTGAGCTTCT
PRIMER_LEFT_2=198,20
PRIMER_RIGHT_2=328,20
PRIMER_LEFT_2_TM=59.478
PRIMER_RIGHT_2_TM=60.397
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=131
PRIMER_PAIR_2_PRODUCT_TM=69.7
PRIMER_PAIR_3_PENALTY=0.947035
PRIMER_LEFT_3_PENALTY=0.550092
PRIMER_RIGHT_3_PENALTY=0.396944
PRIMER_LEFT_3_SEQUENCE=AAAACCTGGCTACCACGTGA
PRIMER_RIGHT_3_SEQUENCE=CATGGCTTTGGTGAGCTTCT
PRIMER_LEFT_3=197,20
PRIMER_RIGHT_3=328,20
PRIMER_LEFT_3_TM=60.550
PRIMER_RIGHT_3_TM=60.397
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=132
PRIMER_PAIR_3_PRODUCT_TM=69.6
PRIMER_PAIR_4_PENALTY=1.158416
PRIMER_LEFT_4_PENALTY=0.761472
PRIMER_RIGHT_4_PENALTY=0.396944
PRIMER_LEFT_4_SEQUENCE=TAGAGTTCTCAGATGCCCCC
PRIMER_RIGHT_4_SEQUENCE=CATGGCTTTGGTGAGCTTCT
PRIMER_LEFT_4=162,20
PRIMER_RIGHT_4=328,20
PRIMER_LEFT_4_TM=59.239
PRIMER_RIGHT_4_TM=60.397
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=167
PRIMER_PAIR_4_PRODUCT_TM=71.8
=
SEQUENCE_ID=21.RHAP33FF9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTAGCAGTGAAACTAAGTTTATGGCTGGGAGTCATTAGAACATGAGGAACCGGTTAAAGGGTGACAGCGTTAGGACTGAGAACCTGTGCTTTAGAGCAAGCCTCAACTGCCCACTTGCAGACAGCAGTTCTCCAGTCATTTTCAACTTGTTCATTTCCAATGGAGACAAGATATCTATTTATCACGTGCAGCATGATAAATAANNNNNNNNNNNNNNNNNACATCCCCAACAGTGAGGACCAGGCTCTACTTGAAAACCCATTTCCACACCCACTCTCACTCATGAACAGACATACACATAATGAATGTATTTTTGTGAAGCACCCATTTCATACCTACCAAGAAAAATAATTCATTTTCT
SEQUENCE_INCLUDED_REGION=27,367
SEQUENCE_TARGET=236,17
P3_COMMENT=CL is CCTGTGCTTTAGAGCAAGCC
P3_COMMENT=CR is GTCCTCACTGTTGGGGATGT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.337261
PRIMER_LEFT_0_PENALTY=0.154023
PRIMER_RIGHT_0_PENALTY=0.183238
PRIMER_LEFT_0_SEQUENCE=CCTGTGCTTTAGAGCAAGCC
PRIMER_RIGHT_0_SEQUENCE=GTCCTCACTGTTGGGGATGT
PRIMER_LEFT_0=115,20
PRIMER_RIGHT_0=272,20
PRIMER_LEFT_0_TM=60.154
PRIMER_RIGHT_0_TM=59.817
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=158
PRIMER_PAIR_0_PRODUCT_TM=71.9
PRIMER_PAIR_1_PENALTY=0.516560
PRIMER_LEFT_1_PENALTY=0.333322
PRIMER_RIGHT_1_PENALTY=0.183238
PRIMER_LEFT_1_SEQUENCE=TGCAGACAGCAGTTCTCCAG
PRIMER_RIGHT_1_SEQUENCE=GTCCTCACTGTTGGGGATGT
PRIMER_LEFT_1=148,20
PRIMER_RIGHT_1=272,20
PRIMER_LEFT_1_TM=60.333
PRIMER_RIGHT_1_TM=59.817
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=0.561053
PRIMER_LEFT_2_PENALTY=0.377815
PRIMER_RIGHT_2_PENALTY=0.183238
PRIMER_LEFT_2_SEQUENCE=CCACTTGCAGACAGCAGTTC
PRIMER_RIGHT_2_SEQUENCE=GTCCTCACTGTTGGGGATGT
PRIMER_LEFT_2=143,20
PRIMER_RIGHT_2=272,20
PRIMER_LEFT_2_TM=59.622
PRIMER_RIGHT_2_TM=59.817
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=130
PRIMER_PAIR_2_PRODUCT_TM=69.8
PRIMER_PAIR_3_PENALTY=0.626637
PRIMER_LEFT_3_PENALTY=0.333322
PRIMER_RIGHT_3_PENALTY=0.293316
PRIMER_LEFT_3_SEQUENCE=TGCAGACAGCAGTTCTCCAG
PRIMER_RIGHT_3_SEQUENCE=TGAGTGAGAGTGGGTGTGGA
PRIMER_LEFT_3=148,20
PRIMER_RIGHT_3=315,20
PRIMER_LEFT_3_TM=60.333
PRIMER_RIGHT_3_TM=60.293
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=168
PRIMER_PAIR_3_PRODUCT_TM=72.0
PRIMER_PAIR_4_PENALTY=0.671131
PRIMER_LEFT_4_PENALTY=0.377815
PRIMER_RIGHT_4_PENALTY=0.293316
PRIMER_LEFT_4_SEQUENCE=CCACTTGCAGACAGCAGTTC
PRIMER_RIGHT_4_SEQUENCE=TGAGTGAGAGTGGGTGTGGA
PRIMER_LEFT_4=143,20
PRIMER_RIGHT_4=315,20
PRIMER_LEFT_4_TM=59.622
PRIMER_RIGHT_4_TM=60.293
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=173
PRIMER_PAIR_4_PRODUCT_TM=72.3
=
SEQUENCE_ID=21.RHAP34FB9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGTAAAACATTCCTTCTTTCTAGCAATCATTCGTATACAAACTAAAACATAATCTGTGTCTTGCTTACAAGCATTTCAAATATTCAAAAATGTTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAGTGTATGTCTATGTTTATATGTGTCCTTGTTTATATGTGTGTTCATGTTTATAGGTATGTGTCTATGTTTATATGTGTGTCCATGTTTATATGTGTGCCTGTTCATATGTGTGTTCATGNTTATAGGTGTGTGTCCATGTTTATAT
SEQUENCE_INCLUDED_REGION=20,274
SEQUENCE_TARGET=117,26
P3_COMMENT=CL is TGTCTTGCTTACAAGCATTTCAA
P3_COMMENT=CR is CAGGCACACATATAAACATGGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.622123
PRIMER_LEFT_0_PENALTY=3.061184
PRIMER_RIGHT_0_PENALTY=3.560938
PRIMER_LEFT_0_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
PRIMER_RIGHT_0_SEQUENCE=CAGGCACACATATAAACATGGA
PRIMER_LEFT_0=75,23
PRIMER_RIGHT_0=248,22
PRIMER_LEFT_0_TM=59.939
PRIMER_RIGHT_0_TM=58.439
PRIMER_LEFT_0_GC_PERCENT=34.783
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=67.1
PRIMER_PAIR_1_PENALTY=6.767082
PRIMER_LEFT_1_PENALTY=3.061184
PRIMER_RIGHT_1_PENALTY=3.705897
PRIMER_LEFT_1_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
PRIMER_RIGHT_1_SEQUENCE=GGCACACATATAAACATGGACA
PRIMER_LEFT_1=75,23
PRIMER_RIGHT_1=246,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=58.294
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=172
PRIMER_PAIR_1_PRODUCT_TM=66.9
PRIMER_PAIR_2_PENALTY=6.776871
PRIMER_LEFT_2_PENALTY=3.061184
PRIMER_RIGHT_2_PENALTY=3.715687
PRIMER_LEFT_2_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
PRIMER_RIGHT_2_SEQUENCE=AGGCACACATATAAACATGGACA
PRIMER_LEFT_2=75,23
PRIMER_RIGHT_2=247,23
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=59.284
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=173
PRIMER_PAIR_2_PRODUCT_TM=66.9
PRIMER_PAIR_3_PENALTY=6.776871
PRIMER_LEFT_3_PENALTY=3.061184
PRIMER_RIGHT_3_PENALTY=3.715687
PRIMER_LEFT_3_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
PRIMER_RIGHT_3_SEQUENCE=CAGGCACACATATAAACATGGAC
PRIMER_LEFT_3=75,23
PRIMER_RIGHT_3=248,23
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=59.284
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=67.1
PRIMER_PAIR_4_PENALTY=6.776871
PRIMER_LEFT_4_PENALTY=3.061184
PRIMER_RIGHT_4_PENALTY=3.715687
PRIMER_LEFT_4_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
PRIMER_RIGHT_4_SEQUENCE=ACAGGCACACATATAAACATGGA
PRIMER_LEFT_4=75,23
PRIMER_RIGHT_4=249,23
PRIMER_LEFT_4_TM=59.939
PRIMER_RIGHT_4_TM=59.284
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=175
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=22.RHAP33FF10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCTGCCTCCCAGGCAGGATGGCCGCTCACGTTGAGGGATGGCTCCCTACAGAGGCAGCAGGGGTAATGGGGAGCTTATCTTACTTCATTCACTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGANNNNNNNNNNNNNNNNCTTATAATTTTGGGAGTTTTTTTCTTTTCATTATCTTTGGCCTCCAAAGATTTTAAAGAGTCNAGCCCAACTTCTTGCAGGTCTTCAGGGAACGCCCCAGTAAATCAAGCTGCAGGCAATGGACANGGAGAATACACCTTCCTCAAGCNCCCGAAAGAAGAGCCAAAAGGNAGAAGCCCACTGANNCCCGC
SEQUENCE_INCLUDED_REGION=18,370
SEQUENCE_TARGET=113,63
SEQUENCE_TARGET=181,16
P3_COMMENT=CL is GGGGTAATGGGGAGCTTATC
P3_COMMENT=CR is TTGGAGGCCAAAGATAATGAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.839308
PRIMER_LEFT_0_PENALTY=0.372603
PRIMER_RIGHT_0_PENALTY=1.466705
PRIMER_LEFT_0_SEQUENCE=GGGGTAATGGGGAGCTTATC
PRIMER_RIGHT_0_SEQUENCE=TGGAGGCCAAAGATAATGAAA
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=242,21
PRIMER_LEFT_0_TM=59.627
PRIMER_RIGHT_0_TM=59.533
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=167
PRIMER_PAIR_0_PRODUCT_TM=64.4
PRIMER_PAIR_1_PENALTY=1.839308
PRIMER_LEFT_1_PENALTY=0.372603
PRIMER_RIGHT_1_PENALTY=1.466705
PRIMER_LEFT_1_SEQUENCE=GGGGTAATGGGGAGCTTATC
PRIMER_RIGHT_1_SEQUENCE=TTGGAGGCCAAAGATAATGAA
PRIMER_LEFT_1=76,20
PRIMER_RIGHT_1=243,21
PRIMER_LEFT_1_TM=59.627
PRIMER_RIGHT_1_TM=59.533
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=168
PRIMER_PAIR_1_PRODUCT_TM=64.4
PRIMER_PAIR_2_PENALTY=1.839308
PRIMER_LEFT_2_PENALTY=0.372603
PRIMER_RIGHT_2_PENALTY=1.466705
PRIMER_LEFT_2_SEQUENCE=GGGGTAATGGGGAGCTTATC
PRIMER_RIGHT_2_SEQUENCE=TTTGGAGGCCAAAGATAATGA
PRIMER_LEFT_2=76,20
PRIMER_RIGHT_2=244,21
PRIMER_LEFT_2_TM=59.627
PRIMER_RIGHT_2_TM=59.533
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=169
PRIMER_PAIR_2_PRODUCT_TM=64.4
PRIMER_PAIR_3_PENALTY=2.212475
PRIMER_LEFT_3_PENALTY=0.372603
PRIMER_RIGHT_3_PENALTY=1.839872
PRIMER_LEFT_3_SEQUENCE=GGGGTAATGGGGAGCTTATC
PRIMER_RIGHT_3_SEQUENCE=TGGAGGCCAAAGATAATGAA
PRIMER_LEFT_3=76,20
PRIMER_RIGHT_3=242,20
PRIMER_LEFT_3_TM=59.627
PRIMER_RIGHT_3_TM=58.160
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=64.4
PRIMER_PAIR_4_PENALTY=2.212475
PRIMER_LEFT_4_PENALTY=0.372603
PRIMER_RIGHT_4_PENALTY=1.839872
PRIMER_LEFT_4_SEQUENCE=GGGGTAATGGGGAGCTTATC
PRIMER_RIGHT_4_SEQUENCE=TTGGAGGCCAAAGATAATGA
PRIMER_LEFT_4=76,20
PRIMER_RIGHT_4=243,20
PRIMER_LEFT_4_TM=59.627
PRIMER_RIGHT_4_TM=58.160
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=168
PRIMER_PAIR_4_PRODUCT_TM=64.4
=
SEQUENCE_ID=22.RHAP34FB10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACACNCNCCCATGGTGAATGTGTTTCGNGCTCTTTTTCNTGGCTAATCAAGNGTTACCCCNTGGGGACATGAATCATGGAGAAACACAATACNAGAGAAANNTCCCAAGTTACCANNAGACCCAGTCCANGAATAGANGAGTAANNNNNNNNNNNNNNNNNAAAATATTGAAAANCCACTCACATACACAGNCATCNTACNTAACAAGNCACATACACATNNCACNGAGACACCACATGACACATACNCATAAATNAACAGACATAGAGAGAGACATAAAGACATGGACNCAAAGAGAANTATCATACACACAANGAAAGTAAAAAAATTACCACATTCAAACNCACNCANNGAGGACAANCAATTGNCAATTAAGAAAAGNCATACAAAAAATNCAAATNGAGANNGAT
SEQUENCE_INCLUDED_REGION=23,438
SEQUENCE_TARGET=23,27
SEQUENCE_TARGET=195,17
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=22.RHAP34FF10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNCCTCATATCCGGCATACATGCCTTCCCTGCCATCCTCAGCTGTATCCCCACAAACCTGAACTAAAATAAACCCTTCCCTGTTGCTTTTCTTAGACATTCTGTCACAGCAGTGAGAAAGGTAACTAAAGCAGAATCCTGGTTCCAGAAGTGGGTTGTCCTTGTGATAAACCCATCTCATGGCGCCTAGGCTTTTGCAATTGGTTTATGTGAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGATGGTGGGATGNT
SEQUENCE_INCLUDED_REGION=9,284
SEQUENCE_TARGET=223,50
P3_COMMENT=CL is GCAGAATCCTGGTTCCAGAA
P3_COMMENT=CR is CATCCCACCATCACAACC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.216911
PRIMER_LEFT_0_PENALTY=0.195358
PRIMER_RIGHT_0_PENALTY=4.021553
PRIMER_LEFT_0_SEQUENCE=GCAGAATCCTGGTTCCAGAA
PRIMER_RIGHT_0_SEQUENCE=CATCCCACCATCACAACC
PRIMER_LEFT_0=136,20
PRIMER_RIGHT_0=290,18
PRIMER_LEFT_0_TM=60.195
PRIMER_RIGHT_0_TM=57.978
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.556
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=155
PRIMER_PAIR_0_PRODUCT_TM=69.3
PRIMER_PAIR_1_PENALTY=4.218456
PRIMER_LEFT_1_PENALTY=0.196903
PRIMER_RIGHT_1_PENALTY=4.021553
PRIMER_LEFT_1_SEQUENCE=AGCAGAATCCTGGTTCCAGA
PRIMER_RIGHT_1_SEQUENCE=CATCCCACCATCACAACC
PRIMER_LEFT_1=135,20
PRIMER_RIGHT_1=290,18
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=57.978
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.556
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=156
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=4.351103
PRIMER_LEFT_2_PENALTY=0.329550
PRIMER_RIGHT_2_PENALTY=4.021553
PRIMER_LEFT_2_SEQUENCE=AAAGCAGAATCCTGGTTCCA
PRIMER_RIGHT_2_SEQUENCE=CATCCCACCATCACAACC
PRIMER_LEFT_2=133,20
PRIMER_RIGHT_2=290,18
PRIMER_LEFT_2_TM=59.670
PRIMER_RIGHT_2_TM=57.978
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.556
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=69.1
PRIMER_PAIR_3_PENALTY=4.420878
PRIMER_LEFT_3_PENALTY=0.399325
PRIMER_RIGHT_3_PENALTY=4.021553
PRIMER_LEFT_3_SEQUENCE=TGGTTCCAGAAGTGGGTTGT
PRIMER_RIGHT_3_SEQUENCE=CATCCCACCATCACAACC
PRIMER_LEFT_3=145,20
PRIMER_RIGHT_3=290,18
PRIMER_LEFT_3_TM=60.399
PRIMER_RIGHT_3_TM=57.978
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.556
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=146
PRIMER_PAIR_3_PRODUCT_TM=68.4
PRIMER_PAIR_4_PENALTY=4.472396
PRIMER_LEFT_4_PENALTY=0.450843
PRIMER_RIGHT_4_PENALTY=4.021553
PRIMER_LEFT_4_SEQUENCE=TCCAGAAGTGGGTTGTCCTT
PRIMER_RIGHT_4_SEQUENCE=CATCCCACCATCACAACC
PRIMER_LEFT_4=149,20
PRIMER_RIGHT_4=290,18
PRIMER_LEFT_4_TM=59.549
PRIMER_RIGHT_4_TM=57.978
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.556
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=142
PRIMER_PAIR_4_PRODUCT_TM=68.1
=
SEQUENCE_ID=23.RHAP34FF11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTACCAATTGTTCTTTATTTACAGGGAGGTCTACTCTGCCTCCTCATCCAGGCTTTCTTTCATTCATCTCCTTAGCTGATTGCATTAGATGCACACATTCATACACACAGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTGCTTCCCTGCAGAGGTCAAGTCTGGGAATAATACAAAGACTTCTCTGTAGGTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,240
SEQUENCE_TARGET=133,64
P3_COMMENT=CL is CCTCCTCATCCAGGCTTTCT
P3_COMMENT=CR is AGACTTGACCTCTGCAGGGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.739441
PRIMER_LEFT_0_PENALTY=0.725022
PRIMER_RIGHT_0_PENALTY=0.014419
PRIMER_LEFT_0_SEQUENCE=CCTCCTCATCCAGGCTTTCT
PRIMER_RIGHT_0_SEQUENCE=AGACTTGACCTCTGCAGGGA
PRIMER_LEFT_0=58,20
PRIMER_RIGHT_0=224,20
PRIMER_LEFT_0_TM=60.725
PRIMER_RIGHT_0_TM=59.986
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=167
PRIMER_PAIR_0_PRODUCT_TM=68.1
PRIMER_PAIR_1_PENALTY=1.110778
PRIMER_LEFT_1_PENALTY=0.725022
PRIMER_RIGHT_1_PENALTY=0.385756
PRIMER_LEFT_1_SEQUENCE=CCTCCTCATCCAGGCTTTCT
PRIMER_RIGHT_1_SEQUENCE=GACTTGACCTCTGCAGGGAA
PRIMER_LEFT_1=58,20
PRIMER_RIGHT_1=223,20
PRIMER_LEFT_1_TM=60.725
PRIMER_RIGHT_1_TM=60.386
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=166
PRIMER_PAIR_1_PRODUCT_TM=68.1
PRIMER_PAIR_2_PENALTY=1.110778
PRIMER_LEFT_2_PENALTY=0.725022
PRIMER_RIGHT_2_PENALTY=0.385756
PRIMER_LEFT_2_SEQUENCE=CCTCCTCATCCAGGCTTTCT
PRIMER_RIGHT_2_SEQUENCE=TTCCCAGACTTGACCTCTGC
PRIMER_LEFT_2=58,20
PRIMER_RIGHT_2=229,20
PRIMER_LEFT_2_TM=60.725
PRIMER_RIGHT_2_TM=60.386
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=172
PRIMER_PAIR_2_PRODUCT_TM=68.6
PRIMER_PAIR_3_PENALTY=1.153193
PRIMER_LEFT_3_PENALTY=1.138774
PRIMER_RIGHT_3_PENALTY=0.014419
PRIMER_LEFT_3_SEQUENCE=CCTCATCCAGGCTTTCTTTC
PRIMER_RIGHT_3_SEQUENCE=AGACTTGACCTCTGCAGGGA
PRIMER_LEFT_3=61,20
PRIMER_RIGHT_3=224,20
PRIMER_LEFT_3_TM=58.861
PRIMER_RIGHT_3_TM=59.986
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=164
PRIMER_PAIR_3_PRODUCT_TM=67.7
PRIMER_PAIR_4_PENALTY=1.153193
PRIMER_LEFT_4_PENALTY=1.138774
PRIMER_RIGHT_4_PENALTY=0.014419
PRIMER_LEFT_4_SEQUENCE=TCCTCATCCAGGCTTTCTTT
PRIMER_RIGHT_4_SEQUENCE=AGACTTGACCTCTGCAGGGA
PRIMER_LEFT_4=60,20
PRIMER_RIGHT_4=224,20
PRIMER_LEFT_4_TM=58.861
PRIMER_RIGHT_4_TM=59.986
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=165
PRIMER_PAIR_4_PRODUCT_TM=67.7
=
SEQUENCE_ID=24.RHAP33FB12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTTTTAGGTGTATAGGTTACATNAGCAAGGTTNCAGTTTAAACTTATTGGTTAGCATATTGACCTTTGAATCTGCTGGCTAGCAAGCATGGACAGATTATGACATGCCTTGGAACTCAATCTGGGACCAAAGGTTCAGGTGACTGTCAGCCAGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNTNTNNNNNNNNNNNNNNTCTCTCNGCGTCNCNNCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTNTGTGTGTNTGTTTTTTTNNGNNTATGTNNGGCAGCNNTATNGGNATTTGGGNNTANCGCTTTNNGACNNAANNGNGNCCNNNAGTGNAC
SEQUENCE_INCLUDED_REGION=23,356
SEQUENCE_TARGET=181,22
SEQUENCE_TARGET=209,14
SEQUENCE_TARGET=243,40
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=24.RHAP34FB12.seq
SEQUENCE_TEMPLATE=NNNGGGAGNTAAGCATGGAATCAAATCTGACAATAACTAACTAACAATAGTTGNCCTACCAAGTTGNAACCAAGCAACCATTTTGCTGAAAAANTGCTNTCTTTTTTCCANTTGANNTGGTTTTGAGACNTCCNNTANGTCAAAAAAAAATCNAGTGAANCCANTANGAGANGNTAGANGAGAGATATCANTANTCNTAGAGAGNTACTATACAAATATACATANTAACAAAAATAAGAGATACATAATCATAGNTACATAGATACATACATAGNTAACACATAAAAATAGAAAATAGAAACAATATATAGAAAGAATATATAAATNNATATATATAGAGACACNNNNNNNNNNNNNNAAACAAATATNCNTAATAGATAGNAAAGACNGNCAGACAGACCCANNNCNNAACNNNCTNTCTAACTACAAAC
SEQUENCE_INCLUDED_REGION=3,428
SEQUENCE_TARGET=344,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=26.RHAP33FC2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCGGCACATTTTTAAAATAGATTCAATCATTCAAATTCAATCACAAATTTTCTACCCCATAATAATTTCTAATCATTTCACAGTGCACACACTCCTGCATCTAAATGCCTTCTTCCACATGTCAAAATAGAATTCATGTCTATTACCACACCTCTGAACTATCAAATCATTCAAAATGAAGATCATAAAATGAAGATGCATGGAAATGATGTGTAATGCCATAATAGGATAGCTACAAGAGTAACAGCAGGAGTAGCCAGTCCTTTGGAGAGTCATATTTAACCATGTGCTAAATAAGAGTTAGCAATATAATGCTGTAAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAC
SEQUENCE_INCLUDED_REGION=23,361
SEQUENCE_TARGET=348,33
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=26.RHAP34FG2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTAGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCTGCTACTTAGAATCAGGAGCCACAGAACAGTGCAGTTCAGGAAACATCGGTTGACTTGATCATGAAGTCCTTAGAAGGGATGGAACCCACAGAACTGAAGATGTCCACAGAGAACNNTAGATAGACCATACTGAGGAACTCTGACATATCACTATCTACTGACATNTGATATATGAAGAGTATGACCACACAATCATATTATCATAGACNNTAATATATAGAAAAGNTTAGATNNCACAGAGATATATATCATGACTNTT
SEQUENCE_INCLUDED_REGION=29,334
SEQUENCE_TARGET=43,54
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=27.RHAP33FC3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNCTGCATTTTCTAGAAAGCATAACTAAAAGTACATGGGATCCTTGTGCGTGGCAGAGACATAATCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGAGAATANNNNNNNNNNNNNNNNNNNNAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANAGAGCANNNNNNNNNNNNNNNAGACCTGANTGANNNNNNNNNNNNNNNNNNNNNNCCAANGCTGGACANAGTGANATCAGTNCCCCNNACATNAC
SEQUENCE_INCLUDED_REGION=11,358
SEQUENCE_TARGET=77,58
SEQUENCE_TARGET=146,20
SEQUENCE_TARGET=171,38
SEQUENCE_TARGET=217,54
SEQUENCE_TARGET=281,14
SEQUENCE_TARGET=307,22
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=27.RHAP33FG3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCTAGGCAATGGATGGACTGGCACGGAGGTCTAATGCACAGAGCCACACTGCTAGAGAAGTCTGACCTAAAAAGTCAGTCAAATTAATAAATGAGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGAGGGGCTATGGGAATCTAAGAAACACAGTTTAAAATTGCNTTTAAATTAAATTTTTAGTTAGTATACCAAATAGCATTTTATTATGACAGTTTTGTNTACNNNNNNNNNNNNNNNNNNNNNNNNCTATGTATGTATATATAATTGAANTTGGCCTNTTATTTGNCCCCCCANNNCCCCCCNCAAGGNATANA
SEQUENCE_INCLUDED_REGION=16,365
SEQUENCE_TARGET=114,68
SEQUENCE_TARGET=289,24
P3_COMMENT=CL is GCACAGAGCCACACTGCTAG
P3_COMMENT=CR is TAGATTCCCATAGCCCCTCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.443596
PRIMER_LEFT_0_PENALTY=0.198986
PRIMER_RIGHT_0_PENALTY=0.244611
PRIMER_LEFT_0_SEQUENCE=GCACAGAGCCACACTGCTAG
PRIMER_RIGHT_0_SEQUENCE=TAGATTCCCATAGCCCCTCC
PRIMER_LEFT_0=50,20
PRIMER_RIGHT_0=206,20
PRIMER_LEFT_0_TM=59.801
PRIMER_RIGHT_0_TM=60.245
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=157
PRIMER_PAIR_0_PRODUCT_TM=66.5
PRIMER_PAIR_1_PENALTY=0.930867
PRIMER_LEFT_1_PENALTY=0.686256
PRIMER_RIGHT_1_PENALTY=0.244611
PRIMER_LEFT_1_SEQUENCE=CACGGAGGTCTAATGCACAG
PRIMER_RIGHT_1_SEQUENCE=TAGATTCCCATAGCCCCTCC
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=206,20
PRIMER_LEFT_1_TM=59.314
PRIMER_RIGHT_1_TM=60.245
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=171
PRIMER_PAIR_1_PRODUCT_TM=67.7
PRIMER_PAIR_2_PENALTY=1.376889
PRIMER_LEFT_2_PENALTY=0.133741
PRIMER_RIGHT_2_PENALTY=1.243148
PRIMER_LEFT_2_SEQUENCE=ACTGGCACGGAGGTCTAATG
PRIMER_RIGHT_2_SEQUENCE=AGATTCCCATAGCCCCTCC
PRIMER_LEFT_2=31,20
PRIMER_RIGHT_2=205,19
PRIMER_LEFT_2_TM=60.134
PRIMER_RIGHT_2_TM=60.243
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=175
PRIMER_PAIR_2_PRODUCT_TM=68.2
PRIMER_PAIR_3_PENALTY=1.442134
PRIMER_LEFT_3_PENALTY=0.198986
PRIMER_RIGHT_3_PENALTY=1.243148
PRIMER_LEFT_3_SEQUENCE=GCACAGAGCCACACTGCTAG
PRIMER_RIGHT_3_SEQUENCE=AGATTCCCATAGCCCCTCC
PRIMER_LEFT_3=50,20
PRIMER_RIGHT_3=205,19
PRIMER_LEFT_3_TM=59.801
PRIMER_RIGHT_3_TM=60.243
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=156
PRIMER_PAIR_3_PRODUCT_TM=66.6
PRIMER_PAIR_4_PENALTY=1.656833
PRIMER_LEFT_4_PENALTY=1.412222
PRIMER_RIGHT_4_PENALTY=0.244611
PRIMER_LEFT_4_SEQUENCE=GAGGTCTAATGCACAGAGCCA
PRIMER_RIGHT_4_SEQUENCE=TAGATTCCCATAGCCCCTCC
PRIMER_LEFT_4=40,21
PRIMER_RIGHT_4=206,20
PRIMER_LEFT_4_TM=60.412
PRIMER_RIGHT_4_TM=60.245
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=167
PRIMER_PAIR_4_PRODUCT_TM=67.1
=
SEQUENCE_ID=28.RHAP33FC4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTGTCCAACAGCCTGGCTGGATAGGTGAGGTCCAGCCTCAATATGAGATCCTGCCTCAAAAAATAAAGCATGATAGAGGAAGACACATAGACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCTCAAAATTCTCTATTCCTTTAAACATATTCTCAGTCATCAGTAAAAGAAAATTTCTATTTATTAATAAGTTAGTAGGAATTATTTTGTTGCTAGTAGATTTTGATACAGACTTGTGTTGTCCAGGTTAGTCTTAAACTGGTCTCACATATTTTACTGCAGTCTGAAGTTACAGGCCTGTGTCANTGTGNNTGGCTTACAGNATTTGCT
SEQUENCE_INCLUDED_REGION=22,359
SEQUENCE_TARGET=117,50
P3_COMMENT=CL is GGTGAGGTCCAGCCTCAATA
P3_COMMENT=CR is GGCCTGTAACTTCAGACTGCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.519602
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=1.446651
PRIMER_LEFT_0_SEQUENCE=GGTGAGGTCCAGCCTCAATA
PRIMER_RIGHT_0_SEQUENCE=GGCCTGTAACTTCAGACTGCA
PRIMER_LEFT_0=45,20
PRIMER_RIGHT_0=348,21
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=60.447
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=6.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=304
PRIMER_PAIR_0_PRODUCT_TM=69.8
PRIMER_PAIR_1_PENALTY=1.723417
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=1.650466
PRIMER_LEFT_1_SEQUENCE=GGTGAGGTCCAGCCTCAATA
PRIMER_RIGHT_1_SEQUENCE=TGACACAGGCCTGTAACTTCA
PRIMER_LEFT_1=45,20
PRIMER_RIGHT_1=355,21
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=59.350
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=311
PRIMER_PAIR_1_PRODUCT_TM=70.0
PRIMER_PAIR_2_PENALTY=1.767555
PRIMER_LEFT_2_PENALTY=0.320904
PRIMER_RIGHT_2_PENALTY=1.446651
PRIMER_LEFT_2_SEQUENCE=ATAGGTGAGGTCCAGCCTCA
PRIMER_RIGHT_2_SEQUENCE=GGCCTGTAACTTCAGACTGCA
PRIMER_LEFT_2=42,20
PRIMER_RIGHT_2=348,21
PRIMER_LEFT_2_TM=59.679
PRIMER_RIGHT_2_TM=60.447
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=307
PRIMER_PAIR_2_PRODUCT_TM=69.7
PRIMER_PAIR_3_PENALTY=1.767555
PRIMER_LEFT_3_PENALTY=0.320904
PRIMER_RIGHT_3_PENALTY=1.446651
PRIMER_LEFT_3_SEQUENCE=CTGGATAGGTGAGGTCCAGC
PRIMER_RIGHT_3_SEQUENCE=GGCCTGTAACTTCAGACTGCA
PRIMER_LEFT_3=38,20
PRIMER_RIGHT_3=348,21
PRIMER_LEFT_3_TM=59.679
PRIMER_RIGHT_3_TM=60.447
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=311
PRIMER_PAIR_3_PRODUCT_TM=70.0
PRIMER_PAIR_4_PENALTY=1.767555
PRIMER_LEFT_4_PENALTY=0.320904
PRIMER_RIGHT_4_PENALTY=1.446651
PRIMER_LEFT_4_SEQUENCE=GCTGGATAGGTGAGGTCCAG
PRIMER_RIGHT_4_SEQUENCE=GGCCTGTAACTTCAGACTGCA
PRIMER_LEFT_4=37,20
PRIMER_RIGHT_4=348,21
PRIMER_LEFT_4_TM=59.679
PRIMER_RIGHT_4_TM=60.447
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=312
PRIMER_PAIR_4_PRODUCT_TM=70.1
=
SEQUENCE_ID=28.RHAP34FC4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNACAATAATATATGAGTTGTTCCTTTAATCATGAATCATATTTCCTAGTATATTTTGAAACAATATTATTCATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATCTTCAAATGTACTGAATATTTCATCTATCACTTCTAATACTGAGGAAGCACCCAAGCACTCACCTTCAGAGAGGCAAAAGTGTTAGGGAAAGCACATTTCTTCTTCTTAAATACAT
SEQUENCE_INCLUDED_REGION=12,274
SEQUENCE_TARGET=91,72
P3_COMMENT=CL is GAGTTGTTCCTTTAATCATGAATCA
P3_COMMENT=CR is GCCTCTCTGAAGGTGAGTGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.521283
PRIMER_LEFT_0_PENALTY=6.377880
PRIMER_RIGHT_0_PENALTY=0.143403
PRIMER_LEFT_0_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
PRIMER_RIGHT_0_SEQUENCE=GCCTCTCTGAAGGTGAGTGC
PRIMER_LEFT_0=24,25
PRIMER_RIGHT_0=244,20
PRIMER_LEFT_0_TM=58.622
PRIMER_RIGHT_0_TM=60.143
PRIMER_LEFT_0_GC_PERCENT=32.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=221
PRIMER_PAIR_0_PRODUCT_TM=65.7
PRIMER_PAIR_1_PENALTY=6.521283
PRIMER_LEFT_1_PENALTY=6.377880
PRIMER_RIGHT_1_PENALTY=0.143403
PRIMER_LEFT_1_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
PRIMER_RIGHT_1_SEQUENCE=GCCTCTCTGAAGGTGAGTGC
PRIMER_LEFT_1=23,25
PRIMER_RIGHT_1=244,20
PRIMER_LEFT_1_TM=58.622
PRIMER_RIGHT_1_TM=60.143
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=222
PRIMER_PAIR_1_PRODUCT_TM=65.7
PRIMER_PAIR_2_PENALTY=6.675898
PRIMER_LEFT_2_PENALTY=6.377880
PRIMER_RIGHT_2_PENALTY=0.298018
PRIMER_LEFT_2_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
PRIMER_RIGHT_2_SEQUENCE=CTGAAGGTGAGTGCTTGGGT
PRIMER_LEFT_2=24,25
PRIMER_RIGHT_2=238,20
PRIMER_LEFT_2_TM=58.622
PRIMER_RIGHT_2_TM=60.298
PRIMER_LEFT_2_GC_PERCENT=32.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=215
PRIMER_PAIR_2_PRODUCT_TM=65.1
PRIMER_PAIR_3_PENALTY=6.675898
PRIMER_LEFT_3_PENALTY=6.377880
PRIMER_RIGHT_3_PENALTY=0.298018
PRIMER_LEFT_3_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
PRIMER_RIGHT_3_SEQUENCE=CTGAAGGTGAGTGCTTGGGT
PRIMER_LEFT_3=23,25
PRIMER_RIGHT_3=238,20
PRIMER_LEFT_3_TM=58.622
PRIMER_RIGHT_3_TM=60.298
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=216
PRIMER_PAIR_3_PRODUCT_TM=65.1
PRIMER_PAIR_4_PENALTY=6.774824
PRIMER_LEFT_4_PENALTY=6.377880
PRIMER_RIGHT_4_PENALTY=0.396944
PRIMER_LEFT_4_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
PRIMER_RIGHT_4_SEQUENCE=GCTTGGGTGCTTCCTCAGTA
PRIMER_LEFT_4=24,25
PRIMER_RIGHT_4=226,20
PRIMER_LEFT_4_TM=58.622
PRIMER_RIGHT_4_TM=60.397
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=203
PRIMER_PAIR_4_PRODUCT_TM=64.2
=
SEQUENCE_ID=28.RHAP34FG4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGGTATCCTCCCTCTTTTGTCTTAGTTTAACCAGGAGAAACTCTCCCAAACTTTAACCTTATTAACAATTGCATGCAAAGTATGCCTGACTTAGGAATGTGGTGCAAAAGTCAGTCCCAAGGCGAGGAAATAACAGGTATTTTCTGAAGTCTTGTCTTAAGTCTGTTGTTGCAGCGGCCTTGAGTTTAATCCGTGGCACTGAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGGGCAAACTGGAATCTCAGGAA
SEQUENCE_INCLUDED_REGION=20,280
SEQUENCE_TARGET=233,42
P3_COMMENT=CL is CAAGGCGAGGAAATAACAGG
P3_COMMENT=CR is TCCTGAGATTCCAGTTTGCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.493482
PRIMER_LEFT_0_PENALTY=0.298125
PRIMER_RIGHT_0_PENALTY=0.195358
PRIMER_LEFT_0_SEQUENCE=CAAGGCGAGGAAATAACAGG
PRIMER_RIGHT_0_SEQUENCE=TCCTGAGATTCCAGTTTGCC
PRIMER_LEFT_0=139,20
PRIMER_RIGHT_0=298,20
PRIMER_LEFT_0_TM=59.702
PRIMER_RIGHT_0_TM=60.195
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=160
PRIMER_PAIR_0_PRODUCT_TM=69.2
PRIMER_PAIR_1_PENALTY=0.493482
PRIMER_LEFT_1_PENALTY=0.298125
PRIMER_RIGHT_1_PENALTY=0.195358
PRIMER_LEFT_1_SEQUENCE=CCAAGGCGAGGAAATAACAG
PRIMER_RIGHT_1_SEQUENCE=TCCTGAGATTCCAGTTTGCC
PRIMER_LEFT_1=138,20
PRIMER_RIGHT_1=298,20
PRIMER_LEFT_1_TM=59.702
PRIMER_RIGHT_1_TM=60.195
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=161
PRIMER_PAIR_1_PRODUCT_TM=69.4
PRIMER_PAIR_2_PENALTY=1.144071
PRIMER_LEFT_2_PENALTY=0.948713
PRIMER_RIGHT_2_PENALTY=0.195358
PRIMER_LEFT_2_SEQUENCE=AGTCCCAAGGCGAGGAAATA
PRIMER_RIGHT_2_SEQUENCE=TCCTGAGATTCCAGTTTGCC
PRIMER_LEFT_2=134,20
PRIMER_RIGHT_2=298,20
PRIMER_LEFT_2_TM=60.949
PRIMER_RIGHT_2_TM=60.195
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=165
PRIMER_PAIR_2_PRODUCT_TM=69.7
PRIMER_PAIR_3_PENALTY=1.216668
PRIMER_LEFT_3_PENALTY=1.021310
PRIMER_RIGHT_3_PENALTY=0.195358
PRIMER_LEFT_3_SEQUENCE=GTCCCAAGGCGAGGAAATA
PRIMER_RIGHT_3_SEQUENCE=TCCTGAGATTCCAGTTTGCC
PRIMER_LEFT_3=135,19
PRIMER_RIGHT_3=298,20
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=60.195
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=164
PRIMER_PAIR_3_PRODUCT_TM=69.7
PRIMER_PAIR_4_PENALTY=1.399259
PRIMER_LEFT_4_PENALTY=1.203901
PRIMER_RIGHT_4_PENALTY=0.195358
PRIMER_LEFT_4_SEQUENCE=TGCAAAAGTCAGTCCCAAGG
PRIMER_RIGHT_4_SEQUENCE=TCCTGAGATTCCAGTTTGCC
PRIMER_LEFT_4=124,20
PRIMER_RIGHT_4=298,20
PRIMER_LEFT_4_TM=61.204
PRIMER_RIGHT_4_TM=60.195
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=175
PRIMER_PAIR_4_PRODUCT_TM=70.1
=
SEQUENCE_ID=29.RHAP33FG5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTAATGCAGCATTGATTTCTGAAGNNNNNNNNNNNNNNATTTGGGCTTCTGATAATTGTTTGGTTTGCCTATTGCCTTAGAATTAGTGTCGATTACGACCAACAGGAAAACTTCAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTTTCNCACATGGACCTNTTTGTTTGAAAGTCCCTGCNGCTGCTTTGGACTGTGTGGATANNGTGCTCCACCTAGNCAGTTTGTGTGTATGGCATGNATATGCANCACACACACCGTGGTGCNTGGNTGNCCTGTGNAGGANAANAGCAGGGATNTTANATT
SEQUENCE_INCLUDED_REGION=14,371
SEQUENCE_TARGET=38,14
SEQUENCE_TARGET=133,87
P3_COMMENT=CL is CGACCAACAGGAAAACTTCAA
P3_COMMENT=CR is ATCCACACAGTCCAAAGCAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.407334
PRIMER_LEFT_0_PENALTY=1.131978
PRIMER_RIGHT_0_PENALTY=1.275356
PRIMER_LEFT_0_SEQUENCE=CGACCAACAGGAAAACTTCAA
PRIMER_RIGHT_0_SEQUENCE=ATCCACACAGTCCAAAGCAG
PRIMER_LEFT_0=109,21
PRIMER_RIGHT_0=281,20
PRIMER_LEFT_0_TM=60.132
PRIMER_RIGHT_0_TM=58.725
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=173
PRIMER_PAIR_0_PRODUCT_TM=65.4
PRIMER_PAIR_1_PENALTY=2.529067
PRIMER_LEFT_1_PENALTY=1.253711
PRIMER_RIGHT_1_PENALTY=1.275356
PRIMER_LEFT_1_SEQUENCE=CGACCAACAGGAAAACTTCA
PRIMER_RIGHT_1_SEQUENCE=ATCCACACAGTCCAAAGCAG
PRIMER_LEFT_1=109,20
PRIMER_RIGHT_1=281,20
PRIMER_LEFT_1_TM=58.746
PRIMER_RIGHT_1_TM=58.725
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=173
PRIMER_PAIR_1_PRODUCT_TM=65.4
PRIMER_PAIR_2_PENALTY=2.588584
PRIMER_LEFT_2_PENALTY=1.131978
PRIMER_RIGHT_2_PENALTY=1.456605
PRIMER_LEFT_2_SEQUENCE=CGACCAACAGGAAAACTTCAA
PRIMER_RIGHT_2_SEQUENCE=CCACACAGTCCAAAGCAGC
PRIMER_LEFT_2=109,21
PRIMER_RIGHT_2=279,19
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.457
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=65.5
PRIMER_PAIR_3_PENALTY=2.650983
PRIMER_LEFT_3_PENALTY=1.375627
PRIMER_RIGHT_3_PENALTY=1.275356
PRIMER_LEFT_3_SEQUENCE=ACGACCAACAGGAAAACTTCA
PRIMER_RIGHT_3_SEQUENCE=ATCCACACAGTCCAAAGCAG
PRIMER_LEFT_3=108,21
PRIMER_RIGHT_3=281,20
PRIMER_LEFT_3_TM=59.624
PRIMER_RIGHT_3_TM=58.725
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=65.4
PRIMER_PAIR_4_PENALTY=2.710317
PRIMER_LEFT_4_PENALTY=1.253711
PRIMER_RIGHT_4_PENALTY=1.456605
PRIMER_LEFT_4_SEQUENCE=CGACCAACAGGAAAACTTCA
PRIMER_RIGHT_4_SEQUENCE=CCACACAGTCCAAAGCAGC
PRIMER_LEFT_4=109,20
PRIMER_RIGHT_4=279,19
PRIMER_LEFT_4_TM=58.746
PRIMER_RIGHT_4_TM=60.457
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=171
PRIMER_PAIR_4_PRODUCT_TM=65.5
=
SEQUENCE_ID=30.RHAP34FC6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNACCCACACGGGCACATTCAGCCGCANANATGAGNCNCAGNCGCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNACAGCAACTTCTCACCAAACNATCCAAAACCTACNGACTTGAGGGGCNGTCCTTGTCGTAGCACTAAANGCANANGGCAGCAGGTGGACACTGGTGGTGTGGGCACCTTGNGTNNGAGAGNCGGGGGNNANGAGGNCCNGGAGGGGNGNNACCGANGCNTCGGNAAGNTTCGNNAANNCANNGGNTCANNAGNCGNGGNNNNCCNNGGGGGGGGNGNA
SEQUENCE_INCLUDED_REGION=12,314
SEQUENCE_TARGET=58,18
P3_COMMENT=CL is CCACACGGGCACATTCAG
P3_COMMENT=CR is CACACCACCAGTGTCCACC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.172776
PRIMER_LEFT_0_PENALTY=4.227928
PRIMER_RIGHT_0_PENALTY=1.944848
PRIMER_LEFT_0_SEQUENCE=CCACACGGGCACATTCAG
PRIMER_RIGHT_0_SEQUENCE=CACACCACCAGTGTCCACC
PRIMER_LEFT_0=14,18
PRIMER_RIGHT_0=208,19
PRIMER_LEFT_0_TM=62.228
PRIMER_RIGHT_0_TM=60.945
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=195
PRIMER_PAIR_0_PRODUCT_TM=72.6
PRIMER_PAIR_1_PENALTY=6.172776
PRIMER_LEFT_1_PENALTY=4.227928
PRIMER_RIGHT_1_PENALTY=1.944848
PRIMER_LEFT_1_SEQUENCE=CCACACGGGCACATTCAG
PRIMER_RIGHT_1_SEQUENCE=CCACACCACCAGTGTCCAC
PRIMER_LEFT_1=14,18
PRIMER_RIGHT_1=209,19
PRIMER_LEFT_1_TM=62.228
PRIMER_RIGHT_1_TM=60.945
PRIMER_LEFT_1_GC_PERCENT=61.111
PRIMER_RIGHT_1_GC_PERCENT=63.158
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=196
PRIMER_PAIR_1_PRODUCT_TM=72.7
PRIMER_PAIR_2_PENALTY=6.203441
PRIMER_LEFT_2_PENALTY=4.227928
PRIMER_RIGHT_2_PENALTY=1.975514
PRIMER_LEFT_2_SEQUENCE=CCACACGGGCACATTCAG
PRIMER_RIGHT_2_SEQUENCE=ACACCACCAGTGTCCACCTG
PRIMER_LEFT_2=14,18
PRIMER_RIGHT_2=207,20
PRIMER_LEFT_2_TM=62.228
PRIMER_RIGHT_2_TM=61.976
PRIMER_LEFT_2_GC_PERCENT=61.111
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=194
PRIMER_PAIR_2_PRODUCT_TM=72.4
PRIMER_PAIR_3_PENALTY=6.203441
PRIMER_LEFT_3_PENALTY=4.227928
PRIMER_RIGHT_3_PENALTY=1.975514
PRIMER_LEFT_3_SEQUENCE=CCACACGGGCACATTCAG
PRIMER_RIGHT_3_SEQUENCE=CACACCACCAGTGTCCACCT
PRIMER_LEFT_3=14,18
PRIMER_RIGHT_3=208,20
PRIMER_LEFT_3_TM=62.228
PRIMER_RIGHT_3_TM=61.976
PRIMER_LEFT_3_GC_PERCENT=61.111
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=195
PRIMER_PAIR_3_PRODUCT_TM=72.6
PRIMER_PAIR_4_PENALTY=6.268774
PRIMER_LEFT_4_PENALTY=4.227928
PRIMER_RIGHT_4_PENALTY=2.040846
PRIMER_LEFT_4_SEQUENCE=CCACACGGGCACATTCAG
PRIMER_RIGHT_4_SEQUENCE=CCACACCACCAGTGTCCA
PRIMER_LEFT_4=14,18
PRIMER_RIGHT_4=209,18
PRIMER_LEFT_4_TM=62.228
PRIMER_RIGHT_4_TM=59.959
PRIMER_LEFT_4_GC_PERCENT=61.111
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=196
PRIMER_PAIR_4_PRODUCT_TM=72.7
=
SEQUENCE_ID=32.RHAP33FG8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAGATGACAGGGTGCCCGCCTCCTGTGGGCGCAGGTGTGAGTCCTTGTAAGGCAACCTTGGGTTCCTGGCTGGACCTCAGAGGTTTCCCTCCATTGAGTGTGAGAGGGACTGTGGTAGTGGGGAAGGAACTGGTGGGCAGTGGAGGGTTCGCTCTTTAGACAGAGTACTACATACATAGAGAGTAAAGCAAAGAGCCGTAAAGAGGTGTGTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTTGTGTGGGTACACACATGCATGCAGACCTGANGCCNTCNNCAATTACNTNC
SEQUENCE_INCLUDED_REGION=29,362
SEQUENCE_TARGET=248,35
SEQUENCE_TARGET=288,48
P3_COMMENT=CL is AAGCAAAGAGCCGTAAAGAGG
P3_COMMENT=CR is AGGTCTGCATGCATGTGTGT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.223834
PRIMER_LEFT_0_PENALTY=1.028128
PRIMER_RIGHT_0_PENALTY=0.195706
PRIMER_LEFT_0_SEQUENCE=AAGCAAAGAGCCGTAAAGAGG
PRIMER_RIGHT_0_SEQUENCE=AGGTCTGCATGCATGTGTGT
PRIMER_LEFT_0=217,21
PRIMER_RIGHT_0=368,20
PRIMER_LEFT_0_TM=60.028
PRIMER_RIGHT_0_TM=60.196
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=152
PRIMER_PAIR_0_PRODUCT_TM=64.3
PRIMER_PAIR_1_PENALTY=1.452864
PRIMER_LEFT_1_PENALTY=1.257158
PRIMER_RIGHT_1_PENALTY=0.195706
PRIMER_LEFT_1_SEQUENCE=AGCAAAGAGCCGTAAAGAGG
PRIMER_RIGHT_1_SEQUENCE=AGGTCTGCATGCATGTGTGT
PRIMER_LEFT_1=218,20
PRIMER_RIGHT_1=368,20
PRIMER_LEFT_1_TM=58.743
PRIMER_RIGHT_1_TM=60.196
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=151
PRIMER_PAIR_1_PRODUCT_TM=64.3
PRIMER_PAIR_2_PENALTY=1.591782
PRIMER_LEFT_2_PENALTY=1.396076
PRIMER_RIGHT_2_PENALTY=0.195706
PRIMER_LEFT_2_SEQUENCE=GCAAAGAGCCGTAAAGAGGT
PRIMER_RIGHT_2_SEQUENCE=AGGTCTGCATGCATGTGTGT
PRIMER_LEFT_2=219,20
PRIMER_RIGHT_2=368,20
PRIMER_LEFT_2_TM=58.604
PRIMER_RIGHT_2_TM=60.196
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_2_PRODUCT_TM=64.4
PRIMER_PAIR_3_PENALTY=1.639290
PRIMER_LEFT_3_PENALTY=1.443584
PRIMER_RIGHT_3_PENALTY=0.195706
PRIMER_LEFT_3_SEQUENCE=AGCAAAGAGCCGTAAAGAGGT
PRIMER_RIGHT_3_SEQUENCE=AGGTCTGCATGCATGTGTGT
PRIMER_LEFT_3=218,21
PRIMER_RIGHT_3=368,20
PRIMER_LEFT_3_TM=59.556
PRIMER_RIGHT_3_TM=60.196
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=151
PRIMER_PAIR_3_PRODUCT_TM=64.3
PRIMER_PAIR_4_PENALTY=1.900512
PRIMER_LEFT_4_PENALTY=1.704806
PRIMER_RIGHT_4_PENALTY=0.195706
PRIMER_LEFT_4_SEQUENCE=AGCCGTAAAGAGGTGTGTTTG
PRIMER_RIGHT_4_SEQUENCE=AGGTCTGCATGCATGTGTGT
PRIMER_LEFT_4=225,21
PRIMER_RIGHT_4=368,20
PRIMER_LEFT_4_TM=59.295
PRIMER_RIGHT_4_TM=60.196
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=144
PRIMER_PAIR_4_PRODUCT_TM=63.7
=
SEQUENCE_ID=33.RHAP33FG9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTAGTCCAGTCAAATGGATGATCACTAGGTGCAAAGGGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGANAACAGCNTCCACCTAGAGCACNNNNNNNNNNNNNNACNAAGNAAACACATTTTCATTCCACACTTACATCATGCACACACATGCNTGCNGTGCATGNNNNNNNNNNNNNNNNCCCCCCCCANATAACNATAAAACCATACAGCAAACATNNTGTACAACCACCACNATNNNNNNNNNNNNNNTTTGTNTTCCGNTGGGCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=15,312
SEQUENCE_TARGET=59,58
SEQUENCE_TARGET=145,14
SEQUENCE_TARGET=220,16
SEQUENCE_TARGET=292,14
P3_COMMENT=CL is GATCACTAGGTGCAAAGGGG
P3_COMMENT=CR is GCATGTGTGTGCATGATGTAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.437392
PRIMER_LEFT_0_PENALTY=0.449829
PRIMER_RIGHT_0_PENALTY=1.987562
PRIMER_LEFT_0_SEQUENCE=GATCACTAGGTGCAAAGGGG
PRIMER_RIGHT_0_SEQUENCE=GCATGTGTGTGCATGATGTAA
PRIMER_LEFT_0=34,20
PRIMER_RIGHT_0=207,21
PRIMER_LEFT_0_TM=59.550
PRIMER_RIGHT_0_TM=59.012
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=67.5
PRIMER_PAIR_1_PENALTY=2.484366
PRIMER_LEFT_1_PENALTY=0.449829
PRIMER_RIGHT_1_PENALTY=2.034537
PRIMER_LEFT_1_SEQUENCE=GATCACTAGGTGCAAAGGGG
PRIMER_RIGHT_1_SEQUENCE=TGTGCATGATGTAAGTGTGGAA
PRIMER_LEFT_1=34,20
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=59.550
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=167
PRIMER_PAIR_1_PRODUCT_TM=66.9
PRIMER_PAIR_2_PENALTY=2.515032
PRIMER_LEFT_2_PENALTY=0.449829
PRIMER_RIGHT_2_PENALTY=2.065202
PRIMER_LEFT_2_SEQUENCE=GATCACTAGGTGCAAAGGGG
PRIMER_RIGHT_2_SEQUENCE=GCATGTGTGTGCATGATGTAAG
PRIMER_LEFT_2=34,20
PRIMER_RIGHT_2=207,22
PRIMER_LEFT_2_TM=59.550
PRIMER_RIGHT_2_TM=60.065
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=174
PRIMER_PAIR_2_PRODUCT_TM=67.5
PRIMER_PAIR_3_PENALTY=2.892209
PRIMER_LEFT_3_PENALTY=0.449829
PRIMER_RIGHT_3_PENALTY=2.442380
PRIMER_LEFT_3_SEQUENCE=GATCACTAGGTGCAAAGGGG
PRIMER_RIGHT_3_SEQUENCE=TGTGCATGATGTAAGTGTGGA
PRIMER_LEFT_3=34,20
PRIMER_RIGHT_3=200,21
PRIMER_LEFT_3_TM=59.550
PRIMER_RIGHT_3_TM=58.558
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=66.9
PRIMER_PAIR_4_PENALTY=2.944233
PRIMER_LEFT_4_PENALTY=0.449829
PRIMER_RIGHT_4_PENALTY=2.494404
PRIMER_LEFT_4_SEQUENCE=GATCACTAGGTGCAAAGGGG
PRIMER_RIGHT_4_SEQUENCE=TGTGTGCATGATGTAAGTGTGG
PRIMER_LEFT_4=34,20
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=59.550
PRIMER_RIGHT_4_TM=60.494
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=169
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=34.RHAP33FG10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTATTTTGATCTCCATATGTTTTATTTCTATATCTATTCCTAATAGTTTTTANNNNNNNNNNNNNNNNGCATGCANNNNNNNNNNNNNNNNNNNNNNNNTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNTTGCTTAAAGATCAAGAAAGATGAGTTGTACCTGAGAAAACTTGCTAAGTCCCCCACTAGTAGTGGATCGACAATTAGACATTTTCCCATCAAGGACTGAAGCGTCTTTCTTGCGGACAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=17,254
SEQUENCE_TARGET=69,16
SEQUENCE_TARGET=92,24
SEQUENCE_TARGET=122,26
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=35.RHAP33FG11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGACCACTGCGTCTCAGGAAATAAGGTGAAAAGAAACTGAGGAAGACAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCACTCACACAAGTTTTCTCAATTCCAGGCTATTTCCCCTTAGCGATAAGTGTAAGTTCATTCAAGAGCAGATGCTATTTTTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=27,174
SEQUENCE_TARGET=83,30
P3_COMMENT=CL is ACCACTGCGTCTCAGGAAAT
P3_COMMENT=CR is GCTAAGGGGAAATAGCCTGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.327181
PRIMER_LEFT_0_PENALTY=0.272667
PRIMER_RIGHT_0_PENALTY=0.054514
PRIMER_LEFT_0_SEQUENCE=ACCACTGCGTCTCAGGAAAT
PRIMER_RIGHT_0_SEQUENCE=GCTAAGGGGAAATAGCCTGG
PRIMER_LEFT_0=32,20
PRIMER_RIGHT_0=158,20
PRIMER_LEFT_0_TM=59.727
PRIMER_RIGHT_0_TM=60.055
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=127
PRIMER_PAIR_0_PRODUCT_TM=70.0
PRIMER_PAIR_1_PENALTY=0.456501
PRIMER_LEFT_1_PENALTY=0.272667
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=ACCACTGCGTCTCAGGAAAT
PRIMER_RIGHT_1_SEQUENCE=TCGCTAAGGGGAAATAGCCT
PRIMER_LEFT_1=32,20
PRIMER_RIGHT_1=160,20
PRIMER_LEFT_1_TM=59.727
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=129
PRIMER_PAIR_1_PRODUCT_TM=70.2
PRIMER_PAIR_2_PENALTY=0.662598
PRIMER_LEFT_2_PENALTY=0.608084
PRIMER_RIGHT_2_PENALTY=0.054514
PRIMER_LEFT_2_SEQUENCE=AGAGACCACTGCGTCTCAGG
PRIMER_RIGHT_2_SEQUENCE=GCTAAGGGGAAATAGCCTGG
PRIMER_LEFT_2=28,20
PRIMER_RIGHT_2=158,20
PRIMER_LEFT_2_TM=60.608
PRIMER_RIGHT_2_TM=60.055
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=131
PRIMER_PAIR_2_PRODUCT_TM=70.3
PRIMER_PAIR_3_PENALTY=0.717627
PRIMER_LEFT_3_PENALTY=0.272667
PRIMER_RIGHT_3_PENALTY=0.444960
PRIMER_LEFT_3_SEQUENCE=ACCACTGCGTCTCAGGAAAT
PRIMER_RIGHT_3_SEQUENCE=ATCGCTAAGGGGAAATAGCC
PRIMER_LEFT_3=32,20
PRIMER_RIGHT_3=161,20
PRIMER_LEFT_3_TM=59.727
PRIMER_RIGHT_3_TM=59.555
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=130
PRIMER_PAIR_3_PRODUCT_TM=70.1
PRIMER_PAIR_4_PENALTY=0.791919
PRIMER_LEFT_4_PENALTY=0.608084
PRIMER_RIGHT_4_PENALTY=0.183834
PRIMER_LEFT_4_SEQUENCE=AGAGACCACTGCGTCTCAGG
PRIMER_RIGHT_4_SEQUENCE=TCGCTAAGGGGAAATAGCCT
PRIMER_LEFT_4=28,20
PRIMER_RIGHT_4=160,20
PRIMER_LEFT_4_TM=60.608
PRIMER_RIGHT_4_TM=60.184
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=9.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=133
PRIMER_PAIR_4_PRODUCT_TM=70.5
=
SEQUENCE_ID=36.RHAP33FG12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTTCTGTTTAGATTGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGGTTTCACAGGCTGGCTATTCATGCTTCATGGTCTTCAGTTCTGGGATAACAGATATGGCCACAAGTGGCTTACTTTAAAGTTTTGACATTAACAAACATTTTTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=17,174
SEQUENCE_TARGET=37,44
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=36.RHAP34FG12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCAGGTTATGCAAGGCTGGTTGGGTGCAGGGCTAAGAGATGCTGAAAAAAAAAGTGTATCTACAATTAGATTATTTCATTTCATTTGCTGTGGAGAATCTACAGGTGTGTGAGTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAACTACTCTTGGGATAACATGTGGAAGAGTCTGGGTGTGGAAAGCTGTCATTCGGGAAAGAAAAATAAGAGCACTGTGATCCCAGGTACAAACATTTTAGAGGCAGATTTTAGGGNNNN
SEQUENCE_INCLUDED_REGION=19,276
SEQUENCE_TARGET=137,36
P3_COMMENT=CL is GCAGGGCTAAGAGATGCTGA
P3_COMMENT=CR is CACACCCAGACTCTTCCACA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.940817
PRIMER_LEFT_0_PENALTY=0.645726
PRIMER_RIGHT_0_PENALTY=0.295091
PRIMER_LEFT_0_SEQUENCE=GCAGGGCTAAGAGATGCTGA
PRIMER_RIGHT_0_SEQUENCE=CACACCCAGACTCTTCCACA
PRIMER_LEFT_0=44,20
PRIMER_RIGHT_0=218,20
PRIMER_LEFT_0_TM=60.646
PRIMER_RIGHT_0_TM=59.705
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=175
PRIMER_PAIR_0_PRODUCT_TM=69.6
PRIMER_PAIR_1_PENALTY=1.299667
PRIMER_LEFT_1_PENALTY=0.396940
PRIMER_RIGHT_1_PENALTY=0.902727
PRIMER_LEFT_1_SEQUENCE=GTGCAGGGCTAAGAGATGCT
PRIMER_RIGHT_1_SEQUENCE=CACCCAGACTCTTCCACATG
PRIMER_LEFT_1=42,20
PRIMER_RIGHT_1=216,20
PRIMER_LEFT_1_TM=59.603
PRIMER_RIGHT_1_TM=59.097
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=69.6
PRIMER_PAIR_2_PENALTY=1.414540
PRIMER_LEFT_2_PENALTY=0.396940
PRIMER_RIGHT_2_PENALTY=1.017601
PRIMER_LEFT_2_SEQUENCE=GTGCAGGGCTAAGAGATGCT
PRIMER_RIGHT_2_SEQUENCE=CACCCAGACTCTTCCACATGT
PRIMER_LEFT_2=42,20
PRIMER_RIGHT_2=216,21
PRIMER_LEFT_2_TM=59.603
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=175
PRIMER_PAIR_2_PRODUCT_TM=69.6
PRIMER_PAIR_3_PENALTY=1.548453
PRIMER_LEFT_3_PENALTY=0.645726
PRIMER_RIGHT_3_PENALTY=0.902727
PRIMER_LEFT_3_SEQUENCE=GCAGGGCTAAGAGATGCTGA
PRIMER_RIGHT_3_SEQUENCE=CACCCAGACTCTTCCACATG
PRIMER_LEFT_3=44,20
PRIMER_RIGHT_3=216,20
PRIMER_LEFT_3_TM=60.646
PRIMER_RIGHT_3_TM=59.097
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=173
PRIMER_PAIR_3_PRODUCT_TM=69.5
PRIMER_PAIR_4_PENALTY=1.663327
PRIMER_LEFT_4_PENALTY=0.645726
PRIMER_RIGHT_4_PENALTY=1.017601
PRIMER_LEFT_4_SEQUENCE=GCAGGGCTAAGAGATGCTGA
PRIMER_RIGHT_4_SEQUENCE=CACCCAGACTCTTCCACATGT
PRIMER_LEFT_4=44,20
PRIMER_RIGHT_4=216,21
PRIMER_LEFT_4_TM=60.646
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=173
PRIMER_PAIR_4_PRODUCT_TM=69.5
=
SEQUENCE_ID=37.RHAP33FD1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNCTTCACTTTCATACATGGAAGTCACTATCTCAGAGCAGATACCCTGGTCCTCTGTCCCTTACAATCTCTCCGTGCTATCTTTCAGAGATGTTCCCTAAGCCTTAGGTGTAGGGGTTGTGTTGAAGATGCACCAAGGATAAGGATTCAGTCAGTTGTCTGCGTTTTTTTTTACCAGTTCTGAGTTTCTCTGATACCCTTTATCTATATGCAGCAGCTGGCAGCAGTATGTACTGTTGGCAAACTGATAAAATGTTCTGGATCTGTAATGAAAGACACACATATAGATAAGTATATANACATNNNNNNNNNNNNNNNNAAGNGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCA
SEQUENCE_INCLUDED_REGION=10,361
SEQUENCE_TARGET=310,16
SEQUENCE_TARGET=333,33
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=37.RHAP33FH1.seq
SEQUENCE_TEMPLATE=NNNNTCCCGGTACGGGCGCGCCCNCCCCCGNNNNNNNNNNNNNNNNNNNNNTGTGGNGCGCGTTTGTCGGNTTTGNGTCCAGAGGCTGTTGTTAGGTATGGAGGGNGTGGAGGNGGAGGGTGTTCTTGGTGCCAGTGTGTGTNCGCNGCTGTTGGCGTTGNNNTTTGNGGGNGTNTGANGACCAANTGGCCNACGGTGCTGTGACATCAACCAGCTGGCAAAGNAAGNAGGNGCNAAGAGCGCAGCCTTCCCANGCCNNCACNGCNNNACCTCCTGCNNNTCTCCTAACCCCNNNCNCCCNCTTCNGCCTNCNCCTGNNCCTCCCTCAAGGCCGCCNNCCCNNTGTCCNNCNNCCCGCCCNNCNGGAANAGCCCCTCCACCCCCNNCNGCCNCACCNNCAATCCTCCNCNTNAACCCNCTGCCNCCT
SEQUENCE_INCLUDED_REGION=4,423
SEQUENCE_TARGET=30,21
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=37.RHAP34FD1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTACAAGGNNNNNNNNNNNNNNNNNNNATTCATATATACAGCACACGCATACAAATNNNNNNNNNNNNNNNNNNNNAACAAACATACTTACTTACACACCACACAGACCCACTACGAACTCACACAAACATTCTACATAGACACATCACATACATACCATGCAGTNNNNNNNNNNNNNNNNNNCTCATACCACACCCAATACACACACACACGTCTACTCTCTACTTCGTCNACTATCGACNACTACACACACNACNNCATCTATCTACNGCATTTACTTTACATACAAAGTTATACAGTAGNAGTAGAGTGNACCGATGACTCNGA
SEQUENCE_INCLUDED_REGION=18,328
SEQUENCE_TARGET=27,19
SEQUENCE_TARGET=75,20
SEQUENCE_TARGET=184,18
P3_COMMENT=CL is ACTTACACACCACACAGACCC
P3_COMMENT=CR is GACGTGTGTGTGTGTATTGGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.820251
PRIMER_LEFT_0_PENALTY=3.605542
PRIMER_RIGHT_0_PENALTY=1.214710
PRIMER_LEFT_0_SEQUENCE=ACTTACACACCACACAGACCC
PRIMER_RIGHT_0_SEQUENCE=GACGTGTGTGTGTGTATTGGG
PRIMER_LEFT_0=108,21
PRIMER_RIGHT_0=233,21
PRIMER_LEFT_0_TM=57.394
PRIMER_RIGHT_0_TM=59.785
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=10.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=126
PRIMER_PAIR_0_PRODUCT_TM=70.8
PRIMER_PAIR_1_PENALTY=5.267892
PRIMER_LEFT_1_PENALTY=4.053183
PRIMER_RIGHT_1_PENALTY=1.214710
PRIMER_LEFT_1_SEQUENCE=TTACTTACACACCACACAGACCC
PRIMER_RIGHT_1_SEQUENCE=GACGTGTGTGTGTGTATTGGG
PRIMER_LEFT_1=106,23
PRIMER_RIGHT_1=233,21
PRIMER_LEFT_1_TM=58.947
PRIMER_RIGHT_1_TM=59.785
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=10.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PAIR_1_PRODUCT_SIZE=128
PRIMER_PAIR_1_PRODUCT_TM=70.6
PRIMER_PAIR_2_PENALTY=5.325102
PRIMER_LEFT_2_PENALTY=4.110393
PRIMER_RIGHT_2_PENALTY=1.214710
PRIMER_LEFT_2_SEQUENCE=CTTACTTACACACCACACAGACCC
PRIMER_RIGHT_2_SEQUENCE=GACGTGTGTGTGTGTATTGGG
PRIMER_LEFT_2=105,24
PRIMER_RIGHT_2=233,21
PRIMER_LEFT_2_TM=59.890
PRIMER_RIGHT_2_TM=59.785
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=10.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=129
PRIMER_PAIR_2_PRODUCT_TM=70.8
PRIMER_PAIR_3_PENALTY=5.705479
PRIMER_LEFT_3_PENALTY=4.490770
PRIMER_RIGHT_3_PENALTY=1.214710
PRIMER_LEFT_3_SEQUENCE=TACTTACACACCACACAGACCC
PRIMER_RIGHT_3_SEQUENCE=GACGTGTGTGTGTGTATTGGG
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=233,21
PRIMER_LEFT_3_TM=57.509
PRIMER_RIGHT_3_TM=59.785
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=10.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=70.7
PRIMER_PAIR_4_PENALTY=5.773868
PRIMER_LEFT_4_PENALTY=0.063544
PRIMER_RIGHT_4_PENALTY=5.710325
PRIMER_LEFT_4_SEQUENCE=CACCACACAGACCCACTACG
PRIMER_RIGHT_4_SEQUENCE=CGAAGTAGAGAGTAGACGTGTGTG
PRIMER_LEFT_4=115,20
PRIMER_RIGHT_4=247,24
PRIMER_LEFT_4_TM=60.064
PRIMER_RIGHT_4_TM=58.290
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=133
PRIMER_PAIR_4_PRODUCT_TM=71.4
=
SEQUENCE_ID=37.RHAP34FH1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCTGCATCTCCATACTCCATTGATGTAGTGCTAGGGACAGAATTCAGGGCATCATGCATGCTACAAACTAAGCTACATCCCTAGCCCCACATGTGAAGTCTTTTGTAACTGATGTCTACTGATTTGAACCTAAGGAGGCTATAAGAGAAGAGAACCACTGAACTCCATGAGATTTAGAACAGGAAATGGAATGGAGACTATTATTTTTTCCACTGTTTCTCTGTTTCTCTGTGTTTCTCTTANNNNNNNNNNNNNNNNNNNNTCTGTCCTGTTTAAGTGTGGA
SEQUENCE_INCLUDED_REGION=18,283
SEQUENCE_TARGET=260,20
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=38.RHAP34FH2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGGGTTCGGTCCCCAGCTCCGGGGGGAAAAAAAAAAAAGAATTGCAGGTCAGTGTCATACAGGTCTTGGTGGTCTGTTTTCTTTAGTCTGTTTTATTCCATGCTGTCACACAGTAAAGATGCAGTCTACTACTGAGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATCCACATGCCATAGAATGTGTGTTGGAGGTCTGAAGAATAGCTTTCAGAGAGTTGGTTCATCATCTTTCATCATATTTTTTTTCCCCAGAAATTG
SEQUENCE_INCLUDED_REGION=20,282
SEQUENCE_TARGET=162,40
P3_COMMENT=CL is TGCAGGTCAGTGTCATACAGG
P3_COMMENT=CR is TCCAACACACATTCTATGGCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.259669
PRIMER_LEFT_0_PENALTY=1.239519
PRIMER_RIGHT_0_PENALTY=1.020151
PRIMER_LEFT_0_SEQUENCE=TGCAGGTCAGTGTCATACAGG
PRIMER_RIGHT_0_SEQUENCE=TCCAACACACATTCTATGGCA
PRIMER_LEFT_0=64,21
PRIMER_RIGHT_0=233,21
PRIMER_LEFT_0_TM=59.760
PRIMER_RIGHT_0_TM=59.980
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=170
PRIMER_PAIR_0_PRODUCT_TM=69.6
PRIMER_PAIR_1_PENALTY=2.922717
PRIMER_LEFT_1_PENALTY=1.902566
PRIMER_RIGHT_1_PENALTY=1.020151
PRIMER_LEFT_1_SEQUENCE=CAGGTCTTGGTGGTCTGTTTT
PRIMER_RIGHT_1_SEQUENCE=TCCAACACACATTCTATGGCA
PRIMER_LEFT_1=81,21
PRIMER_RIGHT_1=233,21
PRIMER_LEFT_1_TM=59.097
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=153
PRIMER_PAIR_1_PRODUCT_TM=68.6
PRIMER_PAIR_2_PENALTY=3.048207
PRIMER_LEFT_2_PENALTY=2.028056
PRIMER_RIGHT_2_PENALTY=1.020151
PRIMER_LEFT_2_SEQUENCE=TCATACAGGTCTTGGTGGTCTG
PRIMER_RIGHT_2_SEQUENCE=TCCAACACACATTCTATGGCA
PRIMER_LEFT_2=76,22
PRIMER_RIGHT_2=233,21
PRIMER_LEFT_2_TM=60.028
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=68.6
PRIMER_PAIR_3_PENALTY=3.082112
PRIMER_LEFT_3_PENALTY=2.061961
PRIMER_RIGHT_3_PENALTY=1.020151
PRIMER_LEFT_3_SEQUENCE=ACAGGTCTTGGTGGTCTGTTTT
PRIMER_RIGHT_3_SEQUENCE=TCCAACACACATTCTATGGCA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=233,21
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=59.980
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=154
PRIMER_PAIR_3_PRODUCT_TM=68.5
PRIMER_PAIR_4_PENALTY=3.129351
PRIMER_LEFT_4_PENALTY=2.109200
PRIMER_RIGHT_4_PENALTY=1.020151
PRIMER_LEFT_4_SEQUENCE=TGTCATACAGGTCTTGGTGGTC
PRIMER_RIGHT_4_SEQUENCE=TCCAACACACATTCTATGGCA
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=233,21
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=160
PRIMER_PAIR_4_PRODUCT_TM=68.7
=
SEQUENCE_ID=39.RHAP33FD3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTTTTTGGAACACAAGTGNNNNNNNNNNNNNNNNNNNNAATCCTTTTAAGCCTTTTTAAAGAAATGATTGACATTGTGAATTAGACAATTTTCAGTTATAAATTTTCAGCACACAGCACAGAAAGCCTAAATGTGCAATAAAATGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,148
SEQUENCE_TARGET=39,20
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=39.RHAP33FH3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNTATTTCTCTGTCTTTTCCTTCTACGTGCTTTCTTTCTTTTTCCCATCTCCCTAAACTCAACCAGGATTTACAGGAGAGAGGGACTTTACCATCTNNNNNNNNNNNNNNNNNNNNCCCACTGCACTCAAGCGCTCGCACGGTGCCCCTCCTCACAGCCTGTGTGCTTTGCTCTCTTCATATATGATTGACAGGGAGAAAAAAAATGAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=14,211
SEQUENCE_TARGET=108,20
P3_COMMENT=CL is CCCATCTCCCTAAACTCAACC
P3_COMMENT=CR is GAGAGCAAAGCACACAGGCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.930203
PRIMER_LEFT_0_PENALTY=1.187326
PRIMER_RIGHT_0_PENALTY=0.742877
PRIMER_LEFT_0_SEQUENCE=CCCATCTCCCTAAACTCAACC
PRIMER_RIGHT_0_SEQUENCE=GAGAGCAAAGCACACAGGCT
PRIMER_LEFT_0=55,21
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.813
PRIMER_RIGHT_0_TM=60.743
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=74.9
PRIMER_PAIR_1_PENALTY=1.930203
PRIMER_LEFT_1_PENALTY=1.187326
PRIMER_RIGHT_1_PENALTY=0.742877
PRIMER_LEFT_1_SEQUENCE=CCCATCTCCCTAAACTCAACC
PRIMER_RIGHT_1_SEQUENCE=AGAGAGCAAAGCACACAGGC
PRIMER_LEFT_1=55,21
PRIMER_RIGHT_1=187,20
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=60.743
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=133
PRIMER_PAIR_1_PRODUCT_TM=74.8
PRIMER_PAIR_2_PENALTY=1.966036
PRIMER_LEFT_2_PENALTY=1.223158
PRIMER_RIGHT_2_PENALTY=0.742877
PRIMER_LEFT_2_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC
PRIMER_RIGHT_2_SEQUENCE=GAGAGCAAAGCACACAGGCT
PRIMER_LEFT_2=37,21
PRIMER_RIGHT_2=186,20
PRIMER_LEFT_2_TM=60.223
PRIMER_RIGHT_2_TM=60.743
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_2_PRODUCT_TM=74.8
PRIMER_PAIR_3_PENALTY=1.966036
PRIMER_LEFT_3_PENALTY=1.223158
PRIMER_RIGHT_3_PENALTY=0.742877
PRIMER_LEFT_3_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC
PRIMER_RIGHT_3_SEQUENCE=AGAGAGCAAAGCACACAGGC
PRIMER_LEFT_3=37,21
PRIMER_RIGHT_3=187,20
PRIMER_LEFT_3_TM=60.223
PRIMER_RIGHT_3_TM=60.743
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=9.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=151
PRIMER_PAIR_3_PRODUCT_TM=74.7
PRIMER_PAIR_4_PENALTY=2.224300
PRIMER_LEFT_4_PENALTY=1.481423
PRIMER_RIGHT_4_PENALTY=0.742877
PRIMER_LEFT_4_SEQUENCE=TCTTTCTTTTTCCCATCTCCC
PRIMER_RIGHT_4_SEQUENCE=GAGAGCAAAGCACACAGGCT
PRIMER_LEFT_4=44,21
PRIMER_RIGHT_4=186,20
PRIMER_LEFT_4_TM=59.519
PRIMER_RIGHT_4_TM=60.743
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=143
PRIMER_PAIR_4_PRODUCT_TM=74.3
=
SEQUENCE_ID=39.RHAP34FH3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCTGTATGGAATAAAGTTTAAACATTTTTAAGTGTAACATAATAGTAAGCATACGACTGAAATGAATGTGATAGAGAAGGAAGTGGTTTGCCCAGCAAGGAATGTCATGACATCATAAAGTTATTCTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGATTATATACATGCATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGCTTCTTTTATTTCACATACAAAGGCCGTACTCTTGGAACTGTGAGTCAAAATAAGGACTTTGNCCNTG
SEQUENCE_INCLUDED_REGION=18,286
SEQUENCE_TARGET=148,32
SEQUENCE_TARGET=202,28
P3_COMMENT=CL is CCCAGCAAGGAATGTCATG
P3_COMMENT=CR is TTCCAAGAGTACGGCCTTTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.296841
PRIMER_LEFT_0_PENALTY=1.055809
PRIMER_RIGHT_0_PENALTY=0.241032
PRIMER_LEFT_0_SEQUENCE=CCCAGCAAGGAATGTCATG
PRIMER_RIGHT_0_SEQUENCE=TTCCAAGAGTACGGCCTTTG
PRIMER_LEFT_0=108,19
PRIMER_RIGHT_0=273,20
PRIMER_LEFT_0_TM=60.056
PRIMER_RIGHT_0_TM=60.241
PRIMER_LEFT_0_GC_PERCENT=52.632
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=166
PRIMER_PAIR_0_PRODUCT_TM=65.2
PRIMER_PAIR_1_PENALTY=1.321524
PRIMER_LEFT_1_PENALTY=1.055809
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=CCCAGCAAGGAATGTCATG
PRIMER_RIGHT_1_SEQUENCE=TCCAAGAGTACGGCCTTTGT
PRIMER_LEFT_1=108,19
PRIMER_RIGHT_1=272,20
PRIMER_LEFT_1_TM=60.056
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=65.2
PRIMER_PAIR_2_PENALTY=1.703177
PRIMER_LEFT_2_PENALTY=1.055809
PRIMER_RIGHT_2_PENALTY=0.647368
PRIMER_LEFT_2_SEQUENCE=CCCAGCAAGGAATGTCATG
PRIMER_RIGHT_2_SEQUENCE=CAGTTCCAAGAGTACGGCCT
PRIMER_LEFT_2=108,19
PRIMER_RIGHT_2=276,20
PRIMER_LEFT_2_TM=60.056
PRIMER_RIGHT_2_TM=59.353
PRIMER_LEFT_2_GC_PERCENT=52.632
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=169
PRIMER_PAIR_2_PRODUCT_TM=65.6
PRIMER_PAIR_3_PENALTY=1.844043
PRIMER_LEFT_3_PENALTY=1.055809
PRIMER_RIGHT_3_PENALTY=0.788233
PRIMER_LEFT_3_SEQUENCE=CCCAGCAAGGAATGTCATG
PRIMER_RIGHT_3_SEQUENCE=ACAGTTCCAAGAGTACGGCC
PRIMER_LEFT_3=108,19
PRIMER_RIGHT_3=277,20
PRIMER_LEFT_3_TM=60.056
PRIMER_RIGHT_3_TM=59.212
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=170
PRIMER_PAIR_3_PRODUCT_TM=65.5
PRIMER_PAIR_4_PENALTY=2.237217
PRIMER_LEFT_4_PENALTY=1.055809
PRIMER_RIGHT_4_PENALTY=1.181407
PRIMER_LEFT_4_SEQUENCE=CCCAGCAAGGAATGTCATG
PRIMER_RIGHT_4_SEQUENCE=ACAGTTCCAAGAGTACGGCCT
PRIMER_LEFT_4=108,19
PRIMER_RIGHT_4=277,21
PRIMER_LEFT_4_TM=60.056
PRIMER_RIGHT_4_TM=60.181
PRIMER_LEFT_4_GC_PERCENT=52.632
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=170
PRIMER_PAIR_4_PRODUCT_TM=65.5
=
SEQUENCE_ID=40.RHAP33FD4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCCGAAAAAAAGAAAAGAAAAAAAATTTAGTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACAGATATCAGATTTTAATATGTGCTCTTCTTACTCACACTCTTCCACCTACCTTTCCTCCCTGGCGAACTTCTGCCCGGCTACTCTCTCCTCATTCCCACCCCAGATAATAGGTCTTTCCTTGCCTCTGTGCCTCTATGGCAATATGAATCTAGTTCTATTCTAACATGCTTTTGTTGATAATCATGCTTTGGGGGTATCTTCCATCCTGTCCACAAGCCTTTTCCCATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=23,305
SEQUENCE_TARGET=61,32
P3_COMMENT=CL is TCCGAAAAAAAGAAAAGAAAAAAA
P3_COMMENT=CR is AGGGAGGAAAGGTAGGTGGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.394016
PRIMER_LEFT_0_PENALTY=4.322131
PRIMER_RIGHT_0_PENALTY=0.071885
PRIMER_LEFT_0_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
PRIMER_RIGHT_0_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
PRIMER_LEFT_0=24,24
PRIMER_RIGHT_0=158,20
PRIMER_LEFT_0_TM=59.678
PRIMER_RIGHT_0_TM=59.928
PRIMER_LEFT_0_GC_PERCENT=20.833
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=135
PRIMER_PAIR_0_PRODUCT_TM=66.4
PRIMER_PAIR_1_PENALTY=4.508176
PRIMER_LEFT_1_PENALTY=4.436291
PRIMER_RIGHT_1_PENALTY=0.071885
PRIMER_LEFT_1_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAA
PRIMER_RIGHT_1_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
PRIMER_LEFT_1=24,23
PRIMER_RIGHT_1=158,20
PRIMER_LEFT_1_TM=58.564
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=21.739
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=135
PRIMER_PAIR_1_PRODUCT_TM=66.4
PRIMER_PAIR_2_PENALTY=4.620407
PRIMER_LEFT_2_PENALTY=4.322131
PRIMER_RIGHT_2_PENALTY=0.298276
PRIMER_LEFT_2_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
PRIMER_RIGHT_2_SEQUENCE=GGGAGGAAAGGTAGGTGGAA
PRIMER_LEFT_2=24,24
PRIMER_RIGHT_2=157,20
PRIMER_LEFT_2_TM=59.678
PRIMER_RIGHT_2_TM=60.298
PRIMER_LEFT_2_GC_PERCENT=20.833
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=134
PRIMER_PAIR_2_PRODUCT_TM=66.4
PRIMER_PAIR_3_PENALTY=4.693497
PRIMER_LEFT_3_PENALTY=4.621612
PRIMER_RIGHT_3_PENALTY=0.071885
PRIMER_LEFT_3_SEQUENCE=CTCCGAAAAAAAGAAAAGAAAAAA
PRIMER_RIGHT_3_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
PRIMER_LEFT_3=23,24
PRIMER_RIGHT_3=158,20
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=136
PRIMER_PAIR_3_PRODUCT_TM=66.6
PRIMER_PAIR_4_PENALTY=4.725092
PRIMER_LEFT_4_PENALTY=4.322131
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
PRIMER_RIGHT_4_SEQUENCE=AGAGTAGCCGGGCAGAAGTT
PRIMER_LEFT_4=24,24
PRIMER_RIGHT_4=182,20
PRIMER_LEFT_4_TM=59.678
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=20.833
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=159
PRIMER_PAIR_4_PRODUCT_TM=69.3
=
SEQUENCE_ID=40.RHAP33FH4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTCCCACCTTCCTCATGTTGCCTTGCTCCAGTGGACAGCCAATTATGACAGATGATGGTGGAGGGACAAGATGTGCCCATATCCCCTCTCCCTTCATATCTTCTACTTCAGCAAGGGAGAGGCTAGCAGAGGGGGTTGAGTGTGTGTTCAGTAAGAAGGGACAGAGTAGTGCTAATGTGGGTGGCTTTCCCCACTGTTCTGGGGAAGACTGAANNNNNNNNNNNNNNNNNNNNNNTACAGAACTCAAAGTCTTTAATTCTGTGATGCATGGTAGAATTCATAGTAATGATAGAAAAATGGTTATTTTATTTTCTGTTCTAGNANGTNGGAAGGCATCGTGAACNAGTTTGNAGGAAAAAAGANCATT
SEQUENCE_INCLUDED_REGION=15,367
SEQUENCE_TARGET=228,22
P3_COMMENT=CL is AAGGGAGAGGCTAGCAGAGG
P3_COMMENT=CR is TGAATTCTACCATGCATCACAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.458937
PRIMER_LEFT_0_PENALTY=0.110131
PRIMER_RIGHT_0_PENALTY=3.348806
PRIMER_LEFT_0_SEQUENCE=AAGGGAGAGGCTAGCAGAGG
PRIMER_RIGHT_0_SEQUENCE=TGAATTCTACCATGCATCACAG
PRIMER_LEFT_0=127,20
PRIMER_RIGHT_0=294,22
PRIMER_LEFT_0_TM=60.110
PRIMER_RIGHT_0_TM=58.651
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=168
PRIMER_PAIR_0_PRODUCT_TM=72.9
PRIMER_PAIR_1_PENALTY=3.488065
PRIMER_LEFT_1_PENALTY=0.139259
PRIMER_RIGHT_1_PENALTY=3.348806
PRIMER_LEFT_1_SEQUENCE=CTAGCAGAGGGGGTTGAGTG
PRIMER_RIGHT_1_SEQUENCE=TGAATTCTACCATGCATCACAG
PRIMER_LEFT_1=137,20
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=58.651
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=158
PRIMER_PAIR_1_PRODUCT_TM=72.2
PRIMER_PAIR_2_PENALTY=3.489891
PRIMER_LEFT_2_PENALTY=0.141085
PRIMER_RIGHT_2_PENALTY=3.348806
PRIMER_LEFT_2_SEQUENCE=AGGGGGTTGAGTGTGTGTTC
PRIMER_RIGHT_2_SEQUENCE=TGAATTCTACCATGCATCACAG
PRIMER_LEFT_2=144,20
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=58.651
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=151
PRIMER_PAIR_2_PRODUCT_TM=71.7
PRIMER_PAIR_3_PENALTY=3.489891
PRIMER_LEFT_3_PENALTY=0.141085
PRIMER_RIGHT_3_PENALTY=3.348806
PRIMER_LEFT_3_SEQUENCE=GAGGGGGTTGAGTGTGTGTT
PRIMER_RIGHT_3_SEQUENCE=TGAATTCTACCATGCATCACAG
PRIMER_LEFT_3=143,20
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=58.651
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=152
PRIMER_PAIR_3_PRODUCT_TM=71.9
PRIMER_PAIR_4_PENALTY=3.554405
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=3.444274
PRIMER_LEFT_4_SEQUENCE=AAGGGAGAGGCTAGCAGAGG
PRIMER_RIGHT_4_SEQUENCE=TGAATTCTACCATGCATCACA
PRIMER_LEFT_4=127,20
PRIMER_RIGHT_4=294,21
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=57.556
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=168
PRIMER_PAIR_4_PRODUCT_TM=72.9
=
SEQUENCE_ID=40.RHAP34FH4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCAACTGAGGCTTCTACTGTGACTGCATTAAAGCTCAGCTTGTTCCCTCTGCTTCCATGGGTTTTTATTCCCCCAAAGCAACATTTGCTATACCTACCTCTGGTCTTTAACTGGTATCAGCAGTCTCAGAACAGCAACCCGAGGTTAGGGTGTTACGCCTAGCTAGCCCCGCTGGCAGTAAAGAACCCACGACAGTGTGGGTGGGGCCAGCTATTTCCAAATGCCATGTGGTACAGAAGGTAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,283
SEQUENCE_TARGET=264,38
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=41.RHAP33FD5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTACAGTGTTTGGAGTTTGTCCTGACAGGTTTCTATTTTGCTCTGATGTAATATTTATTCATTATATCCCCTTTGCTGCCTTTATAACAGTTATGTGTATTCTGTGCCATCATCTGGTAGAAGTATGTCATTTGCTTTTTTAATTTTGATTTTACAGGGTATTATGATTAAAAGCCTGGAGTATCAGAAGACTCTGGACTTTTTAAACTGTACTGGATCAAATGCCTTTTGTNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=22,248
SEQUENCE_TARGET=254,15
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=41.RHAP33FH5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNTGACCAATCAGCATGTTTTATGTTAAGTTGAAAAGACACTGTGTTGTATGGATTACAGATTTGCTAGGTGGTTAAATATTCTTGGATTTTGGTAACACAGAGGCACATGTGCACGTGCACACATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGATGGGGGGTGTTAATGGGGGAGGGAGAAAGNAGGAAGCAAGAAGCNATCCCTAACTGTAGTGAGGCAGGTAATGNAGAAAAGTGTAACAGATACCGACCTTTAGACAAGACTGCAGATATTAGANAAGCGATGGCTCTGTAATATAAATTTGTTAAGACATAGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=14,343
SEQUENCE_TARGET=142,42
P3_COMMENT=CL is CTTGGATTTTGGTAACACAGAGG
P3_COMMENT=CR is CTTTCTCCCTCCCCCATTAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.336015
PRIMER_LEFT_0_PENALTY=3.085878
PRIMER_RIGHT_0_PENALTY=0.250137
PRIMER_LEFT_0_SEQUENCE=CTTGGATTTTGGTAACACAGAGG
PRIMER_RIGHT_0_SEQUENCE=CTTTCTCCCTCCCCCATTAA
PRIMER_LEFT_0=94,23
PRIMER_RIGHT_0=218,20
PRIMER_LEFT_0_TM=59.914
PRIMER_RIGHT_0_TM=60.250
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=125
PRIMER_PAIR_0_PRODUCT_TM=68.6
PRIMER_PAIR_1_PENALTY=3.493328
PRIMER_LEFT_1_PENALTY=3.369403
PRIMER_RIGHT_1_PENALTY=0.123924
PRIMER_LEFT_1_SEQUENCE=TGGATTACAGATTTGCTAGGTGG
PRIMER_RIGHT_1_SEQUENCE=TTTCTCCCTCCCCCATTAAC
PRIMER_LEFT_1=62,23
PRIMER_RIGHT_1=217,20
PRIMER_LEFT_1_TM=60.369
PRIMER_RIGHT_1_TM=60.124
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=156
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=3.502257
PRIMER_LEFT_2_PENALTY=1.375502
PRIMER_RIGHT_2_PENALTY=2.126755
PRIMER_LEFT_2_SEQUENCE=TTGGTAACACAGAGGCACATG
PRIMER_RIGHT_2_SEQUENCE=CCTGCCTCACTACAGTTAGGG
PRIMER_LEFT_2=102,21
PRIMER_RIGHT_2=257,21
PRIMER_LEFT_2_TM=59.624
PRIMER_RIGHT_2_TM=58.873
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=156
PRIMER_PAIR_2_PRODUCT_TM=71.0
PRIMER_PAIR_3_PENALTY=3.619540
PRIMER_LEFT_3_PENALTY=3.369403
PRIMER_RIGHT_3_PENALTY=0.250137
PRIMER_LEFT_3_SEQUENCE=TGGATTACAGATTTGCTAGGTGG
PRIMER_RIGHT_3_SEQUENCE=CTTTCTCCCTCCCCCATTAA
PRIMER_LEFT_3=62,23
PRIMER_RIGHT_3=218,20
PRIMER_LEFT_3_TM=60.369
PRIMER_RIGHT_3_TM=60.250
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=69.4
PRIMER_PAIR_4_PENALTY=3.676869
PRIMER_LEFT_4_PENALTY=1.375502
PRIMER_RIGHT_4_PENALTY=2.301367
PRIMER_LEFT_4_SEQUENCE=TTGGTAACACAGAGGCACATG
PRIMER_RIGHT_4_SEQUENCE=ACCTGCCTCACTACAGTTAGGG
PRIMER_LEFT_4=102,21
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=59.624
PRIMER_RIGHT_4_TM=59.699
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=157
PRIMER_PAIR_4_PRODUCT_TM=71.0
=
SEQUENCE_ID=41.RHAP34FD5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNATATATTCTATGTATCTATGCNNNNNNNNNNNNNNNNNNNNAATTTATTTATTATTTTACCTTTGGGAAATACTCTCTTTGCAAAGCTTATAATGACTCCATGATAATTAAAAGCAGCAGGGGTTCAAGAGGCAAGAATGCATCTAGGTCTTAGCAAAATGGCAAATGCTGAGACCTTCAAGACCGTCATTAAGGCTGTGGGAATGAACTCTGAAAACATGTAGTTCAAATATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,236
SEQUENCE_TARGET=40,20
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=41.RHAP34FH5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCAGGCATCTCTGAGGGAAAGCAGGAGAAGGAGTCTAATTCCTCTGCAAACTAATTAATGAGCCTTCCCAGAGTGCTGGCAGCTAACAGGCAAAACTGAGAACCACTAACAGCAAGTACACCAAAGCCAGTAAGTAGCAATGCTTTCCAGGTGTGTGCATGTATACCNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGAGCACCCATGCATGTCATAGTGTGCATGGAGGTCAGAGGACAGCCCTGCCTTCTGCTCTTTGCCTTTCACTCTGTTTCAGACAGAC
SEQUENCE_INCLUDED_REGION=18,282
SEQUENCE_TARGET=184,26
P3_COMMENT=CL is CAGCAAGTACACCAAAGCCA
P3_COMMENT=CR is GTCCTCTGACCTCCATGCAC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.784472
PRIMER_LEFT_0_PENALTY=0.095506
PRIMER_RIGHT_0_PENALTY=0.688966
PRIMER_LEFT_0_SEQUENCE=CAGCAAGTACACCAAAGCCA
PRIMER_RIGHT_0_SEQUENCE=GTCCTCTGACCTCCATGCAC
PRIMER_LEFT_0=126,20
PRIMER_RIGHT_0=254,20
PRIMER_LEFT_0_TM=59.904
PRIMER_RIGHT_0_TM=60.689
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=129
PRIMER_PAIR_0_PRODUCT_TM=71.5
PRIMER_PAIR_1_PENALTY=0.794904
PRIMER_LEFT_1_PENALTY=0.347100
PRIMER_RIGHT_1_PENALTY=0.447804
PRIMER_LEFT_1_SEQUENCE=CAGAGTGCTGGCAGCTAACA
PRIMER_RIGHT_1_SEQUENCE=CTATGACATGCATGGGTGCT
PRIMER_LEFT_1=85,20
PRIMER_RIGHT_1=233,20
PRIMER_LEFT_1_TM=60.347
PRIMER_RIGHT_1_TM=59.552
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=149
PRIMER_PAIR_1_PRODUCT_TM=72.1
PRIMER_PAIR_2_PENALTY=0.860552
PRIMER_LEFT_2_PENALTY=0.347100
PRIMER_RIGHT_2_PENALTY=0.513452
PRIMER_LEFT_2_SEQUENCE=CAGAGTGCTGGCAGCTAACA
PRIMER_RIGHT_2_SEQUENCE=TATGACATGCATGGGTGCTC
PRIMER_LEFT_2=85,20
PRIMER_RIGHT_2=232,20
PRIMER_LEFT_2_TM=60.347
PRIMER_RIGHT_2_TM=60.513
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=148
PRIMER_PAIR_2_PRODUCT_TM=71.9
PRIMER_PAIR_3_PENALTY=0.900981
PRIMER_LEFT_3_PENALTY=0.095506
PRIMER_RIGHT_3_PENALTY=0.805475
PRIMER_LEFT_3_SEQUENCE=CAGCAAGTACACCAAAGCCA
PRIMER_RIGHT_3_SEQUENCE=GAAAGGCAAAGAGCAGAAGG
PRIMER_LEFT_3=126,20
PRIMER_RIGHT_3=281,20
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=59.195
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=156
PRIMER_PAIR_3_PRODUCT_TM=73.4
PRIMER_PAIR_4_PENALTY=0.924142
PRIMER_LEFT_4_PENALTY=0.095506
PRIMER_RIGHT_4_PENALTY=0.828636
PRIMER_LEFT_4_SEQUENCE=CAGCAAGTACACCAAAGCCA
PRIMER_RIGHT_4_SEQUENCE=CTGTCCTCTGACCTCCATGC
PRIMER_LEFT_4=126,20
PRIMER_RIGHT_4=256,20
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.829
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=131
PRIMER_PAIR_4_PRODUCT_TM=71.6
=
SEQUENCE_ID=43.RHAP34FD7.seq
SEQUENCE_TEMPLATE=ANACCCCACCNCCAGCNCCNCACACCNCAACCAGAACNGGTCNNNCNNTNTNNGTGCNNNNNNNNNNNNNNNNATCNCNCNGTGTGTGCCCNGCTCNGTATCNGTCNGNGTGTCNNGCCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTGTCTCGTCGTGTGTGATTGATGTTGTGNGTCNTGTCGTGATGCTGNTCGTCTCTGATGTGNTNNGNNGNNTGNTGTTGNTAGNTNNTAGTGNNGNTANNGNTNCNNGCTCNTGNTNNNGNTCCNNGNTNNTCANNGANGNNGATNCTCGCTCNTNGNTGNTGATGNNGCACCNGNTGTNGCNGATA
SEQUENCE_INCLUDED_REGION=0,340
SEQUENCE_TARGET=59,14
SEQUENCE_TARGET=124,24
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=44.RHAP33FD8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNTGCCATATTTCCCNAGCCATTAACTCATCTGACATAGGCCTTCTTCACGTTGCATCTAGATAGGTCTTCCTGTCTTCCTCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGAGAGAGAAGGAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=11,179
SEQUENCE_TARGET=95,56
SEQUENCE_TARGET=174,16
P3_COMMENT=CL is GCCTTCTTCACGTTGCATCT
P3_COMMENT=CR is CCCCCTCCTTCTCTCTCTCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.426207
PRIMER_LEFT_0_PENALTY=0.405717
PRIMER_RIGHT_0_PENALTY=1.020490
PRIMER_LEFT_0_SEQUENCE=GCCTTCTTCACGTTGCATCT
PRIMER_RIGHT_0_SEQUENCE=CCCCCTCCTTCTCTCTCTCT
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=172,20
PRIMER_LEFT_0_TM=60.406
PRIMER_RIGHT_0_TM=58.980
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=1.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=125
PRIMER_PAIR_0_PRODUCT_TM=66.6
PRIMER_PAIR_1_PENALTY=1.660714
PRIMER_LEFT_1_PENALTY=0.405717
PRIMER_RIGHT_1_PENALTY=1.254998
PRIMER_LEFT_1_SEQUENCE=GCCTTCTTCACGTTGCATCT
PRIMER_RIGHT_1_SEQUENCE=CCCCCCTCCTTCTCTCTCT
PRIMER_LEFT_1=48,20
PRIMER_RIGHT_1=173,19
PRIMER_LEFT_1_TM=60.406
PRIMER_RIGHT_1_TM=59.745
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=63.158
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=1.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=126
PRIMER_PAIR_1_PRODUCT_TM=66.9
PRIMER_PAIR_2_PENALTY=1.680443
PRIMER_LEFT_2_PENALTY=0.659953
PRIMER_RIGHT_2_PENALTY=1.020490
PRIMER_LEFT_2_SEQUENCE=ATAGGCCTTCTTCACGTTGC
PRIMER_RIGHT_2_SEQUENCE=CCCCCTCCTTCTCTCTCTCT
PRIMER_LEFT_2=44,20
PRIMER_RIGHT_2=172,20
PRIMER_LEFT_2_TM=59.340
PRIMER_RIGHT_2_TM=58.980
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=129
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=1.728423
PRIMER_LEFT_3_PENALTY=0.405717
PRIMER_RIGHT_3_PENALTY=1.322707
PRIMER_LEFT_3_SEQUENCE=GCCTTCTTCACGTTGCATCT
PRIMER_RIGHT_3_SEQUENCE=CCCCCTCCTTCTCTCTCTCTT
PRIMER_LEFT_3=48,20
PRIMER_RIGHT_3=172,21
PRIMER_LEFT_3_TM=60.406
PRIMER_RIGHT_3_TM=60.323
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=1.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=125
PRIMER_PAIR_3_PRODUCT_TM=66.6
PRIMER_PAIR_4_PENALTY=1.914951
PRIMER_LEFT_4_PENALTY=0.659953
PRIMER_RIGHT_4_PENALTY=1.254998
PRIMER_LEFT_4_SEQUENCE=ATAGGCCTTCTTCACGTTGC
PRIMER_RIGHT_4_SEQUENCE=CCCCCCTCCTTCTCTCTCT
PRIMER_LEFT_4=44,20
PRIMER_RIGHT_4=173,19
PRIMER_LEFT_4_TM=59.340
PRIMER_RIGHT_4_TM=59.745
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=130
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=44.RHAP34FD8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNACTCCAAATAGTGCCTACATCCCATATTATTCTATCTTTGCTATCAATTGATTTTTTTCACAAAGTTTCTCTTCCTCCCAAATTAGAATGATTTCTCTCCCTTGTTTGGTTGTATGTTCATTCTTAAGCTGTAGTTAACACTGTGCCTTAGAACATCTTATTACAAGATCCCATTTAGCATTTACATAACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGCCATGGAGCACTATTATTTGTTGGTAATTGNTTAAATGCAAAAAACAATGTCCCTTG
SEQUENCE_INCLUDED_REGION=11,284
SEQUENCE_TARGET=204,30
P3_COMMENT=CL is TTTCTCTCCCTTGTTTGGTTG
P3_COMMENT=CR is CAAATAATAGTGCTCCATGGCTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.246089
PRIMER_LEFT_0_PENALTY=1.799253
PRIMER_RIGHT_0_PENALTY=3.446835
PRIMER_LEFT_0_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG
PRIMER_RIGHT_0_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
PRIMER_LEFT_0=102,21
PRIMER_RIGHT_0=257,23
PRIMER_LEFT_0_TM=59.201
PRIMER_RIGHT_0_TM=59.553
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=156
PRIMER_PAIR_0_PRODUCT_TM=67.6
PRIMER_PAIR_1_PENALTY=5.290334
PRIMER_LEFT_1_PENALTY=1.843499
PRIMER_RIGHT_1_PENALTY=3.446835
PRIMER_LEFT_1_SEQUENCE=TGATTTCTCTCCCTTGTTTGG
PRIMER_RIGHT_1_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
PRIMER_LEFT_1=99,21
PRIMER_RIGHT_1=257,23
PRIMER_LEFT_1_TM=59.157
PRIMER_RIGHT_1_TM=59.553
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=159
PRIMER_PAIR_1_PRODUCT_TM=67.7
PRIMER_PAIR_2_PENALTY=5.366867
PRIMER_LEFT_2_PENALTY=1.799253
PRIMER_RIGHT_2_PENALTY=3.567613
PRIMER_LEFT_2_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG
PRIMER_RIGHT_2_SEQUENCE=AACAAATAATAGTGCTCCATGGC
PRIMER_LEFT_2=102,21
PRIMER_RIGHT_2=259,23
PRIMER_LEFT_2_TM=59.201
PRIMER_RIGHT_2_TM=59.432
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=67.5
PRIMER_PAIR_3_PENALTY=5.411112
PRIMER_LEFT_3_PENALTY=1.843499
PRIMER_RIGHT_3_PENALTY=3.567613
PRIMER_LEFT_3_SEQUENCE=TGATTTCTCTCCCTTGTTTGG
PRIMER_RIGHT_3_SEQUENCE=AACAAATAATAGTGCTCCATGGC
PRIMER_LEFT_3=99,21
PRIMER_RIGHT_3=259,23
PRIMER_LEFT_3_TM=59.157
PRIMER_RIGHT_3_TM=59.432
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=161
PRIMER_PAIR_3_PRODUCT_TM=67.6
PRIMER_PAIR_4_PENALTY=5.458989
PRIMER_LEFT_4_PENALTY=2.012153
PRIMER_RIGHT_4_PENALTY=3.446835
PRIMER_LEFT_4_SEQUENCE=TTTCTCTCCCTTGTTTGGTTGT
PRIMER_RIGHT_4_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
PRIMER_LEFT_4=102,22
PRIMER_RIGHT_4=257,23
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.553
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=156
PRIMER_PAIR_4_PRODUCT_TM=67.6
=
SEQUENCE_ID=45.RHAP33FD9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNCTCGTTATGAAAAATTACATCTAATTGGAAGGTAGTTGAGTGAGTGGAGTGAAGTTGAGTTCAGTTCATTTCAGTGAGTCAGTGCAGTGGAATTGTGTTCATGCAATTCAGTGTAGGTCAGCTGGAGGCCCTTGTCCAGGCCACATGTAATACAGAAACAAAAATAGGGAATAGTACCAAGGAACTCTCACTAGAATAAAAGCAAGGAGTGTCCGCAGCACTGTGTTTATTCCTAATGNNNNNNNNNNNNNNNNGCCTCCCAAGGAGAGTCTGCCATGACCCCAGGTAAGAACTCACGCTGCATATGCCAATCCTTTGCTAGAGCATGTGAGCACCACGCCCACTCGACACAAACTTT
SEQUENCE_INCLUDED_REGION=11,358
SEQUENCE_TARGET=249,16
P3_COMMENT=CL is TAAAAGCAAGGAGTGTCCGC
P3_COMMENT=CR is AAAGTTTGTGTCGAGTGGGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.542573
PRIMER_LEFT_0_PENALTY=0.386286
PRIMER_RIGHT_0_PENALTY=0.156287
PRIMER_LEFT_0_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
PRIMER_RIGHT_0_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
PRIMER_LEFT_0=207,20
PRIMER_RIGHT_0=368,20
PRIMER_LEFT_0_TM=60.386
PRIMER_RIGHT_0_TM=60.156
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=11.4000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=162
PRIMER_PAIR_0_PRODUCT_TM=74.9
PRIMER_PAIR_1_PENALTY=0.554311
PRIMER_LEFT_1_PENALTY=0.386286
PRIMER_RIGHT_1_PENALTY=0.168025
PRIMER_LEFT_1_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
PRIMER_RIGHT_1_SEQUENCE=GGTGCTCACATGCTCTAGCA
PRIMER_LEFT_1=207,20
PRIMER_RIGHT_1=346,20
PRIMER_LEFT_1_TM=60.386
PRIMER_RIGHT_1_TM=60.168
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=140
PRIMER_PAIR_1_PRODUCT_TM=74.1
PRIMER_PAIR_2_PENALTY=0.554311
PRIMER_LEFT_2_PENALTY=0.386286
PRIMER_RIGHT_2_PENALTY=0.168025
PRIMER_LEFT_2_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
PRIMER_RIGHT_2_SEQUENCE=TGGTGCTCACATGCTCTAGC
PRIMER_LEFT_2=207,20
PRIMER_RIGHT_2=347,20
PRIMER_LEFT_2_TM=60.386
PRIMER_RIGHT_2_TM=60.168
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=141
PRIMER_PAIR_2_PRODUCT_TM=74.0
PRIMER_PAIR_3_PENALTY=0.809785
PRIMER_LEFT_3_PENALTY=0.653497
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=CGCAGCACTGTGTTTATTCC
PRIMER_RIGHT_3_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
PRIMER_LEFT_3=224,20
PRIMER_RIGHT_3=368,20
PRIMER_LEFT_3_TM=59.347
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=145
PRIMER_PAIR_3_PRODUCT_TM=74.7
PRIMER_PAIR_4_PENALTY=0.809785
PRIMER_LEFT_4_PENALTY=0.653497
PRIMER_RIGHT_4_PENALTY=0.156287
PRIMER_LEFT_4_SEQUENCE=CCGCAGCACTGTGTTTATTC
PRIMER_RIGHT_4_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
PRIMER_LEFT_4=223,20
PRIMER_RIGHT_4=368,20
PRIMER_LEFT_4_TM=59.347
PRIMER_RIGHT_4_TM=60.156
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=146
PRIMER_PAIR_4_PRODUCT_TM=74.9
=
SEQUENCE_ID=46.RHAP33FH10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNACCTGAGAAATTACCTTGGGTTTGGTAGAACCAGGCAGGAATGAATGATGAAGAAATAAGTGAATAGGTCTTCAGCAGAGGGATTTCTGACCTTCTAGCTATTCATCACTTCTCATAAGTATCTAAGCTTTATAAGTGAGTTTTATCCAGTATGTCATGAGCTCGTGTTTATTTATATGTATTTGGATAAGCGTAAATACAAATGAACACTAATAAAAATACTTGTTAAAAATAGAAATCAAACAAATACACAAAGCAAAGAGATATNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNCGTTTTTATATGANNNCAACCNACCNNGGNAGAAANAANGTNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=26,355
SEQUENCE_TARGET=293,27
SEQUENCE_TARGET=321,19
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=46.RHAP34FD10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTGCTGGATCTGTTTAGTGTTGTTCACGTGCATATGTTTCTAGGCCTAACCACTTACTAGGTATTGGATAATGGATTAGGGCTCAAACCTGGGATGACTGATACTCCTTTTCTCAATAGTTGGATAATGGATTAGGAGCTCAAACCTGGAGAAGACTAATACTCCTTCTCTCAGTAGTTGGATAATAGATTAGGGGCTCAAACCTGGAGAAGACTAATACTCC
SEQUENCE_INCLUDED_REGION=0,281
SEQUENCE_TARGET=0,56
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=47.RHAP33FD11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNCTGTTTTNGACCTCTTCCTCCTGGAAGGTCAAACTCTAAAGAGGGAACTATAGGGAAGAGTTAGTGGGGCCTTTAAATTGAGCAGCAACCACAAACTCAGTGGGTAGGAAAAAGAGAACAACAAGCTAGCAGGGTCTTTGTTCACCTAGATTCTTTCATATTCTGGGTTTTAAAGAAGTTACTATACATTAACTGTCCTGAAGATGTCCTTAGATAAGTTCCTCGCCCACTTGCCCCACTTCTGTATCCATCCACATTTAAATTCTCTAAAAGGCCATGAATCTTACTTCATTCCATGACTTTTCCCCTAGGGAAATNNNNNNNNNNNNNNNNTGTATGTATGNNNNNNNNNNNNNNNT
SEQUENCE_INCLUDED_REGION=11,359
SEQUENCE_TARGET=328,16
SEQUENCE_TARGET=354,15
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=48.RHAP33FD12.seq
SEQUENCE_TEMPLATE=CNTTTTANGGGGTTGGTGGTTCATCTGAAAGTGTTGAGCACCTTTTCTACTCCTGCTTATATATTTTTATCAAAAGAAGAAGAAGAAGGAGAAGAAAAATGAAATAGCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGANTGATGTGTTTGTACGGNNAGNATGAGNTTTCATNGTTCTCTTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGAAGGATGCTNNTCCTATACANTGAGAGAATATCCTTTATTACNCTGNCNTTAGTTTGTTACATGGAAGAATTTTTGCCTGCNGGTTTAGCCCTCAGGCAAGNCGNG
SEQUENCE_INCLUDED_REGION=0,361
SEQUENCE_TARGET=112,58
SEQUENCE_TARGET=222,28
P3_COMMENT=CL is GGGTTGGTGGTTCATCTGAA
P3_COMMENT=CR is GCAGGCAAAAATTCTTCCAT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.596137
PRIMER_LEFT_0_PENALTY=0.758218
PRIMER_RIGHT_0_PENALTY=0.837920
PRIMER_LEFT_0_SEQUENCE=GGGTTGGTGGTTCATCTGAA
PRIMER_RIGHT_0_SEQUENCE=GCAGGCAAAAATTCTTCCAT
PRIMER_LEFT_0=9,20
PRIMER_RIGHT_0=335,20
PRIMER_LEFT_0_TM=60.758
PRIMER_RIGHT_0_TM=59.162
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=327
PRIMER_PAIR_0_PRODUCT_TM=68.5
PRIMER_PAIR_1_PENALTY=1.936516
PRIMER_LEFT_1_PENALTY=0.758218
PRIMER_RIGHT_1_PENALTY=1.178298
PRIMER_LEFT_1_SEQUENCE=GGGTTGGTGGTTCATCTGAA
PRIMER_RIGHT_1_SEQUENCE=CTTGCCTGAGGGCTAAACC
PRIMER_LEFT_1=9,20
PRIMER_RIGHT_1=355,19
PRIMER_LEFT_1_TM=60.758
PRIMER_RIGHT_1_TM=59.822
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=347
PRIMER_PAIR_1_PRODUCT_TM=69.3
PRIMER_PAIR_2_PENALTY=2.398813
PRIMER_LEFT_2_PENALTY=1.560893
PRIMER_RIGHT_2_PENALTY=0.837920
PRIMER_LEFT_2_SEQUENCE=GGTTGGTGGTTCATCTGAAAG
PRIMER_RIGHT_2_SEQUENCE=GCAGGCAAAAATTCTTCCAT
PRIMER_LEFT_2=10,21
PRIMER_RIGHT_2=335,20
PRIMER_LEFT_2_TM=59.439
PRIMER_RIGHT_2_TM=59.162
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=326
PRIMER_PAIR_2_PRODUCT_TM=68.4
PRIMER_PAIR_3_PENALTY=2.450989
PRIMER_LEFT_3_PENALTY=1.613069
PRIMER_RIGHT_3_PENALTY=0.837920
PRIMER_LEFT_3_SEQUENCE=GGTTGGTGGTTCATCTGAAA
PRIMER_RIGHT_3_SEQUENCE=GCAGGCAAAAATTCTTCCAT
PRIMER_LEFT_3=10,20
PRIMER_RIGHT_3=335,20
PRIMER_LEFT_3_TM=58.387
PRIMER_RIGHT_3_TM=59.162
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=326
PRIMER_PAIR_3_PRODUCT_TM=68.4
PRIMER_PAIR_4_PENALTY=2.535567
PRIMER_LEFT_4_PENALTY=1.697647
PRIMER_RIGHT_4_PENALTY=0.837920
PRIMER_LEFT_4_SEQUENCE=GGGTTGGTGGTTCATCTGA
PRIMER_RIGHT_4_SEQUENCE=GCAGGCAAAAATTCTTCCAT
PRIMER_LEFT_4=9,19
PRIMER_RIGHT_4=335,20
PRIMER_LEFT_4_TM=59.302
PRIMER_RIGHT_4_TM=59.162
PRIMER_LEFT_4_GC_PERCENT=52.632
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=327
PRIMER_PAIR_4_PRODUCT_TM=68.5
=
SEQUENCE_ID=48.RHAP34FD12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNACCTAGGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACAACTGTTAACGTAGGAAGCTTCGGTGATCTTCCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=10,120
SEQUENCE_TARGET=21,33
SEQUENCE_TARGET=57,32
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=48.RHAP34FH12.seq
SEQUENCE_TEMPLATE=CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTACAANAAGTCAGGGAGNGGGAGGAGTTCTGNACAGATGGCTTACTGAACCACAATGTGAAAAAGCATGACTTAATGGTGNTCATTTGAGTTTATCCCTTNTCAATACCAACGANAGGGAAGAANTTNAATTGACCAGACACACCGACNNTGAGGGGTCNNAATTGNAACACCNTAGAGAGAGACAAAGAAATGATACTNGNACAAGNTNTGNAAGAGAGNCACTATGTATGNAAGAAGTGNGAGAGNANACATTTCTCCNNGNCACGGTAATAAGCTNGAGAGAGNTNTANTCAACNAAGGAATACATNGGACATAAAAGNGNAAGAAAAGAATCNCATNGTCAACATTGNANTACACAATACACTGNAAGNAGCAAAGNTGA
SEQUENCE_INCLUDED_REGION=0,420
SEQUENCE_TARGET=1,34
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=03.RHAP61FA3.seq
SEQUENCE_TEMPLATE=GTCATTGTATTNATCACTACANCAATAAAAANGAAANNGCGACAACTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTNTGTATGTCNNTTTANNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNTNGNGTGTGNCCCCTGTATATATGTGCTTNNNNGTGTGANNGCNCGNNTATNNNTNTNNGATNCCNGNTGGNNNNNNNNNNNNNNNNGTCGTNNANNNANNNNNTGNNANNTNAAGGGNNTCNACCCCNANANCANNTCTNTAGGCNCCANNGAANNTNGAAATNNNNNANNNANNGTCATTANCCNGTNNNCTGNCTCCAAANNNNTANCCGNNCAANANNNCCCANAATCANNNCAAGNCNGAANTANNNANCCGCAAANNNNCNGGNNNCNNAANNNTGTCNNNNNNANNCACNNTTANNNCGCNCCCNCNNNTCACCNGCCAGNNTTGCANCNNNGNANAT
SEQUENCE_INCLUDED_REGION=0,485
SEQUENCE_TARGET=52,30
SEQUENCE_TARGET=102,16
SEQUENCE_TARGET=119,20
SEQUENCE_TARGET=213,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=04.RHAP61FB1.seq
SEQUENCE_TEMPLATE=AGACCCCCTTGTTGATGTGTTTGTGCACAGATCNGTATCTGTGAACACTGACAGAAGGGAGGAAGGAACTGAGTGTCTTGAAAGATATGAGGGAATATGGCTATCTCTGTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNTNNNNTTTTATATACATCTGCATCTTTNCATAGACACCCCATGAAAANGNACCTTGCAAATTCNTTATGGGNNANGTNTGNGTTTGTCAAAATNGGANTNNTCTTGNNAGCTAACTTCCGGNTNNNTAGAATGTATTATCCNCTNTATGTTNGACACTCTNTTGTTGNTGTGTTTCNTAAATAGGGGTANGCGCTCTGNNTTCTGTANNCNTNGGTCNTAGCTGTTTCCTGTTGGNAAATTTNTTNTGCGCNCNTATATTCCNCNNNACATTGTGNGGCGCANNCNTANTGTTTAAACNCNNGGGTCCCNNGTGAGGGCGCGTTTCGCNCNT
SEQUENCE_INCLUDED_REGION=0,538
SEQUENCE_TARGET=113,34
SEQUENCE_TARGET=148,54
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=04.RHAP61FB7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNTGTAGTGTAAGTACCANTTATAACCCTAAGCCAGNTTTGAGAGGCAATAAGTCAACACTTGGATGAGAAAGTGGNACCAGGCCTTTGGTTTGGTCAACAACCTTTTGAGGCAACCATATTATCACCCNTTATTTCACAGATGAAGAAGTTGCACAAAGAAAGTAAAGCAGTATCATATATACACACACATATATGTATACCTACCCATCATATATACATACATATGTTACATATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTAAGCTCCNNAGCCNGGCCTGCANNTGGGGGGATCCNNTGNCAGTGCACCGCACANTGTATCACTGCGNGAAGATCCGNCAGGGNNTCTAGTTTCNNCAACNGCNTNNCNGAGGGATGCNGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,401
SEQUENCE_TARGET=257,36
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=06.RHAP61FB3.seq
SEQUENCE_TEMPLATE=TCAAGATGGTGCAGAGGTAAAGGCAGTTGCTGCCAAGATTGAANACCTGACTTCAATCACTNNAACCTACATGTTGGAGGGAANAACGNACTCCAATTTTTCCTCTGACCTTCAACAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNTGCTTAAATTCAAACANTGANATTGTNATTGGCCAAAATGNTTNATTTCCGCTCACANTTTTANAGCATTCNGGGGTCGGNGGGCNTCCTCCNTNGGNATTTGNNCCNGGGNNANCNACNTTGTCGANAATAAGAACCATGNNCGCGCCAANGANGNNNANNGAGGGTTNCNNGCTCGNNTTNGTANNNNANGGTCANANNNGTTTCNTGGGNNNANNTGGTNTCNGGNNNNNAAATCNNNNCAANNTTCNNGGCCNTGGAGGNATTAAGGNGNNNGCCTTGGGGGGGNNANNGNGTNAGT
SEQUENCE_INCLUDED_REGION=0,528
SEQUENCE_TARGET=124,55
SEQUENCE_TARGET=181,40
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=06.RHAP61FB9.seq
SEQUENCE_TEMPLATE=CNTCCNCCNNCCNCCNCTCNCGCCNNNNNNNNNNNNNNNNNNCNNNNNCNNNNNNNNNNNCNNNCNGANTNTGGTGNAATNTGNNGNTCNCNTNGGGGATCNNNAANCNCCCNNANNNNNNNNNNGNNGNNGANNNGTNCNANNTTNNNNNTTGGNGNTNAANNNNNAAATNNNTGNTNNGNAGNANGNGGNNTAAGNNNNCNNTANANGANNNNCNNNANNNNGNNNNNAGTANNNNATNGGNNNNGTTTNGGANGNNGNNATNGNNNAGANNAANNGNGNTGNGACTAGGGNGATNNGGANAAGANGGNNANNGNAAGNGGNGTNNNGGNNTNNTGGNANNTGTNNTGNNNGANNGGNNGNGGNNTAGGANNGNNTGTNNNTNTGGTNNANAAGTNNGNNNTGNNNNTGNNNNNNGGGTTTNNGNANGNNNNNNNNNTTAANNGGNNNNNNANANNTANNTGNNAAAAGGNNNNNNGGGANNNGNNNTAAGNANNGGGNGTNNGGNNGNGGNNNNNTGTGNNNGNNGNTTGGNGNNTNGNAGNNNNGNNNNNNNANGNNNTGNNNAAANT
SEQUENCE_INCLUDED_REGION=0,572
SEQUENCE_TARGET=24,16
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=07.RHAP61FC7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNTGGGCGTGGTCCCGAGACACTCCCCGCGGGGGCGNCCGGGGCGGGGCGCGGCACCAGGTGGAGGGCGCTGACCCAAGCAACGTCAGTCTGGAAACTGCGTTCCCTAGGCATCTTATCCCCCTAGACCTAGCAGCAGAAGTACCCCAATTCAGTCTCTCAATTCAGTGCTCTTGGAGGAGAGTGTACCCACTCAAGGANTNNCNCGGGCTGGCTGAGCGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=11,267
SEQUENCE_TARGET=233,44
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=11.RHAP61FD8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTCAGAATTGTTGGGAAGACAGAGTGAAACCCATGGCCTGAGCAGTATCTAGTCTCGGTAANTAGCAAGAAGANAAGGCTAGAAAGGCTGAGGTCTAGATAGGGCTATCCTGGGCTAGACTGAGTGGGTGATCATGACCAACACCAAGGGACTCAGGTGAAGCCCTCTACAAAGGTCCAGACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGNGNGNNNNNNNNNNNNNNNGTCGTGNTGNGGTGTNNGNGTNNGNNNNNNNGGGTGTGNNGTGNNNNNNNGNNTNGGGGNNGNNNNNNNNGGNNNGNNGANNNAGNGGGNGGAGGNCTNTAGNGNANGNNGNNNNNNGGNNANAGNNNNNNNNNGGGTNNNNGNNGNNNANNNNGNNGGNNNANNGTGGGGTNNNGGGGNNAGNNGNNGGNNTNGNGNGAAGNGNNGGGGNNNTGNGNNANNGGGNTNGANNNGNGNGNNNGNGGNA
SEQUENCE_INCLUDED_REGION=19,483
SEQUENCE_TARGET=203,28
SEQUENCE_TARGET=240,15
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=12.RHAP61FD9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTGTTTTGGAAATTNGAGGAACAAAATATAAAANNCATTAAAAAATTCCCATTTCTGAAAATTATCACTGGAGTTANGGACAATTATGAGCCACACATTGTGGCTCATGATAAGAATNNNCAGAGGGAAANTANGATCTNNNTGNGAAAGAGGGACTNTGNAGNGNCNCNCNNANNGNCNNNAANNNNNCGNTNNGNNNCCNNCTCNNTCACTGNNNNNNNNNNNNNNNNCTNNTCNNTTTNTAGAATCGNGNNGNTGGTCTGAAAACNTNNNNGNNCACNNNNTCTTGGTTANCNTCANTTNTAAAATGNNTGANGNTGNTAGTAGGGNNGCNGCGTGNNNNCCNCCNTCCCAGGNNANCCNGNGCCCCATGNNNCNTCAAANCAANTNNNTGGANCCNCAANGTGNNANGGCCCNNCNATCCNNGNNNNTCNTGNNNNNGNGNTCATTGCAAANTNAGNGNNNNNNNNCNNNANTG
SEQUENCE_INCLUDED_REGION=21,478
SEQUENCE_TARGET=236,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=13.RHAP61FE7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTTGAACATTTACTACATTTCCTGGGCTCACCANTTTGTNAATTTGCCTTTTTCTGTTCCATATGCANTTTTACCATCATCAGGTATAATGAATGGGTCTTAGCAGATTCCACTTGGGTGNTCTANTGAATTCTNNNNNNNNNNNNNNNNNNNNNNNNNGNGNNNCCNCCCNCNNNNNCNNTGNNNNNNNNNNNTGNNNNNGNCNCNNNNNCGGNNNGNGGGNNGCTGNGGAANNNNGGNNGNGNNGGGGNGNNNNGGGGNNNGNNNNGGGTGGGGGNGNNGCAGTGNNNNGGGGNNGNGGNGNGNNTGNGGNGTGGNNGNNNGGNNGTGNNNGGGNGGNGGGGGTTGGGNGNNGNNGTNNNAANNGGGGGNNNNGTNGGGGGNNGNNNNGNNNNNNNNNGGGANNNGGGGGNNTTNNNNGGGNGAGNNNGANNNGNNNNNGGGNNNANNNNNGAGGGNNGGNANNNGTGNGGGNNAGGGNNNGTTNNGGNNTTAAA
SEQUENCE_INCLUDED_REGION=21,497
SEQUENCE_TARGET=155,24
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=14.RHAP61FE8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTAGGACTCTGAAGTATATTCAGGTACTTGTGATGCTGAGGGAGCCTGAAGGCAGCACCGAGTTTTGGTGTGCTGATAGAAACCAAAGATAGTTATTTGTCCCTTCTGCCTTTTAGGATCTGGGGAAACGGAGTTTCTTTTGGACCTGACAGCAGTTTGCATGNGNGTCNGNGATGTATTTTGATCACGTTCACCGTTATCTTGTCCCCCTCCCACCCCTACAGACCTTCTACCTCTGTGTGTGCATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCANTGGTGTTACAGGAAAATGAGAGAGGTTCGTTACACTGGGGCACCTTGCCAGTGGCGTACATCACTGACAAAGTTTCTNTCCCTCCCAGCAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,401
SEQUENCE_TARGET=268,50
P3_COMMENT=CL is ACCGTTATCTTGTCCCCCTC
P3_COMMENT=CR is CCCCAGTGTAACGAACCTCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.128597
PRIMER_LEFT_0_PENALTY=0.190281
PRIMER_RIGHT_0_PENALTY=0.938316
PRIMER_LEFT_0_SEQUENCE=ACCGTTATCTTGTCCCCCTC
PRIMER_RIGHT_0_SEQUENCE=CCCCAGTGTAACGAACCTCT
PRIMER_LEFT_0=210,20
PRIMER_RIGHT_0=362,20
PRIMER_LEFT_0_TM=60.190
PRIMER_RIGHT_0_TM=59.062
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=153
PRIMER_PAIR_0_PRODUCT_TM=69.9
PRIMER_PAIR_1_PENALTY=1.147975
PRIMER_LEFT_1_PENALTY=0.209659
PRIMER_RIGHT_1_PENALTY=0.938316
PRIMER_LEFT_1_SEQUENCE=TTCACCGTTATCTTGTCCCC
PRIMER_RIGHT_1_SEQUENCE=CCCCAGTGTAACGAACCTCT
PRIMER_LEFT_1=207,20
PRIMER_RIGHT_1=362,20
PRIMER_LEFT_1_TM=59.790
PRIMER_RIGHT_1_TM=59.062
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=156
PRIMER_PAIR_1_PRODUCT_TM=70.0
PRIMER_PAIR_2_PENALTY=1.208793
PRIMER_LEFT_2_PENALTY=0.190281
PRIMER_RIGHT_2_PENALTY=1.018512
PRIMER_LEFT_2_SEQUENCE=ACCGTTATCTTGTCCCCCTC
PRIMER_RIGHT_2_SEQUENCE=GCCCCAGTGTAACGAACCT
PRIMER_LEFT_2=210,20
PRIMER_RIGHT_2=363,19
PRIMER_LEFT_2_TM=60.190
PRIMER_RIGHT_2_TM=59.981
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=154
PRIMER_PAIR_2_PRODUCT_TM=70.1
PRIMER_PAIR_3_PENALTY=1.228171
PRIMER_LEFT_3_PENALTY=0.209659
PRIMER_RIGHT_3_PENALTY=1.018512
PRIMER_LEFT_3_SEQUENCE=TTCACCGTTATCTTGTCCCC
PRIMER_RIGHT_3_SEQUENCE=GCCCCAGTGTAACGAACCT
PRIMER_LEFT_3=207,20
PRIMER_RIGHT_3=363,19
PRIMER_LEFT_3_TM=59.790
PRIMER_RIGHT_3_TM=59.981
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=70.2
PRIMER_PAIR_4_PENALTY=1.300852
PRIMER_LEFT_4_PENALTY=0.362536
PRIMER_RIGHT_4_PENALTY=0.938316
PRIMER_LEFT_4_SEQUENCE=CCCACCCCTACAGACCTTCT
PRIMER_RIGHT_4_SEQUENCE=CCCCAGTGTAACGAACCTCT
PRIMER_LEFT_4=229,20
PRIMER_RIGHT_4=362,20
PRIMER_LEFT_4_TM=60.363
PRIMER_RIGHT_4_TM=59.062
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=134
PRIMER_PAIR_4_PRODUCT_TM=68.3
=
SEQUENCE_ID=16.RHAP61FF1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNTGTCATTTTCTTTTTAATCTCTGTTTTTCTGCCATTCTAGGTCAGCTGAAGATGAAGGTCTCCTCTACTGCTCTGGAGCCTNTNCACGGAGACCCTAATTTCATGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTCCNTTGAAATAATGGAAGATATTAAAGTATAAAAATAAGAGAGTTGTACCATCCATACCCATTCTGAAAATGAAGCCAAATGTAATCTTATCACTAAAGAGCATGTTATATNTGAACAGAGACATTTTATACACTATAAGATTAGATCTCAAANTNNTTATNTACCTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=11,327
SEQUENCE_TARGET=120,44
P3_COMMENT=CL is TCCTCTACTGCTCTGGAGCC
P3_COMMENT=CR is GGCTTCATTTTCAGAATGGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.792069
PRIMER_LEFT_0_PENALTY=0.296722
PRIMER_RIGHT_0_PENALTY=0.495347
PRIMER_LEFT_0_SEQUENCE=TCCTCTACTGCTCTGGAGCC
PRIMER_RIGHT_0_SEQUENCE=GGCTTCATTTTCAGAATGGG
PRIMER_LEFT_0=71,20
PRIMER_RIGHT_0=244,20
PRIMER_LEFT_0_TM=59.703
PRIMER_RIGHT_0_TM=59.505
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=68.0
PRIMER_PAIR_1_PENALTY=0.967399
PRIMER_LEFT_1_PENALTY=0.472052
PRIMER_RIGHT_1_PENALTY=0.495347
PRIMER_LEFT_1_SEQUENCE=CGGAGACCCTAATTTCATGC
PRIMER_RIGHT_1_SEQUENCE=GGCTTCATTTTCAGAATGGG
PRIMER_LEFT_1=97,20
PRIMER_RIGHT_1=244,20
PRIMER_LEFT_1_TM=59.528
PRIMER_RIGHT_1_TM=59.505
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=148
PRIMER_PAIR_1_PRODUCT_TM=65.8
PRIMER_PAIR_2_PENALTY=1.532033
PRIMER_LEFT_2_PENALTY=0.472052
PRIMER_RIGHT_2_PENALTY=1.059980
PRIMER_LEFT_2_SEQUENCE=CGGAGACCCTAATTTCATGC
PRIMER_RIGHT_2_SEQUENCE=TTGGCTTCATTTTCAGAATGG
PRIMER_LEFT_2=97,20
PRIMER_RIGHT_2=246,21
PRIMER_LEFT_2_TM=59.528
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_2_PRODUCT_TM=65.7
PRIMER_PAIR_3_PENALTY=1.601557
PRIMER_LEFT_3_PENALTY=0.296722
PRIMER_RIGHT_3_PENALTY=1.304835
PRIMER_LEFT_3_SEQUENCE=TCCTCTACTGCTCTGGAGCC
PRIMER_RIGHT_3_SEQUENCE=TGGCTTCATTTTCAGAATGG
PRIMER_LEFT_3=71,20
PRIMER_RIGHT_3=245,20
PRIMER_LEFT_3_TM=59.703
PRIMER_RIGHT_3_TM=58.695
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=175
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=1.609992
PRIMER_LEFT_4_PENALTY=0.296722
PRIMER_RIGHT_4_PENALTY=1.313269
PRIMER_LEFT_4_SEQUENCE=TCCTCTACTGCTCTGGAGCC
PRIMER_RIGHT_4_SEQUENCE=GGCTTCATTTTCAGAATGGGT
PRIMER_LEFT_4=71,20
PRIMER_RIGHT_4=244,21
PRIMER_LEFT_4_TM=59.703
PRIMER_RIGHT_4_TM=60.313
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=68.0
=
SEQUENCE_ID=18.RHAP61FF3.seq
SEQUENCE_TEMPLATE=GTAGCCGACACCATCCCCCGACACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAAACAAACAAACAAACAAATCAGCAAAACGTTTAGTCAATTCTGCTTTTCTTACTGGAAAGCCCAAAGCCTGTGTCTTAGCATGGCTGATTATCCTCTCTTCTGGGCAAGATTTCCTCAGTGCCAGTTTTATGTTTGCAAGGTCCCATCTATGCCAAGCATCATGTTAGCCCTAATATTTCTGATGCATTCCAAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,271
SEQUENCE_TARGET=34,34
P3_COMMENT=CL is TCCCCCGACACATACATACA
P3_COMMENT=CR is TAAGACACAGGCTTTGGGCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.476466
PRIMER_LEFT_0_PENALTY=0.351810
PRIMER_RIGHT_0_PENALTY=0.124655
PRIMER_LEFT_0_SEQUENCE=TCCCCCGACACATACATACA
PRIMER_RIGHT_0_SEQUENCE=TAAGACACAGGCTTTGGGCT
PRIMER_LEFT_0=13,20
PRIMER_RIGHT_0=151,20
PRIMER_LEFT_0_TM=59.648
PRIMER_RIGHT_0_TM=59.875
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=139
PRIMER_PAIR_0_PRODUCT_TM=67.4
PRIMER_PAIR_1_PENALTY=0.476466
PRIMER_LEFT_1_PENALTY=0.351810
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TCCCCCGACACATACATACA
PRIMER_RIGHT_1_SEQUENCE=CTAAGACACAGGCTTTGGGC
PRIMER_LEFT_1=13,20
PRIMER_RIGHT_1=152,20
PRIMER_LEFT_1_TM=59.648
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=140
PRIMER_PAIR_1_PRODUCT_TM=67.6
PRIMER_PAIR_2_PENALTY=0.476466
PRIMER_LEFT_2_PENALTY=0.351810
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TCCCCCGACACATACATACA
PRIMER_RIGHT_2_SEQUENCE=GCTAAGACACAGGCTTTGGG
PRIMER_LEFT_2=13,20
PRIMER_RIGHT_2=153,20
PRIMER_LEFT_2_TM=59.648
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=141
PRIMER_PAIR_2_PRODUCT_TM=67.9
PRIMER_PAIR_3_PENALTY=0.779333
PRIMER_LEFT_3_PENALTY=0.351810
PRIMER_RIGHT_3_PENALTY=0.427522
PRIMER_LEFT_3_SEQUENCE=TCCCCCGACACATACATACA
PRIMER_RIGHT_3_SEQUENCE=GCCATGCTAAGACACAGGCT
PRIMER_LEFT_3=13,20
PRIMER_RIGHT_3=158,20
PRIMER_LEFT_3_TM=59.648
PRIMER_RIGHT_3_TM=60.428
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=146
PRIMER_PAIR_3_PRODUCT_TM=68.4
PRIMER_PAIR_4_PENALTY=0.779333
PRIMER_LEFT_4_PENALTY=0.351810
PRIMER_RIGHT_4_PENALTY=0.427522
PRIMER_LEFT_4_SEQUENCE=TCCCCCGACACATACATACA
PRIMER_RIGHT_4_SEQUENCE=AGCCATGCTAAGACACAGGC
PRIMER_LEFT_4=13,20
PRIMER_RIGHT_4=159,20
PRIMER_LEFT_4_TM=59.648
PRIMER_RIGHT_4_TM=60.428
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=147
PRIMER_PAIR_4_PRODUCT_TM=68.4
=
SEQUENCE_ID=19.RHAP61FG1.seq
SEQUENCE_TEMPLATE=NNACNNACTTANTCCNNAAAGNANNACNNNNNNNNNNNNNNNNTGNCGNNNNNNNNNNNNNNNNNNNACCCATNNAGNCATACATACNGTCACATGTGTGTATANACANCNGNAGTCAACCNNGCNTCTTNTTCCNCAATTANANCNCCTNAANANNANNGNNNNNTGATAGGNCNATTACNTCNCCACTNACCTGNGTNNNNGCGGNCTCTNNANTTCGGGGNATCATTNNCTTGAACGGCNCNCCNNTGNTNAANNNTTTGGCNGNNGGNNGNTTNNCACNNANGATGNGTNNNNNGGNNNGCATNNNAGNTGTNAAGGCNTCGGGNGGGNNNGTATGGGAGGTGNGGNNNGTCNANNGGANGGNNTCGNNGGNGNNCANGTTCCNGNNGNNNGNNNNCNNGAANTNTTNNNGNNNNGGNNGGNGNGANGNNAGGATGNNAGNTCNNNNNGANNGTGNGNTTNGNNGNNTANGNGNNNCTGGGGGGGGNANAANNTTNNNGGGTGGTGTCGNTGGNNTGGNTGGTTTGNNTGCNTAGNNT
SEQUENCE_INCLUDED_REGION=0,542
SEQUENCE_TARGET=29,14
SEQUENCE_TARGET=49,18
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=20.RHAP61FG2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNTAACAACTGAGACAGAAGAAAATGTCAGAAATGAAAAAAATAAAAAAAGCAGAAAACTNACTNATCAACCGTAAAAACAAACCAACCAAAATGTAAATTAAAAATCTAAAACACTTATACGTGACATTCAGAAATGTCTGTCAATGACTGAGAATTATTCACATCTAAACAATGAAAGAACACACAGTGCAATTATATCTGTATTAAATGTTTCCAGTTTGGGAGATATGCATTGAAGTACCAATATTGATCAAGATCTTTCTTGCGGGTGTGTGAGTATTAAATATGAATGTGNNAACAGTATGTTAACATCTGAGAGCATNCTCCATCATTCTGTCACCTCAGGTTTCATAGAAGAAATNTCCTATTGNCATGTTGTAGACTNTTTGGGAAAGNGNTANNNGCACTTTNAATCCCAGNNTCACCCTCTCAGGTCNTGGGATCTGNNCCACNGCCGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGT
SEQUENCE_INCLUDED_REGION=12,509
SEQUENCE_TARGET=475,37
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=21.RHAP61FG9.seq
SEQUENCE_TEMPLATE=AATGANTTAGAGGACCCCTTCCTCACAGGTCCAGAAGGACACTGAAGAANAGGTTAGCCAGATATGCTAAGGCTCTTGTCACTAGTCAGTTCTGAATTGCTTCCCTTTAATGTCTTGTCCGGGAAGGGACTGAGAATCCANTCANACATGTCTACCNTGNGNCTCNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTNTCTCTCTCCTCAACCCAACCCAAACCACATTTCCCCCCCACACNNTCCCTCATATCNCCTTGNTTGNNATTCCCCCCCCCCCCCNACTNANANNTACNCNTNNNTTNTTTNAANNANAAAACCNCTNNNAAAAAAAACCNNNNGTAGNNNNNANANCNAAAATTTNTAAANANGNNNNNNG
SEQUENCE_INCLUDED_REGION=0,479
SEQUENCE_TARGET=169,44
SEQUENCE_TARGET=215,40
SEQUENCE_TARGET=262,30
P3_COMMENT=CL is TAATGTCTTGTCCGGGAAGG
P3_COMMENT=CR is GGTTGGGTTGAGGAGAGAGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.846305
PRIMER_LEFT_0_PENALTY=0.074208
PRIMER_RIGHT_0_PENALTY=0.772097
PRIMER_LEFT_0_SEQUENCE=TAATGTCTTGTCCGGGAAGG
PRIMER_RIGHT_0_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
PRIMER_LEFT_0=107,20
PRIMER_RIGHT_0=317,20
PRIMER_LEFT_0_TM=59.926
PRIMER_RIGHT_0_TM=59.228
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=211
PRIMER_PAIR_0_PRODUCT_TM=65.4
PRIMER_PAIR_1_PENALTY=1.001278
PRIMER_LEFT_1_PENALTY=0.229181
PRIMER_RIGHT_1_PENALTY=0.772097
PRIMER_LEFT_1_SEQUENCE=TTCCTCACAGGTCCAGAAGG
PRIMER_RIGHT_1_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
PRIMER_LEFT_1=18,20
PRIMER_RIGHT_1=317,20
PRIMER_LEFT_1_TM=60.229
PRIMER_RIGHT_1_TM=59.228
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=300
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=1.001278
PRIMER_LEFT_2_PENALTY=0.229181
PRIMER_RIGHT_2_PENALTY=0.772097
PRIMER_LEFT_2_SEQUENCE=CCTTCCTCACAGGTCCAGAA
PRIMER_RIGHT_2_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
PRIMER_LEFT_2=16,20
PRIMER_RIGHT_2=317,20
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=59.228
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=302
PRIMER_PAIR_2_PRODUCT_TM=69.5
PRIMER_PAIR_3_PENALTY=1.090008
PRIMER_LEFT_3_PENALTY=0.317911
PRIMER_RIGHT_3_PENALTY=0.772097
PRIMER_LEFT_3_SEQUENCE=CCTCACAGGTCCAGAAGGAC
PRIMER_RIGHT_3_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
PRIMER_LEFT_3=20,20
PRIMER_RIGHT_3=317,20
PRIMER_LEFT_3_TM=59.682
PRIMER_RIGHT_3_TM=59.228
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=298
PRIMER_PAIR_3_PRODUCT_TM=69.3
PRIMER_PAIR_4_PENALTY=1.094878
PRIMER_LEFT_4_PENALTY=0.074208
PRIMER_RIGHT_4_PENALTY=1.020670
PRIMER_LEFT_4_SEQUENCE=TAATGTCTTGTCCGGGAAGG
PRIMER_RIGHT_4_SEQUENCE=GGGTTGGGTTGAGGAGAGAG
PRIMER_LEFT_4=107,20
PRIMER_RIGHT_4=318,20
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=61.021
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=212
PRIMER_PAIR_4_PRODUCT_TM=65.6
=
SEQUENCE_ID=24.RHAP61FH3.seq
SEQUENCE_TEMPLATE=TAGAAATGNGACTGGTGAGATTTNANATTGTCCAANCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATTTCAAACATTTCTGTGTGTGTTTCTCCAGATCTTCCTCTTTCTCTTCCTATACACATGAGAAAAGTANAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGCGGCGCGACACACACANATGGACAAATTGGNAAGANAGAAAGGATTNANTNNTTTAGACAACAGGTGTNNGAGATCNGGATTTTTCTCNTANANNTGTNGNCTGTTGNNAGGTNNCCAGGTGANTAGGGATNGGTGNNGANTTANNGGGGGGAGGTGCGNGGTNTNNCAGNNNNTTNATGGNTGGNGNTNGGTGAANNANANNTTCTCAGGNNNANNGNNGGGGGACGNATGTGGTTGGNNNNNNANNNNNANNNNNNTNTTGCNGNNGGNNNNNNGGNGNNTGNNNNTTTGNNTGANGGNNGGGGNGNNCNNCAAT
SEQUENCE_INCLUDED_REGION=0,529
SEQUENCE_TARGET=43,42
SEQUENCE_TARGET=162,44
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=26.RHAP61FA11.seq
SEQUENCE_TEMPLATE=TNCCANCNTCCNATGCACCCACNCNGCCCCAGGGAANNACCGNNNACNCCCGNCTTCCNGNNCCCCATCNTCTGGCACGTNNACCCGNGNANATGCNNNNNNNNNNNNNNNNNCCCNNACNNACACACTNNAAATCNNTANAANANGNNTTNNATTCAGATAATACTATNNNCCNTGNTTNACACTTNCNNNNNCGNCATANTGCANATAACATGTTATGTAGGCATNTTGTCTGATGNAGNGGNACATGGCCCAGGCCCNAGTTNGGATCCTTAGNCACGNNTNANAGGNNNTGGGNATGNTNATTCNGCCCACAGGTNTTGGGNTCGTCANNANGGGGTACAGANNGNGANTTNGCNANNATGNNNATAAGGATGGNNNCNTGTGCGNNNTTGNTANNGNNANNNNTTTNCCAGGNNNANTANGGNGGGGGNNNNNTNNAGTGTNANT
SEQUENCE_INCLUDED_REGION=0,450
SEQUENCE_TARGET=99,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=26.RHAP61FA5.seq
SEQUENCE_TEMPLATE=CTGTGTGAATTTCTAAGTCTTACCTTCAGAAGCAGTACCAGGTNGACATGAAAATACTGTCACTCTCTCAAATCATGTAATATTTCTGTTTGACTTGACATATGCAGAGAGTCTCTGTTTCAGCATAAGACATGCTAGCAGGCTTAAAAATACTGTTTCTGGCTGCTAAATCCTGAATCTACAAATGTGGGATCAATGATGTGAAAAGAGAAACTAAATGATGTGTGGAAGTTTATCAACTCACAGTGACAAGAAGCAGACTGCAGCAAGGTACCACACATTCAGGAAGTGAATGACTCCTTAAGTNATTCTCCTATAGAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNTGNGATNTATNNGCCATGAATGTTTNTGNNTCTAGATACAAGAAAAGGGNTGGGTATTTNTGNGGCTCCNACAAGGGGCTTANTCACCAANTTTCTTTCNNGNNGAGGGGTTNCGGCNCGNATTT
SEQUENCE_INCLUDED_REGION=0,508
SEQUENCE_TARGET=324,56
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=27.RHAP61FA12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTCTGATGGAGCCTGTAGTCTAGCAGTATGTTTTCCTGAGACAAAAAAAGACACAGAAAAANATTGGAAGATTTAGCAATATGGAAGGATGCTGAAACCACAGGTATTCTTGGAATTCTTTCACTTACCTTAGTATGCCCCTTGTGNGGATTGAATGNNNTCCTGNAATACAGGCTGTCCTAGATTACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGGTTTTGGAGATGAAAAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,258
SEQUENCE_TARGET=213,42
P3_COMMENT=CL is AAGGATGCTGAAACCACAGG
P3_COMMENT=CR is TCCTTTTCATCTCCAAAACCTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.000620
PRIMER_LEFT_0_PENALTY=0.111077
PRIMER_RIGHT_0_PENALTY=2.889543
PRIMER_LEFT_0_SEQUENCE=AAGGATGCTGAAACCACAGG
PRIMER_RIGHT_0_SEQUENCE=TCCTTTTCATCTCCAAAACCTT
PRIMER_LEFT_0=105,20
PRIMER_RIGHT_0=278,22
PRIMER_LEFT_0_TM=60.111
PRIMER_RIGHT_0_TM=59.110
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=68.9
PRIMER_PAIR_1_PENALTY=3.310315
PRIMER_LEFT_1_PENALTY=0.420772
PRIMER_RIGHT_1_PENALTY=2.889543
PRIMER_LEFT_1_SEQUENCE=AGGATGCTGAAACCACAGGT
PRIMER_RIGHT_1_SEQUENCE=TCCTTTTCATCTCCAAAACCTT
PRIMER_LEFT_1=106,20
PRIMER_RIGHT_1=278,22
PRIMER_LEFT_1_TM=59.579
PRIMER_RIGHT_1_TM=59.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=173
PRIMER_PAIR_1_PRODUCT_TM=69.0
PRIMER_PAIR_2_PENALTY=3.338080
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=3.227003
PRIMER_LEFT_2_SEQUENCE=AAGGATGCTGAAACCACAGG
PRIMER_RIGHT_2_SEQUENCE=TCCTTTTCATCTCCAAAACCT
PRIMER_LEFT_2=105,20
PRIMER_RIGHT_2=278,21
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=57.773
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=174
PRIMER_PAIR_2_PRODUCT_TM=68.9
PRIMER_PAIR_3_PENALTY=3.431150
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=3.320073
PRIMER_LEFT_3_SEQUENCE=AAGGATGCTGAAACCACAGG
PRIMER_RIGHT_3_SEQUENCE=CCTTTTCATCTCCAAAACCTTTC
PRIMER_LEFT_3=105,20
PRIMER_RIGHT_3=277,23
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=60.320
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=173
PRIMER_PAIR_3_PRODUCT_TM=69.0
PRIMER_PAIR_4_PENALTY=3.431150
PRIMER_LEFT_4_PENALTY=0.111077
PRIMER_RIGHT_4_PENALTY=3.320073
PRIMER_LEFT_4_SEQUENCE=AAGGATGCTGAAACCACAGG
PRIMER_RIGHT_4_SEQUENCE=TCCTTTTCATCTCCAAAACCTTT
PRIMER_LEFT_4=105,20
PRIMER_RIGHT_4=278,23
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=60.320
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=68.9
=
SEQUENCE_ID=27.RHAP61FA6.seq
SEQUENCE_TEMPLATE=GTAAAATGCGTANNAACCAGCACAANCAACACACTGTTTGAAGGCAGGAGAATTTTCTCTCATTCTGAGGAACATAAACTNTTTATTATGNACAGGCCTTTGGTAGAACCAGTATAATGTTTAATCAAATATTTGGGCATCTCATGCTCTGTGAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCCCACAGACATCTAGAAGAATCTAGACAAGATAACAGAACATTNTCAACTAACTGAACCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,281
SEQUENCE_TARGET=158,56
P3_COMMENT=CL is CAGGCCTTTGGTAGAACCAG
P3_COMMENT=CR is TTCTTCTAGATGTCTGTGGGATG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.940409
PRIMER_LEFT_0_PENALTY=0.271680
PRIMER_RIGHT_0_PENALTY=4.668729
PRIMER_LEFT_0_SEQUENCE=CAGGCCTTTGGTAGAACCAG
PRIMER_RIGHT_0_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG
PRIMER_LEFT_0=92,20
PRIMER_RIGHT_0=236,23
PRIMER_LEFT_0_TM=59.728
PRIMER_RIGHT_0_TM=58.331
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=145
PRIMER_PAIR_0_PRODUCT_TM=65.7
PRIMER_PAIR_1_PENALTY=5.076670
PRIMER_LEFT_1_PENALTY=0.407941
PRIMER_RIGHT_1_PENALTY=4.668729
PRIMER_LEFT_1_SEQUENCE=ACAGGCCTTTGGTAGAACCA
PRIMER_RIGHT_1_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG
PRIMER_LEFT_1=91,20
PRIMER_RIGHT_1=236,23
PRIMER_LEFT_1_TM=59.592
PRIMER_RIGHT_1_TM=58.331
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=146
PRIMER_PAIR_1_PRODUCT_TM=65.6
PRIMER_PAIR_2_PENALTY=5.599600
PRIMER_LEFT_2_PENALTY=0.271680
PRIMER_RIGHT_2_PENALTY=5.327920
PRIMER_LEFT_2_SEQUENCE=CAGGCCTTTGGTAGAACCAG
PRIMER_RIGHT_2_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG
PRIMER_LEFT_2=92,20
PRIMER_RIGHT_2=237,24
PRIMER_LEFT_2_TM=59.728
PRIMER_RIGHT_2_TM=58.672
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=146
PRIMER_PAIR_2_PRODUCT_TM=65.6
PRIMER_PAIR_3_PENALTY=5.645139
PRIMER_LEFT_3_PENALTY=0.271680
PRIMER_RIGHT_3_PENALTY=5.373459
PRIMER_LEFT_3_SEQUENCE=CAGGCCTTTGGTAGAACCAG
PRIMER_RIGHT_3_SEQUENCE=GATTCTTCTAGATGTCTGTGGGATG
PRIMER_LEFT_3=92,20
PRIMER_RIGHT_3=238,25
PRIMER_LEFT_3_TM=59.728
PRIMER_RIGHT_3_TM=60.373
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=44.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=147
PRIMER_PAIR_3_PRODUCT_TM=65.9
PRIMER_PAIR_4_PENALTY=5.735861
PRIMER_LEFT_4_PENALTY=0.407941
PRIMER_RIGHT_4_PENALTY=5.327920
PRIMER_LEFT_4_SEQUENCE=ACAGGCCTTTGGTAGAACCA
PRIMER_RIGHT_4_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG
PRIMER_LEFT_4=91,20
PRIMER_RIGHT_4=237,24
PRIMER_LEFT_4_TM=59.592
PRIMER_RIGHT_4_TM=58.672
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=147
PRIMER_PAIR_4_PRODUCT_TM=65.6
=
SEQUENCE_ID=29.RHAP61FB11.seq
SEQUENCE_TEMPLATE=GGCNNTATAGANNATCCCNTCNGGNCATNTCAANCCCACATNCTCTTANAANAACTTTGNAAAATGATGNNTGANNAAAANTTACTGCTNNNGNNNNNTCNACTTTGGNGNGNCTCTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATANGTGTCNATTTANNATGCANANCTNNNTCGGAGGTTGTGNTGNNCNCTCNAAAACTCNTTNTNNNTNCNTCCAGGGGNNGGGNNCNNCCGNNTNNNTCTCGNNCNTTTNNANATANGNANGTAANTCTGGNATCNTCCNANTNNACNNNTNAGNGAANNATGGGTNNNNGNAAGNGCTTTTNTNNNANGAGGGGGGGNGNNNNNGANNTGGAATGGGNANNNNGGGNNNAAAANNNGTTTTCCNTTGGNAANTTNNNNTCNNNNNNGANTNNNANAANAANATTNGAGNGGGAANCNNNAANNNTAAAGCNNGGGGNNCTT
SEQUENCE_INCLUDED_REGION=0,490
SEQUENCE_TARGET=123,40
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=30.RHAP61FB12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTGCCTGAAAAAAGAAAATCACACTTTTTGATCTCTAGCCACCTAAAATGTTTGCTAAGTTTCACTTCCCAACACCAGGGGGTGTCTCTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCCTTAAGGTTCCATTAGTCCCAAACCTTTCTCCGTGTTTTTATGTACACTCTAAATCTTTCTCTGTCCCTCCAGAGGCAGGGATCAGCCTTATTTTCCTCGCTCTTTTTCATATAAGCACATAAATCTTGTATCCCCCCACTCCACCNNCAGAGAACCATGGGTCTAGAAAGTGCTTTTTATTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,332
SEQUENCE_TARGET=112,46
P3_COMMENT=CL is AACACCAGGGGGTGTCTCTT
P3_COMMENT=CR is AATAAGGCTGATCCCTGCCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.861541
PRIMER_LEFT_0_PENALTY=0.801345
PRIMER_RIGHT_0_PENALTY=0.060196
PRIMER_LEFT_0_SEQUENCE=AACACCAGGGGGTGTCTCTT
PRIMER_RIGHT_0_SEQUENCE=AATAAGGCTGATCCCTGCCT
PRIMER_LEFT_0=88,20
PRIMER_RIGHT_0=257,20
PRIMER_LEFT_0_TM=60.801
PRIMER_RIGHT_0_TM=60.060
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=170
PRIMER_PAIR_0_PRODUCT_TM=69.9
PRIMER_PAIR_1_PENALTY=1.156250
PRIMER_LEFT_1_PENALTY=1.096054
PRIMER_RIGHT_1_PENALTY=0.060196
PRIMER_LEFT_1_SEQUENCE=CAGGGGGTGTCTCTTCGTT
PRIMER_RIGHT_1_SEQUENCE=AATAAGGCTGATCCCTGCCT
PRIMER_LEFT_1=93,19
PRIMER_RIGHT_1=257,20
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=69.7
PRIMER_PAIR_2_PENALTY=1.220450
PRIMER_LEFT_2_PENALTY=0.801345
PRIMER_RIGHT_2_PENALTY=0.419105
PRIMER_LEFT_2_SEQUENCE=AACACCAGGGGGTGTCTCTT
PRIMER_RIGHT_2_SEQUENCE=AAATAAGGCTGATCCCTGCC
PRIMER_LEFT_2=88,20
PRIMER_RIGHT_2=258,20
PRIMER_LEFT_2_TM=60.801
PRIMER_RIGHT_2_TM=60.419
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=69.8
PRIMER_PAIR_3_PENALTY=1.368277
PRIMER_LEFT_3_PENALTY=0.801345
PRIMER_RIGHT_3_PENALTY=0.566932
PRIMER_LEFT_3_SEQUENCE=AACACCAGGGGGTGTCTCTT
PRIMER_RIGHT_3_SEQUENCE=ATAAGGCTGATCCCTGCCTC
PRIMER_LEFT_3=88,20
PRIMER_RIGHT_3=256,20
PRIMER_LEFT_3_TM=60.801
PRIMER_RIGHT_3_TM=60.567
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=169
PRIMER_PAIR_3_PRODUCT_TM=69.9
PRIMER_PAIR_4_PENALTY=1.423937
PRIMER_LEFT_4_PENALTY=1.363742
PRIMER_RIGHT_4_PENALTY=0.060196
PRIMER_LEFT_4_SEQUENCE=ACACCAGGGGGTGTCTCTTC
PRIMER_RIGHT_4_SEQUENCE=AATAAGGCTGATCCCTGCCT
PRIMER_LEFT_4=89,20
PRIMER_RIGHT_4=257,20
PRIMER_LEFT_4_TM=61.364
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=169
PRIMER_PAIR_4_PRODUCT_TM=69.9
=
SEQUENCE_ID=31.RHAP61FC4.seq
SEQUENCE_TEMPLATE=CCTNCCCCNCNNCNNTGGNCCCNCTCAANNNNNNNNNNNNNNNNNNTCTNCANNNAGNNCGNNACCNTCNATCNCNAGNCANGNTCCNTCCCNNNAACNANNNTCNTCTATAGAAAACNCNCTGGNCGCNCTACGATNNGNNCNNACNNTNNNANNNTNGNNNTNNAANGGNCNNNNNNNGCNNCGNNGNNCNNACNNNCNNNTNNANNTNNCTNNNTNNNNTCGNNNNGNNCNNTNGNNNNGNNNCNNGCTNCNTGGCTTNNNANNNNNCCGACNNNNNNACCNTTTNCNNTNTTTNNGCNTGCNNCNCGNNCCTCNGGNNNNNNNGNNNNNNNNNTNNNGNTTNCNNNCGNGNNGGCNNNNTNNNNNGNNNNGNNNGNNNNGNTCGGNNNCCGNTNNGNNNNCCCNNCCACNNNCCGNTCGNNNNNCNCNNNCNNGCNNCNACCNCNCGGNNNNCNNNGNNNCNNNCNNCNCNGNNTGGNGCGNNCGGCNNNCNGNNGNTGNNNNNNNCNNCCNGNNNNNNNGNTCNNNNGTNGGGNNTCCNNNCCNNTNCNNNNNNNNNCNNNCNGNNNCGNNNGGC
SEQUENCE_INCLUDED_REGION=0,580
SEQUENCE_TARGET=29,17
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=33.RHAP61FC12.seq
SEQUENCE_TEMPLATE=TCCACATNNCAAGTTCCAGGTCAACCAGGGTTNNGTGGTGAGACCTCCCCCTTCTCTTACGCACACGCCAAACCTTAACAAACCCCCTAAAAGCCAAACCNTGAGAAAACAGAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNTCANAGTCNCTTCNAGANTTGCATACCTAATATGNGCCNTTATCTATGTNNGCAAATGAANGTGAGTCTATANTTTNTNATACCANNGGGGGAGCNTTTGGGCNGCCCAGGGAGCTTTGGTGGGAGAGNATANAGNGGGGNGNNCNNNTCNTGGNAANNAGGAAATCTCNTTNNGNNNNTTNNTGNTANGTCCNGGAGTNTGTGTATNNNATCCTTNNGNTTTNTNNTNNGANATCNNTCCNCTAGGGNTCTTGGTCNNGGGTCNNNGTNNNNNNCGGGTGGAAAAT
SEQUENCE_INCLUDED_REGION=0,469
SEQUENCE_TARGET=118,60
P3_COMMENT=CL is GACCTCCCCCTTCTCTTACG
P3_COMMENT=CR is CTCCCACCAAAGCTCCCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.257636
PRIMER_LEFT_0_PENALTY=0.066676
PRIMER_RIGHT_0_PENALTY=2.190960
PRIMER_LEFT_0_SEQUENCE=GACCTCCCCCTTCTCTTACG
PRIMER_RIGHT_0_SEQUENCE=CTCCCACCAAAGCTCCCT
PRIMER_LEFT_0=41,20
PRIMER_RIGHT_0=307,18
PRIMER_LEFT_0_TM=60.067
PRIMER_RIGHT_0_TM=60.191
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.7000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=267
PRIMER_PAIR_0_PRODUCT_TM=71.2
PRIMER_PAIR_1_PENALTY=2.376903
PRIMER_LEFT_1_PENALTY=0.185942
PRIMER_RIGHT_1_PENALTY=2.190960
PRIMER_LEFT_1_SEQUENCE=TGAGACCTCCCCCTTCTCTT
PRIMER_RIGHT_1_SEQUENCE=CTCCCACCAAAGCTCCCT
PRIMER_LEFT_1=38,20
PRIMER_RIGHT_1=307,18
PRIMER_LEFT_1_TM=60.186
PRIMER_RIGHT_1_TM=60.191
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=270
PRIMER_PAIR_1_PRODUCT_TM=71.2
PRIMER_PAIR_2_PENALTY=2.529770
PRIMER_LEFT_2_PENALTY=0.338810
PRIMER_RIGHT_2_PENALTY=2.190960
PRIMER_LEFT_2_SEQUENCE=GTGAGACCTCCCCCTTCTCT
PRIMER_RIGHT_2_SEQUENCE=CTCCCACCAAAGCTCCCT
PRIMER_LEFT_2=37,20
PRIMER_RIGHT_2=307,18
PRIMER_LEFT_2_TM=59.661
PRIMER_RIGHT_2_TM=60.191
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=271
PRIMER_PAIR_2_PRODUCT_TM=71.3
PRIMER_PAIR_3_PENALTY=2.564318
PRIMER_LEFT_3_PENALTY=0.373358
PRIMER_RIGHT_3_PENALTY=2.190960
PRIMER_LEFT_3_SEQUENCE=CACACGCCAAACCTTAACAA
PRIMER_RIGHT_3_SEQUENCE=CTCCCACCAAAGCTCCCT
PRIMER_LEFT_3=61,20
PRIMER_RIGHT_3=307,18
PRIMER_LEFT_3_TM=59.627
PRIMER_RIGHT_3_TM=60.191
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=247
PRIMER_PAIR_3_PRODUCT_TM=70.1
PRIMER_PAIR_4_PENALTY=2.625703
PRIMER_LEFT_4_PENALTY=0.434743
PRIMER_RIGHT_4_PENALTY=2.190960
PRIMER_LEFT_4_SEQUENCE=CCAAACCTTAACAAACCCCC
PRIMER_RIGHT_4_SEQUENCE=CTCCCACCAAAGCTCCCT
PRIMER_LEFT_4=67,20
PRIMER_RIGHT_4=307,18
PRIMER_LEFT_4_TM=60.435
PRIMER_RIGHT_4_TM=60.191
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=241
PRIMER_PAIR_4_PRODUCT_TM=69.7
=
SEQUENCE_ID=34.RHAP61FD10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCTGGAGGATTTCTTTTTCTCTGATAGTATTTAATGTTTCTTTTTCTCTTCTTCAATTGAGGTTCCCTTTTCCTGTGTAAGTCAGAGTTCTTATTTCCACTTCAGTCTCTCAGACAGTTTCGATTACANNNNNNNNNNNNNNNNCCACTTAGATTTCTATTCAGGACAGAAGTCAAATTACTTTACTCGGCTGTTAATGCTTTTCAATTGCACAGAAAAAGGCTCTAGATCTTTGGTACCGAGTTTGCTAACCAATGTGACAGACCCTTTGTTTTAAAATTATGAACGGGGGAAAACAAATAGAGGAAATAAATTCNNAATAGCATGAATTTATACTTCCNGAAATGACCAAAGGGAAAGCCAGGTCCCATNATTACCTTGNGATGCAGAGCAGCNGGNTCAGNACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,411
SEQUENCE_TARGET=146,16
P3_COMMENT=CL is TGAGGTTCCCTTTTCCTGTG
P3_COMMENT=CR is TGAAAAGCATTAACAGCCGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.637239
PRIMER_LEFT_0_PENALTY=0.080729
PRIMER_RIGHT_0_PENALTY=0.556510
PRIMER_LEFT_0_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
PRIMER_RIGHT_0_SEQUENCE=TGAAAAGCATTAACAGCCGA
PRIMER_LEFT_0=75,20
PRIMER_RIGHT_0=223,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=59.443
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=11.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=149
PRIMER_PAIR_0_PRODUCT_TM=69.9
PRIMER_PAIR_1_PENALTY=1.123197
PRIMER_LEFT_1_PENALTY=0.080729
PRIMER_RIGHT_1_PENALTY=1.042469
PRIMER_LEFT_1_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
PRIMER_RIGHT_1_SEQUENCE=TTGAAAAGCATTAACAGCCG
PRIMER_LEFT_1=75,20
PRIMER_RIGHT_1=224,20
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=58.958
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=150
PRIMER_PAIR_1_PRODUCT_TM=69.8
PRIMER_PAIR_2_PENALTY=1.186120
PRIMER_LEFT_2_PENALTY=0.080729
PRIMER_RIGHT_2_PENALTY=1.105391
PRIMER_LEFT_2_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
PRIMER_RIGHT_2_SEQUENCE=GCCTTTTTCTGTGCAATTGA
PRIMER_LEFT_2=75,20
PRIMER_RIGHT_2=240,20
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=58.895
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=70.6
PRIMER_PAIR_3_PENALTY=1.205774
PRIMER_LEFT_3_PENALTY=0.080729
PRIMER_RIGHT_3_PENALTY=1.125045
PRIMER_LEFT_3_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
PRIMER_RIGHT_3_SEQUENCE=AGCCTTTTTCTGTGCAATTGA
PRIMER_LEFT_3=75,20
PRIMER_RIGHT_3=241,21
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=70.5
PRIMER_PAIR_4_PENALTY=1.205774
PRIMER_LEFT_4_PENALTY=0.080729
PRIMER_RIGHT_4_PENALTY=1.125045
PRIMER_LEFT_4_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
PRIMER_RIGHT_4_SEQUENCE=GAGCCTTTTTCTGTGCAATTG
PRIMER_LEFT_4=75,20
PRIMER_RIGHT_4=242,21
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=168
PRIMER_PAIR_4_PRODUCT_TM=70.7
=
SEQUENCE_ID=36.RHAP61FD12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCTCAGCCCGTGTCATTTGACGCTTTCTTAGCACAATATTCCAGACTCTTTCACCTTTTGGTTATGATATAGTTCAAGCAGTTAAAAATCACGGAGGTTCATCACAGAAAGGGTGATGCTTGCTTTTTTTGGTACCAAGTTCTANCNTTGTTTAATTTCTTTGACCTTCCTCTACAATCTCATTTATTTTCAGTCAGGCGAGTGTCTGTGAGTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCACTCATGTGCATACACACAGACGTTAGAAAAAGCCAGTTCCCCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=20,315
SEQUENCE_TARGET=236,48
P3_COMMENT=CL is TTCAGTCAGGCGAGTGTCTG
P3_COMMENT=CR is TCTAGGGGAACTGGCTTTTTC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.466551
PRIMER_LEFT_0_PENALTY=0.178378
PRIMER_RIGHT_0_PENALTY=1.288173
PRIMER_LEFT_0_SEQUENCE=TTCAGTCAGGCGAGTGTCTG
PRIMER_RIGHT_0_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
PRIMER_LEFT_0=207,20
PRIMER_RIGHT_0=334,21
PRIMER_LEFT_0_TM=60.178
PRIMER_RIGHT_0_TM=59.712
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=128
PRIMER_PAIR_0_PRODUCT_TM=67.7
PRIMER_PAIR_1_PENALTY=1.514665
PRIMER_LEFT_1_PENALTY=0.794652
PRIMER_RIGHT_1_PENALTY=0.720013
PRIMER_LEFT_1_SEQUENCE=GGGTGATGCTTGCTTTTTTT
PRIMER_RIGHT_1_SEQUENCE=GTGTATGCACATGAGTGCGA
PRIMER_LEFT_1=129,20
PRIMER_RIGHT_1=303,20
PRIMER_LEFT_1_TM=59.205
PRIMER_RIGHT_1_TM=59.280
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=68.4
PRIMER_PAIR_2_PENALTY=1.684835
PRIMER_LEFT_2_PENALTY=0.396662
PRIMER_RIGHT_2_PENALTY=1.288173
PRIMER_LEFT_2_SEQUENCE=AGTCAGGCGAGTGTCTGTGA
PRIMER_RIGHT_2_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
PRIMER_LEFT_2=210,20
PRIMER_RIGHT_2=334,21
PRIMER_LEFT_2_TM=59.603
PRIMER_RIGHT_2_TM=59.712
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=125
PRIMER_PAIR_2_PRODUCT_TM=67.6
PRIMER_PAIR_3_PENALTY=1.684835
PRIMER_LEFT_3_PENALTY=0.396662
PRIMER_RIGHT_3_PENALTY=1.288173
PRIMER_LEFT_3_SEQUENCE=TCAGTCAGGCGAGTGTCTGT
PRIMER_RIGHT_3_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
PRIMER_LEFT_3=208,20
PRIMER_RIGHT_3=334,21
PRIMER_LEFT_3_TM=59.603
PRIMER_RIGHT_3_TM=59.712
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=67.8
PRIMER_PAIR_4_PENALTY=1.927370
PRIMER_LEFT_4_PENALTY=0.178378
PRIMER_RIGHT_4_PENALTY=1.748991
PRIMER_LEFT_4_SEQUENCE=TTCAGTCAGGCGAGTGTCTG
PRIMER_RIGHT_4_SEQUENCE=AGGGGAACTGGCTTTTTCTAA
PRIMER_LEFT_4=207,20
PRIMER_RIGHT_4=331,21
PRIMER_LEFT_4_TM=60.178
PRIMER_RIGHT_4_TM=59.251
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=125
PRIMER_PAIR_4_PRODUCT_TM=67.6
=
SEQUENCE_ID=36.RHAP61FD6.seq
SEQUENCE_TEMPLATE=GTCTGAGACCCAGCAANATGNGGGACAGAAATAGAAGACCGACCTGTAATTTCATGGACAAGTTAATCTGGAGTTTACAGTACAGCGGAAACAAAAGANNTCCCGCCTCAGCAAAGTGGAAAGGNGAGAACCAANTCCTGAAAATTATCCTCTGATCTCCATTAGTGTTCCATAGCACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNACATTNNNNNNNNNNNNNNTGCNANTNTCNCTTNCTNNAAAGANCAACNATGGNNGCACTTTCTCTCTTTAGCNGACCCANNGGNGCGGGTNNTGTNAACTCNNGNTTCTGNGGANNGGGGGTANCGGNGCNCGNNTTNGTTGNNCNTGGTCNTNGCNGTTTCCTTGTTGTGANNTTGTGNNTGCAGCGNNCNGTTCNANNNANNNTGGCGNGCCGGGAGNNTTNAGNGGAAANCCTGNNNGTTGCTTTNTGNNGGNGAGCTANGTCNCNTNNTTTGGGTNNNNNNNNNGGGNCNCTTTT
SEQUENCE_INCLUDED_REGION=0,530
SEQUENCE_TARGET=182,46
SEQUENCE_TARGET=235,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=38.RHAP61FE5.seq
SEQUENCE_TEMPLATE=NANACCNCCCCNANCCTGNCCTCTATCTCNNNCCNNNNNNNNNNNNNNNNNNNNTNCNTCCTCTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGAATTAATTNTAGGTGTGGTAAGCATTTGGTGCCAGCATTAATTTCCAGTCCCACCTTAAGAGNTTACTAGTGCGAANCTGAANTAANATTGACACAGTGAACGACGANTGCTGGGTAAGNGTCNNAGGGGNNCTTTGTNNTCACTCNCTACACTTAGGTGTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,320
SEQUENCE_TARGET=34,20
SEQUENCE_TARGET=69,82
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=39.RHAP61FE6.seq
SEQUENCE_TEMPLATE=NANNCNCCTAGAGGACCCTGTCCNGCCAAACATTGTNTTCATGTCATGGACAAGGACAGGGACAGGGACCNTNAAGTTNCTTTTNNCCTACAGGCNCTGCCAATCCTCTGCTTCTGTCATCCCCATAGCATGTCNTAGNGCTGGACATTACACACCNTTCANCGTNNNCNNCNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNGTNTNNCACAAGCGCCNGCTATCNTGANCCCNNCATAATNNTGGCCGNGTAGTANTCCNNANTGNTTATCTGCTNNCTATGTTTGTANGNNNGANAGNAGGATTNATGNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,370
SEQUENCE_TARGET=177,50
SEQUENCE_TARGET=230,25
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=41.RHAP61FF11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGTCTGTCAAAACTCCGGTCATCTTCGAGACTTGACACTGGTATTTTTGTGTATGGACCNTTTCCTAGTCACATAGACCTCATTCACAGTGGATANTANTGNGTCTCCCATCAAGTCCCTGAGTCACTGACCTAAGTACAGGCTGTATCATTGACACCATATTTNNTTCTGTTTCTTNNTCGTGGAAGTGNTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATGTGNGTGNGNTGTANNGNNNNNATNTNGTANNNNNTNNNNNAGANNNNAAAGGTCAANNNNNAGNNCNNTNTNTGNNAAAAANNNANNNNCNTTANTNTGANANAACTNTCNCNNAGAANGGGNNNCNNNNCNCNAATTCGCTNNNNANGNNAANAGNNNGNNNCNNNNNGNGAAANNNNNNNNCGCNCAAAAAANTNNANGAAAAANANG
SEQUENCE_INCLUDED_REGION=20,464
SEQUENCE_TARGET=216,50
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=41.RHAP61FF5.seq
SEQUENCE_TEMPLATE=TNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNTGNNATCCCNGGNGGTGACTGTGGGNGCATCNGTNANNGNGGNNGNGTNNNTGTNNCNAANNGTTGTGGGAGTCNNTNNTGTGNCCCTGGTGGNTGNAACTANNTGTAGNTGNTAAGAACACCNGTGGNGAGNTAAGAGNTCTNNCATGGACCGGGNANTNAGNTGGTAANCGNNCGCNAACNCGNNATCANNNNNANANNNNGTTTCCNNGNNNNAAAANNNNNANCCNNNGANNNTNNCNNNANANANTNGNNGNCCGGGGAANANNANGNNNNNANCNTGGGGGGTGNCNNTANGCCGNGAGNNNNNNNNNNANNNNNNTGGGCNNGNNNTCNNNTGNNNNGGNNGTCNANNNNNNNANANGNNTGNNGGNNCNAGNNGNNTNNNGNGAAAGGNNGNNAANNTGCGNGGANANGGNNNTTTGNNTANNGGGAGNATGNT
SEQUENCE_INCLUDED_REGION=0,494
SEQUENCE_TARGET=9,26
SEQUENCE_TARGET=37,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=42.RHAP61FF12.seq
SEQUENCE_TEMPLATE=NNNNNNTGTAATCCTGTAATCCAGGGACGTGCTTTTCTGTGTTAGTTGGAAATCTCCAGTAAGTAAAACCATGTGNGNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATAACTCCATCCATCCAATATCTAGTATACATNTATATACATTAGAGTTATTTATTACAAAGTACTGATGGACATGATTATNGANGCCAAGAAGTTCCAATTATTTTGAGTGAATTTCNNNAAAAGCCCANACTTANAANAGGAAAATAAAGCCTNTCTCCCTGTNGACAAANTGTCCNTGCGATAANCCTATAANCCCCNAACCCTTTTGGGAACAGTGCCAACCGNNNGGGGGNGGNAAAATTTCATATNNNTGNGGGCCCNNTGGGGNGTCANNTCTGTTNNAAGNNNCAATNNNNAAGGNNANNTTGNNANGGGGTGANCGNGGGNNTGGNNTTCGGNNTTNAANGGGGCNNNAGNTGTNTCCNGGT
SEQUENCE_INCLUDED_REGION=6,483
SEQUENCE_TARGET=82,32
P3_COMMENT=CL is CTGTAATCCAGGGACGTGCT
P3_COMMENT=CR is ACTAGATATTGGATGGATGGAGTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.958177
PRIMER_LEFT_0_PENALTY=0.133741
PRIMER_RIGHT_0_PENALTY=6.824436
PRIMER_LEFT_0_SEQUENCE=CTGTAATCCAGGGACGTGCT
PRIMER_RIGHT_0_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
PRIMER_LEFT_0=13,20
PRIMER_RIGHT_0=143,24
PRIMER_LEFT_0_TM=60.134
PRIMER_RIGHT_0_TM=57.176
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=131
PRIMER_PAIR_0_PRODUCT_TM=67.2
PRIMER_PAIR_1_PENALTY=7.255493
PRIMER_LEFT_1_PENALTY=0.431057
PRIMER_RIGHT_1_PENALTY=6.824436
PRIMER_LEFT_1_SEQUENCE=TAATCCAGGGACGTGCTTTT
PRIMER_RIGHT_1_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
PRIMER_LEFT_1=16,20
PRIMER_RIGHT_1=143,24
PRIMER_LEFT_1_TM=59.569
PRIMER_RIGHT_1_TM=57.176
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=128
PRIMER_PAIR_1_PRODUCT_TM=66.7
PRIMER_PAIR_2_PENALTY=7.341119
PRIMER_LEFT_2_PENALTY=0.516683
PRIMER_RIGHT_2_PENALTY=6.824436
PRIMER_LEFT_2_SEQUENCE=TGTAATCCAGGGACGTGCTT
PRIMER_RIGHT_2_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
PRIMER_LEFT_2=14,20
PRIMER_RIGHT_2=143,24
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=57.176
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=130
PRIMER_PAIR_2_PRODUCT_TM=67.0
PRIMER_PAIR_3_PENALTY=7.568314
PRIMER_LEFT_3_PENALTY=0.133741
PRIMER_RIGHT_3_PENALTY=7.434573
PRIMER_LEFT_3_SEQUENCE=CTGTAATCCAGGGACGTGCT
PRIMER_RIGHT_3_SEQUENCE=GATATTGGATGGATGGAGTTATAGA
PRIMER_LEFT_3=13,20
PRIMER_RIGHT_3=139,25
PRIMER_LEFT_3_TM=60.134
PRIMER_RIGHT_3_TM=57.565
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=36.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=67.1
PRIMER_PAIR_4_PENALTY=7.737137
PRIMER_LEFT_4_PENALTY=0.912701
PRIMER_RIGHT_4_PENALTY=6.824436
PRIMER_LEFT_4_SEQUENCE=TCCTGTAATCCAGGGACGTG
PRIMER_RIGHT_4_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
PRIMER_LEFT_4=11,20
PRIMER_RIGHT_4=143,24
PRIMER_LEFT_4_TM=60.913
PRIMER_RIGHT_4_TM=57.176
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=133
PRIMER_PAIR_4_PRODUCT_TM=67.4
=
SEQUENCE_ID=44.RHAP61FG5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNTATCTCTGACTTTGTTGTTTTGNTTTTCCGCANTTTTCATTGTGAATTCNATTTTTGNTTGNCTTTNATTTGCTGACNATCTCTTTATTAAANTTNANGTCTTGAATTGNTTCANTTATTTCACCCAACNANTCTNNNGGGGTANTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCNTATGGGGGTGNNTTTTACAGTCTCCCNNNAGANATNNATGGTCTATNNTCNNTAAGANCNNTGGACATANTCATAANNACNANTCAGTTAACANNGCNCCNNNAAATTAGNNNNTCNATNNANAATTGNNAGTGGNNAGTCCNTGGCCNGGGCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=10,395
SEQUENCE_TARGET=159,84
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=47.RHAP61FH11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTGTCAAAATTAAAAACCAGCAAGAAAGGTCACTTNNATTTTAAGTGGAGAGAGGAAATAATTNANGGATAAAATATAAAACAATAATGAAAAATTAATTTCATCATGGACTGCNTATTAAGANNNANNTGTACATAAGNGNTATTGTTGTGTCANNAGTTAACTTCCCNGNTANCCCGNACAGGNNNTNGGGTCTANNANNCNNNANGGCCNACNTNAANNTTAAATGNCANNTTGTTTTCNGGNGTGGNTTCAATGCNCTNTGNNNTNNNNCNCNNNGNNNNNNNNNNNNNNNNNNNNTGNTGNNNNNNNNNNNNNNNNNGNNNANAANNGGTNTNAANNANTTANGNTNNNNNGNNATNNGNGNNGNTNGTGTGNNNNNNGGTNNTGNTNGNGTGNTNCNNTGTTGNTNNNNNNNNNNTNNNNGANNGNATNNTGNCNAANNNNNNCGNNNNNNNNNNCTTNTTGNANNGNTNNGNATTGNNNNTTT
SEQUENCE_INCLUDED_REGION=19,489
SEQUENCE_TARGET=300,18
SEQUENCE_TARGET=323,16
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=48.RHAP61FH6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNTTGTGTCANACTNACAAAAATCNAACCAGGACACAATGTTNANCTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGTTTATGTCCTTTTCATCTCTTGTAGCATTTAGTATGCACTGAGGCCATATCTTTTGAAGTGAGAGTTATACTGACATATTAGTATAAATTTCTTTTTGCATCTCTGNAGTTTTATAGGATTGTGTGTANTGTAGAATCTAACAAACTTTANTAAGTCTAGCATCTCACCTAAAGGAAAGNNCTCTGGGGGCCTACACTGGGGTGTGACCAAACTGCCTGGCACCAGTAGGCGCTCTCCATGGGGGGNCATTCTTCAGGTACAATAAGGGGTTGTCTGTTCAATACAGCNGTGGGGGGAACNTGCTGGCTGATAAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=10,433
SEQUENCE_TARGET=58,62
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=01.RHAP64FA1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCAACGGGNTGGCTCAGTGGNTAGAGGTACTTGCCCACCNAGTTTTAACAACTGGAGTTCGGATCCCAGGGTCCCACGTCATGGAAGACAGGGAAAGNNTCCCACANTGGTTTACACACACTNCNTCGCACACAGTCACGCGCANGCGNAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGAGCACCAGAGGACGACAGAGGACAACAGAGAGCTNNNCNGTCACAGNCTCCNGACNNNNNNGTAATTAAGANNTTTGAGNAGNNNCNNNNGTGTTNGNTGNCAAAGAANAGNGNGGNNNTTNNNNNGNNAGTGGTGNANAGGNANNTTGGNCNGGNANGGAGGGCNNNNCCNTGGNGGNNGGNTNNNAAGNNNNNTGCNGNNNGANNGGNGNNNCGTGGNTGGGNNNGNANACNAGNNNNNNNANNNNNNTTGNNNNNGNNNTNNNNANCCNGGNGTNNNGAGNANNNGTTNNNNNNGAGNGNNNGTNNNC
SEQUENCE_INCLUDED_REGION=25,495
SEQUENCE_TARGET=176,28
P3_COMMENT=CL is ACGTCATGGAAGACAGGGAA
P3_COMMENT=CR is CTCTGTCGTCCTCTGGTGCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.118415
PRIMER_LEFT_0_PENALTY=0.510331
PRIMER_RIGHT_0_PENALTY=0.608084
PRIMER_LEFT_0_SEQUENCE=ACGTCATGGAAGACAGGGAA
PRIMER_RIGHT_0_SEQUENCE=CTCTGTCGTCCTCTGGTGCT
PRIMER_LEFT_0=99,20
PRIMER_RIGHT_0=228,20
PRIMER_LEFT_0_TM=60.510
PRIMER_RIGHT_0_TM=60.608
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=130
PRIMER_PAIR_0_PRODUCT_TM=72.0
PRIMER_PAIR_1_PENALTY=1.236221
PRIMER_LEFT_1_PENALTY=0.510331
PRIMER_RIGHT_1_PENALTY=0.725890
PRIMER_LEFT_1_SEQUENCE=ACGTCATGGAAGACAGGGAA
PRIMER_RIGHT_1_SEQUENCE=GTCCTCTGTCGTCCTCTGGT
PRIMER_LEFT_1=99,20
PRIMER_RIGHT_1=231,20
PRIMER_LEFT_1_TM=60.510
PRIMER_RIGHT_1_TM=59.274
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=133
PRIMER_PAIR_1_PRODUCT_TM=72.3
PRIMER_PAIR_2_PENALTY=1.546072
PRIMER_LEFT_2_PENALTY=0.510331
PRIMER_RIGHT_2_PENALTY=1.035741
PRIMER_LEFT_2_SEQUENCE=ACGTCATGGAAGACAGGGAA
PRIMER_RIGHT_2_SEQUENCE=TTGTCCTCTGTCGTCCTCTG
PRIMER_LEFT_2=99,20
PRIMER_RIGHT_2=233,20
PRIMER_LEFT_2_TM=60.510
PRIMER_RIGHT_2_TM=58.964
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=135
PRIMER_PAIR_2_PRODUCT_TM=72.2
PRIMER_PAIR_3_PENALTY=1.615970
PRIMER_LEFT_3_PENALTY=1.007886
PRIMER_RIGHT_3_PENALTY=0.608084
PRIMER_LEFT_3_SEQUENCE=AACAACTGGAGTTCGGATCC
PRIMER_RIGHT_3_SEQUENCE=CTCTGTCGTCCTCTGGTGCT
PRIMER_LEFT_3=70,20
PRIMER_RIGHT_3=228,20
PRIMER_LEFT_3_TM=58.992
PRIMER_RIGHT_3_TM=60.608
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=159
PRIMER_PAIR_3_PRODUCT_TM=74.2
PRIMER_PAIR_4_PENALTY=1.618571
PRIMER_LEFT_4_PENALTY=0.510331
PRIMER_RIGHT_4_PENALTY=1.108240
PRIMER_LEFT_4_SEQUENCE=ACGTCATGGAAGACAGGGAA
PRIMER_RIGHT_4_SEQUENCE=GTTGTCCTCTGTCGTCCTCTG
PRIMER_LEFT_4=99,20
PRIMER_RIGHT_4=234,21
PRIMER_LEFT_4_TM=60.510
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PAIR_4_PRODUCT_SIZE=136
PRIMER_PAIR_4_PRODUCT_TM=72.4
=
SEQUENCE_ID=01.RHAP64FA7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCACANAAAACATCCTGTCNNNNNNNNNNNNNNNNTAGCTNNNNNNNNNNNNNNNNTATAAGTGCATGGAATGAGTGTGTATANNNNNNNNNNNNNNNNNNNNNNNNTTAGCTTANNNNNNNNNNNNNNNNNNNNNNNNTATAAGTGCATGGAAGGAGTGTGTGTATGTGACTGTGTGCTGAATATGTAAGCGTGNGGCGGTAAANNNNNNNNNNNNNNNNNNNNNNTAANTGNNNGACNNAAGCCNCAANGNGCGCTNTGTGCACTGTGTCNATNCGCCCGNGTCANCACNGCCACNCANNCANAACANGCCNNNAGCGNCGTGCATGCTGANCCGNCCCATATCNGNGNNNACCTANNNNACAACNNCANNNNCCGAAAGCNNNCGCCATCCCCNTANACANGNCCCCNNCNGCCACTNCNNCANCNNNGANNCACNTCTGANCCNNGCNCATNTCNNANNNANGACCNCTNNNTCCCGNC
SEQUENCE_INCLUDED_REGION=22,483
SEQUENCE_TARGET=43,14
SEQUENCE_TARGET=62,16
SEQUENCE_TARGET=105,24
SEQUENCE_TARGET=137,24
SEQUENCE_TARGET=227,22
P3_COMMENT=CL is AAGTGCATGGAATGAGTGTGT
P3_COMMENT=CR is CGCTTACATATTCAGCACACAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.387345
PRIMER_LEFT_0_PENALTY=2.927648
PRIMER_RIGHT_0_PENALTY=3.459697
PRIMER_LEFT_0_SEQUENCE=AAGTGCATGGAATGAGTGTGT
PRIMER_RIGHT_0_SEQUENCE=CGCTTACATATTCAGCACACAG
PRIMER_LEFT_0=81,21
PRIMER_RIGHT_0=214,22
PRIMER_LEFT_0_TM=58.072
PRIMER_RIGHT_0_TM=58.540
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=134
PRIMER_PAIR_0_PRODUCT_TM=65.8
PRIMER_PAIR_1_PENALTY=6.393094
PRIMER_LEFT_1_PENALTY=2.933397
PRIMER_RIGHT_1_PENALTY=3.459697
PRIMER_LEFT_1_SEQUENCE=AAGTGCATGGAATGAGTGTG
PRIMER_RIGHT_1_SEQUENCE=CGCTTACATATTCAGCACACAG
PRIMER_LEFT_1=81,20
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=57.067
PRIMER_RIGHT_1_TM=58.540
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=134
PRIMER_PAIR_1_PRODUCT_TM=65.8
PRIMER_PAIR_2_PENALTY=6.439443
PRIMER_LEFT_2_PENALTY=2.927648
PRIMER_RIGHT_2_PENALTY=3.511795
PRIMER_LEFT_2_SEQUENCE=AAGTGCATGGAATGAGTGTGT
PRIMER_RIGHT_2_SEQUENCE=CGCTTACATATTCAGCACACA
PRIMER_LEFT_2=81,21
PRIMER_RIGHT_2=214,21
PRIMER_LEFT_2_TM=58.072
PRIMER_RIGHT_2_TM=57.488
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=134
PRIMER_PAIR_2_PRODUCT_TM=65.8
PRIMER_PAIR_3_PENALTY=6.439443
PRIMER_LEFT_3_PENALTY=2.927648
PRIMER_RIGHT_3_PENALTY=3.511795
PRIMER_LEFT_3_SEQUENCE=AAGTGCATGGAATGAGTGTGT
PRIMER_RIGHT_3_SEQUENCE=CACGCTTACATATTCAGCACA
PRIMER_LEFT_3=81,21
PRIMER_RIGHT_3=216,21
PRIMER_LEFT_3_TM=58.072
PRIMER_RIGHT_3_TM=57.488
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=136
PRIMER_PAIR_3_PRODUCT_TM=66.0
PRIMER_PAIR_4_PENALTY=6.445192
PRIMER_LEFT_4_PENALTY=2.933397
PRIMER_RIGHT_4_PENALTY=3.511795
PRIMER_LEFT_4_SEQUENCE=AAGTGCATGGAATGAGTGTG
PRIMER_RIGHT_4_SEQUENCE=CGCTTACATATTCAGCACACA
PRIMER_LEFT_4=81,20
PRIMER_RIGHT_4=214,21
PRIMER_LEFT_4_TM=57.067
PRIMER_RIGHT_4_TM=57.488
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=134
PRIMER_PAIR_4_PRODUCT_TM=65.8
=
SEQUENCE_ID=02.RHAP64FA8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCANTGACANAGCCTCTCTTGAAAATGAGGTTGAGGACCTGCCTCAGGAACAGGAGCCAGGGTCANTTGTAGCCTCTACACTCTTTTGCANTGAGGTGCAGGTCATATAGATGCAAGGGGCAAAAGGGGATCGGCACGTGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGACAAANACCCAGATTCATANAAAANACAGTNANGTAGGAACAGGNNCACACATACTTANACANNNGTAAACGTGGAAAAATGGCANATNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGNNNNACACACCACACAAAAGANGTGGTNNNNACATNCNAACATNNACNNNAACNANNGTGNNAANTCACNNANTNNTNCNTNTNNTGGNAANNANNCANGNAAAAGTGTNNACACACTTANANNNAGGCAAATNTGTNNGNA
SEQUENCE_INCLUDED_REGION=12,459
SEQUENCE_TARGET=156,50
SEQUENCE_TARGET=297,26
P3_COMMENT=CL is TAGATGCAAGGGGCAAAAG
P3_COMMENT=CR is TGCCATTTTTCCACGTTTAC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.612869
PRIMER_LEFT_0_PENALTY=2.144984
PRIMER_RIGHT_0_PENALTY=1.467885
PRIMER_LEFT_0_SEQUENCE=TAGATGCAAGGGGCAAAAG
PRIMER_RIGHT_0_SEQUENCE=TGCCATTTTTCCACGTTTAC
PRIMER_LEFT_0=119,19
PRIMER_RIGHT_0=293,20
PRIMER_LEFT_0_TM=58.855
PRIMER_RIGHT_0_TM=58.532
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=175
PRIMER_PAIR_0_PRODUCT_TM=68.2
PRIMER_PAIR_1_PENALTY=3.802447
PRIMER_LEFT_1_PENALTY=2.334562
PRIMER_RIGHT_1_PENALTY=1.467885
PRIMER_LEFT_1_SEQUENCE=TAGATGCAAGGGGCAAAAGG
PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTTCCACGTTTAC
PRIMER_LEFT_1=119,20
PRIMER_RIGHT_1=293,20
PRIMER_LEFT_1_TM=62.335
PRIMER_RIGHT_1_TM=58.532
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=68.2
PRIMER_PAIR_2_PENALTY=4.632377
PRIMER_LEFT_2_PENALTY=3.164492
PRIMER_RIGHT_2_PENALTY=1.467885
PRIMER_LEFT_2_SEQUENCE=AAGGGGCAAAAGGGGATC
PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTTCCACGTTTAC
PRIMER_LEFT_2=126,18
PRIMER_RIGHT_2=293,20
PRIMER_LEFT_2_TM=61.164
PRIMER_RIGHT_2_TM=58.532
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=168
PRIMER_PAIR_2_PRODUCT_TM=67.8
PRIMER_PAIR_3_PENALTY=4.671048
PRIMER_LEFT_3_PENALTY=3.203163
PRIMER_RIGHT_3_PENALTY=1.467885
PRIMER_LEFT_3_SEQUENCE=AGATGCAAGGGGCAAAAG
PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTTCCACGTTTAC
PRIMER_LEFT_3=120,18
PRIMER_RIGHT_3=293,20
PRIMER_LEFT_3_TM=58.797
PRIMER_RIGHT_3_TM=58.532
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=68.2
PRIMER_PAIR_4_PENALTY=4.881041
PRIMER_LEFT_4_PENALTY=3.413156
PRIMER_RIGHT_4_PENALTY=1.467885
PRIMER_LEFT_4_SEQUENCE=AGATGCAAGGGGCAAAAGG
PRIMER_RIGHT_4_SEQUENCE=TGCCATTTTTCCACGTTTAC
PRIMER_LEFT_4=120,19
PRIMER_RIGHT_4=293,20
PRIMER_LEFT_4_TM=62.413
PRIMER_RIGHT_4_TM=58.532
PRIMER_LEFT_4_GC_PERCENT=52.632
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=68.2
=
SEQUENCE_ID=03.RHAP64FA3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCTGGAACTGACAAAGTAACCCCAATGTTTGTTGGTAACGTCTTTAGTTCCATGAAACTATCACAGGCTTCAGAGTGCTGTTCTGCCCTGTTCACACTACAAATTCAATTTGATTCTCTTCCGTCTCTAAAGTTCTAATGTAAACTGTACAGCTGACAAAGCATAAATAAGGCTTTAAATGCTAAGGGTTCGCTGTAACAGGATGTAACTGTAATTTACTCTCAAATATATCTTTTAAATCTCCACGTGTGTCAGAAAGAGACAAAGACATGCATGCTTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGGGAGAGGGNNAGGGNGNAGGAAATTGNNAAAATGAAA
SEQUENCE_INCLUDED_REGION=21,423
SEQUENCE_TARGET=304,34
SEQUENCE_TARGET=346,54
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=04.RHAP64FB7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCTCCACTGCAATCAAAAGATGANCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCTCTTATAAGACTTAGGATATTAAAACAAATGAGAGAAATGGACTCTAGGGTATCCAATAGTAAGTCAACAAGGGTTGGTGAGTTCCTGTCCTGTAACAGACCCAAGAGCCACCCACGTCTAATCTGNAGGGANACAAACAGGGGGGCAATAAGGGAATGGGAGAGAGGAAGANAGTCTCATGTCACGCAGAAACGNTCTCCCCGAGCATCNCAAGTGGAGACCTGGGAACAGATTCAGGTAGATTGTAGGAATACAGGGGANATGCCCTAGGNAGCAGCAGGTAGCTGGAGAGAATCNAAGGCTTCTGGGAGCTGATGTGAGAGCATGCACNGAGAAGNNTCCTGCATCTGTCTGTGTCTNAGTTAGTGGTTTCACNNC
SEQUENCE_INCLUDED_REGION=12,455
SEQUENCE_TARGET=39,44
P3_COMMENT=CL is CCTCCACTGCAATCAAAAGA
P3_COMMENT=CR is CAGGAACTCACCAACCCTTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.688139
PRIMER_LEFT_0_PENALTY=1.152564
PRIMER_RIGHT_0_PENALTY=0.535575
PRIMER_LEFT_0_SEQUENCE=CCTCCACTGCAATCAAAAGA
PRIMER_RIGHT_0_SEQUENCE=CAGGAACTCACCAACCCTTG
PRIMER_LEFT_0=12,20
PRIMER_RIGHT_0=175,20
PRIMER_LEFT_0_TM=58.847
PRIMER_RIGHT_0_TM=60.536
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=164
PRIMER_PAIR_0_PRODUCT_TM=67.5
PRIMER_PAIR_1_PENALTY=2.152262
PRIMER_LEFT_1_PENALTY=1.152564
PRIMER_RIGHT_1_PENALTY=0.999698
PRIMER_LEFT_1_SEQUENCE=CCTCCACTGCAATCAAAAGA
PRIMER_RIGHT_1_SEQUENCE=GACAGGAACTCACCAACCCT
PRIMER_LEFT_1=12,20
PRIMER_RIGHT_1=177,20
PRIMER_LEFT_1_TM=58.847
PRIMER_RIGHT_1_TM=59.000
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=166
PRIMER_PAIR_1_PRODUCT_TM=67.6
PRIMER_PAIR_2_PENALTY=2.152262
PRIMER_LEFT_2_PENALTY=1.152564
PRIMER_RIGHT_2_PENALTY=0.999698
PRIMER_LEFT_2_SEQUENCE=CCTCCACTGCAATCAAAAGA
PRIMER_RIGHT_2_SEQUENCE=AGGACAGGAACTCACCAACC
PRIMER_LEFT_2=12,20
PRIMER_RIGHT_2=179,20
PRIMER_LEFT_2_TM=58.847
PRIMER_RIGHT_2_TM=59.000
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=168
PRIMER_PAIR_2_PRODUCT_TM=67.8
PRIMER_PAIR_3_PENALTY=2.261076
PRIMER_LEFT_3_PENALTY=1.152564
PRIMER_RIGHT_3_PENALTY=1.108512
PRIMER_LEFT_3_SEQUENCE=CCTCCACTGCAATCAAAAGA
PRIMER_RIGHT_3_SEQUENCE=GGAACTCACCAACCCTTGTT
PRIMER_LEFT_3=12,20
PRIMER_RIGHT_3=173,20
PRIMER_LEFT_3_TM=58.847
PRIMER_RIGHT_3_TM=58.891
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=162
PRIMER_PAIR_3_PRODUCT_TM=67.3
PRIMER_PAIR_4_PENALTY=2.266317
PRIMER_LEFT_4_PENALTY=1.152564
PRIMER_RIGHT_4_PENALTY=1.113753
PRIMER_LEFT_4_SEQUENCE=CCTCCACTGCAATCAAAAGA
PRIMER_RIGHT_4_SEQUENCE=AGGAACTCACCAACCCTTGTT
PRIMER_LEFT_4=12,20
PRIMER_RIGHT_4=174,21
PRIMER_LEFT_4_TM=58.847
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=163
PRIMER_PAIR_4_PRODUCT_TM=67.3
=
SEQUENCE_ID=06.RHAP64FB3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCTTTAAATGATCAAGGAGTATCAAAANTTCCTCATTTGTGAATANANCTACGTGTATGNGTGTGNNAANGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATCCGTACATGGATCAGAGAAATATAATCCTAAAAATCACAAAAATGACATAAAATGACATCTGTTATTTAAAAAAATCACCTCAGTTCAATGAGAACTAAAATATTTAAATCAACTACAGGAAAAAAAAACACCCCAGCGATTACACACACTGATTCAAAGATTGTGGCACCATTGACATATATTTCATTTTTGNTATAAAGTTTAANGTTTTGTAGGGNTGACTTGAACCCTGAAGAAGGTACCCAGGGTGCAGGTCAGTGCTGCATATGTTATTTACCAGTGCTAGACACTCAAACTTCAGGGTNNNCAC
SEQUENCE_INCLUDED_REGION=21,421
SEQUENCE_TARGET=97,28
P3_COMMENT=CL is CCTTTAAATGATCAAGGAGTATCAA
P3_COMMENT=CR is TTTCTCTGATCCATGTACGGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=9.945457
PRIMER_LEFT_0_PENALTY=7.104630
PRIMER_RIGHT_0_PENALTY=2.840827
PRIMER_LEFT_0_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
PRIMER_RIGHT_0_SEQUENCE=TTTCTCTGATCCATGTACGGA
PRIMER_LEFT_0=21,25
PRIMER_RIGHT_0=150,21
PRIMER_LEFT_0_TM=57.895
PRIMER_RIGHT_0_TM=58.159
PRIMER_LEFT_0_GC_PERCENT=32.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=130
PRIMER_PAIR_0_PRODUCT_TM=66.0
PRIMER_PAIR_1_PENALTY=10.575087
PRIMER_LEFT_1_PENALTY=7.104630
PRIMER_RIGHT_1_PENALTY=3.470456
PRIMER_LEFT_1_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
PRIMER_RIGHT_1_SEQUENCE=TTTCTCTGATCCATGTACGGAT
PRIMER_LEFT_1=21,25
PRIMER_RIGHT_1=150,22
PRIMER_LEFT_1_TM=57.895
PRIMER_RIGHT_1_TM=58.530
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=130
PRIMER_PAIR_1_PRODUCT_TM=66.0
PRIMER_PAIR_2_PENALTY=10.575087
PRIMER_LEFT_2_PENALTY=7.104630
PRIMER_RIGHT_2_PENALTY=3.470456
PRIMER_LEFT_2_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
PRIMER_RIGHT_2_SEQUENCE=ATTTCTCTGATCCATGTACGGA
PRIMER_LEFT_2=21,25
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=57.895
PRIMER_RIGHT_2_TM=58.530
PRIMER_LEFT_2_GC_PERCENT=32.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=131
PRIMER_PAIR_2_PRODUCT_TM=66.0
PRIMER_PAIR_3_PENALTY=11.044168
PRIMER_LEFT_3_PENALTY=7.104630
PRIMER_RIGHT_3_PENALTY=3.939538
PRIMER_LEFT_3_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
PRIMER_RIGHT_3_SEQUENCE=TTCTCTGATCCATGTACGGAT
PRIMER_LEFT_3=21,25
PRIMER_RIGHT_3=149,21
PRIMER_LEFT_3_TM=57.895
PRIMER_RIGHT_3_TM=57.060
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=129
PRIMER_PAIR_3_PRODUCT_TM=66.1
PRIMER_PAIR_4_PENALTY=11.242315
PRIMER_LEFT_4_PENALTY=7.104630
PRIMER_RIGHT_4_PENALTY=4.137685
PRIMER_LEFT_4_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
PRIMER_RIGHT_4_SEQUENCE=ATTTCTCTGATCCATGTACGGAT
PRIMER_LEFT_4=21,25
PRIMER_RIGHT_4=151,23
PRIMER_LEFT_4_TM=57.895
PRIMER_RIGHT_4_TM=58.862
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=131
PRIMER_PAIR_4_PRODUCT_TM=66.0
=
SEQUENCE_ID=07.RHAP64FC7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCAGCCAAANATCGTCAACAAACGCCTGTGACNACCCCCCAAGATCAAAATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGTCAGTGTCTCTCCCNGCACCCATGGACATATGCATGTGCCGACCCAATANGCGTCCATNTATGCATATCCATGTGCATCTGNNCATGTATGTGCACATGTGTGTCTCTCAGTGTGGGTGNACAGGNGTANGGGACATGTGTGCACATGGGCGCANTCCAAATAATCNGGAACTCTTCCCCAGCTTCACCTTGAAGGCATTCCCAGGCGCAAGCAANAGCTANCGTAACCACNGGCAGCATTCTCTNCTCNCCTCCGACCCCTNTAGCTCTCGCCNCATCAGGCCGTAGGCTGNCCCAGCTTCAAACTGNTAAGAGCNTGTCACCCTCCANNCAGAGGNACCCGTGNGNGTGNGGGTCNGC
SEQUENCE_INCLUDED_REGION=12,448
SEQUENCE_TARGET=67,29
P3_COMMENT=CL is CCCCCAAGATCAAAATCCAC
P3_COMMENT=CR is ACCCACACTGAGAGACACACA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.260169
PRIMER_LEFT_0_PENALTY=1.441475
PRIMER_RIGHT_0_PENALTY=1.818694
PRIMER_LEFT_0_SEQUENCE=CCCCCAAGATCAAAATCCAC
PRIMER_RIGHT_0_SEQUENCE=ACCCACACTGAGAGACACACA
PRIMER_LEFT_0=47,20
PRIMER_RIGHT_0=217,21
PRIMER_LEFT_0_TM=61.441
PRIMER_RIGHT_0_TM=59.181
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=73.2
PRIMER_PAIR_1_PENALTY=3.260169
PRIMER_LEFT_1_PENALTY=1.441475
PRIMER_RIGHT_1_PENALTY=1.818694
PRIMER_LEFT_1_SEQUENCE=CCCCCAAGATCAAAATCCAC
PRIMER_RIGHT_1_SEQUENCE=CACCCACACTGAGAGACACAC
PRIMER_LEFT_1=47,20
PRIMER_RIGHT_1=218,21
PRIMER_LEFT_1_TM=61.441
PRIMER_RIGHT_1_TM=59.181
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=172
PRIMER_PAIR_1_PRODUCT_TM=73.3
PRIMER_PAIR_2_PENALTY=3.287638
PRIMER_LEFT_2_PENALTY=1.441475
PRIMER_RIGHT_2_PENALTY=1.846163
PRIMER_LEFT_2_SEQUENCE=CCCCCAAGATCAAAATCCAC
PRIMER_RIGHT_2_SEQUENCE=CCCACACTGAGAGACACACA
PRIMER_LEFT_2=47,20
PRIMER_RIGHT_2=216,20
PRIMER_LEFT_2_TM=61.441
PRIMER_RIGHT_2_TM=58.154
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=170
PRIMER_PAIR_2_PRODUCT_TM=73.3
PRIMER_PAIR_3_PENALTY=3.287638
PRIMER_LEFT_3_PENALTY=1.441475
PRIMER_RIGHT_3_PENALTY=1.846163
PRIMER_LEFT_3_SEQUENCE=CCCCCAAGATCAAAATCCAC
PRIMER_RIGHT_3_SEQUENCE=CACCCACACTGAGAGACACA
PRIMER_LEFT_3=47,20
PRIMER_RIGHT_3=218,20
PRIMER_LEFT_3_TM=61.441
PRIMER_RIGHT_3_TM=58.154
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=172
PRIMER_PAIR_3_PRODUCT_TM=73.3
PRIMER_PAIR_4_PENALTY=3.462692
PRIMER_LEFT_4_PENALTY=1.643998
PRIMER_RIGHT_4_PENALTY=1.818694
PRIMER_LEFT_4_SEQUENCE=CCCCCAAGATCAAAATCCA
PRIMER_RIGHT_4_SEQUENCE=ACCCACACTGAGAGACACACA
PRIMER_LEFT_4=47,19
PRIMER_RIGHT_4=217,21
PRIMER_LEFT_4_TM=60.644
PRIMER_RIGHT_4_TM=59.181
PRIMER_LEFT_4_GC_PERCENT=47.368
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=171
PRIMER_PAIR_4_PRODUCT_TM=73.2
=
SEQUENCE_ID=09.RHAP64FC3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTGACATTCTCTTCCGACCTCTGCAGCCATCAGCATATGTGGTGCACAGACATATGTGTAGGCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACAATTGTTTTTAAGGAAAAAAGTAAAAACAAACAGGAAGAAGAAATGTCTGGTCTACCTTAGGGCCCAGGTTTTAGTGTAACAAAGGCTTGTCTCCTCCCAGGACTTACCATGTTCCCCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,239
SEQUENCE_TARGET=89,45
P3_COMMENT=CL is CTGCAGCCATCAGCATATGT
P3_COMMENT=CR is AACCTGGGCCCTAAGGTAGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.191097
PRIMER_LEFT_0_PENALTY=0.146193
PRIMER_RIGHT_0_PENALTY=0.044904
PRIMER_LEFT_0_SEQUENCE=CTGCAGCCATCAGCATATGT
PRIMER_RIGHT_0_SEQUENCE=AACCTGGGCCCTAAGGTAGA
PRIMER_LEFT_0=41,20
PRIMER_RIGHT_0=208,20
PRIMER_LEFT_0_TM=59.854
PRIMER_RIGHT_0_TM=59.955
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=5.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=168
PRIMER_PAIR_0_PRODUCT_TM=68.0
PRIMER_PAIR_1_PENALTY=0.192939
PRIMER_LEFT_1_PENALTY=0.146193
PRIMER_RIGHT_1_PENALTY=0.046746
PRIMER_LEFT_1_SEQUENCE=CTGCAGCCATCAGCATATGT
PRIMER_RIGHT_1_SEQUENCE=CTAAAACCTGGGCCCTAAGG
PRIMER_LEFT_1=41,20
PRIMER_RIGHT_1=212,20
PRIMER_LEFT_1_TM=59.854
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=172
PRIMER_PAIR_1_PRODUCT_TM=68.1
PRIMER_PAIR_2_PENALTY=0.316731
PRIMER_LEFT_2_PENALTY=0.146193
PRIMER_RIGHT_2_PENALTY=0.170538
PRIMER_LEFT_2_SEQUENCE=CTGCAGCCATCAGCATATGT
PRIMER_RIGHT_2_SEQUENCE=AAAACCTGGGCCCTAAGGTA
PRIMER_LEFT_2=41,20
PRIMER_RIGHT_2=210,20
PRIMER_LEFT_2_TM=59.854
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=170
PRIMER_PAIR_2_PRODUCT_TM=67.9
PRIMER_PAIR_3_PENALTY=0.316731
PRIMER_LEFT_3_PENALTY=0.146193
PRIMER_RIGHT_3_PENALTY=0.170538
PRIMER_LEFT_3_SEQUENCE=CTGCAGCCATCAGCATATGT
PRIMER_RIGHT_3_SEQUENCE=TAAAACCTGGGCCCTAAGGT
PRIMER_LEFT_3=41,20
PRIMER_RIGHT_3=211,20
PRIMER_LEFT_3_TM=59.854
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=171
PRIMER_PAIR_3_PRODUCT_TM=67.9
PRIMER_PAIR_4_PENALTY=0.492707
PRIMER_LEFT_4_PENALTY=0.447804
PRIMER_RIGHT_4_PENALTY=0.044904
PRIMER_LEFT_4_SEQUENCE=CAGCCATCAGCATATGTGGT
PRIMER_RIGHT_4_SEQUENCE=AACCTGGGCCCTAAGGTAGA
PRIMER_LEFT_4=44,20
PRIMER_RIGHT_4=208,20
PRIMER_LEFT_4_TM=59.552
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=165
PRIMER_PAIR_4_PRODUCT_TM=67.7
=
SEQUENCE_ID=11.RHAP64FD8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTTAATTAATGCTTACTGACGGAAAAAAGGACAGGGACAATAATCACCTACTGAACAAAGAAAGTGATATGTATGGAAAANTAAAAGCAGATTATTTCACATAATGTAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATGCTTGCAGCGGGTGCCTATCTTAATCCCAGCAGGCAGACTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=23,193
SEQUENCE_TARGET=135,30
P3_COMMENT=CL is CTGACGGAAAAAAGGACAGG
P3_COMMENT=CR is GTCTGCCTGCTGGGATTAAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.458353
PRIMER_LEFT_0_PENALTY=0.294694
PRIMER_RIGHT_0_PENALTY=0.163658
PRIMER_LEFT_0_SEQUENCE=CTGACGGAAAAAAGGACAGG
PRIMER_RIGHT_0_SEQUENCE=GTCTGCCTGCTGGGATTAAG
PRIMER_LEFT_0=38,20
PRIMER_RIGHT_0=210,20
PRIMER_LEFT_0_TM=59.705
PRIMER_RIGHT_0_TM=59.836
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=173
PRIMER_PAIR_0_PRODUCT_TM=69.7
PRIMER_PAIR_1_PENALTY=0.458353
PRIMER_LEFT_1_PENALTY=0.294694
PRIMER_RIGHT_1_PENALTY=0.163658
PRIMER_LEFT_1_SEQUENCE=CTGACGGAAAAAAGGACAGG
PRIMER_RIGHT_1_SEQUENCE=AGTCTGCCTGCTGGGATTAA
PRIMER_LEFT_1=38,20
PRIMER_RIGHT_1=211,20
PRIMER_LEFT_1_TM=59.705
PRIMER_RIGHT_1_TM=59.836
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=174
PRIMER_PAIR_1_PRODUCT_TM=69.7
PRIMER_PAIR_2_PENALTY=0.504116
PRIMER_LEFT_2_PENALTY=0.340458
PRIMER_RIGHT_2_PENALTY=0.163658
PRIMER_LEFT_2_SEQUENCE=ACGGAAAAAAGGACAGGGAC
PRIMER_RIGHT_2_SEQUENCE=GTCTGCCTGCTGGGATTAAG
PRIMER_LEFT_2=41,20
PRIMER_RIGHT_2=210,20
PRIMER_LEFT_2_TM=60.340
PRIMER_RIGHT_2_TM=59.836
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=170
PRIMER_PAIR_2_PRODUCT_TM=69.4
PRIMER_PAIR_3_PENALTY=0.504116
PRIMER_LEFT_3_PENALTY=0.340458
PRIMER_RIGHT_3_PENALTY=0.163658
PRIMER_LEFT_3_SEQUENCE=ACGGAAAAAAGGACAGGGAC
PRIMER_RIGHT_3_SEQUENCE=AGTCTGCCTGCTGGGATTAA
PRIMER_LEFT_3=41,20
PRIMER_RIGHT_3=211,20
PRIMER_LEFT_3_TM=60.340
PRIMER_RIGHT_3_TM=59.836
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=171
PRIMER_PAIR_3_PRODUCT_TM=69.3
PRIMER_PAIR_4_PENALTY=0.947784
PRIMER_LEFT_4_PENALTY=0.294694
PRIMER_RIGHT_4_PENALTY=0.653090
PRIMER_LEFT_4_SEQUENCE=CTGACGGAAAAAAGGACAGG
PRIMER_RIGHT_4_SEQUENCE=GGGATTAAGATAGGCACCCG
PRIMER_LEFT_4=38,20
PRIMER_RIGHT_4=199,20
PRIMER_LEFT_4_TM=59.705
PRIMER_RIGHT_4_TM=60.653
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=162
PRIMER_PAIR_4_PRODUCT_TM=68.6
=
SEQUENCE_ID=12.RHAP64FD9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCACTATATCCCCTGCCTTTGTGGACCTGAGCATCTCATTGACGAATCCCAAATTATACCCCCTTCATTATTCATTAAAGTGTGGTGGGCAGAAATGTGTGTTCACGAATTCAGCTATGGAGAGGTTATAACTGAAGCCTCTGAATGCCCCACAGGGACCAATGTCAGCCAGTTCTATGCCCCTGTTCAGTATCTGCTTGAATCTGAAGCCGGACTCTGTATGTATGTGTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCANATATATATANNTTNTCNNNGGGGGGGGGGGGGNGAGGGAGAANANNNNNNCGGAGGNNNTANNNTGNGCNAGGTGNNNNNNTATNNGNNNACNCNCNTCNNGGGGC
SEQUENCE_INCLUDED_REGION=13,445
SEQUENCE_TARGET=246,44
SEQUENCE_TARGET=302,46
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=14.RHAP64FE2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
SEQUENCE_INCLUDED_REGION=19,432
SEQUENCE_TARGET=44,46
SEQUENCE_TARGET=92,33
SEQUENCE_TARGET=140,26
SEQUENCE_TARGET=170,14
SEQUENCE_TARGET=211,20
SEQUENCE_TARGET=238,26
SEQUENCE_TARGET=336,14
P3_COMMENT=CL is CACATGTGCACAGTGGTATGT
P3_COMMENT=CR is GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.458561
PRIMER_LEFT_0_PENALTY=1.104149
PRIMER_RIGHT_0_PENALTY=5.354412
PRIMER_LEFT_0_SEQUENCE=TCCACATGTGCACAGTGGTAT
PRIMER_RIGHT_0_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_0=21,21
PRIMER_RIGHT_0=313,23
PRIMER_LEFT_0_TM=59.896
PRIMER_RIGHT_0_TM=57.646
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=293
PRIMER_PAIR_0_PRODUCT_TM=64.3
PRIMER_PAIR_1_PENALTY=8.467518
PRIMER_LEFT_1_PENALTY=3.113106
PRIMER_RIGHT_1_PENALTY=5.354412
PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT
PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_1=23,21
PRIMER_RIGHT_1=313,23
PRIMER_LEFT_1_TM=57.887
PRIMER_RIGHT_1_TM=57.646
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=291
PRIMER_PAIR_1_PRODUCT_TM=64.2
PRIMER_PAIR_2_PENALTY=8.789928
PRIMER_LEFT_2_PENALTY=1.104149
PRIMER_RIGHT_2_PENALTY=7.685778
PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT
PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT
PRIMER_LEFT_2=21,21
PRIMER_RIGHT_2=288,25
PRIMER_LEFT_2_TM=59.896
PRIMER_RIGHT_2_TM=57.314
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=24.000
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=268
PRIMER_PAIR_2_PRODUCT_TM=63.2
PRIMER_PAIR_3_PENALTY=9.104196
PRIMER_LEFT_3_PENALTY=3.749784
PRIMER_RIGHT_3_PENALTY=5.354412
PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT
PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=313,23
PRIMER_LEFT_3_TM=61.750
PRIMER_RIGHT_3_TM=57.646
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=292
PRIMER_PAIR_3_PRODUCT_TM=64.3
PRIMER_PAIR_4_PENALTY=10.198358
PRIMER_LEFT_4_PENALTY=4.843946
PRIMER_RIGHT_4_PENALTY=5.354412
PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG
PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=313,23
PRIMER_LEFT_4_TM=62.844
PRIMER_RIGHT_4_TM=57.646
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=293
PRIMER_PAIR_4_PRODUCT_TM=64.3
=
SEQUENCE_ID=15.RHAP64FE3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCGACGACCTGACTTGGATCTTCTGGAACCACCTGGTAGAAGGGAGAATATGGTTGTCTTCAGACCACTGCAAGCCGTAGTAAATGGATGTAAATATATGTGCACACACAGAGTAATTATAAGTAATGAAAGAAAAGAAGTAAACACTTTCCATAATGTAAAGAAGAAGGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTATTCTTCACACAGTGACCCTCTCCACAGAGTCTCTCCTGTGTATTCCGCACACAGTGATCCTCTCCACCACCACCAATGACTTTTCTATAGCACGAATCTAATTCCGCTGGTAAAAGTCCTGGGTTGGCATGCCACAATTTTTCTTGTTCTTTTTAAAAGTAATTTTAGAGTAAATTTACAAAGTAATTNGTATTT
SEQUENCE_INCLUDED_REGION=20,420
SEQUENCE_TARGET=194,45
P3_COMMENT=CL is TCCATAATGTAAAGAAGAAGGCAA
P3_COMMENT=CR is TGGAGAGGATCACTGTGTGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.960761
PRIMER_LEFT_0_PENALTY=4.787991
PRIMER_RIGHT_0_PENALTY=0.172769
PRIMER_LEFT_0_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
PRIMER_RIGHT_0_SEQUENCE=TGGAGAGGATCACTGTGTGC
PRIMER_LEFT_0=168,24
PRIMER_RIGHT_0=310,20
PRIMER_LEFT_0_TM=59.212
PRIMER_RIGHT_0_TM=59.827
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=143
PRIMER_PAIR_0_PRODUCT_TM=68.3
PRIMER_PAIR_1_PENALTY=4.960761
PRIMER_LEFT_1_PENALTY=4.787991
PRIMER_RIGHT_1_PENALTY=0.172769
PRIMER_LEFT_1_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA
PRIMER_RIGHT_1_SEQUENCE=TGGAGAGGATCACTGTGTGC
PRIMER_LEFT_1=167,24
PRIMER_RIGHT_1=310,20
PRIMER_LEFT_1_TM=59.212
PRIMER_RIGHT_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=144
PRIMER_PAIR_1_PRODUCT_TM=68.3
PRIMER_PAIR_2_PENALTY=4.963076
PRIMER_LEFT_2_PENALTY=4.787991
PRIMER_RIGHT_2_PENALTY=0.175084
PRIMER_LEFT_2_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
PRIMER_RIGHT_2_SEQUENCE=CTGTGTGCGGAATACACAGG
PRIMER_LEFT_2=168,24
PRIMER_RIGHT_2=298,20
PRIMER_LEFT_2_TM=59.212
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=131
PRIMER_PAIR_2_PRODUCT_TM=67.2
PRIMER_PAIR_3_PENALTY=4.963076
PRIMER_LEFT_3_PENALTY=4.787991
PRIMER_RIGHT_3_PENALTY=0.175084
PRIMER_LEFT_3_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA
PRIMER_RIGHT_3_SEQUENCE=CTGTGTGCGGAATACACAGG
PRIMER_LEFT_3=167,24
PRIMER_RIGHT_3=298,20
PRIMER_LEFT_3_TM=59.212
PRIMER_RIGHT_3_TM=60.175
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=132
PRIMER_PAIR_3_PRODUCT_TM=67.2
PRIMER_PAIR_4_PENALTY=5.051337
PRIMER_LEFT_4_PENALTY=4.787991
PRIMER_RIGHT_4_PENALTY=0.263346
PRIMER_LEFT_4_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
PRIMER_RIGHT_4_SEQUENCE=TGTGCGGAATACACAGGAGA
PRIMER_LEFT_4=168,24
PRIMER_RIGHT_4=295,20
PRIMER_LEFT_4_TM=59.212
PRIMER_RIGHT_4_TM=60.263
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=128
PRIMER_PAIR_4_PRODUCT_TM=66.7
=
SEQUENCE_ID=15.RHAP64FE9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCTGAGCACCAGAAACCACAGAGTTGTGCTATACNNNNTGAGCAATAGCCCAAGCTGCGTGTTAGCTCTGCACAGCATGGTGCTAACTGTGTTGTTTTGTCAATCCCTCACCACTANCTGTGNGTGTGCANNNNNNNNNNNNNNNNNNNNNNNGTGNNCANGNGGGTGAANGTGTATGNGTGNGCNTGCGNNNNNNNNNNNNNNNNNNGCATGNGNNNNNNNNNNNNNNNNNNNTATGNGNATGNGGCGNGAGNGTGTGNATGTGTGGTNTGCATGTATGTGNNNGTNCAATGTGGTANNNTNGTGNGGCANNCNTGNNNGTGTGNTGNTGGNNGTGNTAANNGGTGNAGNGTGNGNGCGGGCGNGNGNTGGTNTNGTGTGNGGTTGNGGNNNNGNGTGTNGNGGNNCNCNNGGANNGCGNNNNNNNNNCCGNGCNGGNTCNNGGNANTANTNGNNNANNGNNNGNANAGNNGAC
SEQUENCE_INCLUDED_REGION=13,475
SEQUENCE_TARGET=143,23
SEQUENCE_TARGET=204,15
SEQUENCE_TARGET=229,18
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=16.RHAP64FF7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCATTCTTTGCTATTTATTGTTCCTGGGTAGCATTTCCTAAATACATCCATGAAGTGCTTTTTCTGTAGAAGGTTAACTTGTGTAACATGTGGCAGGACCTAGTACTGAGTGAGTTTGCCTTCACTTCAGTCTCACAAGAACTTGAATAGGCTGGTGTCTAATAATGGAGCTGTTGAAATGTTGGGNTCCTGAACTTTTCAGATTTGTCAATGGACTAGGGTCATGTGAAACACACATTGAAGAGAACTTTCAGTTGGACTNTTNAAGGGTANCCTTTTNNTTTTTAGAGATCTTNATNATTATTTTTTGCCACGNGTACNNNNNNNNNNNNNNNNNACTANGTGCATGTAAGTACAGACGCCTGTGGAAGGTGGAGANTTGGATCCCCTGGAGCTGGAGTTAAAGGCGGTTATAAGTTAGGAACTGAACTGAGGTCCT
SEQUENCE_INCLUDED_REGION=23,438
SEQUENCE_TARGET=343,16
P3_COMMENT=CL is TTGTCAATGGACTAGGGTCATG
P3_COMMENT=CR is ACCTTCCACAGGCGTCTGTA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.858479
PRIMER_LEFT_0_PENALTY=2.147295
PRIMER_RIGHT_0_PENALTY=0.711184
PRIMER_LEFT_0_SEQUENCE=CAATGGACTAGGGTCATGTGAA
PRIMER_RIGHT_0_SEQUENCE=CCTTCCACAGGCGTCTGTAC
PRIMER_LEFT_0=230,22
PRIMER_RIGHT_0=393,20
PRIMER_LEFT_0_TM=59.853
PRIMER_RIGHT_0_TM=60.711
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=164
PRIMER_PAIR_0_PRODUCT_TM=69.7
PRIMER_PAIR_1_PENALTY=2.858479
PRIMER_LEFT_1_PENALTY=2.147295
PRIMER_RIGHT_1_PENALTY=0.711184
PRIMER_LEFT_1_SEQUENCE=CAATGGACTAGGGTCATGTGAA
PRIMER_RIGHT_1_SEQUENCE=ACCTTCCACAGGCGTCTGTA
PRIMER_LEFT_1=230,22
PRIMER_RIGHT_1=394,20
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=60.711
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=69.7
PRIMER_PAIR_2_PENALTY=2.858479
PRIMER_LEFT_2_PENALTY=2.147295
PRIMER_RIGHT_2_PENALTY=0.711184
PRIMER_LEFT_2_SEQUENCE=TTGTCAATGGACTAGGGTCATG
PRIMER_RIGHT_2_SEQUENCE=CCTTCCACAGGCGTCTGTAC
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=393,20
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.711
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=168
PRIMER_PAIR_2_PRODUCT_TM=69.8
PRIMER_PAIR_3_PENALTY=2.858479
PRIMER_LEFT_3_PENALTY=2.147295
PRIMER_RIGHT_3_PENALTY=0.711184
PRIMER_LEFT_3_SEQUENCE=TTGTCAATGGACTAGGGTCATG
PRIMER_RIGHT_3_SEQUENCE=ACCTTCCACAGGCGTCTGTA
PRIMER_LEFT_3=226,22
PRIMER_RIGHT_3=394,20
PRIMER_LEFT_3_TM=59.853
PRIMER_RIGHT_3_TM=60.711
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=169
PRIMER_PAIR_3_PRODUCT_TM=69.7
PRIMER_PAIR_4_PENALTY=3.086274
PRIMER_LEFT_4_PENALTY=2.375090
PRIMER_RIGHT_4_PENALTY=0.711184
PRIMER_LEFT_4_SEQUENCE=TCAATGGACTAGGGTCATGTGA
PRIMER_RIGHT_4_SEQUENCE=CCTTCCACAGGCGTCTGTAC
PRIMER_LEFT_4=229,22
PRIMER_RIGHT_4=393,20
PRIMER_LEFT_4_TM=60.375
PRIMER_RIGHT_4_TM=60.711
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=165
PRIMER_PAIR_4_PRODUCT_TM=69.7
=
SEQUENCE_ID=17.RHAP64FF8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCCCAGTCCAAGTTTGCAGTAGTTCCCTAGTTAGGGAATATTTTTCTGATGACATAGGTGTAGTCTCATCACTGTGTGCTTACTGCCTAGATTAGCACAGAGCAAAGAATGAATTGGTTTTCCAGGGAAAATTGCTAAATTAGTAAACACGAGAGTAAGGCAGGAAAGAGCAACAATTTTACTCACAGGTTAATGGTTCACGTTTAGTCAAATTCCAAACTATGGCTCGGTGGTTGGTATAATTATAAGGACATGTTCTATGATTCCATCTCTTTATTAAATATCTCTAATCNAGGGACTGCNAGGAAATGGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCACTCACGGTGTGCATGTGGANNNNNNNNNNNNNNNNNNNNNNGTGTCTGAAATATNTCTTGTGTGTGCNAGGNG
SEQUENCE_INCLUDED_REGION=23,443
SEQUENCE_TARGET=340,48
SEQUENCE_TARGET=412,22
P3_COMMENT=CL is AAACTATGGCTCGGTGGTTG
P3_COMMENT=CR is CACATGCACACCGTGAGTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.849917
PRIMER_LEFT_0_PENALTY=0.006945
PRIMER_RIGHT_0_PENALTY=1.842973
PRIMER_LEFT_0_SEQUENCE=AAACTATGGCTCGGTGGTTG
PRIMER_RIGHT_0_SEQUENCE=CACATGCACACCGTGAGTG
PRIMER_LEFT_0=239,20
PRIMER_RIGHT_0=409,19
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.843
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=68.4
PRIMER_PAIR_1_PENALTY=1.849917
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=1.842973
PRIMER_LEFT_1_SEQUENCE=CAAACTATGGCTCGGTGGTT
PRIMER_RIGHT_1_SEQUENCE=CACATGCACACCGTGAGTG
PRIMER_LEFT_1=238,20
PRIMER_RIGHT_1=409,19
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.843
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=172
PRIMER_PAIR_1_PRODUCT_TM=68.6
PRIMER_PAIR_2_PENALTY=2.056478
PRIMER_LEFT_2_PENALTY=0.213506
PRIMER_RIGHT_2_PENALTY=1.842973
PRIMER_LEFT_2_SEQUENCE=ATGGCTCGGTGGTTGGTATA
PRIMER_RIGHT_2_SEQUENCE=CACATGCACACCGTGAGTG
PRIMER_LEFT_2=244,20
PRIMER_RIGHT_2=409,19
PRIMER_LEFT_2_TM=60.214
PRIMER_RIGHT_2_TM=60.843
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=4.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=68.4
PRIMER_PAIR_3_PENALTY=2.056478
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=1.842973
PRIMER_LEFT_3_SEQUENCE=TATGGCTCGGTGGTTGGTAT
PRIMER_RIGHT_3_SEQUENCE=CACATGCACACCGTGAGTG
PRIMER_LEFT_3=243,20
PRIMER_RIGHT_3=409,19
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=60.843
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.8000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=68.3
PRIMER_PAIR_4_PENALTY=2.721606
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=2.714661
PRIMER_LEFT_4_SEQUENCE=AAACTATGGCTCGGTGGTTG
PRIMER_RIGHT_4_SEQUENCE=CCACATGCACACCGTGAGT
PRIMER_LEFT_4=239,20
PRIMER_RIGHT_4=410,19
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=61.715
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=68.6
=
SEQUENCE_ID=18.RHAP64FF9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCTTGGAAAATCACTGGGAAAGGGCTAAGTTGGCACGCCCCTTAGAGCAGGGAGGAACTNGGTGGTCGGCAGGGTCATTATGACATCTTGTGGTTGTTCTCAAGATTTACCACAGGGAGGGGTAGGGGGGTCTTTCCGAGAACAGTATCTTTGTTTTGTACTAACTTATGGTCAGAATAGTCTGGTCATTCTGACTACTANAACTTTGTCATAAATTAGGCAACCGGAAGTCAGATGTATTTCTCAAAACCTTGTTTTTACAACTTTGTTTCTTTGNNTCNCATATCTATGGAACTATGAAACCTTANTTCNTCAGNGGGGGGGGGGGGGAGAAAGACNNNNNNNNNNNNNNNNNNGAGNGGAGAGAGGANAGNGGANAGACNGANNNAGNNANGANAGACANAGACAGAGNACNGGAAAGAGNNNNNCAAGNNNCAGAAGGNGNGNNNNG
SEQUENCE_INCLUDED_REGION=13,451
SEQUENCE_TARGET=351,18
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=19.RHAP64FG1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGAGAGTGATCTACCTACTGTTGGTTCCAGAGCCTGAGTCTGTTACAGCGGTCTGCACACACCACCATTCCCATTTCCAAGATAAGGAAANTGGGTCAAGGTNTTTGGGTGAGCGTGCGACAGATGAACTCATGGTCAAGGACTTCTTGGCTTTGAAAACCTGACCCTCACTCCCAAGTGTTCTCTGGGCTGGTTTGCCTCAGTTGGTTTTAACAGACTGGTCTAAAATTTCACCTCAAAATATAGAGCATGAAAGTAGAAATAAAGCTTCAATATCTGTCCAAATCTCATTTGTTCTATTTTTTATCATCATCATCATCACCACCACCACCATCATCATCATTACCACCACCATCATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACNATCACCANCACCATCATCACCACC
SEQUENCE_INCLUDED_REGION=18,427
SEQUENCE_TARGET=381,35
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=21.RHAP64FG3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCAGCCAATCTAACCAGTTAACATGCTCCAGGTTCAGTGAGAGACTTTCAAAAGGAATAAAGCCATAGAGAGGGGCATCCAGTGTTGACCTATGGCATGNNNNNNNNNNNNNNGGCATGTGCACCCATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGCACTTCATTAGTCAAGTACAGCAGAGTGAACAGGCTAGAAGGTAACAGGTGACCACGTGTACCTCCCTCCACCCCATCCTCCTACCCCCACACACCCTCTGCAGGGAGCTGCCTGGAGAAACAGNAGAGCCTGTGGTAAGGTGTGCTGGGAGGGTGGAGAGTTAAGGAAAAGTGCAGAAAGCGATCTGTGTGGTTTCCACAGAAAGGNATGGCTGCCGTGGAATGTGTCCGGG
SEQUENCE_INCLUDED_REGION=21,423
SEQUENCE_TARGET=120,14
SEQUENCE_TARGET=152,55
P3_COMMENT=CL is TCCAGTGTTGACCTATGGCA
P3_COMMENT=CR is CACGTGGTCACCTGTTACCTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.173834
PRIMER_LEFT_0_PENALTY=0.112124
PRIMER_RIGHT_0_PENALTY=1.061709
PRIMER_LEFT_0_SEQUENCE=TCCAGTGTTGACCTATGGCA
PRIMER_RIGHT_0_SEQUENCE=CACGTGGTCACCTGTTACCTT
PRIMER_LEFT_0=98,20
PRIMER_RIGHT_0=269,21
PRIMER_LEFT_0_TM=60.112
PRIMER_RIGHT_0_TM=59.938
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=172
PRIMER_PAIR_0_PRODUCT_TM=69.0
PRIMER_PAIR_1_PENALTY=1.621151
PRIMER_LEFT_1_PENALTY=0.112124
PRIMER_RIGHT_1_PENALTY=1.509026
PRIMER_LEFT_1_SEQUENCE=TCCAGTGTTGACCTATGGCA
PRIMER_RIGHT_1_SEQUENCE=CACGTGGTCACCTGTTACCT
PRIMER_LEFT_1=98,20
PRIMER_RIGHT_1=269,20
PRIMER_LEFT_1_TM=60.112
PRIMER_RIGHT_1_TM=58.491
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=172
PRIMER_PAIR_1_PRODUCT_TM=69.0
PRIMER_PAIR_2_PENALTY=1.702135
PRIMER_LEFT_2_PENALTY=0.112124
PRIMER_RIGHT_2_PENALTY=1.590010
PRIMER_LEFT_2_SEQUENCE=TCCAGTGTTGACCTATGGCA
PRIMER_RIGHT_2_SEQUENCE=ACACGTGGTCACCTGTTACCT
PRIMER_LEFT_2=98,20
PRIMER_RIGHT_2=270,21
PRIMER_LEFT_2_TM=60.112
PRIMER_RIGHT_2_TM=59.410
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=173
PRIMER_PAIR_2_PRODUCT_TM=69.0
PRIMER_PAIR_3_PENALTY=1.779345
PRIMER_LEFT_3_PENALTY=0.112124
PRIMER_RIGHT_3_PENALTY=1.667221
PRIMER_LEFT_3_SEQUENCE=TCCAGTGTTGACCTATGGCA
PRIMER_RIGHT_3_SEQUENCE=ACACGTGGTCACCTGTTACC
PRIMER_LEFT_3=98,20
PRIMER_RIGHT_3=270,20
PRIMER_LEFT_3_TM=60.112
PRIMER_RIGHT_3_TM=58.333
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=173
PRIMER_PAIR_3_PRODUCT_TM=69.0
PRIMER_PAIR_4_PENALTY=1.989876
PRIMER_LEFT_4_PENALTY=0.112124
PRIMER_RIGHT_4_PENALTY=1.877751
PRIMER_LEFT_4_SEQUENCE=TCCAGTGTTGACCTATGGCA
PRIMER_RIGHT_4_SEQUENCE=CGTGGTCACCTGTTACCTTCT
PRIMER_LEFT_4=98,20
PRIMER_RIGHT_4=267,21
PRIMER_LEFT_4_TM=60.112
PRIMER_RIGHT_4_TM=59.122
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=170
PRIMER_PAIR_4_PRODUCT_TM=68.9
=
SEQUENCE_ID=22.RHAP64FH7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCACCCCTTGTTGTCTTTACTATGNGCCTCTATATGACTATACGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNTCNCTCTCNTNNNNTNNANANANANNNNNNNNNNNNNNNNNNNNNTGNGNNGNNNGTGTGNGNGTNNNTCNNTCNNATNCNNNANNGCCANNTGGANTANNNNANNNTCNTNNNAANTAGANNNGGGNNNTNGNANTNNCCNGGAGGTNTCNCANCCACNNCTCNGCANANGNACANNTANNCNNCAGNACTCACATNCTNNGANANACNNNTNTGTTCTGGNNNCCANTNNNAANGNTNNNTTNNNNATAATNNNNNGNNCTCNNCANNGTGNNNNTCNNACTCCNNNNTANGNNTNNNGNTTNCAGNNGNANNNNNAGTANANGNNACNTCNNGNNTNNTGATGGNNNNNNNNNTNTNNGNNNCCNNNTNCNGANNTNNNNNNNNTNG
SEQUENCE_INCLUDED_REGION=12,488
SEQUENCE_TARGET=57,22
SEQUENCE_TARGET=137,18
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=26.RHAP64FA11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCATCCTACTAAGGGAAAGCAAAAAAAAAACTTCCTCCTGACTNTGATGTTGAAAGAAGAATGAAACGAACATCTCTCCAACAATATCTAATCAGCAGGAGTCATACTCACCCTTAGGTCTAGATTTACACTTTTCCAAGATCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGGGGGGGTGGAGAGGAAGAAGGACAGAGACACAGAGAGATCACTAGACATATAATACACTACGAATTGTACAATTAAGAATTCAAAAAATTAAAAAAAAAACCATTTCTGATAGCCAGACAAGTCAGTTTGATGAACAAAGAGGAAGAATGTGAGTGAGGAAGGATGNAATTGGAGAGAGGGATTGAAAATGGCAGTANTGTTAAAGATTGGAGAAGAAGGAAGTCCTGTGGGAGAGGGGGTGGGGGAGA
SEQUENCE_INCLUDED_REGION=12,445
SEQUENCE_TARGET=157,46
P3_COMMENT=CL is CAGCAGGAGTCATACTCACCC
P3_COMMENT=CR is GTCCTTCTTCCTCTCCACCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.314269
PRIMER_LEFT_0_PENALTY=1.263803
PRIMER_RIGHT_0_PENALTY=0.050466
PRIMER_LEFT_0_SEQUENCE=CAGCAGGAGTCATACTCACCC
PRIMER_RIGHT_0_SEQUENCE=GTCCTTCTTCCTCTCCACCC
PRIMER_LEFT_0=103,21
PRIMER_RIGHT_0=230,20
PRIMER_LEFT_0_TM=59.736
PRIMER_RIGHT_0_TM=60.050
PRIMER_LEFT_0_GC_PERCENT=57.143
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=128
PRIMER_PAIR_0_PRODUCT_TM=68.0
PRIMER_PAIR_1_PENALTY=2.035901
PRIMER_LEFT_1_PENALTY=1.263803
PRIMER_RIGHT_1_PENALTY=0.772097
PRIMER_LEFT_1_SEQUENCE=CAGCAGGAGTCATACTCACCC
PRIMER_RIGHT_1_SEQUENCE=TGTCCTTCTTCCTCTCCACC
PRIMER_LEFT_1=103,21
PRIMER_RIGHT_1=231,20
PRIMER_LEFT_1_TM=59.736
PRIMER_RIGHT_1_TM=59.228
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=129
PRIMER_PAIR_1_PRODUCT_TM=68.0
PRIMER_PAIR_2_PENALTY=2.501080
PRIMER_LEFT_2_PENALTY=1.263803
PRIMER_RIGHT_2_PENALTY=1.237277
PRIMER_LEFT_2_SEQUENCE=CAGCAGGAGTCATACTCACCC
PRIMER_RIGHT_2_SEQUENCE=CTGTCCTTCTTCCTCTCCACC
PRIMER_LEFT_2=103,21
PRIMER_RIGHT_2=232,21
PRIMER_LEFT_2_TM=59.736
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=130
PRIMER_PAIR_2_PRODUCT_TM=68.2
PRIMER_PAIR_3_PENALTY=2.718781
PRIMER_LEFT_3_PENALTY=2.668315
PRIMER_RIGHT_3_PENALTY=0.050466
PRIMER_LEFT_3_SEQUENCE=TGAAACGAACATCTCTCCAACA
PRIMER_RIGHT_3_SEQUENCE=GTCCTTCTTCCTCTCCACCC
PRIMER_LEFT_3=72,22
PRIMER_RIGHT_3=230,20
PRIMER_LEFT_3_TM=60.668
PRIMER_RIGHT_3_TM=60.050
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=159
PRIMER_PAIR_3_PRODUCT_TM=69.0
PRIMER_PAIR_4_PENALTY=2.751591
PRIMER_LEFT_4_PENALTY=2.701125
PRIMER_RIGHT_4_PENALTY=0.050466
PRIMER_LEFT_4_SEQUENCE=CAGCAGGAGTCATACTCACCCT
PRIMER_RIGHT_4_SEQUENCE=GTCCTTCTTCCTCTCCACCC
PRIMER_LEFT_4=103,22
PRIMER_RIGHT_4=230,20
PRIMER_LEFT_4_TM=60.701
PRIMER_RIGHT_4_TM=60.050
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=128
PRIMER_PAIR_4_PRODUCT_TM=68.0
=
SEQUENCE_ID=30.RHAP64FB12.seq
SEQUENCE_TEMPLATE=CCTTCCTATTTTCCTCTCCAGTGCTGAGACCAAAGTGAGATCAAAGTCATGCATNACCATGGGNACCTTCCTTTTCANNANTTTNTTTCATCNTAGGAAGTANGTANANANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTTAAAATCAGCACTCAAGANNTNTNNGNTTCCTATCCCTATANCANNCCCCCCAAAACANCCNNAAAGCCACCNGCNCCAGAGAAAGAGGGAGATGAAATCTCTAGTNNGAAAAANNAGNNGAGGGAGATNACAGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNANANACACAGATACANNNCCNNANANNNNGGNGANACNNNNNNNNNGNNGCNNGCGNGCNNNGAGTANTNNANAAANANNGNTNCTNNNNGNGNNNCNNGGNGNNNA
SEQUENCE_INCLUDED_REGION=0,451
SEQUENCE_TARGET=113,32
SEQUENCE_TARGET=146,26
SEQUENCE_TARGET=314,24
P3_COMMENT=CL is TCTCCAGTGCTGAGACCAAA
P3_COMMENT=CR is TTTCATCTCCCTCTTTCTCTGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.634023
PRIMER_LEFT_0_PENALTY=0.454603
PRIMER_RIGHT_0_PENALTY=2.179421
PRIMER_LEFT_0_SEQUENCE=TCTCCAGTGCTGAGACCAAA
PRIMER_RIGHT_0_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
PRIMER_LEFT_0=14,20
PRIMER_RIGHT_0=273,22
PRIMER_LEFT_0_TM=59.545
PRIMER_RIGHT_0_TM=59.821
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=260
PRIMER_PAIR_0_PRODUCT_TM=69.7
PRIMER_PAIR_1_PENALTY=2.718057
PRIMER_LEFT_1_PENALTY=0.538636
PRIMER_RIGHT_1_PENALTY=2.179421
PRIMER_LEFT_1_SEQUENCE=CCAGTGCTGAGACCAAAGTG
PRIMER_RIGHT_1_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
PRIMER_LEFT_1=17,20
PRIMER_RIGHT_1=273,22
PRIMER_LEFT_1_TM=59.461
PRIMER_RIGHT_1_TM=59.821
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=257
PRIMER_PAIR_1_PRODUCT_TM=69.7
PRIMER_PAIR_2_PENALTY=2.729191
PRIMER_LEFT_2_PENALTY=0.549770
PRIMER_RIGHT_2_PENALTY=2.179421
PRIMER_LEFT_2_SEQUENCE=TCCTCTCCAGTGCTGAGACC
PRIMER_RIGHT_2_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
PRIMER_LEFT_2=11,20
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=60.550
PRIMER_RIGHT_2_TM=59.821
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=263
PRIMER_PAIR_2_PRODUCT_TM=69.9
PRIMER_PAIR_3_PENALTY=2.996036
PRIMER_LEFT_3_PENALTY=0.454603
PRIMER_RIGHT_3_PENALTY=2.541433
PRIMER_LEFT_3_SEQUENCE=TCTCCAGTGCTGAGACCAAA
PRIMER_RIGHT_3_SEQUENCE=TTCATCTCCCTCTTTCTCTGG
PRIMER_LEFT_3=14,20
PRIMER_RIGHT_3=272,21
PRIMER_LEFT_3_TM=59.545
PRIMER_RIGHT_3_TM=58.459
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=259
PRIMER_PAIR_3_PRODUCT_TM=69.8
PRIMER_PAIR_4_PENALTY=3.054883
PRIMER_LEFT_4_PENALTY=0.875462
PRIMER_RIGHT_4_PENALTY=2.179421
PRIMER_LEFT_4_SEQUENCE=CTCTCCAGTGCTGAGACCAA
PRIMER_RIGHT_4_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
PRIMER_LEFT_4=13,20
PRIMER_RIGHT_4=273,22
PRIMER_LEFT_4_TM=59.125
PRIMER_RIGHT_4_TM=59.821
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=261
PRIMER_PAIR_4_PRODUCT_TM=69.9
=
SEQUENCE_ID=31.RHAP64FC10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCCTAACCCTTTATGCTATCTAGTTACTTCCCAGACATGCACACACGCACGCACGCACGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCCCCAAGTTACTTGCATTTAGTCTCACCTTGGATACATTCAGCTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=24,167
SEQUENCE_TARGET=86,52
P3_COMMENT=CL is CTTCCCAGACATGCACACAC
P3_COMMENT=CR is CCCACAGCTGAATGTATCCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.640953
PRIMER_LEFT_0_PENALTY=0.162457
PRIMER_RIGHT_0_PENALTY=0.478496
PRIMER_LEFT_0_SEQUENCE=CTTCCCAGACATGCACACAC
PRIMER_RIGHT_0_SEQUENCE=CCCACAGCTGAATGTATCCA
PRIMER_LEFT_0=49,20
PRIMER_RIGHT_0=190,20
PRIMER_LEFT_0_TM=60.162
PRIMER_RIGHT_0_TM=59.522
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=142
PRIMER_PAIR_0_PRODUCT_TM=69.5
PRIMER_PAIR_1_PENALTY=0.640953
PRIMER_LEFT_1_PENALTY=0.162457
PRIMER_RIGHT_1_PENALTY=0.478496
PRIMER_LEFT_1_SEQUENCE=ACTTCCCAGACATGCACACA
PRIMER_RIGHT_1_SEQUENCE=CCCACAGCTGAATGTATCCA
PRIMER_LEFT_1=48,20
PRIMER_RIGHT_1=190,20
PRIMER_LEFT_1_TM=60.162
PRIMER_RIGHT_1_TM=59.522
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=143
PRIMER_PAIR_1_PRODUCT_TM=69.5
PRIMER_PAIR_2_PENALTY=1.645939
PRIMER_LEFT_2_PENALTY=1.167443
PRIMER_RIGHT_2_PENALTY=0.478496
PRIMER_LEFT_2_SEQUENCE=TACTTCCCAGACATGCACACA
PRIMER_RIGHT_2_SEQUENCE=CCCACAGCTGAATGTATCCA
PRIMER_LEFT_2=47,21
PRIMER_RIGHT_2=190,20
PRIMER_LEFT_2_TM=60.167
PRIMER_RIGHT_2_TM=59.522
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=144
PRIMER_PAIR_2_PRODUCT_TM=69.4
PRIMER_PAIR_3_PENALTY=2.061262
PRIMER_LEFT_3_PENALTY=0.162457
PRIMER_RIGHT_3_PENALTY=1.898804
PRIMER_LEFT_3_SEQUENCE=CTTCCCAGACATGCACACAC
PRIMER_RIGHT_3_SEQUENCE=CCCACAGCTGAATGTATCCAA
PRIMER_LEFT_3=49,20
PRIMER_RIGHT_3=190,21
PRIMER_LEFT_3_TM=60.162
PRIMER_RIGHT_3_TM=60.899
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=142
PRIMER_PAIR_3_PRODUCT_TM=69.5
PRIMER_PAIR_4_PENALTY=2.061262
PRIMER_LEFT_4_PENALTY=0.162457
PRIMER_RIGHT_4_PENALTY=1.898804
PRIMER_LEFT_4_SEQUENCE=ACTTCCCAGACATGCACACA
PRIMER_RIGHT_4_SEQUENCE=CCCACAGCTGAATGTATCCAA
PRIMER_LEFT_4=48,20
PRIMER_RIGHT_4=190,21
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=60.899
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=143
PRIMER_PAIR_4_PRODUCT_TM=69.5
=
SEQUENCE_ID=31.RHAP64FC4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGTTGGAGCCCAGCAAAGATGTTTTCTAGAATTCCCAACTCAGACAATCCTTGGAGGTTGTTATGTGAATGTATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACACAGGCTCATGCCTTTGTGTGGAGGTCAGAGGCATCATCTAGCTTACATTTATTAAGACAGCGTTTCACATTTTGCTTGGAATTCATTGATTCAGCTGGACTGGCTGTTTGGAGAGCCTCAGGGATCCATCCACCTGCCTCTGTCCCCTTGACCCTGGGATTACAAGCAGTCATACCCAAACTCAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAGAACTTCATCTTGTGTAGCAACTGTTTTTCTAAGCGAGATGTCT
SEQUENCE_INCLUDED_REGION=20,416
SEQUENCE_TARGET=99,48
SEQUENCE_TARGET=340,44
P3_COMMENT=CL is AGCCCAGCAAAGATGTTTTC
P3_COMMENT=CR is ATGATGCCTCTGACCTCCAC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.763816
PRIMER_LEFT_0_PENALTY=0.683672
PRIMER_RIGHT_0_PENALTY=0.080144
PRIMER_LEFT_0_SEQUENCE=AGCCCAGCAAAGATGTTTTC
PRIMER_RIGHT_0_SEQUENCE=ATGATGCCTCTGACCTCCAC
PRIMER_LEFT_0=28,20
PRIMER_RIGHT_0=189,20
PRIMER_LEFT_0_TM=59.316
PRIMER_RIGHT_0_TM=60.080
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=162
PRIMER_PAIR_0_PRODUCT_TM=68.6
PRIMER_PAIR_1_PENALTY=0.763816
PRIMER_LEFT_1_PENALTY=0.683672
PRIMER_RIGHT_1_PENALTY=0.080144
PRIMER_LEFT_1_SEQUENCE=GAGCCCAGCAAAGATGTTTT
PRIMER_RIGHT_1_SEQUENCE=ATGATGCCTCTGACCTCCAC
PRIMER_LEFT_1=27,20
PRIMER_RIGHT_1=189,20
PRIMER_LEFT_1_TM=59.316
PRIMER_RIGHT_1_TM=60.080
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=163
PRIMER_PAIR_1_PRODUCT_TM=68.8
PRIMER_PAIR_2_PENALTY=0.819572
PRIMER_LEFT_2_PENALTY=0.683672
PRIMER_RIGHT_2_PENALTY=0.135900
PRIMER_LEFT_2_SEQUENCE=AGCCCAGCAAAGATGTTTTC
PRIMER_RIGHT_2_SEQUENCE=CAAAGGCATGAGCCTGTGTA
PRIMER_LEFT_2=28,20
PRIMER_RIGHT_2=168,20
PRIMER_LEFT_2_TM=59.316
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=141
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=0.819572
PRIMER_LEFT_3_PENALTY=0.683672
PRIMER_RIGHT_3_PENALTY=0.135900
PRIMER_LEFT_3_SEQUENCE=GAGCCCAGCAAAGATGTTTT
PRIMER_RIGHT_3_SEQUENCE=CAAAGGCATGAGCCTGTGTA
PRIMER_LEFT_3=27,20
PRIMER_RIGHT_3=168,20
PRIMER_LEFT_3_TM=59.316
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=142
PRIMER_PAIR_3_PRODUCT_TM=66.9
PRIMER_PAIR_4_PENALTY=1.176036
PRIMER_LEFT_4_PENALTY=1.095892
PRIMER_RIGHT_4_PENALTY=0.080144
PRIMER_LEFT_4_SEQUENCE=TCAGACAATCCTTGGAGGTTG
PRIMER_RIGHT_4_SEQUENCE=ATGATGCCTCTGACCTCCAC
PRIMER_LEFT_4=61,21
PRIMER_RIGHT_4=189,20
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.080
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=129
PRIMER_PAIR_4_PRODUCT_TM=66.4
=
SEQUENCE_ID=33.RHAP64FC6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCCAGAACAATGTCATCTGGACAGCTTGCAAAAATGTTAGCATCTGTTCTTGGTCTACCAATGCAAGTCTATGTGGTGAAGGTGTTTGTTATTTACAGTTTAGGAAATAAGGAATAAATCTTGAGATCATCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTGGAAGTTATTGGTAAAGNGCACAGAGGCTTTCTTTTCTTTTCTTTGTTAANTTCTTTTCAAAAAGGATAAAAAAATATCTTGTCAAACTTAAAACAGCTTATGAATTCTCCCCACTTGCTTCCCAACGTACTGTTTTCTCTGTAATTATACTGAATAACAAAGATTCTATATTTGTCNCATAGTTCTNGCTACTTAC
SEQUENCE_INCLUDED_REGION=17,428
SEQUENCE_TARGET=149,40
SEQUENCE_TARGET=190,54
P3_COMMENT=CL is AAGGAATAAATCTTGAGATCATCG
P3_COMMENT=CR is AAAGAAAAGAAAGCCTCTGTGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.904598
PRIMER_LEFT_0_PENALTY=6.104219
PRIMER_RIGHT_0_PENALTY=2.800379
PRIMER_LEFT_0_SEQUENCE=AAGGAATAAATCTTGAGATCATCG
PRIMER_RIGHT_0_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
PRIMER_LEFT_0=124,24
PRIMER_RIGHT_0=287,22
PRIMER_LEFT_0_TM=57.896
PRIMER_RIGHT_0_TM=59.200
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=164
PRIMER_PAIR_0_PRODUCT_TM=62.5
PRIMER_PAIR_1_PENALTY=9.137284
PRIMER_LEFT_1_PENALTY=6.336905
PRIMER_RIGHT_1_PENALTY=2.800379
PRIMER_LEFT_1_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT
PRIMER_RIGHT_1_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
PRIMER_LEFT_1=124,25
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=58.663
PRIMER_RIGHT_1_TM=59.200
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=164
PRIMER_PAIR_1_PRODUCT_TM=62.5
PRIMER_PAIR_2_PENALTY=9.224558
PRIMER_LEFT_2_PENALTY=6.104219
PRIMER_RIGHT_2_PENALTY=3.120339
PRIMER_LEFT_2_SEQUENCE=AAGGAATAAATCTTGAGATCATCG
PRIMER_RIGHT_2_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC
PRIMER_LEFT_2=124,24
PRIMER_RIGHT_2=286,21
PRIMER_LEFT_2_TM=57.896
PRIMER_RIGHT_2_TM=57.880
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=163
PRIMER_PAIR_2_PRODUCT_TM=62.5
PRIMER_PAIR_3_PENALTY=9.382665
PRIMER_LEFT_3_PENALTY=6.582286
PRIMER_RIGHT_3_PENALTY=2.800379
PRIMER_LEFT_3_SEQUENCE=AGGAATAAATCTTGAGATCATCGT
PRIMER_RIGHT_3_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
PRIMER_LEFT_3=125,24
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=57.418
PRIMER_RIGHT_3_TM=59.200
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=163
PRIMER_PAIR_3_PRODUCT_TM=62.5
PRIMER_PAIR_4_PENALTY=9.457244
PRIMER_LEFT_4_PENALTY=6.336905
PRIMER_RIGHT_4_PENALTY=3.120339
PRIMER_LEFT_4_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT
PRIMER_RIGHT_4_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC
PRIMER_LEFT_4=124,25
PRIMER_RIGHT_4=286,21
PRIMER_LEFT_4_TM=58.663
PRIMER_RIGHT_4_TM=57.880
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=163
PRIMER_PAIR_4_PRODUCT_TM=62.5
=
SEQUENCE_ID=34.RHAP64FD10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCACTAGGGAGCCTTTATCAAATAAGTAGCTTGACCAAATGCCTTTTCAGTACNNNNNNNNNNNNNNNGCCTTCTCTTTTCTGTATCGGACTATACTTCCCTGAGAAATTCAGAAGAAATTGAAAGGTGAACTACTTCACCATCTCCCCTCTGCGTGCATCCATCTGTTACAAAGGATATCGTTTGACATATATAACCATGTTAACCTTCACGATTTTAAGGAAGTCCTTGGTGGAGGACCACAAAGAATAGATTGACCTCAAAAGGAGGTCTATTGGTCTGAAAAGTTGGCACCCCCAAGTCCTGTGGCTGGGTACCCAAGGATACAAGAAAAATCACCTCGAAGGGTCTGAAAATCAGTTTTGTAGTTAGGAGGAAGAGCNTGGTAAACTGCTCTTCAAAACTACCTGATAGGGGAANATGAGTCCATTGTCTGGTTTGTTT
SEQUENCE_INCLUDED_REGION=24,443
SEQUENCE_TARGET=76,15
P3_COMMENT=CL is GCTTGACCAAATGCCTTTTC
P3_COMMENT=CR is GTAACAGATGGATGCACGCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.006754
PRIMER_LEFT_0_PENALTY=0.309288
PRIMER_RIGHT_0_PENALTY=0.697466
PRIMER_LEFT_0_SEQUENCE=GCTTGACCAAATGCCTTTTC
PRIMER_RIGHT_0_SEQUENCE=GTAACAGATGGATGCACGCA
PRIMER_LEFT_0=51,20
PRIMER_RIGHT_0=193,20
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=60.697
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=143
PRIMER_PAIR_0_PRODUCT_TM=71.8
PRIMER_PAIR_1_PENALTY=1.006754
PRIMER_LEFT_1_PENALTY=0.309288
PRIMER_RIGHT_1_PENALTY=0.697466
PRIMER_LEFT_1_SEQUENCE=GCTTGACCAAATGCCTTTTC
PRIMER_RIGHT_1_SEQUENCE=TGTAACAGATGGATGCACGC
PRIMER_LEFT_1=51,20
PRIMER_RIGHT_1=194,20
PRIMER_LEFT_1_TM=59.691
PRIMER_RIGHT_1_TM=60.697
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=144
PRIMER_PAIR_1_PRODUCT_TM=71.7
PRIMER_PAIR_2_PENALTY=1.142819
PRIMER_LEFT_2_PENALTY=0.309288
PRIMER_RIGHT_2_PENALTY=0.833530
PRIMER_LEFT_2_SEQUENCE=GCTTGACCAAATGCCTTTTC
PRIMER_RIGHT_2_SEQUENCE=TAACAGATGGATGCACGCAG
PRIMER_LEFT_2=51,20
PRIMER_RIGHT_2=192,20
PRIMER_LEFT_2_TM=59.691
PRIMER_RIGHT_2_TM=60.834
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=142
PRIMER_PAIR_2_PRODUCT_TM=71.6
PRIMER_PAIR_3_PENALTY=1.208202
PRIMER_LEFT_3_PENALTY=0.309288
PRIMER_RIGHT_3_PENALTY=0.898913
PRIMER_LEFT_3_SEQUENCE=GCTTGACCAAATGCCTTTTC
PRIMER_RIGHT_3_SEQUENCE=CAGAGGGGAGATGGTGAAGT
PRIMER_LEFT_3=51,20
PRIMER_RIGHT_3=175,20
PRIMER_LEFT_3_TM=59.691
PRIMER_RIGHT_3_TM=59.101
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=125
PRIMER_PAIR_3_PRODUCT_TM=70.5
PRIMER_PAIR_4_PENALTY=1.510507
PRIMER_LEFT_4_PENALTY=0.309288
PRIMER_RIGHT_4_PENALTY=1.201218
PRIMER_LEFT_4_SEQUENCE=GCTTGACCAAATGCCTTTTC
PRIMER_RIGHT_4_SEQUENCE=TAACAGATGGATGCACGCA
PRIMER_LEFT_4=51,20
PRIMER_RIGHT_4=192,19
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=59.799
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.368
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=142
PRIMER_PAIR_4_PRODUCT_TM=71.6
=
SEQUENCE_ID=35.RHAP64FD11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTCCTGAGAAATGCAGGTCAGGGTACCTTGGCAGGGAATCCCAAGGATCAGTACATGGCAGCTGAAAGTTGTCTTGGATGCAAGACTGGGAGTGAGGCTCAAGTCAGCCGAGCCTCAGCTCTGGATTCCTGAAACAGAATACCAGAATATGTTAGAGAGCGAGGAGACTAAACTAAACATCTGGGAGTCCTCAGCAGATTGTACAGATCCAGGGCTGAGGGNTCATAAGTATGTTGGTCTTTGTGGGGGTGGGAAAGGAACACTTATCTAGATCCCCACCTCCTTACTATGTGCTACTACGGGTGGNGGCAATGCCTTAAGGACCAAGGTTTTTTGTTTTGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGTGTGTGNGTTGCATNNAATGCANGGNGCAGGTGGTANTAATTGNNGGGGGNTAANNCANTGTTCTTCNTCTACCNNNTTCC
SEQUENCE_INCLUDED_REGION=23,460
SEQUENCE_TARGET=369,28
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=36.RHAP64FD6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCACATTTTTCTTTANGNNNNNNNANTGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCGNNAAGGCACAANTAACCAAGGANGGAGGTCAGACAACAAGTTTATGGCGTCAATTCNCTCTCACCTTTACATGGATNGCCNGNNNTACACTGCAAACATCCGGCACTGNACCGTTCTGCCAGTCCANGGTCTCCCNANNTTTGTCCCCCTCNCTGTCTCNCNGTCTCTGCCCTTGNTAGTCTGNCGNNNCTCCGATANCCTCNNNACCCCNGGNNTTCTGCTCTCNGTGACTTCCCCCTCNCCCTGNCTCCCNCNGTCNGNTCCGTCTNGTCCNTGCNNTTNCCTAGTTTCNCNCCNNTNNGTNTCCCNGGNNTGGCGNGGTCNGNNNGTGCCNCCNNNTATNGTGA
SEQUENCE_INCLUDED_REGION=27,434
SEQUENCE_TARGET=57,46
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=37.RHAP64FE4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCAGCCTTTGATACAGGAACAGGAGGACCCAGTCCGTAAAACCAAAGGTGAGGGAGAAACTAAGACATTCTCAGATAAAGAAACTAAGGAAATGTCTTGATAATGTAACAAGCCTTGGAAACAGCTTTGGCTACAAAGCACCGCTTCCTCTCATGGAACCCACAGCAATCGTTGGCAAGGGCAGTGAGACAGACGGCTCAGCAGTTCTAAGTACTCGGTGACCTGAATTTAACCCACATGGAAGACGGCAACAATCAACCCTTGAAAGTCTTCTGAGTGTGTACCCTGGCACCAAAGGACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGGTTTTTCTTTTTTCTTTTTCTTTTTTTAAAGTAGAAGCAGGCAGCCGGAAGTGGTANCTCACAATCTTTATCTTTT
SEQUENCE_INCLUDED_REGION=20,428
SEQUENCE_TARGET=322,44
P3_COMMENT=CL is TCTGAGTGTGTACCCTGGCA
P3_COMMENT=CR is CGGCTGCCTGCTTCTACTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.992996
PRIMER_LEFT_0_PENALTY=0.309402
PRIMER_RIGHT_0_PENALTY=1.683594
PRIMER_LEFT_0_SEQUENCE=TCTGAGTGTGTACCCTGGCA
PRIMER_RIGHT_0_SEQUENCE=CGGCTGCCTGCTTCTACTT
PRIMER_LEFT_0=291,20
PRIMER_RIGHT_0=418,19
PRIMER_LEFT_0_TM=60.309
PRIMER_RIGHT_0_TM=60.684
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=128
PRIMER_PAIR_0_PRODUCT_TM=65.5
PRIMER_PAIR_1_PENALTY=2.067393
PRIMER_LEFT_1_PENALTY=0.383798
PRIMER_RIGHT_1_PENALTY=1.683594
PRIMER_LEFT_1_SEQUENCE=ACATGGAAGACGGCAACAAT
PRIMER_RIGHT_1_SEQUENCE=CGGCTGCCTGCTTCTACTT
PRIMER_LEFT_1=255,20
PRIMER_RIGHT_1=418,19
PRIMER_LEFT_1_TM=60.384
PRIMER_RIGHT_1_TM=60.684
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=164
PRIMER_PAIR_1_PRODUCT_TM=68.2
PRIMER_PAIR_2_PENALTY=2.187940
PRIMER_LEFT_2_PENALTY=0.504346
PRIMER_RIGHT_2_PENALTY=1.683594
PRIMER_LEFT_2_SEQUENCE=GAAGACGGCAACAATCAACC
PRIMER_RIGHT_2_SEQUENCE=CGGCTGCCTGCTTCTACTT
PRIMER_LEFT_2=260,20
PRIMER_RIGHT_2=418,19
PRIMER_LEFT_2_TM=60.504
PRIMER_RIGHT_2_TM=60.684
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=159
PRIMER_PAIR_2_PRODUCT_TM=68.0
PRIMER_PAIR_3_PENALTY=2.187940
PRIMER_LEFT_3_PENALTY=0.504346
PRIMER_RIGHT_3_PENALTY=1.683594
PRIMER_LEFT_3_SEQUENCE=GGAAGACGGCAACAATCAAC
PRIMER_RIGHT_3_SEQUENCE=CGGCTGCCTGCTTCTACTT
PRIMER_LEFT_3=259,20
PRIMER_RIGHT_3=418,19
PRIMER_LEFT_3_TM=60.504
PRIMER_RIGHT_3_TM=60.684
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=160
PRIMER_PAIR_3_PRODUCT_TM=68.2
PRIMER_PAIR_4_PENALTY=2.238002
PRIMER_LEFT_4_PENALTY=0.309402
PRIMER_RIGHT_4_PENALTY=1.928601
PRIMER_LEFT_4_SEQUENCE=TCTGAGTGTGTACCCTGGCA
PRIMER_RIGHT_4_SEQUENCE=CGGCTGCCTGCTTCTACTTT
PRIMER_LEFT_4=291,20
PRIMER_RIGHT_4=418,20
PRIMER_LEFT_4_TM=60.309
PRIMER_RIGHT_4_TM=61.929
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=128
PRIMER_PAIR_4_PRODUCT_TM=65.5
=
SEQUENCE_ID=38.RHAP64FE5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCATGTAGAATGTGTTTGTTTGTTTTTGTAAACTTAACTTAATCAAGGATATTCCTCACAGGCATACTCAGAAGATAATCTACAAAATCCCTCACATGATGTAGACANGTGGTCTACATCAGTAGGTCTCCACACTCTAGTGGCTGTTTTCTCCAGGTAGTGCTTAACTCTGGCTTTTAGCTGTTTCTAAATATTTCCTGGGAAAATTAAGGTTAACGAGAAACATATTGACCTTCGNTGTTCTTCCTGTGTGGATAATCTGACTTTGGACAGACCTAGGTTGTGAGGAATGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTGCGCGTGCCAGAGGTCGACCACAGCACAGGTGTTCTTTCTCAGAAGCAATCTACCNTATTTTATTTTATNC
SEQUENCE_INCLUDED_REGION=17,424
SEQUENCE_TARGET=310,56
P3_COMMENT=CL is CCTAGGTTGTGAGGAATGCC
P3_COMMENT=CR is TGCTTCTGAGAAAGAACACCTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.793510
PRIMER_LEFT_0_PENALTY=0.449829
PRIMER_RIGHT_0_PENALTY=2.343680
PRIMER_LEFT_0_SEQUENCE=CCTAGGTTGTGAGGAATGCC
PRIMER_RIGHT_0_SEQUENCE=TGCTTCTGAGAAAGAACACCTG
PRIMER_LEFT_0=290,20
PRIMER_RIGHT_0=417,22
PRIMER_LEFT_0_TM=59.550
PRIMER_RIGHT_0_TM=59.656
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=128
PRIMER_PAIR_0_PRODUCT_TM=68.7
PRIMER_PAIR_1_PENALTY=2.929880
PRIMER_LEFT_1_PENALTY=0.449829
PRIMER_RIGHT_1_PENALTY=2.480050
PRIMER_LEFT_1_SEQUENCE=CCTAGGTTGTGAGGAATGCC
PRIMER_RIGHT_1_SEQUENCE=TTCTGAGAAAGAACACCTGTGC
PRIMER_LEFT_1=290,20
PRIMER_RIGHT_1=414,22
PRIMER_LEFT_1_TM=59.550
PRIMER_RIGHT_1_TM=59.520
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_1_PRODUCT_TM=68.2
PRIMER_PAIR_2_PENALTY=3.566148
PRIMER_LEFT_2_PENALTY=2.343332
PRIMER_RIGHT_2_PENALTY=1.222816
PRIMER_LEFT_2_SEQUENCE=GGACAGACCTAGGTTGTGAGG
PRIMER_RIGHT_2_SEQUENCE=AAAGAACACCTGTGCTGTGG
PRIMER_LEFT_2=283,21
PRIMER_RIGHT_2=407,20
PRIMER_LEFT_2_TM=58.657
PRIMER_RIGHT_2_TM=58.777
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=125
PRIMER_PAIR_2_PRODUCT_TM=68.6
PRIMER_PAIR_3_PENALTY=3.579314
PRIMER_LEFT_3_PENALTY=2.366796
PRIMER_RIGHT_3_PENALTY=1.212518
PRIMER_LEFT_3_SEQUENCE=TTTGGACAGACCTAGGTTGTGA
PRIMER_RIGHT_3_SEQUENCE=AAGAACACCTGTGCTGTGGTC
PRIMER_LEFT_3=280,22
PRIMER_RIGHT_3=406,21
PRIMER_LEFT_3_TM=59.633
PRIMER_RIGHT_3_TM=60.213
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=68.4
PRIMER_PAIR_4_PENALTY=3.589613
PRIMER_LEFT_4_PENALTY=2.366796
PRIMER_RIGHT_4_PENALTY=1.222816
PRIMER_LEFT_4_SEQUENCE=TTTGGACAGACCTAGGTTGTGA
PRIMER_RIGHT_4_SEQUENCE=AAAGAACACCTGTGCTGTGG
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=407,20
PRIMER_LEFT_4_TM=59.633
PRIMER_RIGHT_4_TM=58.777
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=10.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=128
PRIMER_PAIR_4_PRODUCT_TM=68.3
=
SEQUENCE_ID=39.RHAP64FE12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNACCCCCCANAGCGTCCGTCTCNGNNNNNNNNNNNNNNNNNNNNNNNATGNANNNNNNNNNNNNNNNNNNTANGCCNGNNNNNNNNNNNNNNNNNNNNNNNATATGTCNGTGTGCCNCNNNNNNNNNNNNNNNNNNTATATCNGTGTATATGTGNGTGTACCCNNATGTGTGNGTACNCCTGTCNGTGNATGTGTGTATGNCTCNGCACCNCNCNNNCNCNCTNNATGNCNNNATANNTANCTCNANGTGNNCGCCCCNATGNACATNNCNGTGCCNACANNTCNGNATNCGNCCCNCNATCTCNANCNANGTCNGNNNNNANGNNNGCATATCNNNGTANNCNNANNTCNNTCNATACCNATCTANNNGNNNNNANCNANNNNNCNNNNNATANCCNCNCNCNGTCNCCNATNTANACANNNANNNNTNNGT
SEQUENCE_INCLUDED_REGION=23,432
SEQUENCE_TARGET=47,22
SEQUENCE_TARGET=74,18
SEQUENCE_TARGET=101,22
SEQUENCE_TARGET=140,18
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=39.RHAP64FE6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCCTTTACTTGAATGAAAAGTATTGTTTCTGTCTGTCTCTGTCTCTTTGTCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGNAGGGNGCGTTACCCACCATAGATTATCCCCGACAATTTCAGTCTTCTCTGAGAATATCATAGTANAGACCCACCAAAATTGTCTCATTTNCAACACTGATCTTGCAAAGAAAATTAACATAAATCCGNNAAACTAATACTGAATANTTATAATATATCCATATCATGTCAACTATTTACATTCATAGAGTCCTCATCTAGAAAAACAATANTCTTTGTTTNTACAGTGGTTTGTACATGGC
SEQUENCE_INCLUDED_REGION=16,425
SEQUENCE_TARGET=70,36
SEQUENCE_TARGET=107,54
SEQUENCE_TARGET=162,32
P3_COMMENT=CL is TTCTGTCTGTCTCTGTCTCTTTGTC
P3_COMMENT=CR is TCGGGGATAATCTATGGTGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.162646
PRIMER_LEFT_0_PENALTY=5.761718
PRIMER_RIGHT_0_PENALTY=0.400928
PRIMER_LEFT_0_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
PRIMER_RIGHT_0_SEQUENCE=TCGGGGATAATCTATGGTGG
PRIMER_LEFT_0=41,25
PRIMER_RIGHT_0=231,20
PRIMER_LEFT_0_TM=59.238
PRIMER_RIGHT_0_TM=59.599
PRIMER_LEFT_0_GC_PERCENT=44.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=1.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=191
PRIMER_PAIR_0_PRODUCT_TM=64.1
PRIMER_PAIR_1_PENALTY=6.235412
PRIMER_LEFT_1_PENALTY=5.761718
PRIMER_RIGHT_1_PENALTY=0.473695
PRIMER_LEFT_1_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
PRIMER_RIGHT_1_SEQUENCE=AAGACTGAAATTGTCGGGGA
PRIMER_LEFT_1=41,25
PRIMER_RIGHT_1=244,20
PRIMER_LEFT_1_TM=59.238
PRIMER_RIGHT_1_TM=59.526
PRIMER_LEFT_1_GC_PERCENT=44.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=204
PRIMER_PAIR_1_PRODUCT_TM=64.6
PRIMER_PAIR_2_PENALTY=6.235412
PRIMER_LEFT_2_PENALTY=5.761718
PRIMER_RIGHT_2_PENALTY=0.473695
PRIMER_LEFT_2_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
PRIMER_RIGHT_2_SEQUENCE=GAAGACTGAAATTGTCGGGG
PRIMER_LEFT_2=41,25
PRIMER_RIGHT_2=245,20
PRIMER_LEFT_2_TM=59.238
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=44.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=205
PRIMER_PAIR_2_PRODUCT_TM=64.8
PRIMER_PAIR_3_PENALTY=6.473621
PRIMER_LEFT_3_PENALTY=6.072692
PRIMER_RIGHT_3_PENALTY=0.400928
PRIMER_LEFT_3_SEQUENCE=TCTGTCTCTGTCTCTTTGTCTTTG
PRIMER_RIGHT_3_SEQUENCE=TCGGGGATAATCTATGGTGG
PRIMER_LEFT_3=46,24
PRIMER_RIGHT_3=231,20
PRIMER_LEFT_3_TM=57.927
PRIMER_RIGHT_3_TM=59.599
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=186
PRIMER_PAIR_3_PRODUCT_TM=63.7
PRIMER_PAIR_4_PENALTY=6.473621
PRIMER_LEFT_4_PENALTY=6.072692
PRIMER_RIGHT_4_PENALTY=0.400928
PRIMER_LEFT_4_SEQUENCE=TTTCTGTCTGTCTCTGTCTCTTTG
PRIMER_RIGHT_4_SEQUENCE=TCGGGGATAATCTATGGTGG
PRIMER_LEFT_4=40,24
PRIMER_RIGHT_4=231,20
PRIMER_LEFT_4_TM=57.927
PRIMER_RIGHT_4_TM=59.599
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=192
PRIMER_PAIR_4_PRODUCT_TM=64.0
=
SEQUENCE_ID=41.RHAP64FF11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCATGATATGGGCGTGGTTTGTGTGCTTGCTAAGTCAGTGAAGCACTCTCTTGAAGGAAGGAGGACCTGAATACAATCCCCCAGAATCTATGATGAGTTGGAAGCAGAACTGTCCATAGTTTTGCAAGACTGTATTTCTTGTGCTGGTATGATGGAGTCACATGATCCATTCAATCATTTGCCAGCCAGACTAACCTCACTAGTTAAGTTTAAGGCTGATAAGGGACCAAATATTAAAAAGAAAGTTGATTTCCAGGAGGAACATCTGAGGTTGTCTTTTAGCCAGTACACATTTGTTTGCAAATATGCACACATACTAGAGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGAATGGGGGGAGTTCAGAAAATCTCCAGGGATCNGTTNTNA
SEQUENCE_INCLUDED_REGION=23,446
SEQUENCE_TARGET=348,74
P3_COMMENT=CL is TCCAGGAGGAACATCTGAGG
P3_COMMENT=CR is TTCTGAACTCCCCCCATTC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.343959
PRIMER_LEFT_0_PENALTY=0.190960
PRIMER_RIGHT_0_PENALTY=1.152999
PRIMER_LEFT_0_SEQUENCE=TCCAGGAGGAACATCTGAGG
PRIMER_RIGHT_0_SEQUENCE=TTCTGAACTCCCCCCATTC
PRIMER_LEFT_0=273,20
PRIMER_RIGHT_0=446,19
PRIMER_LEFT_0_TM=60.191
PRIMER_RIGHT_0_TM=59.847
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=52.632
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=67.3
PRIMER_PAIR_1_PENALTY=1.382688
PRIMER_LEFT_1_PENALTY=0.190960
PRIMER_RIGHT_1_PENALTY=1.191728
PRIMER_LEFT_1_SEQUENCE=TCCAGGAGGAACATCTGAGG
PRIMER_RIGHT_1_SEQUENCE=TTTCTGAACTCCCCCCATTC
PRIMER_LEFT_1=273,20
PRIMER_RIGHT_1=447,20
PRIMER_LEFT_1_TM=60.191
PRIMER_RIGHT_1_TM=61.192
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=67.3
PRIMER_PAIR_2_PENALTY=1.562074
PRIMER_LEFT_2_PENALTY=0.898913
PRIMER_RIGHT_2_PENALTY=0.663161
PRIMER_LEFT_2_SEQUENCE=CCAGGAGGAACATCTGAGGT
PRIMER_RIGHT_2_SEQUENCE=TTTTCTGAACTCCCCCCATT
PRIMER_LEFT_2=274,20
PRIMER_RIGHT_2=448,20
PRIMER_LEFT_2_TM=59.101
PRIMER_RIGHT_2_TM=60.663
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=175
PRIMER_PAIR_2_PRODUCT_TM=67.3
PRIMER_PAIR_3_PENALTY=1.665234
PRIMER_LEFT_3_PENALTY=1.293870
PRIMER_RIGHT_3_PENALTY=0.371364
PRIMER_LEFT_3_SEQUENCE=CAGGAGGAACATCTGAGGTTG
PRIMER_RIGHT_3_SEQUENCE=ATTTTCTGAACTCCCCCCAT
PRIMER_LEFT_3=275,21
PRIMER_RIGHT_3=449,20
PRIMER_LEFT_3_TM=59.706
PRIMER_RIGHT_3_TM=59.629
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=175
PRIMER_PAIR_3_PRODUCT_TM=67.0
PRIMER_PAIR_4_PENALTY=1.724434
PRIMER_LEFT_4_PENALTY=1.353069
PRIMER_RIGHT_4_PENALTY=0.371364
PRIMER_LEFT_4_SEQUENCE=GCCAGTACACATTTGTTTGCA
PRIMER_RIGHT_4_SEQUENCE=ATTTTCTGAACTCCCCCCAT
PRIMER_LEFT_4=303,21
PRIMER_RIGHT_4=449,20
PRIMER_LEFT_4_TM=59.647
PRIMER_RIGHT_4_TM=59.629
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=147
PRIMER_PAIR_4_PRODUCT_TM=65.0
=
SEQUENCE_ID=44.RHAP64FG5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCCTCTTTCCTCTGTAGAAAATGCACTGCTAATAACTTTGCTCAAATATATCTCACAACATTTCTTAATATGGCTCCAGGTAGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNACAGACAGACAGACAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAAGAGTGAAACCTAGGTACCCTGGTATTATAAAGAACACATTTTCAGCTCAAAAGCGAGCAGGGNGTCGCTCCACTCTCCATGGGTCTGGGTGTGGCTCCNTCNCCACACTGAAGGGTCTGNTGAGCTCCTGNAGGGGTAACAGGGGCTGCNTCCTCANATTANGGGTTCAGAGGNTGGNGGGTGNGCCACATTTTTTCAAANACA
SEQUENCE_INCLUDED_REGION=17,435
SEQUENCE_TARGET=103,40
SEQUENCE_TARGET=144,22
SEQUENCE_TARGET=186,54
P3_COMMENT=CL is TCCTCTGTAGAAAATGCACTGC
P3_COMMENT=CR is CAGACCCATGGAGAGTGGAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.146047
PRIMER_LEFT_0_PENALTY=2.478252
PRIMER_RIGHT_0_PENALTY=0.667795
PRIMER_LEFT_0_SEQUENCE=TCCTCTGTAGAAAATGCACTGC
PRIMER_RIGHT_0_SEQUENCE=CAGACCCATGGAGAGTGGAG
PRIMER_LEFT_0=23,22
PRIMER_RIGHT_0=334,20
PRIMER_LEFT_0_TM=59.522
PRIMER_RIGHT_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=312
PRIMER_PAIR_0_PRODUCT_TM=69.1
PRIMER_PAIR_1_PENALTY=3.488781
PRIMER_LEFT_1_PENALTY=2.478252
PRIMER_RIGHT_1_PENALTY=1.010529
PRIMER_LEFT_1_SEQUENCE=TCCTCTGTAGAAAATGCACTGC
PRIMER_RIGHT_1_SEQUENCE=TCGCTTTTGAGCTGAAAATGT
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=303,21
PRIMER_LEFT_1_TM=59.522
PRIMER_RIGHT_1_TM=60.011
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=281
PRIMER_PAIR_1_PRODUCT_TM=67.3
PRIMER_PAIR_2_PENALTY=3.756457
PRIMER_LEFT_2_PENALTY=3.088662
PRIMER_RIGHT_2_PENALTY=0.667795
PRIMER_LEFT_2_SEQUENCE=TGCACTGCTAATAACTTTGCTCA
PRIMER_RIGHT_2_SEQUENCE=CAGACCCATGGAGAGTGGAG
PRIMER_LEFT_2=37,23
PRIMER_RIGHT_2=334,20
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=60.668
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=298
PRIMER_PAIR_2_PRODUCT_TM=68.9
PRIMER_PAIR_3_PENALTY=3.789280
PRIMER_LEFT_3_PENALTY=3.121486
PRIMER_RIGHT_3_PENALTY=0.667795
PRIMER_LEFT_3_SEQUENCE=TCTTAATATGGCTCCAGGTAGCA
PRIMER_RIGHT_3_SEQUENCE=CAGACCCATGGAGAGTGGAG
PRIMER_LEFT_3=78,23
PRIMER_RIGHT_3=334,20
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=60.668
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=257
PRIMER_PAIR_3_PRODUCT_TM=68.3
PRIMER_PAIR_4_PENALTY=3.815878
PRIMER_LEFT_4_PENALTY=3.148084
PRIMER_RIGHT_4_PENALTY=0.667795
PRIMER_LEFT_4_SEQUENCE=AATATGGCTCCAGGTAGCAGT
PRIMER_RIGHT_4_SEQUENCE=CAGACCCATGGAGAGTGGAG
PRIMER_LEFT_4=82,21
PRIMER_RIGHT_4=334,20
PRIMER_LEFT_4_TM=57.852
PRIMER_RIGHT_4_TM=60.668
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=253
PRIMER_PAIR_4_PRODUCT_TM=68.2
=
SEQUENCE_ID=45.RHAP64FG12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTGTCAATATTCCAGAACAGCGCATGCCACCCACACAACGTGCTTCAGCCAGCCCTGCAGTGCCCTTCCTGGACAGGATTTCTTCTTCTTTGAAAGAAAAACAACAACAAAGCTTTTATTATANNTATCNAATTNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTATTTGGACATAAAGATGAGACTGGTAGGAAGCTTTGTGTTGGAGACTGTCCTTAAATTCCCTCCATCTTTCCTTCAGGGGAATAGCCCATGCCTGTGGAACCCATCGCTCCTCACTGGGTAGCTGTCAGAAGTGTGAGGATGCTAACAGTCTGTAAACGGTGCTGTCCCTGCAGCGATCCCCAGCAGAGTCTGAGAAACTCCCAATCCACCAGCAAGATGACACAATTGTTTTGCTG
SEQUENCE_INCLUDED_REGION=22,442
SEQUENCE_TARGET=156,14
SEQUENCE_TARGET=172,50
P3_COMMENT=CL is TTGAAAGAAAAACAACAACAAAGC
P3_COMMENT=CR is CCAACACAAAGCTTCCTACCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.419108
PRIMER_LEFT_0_PENALTY=4.265519
PRIMER_RIGHT_0_PENALTY=1.153589
PRIMER_LEFT_0_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC
PRIMER_RIGHT_0_SEQUENCE=CCAACACAAAGCTTCCTACCA
PRIMER_LEFT_0=111,24
PRIMER_RIGHT_0=268,21
PRIMER_LEFT_0_TM=59.734
PRIMER_RIGHT_0_TM=60.154
PRIMER_LEFT_0_GC_PERCENT=29.167
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=158
PRIMER_PAIR_0_PRODUCT_TM=62.9
PRIMER_PAIR_1_PENALTY=5.505495
PRIMER_LEFT_1_PENALTY=4.265519
PRIMER_RIGHT_1_PENALTY=1.239976
PRIMER_LEFT_1_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC
PRIMER_RIGHT_1_SEQUENCE=GGACAGTCTCCAACACAAAGC
PRIMER_LEFT_1=111,24
PRIMER_RIGHT_1=277,21
PRIMER_LEFT_1_TM=59.734
PRIMER_RIGHT_1_TM=59.760
PRIMER_LEFT_1_GC_PERCENT=29.167
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=167
PRIMER_PAIR_1_PRODUCT_TM=63.9
PRIMER_PAIR_2_PENALTY=5.570866
PRIMER_LEFT_2_PENALTY=0.821928
PRIMER_RIGHT_2_PENALTY=4.748938
PRIMER_LEFT_2_SEQUENCE=CCCTTCCTGGACAGGATTTC
PRIMER_RIGHT_2_SEQUENCE=CCAGTCTCATCTTTATGTCCAAA
PRIMER_LEFT_2=84,20
PRIMER_RIGHT_2=250,23
PRIMER_LEFT_2_TM=60.822
PRIMER_RIGHT_2_TM=58.251
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=167
PRIMER_PAIR_2_PRODUCT_TM=63.9
PRIMER_PAIR_3_PENALTY=5.638731
PRIMER_LEFT_3_PENALTY=4.485142
PRIMER_RIGHT_3_PENALTY=1.153589
PRIMER_LEFT_3_SEQUENCE=TGAAAGAAAAACAACAACAAAGC
PRIMER_RIGHT_3_SEQUENCE=CCAACACAAAGCTTCCTACCA
PRIMER_LEFT_3=112,23
PRIMER_RIGHT_3=268,21
PRIMER_LEFT_3_TM=58.515
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=30.435
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=62.9
PRIMER_PAIR_4_PENALTY=5.725118
PRIMER_LEFT_4_PENALTY=4.485142
PRIMER_RIGHT_4_PENALTY=1.239976
PRIMER_LEFT_4_SEQUENCE=TGAAAGAAAAACAACAACAAAGC
PRIMER_RIGHT_4_SEQUENCE=GGACAGTCTCCAACACAAAGC
PRIMER_LEFT_4=112,23
PRIMER_RIGHT_4=277,21
PRIMER_LEFT_4_TM=58.515
PRIMER_RIGHT_4_TM=59.760
PRIMER_LEFT_4_GC_PERCENT=30.435
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=166
PRIMER_PAIR_4_PRODUCT_TM=63.9
=
SEQUENCE_ID=46.RHAP64FH10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCCAGCCCTGCTAACCACCTAAAGGCACCAGCAGGAGCTNCCCTCAAAAGATCTCACGTTTCCTCTGACAGCACCTTAAAGGAGCACCANTAATGAAGGCTGCTCTGTTGNGTGGGTAGGATGCCAAGGCAGAGGCGGCTTTCTACAGAGCTCCTTCCTCGCCGCAGTAACTCAAGTAGACACCTCAGTCGTTCTCCTGCAGTGTTCTGTGTTCATCACAAGGGGGCAGCCTTGACCCACTTCCCATGGTTGCCAATTCTCCAACCCATCCCCCTCCCCTCATCTAAGAAGACCTTGAACCTGGAGGAGAATATAAATAAACCTGAAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNATGNGTNTGNNTGGTTAGNGTAAANATGGGGTATGNNNNNNTGGNGNGTTAGANNNANGGTGAGNNTATNTGNATGT
SEQUENCE_INCLUDED_REGION=12,456
SEQUENCE_TARGET=343,26
SEQUENCE_TARGET=371,20
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=46.RHAP64FH4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCAGCTAGCCTGGCACACACAACAAAACAAGAACCCTTGCTTGAATAAAATGGAAGGTGAGGTCTGATACCCAAAATTAACCTTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGNTATTTTTATAANATAGAGAAAAGNAATTTCATTAAAAGGAANTGAAAGAAGTNTAAATATAATTATANATTTTAGATTAAAANTTTGTAATNAAAGCACACGGTATGTGTTTATTTAAGTGAGAGTACACGTGGAGTTAAAATGTGGATATANATAAAAATNAGNGAGNTTTTAAAAATNANTTTCTTAGNTTGNTTTAGGGATATNTNTNNACCACAAACANATACANAAAACCCAAAANAAANNNNNNNNNNNNNNNNNAGNA
SEQUENCE_INCLUDED_REGION=19,433
SEQUENCE_TARGET=107,18
SEQUENCE_TARGET=126,22
SEQUENCE_TARGET=151,32
SEQUENCE_TARGET=433,15
P3_COMMENT=CL is CCTGGCACACACAACAAAAC
P3_COMMENT=CR is TCCACATTTTAACTCCACGTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.508517
PRIMER_LEFT_0_PENALTY=0.048588
PRIMER_RIGHT_0_PENALTY=2.459929
PRIMER_LEFT_0_SEQUENCE=CCTGGCACACACAACAAAAC
PRIMER_RIGHT_0_SEQUENCE=TCCACATTTTAACTCCACGTG
PRIMER_LEFT_0=27,20
PRIMER_RIGHT_0=334,21
PRIMER_LEFT_0_TM=60.049
PRIMER_RIGHT_0_TM=58.540
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=308
PRIMER_PAIR_0_PRODUCT_TM=66.7
PRIMER_PAIR_1_PENALTY=2.647262
PRIMER_LEFT_1_PENALTY=0.048588
PRIMER_RIGHT_1_PENALTY=2.598673
PRIMER_LEFT_1_SEQUENCE=CCTGGCACACACAACAAAAC
PRIMER_RIGHT_1_SEQUENCE=TCCACATTTTAACTCCACGTGT
PRIMER_LEFT_1=27,20
PRIMER_RIGHT_1=334,22
PRIMER_LEFT_1_TM=60.049
PRIMER_RIGHT_1_TM=59.401
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=308
PRIMER_PAIR_1_PRODUCT_TM=66.7
PRIMER_PAIR_2_PENALTY=2.851188
PRIMER_LEFT_2_PENALTY=0.391259
PRIMER_RIGHT_2_PENALTY=2.459929
PRIMER_LEFT_2_SEQUENCE=TGGCACACACAACAAAACAA
PRIMER_RIGHT_2_SEQUENCE=TCCACATTTTAACTCCACGTG
PRIMER_LEFT_2=29,20
PRIMER_RIGHT_2=334,21
PRIMER_LEFT_2_TM=59.609
PRIMER_RIGHT_2_TM=58.540
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=306
PRIMER_PAIR_2_PRODUCT_TM=66.5
PRIMER_PAIR_3_PENALTY=2.989933
PRIMER_LEFT_3_PENALTY=0.391259
PRIMER_RIGHT_3_PENALTY=2.598673
PRIMER_LEFT_3_SEQUENCE=TGGCACACACAACAAAACAA
PRIMER_RIGHT_3_SEQUENCE=TCCACATTTTAACTCCACGTGT
PRIMER_LEFT_3=29,20
PRIMER_RIGHT_3=334,22
PRIMER_LEFT_3_TM=59.609
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=306
PRIMER_PAIR_3_PRODUCT_TM=66.5
PRIMER_PAIR_4_PENALTY=3.166398
PRIMER_LEFT_4_PENALTY=0.048588
PRIMER_RIGHT_4_PENALTY=3.117810
PRIMER_LEFT_4_SEQUENCE=CCTGGCACACACAACAAAAC
PRIMER_RIGHT_4_SEQUENCE=ATCCACATTTTAACTCCACGTG
PRIMER_LEFT_4=27,20
PRIMER_RIGHT_4=335,22
PRIMER_LEFT_4_TM=60.049
PRIMER_RIGHT_4_TM=58.882
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=309
PRIMER_PAIR_4_PRODUCT_TM=66.7
=
SEQUENCE_ID=06.RHAP68FB3.seq
SEQUENCE_TEMPLATE=NNNNNNNNACACTTGCTTTTGGTAGGTAAAACGCATGGAATTTGATTCCAAACATGACACAAAAGTTCACCTTATTTCCAACTCATTTCATGCACTTGTGTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAACCTTTGGGTTTAGCTACATTTAGTTGGTTCTGTTCCTTGCAACTGAAACATTGAGATTTTTCCCACAAGGAAAGAAAGTAAATGGTGGGA
SEQUENCE_INCLUDED_REGION=8,243
SEQUENCE_TARGET=104,52
P3_COMMENT=CL is GCATGGAATTTGATTCCAAA
P3_COMMENT=CR is TTGCAAGGAACAGAACCAACT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.830638
PRIMER_LEFT_0_PENALTY=1.596296
PRIMER_RIGHT_0_PENALTY=1.234342
PRIMER_LEFT_0_SEQUENCE=GCATGGAATTTGATTCCAAA
PRIMER_RIGHT_0_SEQUENCE=TTGCAAGGAACAGAACCAACT
PRIMER_LEFT_0=32,20
PRIMER_RIGHT_0=202,21
PRIMER_LEFT_0_TM=58.404
PRIMER_RIGHT_0_TM=59.766
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=66.7
PRIMER_PAIR_1_PENALTY=2.830638
PRIMER_LEFT_1_PENALTY=1.596296
PRIMER_RIGHT_1_PENALTY=1.234342
PRIMER_LEFT_1_SEQUENCE=GCATGGAATTTGATTCCAAA
PRIMER_RIGHT_1_SEQUENCE=AGTTGCAAGGAACAGAACCAA
PRIMER_LEFT_1=32,20
PRIMER_RIGHT_1=204,21
PRIMER_LEFT_1_TM=58.404
PRIMER_RIGHT_1_TM=59.766
PRIMER_LEFT_1_GC_PERCENT=35.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=173
PRIMER_PAIR_1_PRODUCT_TM=66.9
PRIMER_PAIR_2_PENALTY=2.847767
PRIMER_LEFT_2_PENALTY=1.596296
PRIMER_RIGHT_2_PENALTY=1.251471
PRIMER_LEFT_2_SEQUENCE=GCATGGAATTTGATTCCAAA
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGGAACAGAACCAAC
PRIMER_LEFT_2=32,20
PRIMER_RIGHT_2=202,20
PRIMER_LEFT_2_TM=58.404
PRIMER_RIGHT_2_TM=58.749
PRIMER_LEFT_2_GC_PERCENT=35.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=2.847767
PRIMER_LEFT_3_PENALTY=1.596296
PRIMER_RIGHT_3_PENALTY=1.251471
PRIMER_LEFT_3_SEQUENCE=GCATGGAATTTGATTCCAAA
PRIMER_RIGHT_3_SEQUENCE=GTTGCAAGGAACAGAACCAA
PRIMER_LEFT_3=32,20
PRIMER_RIGHT_3=203,20
PRIMER_LEFT_3_TM=58.404
PRIMER_RIGHT_3_TM=58.749
PRIMER_LEFT_3_GC_PERCENT=35.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=9.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=172
PRIMER_PAIR_3_PRODUCT_TM=66.9
PRIMER_PAIR_4_PENALTY=2.880019
PRIMER_LEFT_4_PENALTY=1.596296
PRIMER_RIGHT_4_PENALTY=1.283723
PRIMER_LEFT_4_SEQUENCE=GCATGGAATTTGATTCCAAA
PRIMER_RIGHT_4_SEQUENCE=TCAGTTGCAAGGAACAGAACC
PRIMER_LEFT_4=32,20
PRIMER_RIGHT_4=206,21
PRIMER_LEFT_4_TM=58.404
PRIMER_RIGHT_4_TM=60.284
PRIMER_LEFT_4_GC_PERCENT=35.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=9.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=175
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=07.RHAP68FC1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNAGGTAGATGATTAGAGAGAACAATCTATTTGGGGTGGATTATTGAGAATGAGAAAACCATTAGAAATGCCAGTAAAACTTCAAATTTGATTACATCTTCATCATCATCATATATTTGTGTGCATGCAAGTGCTCGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCAAAAATCAATAAAATATGCAGTAACTCTTTGTGACTTAGAATTTGGTNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,230
SEQUENCE_TARGET=157,40
P3_COMMENT=CL is TGGGGTGGATTATTGAGAATG
P3_COMMENT=CR is TGCATATTTTATTGATTTTTGGCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.893173
PRIMER_LEFT_0_PENALTY=1.369105
PRIMER_RIGHT_0_PENALTY=4.524067
PRIMER_LEFT_0_SEQUENCE=TGGGGTGGATTATTGAGAATG
PRIMER_RIGHT_0_SEQUENCE=TGCATATTTTATTGATTTTTGGCA
PRIMER_LEFT_0=48,21
PRIMER_RIGHT_0=221,24
PRIMER_LEFT_0_TM=59.631
PRIMER_RIGHT_0_TM=60.524
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=25.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=66.6
PRIMER_PAIR_1_PENALTY=6.215243
PRIMER_LEFT_1_PENALTY=1.369105
PRIMER_RIGHT_1_PENALTY=4.846138
PRIMER_LEFT_1_SEQUENCE=TGGGGTGGATTATTGAGAATG
PRIMER_RIGHT_1_SEQUENCE=GCATATTTTATTGATTTTTGGCA
PRIMER_LEFT_1=48,21
PRIMER_RIGHT_1=220,23
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=58.154
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=26.087
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=173
PRIMER_PAIR_1_PRODUCT_TM=66.6
PRIMER_PAIR_2_PENALTY=6.215243
PRIMER_LEFT_2_PENALTY=1.369105
PRIMER_RIGHT_2_PENALTY=4.846138
PRIMER_LEFT_2_SEQUENCE=TGGGGTGGATTATTGAGAATG
PRIMER_RIGHT_2_SEQUENCE=TGCATATTTTATTGATTTTTGGC
PRIMER_LEFT_2=48,21
PRIMER_RIGHT_2=221,23
PRIMER_LEFT_2_TM=59.631
PRIMER_RIGHT_2_TM=58.154
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=26.087
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=174
PRIMER_PAIR_2_PRODUCT_TM=66.6
PRIMER_PAIR_3_PENALTY=6.354873
PRIMER_LEFT_3_PENALTY=1.369105
PRIMER_RIGHT_3_PENALTY=4.985768
PRIMER_LEFT_3_SEQUENCE=TGGGGTGGATTATTGAGAATG
PRIMER_RIGHT_3_SEQUENCE=CTGCATATTTTATTGATTTTTGGC
PRIMER_LEFT_3=48,21
PRIMER_RIGHT_3=222,24
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=59.014
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=175
PRIMER_PAIR_3_PRODUCT_TM=66.8
PRIMER_PAIR_4_PENALTY=6.586452
PRIMER_LEFT_4_PENALTY=1.740315
PRIMER_RIGHT_4_PENALTY=4.846138
PRIMER_LEFT_4_SEQUENCE=TTTGGGGTGGATTATTGAGAA
PRIMER_RIGHT_4_SEQUENCE=GCATATTTTATTGATTTTTGGCA
PRIMER_LEFT_4=46,21
PRIMER_RIGHT_4=220,23
PRIMER_LEFT_4_TM=59.260
PRIMER_RIGHT_4_TM=58.154
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=26.087
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=175
PRIMER_PAIR_4_PRODUCT_TM=66.5
=
SEQUENCE_ID=08.RHAP68FC2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACATGCATAACCCATTCCTGAATCCATCTGCTGTCAAGCACACAACCCCGTGGCAGAAGCCTAGGGACCAGACAGGAAGCTTCTCCTGAGCTTCCCTGTGGCTGTGGCTAGCAGGAGGCCAGAAGGTCAGCATGGTGGTCCTGTGGGGGAGACACTGCAACCCAGCTAAGCTTCAGCTTGACTCCTGTTNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,223
SEQUENCE_TARGET=0,32
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=09.RHAP68FC3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGCATACCTTGTCATTCATGTAGAAGTTTGAAGATAACTTGTGAAGTCAGTTCTACTCTTTTACAATGTGGTTTCTAGGTTTCAAACTTAGGTTTTTAGGCTCAGCAACAAGTCCCTTTACTTCCTAAACCATCATACCAGGACCTGTAAAAGAATTGTCTTTCTTCCTGTTTAATTTAAAAAAACATTATTTTATTTCTGTN
SEQUENCE_INCLUDED_REGION=9,241
SEQUENCE_TARGET=9,36
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=11.RHAP68FD2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTATGCACACACACATGCACCATTATGTTTAACTTTATATATTTGTAAATATTGGTTATATCCTGTGAAGAACGTTTATTGCTAAACACTAAAACATAGAAAAAACAAATGAAGTTTGTTTAGATAATTAAGATGAGAAACTCTTTTGGGTAAGATAGGGTGATGGATTCAGGTAAAATGGGAAGCAGGAGTAATCTGG
SEQUENCE_INCLUDED_REGION=0,231
SEQUENCE_TARGET=0,28
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=19.RHAP68FG1.seq
SEQUENCE_TEMPLATE=NNNNNNATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAATAATCTGANTGTGTGTGTGAATCNTCTGATGATAATCTANTGNAGATAGTGATGATGTANTACTGAAGATGTGTNATATANACTGATA
SEQUENCE_INCLUDED_REGION=6,257
SEQUENCE_TARGET=10,102
SEQUENCE_TARGET=118,52
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=20.RHAP68FG2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNATCTGGTTCCAATGGATACCCATAATGCAAAATATGCCNNNNNNNNNNNNNCCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGCTCTAGGGGATCTTGCCTTTTAAAGTCAAGAGTTTAGTTGGGGGATTCAGTCTTCACTCACCAGAGGACACCATACAAATAAACTCTTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=16,208
SEQUENCE_TARGET=54,13
SEQUENCE_TARGET=72,52
P3_COMMENT=CL is TGGTTCCAATGGATACCCATA
P3_COMMENT=CR is TGAAGACTGAATCCCCCAAC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.210646
PRIMER_LEFT_0_PENALTY=1.113753
PRIMER_RIGHT_0_PENALTY=0.096893
PRIMER_LEFT_0_SEQUENCE=TGGTTCCAATGGATACCCATA
PRIMER_RIGHT_0_SEQUENCE=TGAAGACTGAATCCCCCAAC
PRIMER_LEFT_0=19,21
PRIMER_RIGHT_0=187,20
PRIMER_LEFT_0_TM=59.886
PRIMER_RIGHT_0_TM=59.903
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=169
PRIMER_PAIR_0_PRODUCT_TM=68.2
PRIMER_PAIR_1_PENALTY=1.210646
PRIMER_LEFT_1_PENALTY=1.113753
PRIMER_RIGHT_1_PENALTY=0.096893
PRIMER_LEFT_1_SEQUENCE=TGGTTCCAATGGATACCCATA
PRIMER_RIGHT_1_SEQUENCE=GTGAAGACTGAATCCCCCAA
PRIMER_LEFT_1=19,21
PRIMER_RIGHT_1=188,20
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=59.903
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=170
PRIMER_PAIR_1_PRODUCT_TM=68.4
PRIMER_PAIR_2_PENALTY=1.302988
PRIMER_LEFT_2_PENALTY=1.113753
PRIMER_RIGHT_2_PENALTY=0.189235
PRIMER_LEFT_2_SEQUENCE=TGGTTCCAATGGATACCCATA
PRIMER_RIGHT_2_SEQUENCE=AAGGCAAGATCCCCTAGAGC
PRIMER_LEFT_2=19,21
PRIMER_RIGHT_2=150,20
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.811
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=132
PRIMER_PAIR_2_PRODUCT_TM=66.2
PRIMER_PAIR_3_PENALTY=1.506016
PRIMER_LEFT_3_PENALTY=1.409123
PRIMER_RIGHT_3_PENALTY=0.096893
PRIMER_LEFT_3_SEQUENCE=CCAATGGATACCCATAATGCA
PRIMER_RIGHT_3_SEQUENCE=TGAAGACTGAATCCCCCAAC
PRIMER_LEFT_3=24,21
PRIMER_RIGHT_3=187,20
PRIMER_LEFT_3_TM=60.409
PRIMER_RIGHT_3_TM=59.903
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=164
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=1.506016
PRIMER_LEFT_4_PENALTY=1.409123
PRIMER_RIGHT_4_PENALTY=0.096893
PRIMER_LEFT_4_SEQUENCE=CCAATGGATACCCATAATGCA
PRIMER_RIGHT_4_SEQUENCE=GTGAAGACTGAATCCCCCAA
PRIMER_LEFT_4=24,21
PRIMER_RIGHT_4=188,20
PRIMER_LEFT_4_TM=60.409
PRIMER_RIGHT_4_TM=59.903
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=165
PRIMER_PAIR_4_PRODUCT_TM=68.2
=
SEQUENCE_ID=25.RHAP68FA4.seq
SEQUENCE_TEMPLATE=NNNNNNNNACGAANNNNNNNNNNNNNNNNNNNNGATGATGANNNNNNNNNNNNNNNNNNNNCATGCTCACTTCTGGGGGCTCAGGATGTCATTCCTTAGGCAATGATCATCTATGNGNTTTTGTTTTTGTTTGATAACCATGTATTCTTACCTAGACCTGTATCCCATGAAAGACATCNTGAGAGAGATCTACAAAAATGTCAACATACATATTACAAAACATAGATAGGNACTCATGAGAAATATATAAANCATTGATAGACACCATACAAATAGACTATAGACAANAGNTANAATAGAGAAGNCACAATGCGACACACACNAT
SEQUENCE_INCLUDED_REGION=8,317
SEQUENCE_TARGET=13,20
SEQUENCE_TARGET=41,20
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=27.RHAP68FA6.seq
SEQUENCE_TEMPLATE=TNCCACTACTATGTCTATGGAGAACACAACAAATTGAAATAGATCAGAGCTAAGGTTCAGATTGTTCTGATCTGGAATTTGGCATCAGTGTTGAAGTTTTGAATGGCTTAGAATACCCAAGTAAATTTTTAACTAAGAAACTGTTTCAGAAGTTCTCCTAAGATCTGNNNNNNNNNNNNNNNNNNNNGATTGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGATATTTCCT
SEQUENCE_INCLUDED_REGION=0,247
SEQUENCE_TARGET=167,20
SEQUENCE_TARGET=195,38
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=28.RHAP68FB4.seq
SEQUENCE_TEMPLATE=NNNNNNNACACACACACACATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCTTCACTCAGGCATCTGTTCTTTGTCAATTTTCCTCCTGAAGGGAAAAGACTTAGCACTATTACTCCTTCTGTTTTAGGAGAGATTTTTATTTTTACTT
SEQUENCE_INCLUDED_REGION=7,246
SEQUENCE_TARGET=24,72
SEQUENCE_TARGET=104,46
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=32.RHAP68FC5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNTCTTTATNGCCTGTCTAAGACAGAAACAGTAAAGCTGAAACCTAAAGTCAGGGTAGTCAGGCCTAGAAAGGTGGGAAGCAGACAGTTCAGTAATTATCCATGTGCAGGGAACAGAAGAAATACATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCNCCGCAGNAGNGTGTGTGTGTGGNNNNGTGTGTGGTNATGT
SEQUENCE_INCLUDED_REGION=24,244
SEQUENCE_TARGET=156,29
SEQUENCE_TARGET=193,30
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=33.RHAP68FC6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACGCTTTATTAGTTCCCGAATATGTTCTATTTTCTTTTTGGTATGAGGATTCCTTCAGTTCTCTTCTGTAGTGAAGGTTTACCACTCACACACTCCTACAGCATATTTTTTATAATGGAAAGTTATTTGAAAAACAGATTTATAATTTTTATTTACCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=16,243
SEQUENCE_TARGET=176,46
SEQUENCE_TARGET=223,36
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=35.RHAP68FD5.seq
SEQUENCE_TEMPLATE=NNNNNNACGGTGNCCTTGCCTGAAAATAAAAACAGTAAAGATGGAAATGTAGTTTGACAGTCTGCTTGTCCAATGAGCTTATTAGCTTAAGTTTTATCCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGATCAGGGAGAAAGATGTTCAGGGAGAGGGAGGGAGAGAGAAAGCAGGAAAAACGGTGTTCTGATACAATGCTCCTCTCCCCNTCTCTTT
SEQUENCE_INCLUDED_REGION=6,250
SEQUENCE_TARGET=103,59
P3_COMMENT=CL is TGACAGTCTGCTTGTCCAATG
P3_COMMENT=CR is TTCTCTCTCCCTCCCTCTCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.228790
PRIMER_LEFT_0_PENALTY=1.111304
PRIMER_RIGHT_0_PENALTY=0.117486
PRIMER_LEFT_0_SEQUENCE=TGACAGTCTGCTTGTCCAATG
PRIMER_RIGHT_0_SEQUENCE=TTCTCTCTCCCTCCCTCTCC
PRIMER_LEFT_0=54,21
PRIMER_RIGHT_0=207,20
PRIMER_LEFT_0_TM=59.889
PRIMER_RIGHT_0_TM=59.883
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=154
PRIMER_PAIR_0_PRODUCT_TM=67.5
PRIMER_PAIR_1_PENALTY=1.520031
PRIMER_LEFT_1_PENALTY=1.402545
PRIMER_RIGHT_1_PENALTY=0.117486
PRIMER_LEFT_1_SEQUENCE=TCTGCTTGTCCAATGAGCTT
PRIMER_RIGHT_1_SEQUENCE=TTCTCTCTCCCTCCCTCTCC
PRIMER_LEFT_1=60,20
PRIMER_RIGHT_1=207,20
PRIMER_LEFT_1_TM=58.597
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=148
PRIMER_PAIR_1_PRODUCT_TM=66.9
PRIMER_PAIR_2_PENALTY=1.579390
PRIMER_LEFT_2_PENALTY=1.111304
PRIMER_RIGHT_2_PENALTY=0.468086
PRIMER_LEFT_2_SEQUENCE=TGACAGTCTGCTTGTCCAATG
PRIMER_RIGHT_2_SEQUENCE=TGCTTTCTCTCTCCCTCCCT
PRIMER_LEFT_2=54,21
PRIMER_RIGHT_2=211,20
PRIMER_LEFT_2_TM=59.889
PRIMER_RIGHT_2_TM=60.468
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=67.8
PRIMER_PAIR_3_PENALTY=1.579390
PRIMER_LEFT_3_PENALTY=1.111304
PRIMER_RIGHT_3_PENALTY=0.468086
PRIMER_LEFT_3_SEQUENCE=TGACAGTCTGCTTGTCCAATG
PRIMER_RIGHT_3_SEQUENCE=CTGCTTTCTCTCTCCCTCCC
PRIMER_LEFT_3=54,21
PRIMER_RIGHT_3=212,20
PRIMER_LEFT_3_TM=59.889
PRIMER_RIGHT_3_TM=60.468
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=159
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=1.579390
PRIMER_LEFT_4_PENALTY=1.111304
PRIMER_RIGHT_4_PENALTY=0.468086
PRIMER_LEFT_4_SEQUENCE=TGACAGTCTGCTTGTCCAATG
PRIMER_RIGHT_4_SEQUENCE=CCTGCTTTCTCTCTCCCTCC
PRIMER_LEFT_4=54,21
PRIMER_RIGHT_4=213,20
PRIMER_LEFT_4_TM=59.889
PRIMER_RIGHT_4_TM=60.468
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=160
PRIMER_PAIR_4_PRODUCT_TM=68.2
=
SEQUENCE_ID=36.RHAP68FD6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNACATATACATACATACATACATACATNNNNNNNNNNNNNNATACATATACACATATACATACACACACATACATATACACATATACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGTTAGGGAAATAAGATTCAAGGAAGAAGCGATATTATTTTCATATCATAAAACTGGAACAGGCTTAAACCAATAGAGAAATTCAAGTGTCTCTGATTCTAAAGTTCAGTCTT
SEQUENCE_INCLUDED_REGION=15,249
SEQUENCE_TARGET=41,14
SEQUENCE_TARGET=111,38
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=41.RHAP68FF5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNAATATATGCACACACATATGCGAAACCCATTTTCACTTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNTTTGACTACACAGGTATTTGTCAAATTAAGAATGGCAAAATAAAATGTGATGACCTGCATTTGATTAACAGTTAAGGGAACAATCTATTCTTACAGAGGACTCATATTTTTTTCCATGCTCCAATACAGAGTGGAGCATA
SEQUENCE_INCLUDED_REGION=18,242
SEQUENCE_TARGET=60,43
SEQUENCE_TARGET=104,16
P3_COMMENT=CL is GCACACACATATGCGAAACC
P3_COMMENT=CR is TCAAATGCAGGTCATCACATT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.012201
PRIMER_LEFT_0_PENALTY=0.002648
PRIMER_RIGHT_0_PENALTY=2.009552
PRIMER_LEFT_0_SEQUENCE=GCACACACATATGCGAAACC
PRIMER_RIGHT_0_SEQUENCE=TCAAATGCAGGTCATCACATT
PRIMER_LEFT_0=25,20
PRIMER_RIGHT_0=183,21
PRIMER_LEFT_0_TM=60.003
PRIMER_RIGHT_0_TM=58.990
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=159
PRIMER_PAIR_0_PRODUCT_TM=65.4
PRIMER_PAIR_1_PENALTY=2.012201
PRIMER_LEFT_1_PENALTY=0.002648
PRIMER_RIGHT_1_PENALTY=2.009552
PRIMER_LEFT_1_SEQUENCE=GCACACACATATGCGAAACC
PRIMER_RIGHT_1_SEQUENCE=AATCAAATGCAGGTCATCACA
PRIMER_LEFT_1=25,20
PRIMER_RIGHT_1=185,21
PRIMER_LEFT_1_TM=60.003
PRIMER_RIGHT_1_TM=58.990
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=161
PRIMER_PAIR_1_PRODUCT_TM=65.3
PRIMER_PAIR_2_PENALTY=2.135505
PRIMER_LEFT_2_PENALTY=0.002648
PRIMER_RIGHT_2_PENALTY=2.132857
PRIMER_LEFT_2_SEQUENCE=GCACACACATATGCGAAACC
PRIMER_RIGHT_2_SEQUENCE=CAAATGCAGGTCATCACATTTT
PRIMER_LEFT_2=25,20
PRIMER_RIGHT_2=182,22
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=65.4
PRIMER_PAIR_3_PENALTY=2.162551
PRIMER_LEFT_3_PENALTY=0.152999
PRIMER_RIGHT_3_PENALTY=2.009552
PRIMER_LEFT_3_SEQUENCE=ACACACATATGCGAAACCCA
PRIMER_RIGHT_3_SEQUENCE=TCAAATGCAGGTCATCACATT
PRIMER_LEFT_3=27,20
PRIMER_RIGHT_3=183,21
PRIMER_LEFT_3_TM=59.847
PRIMER_RIGHT_3_TM=58.990
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=157
PRIMER_PAIR_3_PRODUCT_TM=65.0
PRIMER_PAIR_4_PENALTY=2.162551
PRIMER_LEFT_4_PENALTY=0.152999
PRIMER_RIGHT_4_PENALTY=2.009552
PRIMER_LEFT_4_SEQUENCE=ACACACATATGCGAAACCCA
PRIMER_RIGHT_4_SEQUENCE=AATCAAATGCAGGTCATCACA
PRIMER_LEFT_4=27,20
PRIMER_RIGHT_4=185,21
PRIMER_LEFT_4_TM=59.847
PRIMER_RIGHT_4_TM=58.990
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=159
PRIMER_PAIR_4_PRODUCT_TM=64.9
=
SEQUENCE_ID=42.RHAP68FF6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACTCANGATGCTGATCCAATTATCTACAACCTTATAAACATATTTGATAAGTATTCTGAAAAGGCAATCATGAATACACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGTTCCACTGAAAATTTGGAATGAGTTTTTCATTCCTCAAGTTCTTTAGAAATAAAAAATTCTGCTAAGAAAATCATTAGCTCAAAAGACAATGAAAGTAACAAATTATTTAGCTCTTTCAAG
SEQUENCE_INCLUDED_REGION=16,245
SEQUENCE_TARGET=98,38
P3_COMMENT=CL is TGCTGATCCAATTATCTACAACC
P3_COMMENT=CR is TCCAAATTTTCAGTGGAACATG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.010692
PRIMER_LEFT_0_PENALTY=4.849609
PRIMER_RIGHT_0_PENALTY=2.161083
PRIMER_LEFT_0_SEQUENCE=TGCTGATCCAATTATCTACAACC
PRIMER_RIGHT_0_SEQUENCE=TCCAAATTTTCAGTGGAACATG
PRIMER_LEFT_0=24,23
PRIMER_RIGHT_0=157,22
PRIMER_LEFT_0_TM=58.150
PRIMER_RIGHT_0_TM=59.839
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=134
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=7.079039
PRIMER_LEFT_1_PENALTY=4.917956
PRIMER_RIGHT_1_PENALTY=2.161083
PRIMER_LEFT_1_SEQUENCE=TGCTGATCCAATTATCTACAACCT
PRIMER_RIGHT_1_SEQUENCE=TCCAAATTTTCAGTGGAACATG
PRIMER_LEFT_1=24,24
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=59.082
PRIMER_RIGHT_1_TM=59.839
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=134
PRIMER_PAIR_1_PRODUCT_TM=64.6
PRIMER_PAIR_2_PENALTY=7.275237
PRIMER_LEFT_2_PENALTY=5.114154
PRIMER_RIGHT_2_PENALTY=2.161083
PRIMER_LEFT_2_SEQUENCE=GATGCTGATCCAATTATCTACAACC
PRIMER_RIGHT_2_SEQUENCE=TCCAAATTTTCAGTGGAACATG
PRIMER_LEFT_2=22,25
PRIMER_RIGHT_2=157,22
PRIMER_LEFT_2_TM=60.114
PRIMER_RIGHT_2_TM=59.839
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=136
PRIMER_PAIR_2_PRODUCT_TM=64.8
PRIMER_PAIR_3_PENALTY=7.418295
PRIMER_LEFT_3_PENALTY=5.257212
PRIMER_RIGHT_3_PENALTY=2.161083
PRIMER_LEFT_3_SEQUENCE=TGCTGATCCAATTATCTACAACCTT
PRIMER_RIGHT_3_SEQUENCE=TCCAAATTTTCAGTGGAACATG
PRIMER_LEFT_3=24,25
PRIMER_RIGHT_3=157,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=59.839
PRIMER_LEFT_3_GC_PERCENT=36.000
PRIMER_RIGHT_3_GC_PERCENT=36.364
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=134
PRIMER_PAIR_3_PRODUCT_TM=64.6
PRIMER_PAIR_4_PENALTY=7.420731
PRIMER_LEFT_4_PENALTY=4.849609
PRIMER_RIGHT_4_PENALTY=2.571122
PRIMER_LEFT_4_SEQUENCE=TGCTGATCCAATTATCTACAACC
PRIMER_RIGHT_4_SEQUENCE=TCATTCCAAATTTTCAGTGGAA
PRIMER_LEFT_4=24,23
PRIMER_RIGHT_4=161,22
PRIMER_LEFT_4_TM=58.150
PRIMER_RIGHT_4_TM=59.429
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=31.818
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=64.8
=
SEQUENCE_ID=45.RHAP68FG6.seq
SEQUENCE_TEMPLATE=NNNNNNNACANNATACANGTTGCTACTAGGTCCTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATATGTATATATGTTCAANNNNNNNNNNNNNNNNNNNNNNNCTTTTAGCTTATTTTTATGTGACTCCTGGCTATGAAAAGGAGCGGGTGCTGACACTGGTTGTAGCTCTTGTGACACTTTTCCTCCTGTTGGGGTT
SEQUENCE_INCLUDED_REGION=7,240
SEQUENCE_TARGET=39,67
SEQUENCE_TARGET=129,23
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=48.RHAP68FH6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTTATGTATTTAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATCATGTGCTGTGTTATAACAACAAAGAGTAAATAAAACGGCCATGAATTTGAGAGAGAGCATGGAGGGTTTATGGGAGGGAGGAAAGGAAAAGCTCATATGATATAATTTTAGAAAGAGAAAAAAAATCAGGAAACACATGTATTCAGTTTTCCCAAAGACTCTGAAGCGTAACACTAGTTTATGCAGCAGAATG
SEQUENCE_INCLUDED_REGION=17,246
SEQUENCE_TARGET=31,32
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=01.RHAP69FA7.seq
SEQUENCE_TEMPLATE=TGNGNTACGTTNCNCTGNTTTGGCCGTTTTTTGCCNATCCGGNGTCACACTGTTTGAGCCACGGATATCCCAGTCAGCTGTTTCCTACCTTTCCACATAAGGGAGAAATCTTAGTCCTTATGTGGGGCCAAGAATACATGGGGTGGGGGTGGGGGCATAAACAGGTTTTGCTTCTTGCGCTATATGGAATGGAAAGAACCAGACTACAGAGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCTANACAAATTGGGGAGGGGGGTNTAGAAACCCAGGGCAAGCAGATCCCAGGAGCACCGAGNAGGC
SEQUENCE_INCLUDED_REGION=0,326
SEQUENCE_TARGET=217,40
P3_COMMENT=CL is AGGTTTTGCTTCTTGCGCTA
P3_COMMENT=CR is GTGCTCCTGGGATCTGCTT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.516964
PRIMER_LEFT_0_PENALTY=0.151911
PRIMER_RIGHT_0_PENALTY=1.365052
PRIMER_LEFT_0_SEQUENCE=AGGTTTTGCTTCTTGCGCTA
PRIMER_RIGHT_0_SEQUENCE=GTGCTCCTGGGATCTGCTT
PRIMER_LEFT_0=162,20
PRIMER_RIGHT_0=316,19
PRIMER_LEFT_0_TM=60.152
PRIMER_RIGHT_0_TM=60.365
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=155
PRIMER_PAIR_0_PRODUCT_TM=71.4
PRIMER_PAIR_1_PENALTY=1.784778
PRIMER_LEFT_1_PENALTY=0.419726
PRIMER_RIGHT_1_PENALTY=1.365052
PRIMER_LEFT_1_SEQUENCE=AACAGGTTTTGCTTCTTGCG
PRIMER_RIGHT_1_SEQUENCE=GTGCTCCTGGGATCTGCTT
PRIMER_LEFT_1=159,20
PRIMER_RIGHT_1=316,19
PRIMER_LEFT_1_TM=60.420
PRIMER_RIGHT_1_TM=60.365
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=158
PRIMER_PAIR_1_PRODUCT_TM=71.4
PRIMER_PAIR_2_PENALTY=1.798166
PRIMER_LEFT_2_PENALTY=0.433114
PRIMER_RIGHT_2_PENALTY=1.365052
PRIMER_LEFT_2_SEQUENCE=TGCTTCTTGCGCTATATGGA
PRIMER_RIGHT_2_SEQUENCE=GTGCTCCTGGGATCTGCTT
PRIMER_LEFT_2=168,20
PRIMER_RIGHT_2=316,19
PRIMER_LEFT_2_TM=59.567
PRIMER_RIGHT_2_TM=60.365
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=149
PRIMER_PAIR_2_PRODUCT_TM=71.3
PRIMER_PAIR_3_PENALTY=1.863941
PRIMER_LEFT_3_PENALTY=0.498888
PRIMER_RIGHT_3_PENALTY=1.365052
PRIMER_LEFT_3_SEQUENCE=GGTTTTGCTTCTTGCGCTAT
PRIMER_RIGHT_3_SEQUENCE=GTGCTCCTGGGATCTGCTT
PRIMER_LEFT_3=163,20
PRIMER_RIGHT_3=316,19
PRIMER_LEFT_3_TM=59.501
PRIMER_RIGHT_3_TM=60.365
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=154
PRIMER_PAIR_3_PRODUCT_TM=71.4
PRIMER_PAIR_4_PENALTY=2.239938
PRIMER_LEFT_4_PENALTY=0.874885
PRIMER_RIGHT_4_PENALTY=1.365052
PRIMER_LEFT_4_SEQUENCE=TGCGCTATATGGAATGGAAA
PRIMER_RIGHT_4_SEQUENCE=GTGCTCCTGGGATCTGCTT
PRIMER_LEFT_4=175,20
PRIMER_RIGHT_4=316,19
PRIMER_LEFT_4_TM=59.125
PRIMER_RIGHT_4_TM=60.365
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=142
PRIMER_PAIR_4_PRODUCT_TM=71.0
=
SEQUENCE_ID=03.RHAP69FA9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNACGTTCTCCTGTAGGACAAAGACTTTCTGTGGTTACGACGGATATTTCTCAGACTTTATCATTAAAAAGAACTGACATACAGCTGCGGCTGTAGTTCACATGTAGGATATTTGCTGAGCATGGATGAAACTCTGGGTTTGAGTCCCACCATCAGAAAAATGTGCTTCCCATTTATATGGAGAGTTATTAAATTTGTTCTCACAGTTTGATCCATTTCTCCTCAATATAAAGATCTATGAAGGAATAAGAAGGGAGAGTTCTGCTTTGATGTTAATTAGCAAGAAAGACGACATAGCAAGAAACAGAGCTGAGTCCAGATTCCAGCACATGTGCGCTNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=20,350
SEQUENCE_TARGET=356,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=04.RHAP69FB1.seq
SEQUENCE_TEMPLATE=CNGNATTATNTCCANAAANAAATTATTTNATTGCAACANAAAACATTACATGTCAGCCTTAAAAAGCAATTAAATCAAAAAAACAAAAATACCAATTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCATCTCAAGACTCTGTTTCTGTTGTGGGGCCTGTGGGGTCTGGAGCTCCTTTGAAACGGAGACTTGGAAATTGCTCACCTGGCTGAGCTTTGGCCCCTCAGAGGTGGGACTTTCTTTACTAACAATAGGAAGCTGGAGAAGCTGTCAAGTGCGAATGGAGGAGACCTGCTCAGAAAGGGAGGGGAGCTGTGAGAATTTGGTTTCTCTCAGTATTGGGGTGGGGGTGGGGTCTGGATTTACTTGTGCCTAAGATTCCTTTAAAACTCTGACCCGATGCA
SEQUENCE_INCLUDED_REGION=0,435
SEQUENCE_TARGET=100,52
P3_COMMENT=CL is CATGTCAGCCTTAAAAAGCAA
P3_COMMENT=CR is TCTCCGTTTCAAAGGAGCTC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.339631
PRIMER_LEFT_0_PENALTY=2.889802
PRIMER_RIGHT_0_PENALTY=0.449829
PRIMER_LEFT_0_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
PRIMER_RIGHT_0_SEQUENCE=TCTCCGTTTCAAAGGAGCTC
PRIMER_LEFT_0=48,21
PRIMER_RIGHT_0=218,20
PRIMER_LEFT_0_TM=58.110
PRIMER_RIGHT_0_TM=59.550
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=67.4
PRIMER_PAIR_1_PENALTY=3.397323
PRIMER_LEFT_1_PENALTY=2.889802
PRIMER_RIGHT_1_PENALTY=0.507521
PRIMER_LEFT_1_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
PRIMER_RIGHT_1_SEQUENCE=CGTTTCAAAGGAGCTCCAGA
PRIMER_LEFT_1=48,21
PRIMER_RIGHT_1=214,20
PRIMER_LEFT_1_TM=58.110
PRIMER_RIGHT_1_TM=60.508
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=167
PRIMER_PAIR_1_PRODUCT_TM=67.1
PRIMER_PAIR_2_PENALTY=3.484515
PRIMER_LEFT_2_PENALTY=2.889802
PRIMER_RIGHT_2_PENALTY=0.594713
PRIMER_LEFT_2_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
PRIMER_RIGHT_2_SEQUENCE=TTTCAAAGGAGCTCCAGACC
PRIMER_LEFT_2=48,21
PRIMER_RIGHT_2=212,20
PRIMER_LEFT_2_TM=58.110
PRIMER_RIGHT_2_TM=59.405
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=165
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=3.504849
PRIMER_LEFT_3_PENALTY=3.055020
PRIMER_RIGHT_3_PENALTY=0.449829
PRIMER_LEFT_3_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA
PRIMER_RIGHT_3_SEQUENCE=TCTCCGTTTCAAAGGAGCTC
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=218,20
PRIMER_LEFT_3_TM=58.945
PRIMER_RIGHT_3_TM=59.550
PRIMER_LEFT_3_GC_PERCENT=36.364
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=172
PRIMER_PAIR_3_PRODUCT_TM=67.4
PRIMER_PAIR_4_PENALTY=3.562541
PRIMER_LEFT_4_PENALTY=3.055020
PRIMER_RIGHT_4_PENALTY=0.507521
PRIMER_LEFT_4_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA
PRIMER_RIGHT_4_SEQUENCE=CGTTTCAAAGGAGCTCCAGA
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=214,20
PRIMER_LEFT_4_TM=58.945
PRIMER_RIGHT_4_TM=60.508
PRIMER_LEFT_4_GC_PERCENT=36.364
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=168
PRIMER_PAIR_4_PRODUCT_TM=67.1
=
SEQUENCE_ID=09.RHAP69FC9.seq
SEQUENCE_TEMPLATE=NNNNCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGTTAGTTAAGAAGACAGTTAAAGATGGATTGAAAGTAAGCATTGTGGTTTGTGGTTAGACCTTGTCAAACTGTTATCCAGATAAGTCATAAAATATTTCAATGGTTCGTTTGAACCGATATAAATTCTTTCTAAAGAAATACTATAGTATTGCATTAGCAATATGGCACTGTGATAAATTAGAAAAGAGATTAAGTGTTCACACCTCGGATTGTTGTTTTCTCACTAAGCAATTATGTTAATTCAGAACTATGCAAAGCAAGGAGGAAAGAAAATAAGAAGCCACAAAGA
SEQUENCE_INCLUDED_REGION=4,350
SEQUENCE_TARGET=9,52
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=10.RHAP69FD1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACTTTTTAAAAAACCAAAAATCAGGGGAGATGGCTCACACAGCAAAGGGCCAGATGTGTGAGCTTAAGGTTCGAGGCACTCCTGTCCAGTGTCTTCTGAGATTATTACAGTTCTTGGGAAGTAAAGACATGAAGATGACTGGACCAAACTTGTCAGCCCACTCTAGCAAAATAAGACTGCTAGGTTCAATGAAAAACCCTATCTAAAAAAATAAGGAAAGTGATGAAGAGTGAAGACACCTGATGTTAGCTTTTGACCTCCAAGTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCATACATACATGCACACACATGTGCATGCACATACAAACAATCATGCACACAGACATAAGCTCTAGAAATTACCATCAGTAATGCAACAGACTTTTTAAAGACTGGTATTCTAATAGTAAGTNNNNNNNNN
SEQUENCE_INCLUDED_REGION=16,443
SEQUENCE_TARGET=284,50
P3_COMMENT=CL is TGCTAGGTTCAATGAAAAACCC
P3_COMMENT=CR is ATGCACATGTGTGTGCATGT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.901866
PRIMER_LEFT_0_PENALTY=2.343977
PRIMER_RIGHT_0_PENALTY=0.557889
PRIMER_LEFT_0_SEQUENCE=TGCTAGGTTCAATGAAAAACCC
PRIMER_RIGHT_0_SEQUENCE=ATGCACATGTGTGTGCATGT
PRIMER_LEFT_0=193,22
PRIMER_RIGHT_0=363,20
PRIMER_LEFT_0_TM=60.344
PRIMER_RIGHT_0_TM=59.442
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=67.2
PRIMER_PAIR_1_PENALTY=3.230093
PRIMER_LEFT_1_PENALTY=2.343977
PRIMER_RIGHT_1_PENALTY=0.886116
PRIMER_LEFT_1_SEQUENCE=TGCTAGGTTCAATGAAAAACCC
PRIMER_RIGHT_1_SEQUENCE=TGCACATGTGTGTGCATGTA
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=362,20
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=59.114
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=170
PRIMER_PAIR_1_PRODUCT_TM=67.2
PRIMER_PAIR_2_PENALTY=3.658858
PRIMER_LEFT_2_PENALTY=3.100969
PRIMER_RIGHT_2_PENALTY=0.557889
PRIMER_LEFT_2_SEQUENCE=TGATGAAGAGTGAAGACACCTGA
PRIMER_RIGHT_2_SEQUENCE=ATGCACATGTGTGTGCATGT
PRIMER_LEFT_2=236,23
PRIMER_RIGHT_2=363,20
PRIMER_LEFT_2_TM=59.899
PRIMER_RIGHT_2_TM=59.442
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=128
PRIMER_PAIR_2_PRODUCT_TM=65.5
PRIMER_PAIR_3_PENALTY=3.823442
PRIMER_LEFT_3_PENALTY=3.265553
PRIMER_RIGHT_3_PENALTY=0.557889
PRIMER_LEFT_3_SEQUENCE=GCTAGGTTCAATGAAAAACCC
PRIMER_RIGHT_3_SEQUENCE=ATGCACATGTGTGTGCATGT
PRIMER_LEFT_3=194,21
PRIMER_RIGHT_3=363,20
PRIMER_LEFT_3_TM=57.734
PRIMER_RIGHT_3_TM=59.442
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=170
PRIMER_PAIR_3_PRODUCT_TM=67.2
PRIMER_PAIR_4_PENALTY=3.863755
PRIMER_LEFT_4_PENALTY=3.305865
PRIMER_RIGHT_4_PENALTY=0.557889
PRIMER_LEFT_4_SEQUENCE=GCTAGGTTCAATGAAAAACCCT
PRIMER_RIGHT_4_SEQUENCE=ATGCACATGTGTGTGCATGT
PRIMER_LEFT_4=194,22
PRIMER_RIGHT_4=363,20
PRIMER_LEFT_4_TM=58.694
PRIMER_RIGHT_4_TM=59.442
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=170
PRIMER_PAIR_4_PRODUCT_TM=67.2
=
SEQUENCE_ID=13.RHAP69FE1.seq
SEQUENCE_TEMPLATE=AAACAAACATAAAGTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAGAAAAATGTTCCTGCAGTGATGTGGATGAAGGATAGGGATGGATTGAAGACGCCAGCAGGCTGGCTTTGTGAGAGTCCAAATAAAACCCTGACAGCAGAGGCATGAGAAGGATTCCAGAAATTTTTAGAACATCACAGCCCAGGCTTTGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,233
SEQUENCE_TARGET=20,56
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=15.RHAP69FE3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNACACAATTCATTTAAACCACATGCTAGAGTCTACCCTTACAAAAAAAGCAAAAAAGCAAAATATTATAACTTAATTTTTTGATTTCTCTCTTCTATATCATGTCCTACTCTCTCCTTTGTAGATACCATCTCTACCACCTGGTAACACAGCATTTCTTTTTTTCTCTTTCTACATGAATATATCTAGACGACTCAGACACTCATCTACATAAAGAGAAAAATAAGCCAAGACCCTCCCTCCCTCATATGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTTCCATGAGTGTGAAAATGTCCCTGTGGGGAAAATAGCCAGTAGTAGCCAGTATGCAAATTTAAAAAGAAAAGGTTCAGTTCTTCATTCTTCAATTCCTGTCTCACATCATCTTTGTTGCCTTAAGGACAATGAAAGAATCAAAATATCTGCTAGAAACTTTCCTTGGT
SEQUENCE_INCLUDED_REGION=17,446
SEQUENCE_TARGET=272,22
P3_COMMENT=CL is CCACCTGGTAACACAGCATTT
P3_COMMENT=CR is CCACAGGGACATTTTCACACT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.218449
PRIMER_LEFT_0_PENALTY=1.094209
PRIMER_RIGHT_0_PENALTY=1.124240
PRIMER_LEFT_0_SEQUENCE=CCACCTGGTAACACAGCATTT
PRIMER_RIGHT_0_SEQUENCE=CCACAGGGACATTTTCACACT
PRIMER_LEFT_0=151,21
PRIMER_RIGHT_0=321,21
PRIMER_LEFT_0_TM=59.906
PRIMER_RIGHT_0_TM=59.876
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=71.0
PRIMER_PAIR_1_PENALTY=2.251449
PRIMER_LEFT_1_PENALTY=1.094209
PRIMER_RIGHT_1_PENALTY=1.157240
PRIMER_LEFT_1_SEQUENCE=CCACCTGGTAACACAGCATTT
PRIMER_RIGHT_1_SEQUENCE=TTCCCCACAGGGACATTTT
PRIMER_LEFT_1=151,21
PRIMER_RIGHT_1=325,19
PRIMER_LEFT_1_TM=59.906
PRIMER_RIGHT_1_TM=60.157
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=47.368
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=71.2
PRIMER_PAIR_2_PENALTY=2.267158
PRIMER_LEFT_2_PENALTY=1.094209
PRIMER_RIGHT_2_PENALTY=1.172949
PRIMER_LEFT_2_SEQUENCE=CCACCTGGTAACACAGCATTT
PRIMER_RIGHT_2_SEQUENCE=CCACAGGGACATTTTCACAC
PRIMER_LEFT_2=151,21
PRIMER_RIGHT_2=321,20
PRIMER_LEFT_2_TM=59.906
PRIMER_RIGHT_2_TM=58.827
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=71.0
PRIMER_PAIR_3_PENALTY=2.319369
PRIMER_LEFT_3_PENALTY=1.094209
PRIMER_RIGHT_3_PENALTY=1.225160
PRIMER_LEFT_3_SEQUENCE=CCACCTGGTAACACAGCATTT
PRIMER_RIGHT_3_SEQUENCE=GGGACATTTTCACACTCATGG
PRIMER_LEFT_3=151,21
PRIMER_RIGHT_3=316,21
PRIMER_LEFT_3_TM=59.906
PRIMER_RIGHT_3_TM=60.225
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=166
PRIMER_PAIR_3_PRODUCT_TM=70.6
PRIMER_PAIR_4_PENALTY=2.369803
PRIMER_LEFT_4_PENALTY=0.110099
PRIMER_RIGHT_4_PENALTY=2.259704
PRIMER_LEFT_4_SEQUENCE=AAGACCCTCCCTCCCTCATA
PRIMER_RIGHT_4_SEQUENCE=AGGCAACAAAGATGATGTGAGA
PRIMER_LEFT_4=244,20
PRIMER_RIGHT_4=415,22
PRIMER_LEFT_4_TM=59.890
PRIMER_RIGHT_4_TM=59.740
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=71.2
=
SEQUENCE_ID=21.RHAP69FG3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCAGCCATTCACAGTGCCTTCTCTGTCTCCTGCAGTCAGTTCAGGATGTAAGCCCTCAGCTACTGCAATGATCCCCAAATGAGAAATGAAATGTCTTAGTGAACTTTTTTTTTCAAAAAGTTAGGTATGCTTAGTGGTAGGAAGATGGCTCATTTGATAAAATGCTTGCCCCACACTCAAGCGTGATGATCTGTGTTTGGTTCTCTTATACCTATGATTAATAACAAAACCAAGAGTGGGGAGATCCTGCCTATTATCCCAACACTAGGAAGGCACAAATAGGAAGATCCCTGAAGCCTACCAACCTACCGTAGTCATTGAGCTCCAGGTTCAGGGAGAGACTCAAAAAATAAGATAGAGAATTGTAGAGAAAAATGTAAGCCCTCAACCTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG
SEQUENCE_INCLUDED_REGION=31,446
SEQUENCE_TARGET=427,48
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=25.RHAP69FA4.seq
SEQUENCE_TEMPLATE=NNNNNNCCTATAATGTAGGCACTGTTCTGTTTTACAAGTGACACAATTATTTCCTTGGCAGGCACAGAGCCTGAGTGTCCCCTCCAATCCCCCACACCAGGGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCATCTCCTACAAGGTGTGTGTGAACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=6,175
SEQUENCE_TARGET=106,44
P3_COMMENT=CL is AATTATTTCCTTGGCAGGCA
P3_COMMENT=CR is CCACGTTCACACACACCTTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.554604
PRIMER_LEFT_0_PENALTY=0.453004
PRIMER_RIGHT_0_PENALTY=1.101601
PRIMER_LEFT_0_SEQUENCE=AATTATTTCCTTGGCAGGCA
PRIMER_RIGHT_0_SEQUENCE=CCACGTTCACACACACCTTG
PRIMER_LEFT_0=44,20
PRIMER_RIGHT_0=180,20
PRIMER_LEFT_0_TM=59.547
PRIMER_RIGHT_0_TM=61.102
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=137
PRIMER_PAIR_0_PRODUCT_TM=70.8
PRIMER_PAIR_1_PENALTY=1.554604
PRIMER_LEFT_1_PENALTY=0.453004
PRIMER_RIGHT_1_PENALTY=1.101601
PRIMER_LEFT_1_SEQUENCE=CAATTATTTCCTTGGCAGGC
PRIMER_RIGHT_1_SEQUENCE=CCACGTTCACACACACCTTG
PRIMER_LEFT_1=43,20
PRIMER_RIGHT_1=180,20
PRIMER_LEFT_1_TM=59.547
PRIMER_RIGHT_1_TM=61.102
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=138
PRIMER_PAIR_1_PRODUCT_TM=71.0
PRIMER_PAIR_2_PENALTY=2.055158
PRIMER_LEFT_2_PENALTY=0.953558
PRIMER_RIGHT_2_PENALTY=1.101601
PRIMER_LEFT_2_SEQUENCE=ATTATTTCCTTGGCAGGCAC
PRIMER_RIGHT_2_SEQUENCE=CCACGTTCACACACACCTTG
PRIMER_LEFT_2=45,20
PRIMER_RIGHT_2=180,20
PRIMER_LEFT_2_TM=59.046
PRIMER_RIGHT_2_TM=61.102
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=136
PRIMER_PAIR_2_PRODUCT_TM=70.9
PRIMER_PAIR_3_PENALTY=2.258495
PRIMER_LEFT_3_PENALTY=1.156894
PRIMER_RIGHT_3_PENALTY=1.101601
PRIMER_LEFT_3_SEQUENCE=TATTTCCTTGGCAGGCACAG
PRIMER_RIGHT_3_SEQUENCE=CCACGTTCACACACACCTTG
PRIMER_LEFT_3=47,20
PRIMER_RIGHT_3=180,20
PRIMER_LEFT_3_TM=61.157
PRIMER_RIGHT_3_TM=61.102
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=134
PRIMER_PAIR_3_PRODUCT_TM=71.0
PRIMER_PAIR_4_PENALTY=2.305889
PRIMER_LEFT_4_PENALTY=1.204289
PRIMER_RIGHT_4_PENALTY=1.101601
PRIMER_LEFT_4_SEQUENCE=TATTTCCTTGGCAGGCACA
PRIMER_RIGHT_4_SEQUENCE=CCACGTTCACACACACCTTG
PRIMER_LEFT_4=47,19
PRIMER_RIGHT_4=180,20
PRIMER_LEFT_4_TM=60.204
PRIMER_RIGHT_4_TM=61.102
PRIMER_LEFT_4_GC_PERCENT=47.368
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=134
PRIMER_PAIR_4_PRODUCT_TM=71.0
=
SEQUENCE_ID=26.RHAP69FA11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTTATTTACAGAGACTTTTAAGGGTGACAGAAGCCTCTGAGTTAAGCCAGTGGGCACTATGTGATTTTCACATCTCCCAGCAGCTCCAGCATTGCTGTGAACTAGGGAGTTGACCAGTCTTCAAGTGATGCTGCTCTCAGAAACATTAGTCAAGAGGGTAGTTTTAAATTGCTGTTTGATTGGTTNNNNNNNNNNNNNNNNNNNTNCCGNCNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAATTAGCTAATCTAGAAACTCACACCTTGTTAAAGTGCAGAGACTAAGTGACCAAGAAGAATGAGTCACAAAGGGGACATCC
SEQUENCE_INCLUDED_REGION=18,353
SEQUENCE_TARGET=204,18
SEQUENCE_TARGET=231,18
SEQUENCE_TARGET=250,34
P3_COMMENT=CL is TTTTAAATTGCTGTTTGATTGGTT
P3_COMMENT=CR is TCTGCACTTTAACAAGGTGTGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.477001
PRIMER_LEFT_0_PENALTY=4.960837
PRIMER_RIGHT_0_PENALTY=3.516163
PRIMER_LEFT_0_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
PRIMER_RIGHT_0_SEQUENCE=TCTGCACTTTAACAAGGTGTGA
PRIMER_LEFT_0=180,24
PRIMER_RIGHT_0=329,22
PRIMER_LEFT_0_TM=59.039
PRIMER_RIGHT_0_TM=58.484
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=150
PRIMER_PAIR_0_PRODUCT_TM=62.7
PRIMER_PAIR_1_PENALTY=8.486325
PRIMER_LEFT_1_PENALTY=4.960837
PRIMER_RIGHT_1_PENALTY=3.525488
PRIMER_LEFT_1_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
PRIMER_RIGHT_1_SEQUENCE=TCTGCACTTTAACAAGGTGTGAG
PRIMER_LEFT_1=180,24
PRIMER_RIGHT_1=329,23
PRIMER_LEFT_1_TM=59.039
PRIMER_RIGHT_1_TM=59.475
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=150
PRIMER_PAIR_1_PRODUCT_TM=62.7
PRIMER_PAIR_2_PENALTY=8.486325
PRIMER_LEFT_2_PENALTY=4.960837
PRIMER_RIGHT_2_PENALTY=3.525488
PRIMER_LEFT_2_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
PRIMER_RIGHT_2_SEQUENCE=CTCTGCACTTTAACAAGGTGTGA
PRIMER_LEFT_2=180,24
PRIMER_RIGHT_2=330,23
PRIMER_LEFT_2_TM=59.039
PRIMER_RIGHT_2_TM=59.475
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=151
PRIMER_PAIR_2_PRODUCT_TM=63.0
PRIMER_PAIR_3_PENALTY=8.486325
PRIMER_LEFT_3_PENALTY=4.960837
PRIMER_RIGHT_3_PENALTY=3.525488
PRIMER_LEFT_3_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
PRIMER_RIGHT_3_SEQUENCE=TCTCTGCACTTTAACAAGGTGTG
PRIMER_LEFT_3=180,24
PRIMER_RIGHT_3=331,23
PRIMER_LEFT_3_TM=59.039
PRIMER_RIGHT_3_TM=59.475
PRIMER_LEFT_3_GC_PERCENT=25.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=152
PRIMER_PAIR_3_PRODUCT_TM=63.0
PRIMER_PAIR_4_PENALTY=8.581082
PRIMER_LEFT_4_PENALTY=5.064919
PRIMER_RIGHT_4_PENALTY=3.516163
PRIMER_LEFT_4_SEQUENCE=TCAAGAGGGTAGTTTTAAATTGCTG
PRIMER_RIGHT_4_SEQUENCE=TCTGCACTTTAACAAGGTGTGA
PRIMER_LEFT_4=168,25
PRIMER_RIGHT_4=329,22
PRIMER_LEFT_4_TM=60.065
PRIMER_RIGHT_4_TM=58.484
PRIMER_LEFT_4_GC_PERCENT=36.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=162
PRIMER_PAIR_4_PRODUCT_TM=64.0
=
SEQUENCE_ID=27.RHAP69FA6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATACTTCCTTTCAATTTTTAGCACTTCNAGTCATACATAGGACATAGTCACACATTTCCATTATGCTATAAAATATTGTCAAAATTTAAATAAGTCATCATGGTTGAAGTTATTTCATTTGCTATCTCTAAGATTAGAGCATGCTCTAATATCTTCCTGGTTCCAGAAAATTCAGACCTGTTTTTATCTTATTAAATACCCTCAAGCTCAGCTCATTCTTGAACTGTTGAACTGCAGCCAAGGTGAAGACATTCACATACAAATTAAACATTTTATTTTGCTAATGTTTTTGTGACTGCTTACTACTCAGCAGTAGCTCCTCAAACCAAAAACATTTGATGAGCCATGACTTTGTCATAAATAAGAAAAC
SEQUENCE_INCLUDED_REGION=19,447
SEQUENCE_TARGET=23,69
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=31.RHAP69FC10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTTAATNTGTTTTTATGATATATTGAATCTCGGTCTCATAATGAGACAGAACATGTGACATTTATTTTCTTCCCCAGTTATTCTATCTTGTTCCCAGTTCTCTTCCTTTGAACAATCAGTCTCCCTTTACAATCTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGAATACACCNAGAAATTTCAGTCCGGTGNGCGGTTGATTTCTGGGTATGTCCATATATGTANGTATGTGTATGTGAGTGTCTAGAGAGTTCACAAGAATGCATAGGCTCTTGGGGCTGAAGCTACAGGAAGTTCTCACTTCTCCAATTTGAGTGCTAAGGCATGGA
SEQUENCE_INCLUDED_REGION=18,354
SEQUENCE_TARGET=160,42
P3_COMMENT=CL is TCCCAGTTCTCTTCCTTTGAA
P3_COMMENT=CR is CATACCCAGAAATCAACCGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.435943
PRIMER_LEFT_0_PENALTY=2.105078
PRIMER_RIGHT_0_PENALTY=0.330865
PRIMER_LEFT_0_SEQUENCE=TCCCAGTTCTCTTCCTTTGAA
PRIMER_RIGHT_0_SEQUENCE=CATACCCAGAAATCAACCGC
PRIMER_LEFT_0=110,21
PRIMER_RIGHT_0=254,20
PRIMER_LEFT_0_TM=58.895
PRIMER_RIGHT_0_TM=60.331
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=11.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=145
PRIMER_PAIR_0_PRODUCT_TM=67.9
PRIMER_PAIR_1_PENALTY=2.435943
PRIMER_LEFT_1_PENALTY=2.105078
PRIMER_RIGHT_1_PENALTY=0.330865
PRIMER_LEFT_1_SEQUENCE=TTCCCAGTTCTCTTCCTTTGA
PRIMER_RIGHT_1_SEQUENCE=CATACCCAGAAATCAACCGC
PRIMER_LEFT_1=109,21
PRIMER_RIGHT_1=254,20
PRIMER_LEFT_1_TM=58.895
PRIMER_RIGHT_1_TM=60.331
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=11.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=146
PRIMER_PAIR_1_PRODUCT_TM=67.9
PRIMER_PAIR_2_PENALTY=2.544440
PRIMER_LEFT_2_PENALTY=2.213575
PRIMER_RIGHT_2_PENALTY=0.330865
PRIMER_LEFT_2_SEQUENCE=TTCCCAGTTCTCTTCCTTTGAA
PRIMER_RIGHT_2_SEQUENCE=CATACCCAGAAATCAACCGC
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=254,20
PRIMER_LEFT_2_TM=60.214
PRIMER_RIGHT_2_TM=60.331
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=146
PRIMER_PAIR_2_PRODUCT_TM=67.9
PRIMER_PAIR_3_PENALTY=2.601639
PRIMER_LEFT_3_PENALTY=2.270774
PRIMER_RIGHT_3_PENALTY=0.330865
PRIMER_LEFT_3_SEQUENCE=TCCCAGTTCTCTTCCTTTGAAC
PRIMER_RIGHT_3_SEQUENCE=CATACCCAGAAATCAACCGC
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=254,20
PRIMER_LEFT_3_TM=59.729
PRIMER_RIGHT_3_TM=60.331
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=11.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=145
PRIMER_PAIR_3_PRODUCT_TM=67.9
PRIMER_PAIR_4_PENALTY=2.601639
PRIMER_LEFT_4_PENALTY=2.270774
PRIMER_RIGHT_4_PENALTY=0.330865
PRIMER_LEFT_4_SEQUENCE=GTTCCCAGTTCTCTTCCTTTGA
PRIMER_RIGHT_4_SEQUENCE=CATACCCAGAAATCAACCGC
PRIMER_LEFT_4=108,22
PRIMER_RIGHT_4=254,20
PRIMER_LEFT_4_TM=59.729
PRIMER_RIGHT_4_TM=60.331
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=147
PRIMER_PAIR_4_PRODUCT_TM=68.1
=
SEQUENCE_ID=32.RHAP69FC11.seq
SEQUENCE_TEMPLATE=ANANTAAAGTCCCANCNNNTGTGGGNNGGAGGGTATCCTGGTTACATCAAACCTCTGCTGACTGAGGGCAATAGGGGCAGTATCAGAGAGGCCAAGAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGGGTGGAGGGTGCAGGATGATCCATGAGGGGAATCAGTTGTTGCTTCTGGAAAGATTGGGGTAACAGTCCAGAATATTCATATAGGCAGCAGCCCTGGTGGTTAGTGATGCTGGGATTTAAATCAGGGCTTCAACAGTGTTCGAAAATGTGAGGAAGGCGTCTGTTTCTCACCTCTGATCCTTATTATCAAGTCAGGAGAACTTT
SEQUENCE_INCLUDED_REGION=0,362
SEQUENCE_TARGET=101,52
P3_COMMENT=CL is AGGGCAATAGGGGCAGTATC
P3_COMMENT=CR is TGATTCCCCTCATGGATCAT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.402924
PRIMER_LEFT_0_PENALTY=0.306903
PRIMER_RIGHT_0_PENALTY=0.096021
PRIMER_LEFT_0_SEQUENCE=AGGGCAATAGGGGCAGTATC
PRIMER_RIGHT_0_SEQUENCE=TGATTCCCCTCATGGATCAT
PRIMER_LEFT_0=64,20
PRIMER_RIGHT_0=192,20
PRIMER_LEFT_0_TM=60.307
PRIMER_RIGHT_0_TM=60.096
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=5.3000
PRIMER_RIGHT_0_END_STABILITY=6.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=129
PRIMER_PAIR_0_PRODUCT_TM=69.6
PRIMER_PAIR_1_PENALTY=0.402924
PRIMER_LEFT_1_PENALTY=0.306903
PRIMER_RIGHT_1_PENALTY=0.096021
PRIMER_LEFT_1_SEQUENCE=GAGGGCAATAGGGGCAGTAT
PRIMER_RIGHT_1_SEQUENCE=TGATTCCCCTCATGGATCAT
PRIMER_LEFT_1=63,20
PRIMER_RIGHT_1=192,20
PRIMER_LEFT_1_TM=60.307
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=130
PRIMER_PAIR_1_PRODUCT_TM=69.8
PRIMER_PAIR_2_PENALTY=0.490847
PRIMER_LEFT_2_PENALTY=0.306903
PRIMER_RIGHT_2_PENALTY=0.183944
PRIMER_LEFT_2_SEQUENCE=AGGGCAATAGGGGCAGTATC
PRIMER_RIGHT_2_SEQUENCE=CCCAATCTTTCCAGAAGCAA
PRIMER_LEFT_2=64,20
PRIMER_RIGHT_2=216,20
PRIMER_LEFT_2_TM=60.307
PRIMER_RIGHT_2_TM=60.184
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=153
PRIMER_PAIR_2_PRODUCT_TM=71.0
PRIMER_PAIR_3_PENALTY=0.490847
PRIMER_LEFT_3_PENALTY=0.306903
PRIMER_RIGHT_3_PENALTY=0.183944
PRIMER_LEFT_3_SEQUENCE=GAGGGCAATAGGGGCAGTAT
PRIMER_RIGHT_3_SEQUENCE=CCCAATCTTTCCAGAAGCAA
PRIMER_LEFT_3=63,20
PRIMER_RIGHT_3=216,20
PRIMER_LEFT_3_TM=60.307
PRIMER_RIGHT_3_TM=60.184
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=154
PRIMER_PAIR_3_PRODUCT_TM=71.2
PRIMER_PAIR_4_PENALTY=0.527748
PRIMER_LEFT_4_PENALTY=0.306903
PRIMER_RIGHT_4_PENALTY=0.220845
PRIMER_LEFT_4_SEQUENCE=AGGGCAATAGGGGCAGTATC
PRIMER_RIGHT_4_SEQUENCE=ACAACTGATTCCCCTCATGG
PRIMER_LEFT_4=64,20
PRIMER_RIGHT_4=197,20
PRIMER_LEFT_4_TM=60.307
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=134
PRIMER_PAIR_4_PRODUCT_TM=69.8
=
SEQUENCE_ID=33.RHAP69FC12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNGCCTCCCAAGTCCCCTCAGCCTCCTCACCACCTTTTATCAGATGGAGTGGAGGAGATGGCAGTCTCTCCCTCCCAGAAGGCTTTGCAGGCTGCTGAAGCCCTTTCCTTCAGGCTGAGCAGCAGCAGCAGCAGCCCCCAACTGGGGCTGTATTATCATTCCATCTTGACCTCAAAGGAAAGAAAGTGAAGCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCACTCACATACTCACACGCGCGCGCNNNNNNNNNNNNNNNNCCCTGTCACCCCAGGCCTCTTATCTTGCTGAATCTGCACCATCTCTCATGAAAGAGGCATGCTT
SEQUENCE_INCLUDED_REGION=19,357
SEQUENCE_TARGET=212,54
SEQUENCE_TARGET=296,16
P3_COMMENT=CL is CTTTCCTTCAGGCTGAGCAG
P3_COMMENT=CR is GCGCGTGTGAGTATGTGAGT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.332354
PRIMER_LEFT_0_PENALTY=0.268570
PRIMER_RIGHT_0_PENALTY=0.063784
PRIMER_LEFT_0_SEQUENCE=CTTTCCTTCAGGCTGAGCAG
PRIMER_RIGHT_0_SEQUENCE=GCGCGTGTGAGTATGTGAGT
PRIMER_LEFT_0=119,20
PRIMER_RIGHT_0=291,20
PRIMER_LEFT_0_TM=60.269
PRIMER_RIGHT_0_TM=59.936
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=173
PRIMER_PAIR_0_PRODUCT_TM=71.1
PRIMER_PAIR_1_PENALTY=1.135890
PRIMER_LEFT_1_PENALTY=1.072106
PRIMER_RIGHT_1_PENALTY=0.063784
PRIMER_LEFT_1_SEQUENCE=CCCTTTCCTTCAGGCTGAG
PRIMER_RIGHT_1_SEQUENCE=GCGCGTGTGAGTATGTGAGT
PRIMER_LEFT_1=117,19
PRIMER_RIGHT_1=291,20
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=71.5
PRIMER_PAIR_2_PENALTY=1.144433
PRIMER_LEFT_2_PENALTY=1.080649
PRIMER_RIGHT_2_PENALTY=0.063784
PRIMER_LEFT_2_SEQUENCE=CCTTTCCTTCAGGCTGAGC
PRIMER_RIGHT_2_SEQUENCE=GCGCGTGTGAGTATGTGAGT
PRIMER_LEFT_2=118,19
PRIMER_RIGHT_2=291,20
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=174
PRIMER_PAIR_2_PRODUCT_TM=71.3
PRIMER_PAIR_3_PENALTY=1.223918
PRIMER_LEFT_3_PENALTY=1.160134
PRIMER_RIGHT_3_PENALTY=0.063784
PRIMER_LEFT_3_SEQUENCE=TGGGGCTGTATTATCATTCCA
PRIMER_RIGHT_3_SEQUENCE=GCGCGTGTGAGTATGTGAGT
PRIMER_LEFT_3=159,21
PRIMER_RIGHT_3=291,20
PRIMER_LEFT_3_TM=60.160
PRIMER_RIGHT_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=133
PRIMER_PAIR_3_PRODUCT_TM=66.8
PRIMER_PAIR_4_PENALTY=1.232805
PRIMER_LEFT_4_PENALTY=1.169021
PRIMER_RIGHT_4_PENALTY=0.063784
PRIMER_LEFT_4_SEQUENCE=CCAACTGGGGCTGTATTATCA
PRIMER_RIGHT_4_SEQUENCE=GCGCGTGTGAGTATGTGAGT
PRIMER_LEFT_4=154,21
PRIMER_RIGHT_4=291,20
PRIMER_LEFT_4_TM=59.831
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=67.4
=
SEQUENCE_ID=33.RHAP69FC6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTCTTTGTCCTGTGGCTCAGTTCAAACCATTCATCTCAGAGAAAAAAGCAGAATGGTATATGATAGGCTTGAAAAGTGGGACTTAGAGGATGAATAAGGAGAATAAAGGAATAGAAGACCATACTTAGGCGGAATAACTTCTAGTGTTCAATATGGTGTCTGACACTAATTAAGTGTATATTATATTGCCTATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAGCCACTTACAGAGAACTGAAGACATGAGGCAGAGTGAAAGGTCAAAACATTTGCAGATGAAGCAACCTTGACCCAGTGACTGCAGACTCACTTTTGTTCTATCCCATCCAGTTCCTCAAAACTCAGACGGCCTGACACTCACTAGCTAAAAATAACATGATCTTTTGCAAAGGAAATATTTAAGGCTTCCTATTA
SEQUENCE_INCLUDED_REGION=31,449
SEQUENCE_TARGET=231,50
P3_COMMENT=CL is GGCGGAATAACTTCTAGTGTTCA
P3_COMMENT=CR is TGACCTTTCACTCTGCCTCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.774898
PRIMER_LEFT_0_PENALTY=3.320295
PRIMER_RIGHT_0_PENALTY=0.454603
PRIMER_LEFT_0_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
PRIMER_RIGHT_0_SEQUENCE=TGACCTTTCACTCTGCCTCA
PRIMER_LEFT_0=161,23
PRIMER_RIGHT_0=328,20
PRIMER_LEFT_0_TM=59.680
PRIMER_RIGHT_0_TM=59.545
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=168
PRIMER_PAIR_0_PRODUCT_TM=67.1
PRIMER_PAIR_1_PENALTY=4.098231
PRIMER_LEFT_1_PENALTY=2.659428
PRIMER_RIGHT_1_PENALTY=1.438803
PRIMER_LEFT_1_SEQUENCE=GAAGACCATACTTAGGCGGAA
PRIMER_RIGHT_1_SEQUENCE=TCACTCTGCCTCATGTCTTCA
PRIMER_LEFT_1=147,21
PRIMER_RIGHT_1=321,21
PRIMER_LEFT_1_TM=58.341
PRIMER_RIGHT_1_TM=59.561
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=67.3
PRIMER_PAIR_2_PENALTY=4.461250
PRIMER_LEFT_2_PENALTY=3.320295
PRIMER_RIGHT_2_PENALTY=1.140954
PRIMER_LEFT_2_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
PRIMER_RIGHT_2_SEQUENCE=CCTTTCACTCTGCCTCATGTC
PRIMER_LEFT_2=161,23
PRIMER_RIGHT_2=325,21
PRIMER_LEFT_2_TM=59.680
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=165
PRIMER_PAIR_2_PRODUCT_TM=67.0
PRIMER_PAIR_3_PENALTY=4.461250
PRIMER_LEFT_3_PENALTY=3.320295
PRIMER_RIGHT_3_PENALTY=1.140954
PRIMER_LEFT_3_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
PRIMER_RIGHT_3_SEQUENCE=GACCTTTCACTCTGCCTCATG
PRIMER_LEFT_3=161,23
PRIMER_RIGHT_3=327,21
PRIMER_LEFT_3_TM=59.680
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=167
PRIMER_PAIR_3_PRODUCT_TM=67.1
PRIMER_PAIR_4_PENALTY=4.461250
PRIMER_LEFT_4_PENALTY=3.320295
PRIMER_RIGHT_4_PENALTY=1.140954
PRIMER_LEFT_4_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
PRIMER_RIGHT_4_SEQUENCE=TGACCTTTCACTCTGCCTCAT
PRIMER_LEFT_4=161,23
PRIMER_RIGHT_4=328,21
PRIMER_LEFT_4_TM=59.680
PRIMER_RIGHT_4_TM=59.859
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=168
PRIMER_PAIR_4_PRODUCT_TM=67.1
=
SEQUENCE_ID=35.RHAP69FD11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTTGCTCTTACATGTCTGACCTGCTTGTCCTGGTGGTTAGAACCACTTCACATTGTTATCTTACAGTTTTATTGATGGGTTGCCCATTTGCTCATTCTTTGGGACAGAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCCCTCTTCCTCACTGGCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=19,173
SEQUENCE_TARGET=133,34
P3_COMMENT=CL is TGTCTGACCTGCTTGTCCTG
P3_COMMENT=CR is ACCCAGCCAGTGAGGAAGAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.792553
PRIMER_LEFT_0_PENALTY=0.022009
PRIMER_RIGHT_0_PENALTY=1.770544
PRIMER_LEFT_0_SEQUENCE=TGTCTGACCTGCTTGTCCTG
PRIMER_RIGHT_0_SEQUENCE=ACCCAGCCAGTGAGGAAGAG
PRIMER_LEFT_0=30,20
PRIMER_RIGHT_0=191,20
PRIMER_LEFT_0_TM=60.022
PRIMER_RIGHT_0_TM=61.771
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=162
PRIMER_PAIR_0_PRODUCT_TM=71.4
PRIMER_PAIR_1_PENALTY=1.834007
PRIMER_LEFT_1_PENALTY=0.022009
PRIMER_RIGHT_1_PENALTY=1.811998
PRIMER_LEFT_1_SEQUENCE=TGTCTGACCTGCTTGTCCTG
PRIMER_RIGHT_1_SEQUENCE=ACCCAGCCAGTGAGGAAGA
PRIMER_LEFT_1=30,20
PRIMER_RIGHT_1=191,19
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=60.812
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=162
PRIMER_PAIR_1_PRODUCT_TM=71.4
PRIMER_PAIR_2_PENALTY=1.971300
PRIMER_LEFT_2_PENALTY=0.022009
PRIMER_RIGHT_2_PENALTY=1.949291
PRIMER_LEFT_2_SEQUENCE=TGTCTGACCTGCTTGTCCTG
PRIMER_RIGHT_2_SEQUENCE=CAGCCAGTGAGGAAGAGGG
PRIMER_LEFT_2=30,20
PRIMER_RIGHT_2=188,19
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=60.949
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=63.158
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=159
PRIMER_PAIR_2_PRODUCT_TM=71.1
PRIMER_PAIR_3_PENALTY=1.971300
PRIMER_LEFT_3_PENALTY=0.022009
PRIMER_RIGHT_3_PENALTY=1.949291
PRIMER_LEFT_3_SEQUENCE=TGTCTGACCTGCTTGTCCTG
PRIMER_RIGHT_3_SEQUENCE=CCAGCCAGTGAGGAAGAGG
PRIMER_LEFT_3=30,20
PRIMER_RIGHT_3=189,19
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=60.949
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=160
PRIMER_PAIR_3_PRODUCT_TM=71.3
PRIMER_PAIR_4_PENALTY=1.971300
PRIMER_LEFT_4_PENALTY=0.022009
PRIMER_RIGHT_4_PENALTY=1.949291
PRIMER_LEFT_4_SEQUENCE=TGTCTGACCTGCTTGTCCTG
PRIMER_RIGHT_4_SEQUENCE=CCCAGCCAGTGAGGAAGAG
PRIMER_LEFT_4=30,20
PRIMER_RIGHT_4=190,19
PRIMER_LEFT_4_TM=60.022
PRIMER_RIGHT_4_TM=60.949
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=161
PRIMER_PAIR_4_PRODUCT_TM=71.5
=
SEQUENCE_ID=39.RHAP69FE6.seq
SEQUENCE_TEMPLATE=CNCTNNNNNNNNNNNNNNNNNCTCTGNACACATTCGGACACANATTNTTNACACACNTTTTCTGTGACACACATACNCAGNNAGATACACACNTAACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACNGACTGTCAANNNNNNNNNNNNNNNNNNNNNNCTATACNGACGNNNNNNNNNNNNNNCTACAAACGGGACATAAAGCGGGACACGATGANAGGCTTTTCCNCNCACACNAAGTGATANCNNACAGCNTCNCACAGACANGNNNNCGNAGTGNTTGANNCNGACGCGCNCTCNTNANNGGAGGCACGGTCTAGNNNGCGCAGGTCTNGAAGGTCGGNTTAGNGNTGNNNNTANNGNNGCNCTTACNAACGCNGANTCATANTNTNTCAGANNNNGAGGGTNTNTTATNTAAAGNNTCAANANAAANAA
SEQUENCE_INCLUDED_REGION=0,442
SEQUENCE_TARGET=5,16
SEQUENCE_TARGET=99,32
SEQUENCE_TARGET=145,22
SEQUENCE_TARGET=178,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=40.RHAP69FF10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTNNTAAAAAATTGTCTTCAATATCTTTTTAAACCAATTAAATTTTTTAATTAAAATATAATTACATCACATCCTTTTTCCCTTTTGTCCCTCCAATCCCTCCCATCCTTTTCCATCACTTTCTTCCAAATTGATGGCCTCGTTTTCTTTGATGATTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACGCATGCACGCGCGCGCGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAACCTTCTATATCCATTTAGTGCTGCTTGTATATGTTTTCACAGTAGAGCACTTTGTATTGGATAACCAATTAGGTTGCTCATCCCTG
SEQUENCE_INCLUDED_REGION=19,361
SEQUENCE_TARGET=183,40
SEQUENCE_TARGET=248,40
P3_COMMENT=CL is CAAATTGATGGCCTCGTTTT
P3_COMMENT=CR is GCAGCACTAAATGGATATAGAAGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.610585
PRIMER_LEFT_0_PENALTY=0.061961
PRIMER_RIGHT_0_PENALTY=5.548624
PRIMER_LEFT_0_SEQUENCE=CAAATTGATGGCCTCGTTTT
PRIMER_RIGHT_0_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
PRIMER_LEFT_0=146,20
PRIMER_RIGHT_0=317,24
PRIMER_LEFT_0_TM=59.938
PRIMER_RIGHT_0_TM=58.451
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=172
PRIMER_PAIR_0_PRODUCT_TM=66.7
PRIMER_PAIR_1_PENALTY=5.740304
PRIMER_LEFT_1_PENALTY=0.191680
PRIMER_RIGHT_1_PENALTY=5.548624
PRIMER_LEFT_1_SEQUENCE=GGCCTCGTTTTCTTTGATGA
PRIMER_RIGHT_1_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
PRIMER_LEFT_1=155,20
PRIMER_RIGHT_1=317,24
PRIMER_LEFT_1_TM=60.192
PRIMER_RIGHT_1_TM=58.451
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=163
PRIMER_PAIR_1_PRODUCT_TM=66.5
PRIMER_PAIR_2_PENALTY=5.740304
PRIMER_LEFT_2_PENALTY=0.191680
PRIMER_RIGHT_2_PENALTY=5.548624
PRIMER_LEFT_2_SEQUENCE=GATGGCCTCGTTTTCTTTGA
PRIMER_RIGHT_2_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
PRIMER_LEFT_2=152,20
PRIMER_RIGHT_2=317,24
PRIMER_LEFT_2_TM=60.192
PRIMER_RIGHT_2_TM=58.451
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=166
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=5.762908
PRIMER_LEFT_3_PENALTY=0.061961
PRIMER_RIGHT_3_PENALTY=5.700947
PRIMER_LEFT_3_SEQUENCE=CAAATTGATGGCCTCGTTTT
PRIMER_RIGHT_3_SEQUENCE=AGCAGCACTAAATGGATATAGAAGG
PRIMER_LEFT_3=146,20
PRIMER_RIGHT_3=318,25
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=59.299
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=173
PRIMER_PAIR_3_PRODUCT_TM=66.6
PRIMER_PAIR_4_PENALTY=5.766630
PRIMER_LEFT_4_PENALTY=0.191680
PRIMER_RIGHT_4_PENALTY=5.574950
PRIMER_LEFT_4_SEQUENCE=GGCCTCGTTTTCTTTGATGA
PRIMER_RIGHT_4_SEQUENCE=CATATACAAGCAGCACTAAATGGA
PRIMER_LEFT_4=155,20
PRIMER_RIGHT_4=326,24
PRIMER_LEFT_4_TM=60.192
PRIMER_RIGHT_4_TM=58.425
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=66.7
=
SEQUENCE_ID=40.RHAP69FF4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCACGCACACATCTGCATACTNNNNNNNNNNNNNNNNNNNNNNNNGACATACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAATGGGCCCTTTGGCTTTTTTCTGATGACCTATCACCATCTCAAGTTCAAGACCCTGCACAGACCAGGTAATTTAAGAATCATCACCTTAAATTCAAGTTGCACCTTATGATTTAATTATTTCATGGAGATGGCTTTCTCCTTTCCAGTTTTATTCTCCATAAACTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=17,308
SEQUENCE_TARGET=17,21
SEQUENCE_TARGET=60,24
SEQUENCE_TARGET=93,60
P3_COMMENT=CL is GCACGCACACATCTGCATAC
P3_COMMENT=CR is TGCAGGGTCTTGAACTTGAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.337718
PRIMER_LEFT_0_PENALTY=1.344726
PRIMER_RIGHT_0_PENALTY=0.992992
PRIMER_LEFT_0_SEQUENCE=GCACGCACACATCTGCATAC
PRIMER_RIGHT_0_SEQUENCE=TGCAGGGTCTTGAACTTGAG
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=213,20
PRIMER_LEFT_0_TM=61.345
PRIMER_RIGHT_0_TM=59.007
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=175
PRIMER_PAIR_0_PRODUCT_TM=66.8
PRIMER_PAIR_1_PENALTY=2.451371
PRIMER_LEFT_1_PENALTY=1.458379
PRIMER_RIGHT_1_PENALTY=0.992992
PRIMER_LEFT_1_SEQUENCE=GCACGCACACATCTGCATA
PRIMER_RIGHT_1_SEQUENCE=TGCAGGGTCTTGAACTTGAG
PRIMER_LEFT_1=39,19
PRIMER_RIGHT_1=213,20
PRIMER_LEFT_1_TM=60.458
PRIMER_RIGHT_1_TM=59.007
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=66.8
PRIMER_PAIR_2_PENALTY=2.535897
PRIMER_LEFT_2_PENALTY=1.344726
PRIMER_RIGHT_2_PENALTY=1.191170
PRIMER_LEFT_2_SEQUENCE=GCACGCACACATCTGCATAC
PRIMER_RIGHT_2_SEQUENCE=CAGAAAAAAGCCAAAGGGC
PRIMER_LEFT_2=39,20
PRIMER_RIGHT_2=178,19
PRIMER_LEFT_2_TM=61.345
PRIMER_RIGHT_2_TM=59.809
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=47.368
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=140
PRIMER_PAIR_2_PRODUCT_TM=63.8
PRIMER_PAIR_3_PENALTY=2.649549
PRIMER_LEFT_3_PENALTY=1.458379
PRIMER_RIGHT_3_PENALTY=1.191170
PRIMER_LEFT_3_SEQUENCE=GCACGCACACATCTGCATA
PRIMER_RIGHT_3_SEQUENCE=CAGAAAAAAGCCAAAGGGC
PRIMER_LEFT_3=39,19
PRIMER_RIGHT_3=178,19
PRIMER_LEFT_3_TM=60.458
PRIMER_RIGHT_3_TM=59.809
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=47.368
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=140
PRIMER_PAIR_3_PRODUCT_TM=63.8
PRIMER_PAIR_4_PENALTY=2.687253
PRIMER_LEFT_4_PENALTY=1.694261
PRIMER_RIGHT_4_PENALTY=0.992992
PRIMER_LEFT_4_SEQUENCE=CACGCACACATCTGCATACT
PRIMER_RIGHT_4_SEQUENCE=TGCAGGGTCTTGAACTTGAG
PRIMER_LEFT_4=40,20
PRIMER_RIGHT_4=213,20
PRIMER_LEFT_4_TM=58.306
PRIMER_RIGHT_4_TM=59.007
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=66.6
=
SEQUENCE_ID=41.RHAP69FF11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTAAGAGCTTAACAACTTATGGCAGCTTTTTAAAATCTGTCCACTTTTCTAAGTGCACTGTCTTCTTTGTCTCCCTTCTATCGTAGTTTTAAGCACATTTTCCTTCACACTTTGAGTTTTCNNNNNNNNNNNNNNNCATGACTCCTCTGCCATTATGTGACTGTTGTCCACTATGTGGCTGACCTCAATGTGGGGTTAACTCACATGACATGATTTTTTCCCCCTTTTCTCCCCTTCATCTTCCTTCTCTAGGCACCTGCTGAGATTTACAGCCTTGGGGATATTTTTTAAGCCTGAAAAGNNNNNNNNNNNNNNNNNNNNACAGNNNNNNNNNNNNNNNNNGNCANNNCGCTACNGNGNG
SEQUENCE_INCLUDED_REGION=18,360
SEQUENCE_TARGET=138,15
SEQUENCE_TARGET=318,20
SEQUENCE_TARGET=342,17
P3_COMMENT=CL is GCACTGTCTTCTTTGTCTCCC
P3_COMMENT=CR is TGAGGTCAGCCACATAGTGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.367400
PRIMER_LEFT_0_PENALTY=2.068459
PRIMER_RIGHT_0_PENALTY=0.298942
PRIMER_LEFT_0_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
PRIMER_RIGHT_0_SEQUENCE=TGAGGTCAGCCACATAGTGG
PRIMER_LEFT_0=71,21
PRIMER_RIGHT_0=203,20
PRIMER_LEFT_0_TM=58.932
PRIMER_RIGHT_0_TM=59.701
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=133
PRIMER_PAIR_0_PRODUCT_TM=71.1
PRIMER_PAIR_1_PENALTY=2.376282
PRIMER_LEFT_1_PENALTY=2.077340
PRIMER_RIGHT_1_PENALTY=0.298942
PRIMER_LEFT_1_SEQUENCE=GCACTGTCTTCTTTGTCTCCCT
PRIMER_RIGHT_1_SEQUENCE=TGAGGTCAGCCACATAGTGG
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=203,20
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=59.701
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=133
PRIMER_PAIR_1_PRODUCT_TM=71.1
PRIMER_PAIR_2_PENALTY=2.494227
PRIMER_LEFT_2_PENALTY=2.345718
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=GCACATTTTCCTTCACACTTTG
PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCCTAGAGAAGG
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=278,20
PRIMER_LEFT_2_TM=59.654
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=170
PRIMER_PAIR_2_PRODUCT_TM=73.5
PRIMER_PAIR_3_PENALTY=2.517268
PRIMER_LEFT_3_PENALTY=2.068459
PRIMER_RIGHT_3_PENALTY=0.448810
PRIMER_LEFT_3_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
PRIMER_RIGHT_3_SEQUENCE=GGTCAGCCACATAGTGGACA
PRIMER_LEFT_3=71,21
PRIMER_RIGHT_3=200,20
PRIMER_LEFT_3_TM=58.932
PRIMER_RIGHT_3_TM=59.551
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=130
PRIMER_PAIR_3_PRODUCT_TM=71.1
PRIMER_PAIR_4_PENALTY=2.517268
PRIMER_LEFT_4_PENALTY=2.068459
PRIMER_RIGHT_4_PENALTY=0.448810
PRIMER_LEFT_4_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
PRIMER_RIGHT_4_SEQUENCE=CACATTGAGGTCAGCCACAT
PRIMER_LEFT_4=71,21
PRIMER_RIGHT_4=208,20
PRIMER_LEFT_4_TM=58.932
PRIMER_RIGHT_4_TM=59.551
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=71.3
=
SEQUENCE_ID=42.RHAP69FF6.seq
SEQUENCE_TEMPLATE=CACTNNNNNNNNNNNNNNNNCACTGNCACACGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAACCAGACAGAGAGTTGACACATTCACAAGTTCAAGACGCAAAGTGTTCTTTTCTTCGAATTACACTTCCTTGAGTTTCGCCTGGCCTTGGGTTGTTTTGAAGTTGGCAAGTCTCCTCAAAGCTGCTTTTGAGAGCCACCGTGTTCATTAGCTGGTTCACAGCTTCCTCCTACCACTTAGCTTGCCTGGAACCCTGCTGTGCAAACAGGCCTGGTGAGCTGGGATGTCTGGCACGTGCAAGGCCCAGGTTCCACCCTGGCACAGTGAGGAGGAAGCAGGCCTTGTGTGTGGACACCCAGTCCCTGTGGCACTTAGAGCCTACCNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,428
SEQUENCE_TARGET=4,16
SEQUENCE_TARGET=35,40
SEQUENCE_TARGET=402,26
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=44.RHAP69FG5.seq
SEQUENCE_TEMPLATE=AANTNTATNNTTGNNAGGNNNANGNANGGAGNNNNNNNGGANNGNNNNNTANNGTNNAANNNGNTNGGGNNNTNGGNNNNANNNACNNNNTCTNAANNTCTATTNANTNCCACNTNNTGGGGNNAACCATNCANTNNCAGNTGTGCTTNNGAGGGTGGNNTTCTGATTTNAAAACCAANCAACANNAANNNATTAGANANNNNNNNTNNCACAACACNCATNANNCNNNNNNNNNNNNNNNNNNNACNCNNAAACTCCANTCACNCANAAATTATTCTTCTTCNCCAGCNANTTCATGAAANTTTTCTTCAAGAACCACATTACTTTGGGCACAAAAATAAGGCTCNTCAGGATGACAAAGAAAAATTGNACTAGGNACTGGNTTAAAAGAANAGCATAATTCAAACAATATAATGNTGGGNAAAANTGAATGGGCTGACAGATAGAAGAACTTGATCANCANGGAACANNATTTNAACTTCAAGTTAATNAAAAACNC
SEQUENCE_INCLUDED_REGION=0,499
SEQUENCE_TARGET=228,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=01.RHAP131FA1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCNTTAAGGATTACAANACTGAACCACCAACCAANNAGCATGCATNGGNNTGACAANGNCNNNNNNNNNNNNNNNACACNCTCGNCNCNCTCNCACNCACNCNCTCTCNNNCNTCTNTATNTAGNGNGGTATANTTGNGTCNCTAAANNTAGAATGTTNNNTNNGNNATNTTTNNANANGGGGGNNGNTNACCTACANGNNCNCTNTTNGCTAACNNTTTTGTTATTGCCANTCAACTNNAANNTTTGGGNGNCNNNNTTNTTGNTCTAGANTTTTNAATGTNNANAAAAANANNNAANNTNNTAAGANTNNNNTNNGGTNNNNTNNTAAAAGGGNGTTTTTCNNCNAATAANANNGGGGNAANANNNNAGGGGGAGNNNNTNANAAAANNNGGGGNTNNTTGNANNNNNNAATANNNATAGNGTTNAANNNNAATNNNNTTCAATTNNNAAATGNNAAGNGTTNNAAAAAAANTGTTNNTNNNTTNNTTNAAANTGATGANTNTGNNTANNACNAANTTTCTNNGNNGAGAANNCCGAAGCCTCCNAAANNCTNTNAATTNGNNNAANCAANAANNNGNNGGCNNAAANAAANNATTGTTNNNCCCNNNNAAAATTNCTCNNCCNNNAANNANGACGNNNNNANNATNNNNGGNNANATNCNNTGGGGNAGCNTNAATTNNATGANNTTNNNNNACAAANNAATTNGANNANNCTA
SEQUENCE_INCLUDED_REGION=23,717
SEQUENCE_TARGET=84,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=01.RHAP131FA7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCAGTATAAGTATGTCTGGAGATCTTTTAGAAATGAGGGGTAAATTCTGAGGGCAGGAAGGTTTTAGGGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTGCCTGCAAATTGGTATAGGCTGAGAAATTCAATTAATCGTATTCATCAATTTTTCACTCTGGGGGGGGAAATGAGGCAGAGATGTGGAATTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=23,224
SEQUENCE_TARGET=95,50
P3_COMMENT=CL is GAGGGCAGGAAGGTTTTAGG
P3_COMMENT=CR is CCCCCAGAGTGAAAAATTGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.164371
PRIMER_LEFT_0_PENALTY=0.067516
PRIMER_RIGHT_0_PENALTY=0.096855
PRIMER_LEFT_0_SEQUENCE=GAGGGCAGGAAGGTTTTAGG
PRIMER_RIGHT_0_SEQUENCE=CCCCCAGAGTGAAAAATTGA
PRIMER_LEFT_0=70,20
PRIMER_RIGHT_0=215,20
PRIMER_LEFT_0_TM=60.068
PRIMER_RIGHT_0_TM=59.903
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.5000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=146
PRIMER_PAIR_0_PRODUCT_TM=67.0
PRIMER_PAIR_1_PENALTY=0.170721
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.096855
PRIMER_LEFT_1_SEQUENCE=ATTCTGAGGGCAGGAAGGTT
PRIMER_RIGHT_1_SEQUENCE=CCCCCAGAGTGAAAAATTGA
PRIMER_LEFT_1=65,20
PRIMER_RIGHT_1=215,20
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.903
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=151
PRIMER_PAIR_1_PRODUCT_TM=67.1
PRIMER_PAIR_2_PENALTY=0.170721
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.096855
PRIMER_LEFT_2_SEQUENCE=AATTCTGAGGGCAGGAAGGT
PRIMER_RIGHT_2_SEQUENCE=CCCCCAGAGTGAAAAATTGA
PRIMER_LEFT_2=64,20
PRIMER_RIGHT_2=215,20
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.903
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=152
PRIMER_PAIR_2_PRODUCT_TM=67.0
PRIMER_PAIR_3_PENALTY=0.193675
PRIMER_LEFT_3_PENALTY=0.096820
PRIMER_RIGHT_3_PENALTY=0.096855
PRIMER_LEFT_3_SEQUENCE=GAGGGGTAAATTCTGAGGGC
PRIMER_RIGHT_3_SEQUENCE=CCCCCAGAGTGAAAAATTGA
PRIMER_LEFT_3=56,20
PRIMER_RIGHT_3=215,20
PRIMER_LEFT_3_TM=59.903
PRIMER_RIGHT_3_TM=59.903
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=160
PRIMER_PAIR_3_PRODUCT_TM=67.9
PRIMER_PAIR_4_PENALTY=0.519743
PRIMER_LEFT_4_PENALTY=0.422888
PRIMER_RIGHT_4_PENALTY=0.096855
PRIMER_LEFT_4_SEQUENCE=GCAGGAAGGTTTTAGGGGAA
PRIMER_RIGHT_4_SEQUENCE=CCCCCAGAGTGAAAAATTGA
PRIMER_LEFT_4=74,20
PRIMER_RIGHT_4=215,20
PRIMER_LEFT_4_TM=60.423
PRIMER_RIGHT_4_TM=59.903
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=142
PRIMER_PAIR_4_PRODUCT_TM=66.4
=
SEQUENCE_ID=02.RHAP131FA8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAGTATAAGTATGTCTGGANTAATAATAGCAAAAAGTATAAAACCATGAGGGCAGGAAGGGTGGCTCCACTGGGATTACCTGCTGGAACCTCCTCCCTTTCCACAGGAACCTGAGCTTGTGACAAACTAATAAAATATGGAATTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCAGAAGAACAGGGAGATCTTGGAGGACCCATTCCAAGCATTGTTTATTCATTCATGTGGACACCGTGACTTGGGGGCTGGAGAGAGGGTGCAGCAGTTGAGAATAGACAACTCTTCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=22,336
SEQUENCE_TARGET=170,61
P3_COMMENT=CL is TTCCACAGGAACCTGAGCTT
P3_COMMENT=CR is AACAATGCTTGGAATGGGTC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.359427
PRIMER_LEFT_0_PENALTY=0.156061
PRIMER_RIGHT_0_PENALTY=0.203367
PRIMER_LEFT_0_SEQUENCE=TTCCACAGGAACCTGAGCTT
PRIMER_RIGHT_0_SEQUENCE=AACAATGCTTGGAATGGGTC
PRIMER_LEFT_0=121,20
PRIMER_RIGHT_0=279,20
PRIMER_LEFT_0_TM=59.844
PRIMER_RIGHT_0_TM=59.797
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=159
PRIMER_PAIR_0_PRODUCT_TM=66.7
PRIMER_PAIR_1_PENALTY=0.359427
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.203367
PRIMER_LEFT_1_SEQUENCE=TTTCCACAGGAACCTGAGCT
PRIMER_RIGHT_1_SEQUENCE=AACAATGCTTGGAATGGGTC
PRIMER_LEFT_1=120,20
PRIMER_RIGHT_1=279,20
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=59.797
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=160
PRIMER_PAIR_1_PRODUCT_TM=66.7
PRIMER_PAIR_2_PENALTY=0.359427
PRIMER_LEFT_2_PENALTY=0.156061
PRIMER_RIGHT_2_PENALTY=0.203367
PRIMER_LEFT_2_SEQUENCE=CTTTCCACAGGAACCTGAGC
PRIMER_RIGHT_2_SEQUENCE=AACAATGCTTGGAATGGGTC
PRIMER_LEFT_2=119,20
PRIMER_RIGHT_2=279,20
PRIMER_LEFT_2_TM=59.844
PRIMER_RIGHT_2_TM=59.797
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=161
PRIMER_PAIR_2_PRODUCT_TM=66.9
PRIMER_PAIR_3_PENALTY=0.434225
PRIMER_LEFT_3_PENALTY=0.156061
PRIMER_RIGHT_3_PENALTY=0.278164
PRIMER_LEFT_3_SEQUENCE=TTCCACAGGAACCTGAGCTT
PRIMER_RIGHT_3_SEQUENCE=ATGGGTCCTCCAAGATCTCC
PRIMER_LEFT_3=121,20
PRIMER_RIGHT_3=266,20
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=60.278
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=146
PRIMER_PAIR_3_PRODUCT_TM=65.9
PRIMER_PAIR_4_PENALTY=0.434225
PRIMER_LEFT_4_PENALTY=0.156061
PRIMER_RIGHT_4_PENALTY=0.278164
PRIMER_LEFT_4_SEQUENCE=TTTCCACAGGAACCTGAGCT
PRIMER_RIGHT_4_SEQUENCE=ATGGGTCCTCCAAGATCTCC
PRIMER_LEFT_4=120,20
PRIMER_RIGHT_4=266,20
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=60.278
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=147
PRIMER_PAIR_4_PRODUCT_TM=65.9
=
SEQUENCE_ID=04.RHAP131FB7.seq
SEQUENCE_TEMPLATE=TCTAGGACCCCCCTATTTGGGCTCTTCTCNCCGGGTCCTCTGAAAGAACAGCCAGTCTTCCTTTACACTGGAAAGCAGAGAGTGAGTCATTTAGAGGGTCTTATGACCTGCTTCCTGGGAAAGTCAAGATGTTCTTCCTATTTTTAATGATCTACTTTAAATAGGGAAAAATACAAGGACAGAGAAGATTATTTTTGCTTGTGACATTTCCTTCACCAAATTCCTGCAGCTTAAAATACTAGTGTGCCAAAGTATGGTAAAATGTTCTTGAGTTCATCAATACAACTATTTTCTTAAAAGAATTCTTTATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGTGAGCGAGCAGTCTCTCTGTGCATGGAAGAATTGGGGGATTGAACCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,422
SEQUENCE_TARGET=313,50
P3_COMMENT=CL is CCAAATTCCTGCAGCTTAAAA
P3_COMMENT=CR is AGAGAGACTGCTCGCTCACC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.738003
PRIMER_LEFT_0_PENALTY=1.628243
PRIMER_RIGHT_0_PENALTY=0.109760
PRIMER_LEFT_0_SEQUENCE=CCAAATTCCTGCAGCTTAAAA
PRIMER_RIGHT_0_SEQUENCE=AGAGAGACTGCTCGCTCACC
PRIMER_LEFT_0=215,21
PRIMER_RIGHT_0=388,20
PRIMER_LEFT_0_TM=59.372
PRIMER_RIGHT_0_TM=59.890
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_0_PRODUCT_TM=67.3
PRIMER_PAIR_1_PENALTY=2.065161
PRIMER_LEFT_1_PENALTY=1.955401
PRIMER_RIGHT_1_PENALTY=0.109760
PRIMER_LEFT_1_SEQUENCE=CCAAATTCCTGCAGCTTAAA
PRIMER_RIGHT_1_SEQUENCE=AGAGAGACTGCTCGCTCACC
PRIMER_LEFT_1=215,20
PRIMER_RIGHT_1=388,20
PRIMER_LEFT_1_TM=58.045
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=174
PRIMER_PAIR_1_PRODUCT_TM=67.3
PRIMER_PAIR_2_PENALTY=2.221575
PRIMER_LEFT_2_PENALTY=2.111815
PRIMER_RIGHT_2_PENALTY=0.109760
PRIMER_LEFT_2_SEQUENCE=ACCAAATTCCTGCAGCTTAAA
PRIMER_RIGHT_2_SEQUENCE=AGAGAGACTGCTCGCTCACC
PRIMER_LEFT_2=214,21
PRIMER_RIGHT_2=388,20
PRIMER_LEFT_2_TM=58.888
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=175
PRIMER_PAIR_2_PRODUCT_TM=67.3
PRIMER_PAIR_3_PENALTY=2.237688
PRIMER_LEFT_3_PENALTY=2.127927
PRIMER_RIGHT_3_PENALTY=0.109760
PRIMER_LEFT_3_SEQUENCE=ACCAAATTCCTGCAGCTTAAAA
PRIMER_RIGHT_3_SEQUENCE=AGAGAGACTGCTCGCTCACC
PRIMER_LEFT_3=214,22
PRIMER_RIGHT_3=388,20
PRIMER_LEFT_3_TM=60.128
PRIMER_RIGHT_3_TM=59.890
PRIMER_LEFT_3_GC_PERCENT=36.364
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=175
PRIMER_PAIR_3_PRODUCT_TM=67.3
PRIMER_PAIR_4_PENALTY=2.465353
PRIMER_LEFT_4_PENALTY=2.355593
PRIMER_RIGHT_4_PENALTY=0.109760
PRIMER_LEFT_4_SEQUENCE=CCAAATTCCTGCAGCTTAAAAT
PRIMER_RIGHT_4_SEQUENCE=AGAGAGACTGCTCGCTCACC
PRIMER_LEFT_4=215,22
PRIMER_RIGHT_4=388,20
PRIMER_LEFT_4_TM=59.644
PRIMER_RIGHT_4_TM=59.890
PRIMER_LEFT_4_GC_PERCENT=36.364
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=67.3
=
SEQUENCE_ID=08.RHAP131FC2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAGGATATTTTGCCCTTGCAAGGAACATACTACACAGCTCATGCCAAGTTTTTCATGGTTTTTGTGAGAAAATTTTAGGTGCAGCAAAAGAGACGATTGGTTACATTCTCACAGCTGATTAGCTCCAACACAGAGTGGTATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGGTGGGAAGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=22,210
SEQUENCE_TARGET=167,46
P3_COMMENT=CL is TGCAGCAAAAGAGACGATTG
P3_COMMENT=CR is CCTTCTCTTCCCACCCATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.689198
PRIMER_LEFT_0_PENALTY=0.134363
PRIMER_RIGHT_0_PENALTY=1.554836
PRIMER_LEFT_0_SEQUENCE=TGCAGCAAAAGAGACGATTG
PRIMER_RIGHT_0_SEQUENCE=CCTTCTCTTCCCACCCATC
PRIMER_LEFT_0=102,20
PRIMER_RIGHT_0=231,19
PRIMER_LEFT_0_TM=60.134
PRIMER_RIGHT_0_TM=59.445
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=130
PRIMER_PAIR_0_PRODUCT_TM=67.3
PRIMER_PAIR_1_PENALTY=1.960552
PRIMER_LEFT_1_PENALTY=0.405717
PRIMER_RIGHT_1_PENALTY=1.554836
PRIMER_LEFT_1_SEQUENCE=GGTGCAGCAAAAGAGACGAT
PRIMER_RIGHT_1_SEQUENCE=CCTTCTCTTCCCACCCATC
PRIMER_LEFT_1=100,20
PRIMER_RIGHT_1=231,19
PRIMER_LEFT_1_TM=60.406
PRIMER_RIGHT_1_TM=59.445
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=132
PRIMER_PAIR_1_PRODUCT_TM=67.8
PRIMER_PAIR_2_PENALTY=2.036792
PRIMER_LEFT_2_PENALTY=0.481956
PRIMER_RIGHT_2_PENALTY=1.554836
PRIMER_LEFT_2_SEQUENCE=TCATGCCAAGTTTTTCATGG
PRIMER_RIGHT_2_SEQUENCE=CCTTCTCTTCCCACCCATC
PRIMER_LEFT_2=61,20
PRIMER_RIGHT_2=231,19
PRIMER_LEFT_2_TM=59.518
PRIMER_RIGHT_2_TM=59.445
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=68.6
PRIMER_PAIR_3_PENALTY=2.334969
PRIMER_LEFT_3_PENALTY=0.780133
PRIMER_RIGHT_3_PENALTY=1.554836
PRIMER_LEFT_3_SEQUENCE=TAGGTGCAGCAAAAGAGACG
PRIMER_RIGHT_3_SEQUENCE=CCTTCTCTTCCCACCCATC
PRIMER_LEFT_3=98,20
PRIMER_RIGHT_3=231,19
PRIMER_LEFT_3_TM=59.220
PRIMER_RIGHT_3_TM=59.445
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=134
PRIMER_PAIR_3_PRODUCT_TM=67.7
PRIMER_PAIR_4_PENALTY=2.469079
PRIMER_LEFT_4_PENALTY=0.914244
PRIMER_RIGHT_4_PENALTY=1.554836
PRIMER_LEFT_4_SEQUENCE=GCAGCAAAAGAGACGATTGG
PRIMER_RIGHT_4_SEQUENCE=CCTTCTCTTCCCACCCATC
PRIMER_LEFT_4=103,20
PRIMER_RIGHT_4=231,19
PRIMER_LEFT_4_TM=60.914
PRIMER_RIGHT_4_TM=59.445
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=129
PRIMER_PAIR_4_PRODUCT_TM=67.3
=
SEQUENCE_ID=08.RHAP131FC8.seq
SEQUENCE_TEMPLATE=AAAAANNGNTTAGAGGATCCCCCATAGAAGCTTCTACTATACCAGCTCTAGTAGAAGGGGACAAGCCTTAGTTTATGCAACTGAGTTCATGGCACGGGATTGTAAATGGTACATGAGTCTTATGTTCAAGCATTATTTCTATAAAATCTCACTTTTTAAGTCCAGTGAGTAAGCTGTGAGCTCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCCCAGCTACTTGGGAGGCTCAGTTCCATGATGTTTATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,293
SEQUENCE_TARGET=187,64
P3_COMMENT=CL is TCCAGTGAGTAAGCTGTGAGCT
P3_COMMENT=CR is CATCATGGAACTGAGCCTCC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.317152
PRIMER_LEFT_0_PENALTY=2.694680
PRIMER_RIGHT_0_PENALTY=0.622472
PRIMER_LEFT_0_SEQUENCE=CCAGTGAGTAAGCTGTGAGCTC
PRIMER_RIGHT_0_SEQUENCE=CATCATGGAACTGAGCCTCC
PRIMER_LEFT_0=161,22
PRIMER_RIGHT_0=285,20
PRIMER_LEFT_0_TM=59.305
PRIMER_RIGHT_0_TM=60.622
PRIMER_LEFT_0_GC_PERCENT=54.545
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=125
PRIMER_PAIR_0_PRODUCT_TM=66.6
PRIMER_PAIR_1_PENALTY=3.317152
PRIMER_LEFT_1_PENALTY=2.694680
PRIMER_RIGHT_1_PENALTY=0.622472
PRIMER_LEFT_1_SEQUENCE=TCCAGTGAGTAAGCTGTGAGCT
PRIMER_RIGHT_1_SEQUENCE=CATCATGGAACTGAGCCTCC
PRIMER_LEFT_1=160,22
PRIMER_RIGHT_1=285,20
PRIMER_LEFT_1_TM=59.305
PRIMER_RIGHT_1_TM=60.622
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=126
PRIMER_PAIR_1_PRODUCT_TM=66.5
PRIMER_PAIR_2_PENALTY=3.364888
PRIMER_LEFT_2_PENALTY=2.742415
PRIMER_RIGHT_2_PENALTY=0.622472
PRIMER_LEFT_2_SEQUENCE=TCCAGTGAGTAAGCTGTGAGC
PRIMER_RIGHT_2_SEQUENCE=CATCATGGAACTGAGCCTCC
PRIMER_LEFT_2=160,21
PRIMER_RIGHT_2=285,20
PRIMER_LEFT_2_TM=58.258
PRIMER_RIGHT_2_TM=60.622
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=126
PRIMER_PAIR_2_PRODUCT_TM=66.5
PRIMER_PAIR_3_PENALTY=3.457555
PRIMER_LEFT_3_PENALTY=2.835083
PRIMER_RIGHT_3_PENALTY=0.622472
PRIMER_LEFT_3_SEQUENCE=GTCCAGTGAGTAAGCTGTGAGC
PRIMER_RIGHT_3_SEQUENCE=CATCATGGAACTGAGCCTCC
PRIMER_LEFT_3=159,22
PRIMER_RIGHT_3=285,20
PRIMER_LEFT_3_TM=59.165
PRIMER_RIGHT_3_TM=60.622
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=66.8
PRIMER_PAIR_4_PENALTY=3.548510
PRIMER_LEFT_4_PENALTY=1.208168
PRIMER_RIGHT_4_PENALTY=2.340342
PRIMER_LEFT_4_SEQUENCE=GTGAGTAAGCTGTGAGCTCCG
PRIMER_RIGHT_4_SEQUENCE=AAACATCATGGAACTGAGCCT
PRIMER_LEFT_4=164,21
PRIMER_RIGHT_4=288,21
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=58.660
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=125
PRIMER_PAIR_4_PRODUCT_TM=65.9
=
SEQUENCE_ID=10.RHAP131FD1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNGAACTATATCCCCAGCTCTTAGGAATGGAAATTCAGGATTCCAAAAGGAAGCAAAAAATAAAGCCTCACCCATAACCCCACATTCTTTGTGCACGCCCCTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGAGAATTGAACCCGGAGTCTGTCTAAGCTAAGTGNNNNNNNNNNNNNNGAGCCACCGCTAGCCACAGCGTTGTTTAATTACCTGTTGCTCTAGTTCAGAACTTTTACTGTGGTTAACCATGTTGAGCTACAGTGTTTCACTGTAACCACTTCTCATGGCTCGCTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=11,326
SEQUENCE_TARGET=118,46
SEQUENCE_TARGET=203,14
P3_COMMENT=CL is CAGGATTCCAAAAGGAAGCA
P3_COMMENT=CR is GACTCCGGGTTCAATTCTCA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.233803
PRIMER_LEFT_0_PENALTY=0.183944
PRIMER_RIGHT_0_PENALTY=0.049859
PRIMER_LEFT_0_SEQUENCE=CAGGATTCCAAAAGGAAGCA
PRIMER_RIGHT_0_SEQUENCE=GACTCCGGGTTCAATTCTCA
PRIMER_LEFT_0=44,20
PRIMER_RIGHT_0=187,20
PRIMER_LEFT_0_TM=60.184
PRIMER_RIGHT_0_TM=60.050
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=144
PRIMER_PAIR_0_PRODUCT_TM=69.1
PRIMER_PAIR_1_PENALTY=0.785453
PRIMER_LEFT_1_PENALTY=0.735594
PRIMER_RIGHT_1_PENALTY=0.049859
PRIMER_LEFT_1_SEQUENCE=CCCAGCTCTTAGGAATGGAA
PRIMER_RIGHT_1_SEQUENCE=GACTCCGGGTTCAATTCTCA
PRIMER_LEFT_1=21,20
PRIMER_RIGHT_1=187,20
PRIMER_LEFT_1_TM=59.264
PRIMER_RIGHT_1_TM=60.050
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=167
PRIMER_PAIR_1_PRODUCT_TM=70.3
PRIMER_PAIR_2_PENALTY=0.805400
PRIMER_LEFT_2_PENALTY=0.755541
PRIMER_RIGHT_2_PENALTY=0.049859
PRIMER_LEFT_2_SEQUENCE=TCAGGATTCCAAAAGGAAGC
PRIMER_RIGHT_2_SEQUENCE=GACTCCGGGTTCAATTCTCA
PRIMER_LEFT_2=43,20
PRIMER_RIGHT_2=187,20
PRIMER_LEFT_2_TM=59.244
PRIMER_RIGHT_2_TM=60.050
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=145
PRIMER_PAIR_2_PRODUCT_TM=69.1
PRIMER_PAIR_3_PENALTY=0.915016
PRIMER_LEFT_3_PENALTY=0.865157
PRIMER_RIGHT_3_PENALTY=0.049859
PRIMER_LEFT_3_SEQUENCE=GGATTCCAAAAGGAAGCAAA
PRIMER_RIGHT_3_SEQUENCE=GACTCCGGGTTCAATTCTCA
PRIMER_LEFT_3=46,20
PRIMER_RIGHT_3=187,20
PRIMER_LEFT_3_TM=59.135
PRIMER_RIGHT_3_TM=60.050
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=142
PRIMER_PAIR_3_PRODUCT_TM=69.0
PRIMER_PAIR_4_PENALTY=1.046923
PRIMER_LEFT_4_PENALTY=0.183944
PRIMER_RIGHT_4_PENALTY=0.862979
PRIMER_LEFT_4_SEQUENCE=CAGGATTCCAAAAGGAAGCA
PRIMER_RIGHT_4_SEQUENCE=CAGACTCCGGGTTCAATTCT
PRIMER_LEFT_4=44,20
PRIMER_RIGHT_4=189,20
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=59.137
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=146
PRIMER_PAIR_4_PRODUCT_TM=69.3
=
SEQUENCE_ID=10.RHAP131FD7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCACTACTGCAACAGTGTTTCCACTTCAGCATGTGTGGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATGGGTATCTCTATCTGTATCTGTGTATCTCCTTTATTTATTGATGACTTTAGATGCAGATTTTTTAGGGTGGAGGATGTCCTTGTTTTGATCACATGTTATTTGAAGAACACAATTCCCAGCTAAAAGAATTTAGCTATTTTAGTTGTATCTGCAATGAAAATGCAACTTTTCAGTGAAATTAGCCACGGGTTGAGTTTATGATGAGATGCTCTTTGGAAGACAGAAATGTACTCTCATTTATAAGTTCAAGTCTCATTGAGAATACTCCAAGATTTATTTGTTTACTTTTCAAGAAAAAAAATTGATTTACAGTCTCGAACACAAGAACAAAGCCACAGACAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=12,433
SEQUENCE_TARGET=53,42
P3_COMMENT=CL is TTCCACTTCAGCATGTGTGG
P3_COMMENT=CR is CAAGGACATCCTCCACCCTA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.802713
PRIMER_LEFT_0_PENALTY=0.722400
PRIMER_RIGHT_0_PENALTY=0.080313
PRIMER_LEFT_0_SEQUENCE=TTCCACTTCAGCATGTGTGG
PRIMER_RIGHT_0_SEQUENCE=CAAGGACATCCTCCACCCTA
PRIMER_LEFT_0=29,20
PRIMER_RIGHT_0=182,20
PRIMER_LEFT_0_TM=60.722
PRIMER_RIGHT_0_TM=59.920
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=154
PRIMER_PAIR_0_PRODUCT_TM=67.7
PRIMER_PAIR_1_PENALTY=1.303350
PRIMER_LEFT_1_PENALTY=1.223037
PRIMER_RIGHT_1_PENALTY=0.080313
PRIMER_LEFT_1_SEQUENCE=CACTTCAGCATGTGTGGGTG
PRIMER_RIGHT_1_SEQUENCE=CAAGGACATCCTCCACCCTA
PRIMER_LEFT_1=32,20
PRIMER_RIGHT_1=182,20
PRIMER_LEFT_1_TM=61.223
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=151
PRIMER_PAIR_1_PRODUCT_TM=67.6
PRIMER_PAIR_2_PENALTY=1.411547
PRIMER_LEFT_2_PENALTY=1.331234
PRIMER_RIGHT_2_PENALTY=0.080313
PRIMER_LEFT_2_SEQUENCE=TGCAACAGTGTTTCCACTTCA
PRIMER_RIGHT_2_SEQUENCE=CAAGGACATCCTCCACCCTA
PRIMER_LEFT_2=18,21
PRIMER_RIGHT_2=182,20
PRIMER_LEFT_2_TM=60.331
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=165
PRIMER_PAIR_2_PRODUCT_TM=68.4
PRIMER_PAIR_3_PENALTY=1.492036
PRIMER_LEFT_3_PENALTY=0.722400
PRIMER_RIGHT_3_PENALTY=0.769636
PRIMER_LEFT_3_SEQUENCE=TTCCACTTCAGCATGTGTGG
PRIMER_RIGHT_3_SEQUENCE=ACAAGGACATCCTCCACCCT
PRIMER_LEFT_3=29,20
PRIMER_RIGHT_3=183,20
PRIMER_LEFT_3_TM=60.722
PRIMER_RIGHT_3_TM=60.770
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=155
PRIMER_PAIR_3_PRODUCT_TM=67.7
PRIMER_PAIR_4_PENALTY=1.730779
PRIMER_LEFT_4_PENALTY=1.650466
PRIMER_RIGHT_4_PENALTY=0.080313
PRIMER_LEFT_4_SEQUENCE=CAGTGTTTCCACTTCAGCATG
PRIMER_RIGHT_4_SEQUENCE=CAAGGACATCCTCCACCCTA
PRIMER_LEFT_4=23,21
PRIMER_RIGHT_4=182,20
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=59.920
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=160
PRIMER_PAIR_4_PRODUCT_TM=68.2
=
SEQUENCE_ID=11.RHAP131FD2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAACCATCAACCGAAGCACAAGAACAGACGTGCGTCTTTTGGCTCGCAGTGATTTTTAATGGTAGTCATCCGGACATACGACTTAGATACAATGAGACTACAAACTAACAGACAAGATTCGGGCAAAGCAGAAAAACAATGAAGCCAGCATCCTGATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGCATGCCTGCCTGTCAATTGTGAGGTAAGACAGGAAGACTTGGTCTCCTGCCAGATCTCCATATCTTCCCGCTCTCCTTTAACTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=22,297
SEQUENCE_TARGET=183,45
P3_COMMENT=CL is AAACAATGAAGCCAGCATCC
P3_COMMENT=CR is TCTGGCAGGAGACCAAGTCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.095381
PRIMER_LEFT_0_PENALTY=0.080962
PRIMER_RIGHT_0_PENALTY=0.014419
PRIMER_LEFT_0_SEQUENCE=AAACAATGAAGCCAGCATCC
PRIMER_RIGHT_0_SEQUENCE=TCTGGCAGGAGACCAAGTCT
PRIMER_LEFT_0=156,20
PRIMER_RIGHT_0=285,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=59.986
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=130
PRIMER_PAIR_0_PRODUCT_TM=68.2
PRIMER_PAIR_1_PENALTY=0.203165
PRIMER_LEFT_1_PENALTY=0.188746
PRIMER_RIGHT_1_PENALTY=0.014419
PRIMER_LEFT_1_SEQUENCE=ATGAAGCCAGCATCCTGATC
PRIMER_RIGHT_1_SEQUENCE=TCTGGCAGGAGACCAAGTCT
PRIMER_LEFT_1=161,20
PRIMER_RIGHT_1=285,20
PRIMER_LEFT_1_TM=60.189
PRIMER_RIGHT_1_TM=59.986
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_1_PRODUCT_TM=68.2
PRIMER_PAIR_2_PENALTY=0.314662
PRIMER_LEFT_2_PENALTY=0.300243
PRIMER_RIGHT_2_PENALTY=0.014419
PRIMER_LEFT_2_SEQUENCE=AACAATGAAGCCAGCATCCT
PRIMER_RIGHT_2_SEQUENCE=TCTGGCAGGAGACCAAGTCT
PRIMER_LEFT_2=157,20
PRIMER_RIGHT_2=285,20
PRIMER_LEFT_2_TM=59.700
PRIMER_RIGHT_2_TM=59.986
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=129
PRIMER_PAIR_2_PRODUCT_TM=68.3
PRIMER_PAIR_3_PENALTY=0.355941
PRIMER_LEFT_3_PENALTY=0.341522
PRIMER_RIGHT_3_PENALTY=0.014419
PRIMER_LEFT_3_SEQUENCE=AATGAAGCCAGCATCCTGAT
PRIMER_RIGHT_3_SEQUENCE=TCTGGCAGGAGACCAAGTCT
PRIMER_LEFT_3=160,20
PRIMER_RIGHT_3=285,20
PRIMER_LEFT_3_TM=59.658
PRIMER_RIGHT_3_TM=59.986
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=126
PRIMER_PAIR_3_PRODUCT_TM=68.2
PRIMER_PAIR_4_PENALTY=0.430002
PRIMER_LEFT_4_PENALTY=0.080962
PRIMER_RIGHT_4_PENALTY=0.349041
PRIMER_LEFT_4_SEQUENCE=AAACAATGAAGCCAGCATCC
PRIMER_RIGHT_4_SEQUENCE=GAGATCTGGCAGGAGACCAA
PRIMER_LEFT_4=156,20
PRIMER_RIGHT_4=289,20
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.349
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=134
PRIMER_PAIR_4_PRODUCT_TM=68.6
=
SEQUENCE_ID=13.RHAP131FE1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCATGCCTGGAAGCCACATTGACTTTATATTGAAATAATTCTTTTTTAAAAAGTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCGCATGTGTGCTCATGCCCTTGTGTGCATGATAAGTGTATGTAGGCTTTTGTGCCACAGCAAATGTGTAGAAGTCAAAGGAAAACATTCTGAAACGAGTTCTATCTACTTTTCTGTGGATTCCAGGGACTGAACCTGTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,241
SEQUENCE_TARGET=77,38
P3_COMMENT=CL is CCTGGAAGCCACATTGACTT
P3_COMMENT=CR is TATCATGCACACAAGGGCAT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.155829
PRIMER_LEFT_0_PENALTY=0.111077
PRIMER_RIGHT_0_PENALTY=0.044752
PRIMER_LEFT_0_SEQUENCE=CCTGGAAGCCACATTGACTT
PRIMER_RIGHT_0_SEQUENCE=TATCATGCACACAAGGGCAT
PRIMER_LEFT_0=25,20
PRIMER_RIGHT_0=151,20
PRIMER_LEFT_0_TM=60.111
PRIMER_RIGHT_0_TM=59.955
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=127
PRIMER_PAIR_0_PRODUCT_TM=65.8
PRIMER_PAIR_1_PENALTY=0.206583
PRIMER_LEFT_1_PENALTY=0.111077
PRIMER_RIGHT_1_PENALTY=0.095506
PRIMER_LEFT_1_SEQUENCE=CCTGGAAGCCACATTGACTT
PRIMER_RIGHT_1_SEQUENCE=CTGTGGCACAAAAGCCTACA
PRIMER_LEFT_1=25,20
PRIMER_RIGHT_1=177,20
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=153
PRIMER_PAIR_1_PRODUCT_TM=68.0
PRIMER_PAIR_2_PENALTY=0.268317
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=0.157240
PRIMER_LEFT_2_SEQUENCE=CCTGGAAGCCACATTGACTT
PRIMER_RIGHT_2_SEQUENCE=ACATTTGCTGTGGCACAAAA
PRIMER_LEFT_2=25,20
PRIMER_RIGHT_2=184,20
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=60.157
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=160
PRIMER_PAIR_2_PRODUCT_TM=68.2
PRIMER_PAIR_3_PENALTY=0.404572
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.293496
PRIMER_LEFT_3_SEQUENCE=CCTGGAAGCCACATTGACTT
PRIMER_RIGHT_3_SEQUENCE=CATTTGCTGTGGCACAAAAG
PRIMER_LEFT_3=25,20
PRIMER_RIGHT_3=183,20
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=60.293
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=159
PRIMER_PAIR_3_PRODUCT_TM=68.2
PRIMER_PAIR_4_PENALTY=0.912165
PRIMER_LEFT_4_PENALTY=0.111077
PRIMER_RIGHT_4_PENALTY=0.801088
PRIMER_LEFT_4_SEQUENCE=CCTGGAAGCCACATTGACTT
PRIMER_RIGHT_4_SEQUENCE=TGTGGCACAAAAGCCTACAT
PRIMER_LEFT_4=25,20
PRIMER_RIGHT_4=176,20
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=59.199
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=152
PRIMER_PAIR_4_PRODUCT_TM=67.8
=
SEQUENCE_ID=13.RHAP131FE7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCTTTTCAAATGTGGAGCCTACTCTAACTTTCAGTCCCTGGGTCTCTCTCAAATATTTTCNNNNNNNNNNNNNNNNNNNNNNGTTTTTNNNNNNNNNNNNNNNNNNNNNNNGNNTNNNNCTCTCNNNNNNNCNCNCNNNNNNNNNANNTCNCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAGNNNAAANAANCAANAGANCCCNAGAAAAAGTGCACATCNATGCCAACATACAGNAAAAAAAACATTTNGACACACANAAAGAATANAAAACANANNTGTGNNNNATCAANGGTATTTTNGAACCNAACACGAATGNGTGANCANNGNNCANNANCGNTGCTCCNNTGTGAAATTGTTANCAGCTCANTNTTCCTGCNGTAATAATANNTACCCGCAGAATNNTNTACAAAACATGGGGGNCGNAAACATATGNGTNTTACANNCNGGNGTGGCTNNNGNGTGAGCTNACANANNNNNGGNGGGGNAGCGCTCTCNGGNCANCTGCCTNNNGGNANNNNCANCGCGCCNGNANATGNAGNANNCGAANNCGCGNNGGNANNNNNTGTCTNNNAANAGGNANAAGGGGANNNCTTNTTTCCCANAGNGGCNGGCNNNANANNTNNNNNACCGGCCACACTGANNTCCCAAANNNC
SEQUENCE_INCLUDED_REGION=27,677
SEQUENCE_TARGET=90,22
SEQUENCE_TARGET=118,22
SEQUENCE_TARGET=189,36
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=14.RHAP131FE8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCCAGGAACCCAGCCAGTTTTCAAAGAATACTTACCATGCCATACAAGTCAGAACCTGAACCTAGGCTTTCAAGCTGTGTAAATCAATGTTCAATTGACTGTAGTAGGAGACCCACAGACTCTATTAGAAGCATTGTAGGACAATGCTAGATTTTCTGTTCTAATGTTCTTCAGATTTAGAACAACGATGACAACAGCATTAGTGTAATAGTGTTAACTGAGTACTGTAGTGCAAGAGTGCCTGCATTCCCACATACAGTATTCATAGCTTTTGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCAGTTTAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=25,321
SEQUENCE_TARGET=301,32
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=15.RHAP131FE3.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCTGTTTGACAGCAGGCAGAAGCTCAGGAACAATTTGTGTGGGAGTCCCAGAGGGGAGCAGGGTGACTGAAAATCCTCTGGGCTGTTACAGAACGGAGTTTGGAATCAAAGTAACCAGAACATTCAGCTCAGTAAGAGCTTAAAAGGACATTTCATCCCCCTTAAAGACAGCTAATAAAAATAGATTTGCAGTCAGTTTTCATTAGCCAAAGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCCGCGTGCATAGGGTGTCTTCCTCAGCTGCTCTGCTCACCTTATGTTTTGAGACAGTGTCTGTCTTTGAACCTGTAGTTCAGCTTTTTCTACTAGGCTGGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=12,368
SEQUENCE_TARGET=229,38
P3_COMMENT=CL is AAAAGGACATTTCATCCCCC
P3_COMMENT=CR is AGGAAGACACCCTATGCACG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.137982
PRIMER_LEFT_0_PENALTY=0.004242
PRIMER_RIGHT_0_PENALTY=0.133741
PRIMER_LEFT_0_SEQUENCE=AAAAGGACATTTCATCCCCC
PRIMER_RIGHT_0_SEQUENCE=AGGAAGACACCCTATGCACG
PRIMER_LEFT_0=153,20
PRIMER_RIGHT_0=298,20
PRIMER_LEFT_0_TM=59.996
PRIMER_RIGHT_0_TM=60.134
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=146
PRIMER_PAIR_0_PRODUCT_TM=69.8
PRIMER_PAIR_1_PENALTY=0.147644
PRIMER_LEFT_1_PENALTY=0.004242
PRIMER_RIGHT_1_PENALTY=0.143403
PRIMER_LEFT_1_SEQUENCE=AAAAGGACATTTCATCCCCC
PRIMER_RIGHT_1_SEQUENCE=GAGCAGCTGAGGAAGACACC
PRIMER_LEFT_1=153,20
PRIMER_RIGHT_1=307,20
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.143
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=155
PRIMER_PAIR_1_PRODUCT_TM=70.8
PRIMER_PAIR_2_PENALTY=0.444387
PRIMER_LEFT_2_PENALTY=0.004242
PRIMER_RIGHT_2_PENALTY=0.440146
PRIMER_LEFT_2_SEQUENCE=AAAAGGACATTTCATCCCCC
PRIMER_RIGHT_2_SEQUENCE=GCAGAGCAGCTGAGGAAGAC
PRIMER_LEFT_2=153,20
PRIMER_RIGHT_2=310,20
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.440
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=71.2
PRIMER_PAIR_3_PENALTY=0.505105
PRIMER_LEFT_3_PENALTY=0.371364
PRIMER_RIGHT_3_PENALTY=0.133741
PRIMER_LEFT_3_SEQUENCE=AAGGACATTTCATCCCCCTT
PRIMER_RIGHT_3_SEQUENCE=AGGAAGACACCCTATGCACG
PRIMER_LEFT_3=155,20
PRIMER_RIGHT_3=298,20
PRIMER_LEFT_3_TM=59.629
PRIMER_RIGHT_3_TM=60.134
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=144
PRIMER_PAIR_3_PRODUCT_TM=70.0
PRIMER_PAIR_4_PENALTY=0.505105
PRIMER_LEFT_4_PENALTY=0.371364
PRIMER_RIGHT_4_PENALTY=0.133741
PRIMER_LEFT_4_SEQUENCE=AAAGGACATTTCATCCCCCT
PRIMER_RIGHT_4_SEQUENCE=AGGAAGACACCCTATGCACG
PRIMER_LEFT_4=154,20
PRIMER_RIGHT_4=298,20
PRIMER_LEFT_4_TM=59.629
PRIMER_RIGHT_4_TM=60.134
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=145
PRIMER_PAIR_4_PRODUCT_TM=69.9
=
SEQUENCE_ID=15.RHAP131FE9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTCCATTGTGAGACACTCATTGTTGGACTAGCCCAACCATAGCCTGCAAGCCATTCTAATAAAAATTGCTTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCATGAGCAGGAGGATGGAAACATGATGCCCTTAATTCAATGAAGTCTAAACAATATATAGACAGTTGCTATCAAGCTCTAGGAAAACAATCAGCACGACTTATTTGGGAATAAATAGATCACAAGAGTCTCTTGAGTCAACTTACAAGAGTCCCTAGCCCATGTTTCATGGATGCTGCACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=26,309
SEQUENCE_TARGET=104,46
P3_COMMENT=CL is TGGACTAGCCCAACCATAGC
P3_COMMENT=CR is AAGGGCATCATGTTTCCATC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.339571
PRIMER_LEFT_0_PENALTY=0.095771
PRIMER_RIGHT_0_PENALTY=0.243800
PRIMER_LEFT_0_SEQUENCE=TGGACTAGCCCAACCATAGC
PRIMER_RIGHT_0_SEQUENCE=AAGGGCATCATGTTTCCATC
PRIMER_LEFT_0=52,20
PRIMER_RIGHT_0=183,20
PRIMER_LEFT_0_TM=60.096
PRIMER_RIGHT_0_TM=59.756
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.1000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=66.8
PRIMER_PAIR_1_PENALTY=0.385209
PRIMER_LEFT_1_PENALTY=0.095771
PRIMER_RIGHT_1_PENALTY=0.289438
PRIMER_LEFT_1_SEQUENCE=TGGACTAGCCCAACCATAGC
PRIMER_RIGHT_1_SEQUENCE=GCATCATGTTTCCATCCTCC
PRIMER_LEFT_1=52,20
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.289
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=128
PRIMER_PAIR_1_PRODUCT_TM=66.4
PRIMER_PAIR_2_PENALTY=0.385209
PRIMER_LEFT_2_PENALTY=0.095771
PRIMER_RIGHT_2_PENALTY=0.289438
PRIMER_LEFT_2_SEQUENCE=TGGACTAGCCCAACCATAGC
PRIMER_RIGHT_2_SEQUENCE=GGCATCATGTTTCCATCCTC
PRIMER_LEFT_2=52,20
PRIMER_RIGHT_2=180,20
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.289
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=129
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=0.448041
PRIMER_LEFT_3_PENALTY=0.375090
PRIMER_RIGHT_3_PENALTY=0.072951
PRIMER_LEFT_3_SEQUENCE=TGTTGGACTAGCCCAACCAT
PRIMER_RIGHT_3_SEQUENCE=CATGTTTCCATCCTCCTGCT
PRIMER_LEFT_3=49,20
PRIMER_RIGHT_3=175,20
PRIMER_LEFT_3_TM=60.375
PRIMER_RIGHT_3_TM=60.073
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=66.2
PRIMER_PAIR_4_PENALTY=0.618890
PRIMER_LEFT_4_PENALTY=0.375090
PRIMER_RIGHT_4_PENALTY=0.243800
PRIMER_LEFT_4_SEQUENCE=TGTTGGACTAGCCCAACCAT
PRIMER_RIGHT_4_SEQUENCE=AAGGGCATCATGTTTCCATC
PRIMER_LEFT_4=49,20
PRIMER_RIGHT_4=183,20
PRIMER_LEFT_4_TM=60.375
PRIMER_RIGHT_4_TM=59.756
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=135
PRIMER_PAIR_4_PRODUCT_TM=67.0
=
SEQUENCE_ID=16.RHAP131FF1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNCCACGAATCAAAGAAAAGGCAAACTAGGGAAAGAAAAGCTAGTCTCAAACACCTGACCAATGGAAACCTTAGAAAGAGTGAGAGCCAAAATGATGCAGTTCCCAGTACTCCCTTATTGAAGATGATCTAGTTCCAAGCACTCACTACTCATAAATACAGTACCTGGTTCTCCCCTATCCAATGTAATACAGTACTTAGTACTCCCCCACCCAGTGATATAGTGCTTAGTACTCAGCCATGATGAGAGTACCTAACACTTCCCCCACCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGNGGGGGTGNNTNTGTNNGNNTCNGGGGGTNNNGCGCGCNNNNNNGNGNAGCATGGNGNNANNNGTCTNNTGATGTGAAANNNTATNNNCGCACAATNNCTGCANAACANANGAGNNGGNGNGNNAAATGNGGTGAAANCNGNGGNGCCNANTGAGNGAGNNNCACACANTAANANNGNGGNGCNNNNNGGCGCNNTGNANNNGGGGNANNNNNNGTGNNANCTGNACTGNNNGNNNGGCNTNNNGGNNGGNG
SEQUENCE_INCLUDED_REGION=11,569
SEQUENCE_TARGET=283,40
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=16.RHAP131FF7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCCCTTAAATGCATCACGAGAATTCTCACTTGAGATGATTTCAATTCTAGCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTGTTCCGGCGCGCACACACGCACATATACAGCAAAATATAACTGAATGCCAAAAAGCATTGTCATGTCCTGTGAAGGGCACAATTGTAATCACACAGGTATGAAAAGCTTAAAATCCTTCCATTACCTTCCATGCCTGAACACTGACAGGGTCGGGCTCAGGAGCAGAGCCCACTTCCTGAGCTATTTATTTGTATCTCTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=12,305
SEQUENCE_TARGET=66,44
P3_COMMENT=CL is CCCCTTAAATGCATCACGAG
P3_COMMENT=CR is TGTATATGTGCGTGTGTGCG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.668814
PRIMER_LEFT_0_PENALTY=0.460175
PRIMER_RIGHT_0_PENALTY=0.208639
PRIMER_LEFT_0_SEQUENCE=CCCCTTAAATGCATCACGAG
PRIMER_RIGHT_0_SEQUENCE=TGTATATGTGCGTGTGTGCG
PRIMER_LEFT_0=12,20
PRIMER_RIGHT_0=142,20
PRIMER_LEFT_0_TM=60.460
PRIMER_RIGHT_0_TM=60.209
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=131
PRIMER_PAIR_0_PRODUCT_TM=68.2
PRIMER_PAIR_1_PENALTY=1.104173
PRIMER_LEFT_1_PENALTY=0.460175
PRIMER_RIGHT_1_PENALTY=0.643998
PRIMER_LEFT_1_SEQUENCE=CCCCTTAAATGCATCACGAG
PRIMER_RIGHT_1_SEQUENCE=CATGACAATGCTTTTTGGCA
PRIMER_LEFT_1=12,20
PRIMER_RIGHT_1=178,20
PRIMER_LEFT_1_TM=60.460
PRIMER_RIGHT_1_TM=60.644
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=167
PRIMER_PAIR_1_PRODUCT_TM=69.3
PRIMER_PAIR_2_PENALTY=1.159164
PRIMER_LEFT_2_PENALTY=0.460175
PRIMER_RIGHT_2_PENALTY=0.698990
PRIMER_LEFT_2_SEQUENCE=CCCCTTAAATGCATCACGAG
PRIMER_RIGHT_2_SEQUENCE=CAATGCTTTTTGGCATTCAG
PRIMER_LEFT_2=12,20
PRIMER_RIGHT_2=173,20
PRIMER_LEFT_2_TM=60.460
PRIMER_RIGHT_2_TM=59.301
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=162
PRIMER_PAIR_2_PRODUCT_TM=69.1
PRIMER_PAIR_3_PENALTY=1.290037
PRIMER_LEFT_3_PENALTY=1.081398
PRIMER_RIGHT_3_PENALTY=0.208639
PRIMER_LEFT_3_SEQUENCE=CCCTTAAATGCATCACGAGAA
PRIMER_RIGHT_3_SEQUENCE=TGTATATGTGCGTGTGTGCG
PRIMER_LEFT_3=13,21
PRIMER_RIGHT_3=142,20
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=130
PRIMER_PAIR_3_PRODUCT_TM=67.9
PRIMER_PAIR_4_PENALTY=1.299052
PRIMER_LEFT_4_PENALTY=0.460175
PRIMER_RIGHT_4_PENALTY=0.838878
PRIMER_LEFT_4_SEQUENCE=CCCCTTAAATGCATCACGAG
PRIMER_RIGHT_4_SEQUENCE=ACAATGCTTTTTGGCATTCA
PRIMER_LEFT_4=12,20
PRIMER_RIGHT_4=174,20
PRIMER_LEFT_4_TM=60.460
PRIMER_RIGHT_4_TM=59.161
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=35.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=163
PRIMER_PAIR_4_PRODUCT_TM=69.1
=
SEQUENCE_ID=18.RHAP131FF9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCCGGTTGCATGCATGTACCCGCCCAGGAGCTAAGTCTGATTTTCGGATAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCTCATTTGAAAACTAGCTTATTGGCTCTGAGGCTGGGCTCTTCTAAGCCTTCCACAGCTAGTGTGCCCTGAGCTTCACTCCTAGCTCAATCCCTCAGTTTAGTTGCCTAGTTACCTTCTGTGAAGCCCATTGGTCTTGTTACCCAAGATGCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=16,274
SEQUENCE_TARGET=69,58
P3_COMMENT=CL is GCCCAGGAGCTAAGTCTGATT
P3_COMMENT=CR is CCAGCCTCAGAGCCAATAAG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.166815
PRIMER_LEFT_0_PENALTY=1.137562
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_LEFT_0_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
PRIMER_RIGHT_0_SEQUENCE=CCAGCCTCAGAGCCAATAAG
PRIMER_LEFT_0=36,21
PRIMER_RIGHT_0=167,20
PRIMER_LEFT_0_TM=59.862
PRIMER_RIGHT_0_TM=59.971
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=5.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=132
PRIMER_PAIR_0_PRODUCT_TM=65.3
PRIMER_PAIR_1_PENALTY=1.194449
PRIMER_LEFT_1_PENALTY=1.137562
PRIMER_RIGHT_1_PENALTY=0.056887
PRIMER_LEFT_1_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
PRIMER_RIGHT_1_SEQUENCE=AGTGAAGCTCAGGGCACACT
PRIMER_LEFT_1=36,21
PRIMER_RIGHT_1=210,20
PRIMER_LEFT_1_TM=59.862
PRIMER_RIGHT_1_TM=60.057
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=175
PRIMER_PAIR_1_PRODUCT_TM=69.4
PRIMER_PAIR_2_PENALTY=1.254144
PRIMER_LEFT_2_PENALTY=1.137562
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
PRIMER_RIGHT_2_SEQUENCE=AAGGCTTAGAAGAGCCCAGC
PRIMER_LEFT_2=36,21
PRIMER_RIGHT_2=182,20
PRIMER_LEFT_2_TM=59.862
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=147
PRIMER_PAIR_2_PRODUCT_TM=66.7
PRIMER_PAIR_3_PENALTY=1.255379
PRIMER_LEFT_3_PENALTY=1.137562
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
PRIMER_RIGHT_3_SEQUENCE=CAGCCTCAGAGCCAATAAGC
PRIMER_LEFT_3=36,21
PRIMER_RIGHT_3=166,20
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=131
PRIMER_PAIR_3_PRODUCT_TM=65.0
PRIMER_PAIR_4_PENALTY=1.301690
PRIMER_LEFT_4_PENALTY=1.137562
PRIMER_RIGHT_4_PENALTY=0.164128
PRIMER_LEFT_4_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
PRIMER_RIGHT_4_SEQUENCE=GAAGCTCAGGGCACACTAGC
PRIMER_LEFT_4=36,21
PRIMER_RIGHT_4=207,20
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=60.164
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=69.3
=
SEQUENCE_ID=19.RHAP131FG1.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNCCAGGATTGATTGACGCCTTGTGATGATGTCCTCTTCTAACCCTGCTCTTATACAAGGTGGAGTAGCTGGTATGTTTGAATGTTTAAATAAAAGGTACCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCTTGGTATAGGTGCTTATAACTGTATCTCAGGTCCTCTGGGTTTGACAGTCAACATAACAGCCATGACAGCCCTGTCCACTAGGGCACTTCTATCAGTTCTGATATCACAGTGGAGCCAAACATGGACTGTTAGCATTCTATGCTCCACCCAGTGTTACCTAGCAACAACCAGGCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=11,331
SEQUENCE_TARGET=113,46
P3_COMMENT=CL is CCCTGCTCTTATACAAGGTGG
P3_COMMENT=CR is GTCAAACCCAGAGGACCTGA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.358596
PRIMER_LEFT_0_PENALTY=2.270426
PRIMER_RIGHT_0_PENALTY=0.088170
PRIMER_LEFT_0_SEQUENCE=CCCTGCTCTTATACAAGGTGG
PRIMER_RIGHT_0_SEQUENCE=GTCAAACCCAGAGGACCTGA
PRIMER_LEFT_0=51,21
PRIMER_RIGHT_0=209,20
PRIMER_LEFT_0_TM=58.730
PRIMER_RIGHT_0_TM=60.088
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=159
PRIMER_PAIR_0_PRODUCT_TM=68.2
PRIMER_PAIR_1_PENALTY=2.499607
PRIMER_LEFT_1_PENALTY=2.270426
PRIMER_RIGHT_1_PENALTY=0.229181
PRIMER_LEFT_1_SEQUENCE=CCCTGCTCTTATACAAGGTGG
PRIMER_RIGHT_1_SEQUENCE=CAAACCCAGAGGACCTGAGA
PRIMER_LEFT_1=51,21
PRIMER_RIGHT_1=207,20
PRIMER_LEFT_1_TM=58.730
PRIMER_RIGHT_1_TM=60.229
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=157
PRIMER_PAIR_1_PRODUCT_TM=68.1
PRIMER_PAIR_2_PENALTY=2.499607
PRIMER_LEFT_2_PENALTY=2.270426
PRIMER_RIGHT_2_PENALTY=0.229181
PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTTATACAAGGTGG
PRIMER_RIGHT_2_SEQUENCE=TCAAACCCAGAGGACCTGAG
PRIMER_LEFT_2=51,21
PRIMER_RIGHT_2=208,20
PRIMER_LEFT_2_TM=58.730
PRIMER_RIGHT_2_TM=60.229
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=158
PRIMER_PAIR_2_PRODUCT_TM=68.0
PRIMER_PAIR_3_PENALTY=2.545053
PRIMER_LEFT_3_PENALTY=2.456883
PRIMER_RIGHT_3_PENALTY=0.088170
PRIMER_LEFT_3_SEQUENCE=ACCCTGCTCTTATACAAGGTGG
PRIMER_RIGHT_3_SEQUENCE=GTCAAACCCAGAGGACCTGA
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=209,20
PRIMER_LEFT_3_TM=59.543
PRIMER_RIGHT_3_TM=60.088
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=160
PRIMER_PAIR_3_PRODUCT_TM=68.2
PRIMER_PAIR_4_PENALTY=2.550792
PRIMER_LEFT_4_PENALTY=2.462622
PRIMER_RIGHT_4_PENALTY=0.088170
PRIMER_LEFT_4_SEQUENCE=TGTCCTCTTCTAACCCTGCTC
PRIMER_RIGHT_4_SEQUENCE=GTCAAACCCAGAGGACCTGA
PRIMER_LEFT_4=38,21
PRIMER_RIGHT_4=209,20
PRIMER_LEFT_4_TM=58.537
PRIMER_RIGHT_4_TM=60.088
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=172
PRIMER_PAIR_4_PRODUCT_TM=68.8
=
SEQUENCE_ID=19.RHAP131FG7.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCATGGATAACAGGGATGGACAACAAGGAAGAGCTACAGAGACATCCAGACAGCCATTATCTGCCTCATACTATCCCAACTGTTACACTTCCTATCCATACCTGCTGCTTTCAAAACCAAGTCTAGCCCCTTCTGAGAATTATTTAGGGGTGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAGAACAACTTGCAGAAGAAGGCTCTTTCTTACCTTCCACCACATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=14,258
SEQUENCE_TARGET=171,52
P3_COMMENT=CL is CCAAGTCTAGCCCCTTCTGA
P3_COMMENT=CR is CCATGTGGTGGAAGGTAAGAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.737208
PRIMER_LEFT_0_PENALTY=0.575355
PRIMER_RIGHT_0_PENALTY=1.161853
PRIMER_LEFT_0_SEQUENCE=CCAAGTCTAGCCCCTTCTGA
PRIMER_RIGHT_0_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
PRIMER_LEFT_0=130,20
PRIMER_RIGHT_0=271,21
PRIMER_LEFT_0_TM=59.425
PRIMER_RIGHT_0_TM=59.838
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=142
PRIMER_PAIR_0_PRODUCT_TM=68.1
PRIMER_PAIR_1_PENALTY=1.742019
PRIMER_LEFT_1_PENALTY=0.580167
PRIMER_RIGHT_1_PENALTY=1.161853
PRIMER_LEFT_1_SEQUENCE=TCCATACCTGCTGCTTTCAA
PRIMER_RIGHT_1_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
PRIMER_LEFT_1=108,20
PRIMER_RIGHT_1=271,21
PRIMER_LEFT_1_TM=59.420
PRIMER_RIGHT_1_TM=59.838
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=164
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=1.968469
PRIMER_LEFT_2_PENALTY=0.806616
PRIMER_RIGHT_2_PENALTY=1.161853
PRIMER_LEFT_2_SEQUENCE=ACCTGCTGCTTTCAAAACCA
PRIMER_RIGHT_2_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
PRIMER_LEFT_2=113,20
PRIMER_RIGHT_2=271,21
PRIMER_LEFT_2_TM=60.807
PRIMER_RIGHT_2_TM=59.838
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=159
PRIMER_PAIR_2_PRODUCT_TM=69.0
PRIMER_PAIR_3_PENALTY=1.991414
PRIMER_LEFT_3_PENALTY=0.829561
PRIMER_RIGHT_3_PENALTY=1.161853
PRIMER_LEFT_3_SEQUENCE=TCAAAACCAAGTCTAGCCCC
PRIMER_RIGHT_3_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
PRIMER_LEFT_3=124,20
PRIMER_RIGHT_3=271,21
PRIMER_LEFT_3_TM=59.170
PRIMER_RIGHT_3_TM=59.838
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=148
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=2.161329
PRIMER_LEFT_4_PENALTY=0.575355
PRIMER_RIGHT_4_PENALTY=1.585973
PRIMER_LEFT_4_SEQUENCE=CCAAGTCTAGCCCCTTCTGA
PRIMER_RIGHT_4_SEQUENCE=CCATGTGGTGGAAGGTAAGA
PRIMER_LEFT_4=130,20
PRIMER_RIGHT_4=271,20
PRIMER_LEFT_4_TM=59.425
PRIMER_RIGHT_4_TM=58.414
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=142
PRIMER_PAIR_4_PRODUCT_TM=68.1
=
SEQUENCE_ID=20.RHAP131FG2.seq
SEQUENCE_TEMPLATE=CCGGGTTTTTGTGTATGCTTGGGTGTGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTTGTATTTGTGTTATANNNNNNNNNNNNNTTTGCATGTGCGTGTGTGAGTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,149
SEQUENCE_TARGET=31,60
SEQUENCE_TARGET=111,13
P3_COMMENT=CL is CCGGGTTTTTGTGTATGCTT
P3_COMMENT=CR is GACACTCACACACGCACATG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.367549
PRIMER_LEFT_0_PENALTY=0.139129
PRIMER_RIGHT_0_PENALTY=0.228420
PRIMER_LEFT_0_SEQUENCE=CCGGGTTTTTGTGTATGCTT
PRIMER_RIGHT_0_SEQUENCE=GACACTCACACACGCACATG
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=147,20
PRIMER_LEFT_0_TM=59.861
PRIMER_RIGHT_0_TM=59.772
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=148
PRIMER_PAIR_0_PRODUCT_TM=64.4
PRIMER_PAIR_1_PENALTY=0.388170
PRIMER_LEFT_1_PENALTY=0.159750
PRIMER_RIGHT_1_PENALTY=0.228420
PRIMER_LEFT_1_SEQUENCE=TGTATGCTTGGGTGTGGGTA
PRIMER_RIGHT_1_SEQUENCE=GACACTCACACACGCACATG
PRIMER_LEFT_1=11,20
PRIMER_RIGHT_1=147,20
PRIMER_LEFT_1_TM=59.840
PRIMER_RIGHT_1_TM=59.772
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=137
PRIMER_PAIR_1_PRODUCT_TM=63.0
PRIMER_PAIR_2_PENALTY=0.655200
PRIMER_LEFT_2_PENALTY=0.426779
PRIMER_RIGHT_2_PENALTY=0.228420
PRIMER_LEFT_2_SEQUENCE=TTGTGTATGCTTGGGTGTGG
PRIMER_RIGHT_2_SEQUENCE=GACACTCACACACGCACATG
PRIMER_LEFT_2=8,20
PRIMER_RIGHT_2=147,20
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.772
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=140
PRIMER_PAIR_2_PRODUCT_TM=63.2
PRIMER_PAIR_3_PENALTY=0.928284
PRIMER_LEFT_3_PENALTY=0.699863
PRIMER_RIGHT_3_PENALTY=0.228420
PRIMER_LEFT_3_SEQUENCE=GTGTATGCTTGGGTGTGGGT
PRIMER_RIGHT_3_SEQUENCE=GACACTCACACACGCACATG
PRIMER_LEFT_3=10,20
PRIMER_RIGHT_3=147,20
PRIMER_LEFT_3_TM=60.700
PRIMER_RIGHT_3_TM=59.772
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=138
PRIMER_PAIR_3_PRODUCT_TM=63.3
PRIMER_PAIR_4_PENALTY=1.144955
PRIMER_LEFT_4_PENALTY=0.916535
PRIMER_RIGHT_4_PENALTY=0.228420
PRIMER_LEFT_4_SEQUENCE=CGGGTTTTTGTGTATGCTTG
PRIMER_RIGHT_4_SEQUENCE=GACACTCACACACGCACATG
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=147,20
PRIMER_LEFT_4_TM=59.083
PRIMER_RIGHT_4_TM=59.772
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=147
PRIMER_PAIR_4_PRODUCT_TM=64.2
=
SEQUENCE_ID=20.RHAP131FG8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCCGTGGTGGTTCTATTTGCAGCCCTAGCATCTTCCCAGAAGAGGCGTTGAAATGAAGGGGCTACTGAGCTGGCTCAGCAGGAAAAGGCACTTAATATACAACCTGGCAATGCAAAATTCAATCCTAGGAACTCACGTGAGAGGTGGAAGGAGAGAACCAACTCCACAAACTAGTCCTCCAGCCTCCACACATGTGCCCCACCCCCAACTCACATATATAACTACGTCATTAACGTTTTTTTAAATTGTCTTAAGTGACAGCTGTGTGTCTTGACACACTCCTGTAATCCTGGCATGAGAAGCAGGAGTTCGAGGCCACAGCAGCTGACTTTGTCCATCTGCAGACCTTTTAACTCTTCAAGCTGAAGGTGTGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCACCTTTCCTTGTGGATTTGGATTTGGAGTTTCCACCTGTCTTTAGTTAATACATTCCCATTGACCATTCTTTTTTAACATTGGTTGTACCAGAGACAACGGGTAGACATCATTTCCTGTGCTGCAAATAGGNGGAACTCCTAGAGCTTTATTGNCTCTGGNNTGTGNANAGNNTGNACTNGAGAACACAAAGNATGTCAATTTNTAGAATNTGACAATTTTAGNGTCNAACATGGGTTGTGT
SEQUENCE_INCLUDED_REGION=15,666
SEQUENCE_TARGET=391,44
P3_COMMENT=CL is GGCATGAGAAGCAGGAGTTC
P3_COMMENT=CR is ATCCACAAGGAAAGGTGCAC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.069264
PRIMER_LEFT_0_PENALTY=0.042732
PRIMER_RIGHT_0_PENALTY=0.026532
PRIMER_LEFT_0_SEQUENCE=GGCATGAGAAGCAGGAGTTC
PRIMER_RIGHT_0_SEQUENCE=ATCCACAAGGAAAGGTGCAC
PRIMER_LEFT_0=305,20
PRIMER_RIGHT_0=454,20
PRIMER_LEFT_0_TM=59.957
PRIMER_RIGHT_0_TM=59.973
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=150
PRIMER_PAIR_0_PRODUCT_TM=70.7
PRIMER_PAIR_1_PENALTY=0.139587
PRIMER_LEFT_1_PENALTY=0.042732
PRIMER_RIGHT_1_PENALTY=0.096855
PRIMER_LEFT_1_SEQUENCE=GGCATGAGAAGCAGGAGTTC
PRIMER_RIGHT_1_SEQUENCE=TCCAAATCCACAAGGAAAGG
PRIMER_LEFT_1=305,20
PRIMER_RIGHT_1=459,20
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.903
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=155
PRIMER_PAIR_1_PRODUCT_TM=70.8
PRIMER_PAIR_2_PENALTY=0.170536
PRIMER_LEFT_2_PENALTY=0.008461
PRIMER_RIGHT_2_PENALTY=0.162074
PRIMER_LEFT_2_SEQUENCE=GCTGACTTTGTCCATCTGCA
PRIMER_RIGHT_2_SEQUENCE=CCAAATCCAAATCCACAAGG
PRIMER_LEFT_2=337,20
PRIMER_RIGHT_2=464,20
PRIMER_LEFT_2_TM=59.992
PRIMER_RIGHT_2_TM=60.162
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=128
PRIMER_PAIR_2_PRODUCT_TM=67.7
PRIMER_PAIR_3_PENALTY=0.204806
PRIMER_LEFT_3_PENALTY=0.042732
PRIMER_RIGHT_3_PENALTY=0.162074
PRIMER_LEFT_3_SEQUENCE=GGCATGAGAAGCAGGAGTTC
PRIMER_RIGHT_3_SEQUENCE=CCAAATCCAAATCCACAAGG
PRIMER_LEFT_3=305,20
PRIMER_RIGHT_3=464,20
PRIMER_LEFT_3_TM=59.957
PRIMER_RIGHT_3_TM=60.162
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=160
PRIMER_PAIR_3_PRODUCT_TM=71.0
PRIMER_PAIR_4_PENALTY=0.241297
PRIMER_LEFT_4_PENALTY=0.008461
PRIMER_RIGHT_4_PENALTY=0.232835
PRIMER_LEFT_4_SEQUENCE=GCTGACTTTGTCCATCTGCA
PRIMER_RIGHT_4_SEQUENCE=GGTGGAAACTCCAAATCCAA
PRIMER_LEFT_4=337,20
PRIMER_RIGHT_4=474,20
PRIMER_LEFT_4_TM=59.992
PRIMER_RIGHT_4_TM=59.767
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=138
PRIMER_PAIR_4_PRODUCT_TM=68.6
=
SEQUENCE_ID=23.RHAP131FH2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTCACTTCTGCTACACAGTTAGCTTCCTTTTCAAAACCTCGTCTTCCTACTTTGGATCCCTTTCTCACCTCCTCTTCTGCTTCCCTTTTTGTTATGGTGGATTGTTCTTGGCTGTCTGTCTGCTTTATTCTTCTTGATCCCCACACCCCTTATGAGTAGCCATGAGATCTCCAGATGTCCGAGGCTTCTGTTGCTCTTTGGAGTTGACTGACACATGCCTGAACCACAGGGTTCACTGATAGCTCTCTGTGGTCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCATGTATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=23,322
SEQUENCE_TARGET=281,46
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=23.RHAP131FH8.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCAGCATTTCCCAAGCTGGAGGAGCTANACAGACAGTAAGTAGTTTTTGGCTAATTAATTATCTTCCATAGAAAACAATGTTTCTCACAATTCGTCCTCTTTGTTAATATATTTTCAGAATGCCCCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCAGCCAGCATGCACTGCGTTTCTGAAATGTCTACTAATAGTAAAGTATGGCTATGATAAGTGACTGGTGACTGATGAGAGAGGTTTCCAGAGCTTCTGGACAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=15,285
SEQUENCE_TARGET=144,46
P3_COMMENT=CL is ATATTTTCAGAATGCCCCCC
P3_COMMENT=CR is TCCAGAAGCTCTGGAAACCT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.001046
PRIMER_LEFT_0_PENALTY=0.015502
PRIMER_RIGHT_0_PENALTY=0.985544
PRIMER_LEFT_0_SEQUENCE=ATATTTTCAGAATGCCCCCC
PRIMER_RIGHT_0_SEQUENCE=CCAGAAGCTCTGGAAACCTC
PRIMER_LEFT_0=122,20
PRIMER_RIGHT_0=293,20
PRIMER_LEFT_0_TM=59.984
PRIMER_RIGHT_0_TM=59.014
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=172
PRIMER_PAIR_0_PRODUCT_TM=69.3
PRIMER_PAIR_1_PENALTY=1.001046
PRIMER_LEFT_1_PENALTY=0.015502
PRIMER_RIGHT_1_PENALTY=0.985544
PRIMER_LEFT_1_SEQUENCE=ATATTTTCAGAATGCCCCCC
PRIMER_RIGHT_1_SEQUENCE=TCCAGAAGCTCTGGAAACCT
PRIMER_LEFT_1=122,20
PRIMER_RIGHT_1=294,20
PRIMER_LEFT_1_TM=59.984
PRIMER_RIGHT_1_TM=59.014
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=173
PRIMER_PAIR_1_PRODUCT_TM=69.2
PRIMER_PAIR_2_PENALTY=1.017442
PRIMER_LEFT_2_PENALTY=0.015502
PRIMER_RIGHT_2_PENALTY=1.001941
PRIMER_LEFT_2_SEQUENCE=ATATTTTCAGAATGCCCCCC
PRIMER_RIGHT_2_SEQUENCE=CCAGAAGCTCTGGAAACCTCT
PRIMER_LEFT_2=122,20
PRIMER_RIGHT_2=293,21
PRIMER_LEFT_2_TM=59.984
PRIMER_RIGHT_2_TM=60.002
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=172
PRIMER_PAIR_2_PRODUCT_TM=69.3
PRIMER_PAIR_3_PENALTY=1.145086
PRIMER_LEFT_3_PENALTY=0.015502
PRIMER_RIGHT_3_PENALTY=1.129584
PRIMER_LEFT_3_SEQUENCE=ATATTTTCAGAATGCCCCCC
PRIMER_RIGHT_3_SEQUENCE=GTCCAGAAGCTCTGGAAACCT
PRIMER_LEFT_3=122,20
PRIMER_RIGHT_3=295,21
PRIMER_LEFT_3_TM=59.984
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=174
PRIMER_PAIR_3_PRODUCT_TM=69.4
PRIMER_PAIR_4_PENALTY=1.147207
PRIMER_LEFT_4_PENALTY=0.015502
PRIMER_RIGHT_4_PENALTY=1.131705
PRIMER_LEFT_4_SEQUENCE=ATATTTTCAGAATGCCCCCC
PRIMER_RIGHT_4_SEQUENCE=GTCCAGAAGCTCTGGAAACC
PRIMER_LEFT_4=122,20
PRIMER_RIGHT_4=295,20
PRIMER_LEFT_4_TM=59.984
PRIMER_RIGHT_4_TM=58.868
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=69.4
=
SEQUENCE_ID=24.RHAP131FH9.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTCATTCTCCATCAAATGCCACGAACTTCAATGGCTAACCCTTGGGTTCCGTGTCTATGAGGAAGTATACACAATGCCAATGAGCCAGAGATATTCTGTAACCTTCTATTTCACAGTTCCTGTGATCAGCTCCTTAAAGTGGCAGAGGACATAGGAGTTGGCTTTCTCTAGTCATGTACAGTCTTATAAAAATCATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCAAGAGGAAAAATAGATTTAGTAAAAGCAAAAAAAATTGGTAATGGAGAAACAAAAATAATATTGAATCAAGACCTGAGCATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=15,337
SEQUENCE_TARGET=214,50
P3_COMMENT=CL is GCTCCTTAAAGTGGCAGAGG
P3_COMMENT=CR is TCTCCATTACCAATTTTTTTTGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.515792
PRIMER_LEFT_0_PENALTY=0.923358
PRIMER_RIGHT_0_PENALTY=3.592434
PRIMER_LEFT_0_SEQUENCE=GCTCCTTAAAGTGGCAGAGG
PRIMER_RIGHT_0_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
PRIMER_LEFT_0=143,20
PRIMER_RIGHT_0=315,23
PRIMER_LEFT_0_TM=59.077
PRIMER_RIGHT_0_TM=59.408
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=30.435
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=173
PRIMER_PAIR_0_PRODUCT_TM=66.4
PRIMER_PAIR_1_PENALTY=4.618441
PRIMER_LEFT_1_PENALTY=1.026007
PRIMER_RIGHT_1_PENALTY=3.592434
PRIMER_LEFT_1_SEQUENCE=AGCTCCTTAAAGTGGCAGAGG
PRIMER_RIGHT_1_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
PRIMER_LEFT_1=142,21
PRIMER_RIGHT_1=315,23
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.408
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=30.435
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=174
PRIMER_PAIR_1_PRODUCT_TM=66.4
PRIMER_PAIR_2_PENALTY=4.731626
PRIMER_LEFT_2_PENALTY=1.139192
PRIMER_RIGHT_2_PENALTY=3.592434
PRIMER_LEFT_2_SEQUENCE=TCCTTAAAGTGGCAGAGGACA
PRIMER_RIGHT_2_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
PRIMER_LEFT_2=145,21
PRIMER_RIGHT_2=315,23
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=59.408
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=171
PRIMER_PAIR_2_PRODUCT_TM=66.0
PRIMER_PAIR_3_PENALTY=4.854048
PRIMER_LEFT_3_PENALTY=1.261614
PRIMER_RIGHT_3_PENALTY=3.592434
PRIMER_LEFT_3_SEQUENCE=TGGCAGAGGACATAGGAGTTG
PRIMER_RIGHT_3_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
PRIMER_LEFT_3=154,21
PRIMER_RIGHT_3=315,23
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=59.408
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=30.435
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=162
PRIMER_PAIR_3_PRODUCT_TM=65.6
PRIMER_PAIR_4_PENALTY=4.934726
PRIMER_LEFT_4_PENALTY=0.923358
PRIMER_RIGHT_4_PENALTY=4.011367
PRIMER_LEFT_4_SEQUENCE=GCTCCTTAAAGTGGCAGAGG
PRIMER_RIGHT_4_SEQUENCE=TCCATTACCAATTTTTTTTGCTT
PRIMER_LEFT_4=143,20
PRIMER_RIGHT_4=313,23
PRIMER_LEFT_4_TM=59.077
PRIMER_RIGHT_4_TM=58.989
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=26.087
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=171
PRIMER_PAIR_4_PRODUCT_TM=66.2
=
SEQUENCE_ID=25.RHAP131FA10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAGGAAGATATTTTATACATAAAATATTGAATATGTTTTACCACAATGGGGCATAGCACAATGTAAAGCATTTCACTATCATAATAGAGAGTTTTTGATTCTAGAAATGTGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAGCCCACAGGGAGAGNNNNNNNNNNNNNNAGAGAGAAATACAAAATGTAATTATCTTTAATTCTAAGAACCCTGCTATGATGGTTAGTTTTTATTGCCAACTTGGATGGGTTTAGAACCAGCCTGGCAACAAAACTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=27,304
SEQUENCE_TARGET=143,42
SEQUENCE_TARGET=206,14
P3_COMMENT=CL is TGGGGCATAGCACAATGTAA
P3_COMMENT=CR is CTCTCCCTGTGGGCTCTGT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.451089
PRIMER_LEFT_0_PENALTY=0.046801
PRIMER_RIGHT_0_PENALTY=1.404288
PRIMER_LEFT_0_SEQUENCE=TGGGGCATAGCACAATGTAA
PRIMER_RIGHT_0_SEQUENCE=CTCTCCCTGTGGGCTCTGT
PRIMER_LEFT_0=75,20
PRIMER_RIGHT_0=205,19
PRIMER_LEFT_0_TM=59.953
PRIMER_RIGHT_0_TM=60.404
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=131
PRIMER_PAIR_0_PRODUCT_TM=67.2
PRIMER_PAIR_1_PENALTY=1.471724
PRIMER_LEFT_1_PENALTY=1.404288
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=ACAGAGCCCACAGGGAGAG
PRIMER_RIGHT_1_SEQUENCE=GCTGGTTCTAAACCCATCCA
PRIMER_LEFT_1=187,19
PRIMER_RIGHT_1=312,20
PRIMER_LEFT_1_TM=60.404
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=63.158
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=126
PRIMER_PAIR_1_PRODUCT_TM=69.1
PRIMER_PAIR_2_PENALTY=1.625656
PRIMER_LEFT_2_PENALTY=0.221368
PRIMER_RIGHT_2_PENALTY=1.404288
PRIMER_LEFT_2_SEQUENCE=AATGGGGCATAGCACAATGT
PRIMER_RIGHT_2_SEQUENCE=CTCTCCCTGTGGGCTCTGT
PRIMER_LEFT_2=73,20
PRIMER_RIGHT_2=205,19
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=60.404
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=63.158
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=133
PRIMER_PAIR_2_PRODUCT_TM=67.1
PRIMER_PAIR_3_PENALTY=1.625656
PRIMER_LEFT_3_PENALTY=0.221368
PRIMER_RIGHT_3_PENALTY=1.404288
PRIMER_LEFT_3_SEQUENCE=ACAATGGGGCATAGCACAAT
PRIMER_RIGHT_3_SEQUENCE=CTCTCCCTGTGGGCTCTGT
PRIMER_LEFT_3=71,20
PRIMER_RIGHT_3=205,19
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=60.404
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=135
PRIMER_PAIR_3_PRODUCT_TM=67.3
PRIMER_PAIR_4_PENALTY=1.653189
PRIMER_LEFT_4_PENALTY=1.404288
PRIMER_RIGHT_4_PENALTY=0.248900
PRIMER_LEFT_4_SEQUENCE=ACAGAGCCCACAGGGAGAG
PRIMER_RIGHT_4_SEQUENCE=TTGCCAGGCTGGTTCTAAAC
PRIMER_LEFT_4=187,19
PRIMER_RIGHT_4=319,20
PRIMER_LEFT_4_TM=60.404
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=133
PRIMER_PAIR_4_PRODUCT_TM=69.9
=
SEQUENCE_ID=26.RHAP131FA11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCCTACTACAACAAATCAATCTAAAAATAGACCTTTTATTAGCTCACAATTTTAAGGATTTTCTTTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=15,130
SEQUENCE_TARGET=15,57
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=26.RHAP131FA5.seq
SEQUENCE_TEMPLATE=CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCCTCATTGTTGTTTTGATTTCAGGTTTATGCTAACTGAATTTTGTGTTACCTTGACTGGGTCATGAGACCATGCTATTCCTTTGAACCTTTTTCTGACTTTTGTGAGGACACTTTCTGTTGAGCTCAATATAGACTGATGAAAGTAGATTTTCCTCCCTGACAGAAGCCCATCTAACCAGCCTGAGCAAAATGATTAGTCTCCTTTCAGGTGGAAGCCAACTCCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=0,277
SEQUENCE_TARGET=3,42
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=27.RHAP131FA12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCGACAAAGATGACATGGTTTCTANTGGAGGCAGGGCTGAGATGAGGAGTCACATTCTCTATTGGCAGGCATTTCCCCCTGTCTTGTTATTTTCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNNNNNNNTGTNNCNNNNNGNGNNNNNCNCNACACAACGNNTGTGCGTGAAAANTGAAGTTTTTTGTGTGTCAGTGAGACAGTGTCTTCNTGGGTGCCCGAGGCAANCNAGGGNATCCACCCGAGCGTTAGCCTCCAGANATTATTAACAANGGCCAAAACATCATNCCTACCTAAGTGAGAAAAAGCAATTTNGANAAAAANCGNGCTCCNAATCCAAAACNTGGGCATGGGCTAGATCCNNAGGTGAAAATNGTAGGCGGTCNAGATTCCNAACAACNAANNANCCGGAAACATGNTGTGTGATAACCANAGGGTGACNNNNGAGTNANNNACACAAAATAANTGCGGNGCGCACAAAGNGCGNNNTCCAGNGGGGAAACCTGNGNGNGCCAACTTNNATAAANNNGNNGGGAANNANCGGGNGNGTTGCGAGGTTGNGNAANNNNNCCNCGGNAGNNTNGTANNNNCNCGNNNANGNGGGGGGAGNGNGNTTGCNNNNNGGGNNNCCTGNNNANNNTTNNNANAGTGNAANGGGGANNNCCAANNNNGNAA
SEQUENCE_INCLUDED_REGION=14,687
SEQUENCE_TARGET=141,14
SEQUENCE_TARGET=111,28
P3_COMMENT=CL is CTCTATTGGCAGGCATTTCC
P3_COMMENT=CR is AGACACTGTCTCACTGACACACAA
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.420604
PRIMER_LEFT_0_PENALTY=0.332028
PRIMER_RIGHT_0_PENALTY=4.088575
PRIMER_LEFT_0_SEQUENCE=CTCTATTGGCAGGCATTTCC
PRIMER_RIGHT_0_SEQUENCE=AGACACTGTCTCACTGACACACAA
PRIMER_LEFT_0=70,20
PRIMER_RIGHT_0=233,24
PRIMER_LEFT_0_TM=59.668
PRIMER_RIGHT_0_TM=59.911
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.833
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=164
PRIMER_PAIR_0_PRODUCT_TM=68.0
PRIMER_PAIR_1_PENALTY=4.420604
PRIMER_LEFT_1_PENALTY=0.332028
PRIMER_RIGHT_1_PENALTY=4.088575
PRIMER_LEFT_1_SEQUENCE=CTCTATTGGCAGGCATTTCC
PRIMER_RIGHT_1_SEQUENCE=AAGACACTGTCTCACTGACACACA
PRIMER_LEFT_1=70,20
PRIMER_RIGHT_1=234,24
PRIMER_LEFT_1_TM=59.668
PRIMER_RIGHT_1_TM=59.911
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=9.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=165
PRIMER_PAIR_1_PRODUCT_TM=67.9
PRIMER_PAIR_2_PENALTY=4.438083
PRIMER_LEFT_2_PENALTY=0.332028
PRIMER_RIGHT_2_PENALTY=4.106055
PRIMER_LEFT_2_SEQUENCE=CTCTATTGGCAGGCATTTCC
PRIMER_RIGHT_2_SEQUENCE=CACTGTCTCACTGACACACAAAA
PRIMER_LEFT_2=70,20
PRIMER_RIGHT_2=230,23
PRIMER_LEFT_2_TM=59.668
PRIMER_RIGHT_2_TM=58.894
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=9.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=161
PRIMER_PAIR_2_PRODUCT_TM=67.9
PRIMER_PAIR_3_PENALTY=4.481654
PRIMER_LEFT_3_PENALTY=0.332028
PRIMER_RIGHT_3_PENALTY=4.149625
PRIMER_LEFT_3_SEQUENCE=CTCTATTGGCAGGCATTTCC
PRIMER_RIGHT_3_SEQUENCE=GACACTGTCTCACTGACACACAA
PRIMER_LEFT_3=70,20
PRIMER_RIGHT_3=232,23
PRIMER_LEFT_3_TM=59.668
PRIMER_RIGHT_3_TM=58.850
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=163
PRIMER_PAIR_3_PRODUCT_TM=68.0
PRIMER_PAIR_4_PENALTY=4.556370
PRIMER_LEFT_4_PENALTY=0.332028
PRIMER_RIGHT_4_PENALTY=4.224341
PRIMER_LEFT_4_SEQUENCE=CTCTATTGGCAGGCATTTCC
PRIMER_RIGHT_4_SEQUENCE=ACACTGTCTCACTGACACACAAAA
PRIMER_LEFT_4=70,20
PRIMER_RIGHT_4=231,24
PRIMER_LEFT_4_TM=59.668
PRIMER_RIGHT_4_TM=59.776
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=9.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=162
PRIMER_PAIR_4_PRODUCT_TM=67.8
=
SEQUENCE_ID=29.RHAP131FB5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTTGGTGCATGCAGCCACATTTCCTCAAGGAGCATAGGGCTTATACAGTTCTTTCAGCACAAGTTTTGGGTCTTTTGTTCCACATTCACATGTCAGACTTGCTAGCCGGTTTTTTGTATATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATATGTATTTGTGTTATATGTGTGTGTGTGTTTGCAAGTGCGTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTGTATTCGTGTTATTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTGTGTGTGTGCTATTTCTATNNGTTTTTATNTGTANNNNNNNNNNNNNNNNATNTGCGTGTGTGTGTGCNNNNNNNNNNNNNNNNNNNNNGTGTNNTCCNCNCCNGTATTTCACACNNAANACGTNNANNGNGAGAATTCCTNAAAATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=23,477
SEQUENCE_TARGET=148,44
SEQUENCE_TARGET=243,35
SEQUENCE_TARGET=301,40
SEQUENCE_TARGET=382,16
SEQUENCE_TARGET=416,18
P3_COMMENT=CL is CATGTCAGACTTGCTAGCCG
P3_COMMENT=CR is ACGCACTTGCAAACACACAC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.218921
PRIMER_LEFT_0_PENALTY=0.384829
PRIMER_RIGHT_0_PENALTY=0.834091
PRIMER_LEFT_0_SEQUENCE=CATGTCAGACTTGCTAGCCG
PRIMER_RIGHT_0_SEQUENCE=ACGCACTTGCAAACACACAC
PRIMER_LEFT_0=112,20
PRIMER_RIGHT_0=236,20
PRIMER_LEFT_0_TM=59.615
PRIMER_RIGHT_0_TM=60.834
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=11.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=125
PRIMER_PAIR_0_PRODUCT_TM=64.9
PRIMER_PAIR_1_PENALTY=1.456124
PRIMER_LEFT_1_PENALTY=0.384829
PRIMER_RIGHT_1_PENALTY=1.071294
PRIMER_LEFT_1_SEQUENCE=CATGTCAGACTTGCTAGCCG
PRIMER_RIGHT_1_SEQUENCE=ACGCACTTGCAAACACACA
PRIMER_LEFT_1=112,20
PRIMER_RIGHT_1=236,19
PRIMER_LEFT_1_TM=59.615
PRIMER_RIGHT_1_TM=59.929
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=47.368
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_1_PRODUCT_TM=64.9
PRIMER_PAIR_2_PENALTY=1.456124
PRIMER_LEFT_2_PENALTY=0.384829
PRIMER_RIGHT_2_PENALTY=1.071294
PRIMER_LEFT_2_SEQUENCE=CATGTCAGACTTGCTAGCCG
PRIMER_RIGHT_2_SEQUENCE=CACGCACTTGCAAACACAC
PRIMER_LEFT_2=112,20
PRIMER_RIGHT_2=237,19
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=59.929
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=126
PRIMER_PAIR_2_PRODUCT_TM=65.2
PRIMER_PAIR_3_PENALTY=1.489980
PRIMER_LEFT_3_PENALTY=0.418685
PRIMER_RIGHT_3_PENALTY=1.071294
PRIMER_LEFT_3_SEQUENCE=ATGTCAGACTTGCTAGCCGG
PRIMER_RIGHT_3_SEQUENCE=CACGCACTTGCAAACACAC
PRIMER_LEFT_3=113,20
PRIMER_RIGHT_3=237,19
PRIMER_LEFT_3_TM=60.419
PRIMER_RIGHT_3_TM=59.929
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=125
PRIMER_PAIR_3_PRODUCT_TM=64.9
PRIMER_PAIR_4_PENALTY=1.838763
PRIMER_LEFT_4_PENALTY=1.004672
PRIMER_RIGHT_4_PENALTY=0.834091
PRIMER_LEFT_4_SEQUENCE=TTTGGGTCTTTTGTTCCACA
PRIMER_RIGHT_4_SEQUENCE=ACGCACTTGCAAACACACAC
PRIMER_LEFT_4=88,20
PRIMER_RIGHT_4=236,20
PRIMER_LEFT_4_TM=58.995
PRIMER_RIGHT_4_TM=60.834
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=149
PRIMER_PAIR_4_PRODUCT_TM=66.6
=
SEQUENCE_ID=30.RHAP131FB12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCGGACAGCTTTCTCCCTAGCTTTTCATTTCTGACAATAGTGCTCTGCCTTGATTCTAGAATGATTTCATGGAGGGAAGTTTGTCCTTTTTAGAGCTATCACAGCTTCACAGTCCTACCCACTTCACTTGTTTCTATGCCAATTCCCTAATACTTGGAGCAGTAATCACACAGCAATACCACAAGGGTGCTTACCCACAGTGCTTTCCTCAGTCAGGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGGTGCTTACCCACAGTGCTTTCCTTAGTCAGGAGCGTGTGTGTGTGTGGGGGGGGGGGGAGGCCAAGATGATAGCCCCTGGGATGGCACAGGTTACTTCCTTGGAGGACCTAACACCAAGACCAATGTGACCATTGCAGCCCACATCTGCACCATCTTTAGAACAACATCCTTTAGGGATACCCTCAAGAAAACACCAGTGATCAGTCTGTGATAGGCAGAGGACAGGTAGGGCNCNTCAAGCCCTTGATCCNCGTGTCNNTGACCACGCCTGGTGATGGGGATCTCNCGTCTTGTTTACCCCATGAGATGGCAGACCCACATGGGGGACGAGCACGNATCNGAAACATGGCAGAGNTGTTCCTGTGTAAANTGGTANCGGCACACTC
SEQUENCE_INCLUDED_REGION=14,666
SEQUENCE_TARGET=234,54
P3_COMMENT=CL is GCAATACCACAAGGGTGCTT
P3_COMMENT=CR is GGAAAGCACTGTGGGTAAGC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.259740
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.259704
PRIMER_LEFT_0_SEQUENCE=GCAATACCACAAGGGTGCTT
PRIMER_RIGHT_0_SEQUENCE=GGAAAGCACTGTGGGTAAGC
PRIMER_LEFT_0=186,20
PRIMER_RIGHT_0=314,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.740
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=129
PRIMER_PAIR_0_PRODUCT_TM=68.3
PRIMER_PAIR_1_PENALTY=0.509682
PRIMER_LEFT_1_PENALTY=0.259704
PRIMER_RIGHT_1_PENALTY=0.249979
PRIMER_LEFT_1_SEQUENCE=GCTTACCCACAGTGCTTTCC
PRIMER_RIGHT_1_SEQUENCE=ATCCCAGGGGCTATCATCTT
PRIMER_LEFT_1=202,20
PRIMER_RIGHT_1=375,20
PRIMER_LEFT_1_TM=59.740
PRIMER_RIGHT_1_TM=59.750
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=174
PRIMER_PAIR_1_PRODUCT_TM=73.2
PRIMER_PAIR_2_PENALTY=0.547997
PRIMER_LEFT_2_PENALTY=0.298018
PRIMER_RIGHT_2_PENALTY=0.249979
PRIMER_LEFT_2_SEQUENCE=CCCACAGTGCTTTCCTCAGT
PRIMER_RIGHT_2_SEQUENCE=ATCCCAGGGGCTATCATCTT
PRIMER_LEFT_2=207,20
PRIMER_RIGHT_2=375,20
PRIMER_LEFT_2_TM=60.298
PRIMER_RIGHT_2_TM=59.750
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=169
PRIMER_PAIR_2_PRODUCT_TM=73.1
PRIMER_PAIR_3_PENALTY=0.547997
PRIMER_LEFT_3_PENALTY=0.298018
PRIMER_RIGHT_3_PENALTY=0.249979
PRIMER_LEFT_3_SEQUENCE=ACCCACAGTGCTTTCCTCAG
PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGGCTATCATCTT
PRIMER_LEFT_3=206,20
PRIMER_RIGHT_3=375,20
PRIMER_LEFT_3_TM=60.298
PRIMER_RIGHT_3_TM=59.750
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=170
PRIMER_PAIR_3_PRODUCT_TM=73.0
PRIMER_PAIR_4_PENALTY=0.557677
PRIMER_LEFT_4_PENALTY=0.378958
PRIMER_RIGHT_4_PENALTY=0.178718
PRIMER_LEFT_4_SEQUENCE=AGCAATACCACAAGGGTGCT
PRIMER_RIGHT_4_SEQUENCE=AAGCACTGTGGGTAAGCACC
PRIMER_LEFT_4=185,20
PRIMER_RIGHT_4=311,20
PRIMER_LEFT_4_TM=59.621
PRIMER_RIGHT_4_TM=60.179
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PAIR_4_PRODUCT_SIZE=127
PRIMER_PAIR_4_PRODUCT_TM=67.8
=
SEQUENCE_ID=31.RHAP131FC4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCAAATGTGAGCAGATACTCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATGTCATGATAAGGACCACTTTTGTACACTAACAAAAAACTGGATTTAAGCAATATATTTTAAAAACCTCAGCTATATAATAACAAAATAAAAGGGATAATAGAGCTATATGACATCTTACACACATATGCACACCATAGATGTTGTGTAGAGAAAGATGACACTGAGTTCCTGACCCCCAGCTCCTCCCACTGAGTACTATAATCACAGGTGTGTACCAGCAGTCCAGTTTCATTGCNTTAAGAATTAAAGCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=24,327
SEQUENCE_TARGET=48,42
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=32.RHAP131FC5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNCCCTGTGTTTCATTCTGAGTGCTACAGAGAAGCATTTTTGACAAGTTTAAGCTCTTACTTAAACTTGAGACCAATGATGAGTGAAAGGCATATCCTATCAAATTGCATACATGATATGCAAAATTAAGTCTACATTTTAACATGCCTCGCCTATTGTGGACATATTCTTTTATGAATTGTGTTTAACTACTTACTAAAGGTAGCTCTTTTCCTTTGTGTGTGCATGCACNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGNGACNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAACAGNTTTTNAAAAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=11,311
SEQUENCE_TARGET=240,26
SEQUENCE_TARGET=274,24
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=33.RHAP131FC12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNTTGTATTAGAACACTGAATAACTCTTATCTTTACTCTGTAATTTAAAAATTACATTCTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCATATATGGAAGTCAGAGGACAATTTGTGGGAAGTTCTCTCTCTATACTGTGTGGGTCCTGAGGAATGAATTTATGTCACCAGGCTTGGTGACCAGCACCTTGACTGGATAATCCACCTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=13,232
SEQUENCE_TARGET=75,44
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=33.RHAP131FC6.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNCCGCCTCCAAGGACTCCAAGACTGGATGCACACGCTGAGGCAAGACTGCTGAGGCGACACATGGAGGACACATGATGGTTAGAGGGTATATAATAAGGACTCCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCACTCGGCTTCCTGGTACACTAGTTGTGTCACACTTGCTGGGCTTTGCTGACCTTTGCTTCACTCAGAGAGGCACAGCTGAAGAACGTCTGATGTTCCTGCTGGTCCCTCCTGCTGACTGGAGCCCGGGCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=10,330
SEQUENCE_TARGET=118,44
SEQUENCE_TARGET=163,40
P3_COMMENT=CL is ACATGGAGGACACATGATGG
P3_COMMENT=CR is GTGTACCAGGAAGCCGAGTG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.526938
PRIMER_LEFT_0_PENALTY=0.815754
PRIMER_RIGHT_0_PENALTY=0.711184
PRIMER_LEFT_0_SEQUENCE=CATGGAGGACACATGATGGT
PRIMER_RIGHT_0_SEQUENCE=GTGTACCAGGAAGCCGAGTG
PRIMER_LEFT_0=69,20
PRIMER_RIGHT_0=224,20
PRIMER_LEFT_0_TM=59.184
PRIMER_RIGHT_0_TM=60.711
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.8000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=156
PRIMER_PAIR_0_PRODUCT_TM=65.5
PRIMER_PAIR_1_PENALTY=1.526938
PRIMER_LEFT_1_PENALTY=0.815754
PRIMER_RIGHT_1_PENALTY=0.711184
PRIMER_LEFT_1_SEQUENCE=ACATGGAGGACACATGATGG
PRIMER_RIGHT_1_SEQUENCE=GTGTACCAGGAAGCCGAGTG
PRIMER_LEFT_1=68,20
PRIMER_RIGHT_1=224,20
PRIMER_LEFT_1_TM=59.184
PRIMER_RIGHT_1_TM=60.711
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=157
PRIMER_PAIR_1_PRODUCT_TM=65.5
PRIMER_PAIR_2_PENALTY=1.715371
PRIMER_LEFT_2_PENALTY=1.004187
PRIMER_RIGHT_2_PENALTY=0.711184
PRIMER_LEFT_2_SEQUENCE=GACTGCTGAGGCGACACAT
PRIMER_RIGHT_2_SEQUENCE=GTGTACCAGGAAGCCGAGTG
PRIMER_LEFT_2=53,19
PRIMER_RIGHT_2=224,20
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.711
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=172
PRIMER_PAIR_2_PRODUCT_TM=67.4
PRIMER_PAIR_3_PENALTY=1.749238
PRIMER_LEFT_3_PENALTY=1.038054
PRIMER_RIGHT_3_PENALTY=0.711184
PRIMER_LEFT_3_SEQUENCE=CGACACATGGAGGACACATG
PRIMER_RIGHT_3_SEQUENCE=GTGTACCAGGAAGCCGAGTG
PRIMER_LEFT_3=64,20
PRIMER_RIGHT_3=224,20
PRIMER_LEFT_3_TM=61.038
PRIMER_RIGHT_3_TM=60.711
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=161
PRIMER_PAIR_3_PRODUCT_TM=66.1
PRIMER_PAIR_4_PENALTY=1.753105
PRIMER_LEFT_4_PENALTY=1.041921
PRIMER_RIGHT_4_PENALTY=0.711184
PRIMER_LEFT_4_SEQUENCE=AGACTGCTGAGGCGACACAT
PRIMER_RIGHT_4_SEQUENCE=GTGTACCAGGAAGCCGAGTG
PRIMER_LEFT_4=52,20
PRIMER_RIGHT_4=224,20
PRIMER_LEFT_4_TM=61.042
PRIMER_RIGHT_4_TM=60.711
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=173
PRIMER_PAIR_4_PRODUCT_TM=67.3
=
SEQUENCE_ID=35.RHAP131FD11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCAGTGGTTAGCAAGCAACACTGAAGAGACTTGTCAGGTTATATTTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCAACAATGATTAAAGAAGAGGAGGCTGTGAAATTTAAGAGCGAGCAGGGGTGGGTATTGAAGAGATTAGAGGGGGAATGTAATTATGTCTTCATTAACTTTTTCAAATTAAAAAAAAAGAATGTGACTTTAATCATATCTAAATCGGAGCAGACTATCAATCCACATACTCCACTCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=25,317
SEQUENCE_TARGET=77,83
P3_COMMENT=CL is CAACACTGAAGAGACTTGTCAGG
P3_COMMENT=CR is ACCCCTGCTCGCTCTTAAAT
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.180272
PRIMER_LEFT_0_PENALTY=3.951808
PRIMER_RIGHT_0_PENALTY=0.228464
PRIMER_LEFT_0_SEQUENCE=CAACACTGAAGAGACTTGTCAGG
PRIMER_RIGHT_0_SEQUENCE=ACCCCTGCTCGCTCTTAAAT
PRIMER_LEFT_0=39,23
PRIMER_RIGHT_0=213,20
PRIMER_LEFT_0_TM=59.048
PRIMER_RIGHT_0_TM=60.228
PRIMER_LEFT_0_GC_PERCENT=47.826
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=175
PRIMER_PAIR_0_PRODUCT_TM=64.7
PRIMER_PAIR_1_PENALTY=4.291559
PRIMER_LEFT_1_PENALTY=1.478935
PRIMER_RIGHT_1_PENALTY=2.812624
PRIMER_LEFT_1_SEQUENCE=TGGTTAGCAAGCAACACTGAA
PRIMER_RIGHT_1_SEQUENCE=TTTCACAGCCTCCTCTTCTTT
PRIMER_LEFT_1=28,21
PRIMER_RIGHT_1=194,21
PRIMER_LEFT_1_TM=59.521
PRIMER_RIGHT_1_TM=58.187
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=167
PRIMER_PAIR_1_PRODUCT_TM=63.7
PRIMER_PAIR_2_PENALTY=4.557741
PRIMER_LEFT_2_PENALTY=3.855493
PRIMER_RIGHT_2_PENALTY=0.702248
PRIMER_LEFT_2_SEQUENCE=GCAACACTGAAGAGACTTGTCA
PRIMER_RIGHT_2_SEQUENCE=CCCCTGCTCGCTCTTAAATT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=212,20
PRIMER_LEFT_2_TM=58.145
PRIMER_RIGHT_2_TM=60.702
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=175
PRIMER_PAIR_2_PRODUCT_TM=64.9
PRIMER_PAIR_3_PENALTY=4.579361
PRIMER_LEFT_3_PENALTY=1.478935
PRIMER_RIGHT_3_PENALTY=3.100425
PRIMER_LEFT_3_SEQUENCE=TGGTTAGCAAGCAACACTGAA
PRIMER_RIGHT_3_SEQUENCE=CACAGCCTCCTCTTCTTTAATCA
PRIMER_LEFT_3=28,21
PRIMER_RIGHT_3=191,23
PRIMER_LEFT_3_TM=59.521
PRIMER_RIGHT_3_TM=59.900
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=164
PRIMER_PAIR_3_PRODUCT_TM=63.7
PRIMER_PAIR_4_PENALTY=4.654056
PRIMER_LEFT_4_PENALTY=3.951808
PRIMER_RIGHT_4_PENALTY=0.702248
PRIMER_LEFT_4_SEQUENCE=CAACACTGAAGAGACTTGTCAGG
PRIMER_RIGHT_4_SEQUENCE=CCCCTGCTCGCTCTTAAATT
PRIMER_LEFT_4=39,23
PRIMER_RIGHT_4=212,20
PRIMER_LEFT_4_TM=59.048
PRIMER_RIGHT_4_TM=60.702
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=174
PRIMER_PAIR_4_PRODUCT_TM=64.7
=
SEQUENCE_ID=35.RHAP131FD5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNGNTTAAAATTTCAGTTGTCTTCACTTCACATCCAAGGCTGGATTTCTTGGGATGTGATACAGTGCGGATGGTGCTTGTGGGATCACACAATCTTATGAGACCTGTCTTTCTGTGTTGCCTGTAAACTGTTTCCTAAATANTTTGAGTAGATCTGTTACCTTCTAAATAAGGTTAGATCACTCAAATAACGTATTTGTCTCTAGCTTCTATCTCAGTCACATTTTAATTATTTATACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTCCATTAACTTTGCCTTNTGANGANGACTCCTTTNTCTTTCANTCTNTTGGGNGGTANCGAAGCTCGNANTCGNNAATNGNNGTCNNNGNTGTTTTCCNGTGTNANNTTNNTNNCCGCNCACNATTCCANNCNNAATANGNNGCGGNAGGATANAAGTGGTANANCNGGGGGNNNNNAAANGNGTTGGGNNANTCAGNNTTANNNGGGGGGNNNNTNNCGNTTNNCAANNNGGGNAANNNNNNNTGNANNNGNNTTAATGNNTNGGGNNANG
SEQUENCE_INCLUDED_REGION=11,568
SEQUENCE_TARGET=250,50
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=38.RHAP131FE11.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCAATGAGCAACCCTGTCTTAAAACAACTGTGTGTGTTTTTCGGGGGGTCAAGGGGGCTGTTCCTGAGGAATGATTCAAGGTTGATCTCTGGCACCTGTGCTTGTGTGCACACATATGCACAAGTGTATGCACACATACACAACAGAATCTTCCAACACTTTGACCTTGGACTTTCCCTCTTTCAGAAGCAAGAGAAACAGATATCTGTTGTTTAAACCACTTAGTCTACAGTGATTGGTCACTTGGTGTCCTGTGCTATGAGGCGTGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTGAAATTAGAAACCACCCTTATCACCATGGTTTTCAGAGCCCAGTATCAGTTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=14,402
SEQUENCE_TARGET=286,70
P3_COMMENT=CL is CTGTGCTATGAGGCGTGTGT
P3_COMMENT=CR is ACTGGGCTCTGAAAACCATG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.182171
PRIMER_LEFT_0_PENALTY=0.071094
PRIMER_RIGHT_0_PENALTY=0.111077
PRIMER_LEFT_0_SEQUENCE=CTGTGCTATGAGGCGTGTGT
PRIMER_RIGHT_0_SEQUENCE=ACTGGGCTCTGAAAACCATG
PRIMER_LEFT_0=265,20
PRIMER_RIGHT_0=404,20
PRIMER_LEFT_0_TM=59.929
PRIMER_RIGHT_0_TM=60.111
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=140
PRIMER_PAIR_0_PRODUCT_TM=65.0
PRIMER_PAIR_1_PENALTY=0.410019
PRIMER_LEFT_1_PENALTY=0.298942
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=TGGTGTCCTGTGCTATGAGG
PRIMER_RIGHT_1_SEQUENCE=ACTGGGCTCTGAAAACCATG
PRIMER_LEFT_1=258,20
PRIMER_RIGHT_1=404,20
PRIMER_LEFT_1_TM=59.701
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=147
PRIMER_PAIR_1_PRODUCT_TM=65.9
PRIMER_PAIR_2_PENALTY=0.544789
PRIMER_LEFT_2_PENALTY=0.071094
PRIMER_RIGHT_2_PENALTY=0.473695
PRIMER_LEFT_2_SEQUENCE=CTGTGCTATGAGGCGTGTGT
PRIMER_RIGHT_2_SEQUENCE=CCATGGTGATAAGGGTGGTT
PRIMER_LEFT_2=265,20
PRIMER_RIGHT_2=389,20
PRIMER_LEFT_2_TM=59.929
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=125
PRIMER_PAIR_2_PRODUCT_TM=63.3
PRIMER_PAIR_3_PENALTY=0.544789
PRIMER_LEFT_3_PENALTY=0.071094
PRIMER_RIGHT_3_PENALTY=0.473695
PRIMER_LEFT_3_SEQUENCE=CTGTGCTATGAGGCGTGTGT
PRIMER_RIGHT_3_SEQUENCE=AACCATGGTGATAAGGGTGG
PRIMER_LEFT_3=265,20
PRIMER_RIGHT_3=391,20
PRIMER_LEFT_3_TM=59.929
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=127
PRIMER_PAIR_3_PRODUCT_TM=63.2
PRIMER_PAIR_4_PENALTY=0.737696
PRIMER_LEFT_4_PENALTY=0.626619
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=TGGTCACTTGGTGTCCTGTG
PRIMER_RIGHT_4_SEQUENCE=ACTGGGCTCTGAAAACCATG
PRIMER_LEFT_4=250,20
PRIMER_RIGHT_4=404,20
PRIMER_LEFT_4_TM=60.627
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=155
PRIMER_PAIR_4_PRODUCT_TM=66.6
=
SEQUENCE_ID=38.RHAP131FE5.seq
SEQUENCE_TEMPLATE=NNNNNNNNNCANCTCCGAAAAAAANNCCAAAACTAGTTCTCAGCTCTAGATAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCCTTACCTTGATCCTGACGCCCTTGTGTCATTTGTAGGTCAACCAACCACCCATACGTTTCAAGCAATAAAGAATCCAGAGAAAATAATCACNNNNNNNNNNNNNNNNNNGAGTTTTCTGACAGATGCCCAAAGATGCTTTCCAAAGCGTATGTTAGAAAGTGTGAACGAATGAATTCCTCTCTCCCTTCCCGCCCTAAAACACGAACTCACATTCTCCCAGAGATCCACTCCACTCTCCTGTTCCTTCTGTGATAGTTAATTTTGTCTCTTAGCATCACTAGATTTCCAGTCACCTAAGAAACATACCCCTGGGTGTGTGTGTCTTTTGGGGTGATCCTGCAAAAGGTTTAACTGAGGAGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=9,456
SEQUENCE_TARGET=59,32
SEQUENCE_TARGET=190,18
P3_COMMENT=CL is CTTACCTTGATCCTGACGCC
P3_COMMENT=CR is GCTTTGGAAAGCATCTTTGG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.483158
PRIMER_LEFT_0_PENALTY=0.306150
PRIMER_RIGHT_0_PENALTY=0.177008
PRIMER_LEFT_0_SEQUENCE=CTTACCTTGATCCTGACGCC
PRIMER_RIGHT_0_SEQUENCE=GCTTTGGAAAGCATCTTTGG
PRIMER_LEFT_0=99,20
PRIMER_RIGHT_0=245,20
PRIMER_LEFT_0_TM=59.694
PRIMER_RIGHT_0_TM=59.823
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=11.1000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=147
PRIMER_PAIR_0_PRODUCT_TM=71.2
PRIMER_PAIR_1_PENALTY=0.483158
PRIMER_LEFT_1_PENALTY=0.306150
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_LEFT_1_SEQUENCE=CCTTACCTTGATCCTGACGC
PRIMER_RIGHT_1_SEQUENCE=GCTTTGGAAAGCATCTTTGG
PRIMER_LEFT_1=98,20
PRIMER_RIGHT_1=245,20
PRIMER_LEFT_1_TM=59.694
PRIMER_RIGHT_1_TM=59.823
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=148
PRIMER_PAIR_1_PRODUCT_TM=71.4
PRIMER_PAIR_2_PENALTY=0.483158
PRIMER_LEFT_2_PENALTY=0.306150
PRIMER_RIGHT_2_PENALTY=0.177008
PRIMER_LEFT_2_SEQUENCE=GCCTTACCTTGATCCTGACG
PRIMER_RIGHT_2_SEQUENCE=GCTTTGGAAAGCATCTTTGG
PRIMER_LEFT_2=97,20
PRIMER_RIGHT_2=245,20
PRIMER_LEFT_2_TM=59.694
PRIMER_RIGHT_2_TM=59.823
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=149
PRIMER_PAIR_2_PRODUCT_TM=71.6
PRIMER_PAIR_3_PENALTY=0.489018
PRIMER_LEFT_3_PENALTY=0.312010
PRIMER_RIGHT_3_PENALTY=0.177008
PRIMER_LEFT_3_SEQUENCE=CCTGCCTTACCTTGATCCTG
PRIMER_RIGHT_3_SEQUENCE=GCTTTGGAAAGCATCTTTGG
PRIMER_LEFT_3=94,20
PRIMER_RIGHT_3=245,20
PRIMER_LEFT_3_TM=59.688
PRIMER_RIGHT_3_TM=59.823
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=152
PRIMER_PAIR_3_PRODUCT_TM=71.9
PRIMER_PAIR_4_PENALTY=0.519655
PRIMER_LEFT_4_PENALTY=0.306150
PRIMER_RIGHT_4_PENALTY=0.213506
PRIMER_LEFT_4_SEQUENCE=CTTACCTTGATCCTGACGCC
PRIMER_RIGHT_4_SEQUENCE=AAAGCATCTTTGGGCATCTG
PRIMER_LEFT_4=99,20
PRIMER_RIGHT_4=238,20
PRIMER_LEFT_4_TM=59.694
PRIMER_RIGHT_4_TM=60.214
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=140
PRIMER_PAIR_4_PRODUCT_TM=70.6
=
SEQUENCE_ID=40.RHAP131FF4.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAGTCACACAAAGATAGAGTATAGAATAGAGTTTATTTAGGGCATGGGGAGGAGAGTTGAAAGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGTAGAGGCTGGTGGTGAGCACGTAGAGAGAAGGGAAGGGGCAGGGGAAGGAGAGAAAGGGAGAGCAGGAACAAGAGAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=20,212
SEQUENCE_TARGET=86,58
P3_COMMENT=CL is CATGGGGAGGAGAGTTGAAA
P3_COMMENT=CR is CCCCTTCCCTTCTCTCTACG
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.238868
PRIMER_LEFT_0_PENALTY=0.042377
PRIMER_RIGHT_0_PENALTY=0.196491
PRIMER_LEFT_0_SEQUENCE=CATGGGGAGGAGAGTTGAAA
PRIMER_RIGHT_0_SEQUENCE=CCCCTTCCCTTCTCTCTACG
PRIMER_LEFT_0=62,20
PRIMER_RIGHT_0=189,20
PRIMER_LEFT_0_TM=60.042
PRIMER_RIGHT_0_TM=60.196
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=128
PRIMER_PAIR_0_PRODUCT_TM=68.7
PRIMER_PAIR_1_PENALTY=0.293572
PRIMER_LEFT_1_PENALTY=0.042377
PRIMER_RIGHT_1_PENALTY=0.251196
PRIMER_LEFT_1_SEQUENCE=CATGGGGAGGAGAGTTGAAA
PRIMER_RIGHT_1_SEQUENCE=TCTCCCTTTCTCTCCTTCCC
PRIMER_LEFT_1=62,20
PRIMER_RIGHT_1=212,20
PRIMER_LEFT_1_TM=60.042
PRIMER_RIGHT_1_TM=59.749
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=9.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PAIR_1_PRODUCT_SIZE=151
PRIMER_PAIR_1_PRODUCT_TM=70.9
PRIMER_PAIR_2_PENALTY=0.510463
PRIMER_LEFT_2_PENALTY=0.042377
PRIMER_RIGHT_2_PENALTY=0.468086
PRIMER_LEFT_2_SEQUENCE=CATGGGGAGGAGAGTTGAAA
PRIMER_RIGHT_2_SEQUENCE=CCTGCTCTCCCTTTCTCTCC
PRIMER_LEFT_2=62,20
PRIMER_RIGHT_2=217,20
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=60.468
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=156
PRIMER_PAIR_2_PRODUCT_TM=71.6
PRIMER_PAIR_3_PENALTY=0.512377
PRIMER_LEFT_3_PENALTY=0.042377
PRIMER_RIGHT_3_PENALTY=0.470001
PRIMER_LEFT_3_SEQUENCE=CATGGGGAGGAGAGTTGAAA
PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTTCCTGCTCTCCC
PRIMER_LEFT_3=62,20
PRIMER_RIGHT_3=226,20
PRIMER_LEFT_3_TM=60.042
PRIMER_RIGHT_3_TM=59.530
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=165
PRIMER_PAIR_3_PRODUCT_TM=71.7
PRIMER_PAIR_4_PENALTY=0.637090
PRIMER_LEFT_4_PENALTY=0.042377
PRIMER_RIGHT_4_PENALTY=0.594713
PRIMER_LEFT_4_SEQUENCE=CATGGGGAGGAGAGTTGAAA
PRIMER_RIGHT_4_SEQUENCE=TTGTTCCTGCTCTCCCTTTC
PRIMER_LEFT_4=62,20
PRIMER_RIGHT_4=222,20
PRIMER_LEFT_4_TM=60.042
PRIMER_RIGHT_4_TM=59.405
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=161
PRIMER_PAIR_4_PRODUCT_TM=71.5
=
SEQUENCE_ID=43.RHAP131FG10.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCATACACAGAACTAGGGTCTATACACAGTACTAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTACAGACATGAATGCATAGTGTAGGCATAAAAAGAAAGGCTCGATTTCATTCTTTTATCCACACTGTTCACCTGTGTCACCTGTTTTGAGCATGTGAGTCTGTTTTGAGCATGCTAGCCTGTATATAACTAGAGGAACTTAACAAGACTGCACAAGTGAACTCAGGGTAAGGACATATATAAAAGAACATTACAATAGGCAGGGTGAAGTGGGAACAGCAATCCCAGAACACATTCTGGAAATGTTGTAGTTTGAGGAGGAAGAGAGGGAAAGGTCACTCTGGGCATAATTTGGCTTTAACATCAATTTTCCTTCTGGTAGGAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=23,414
SEQUENCE_TARGET=60,46
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=47.RHAP131FH5.seq
SEQUENCE_TEMPLATE=ANNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTNGTGTCTGGGGGGAGGCTNCTCTCCTGTGTGNTGCAGCCANTGGGGTTGGGGAGGAGCANANAGTTTGCNTTNNTGGAGTGAATCAACCAAATTTTGGGAGTTGGAAGTATTTTATTANAAACATNTGGAGATTTTAGAATTTTTTNAAAATGTTGTTATTTAAANCCTTTTCAACGTTAGAAAGTAATAAGTAGGGGTGGGNGGNACGCCCTCNNAGGNNNTTGTTTCAAAANGNNTCNCCCAACTCCCATGANTTTCTTGGNTTTTTTTNNTTNCCGGNAACCNTGNTCTCCTNNAANATCAAAACCCATCAACTTTTGGNGGCCANAATAATCNNTTTTTACTANANTGNNTGNGNGTCCCTTGGTANANTNGNTTNNGGGGGTANCNGANCTCNANNTTCNNTAANTNATGGGNNAAANNGGTTTTTTTNCNTGGGGNNNANTTGTTTTNNTCTNNCCNNNNATTTTTNCNACATTGANANNNNGGNNCCCGGGGCGCNATTNNNGGTGTAANNNCCTGGGGGNNNNNTAANNGNGNGGNNNNNNCCTCANTTTTTANTGGGCGGTNNNCCNCNNNTGCCCNTTTTTTCTGNNTGGGGAAANNTTGTTNNNNNNNNTNG
SEQUENCE_INCLUDED_REGION=0,663
SEQUENCE_TARGET=6,29
P3_COMMENT=CL is 
P3_COMMENT=CR is 
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=48.RHAP131FH12.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCAGGCATGNGTGAGGGGATGAGAACCCACCTGCAATGTNAAGGAAGTGATGTGATTATTGTTCATGCCCATGTCTCATTCTTCTCCCTTTCCCATGTTGGTCCTGATGGAAATGGAAAAAATAGAGTTGGGGGAAACTTGGAGACCTTATTGTAATATTAGAGACTATGAGGAATCTTAGGATTCTTTTGGGAAAGGCTTGGGCATGTCTTATGATAGATTTCAAGCTAGTAACAACACATCCAAGGATGCTTCTTAAGAAATTTAATTAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATTCAGGTCAACATAGTTGCTTGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
SEQUENCE_INCLUDED_REGION=21,348
SEQUENCE_TARGET=297,40
P3_COMMENT=CL is AGGCTTGGGCATGTCTTATG
P3_COMMENT=CR is TCCACAAGCAACTATGTTGACC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.133110
PRIMER_LEFT_0_PENALTY=0.095771
PRIMER_RIGHT_0_PENALTY=2.037339
PRIMER_LEFT_0_SEQUENCE=AGGCTTGGGCATGTCTTATG
PRIMER_RIGHT_0_SEQUENCE=TCCACAAGCAACTATGTTGACC
PRIMER_LEFT_0=215,20
PRIMER_RIGHT_0=366,22
PRIMER_LEFT_0_TM=60.096
PRIMER_RIGHT_0_TM=60.037
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=152
PRIMER_PAIR_0_PRODUCT_TM=66.2
PRIMER_PAIR_1_PENALTY=2.263884
PRIMER_LEFT_1_PENALTY=0.095771
PRIMER_RIGHT_1_PENALTY=2.168114
PRIMER_LEFT_1_SEQUENCE=AGGCTTGGGCATGTCTTATG
PRIMER_RIGHT_1_SEQUENCE=CCTCCACAAGCAACTATGTTGA
PRIMER_LEFT_1=215,20
PRIMER_RIGHT_1=368,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.168
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=154
PRIMER_PAIR_1_PRODUCT_TM=66.7
PRIMER_PAIR_2_PENALTY=2.329753
PRIMER_LEFT_2_PENALTY=0.292414
PRIMER_RIGHT_2_PENALTY=2.037339
PRIMER_LEFT_2_SEQUENCE=AAAGGCTTGGGCATGTCTTA
PRIMER_RIGHT_2_SEQUENCE=TCCACAAGCAACTATGTTGACC
PRIMER_LEFT_2=213,20
PRIMER_RIGHT_2=366,22
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=154
PRIMER_PAIR_2_PRODUCT_TM=66.1
PRIMER_PAIR_3_PENALTY=2.460528
PRIMER_LEFT_3_PENALTY=0.292414
PRIMER_RIGHT_3_PENALTY=2.168114
PRIMER_LEFT_3_SEQUENCE=AAAGGCTTGGGCATGTCTTA
PRIMER_RIGHT_3_SEQUENCE=CCTCCACAAGCAACTATGTTGA
PRIMER_LEFT_3=213,20
PRIMER_RIGHT_3=368,22
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=60.168
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=156
PRIMER_PAIR_3_PRODUCT_TM=66.6
PRIMER_PAIR_4_PENALTY=2.505221
PRIMER_LEFT_4_PENALTY=0.467882
PRIMER_RIGHT_4_PENALTY=2.037339
PRIMER_LEFT_4_SEQUENCE=GGATTCTTTTGGGAAAGGCT
PRIMER_RIGHT_4_SEQUENCE=TCCACAAGCAACTATGTTGACC
PRIMER_LEFT_4=200,20
PRIMER_RIGHT_4=366,22
PRIMER_LEFT_4_TM=59.532
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=167
PRIMER_PAIR_4_PRODUCT_TM=67.1
=
