SEQUENCE_ID=io_gc_content
PRIMER_WT_SEQ_QUAL=0
PRIMER_INTERNAL_WT_SEQ_QUAL=0
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_INTERNAL_MIN_TM=0
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_GC=40
PRIMER_INTERNAL_MAX_GC=60
PRIMER_INTERNAL_WT_GC_PERCENT_GT=1
PRIMER_INTERNAL_WT_GC_PERCENT_LT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PAIR_WT_IO_PENALTY=1
PRIMER_PAIR_WT_PR_PENALTY=0
PRIMER_WT_TM_GT=0
PRIMER_WT_TM_LT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_SIZE_GT=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_WT_TM_GT=0
PRIMER_INTERNAL_WT_TM_LT=0
PRIMER_INTERNAL_WT_SIZE_LT=0
PRIMER_INTERNAL_WT_SIZE_GT=0
PRIMER_INTERNAL_WT_SELF_ANY=0
PRIMER_INTERNAL_WT_SELF_END=0
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
PRIMER_ERROR=Hyb probe GC content is part of objective function while optimum gc_content is not defined
=
