P3_COMMENT=Testing PRIMER_MAX_POLY_X
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_NS_ACCEPTED=100
PRIMER_MAX_TM=99
PRIMER_MIN_TM=50
PRIMER_TASK=check_primers
SEQUENCE_ID=primer3_poly_x_01
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CNCNCCATTGAGTAGCAAGG
P3_COMMENT=CNCNCC - need to join disjoint N regions, fails on poly_x
=
SEQUENCE_ID=primer3_poly_x_02
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=NNNNNCAATTGAGTAGCAAGG
P3_COMMENT=Nx5 at 5' end, fails on poly_x
=
SEQUENCE_ID=primer3_poly_x_03
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CCATCTGAGTAGCAGAGGNNNN
P3_COMMENT=GGNNNN at 3' end, fails on poly_x
=
SEQUENCE_ID=primer3_poly_x_04
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=ANCNCCATTGAGTAGCAAGG
P3_COMMENT=CNCNNG disjoint N regions with different end bp where 3' end bp
P3_COMMENT=is the same as bp between Ns - returns 1 primer
=
SEQUENCE_ID=primer3_poly_x_05
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CNCNNGCATTGAGTAGCAAGG
P3_COMMENT=CNCNNG disjoint N regions with different end bp where 5' end bp
P3_COMMENT=is the same as bp between Ns - returns 1 primer
=
SEQUENCE_ID=primer3_poly_x_06
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CCCNCCCACATTGAGTAGCAAGG
P3_COMMENT=CCCNCCC, fails on poly_x
=
SEQUENCE_ID=primer3_poly_x_07
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CNNNNGACATTGAGTAGCAAGG
P3_COMMENT=CNNNNG, returns 1 primer
=
SEQUENCE_ID=primer3_poly_x_08
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CCCCCGACATTGAGTAGCAAGG
P3_COMMENT=Cx5, returns 1 primer
=
SEQUENCE_ID=primer3_poly_x_09
PRIMER_MAX_POLY_X=5
SEQUENCE_PRIMER=CCCCCCGACATTGAGTAGCAAGG
P3_COMMENT=Cx6, fails on poly_x
=
SEQUENCE_ID=primer3_poly_x_10
PRIMER_MAX_POLY_X=10
SEQUENCE_PRIMER=CCCCCCGACATTGAGTAGCAAGG
P3_COMMENT=Cx6, returns 1 primer
=
