SEQUENCE_ID=template_mis_1
PRIMER_NUM_RETURN=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.934085
PRIMER_LEFT_0_PENALTY=0.323851
PRIMER_RIGHT_0_PENALTY=0.610234
PRIMER_LEFT_0_SEQUENCE=Gatgcgtacgatccatgcta
PRIMER_RIGHT_0_SEQUENCE=tagcggcctagtgtgtgtgt
PRIMER_LEFT_0=20,20
PRIMER_RIGHT_0=131,20
PRIMER_LEFT_0_TM=59.676
PRIMER_RIGHT_0_TM=59.390
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.5000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=6.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=6.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=112
PRIMER_PAIR_0_PRODUCT_TM=71.8
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=12.00
=
SEQUENCE_ID=template_mis_2
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG
PRIMER_WARNING=More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_EXPLAIN=considered 1240, low tm 698, high tm 374, high any compl 13, high end compl 34, ok 121
PRIMER_PAIR_EXPLAIN=considered 242, ok 242
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2600.116621
PRIMER_LEFT_0_PENALTY=0.102704
PRIMER_RIGHT_0_PENALTY=0.013916
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high;
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_0_SEQUENCE=gatatCAGTGTGTACATGGCGG
PRIMER_LEFT_0=32,23
PRIMER_RIGHT_0=197,22
PRIMER_LEFT_0_TM=62.054
PRIMER_RIGHT_0_TM=60.278
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=12.9000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=17.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=166
PRIMER_PAIR_0_PRODUCT_TM=74.1
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=26.00
=
SEQUENCE_ID=template_mis_3
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC
PRIMER_WARNING=More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat
PRIMER_PAIR_EXPLAIN=considered 2, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2601.096874
PRIMER_LEFT_0_PENALTY=0.102704
PRIMER_RIGHT_0_PENALTY=0.994170
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high;
PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract;
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG
PRIMER_LEFT_0=32,23
PRIMER_RIGHT_0=240,18
PRIMER_LEFT_0_TM=62.054
PRIMER_RIGHT_0_TM=79.883
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=3.0000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=209
PRIMER_PAIR_0_PRODUCT_TM=77.4
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=26.00
=
SEQUENCE_ID=template_mis_4
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA
PRIMER_WARNING=Unrecognized base in input sequence; More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat
PRIMER_PAIR_EXPLAIN=considered 2, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2701.096874
PRIMER_LEFT_0_PENALTY=0.102704
PRIMER_RIGHT_0_PENALTY=0.994170
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high;
PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract;
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG
PRIMER_LEFT_0=23,23
PRIMER_RIGHT_0=231,18
PRIMER_LEFT_0_TM=62.054
PRIMER_RIGHT_0_TM=79.883
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=16.0000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=209
PRIMER_PAIR_0_PRODUCT_TM=77.4
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=27.00
=
SEQUENCE_ID=template_mis_5
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA
PRIMER_WARNING=More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat
PRIMER_PAIR_EXPLAIN=considered 2, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2701.096874
PRIMER_LEFT_0_PENALTY=0.102704
PRIMER_RIGHT_0_PENALTY=0.994170
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high;
PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract;
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG
PRIMER_LEFT_0=23,23
PRIMER_RIGHT_0=231,18
PRIMER_LEFT_0_TM=62.054
PRIMER_RIGHT_0_TM=79.883
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=16.0000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=209
PRIMER_PAIR_0_PRODUCT_TM=77.4
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=27.00
=
SEQUENCE_ID=template_mis_6
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1901.096874
PRIMER_LEFT_0_PENALTY=0.102704
PRIMER_RIGHT_0_PENALTY=0.994170
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high;
PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract;
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG
PRIMER_LEFT_0=0,23
PRIMER_RIGHT_0=67,18
PRIMER_LEFT_0_TM=62.054
PRIMER_RIGHT_0_TM=79.883
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=3.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=16.0000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=68
PRIMER_PAIR_0_PRODUCT_TM=80.6
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=19.00
=
SEQUENCE_ID=template_mis_7
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1276.096874
PRIMER_LEFT_0_PENALTY=0.102704
PRIMER_RIGHT_0_PENALTY=0.994170
PRIMER_LEFT_0_PROBLEMS= Similarity to self too high;
PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract;
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG
PRIMER_LEFT_0=0,23
PRIMER_RIGHT_0=67,18
PRIMER_LEFT_0_TM=62.054
PRIMER_RIGHT_0_TM=79.883
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=3.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=9.7500
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=68
PRIMER_PAIR_0_PRODUCT_TM=75.8
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=12.75
=
SEQUENCE_ID=template_mis_8
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=11
PRIMER_MAX_POLY_X=40
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
P3_COMMENT=Should succeed
PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, ok 20
PRIMER_PAIR_EXPLAIN=considered 20, high any compl 11, high end compl 5, ok 4
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1076.079233
PRIMER_LEFT_0_PENALTY=0.085063
PRIMER_RIGHT_0_PENALTY=0.994170
PRIMER_LEFT_0_SEQUENCE=CCCCCCCCAAAAAAAAAA
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG
PRIMER_LEFT_0=32,18
PRIMER_RIGHT_0=67,18
PRIMER_LEFT_0_TM=61.701
PRIMER_RIGHT_0_TM=79.883
PRIMER_LEFT_0_GC_PERCENT=44.444
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=0.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=4.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=9.7500
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=36
PRIMER_PAIR_0_PRODUCT_TM=72.8
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=10.75
=
SEQUENCE_ID=template_mis_9
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=9
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
P3_COMMENT=Should succeed
PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, ok 20
PRIMER_RIGHT_EXPLAIN=considered 177, too many Ns 22, high template mispriming score 5, ok 150
PRIMER_PAIR_EXPLAIN=considered 3004, unacceptable product size 2784, high any compl 199, ok 21
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=776.116327
PRIMER_LEFT_0_PENALTY=0.085063
PRIMER_RIGHT_0_PENALTY=1.031264
PRIMER_LEFT_0_SEQUENCE=CCCCCCCCAAAAAAAAAA
PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGTTTTT
PRIMER_LEFT_0=32,18
PRIMER_RIGHT_0=67,23
PRIMER_LEFT_0_TM=61.701
PRIMER_RIGHT_0_TM=80.625
PRIMER_LEFT_0_GC_PERCENT=44.444
PRIMER_RIGHT_0_GC_PERCENT=78.261
PRIMER_LEFT_0_SELF_ANY=0.00
PRIMER_RIGHT_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=4.0000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=6.7500
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=36
PRIMER_PAIR_0_PRODUCT_TM=72.8
PRIMER_PAIR_0_TEMPLATE_MISPRIMING=7.75
=
SEQUENCE_ID=template_mis_10
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=6.7
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
P3_COMMENT=Should fail
PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, high template mispriming score 2, ok 18
PRIMER_RIGHT_EXPLAIN=considered 177, too many Ns 22, high template mispriming score 19, ok 136
PRIMER_PAIR_EXPLAIN=considered 2718, unacceptable product size 2710, high any compl 8, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=template_mis_11
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.732142
PRIMER_LEFT_0_SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGc
PRIMER_LEFT_0=25,26
PRIMER_LEFT_0_TM=74.643
PRIMER_LEFT_0_GC_PERCENT=57.692
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=20.0000
=
SEQUENCE_ID=template_mis_12
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_INCLUDED_REGION=25,26
SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo plus former error in ignoring included_region offset
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.732142
PRIMER_LEFT_0_SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGc
PRIMER_LEFT_0=25,26
PRIMER_LEFT_0_TM=74.643
PRIMER_LEFT_0_GC_PERCENT=57.692
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=20.0000
=
SEQUENCE_ID=template_mis_13
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=cTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGc
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.732142
PRIMER_LEFT_0_SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGc
PRIMER_LEFT_0=0,26
PRIMER_LEFT_0_TM=74.643
PRIMER_LEFT_0_GC_PERCENT=57.692
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=22.0000
=
SEQUENCE_ID=template_mis_14
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=GCCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAT
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_11
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.732142
PRIMER_RIGHT_0_SEQUENCE=CTTGATGGAGAATTGCGCAGAGGGGC
PRIMER_RIGHT_0=25,26
PRIMER_RIGHT_0_TM=74.643
PRIMER_RIGHT_0_GC_PERCENT=57.692
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=20.0000
=
SEQUENCE_ID=template_mis_15
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.732142
PRIMER_RIGHT_0_SEQUENCE=CTTGATGGAGAATTGCGCAGAGGGGC
PRIMER_RIGHT_0=49,26
PRIMER_RIGHT_0_TM=74.643
PRIMER_RIGHT_0_GC_PERCENT=57.692
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=22.0000
=
SEQUENCE_ID=template_mis_16
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_INCLUDED_REGION=24,26
SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.732142
PRIMER_RIGHT_0_SEQUENCE=CTTGATGGAGAATTGCGCAGAGGGGC
PRIMER_RIGHT_0=49,26
PRIMER_RIGHT_0_TM=74.643
PRIMER_RIGHT_0_GC_PERCENT=57.692
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=22.0000
=
SEQUENCE_ID=short_align_target_1
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_SELF_ANY=0.0
PRIMER_WT_SELF_END=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_LIBRARY_MISPRIMING=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY=0.0
PRIMER_PAIR_WT_COMPL_END=0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_INTERNAL_MIN_SIZE=18
PRIMER_INTERNAL_OPT_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=27
PRIMER_INTERNAL_MIN_TM=57.0
PRIMER_INTERNAL_OPT_TM=60.0
PRIMER_INTERNAL_MAX_TM=63.0
PRIMER_INTERNAL_MIN_GC=20.0
PRIMER_INTERNAL_MAX_GC=80.0
PRIMER_INTERNAL_MAX_SELF_ANY=12.00
PRIMER_INTERNAL_MAX_SELF_END=12.00
PRIMER_INTERNAL_MAX_NS_ACCEPTED=0
PRIMER_INTERNAL_MAX_POLY_X=5
PRIMER_INTERNAL_MISHYB_LIBRARY=
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00
PRIMER_INTERNAL_MIN_QUALITY=0
PRIMER_INTERNAL_SALT_MONOVALENT=50.0
PRIMER_INTERNAL_DNA_CONC=50.0
PRIMER_INTERNAL_SALT_DIVALENT=0.0
PRIMER_INTERNAL_DNTP_CONC=0.0
PRIMER_INTERNAL_WT_TM_LT=1.0
PRIMER_INTERNAL_WT_TM_GT=1.0
PRIMER_INTERNAL_WT_SIZE_LT=1.0
PRIMER_INTERNAL_WT_SIZE_GT=1.0
PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0
PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0
PRIMER_INTERNAL_WT_SELF_ANY=0.0
PRIMER_INTERNAL_WT_NUM_NS=0.0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0
PRIMER_INTERNAL_WT_SEQ_QUAL=0.0
PRIMER_TASK=pick_right_only
PRIMER_FIRST_BASE_INDEX=1
PRIMER_PICK_ANYWAY=1
P3_COMMENT=Test for very short target of alignment number 1
PRIMER_ERROR=Missing SEQUENCE tag
=
SEQUENCE_ID=short_align_target_2
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=
SEQUENCE_TEMPLATE=ACCAAGAAAGTAGTTGACAAAAAAATGAAAACCACTATTATTTAAATTACGGAAAAAAAAAAACCAATAAACAAAAAACATAATAAAAGAAATGAAAAAAAAGAATATTAGTAGAGCATACTGAATTGTTTTCAGAAAGAGAGTGAAAGGTAACAAAAATTTACCTACTTTTCCTTTTTATTTCTCTGGTTTCGTTTCTTTTTTTTCGGCCACCCTATTTATTTTTTCACTGCTTGTAAACAACGTCTCACTTCAATAGTGTCTCCTTCATGTACTGAGATACTGCTGCAAGCGAAATAT
PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000
PRIMER_NUM_RETURN=5
PRIMER_MAX_END_STABILITY=9.0
PRIMER_MAX_LIBRARY_MISPRIMING=12.00
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00
PRIMER_MAX_TEMPLATE_MISPRIMING=12.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00
PRIMER_MIN_SIZE=18
PRIMER_OPT_SIZE=20
PRIMER_MAX_SIZE=27
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_TM=63.0
PRIMER_PAIR_MAX_DIFF_TM=100.0
PRIMER_TM_FORMULA=0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_MAX_SELF_ANY=8.00
PRIMER_MAX_SELF_END=3.00
PRIMER_PAIR_MAX_COMPL_ANY=8.00
PRIMER_PAIR_MAX_COMPL_END=3.00
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_POLY_X=5
PRIMER_OUTSIDE_PENALTY=0
PRIMER_GC_CLAMP=0
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_CORRECTIONS=0
PRIMER_SALT_DIVALENT=0.0
PRIMER_DNTP_CONC=0.0
PRIMER_DNA_CONC=50.0
PRIMER_LIBERAL_BASE=1
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_MIN_QUALITY=0
PRIMER_MIN_END_QUALITY=0
PRIMER_QUALITY_RANGE_MIN=0
PRIMER_QUALITY_RANGE_MAX=100
PRIMER_WT_TM_LT=1.0
PRIMER_WT_TM_GT=1.0
PRIMER_WT_SIZE_LT=1.0
PRIMER_WT_SIZE_GT=1.0
PRIMER_WT_GC_PERCENT_LT=0.0
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_SELF_ANY=0.0
PRIMER_WT_SELF_END=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_LIBRARY_MISPRIMING=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY=0.0
PRIMER_PAIR_WT_COMPL_END=0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_INTERNAL_MIN_SIZE=18
PRIMER_INTERNAL_OPT_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=27
PRIMER_INTERNAL_MIN_TM=57.0
PRIMER_INTERNAL_OPT_TM=60.0
PRIMER_INTERNAL_MAX_TM=63.0
PRIMER_INTERNAL_MIN_GC=20.0
PRIMER_INTERNAL_MAX_GC=80.0
PRIMER_INTERNAL_MAX_SELF_ANY=12.00
PRIMER_INTERNAL_MAX_SELF_END=12.00
PRIMER_INTERNAL_MAX_NS_ACCEPTED=0
PRIMER_INTERNAL_MAX_POLY_X=5
PRIMER_INTERNAL_MISHYB_LIBRARY=
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00
PRIMER_INTERNAL_MIN_QUALITY=0
PRIMER_INTERNAL_SALT_MONOVALENT=50.0
PRIMER_INTERNAL_DNA_CONC=50.0
PRIMER_INTERNAL_SALT_DIVALENT=0.0
PRIMER_INTERNAL_DNTP_CONC=0.0
PRIMER_INTERNAL_WT_TM_LT=1.0
PRIMER_INTERNAL_WT_TM_GT=1.0
PRIMER_INTERNAL_WT_SIZE_LT=1.0
PRIMER_INTERNAL_WT_SIZE_GT=1.0
PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0
PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0
PRIMER_INTERNAL_WT_SELF_ANY=0.0
PRIMER_INTERNAL_WT_NUM_NS=0.0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0
PRIMER_INTERNAL_WT_SEQ_QUAL=0.0
PRIMER_TASK=pick_left_only
PRIMER_FIRST_BASE_INDEX=1
PRIMER_PICK_ANYWAY=1
P3_COMMENT=Test for very short target of alignment number 1
PRIMER_LEFT_EXPLAIN=considered 2141, GC content failed 553, low tm 1093, high tm 44, high any compl 1, high end compl 75, long poly-x seq 91, high 3' stability 6, high template mispriming score 9, ok 269
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.571405
PRIMER_LEFT_0_SEQUENCE=TTTTTCGGCCACCCTATTTA
PRIMER_LEFT_0=202,20
PRIMER_LEFT_0_TM=59.429
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.2000
PRIMER_LEFT_0_TEMPLATE_MISPRIMING=6.0000
PRIMER_LEFT_1_PENALTY=1.175605
PRIMER_LEFT_1_SEQUENCE=AGATACTGCTGCAAGCGAAA
PRIMER_LEFT_1=278,20
PRIMER_LEFT_1_TM=58.824
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_LEFT_1_TEMPLATE_MISPRIMING=7.0000
PRIMER_LEFT_2_PENALTY=1.299895
PRIMER_LEFT_2_SEQUENCE=TCGGCCACCCTATTTATTTTT
PRIMER_LEFT_2=206,21
PRIMER_LEFT_2_TM=59.700
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_LEFT_2_TEMPLATE_MISPRIMING=9.0000
PRIMER_LEFT_3_PENALTY=1.299895
PRIMER_LEFT_3_SEQUENCE=TTCGGCCACCCTATTTATTTT
PRIMER_LEFT_3=205,21
PRIMER_LEFT_3_TM=59.700
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_LEFT_3_TEMPLATE_MISPRIMING=8.0000
PRIMER_LEFT_4_PENALTY=1.299895
PRIMER_LEFT_4_SEQUENCE=TTTCGGCCACCCTATTTATTT
PRIMER_LEFT_4=204,21
PRIMER_LEFT_4_TM=59.700
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_LEFT_4_TEMPLATE_MISPRIMING=9.0000
=
