PRIMER PICKING RESULTS FOR anyway_1

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left      10     0     0     0     0     0     0     7     0     0     0     0     0     3
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR high_tm

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR high_tm_and_self_any

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high/High self complementarity

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_target

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Overlaps Target/Tm too high/High self complementarity

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 20,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>)***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ********************** <<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Right      3     0     0     0     0     0     0     0     2     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_excl_region

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Overlaps Excluded Region/Tm too high/High self complementarity

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122
EXCLUDED REGIONS (start, len)*: 20,1

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>X***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
XXXXXX excluded region
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_incl_region

INPUT PROBLEM: Specified left primer not in Included Region

PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high/High self complementarity

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right_bad_gc

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Unacceptable GC content

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right_bad_gc_and_target

No mispriming library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122 143,1

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************(<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR internal_too_short

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: Specified internal oligo < PRIMER_INTERNAL_MIN_SIZE; Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Too short/Tm too low

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
INTERNAL OLIGO      23   13   41.12   53.85  6.00  2.00 gcgtacgatccat
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122 143,1

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************(<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR internal_ok

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
INTERNAL OLIGO      23   19   59.42   57.89  6.00  6.00 gcgtacgatccatgctagc
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122 143,1

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************(<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR internal_too_cold

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Tm too low

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   21   60.12   47.62 12.00  0.00 TCCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
INTERNAL OLIGO      23   19   59.42   57.89  6.00  6.00 gcgtacgatccatgctagc
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122 143,1

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
      >>>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************(<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR internal_too_cold2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: Hybridization probe is unacceptable: Tm too low

OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   58.23   50.00 10.00  0.00 CCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00 CAGTGTGTACATGGCGGC
INTERNAL OLIGO      23   19   59.42   57.89  6.00  6.00 gcgtacgatccatgctagc
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
       >>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR mispriming

Using mispriming library not_for_production.lib
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: Left primer is unacceptable: High similarity to mispriming or mishyb library; Hybridization probe is unacceptable: Tm too low

OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          1   20   58.23   50.00 10.00  0.00  9.00 CCTACAGCTGTGGGAAAATG
RIGHT PRIMER       160   18   59.69   61.11  6.00  3.00  8.00 CAGTGTGTACATGGCGGC
INTERNAL OLIGO      23   19   59.42   57.89  6.00  6.00       gcgtacgatccatgctagc
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 21,122

    0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
       >>>>>>>>>>>>>>>>>>>>***************************************

   60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
      ************************************************************

  120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
      ***********************<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


