P3_SETTINGS_FILE_USED=../th-w-other-tasks-settings.txt
P3_FILE_TYPE=settings
PRIMER_FIRST_BASE_INDEX=1
PRIMER_TASK=pick_pcr_primers
P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt
PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 200-299 501-600 100-199 601-700 701-850 851-1001
PRIMER_NUM_RETURN=5
PRIMER_MAX_END_STABILITY=9.0
PRIMER_MAX_LIBRARY_MISPRIMING=12.00
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00
PRIMER_MAX_SELF_ANY_TH=47.00
PRIMER_MAX_SELF_END_TH=47.00
PRIMER_MAX_HAIRPIN_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00
PRIMER_PAIR_MAX_COMPL_END_TH=47.00
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00
PRIMER_MIN_SIZE=18
PRIMER_OPT_SIZE=20
PRIMER_MAX_SIZE=23
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=59.0
PRIMER_MAX_TM=63.0
PRIMER_PAIR_MAX_DIFF_TM=5.0
PRIMER_TM_FORMULA=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_DIVALENT=1.5
PRIMER_DNTP_CONC=0.6
PRIMER_DNA_CONC=50.0
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=../primer3_config/
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_LOWERCASE_MASKING=0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_POLY_X=5
PRIMER_OUTSIDE_PENALTY=0
PRIMER_GC_CLAMP=0
PRIMER_LIBERAL_BASE=1
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_WT_TM_LT=1.0
PRIMER_WT_TM_GT=1.0
PRIMER_WT_SIZE_LT=1.0
PRIMER_WT_SIZE_GT=1.0
PRIMER_WT_GC_PERCENT_LT=0.0
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_SELF_ANY_TH=0.0
PRIMER_WT_SELF_END_TH=0.0
PRIMER_WT_HAIRPIN_TH=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_LIBRARY_MISPRIMING=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY_TH=0.0
PRIMER_PAIR_WT_COMPL_END_TH=0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PICK_ANYWAY=0
P3_SETTINGS_FILE_END=
PRIMER_TASK=generic
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=19
PRIMER_MAX_SIZE=21
PRIMER_NUM_RETURN=1
PRIMER_MAX_SELF_ANY_TH=47.00
PRIMER_MAX_SELF_END_TH=47.00
PRIMER_MAX_HAIRPIN_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00
PRIMER_PAIR_MAX_COMPL_END_TH=47.00
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt
SEQUENCE_ID=pick_detection_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 45, too many Ns 3, low tm 28, high repeat similarity 1, ok 13
PRIMER_RIGHT_EXPLAIN=considered 90, low tm 36, high tm 12, ok 42
PRIMER_PAIR_EXPLAIN=considered 18, unacceptable product size 11, high mispriming library similarity 4, ok 3
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1.608735
PRIMER_LEFT_0_PENALTY=1.001812
PRIMER_RIGHT_0_PENALTY=0.606923
PRIMER_LEFT_0_SEQUENCE=AGACATGACACTGACGATGC
PRIMER_RIGHT_0_SEQUENCE=AGCTATCGATTTGGGTCGGG
PRIMER_LEFT_0=10,20
PRIMER_RIGHT_0=97,20
PRIMER_LEFT_0_TM=57.998
PRIMER_RIGHT_0_TM=59.607
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=1.71
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=41.74
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_0_END_STABILITY=3.9100
PRIMER_RIGHT_0_END_STABILITY=5.1400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=1.96
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_0_PRODUCT_TM=82.1
PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=11.40
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_detection_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 184, too many Ns 5, GC content failed 1, low tm 140, long poly-x seq 16, ok 22
PRIMER_RIGHT_EXPLAIN=considered 216, low tm 167, high tm 13, ok 36
PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2.181798
PRIMER_LEFT_0_PENALTY=1.695359
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_LEFT_0_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_LEFT_0=53,21
PRIMER_RIGHT_0=137,20
PRIMER_LEFT_0_TM=58.305
PRIMER_RIGHT_0_TM=58.514
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=3.7700
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_PAIR_0_COMPL_ANY_TH=10.83
PRIMER_PAIR_0_COMPL_END_TH=1.44
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_0_PRODUCT_TM=78.8
PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=15.19
=
PRIMER_TASK=pick_left_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_left_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.527529
PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC
PRIMER_LEFT_0=61,20
PRIMER_LEFT_0_TM=59.528
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=10.70
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=40.47
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=4.1902
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_left_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_LEFT_0=118,20
PRIMER_LEFT_0_TM=58.514
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
=
PRIMER_TASK=pick_right_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_right_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 17, high repeat similarity 3, ok 34
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.527529
PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA
PRIMER_RIGHT_0=80,20
PRIMER_RIGHT_0_TM=59.528
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=41.64
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_RIGHT_0_END_STABILITY=2.2400
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_right_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_RIGHT_0=137,20
PRIMER_RIGHT_0_TM=58.514
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
=
PRIMER_TASK=pick_hyb_probe_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_hyb_probe_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_0=66,20
PRIMER_INTERNAL_0_TM=59.273
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_hyb_probe_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_INTERNAL_0=125,20
PRIMER_INTERNAL_0_TM=59.906
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
=
PRIMER_TASK=pick_primer_list
PRIMER_NUM_RETURN=10000
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_primer_list_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=168
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_0=66,20
PRIMER_INTERNAL_0_TM=59.273
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_INTERNAL_1_PENALTY=1.416589
PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_1=64,21
PRIMER_INTERNAL_1_TM=59.583
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_SELF_ANY_TH=7.03
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=41.60
PRIMER_INTERNAL_2_PENALTY=1.438741
PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_2=67,20
PRIMER_INTERNAL_2_TM=58.561
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.60
PRIMER_INTERNAL_3_PENALTY=1.438741
PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_3=65,20
PRIMER_INTERNAL_3_TM=58.561
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=41.60
PRIMER_INTERNAL_4_PENALTY=1.650306
PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_4=66,21
PRIMER_INTERNAL_4_TM=60.650
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_INTERNAL_4_SELF_ANY_TH=2.18
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=41.60
PRIMER_INTERNAL_5_PENALTY=1.650306
PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_5=65,21
PRIMER_INTERNAL_5_TM=60.650
PRIMER_INTERNAL_5_GC_PERCENT=66.667
PRIMER_INTERNAL_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=41.60
PRIMER_INTERNAL_6_PENALTY=2.095978
PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_6=67,21
PRIMER_INTERNAL_6_TM=58.904
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=41.60
PRIMER_INTERNAL_7_PENALTY=2.360266
PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_7=63,21
PRIMER_INTERNAL_7_TM=58.640
PRIMER_INTERNAL_7_GC_PERCENT=61.905
PRIMER_INTERNAL_7_SELF_ANY_TH=7.03
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=41.60
PRIMER_INTERNAL_8_PENALTY=2.456420
PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_8=63,20
PRIMER_INTERNAL_8_TM=57.544
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_INTERNAL_8_SELF_ANY_TH=7.03
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=39.49
PRIMER_INTERNAL_9_PENALTY=2.511372
PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_9=64,20
PRIMER_INTERNAL_9_TM=57.489
PRIMER_INTERNAL_9_GC_PERCENT=65.000
PRIMER_INTERNAL_9_SELF_ANY_TH=7.03
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=41.60
PRIMER_INTERNAL_10_PENALTY=2.626619
PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_10=63,22
PRIMER_INTERNAL_10_TM=60.627
PRIMER_INTERNAL_10_GC_PERCENT=63.636
PRIMER_INTERNAL_10_SELF_ANY_TH=7.03
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.60
PRIMER_INTERNAL_11_PENALTY=2.783921
PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_11=67,22
PRIMER_INTERNAL_11_TM=59.216
PRIMER_INTERNAL_11_GC_PERCENT=59.091
PRIMER_INTERNAL_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_INTERNAL_11_HAIRPIN_TH=41.60
PRIMER_INTERNAL_12_PENALTY=2.885177
PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_12=66,22
PRIMER_INTERNAL_12_TM=60.885
PRIMER_INTERNAL_12_GC_PERCENT=63.636
PRIMER_INTERNAL_12_SELF_ANY_TH=2.18
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.60
PRIMER_INTERNAL_13_PENALTY=3.114431
PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_13=62,23
PRIMER_INTERNAL_13_TM=60.114
PRIMER_INTERNAL_13_GC_PERCENT=60.870
PRIMER_INTERNAL_13_SELF_ANY_TH=7.03
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_INTERNAL_13_HAIRPIN_TH=41.60
PRIMER_INTERNAL_14_PENALTY=3.510595
PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_14=64,22
PRIMER_INTERNAL_14_TM=61.511
PRIMER_INTERNAL_14_GC_PERCENT=68.182
PRIMER_INTERNAL_14_SELF_ANY_TH=7.03
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=41.60
PRIMER_INTERNAL_15_PENALTY=3.685489
PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_15=67,23
PRIMER_INTERNAL_15_TM=59.315
PRIMER_INTERNAL_15_GC_PERCENT=56.522
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=41.60
PRIMER_INTERNAL_16_PENALTY=3.807618
PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_16=62,22
PRIMER_INTERNAL_16_TM=58.192
PRIMER_INTERNAL_16_GC_PERCENT=59.091
PRIMER_INTERNAL_16_SELF_ANY_TH=7.03
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=41.60
PRIMER_INTERNAL_17_PENALTY=3.826170
PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_17=68,21
PRIMER_INTERNAL_17_TM=57.174
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_INTERNAL_17_HAIRPIN_TH=41.60
PRIMER_INTERNAL_18_PENALTY=3.864865
PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT
PRIMER_INTERNAL_18=37,23
PRIMER_INTERNAL_18_TM=59.135
PRIMER_INTERNAL_18_GC_PERCENT=52.174
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_PENALTY=3.881423
PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_19=62,21
PRIMER_INTERNAL_19_TM=57.119
PRIMER_INTERNAL_19_GC_PERCENT=57.143
PRIMER_INTERNAL_19_SELF_ANY_TH=7.03
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=39.49
PRIMER_INTERNAL_20_PENALTY=3.936624
PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_20=65,22
PRIMER_INTERNAL_20_TM=61.937
PRIMER_INTERNAL_20_GC_PERCENT=63.636
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=3.99
PRIMER_INTERNAL_20_HAIRPIN_TH=42.67
PRIMER_INTERNAL_21_PENALTY=3.956741
PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_INTERNAL_21=67,19
PRIMER_INTERNAL_21_TM=57.043
PRIMER_INTERNAL_21_GC_PERCENT=68.421
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.60
PRIMER_INTERNAL_22_PENALTY=3.956741
PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_INTERNAL_22=66,19
PRIMER_INTERNAL_22_TM=57.043
PRIMER_INTERNAL_22_GC_PERCENT=68.421
PRIMER_INTERNAL_22_SELF_ANY_TH=2.18
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=41.60
PRIMER_INTERNAL_23_PENALTY=4.001118
PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT
PRIMER_INTERNAL_23=36,24
PRIMER_INTERNAL_23_TM=60.001
PRIMER_INTERNAL_23_GC_PERCENT=54.167
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_24_PENALTY=4.001118
PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG
PRIMER_INTERNAL_24=35,24
PRIMER_INTERNAL_24_TM=60.001
PRIMER_INTERNAL_24_GC_PERCENT=54.167
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_INTERNAL_24_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_PENALTY=4.058186
PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_25=67,24
PRIMER_INTERNAL_25_TM=59.942
PRIMER_INTERNAL_25_GC_PERCENT=58.333
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=41.60
PRIMER_INTERNAL_26_PENALTY=4.099823
PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_26=66,23
PRIMER_INTERNAL_26_TM=61.100
PRIMER_INTERNAL_26_GC_PERCENT=60.870
PRIMER_INTERNAL_26_SELF_ANY_TH=2.18
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=41.60
PRIMER_INTERNAL_27_PENALTY=4.159384
PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT
PRIMER_INTERNAL_27=38,22
PRIMER_INTERNAL_27_TM=57.841
PRIMER_INTERNAL_27_GC_PERCENT=54.545
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_28_PENALTY=4.159384
PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG
PRIMER_INTERNAL_28=37,22
PRIMER_INTERNAL_28_TM=57.841
PRIMER_INTERNAL_28_GC_PERCENT=54.545
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_PENALTY=4.196497
PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG
PRIMER_INTERNAL_29=36,23
PRIMER_INTERNAL_29_TM=58.804
PRIMER_INTERNAL_29_GC_PERCENT=56.522
PRIMER_INTERNAL_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_30_PENALTY=4.232604
PRIMER_INTERNAL_30_SEQUENCE=GCCATTCGATCCCGACCCAAATCG
PRIMER_INTERNAL_30=68,24
PRIMER_INTERNAL_30_TM=60.233
PRIMER_INTERNAL_30_GC_PERCENT=58.333
PRIMER_INTERNAL_30_SELF_ANY_TH=0.00
PRIMER_INTERNAL_30_SELF_END_TH=0.00
PRIMER_INTERNAL_30_HAIRPIN_TH=41.60
PRIMER_INTERNAL_31_PENALTY=4.351864
PRIMER_INTERNAL_31_SEQUENCE=TTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_31=61,24
PRIMER_INTERNAL_31_TM=60.352
PRIMER_INTERNAL_31_GC_PERCENT=58.333
PRIMER_INTERNAL_31_SELF_ANY_TH=7.03
PRIMER_INTERNAL_31_SELF_END_TH=0.00
PRIMER_INTERNAL_31_HAIRPIN_TH=41.60
PRIMER_INTERNAL_32_PENALTY=4.478673
PRIMER_INTERNAL_32_SEQUENCE=TTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_32=61,23
PRIMER_INTERNAL_32_TM=58.521
PRIMER_INTERNAL_32_GC_PERCENT=56.522
PRIMER_INTERNAL_32_SELF_ANY_TH=7.03
PRIMER_INTERNAL_32_SELF_END_TH=0.00
PRIMER_INTERNAL_32_HAIRPIN_TH=41.60
PRIMER_INTERNAL_33_PENALTY=4.484274
PRIMER_INTERNAL_33_SEQUENCE=TTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_33=61,22
PRIMER_INTERNAL_33_TM=57.516
PRIMER_INTERNAL_33_GC_PERCENT=54.545
PRIMER_INTERNAL_33_SELF_ANY_TH=7.03
PRIMER_INTERNAL_33_SELF_END_TH=0.00
PRIMER_INTERNAL_33_HAIRPIN_TH=39.49
PRIMER_INTERNAL_34_PENALTY=4.552303
PRIMER_INTERNAL_34_SEQUENCE=ACACACACACACACAGCACACA
PRIMER_INTERNAL_34=35,22
PRIMER_INTERNAL_34_TM=57.448
PRIMER_INTERNAL_34_GC_PERCENT=50.000
PRIMER_INTERNAL_34_SELF_ANY_TH=0.00
PRIMER_INTERNAL_34_SELF_END_TH=0.00
PRIMER_INTERNAL_34_HAIRPIN_TH=0.00
PRIMER_INTERNAL_35_PENALTY=4.639434
PRIMER_INTERNAL_35_SEQUENCE=GCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_35=68,22
PRIMER_INTERNAL_35_TM=57.361
PRIMER_INTERNAL_35_GC_PERCENT=54.545
PRIMER_INTERNAL_35_SELF_ANY_TH=0.00
PRIMER_INTERNAL_35_SELF_END_TH=0.00
PRIMER_INTERNAL_35_HAIRPIN_TH=41.60
PRIMER_INTERNAL_36_PENALTY=4.809017
PRIMER_INTERNAL_36_SEQUENCE=ACACACACACACACAGCACACAG
PRIMER_INTERNAL_36=35,23
PRIMER_INTERNAL_36_TM=58.191
PRIMER_INTERNAL_36_GC_PERCENT=52.174
PRIMER_INTERNAL_36_SELF_ANY_TH=0.00
PRIMER_INTERNAL_36_SELF_END_TH=0.00
PRIMER_INTERNAL_36_HAIRPIN_TH=0.00
PRIMER_INTERNAL_37_PENALTY=4.881095
PRIMER_INTERNAL_37_SEQUENCE=GCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_37=68,23
PRIMER_INTERNAL_37_TM=58.119
PRIMER_INTERNAL_37_GC_PERCENT=56.522
PRIMER_INTERNAL_37_SELF_ANY_TH=0.00
PRIMER_INTERNAL_37_SELF_END_TH=0.00
PRIMER_INTERNAL_37_HAIRPIN_TH=41.60
PRIMER_INTERNAL_38_PENALTY=5.108025
PRIMER_INTERNAL_38_SEQUENCE=TGGGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_38=65,23
PRIMER_INTERNAL_38_TM=62.108
PRIMER_INTERNAL_38_GC_PERCENT=60.870
PRIMER_INTERNAL_38_SELF_ANY_TH=0.00
PRIMER_INTERNAL_38_SELF_END_TH=0.00
PRIMER_INTERNAL_38_HAIRPIN_TH=41.29
PRIMER_INTERNAL_39_PENALTY=5.111368
PRIMER_INTERNAL_39_SEQUENCE=CCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_39=69,24
PRIMER_INTERNAL_39_TM=58.889
PRIMER_INTERNAL_39_GC_PERCENT=54.167
PRIMER_INTERNAL_39_SELF_ANY_TH=0.00
PRIMER_INTERNAL_39_SELF_END_TH=0.00
PRIMER_INTERNAL_39_HAIRPIN_TH=42.01
PRIMER_INTERNAL_40_PENALTY=5.111455
PRIMER_INTERNAL_40_SEQUENCE=CCATTCGATCCCGACCCAAATCG
PRIMER_INTERNAL_40=69,23
PRIMER_INTERNAL_40_TM=57.889
PRIMER_INTERNAL_40_GC_PERCENT=56.522
PRIMER_INTERNAL_40_SELF_ANY_TH=0.00
PRIMER_INTERNAL_40_SELF_END_TH=0.00
PRIMER_INTERNAL_40_HAIRPIN_TH=41.60
PRIMER_INTERNAL_41_PENALTY=5.122599
PRIMER_INTERNAL_41_SEQUENCE=GGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_41=66,24
PRIMER_INTERNAL_41_TM=61.123
PRIMER_INTERNAL_41_GC_PERCENT=58.333
PRIMER_INTERNAL_41_SELF_ANY_TH=2.18
PRIMER_INTERNAL_41_SELF_END_TH=0.00
PRIMER_INTERNAL_41_HAIRPIN_TH=41.60
PRIMER_INTERNAL_42_PENALTY=5.295331
PRIMER_INTERNAL_42_SEQUENCE=ACACACACACACAGCACACAGGTA
PRIMER_INTERNAL_42=37,24
PRIMER_INTERNAL_42_TM=58.705
PRIMER_INTERNAL_42_GC_PERCENT=50.000
PRIMER_INTERNAL_42_SELF_ANY_TH=0.00
PRIMER_INTERNAL_42_SELF_END_TH=0.00
PRIMER_INTERNAL_42_HAIRPIN_TH=0.00
PRIMER_INTERNAL_43_PENALTY=5.316271
PRIMER_INTERNAL_43_SEQUENCE=ATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_43=60,23
PRIMER_INTERNAL_43_TM=57.684
PRIMER_INTERNAL_43_GC_PERCENT=52.174
PRIMER_INTERNAL_43_SELF_ANY_TH=7.03
PRIMER_INTERNAL_43_SELF_END_TH=0.00
PRIMER_INTERNAL_43_HAIRPIN_TH=39.49
PRIMER_INTERNAL_44_PENALTY=5.357946
PRIMER_INTERNAL_44_SEQUENCE=ATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_44=60,24
PRIMER_INTERNAL_44_TM=58.642
PRIMER_INTERNAL_44_GC_PERCENT=54.167
PRIMER_INTERNAL_44_SELF_ANY_TH=7.03
PRIMER_INTERNAL_44_SELF_END_TH=0.00
PRIMER_INTERNAL_44_HAIRPIN_TH=41.60
PRIMER_INTERNAL_45_PENALTY=5.401146
PRIMER_INTERNAL_45_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_45=60,25
PRIMER_INTERNAL_45_TM=60.401
PRIMER_INTERNAL_45_GC_PERCENT=56.000
PRIMER_INTERNAL_45_SELF_ANY_TH=7.03
PRIMER_INTERNAL_45_SELF_END_TH=0.00
PRIMER_INTERNAL_45_HAIRPIN_TH=41.60
PRIMER_INTERNAL_46_PENALTY=5.439697
PRIMER_INTERNAL_46_SEQUENCE=CACACACACACACAGCACACAGGTA
PRIMER_INTERNAL_46=36,25
PRIMER_INTERNAL_46_TM=59.560
PRIMER_INTERNAL_46_GC_PERCENT=52.000
PRIMER_INTERNAL_46_SELF_ANY_TH=0.00
PRIMER_INTERNAL_46_SELF_END_TH=0.00
PRIMER_INTERNAL_46_HAIRPIN_TH=0.00
PRIMER_INTERNAL_47_PENALTY=5.459011
PRIMER_INTERNAL_47_SEQUENCE=AGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_47=63,23
PRIMER_INTERNAL_47_TM=62.459
PRIMER_INTERNAL_47_GC_PERCENT=65.217
PRIMER_INTERNAL_47_SELF_ANY_TH=7.03
PRIMER_INTERNAL_47_SELF_END_TH=0.00
PRIMER_INTERNAL_47_HAIRPIN_TH=41.60
PRIMER_INTERNAL_48_PENALTY=5.514983
PRIMER_INTERNAL_48_SEQUENCE=TCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_48=73,24
PRIMER_INTERNAL_48_TM=58.485
PRIMER_INTERNAL_48_GC_PERCENT=54.167
PRIMER_INTERNAL_48_SELF_ANY_TH=0.00
PRIMER_INTERNAL_48_SELF_END_TH=0.00
PRIMER_INTERNAL_48_HAIRPIN_TH=38.51
PRIMER_INTERNAL_49_PENALTY=5.518553
PRIMER_INTERNAL_49_SEQUENCE=CGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_49=74,24
PRIMER_INTERNAL_49_TM=58.481
PRIMER_INTERNAL_49_GC_PERCENT=54.167
PRIMER_INTERNAL_49_SELF_ANY_TH=0.00
PRIMER_INTERNAL_49_SELF_END_TH=0.00
PRIMER_INTERNAL_49_HAIRPIN_TH=41.28
PRIMER_INTERNAL_50_PENALTY=5.523565
PRIMER_INTERNAL_50_SEQUENCE=CGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_50=74,23
PRIMER_INTERNAL_50_TM=57.476
PRIMER_INTERNAL_50_GC_PERCENT=56.522
PRIMER_INTERNAL_50_SELF_ANY_TH=0.00
PRIMER_INTERNAL_50_SELF_END_TH=0.00
PRIMER_INTERNAL_50_HAIRPIN_TH=41.28
PRIMER_INTERNAL_51_PENALTY=5.550131
PRIMER_INTERNAL_51_SEQUENCE=CACACACACACAGCACACAGGTA
PRIMER_INTERNAL_51=38,23
PRIMER_INTERNAL_51_TM=57.450
PRIMER_INTERNAL_51_GC_PERCENT=52.174
PRIMER_INTERNAL_51_SELF_ANY_TH=0.00
PRIMER_INTERNAL_51_SELF_END_TH=0.00
PRIMER_INTERNAL_51_HAIRPIN_TH=0.00
PRIMER_INTERNAL_52_PENALTY=5.564155
PRIMER_INTERNAL_52_SEQUENCE=TCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_52=73,25
PRIMER_INTERNAL_52_TM=59.436
PRIMER_INTERNAL_52_GC_PERCENT=52.000
PRIMER_INTERNAL_52_SELF_ANY_TH=0.00
PRIMER_INTERNAL_52_SELF_END_TH=0.00
PRIMER_INTERNAL_52_HAIRPIN_TH=38.51
PRIMER_INTERNAL_53_PENALTY=5.697915
PRIMER_INTERNAL_53_SEQUENCE=GTGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_53=64,23
PRIMER_INTERNAL_53_TM=62.698
PRIMER_INTERNAL_53_GC_PERCENT=65.217
PRIMER_INTERNAL_53_SELF_ANY_TH=7.03
PRIMER_INTERNAL_53_SELF_END_TH=3.99
PRIMER_INTERNAL_53_HAIRPIN_TH=42.67
PRIMER_INTERNAL_54_PENALTY=5.806699
PRIMER_INTERNAL_54_SEQUENCE=CCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_54=78,24
PRIMER_INTERNAL_54_TM=58.193
PRIMER_INTERNAL_54_GC_PERCENT=54.167
PRIMER_INTERNAL_54_SELF_ANY_TH=12.68
PRIMER_INTERNAL_54_SELF_END_TH=12.68
PRIMER_INTERNAL_54_HAIRPIN_TH=41.28
PRIMER_INTERNAL_55_PENALTY=5.806699
PRIMER_INTERNAL_55_SEQUENCE=TCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_55=77,24
PRIMER_INTERNAL_55_TM=58.193
PRIMER_INTERNAL_55_GC_PERCENT=54.167
PRIMER_INTERNAL_55_SELF_ANY_TH=0.00
PRIMER_INTERNAL_55_SELF_END_TH=0.00
PRIMER_INTERNAL_55_HAIRPIN_TH=41.28
PRIMER_INTERNAL_56_PENALTY=5.824076
PRIMER_INTERNAL_56_SEQUENCE=CCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_56=78,23
PRIMER_INTERNAL_56_TM=57.176
PRIMER_INTERNAL_56_GC_PERCENT=56.522
PRIMER_INTERNAL_56_SELF_ANY_TH=0.00
PRIMER_INTERNAL_56_SELF_END_TH=0.00
PRIMER_INTERNAL_56_HAIRPIN_TH=41.28
PRIMER_INTERNAL_57_PENALTY=5.846052
PRIMER_INTERNAL_57_SEQUENCE=TCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_57=77,25
PRIMER_INTERNAL_57_TM=59.154
PRIMER_INTERNAL_57_GC_PERCENT=52.000
PRIMER_INTERNAL_57_SELF_ANY_TH=12.68
PRIMER_INTERNAL_57_SELF_END_TH=12.68
PRIMER_INTERNAL_57_HAIRPIN_TH=41.28
PRIMER_INTERNAL_58_PENALTY=5.892980
PRIMER_INTERNAL_58_SEQUENCE=TAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_58=62,24
PRIMER_INTERNAL_58_TM=61.893
PRIMER_INTERNAL_58_GC_PERCENT=62.500
PRIMER_INTERNAL_58_SELF_ANY_TH=7.03
PRIMER_INTERNAL_58_SELF_END_TH=0.00
PRIMER_INTERNAL_58_HAIRPIN_TH=41.60
PRIMER_INTERNAL_59_PENALTY=5.951461
PRIMER_INTERNAL_59_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_59=57,25
PRIMER_INTERNAL_59_TM=59.049
PRIMER_INTERNAL_59_GC_PERCENT=56.000
PRIMER_INTERNAL_59_SELF_ANY_TH=7.03
PRIMER_INTERNAL_59_SELF_END_TH=0.00
PRIMER_INTERNAL_59_HAIRPIN_TH=39.49
PRIMER_INTERNAL_60_PENALTY=5.980536
PRIMER_INTERNAL_60_SEQUENCE=CATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_60=70,23
PRIMER_INTERNAL_60_TM=57.019
PRIMER_INTERNAL_60_GC_PERCENT=52.174
PRIMER_INTERNAL_60_SELF_ANY_TH=0.00
PRIMER_INTERNAL_60_SELF_END_TH=0.00
PRIMER_INTERNAL_60_HAIRPIN_TH=42.01
PRIMER_INTERNAL_61_PENALTY=6.009338
PRIMER_INTERNAL_61_SEQUENCE=CCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_61=69,25
PRIMER_INTERNAL_61_TM=58.991
PRIMER_INTERNAL_61_GC_PERCENT=52.000
PRIMER_INTERNAL_61_SELF_ANY_TH=2.97
PRIMER_INTERNAL_61_SELF_END_TH=0.00
PRIMER_INTERNAL_61_HAIRPIN_TH=41.68
PRIMER_INTERNAL_62_PENALTY=6.046206
PRIMER_INTERNAL_62_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_62=59,26
PRIMER_INTERNAL_62_TM=59.954
PRIMER_INTERNAL_62_GC_PERCENT=53.846
PRIMER_INTERNAL_62_SELF_ANY_TH=7.03
PRIMER_INTERNAL_62_SELF_END_TH=0.00
PRIMER_INTERNAL_62_HAIRPIN_TH=41.60
PRIMER_INTERNAL_63_PENALTY=6.046206
PRIMER_INTERNAL_63_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_63=57,26
PRIMER_INTERNAL_63_TM=59.954
PRIMER_INTERNAL_63_GC_PERCENT=53.846
PRIMER_INTERNAL_63_SELF_ANY_TH=7.03
PRIMER_INTERNAL_63_SELF_END_TH=0.00
PRIMER_INTERNAL_63_HAIRPIN_TH=39.49
PRIMER_INTERNAL_64_PENALTY=6.046659
PRIMER_INTERNAL_64_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_64=56,26
PRIMER_INTERNAL_64_TM=59.953
PRIMER_INTERNAL_64_GC_PERCENT=53.846
PRIMER_INTERNAL_64_SELF_ANY_TH=7.03
PRIMER_INTERNAL_64_SELF_END_TH=0.00
PRIMER_INTERNAL_64_HAIRPIN_TH=39.49
PRIMER_INTERNAL_65_PENALTY=6.125318
PRIMER_INTERNAL_65_SEQUENCE=GCCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_65=68,25
PRIMER_INTERNAL_65_TM=61.125
PRIMER_INTERNAL_65_GC_PERCENT=56.000
PRIMER_INTERNAL_65_SELF_ANY_TH=0.00
PRIMER_INTERNAL_65_SELF_END_TH=0.00
PRIMER_INTERNAL_65_HAIRPIN_TH=42.01
PRIMER_INTERNAL_66_PENALTY=6.131527
PRIMER_INTERNAL_66_SEQUENCE=ACACACACACACACAGCACACAGGT
PRIMER_INTERNAL_66=35,25
PRIMER_INTERNAL_66_TM=61.132
PRIMER_INTERNAL_66_GC_PERCENT=52.000
PRIMER_INTERNAL_66_SELF_ANY_TH=0.00
PRIMER_INTERNAL_66_SELF_END_TH=0.00
PRIMER_INTERNAL_66_HAIRPIN_TH=0.00
PRIMER_INTERNAL_67_PENALTY=6.186005
PRIMER_INTERNAL_67_SEQUENCE=ACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_67=37,25
PRIMER_INTERNAL_67_TM=58.814
PRIMER_INTERNAL_67_GC_PERCENT=48.000
PRIMER_INTERNAL_67_SELF_ANY_TH=0.00
PRIMER_INTERNAL_67_SELF_END_TH=0.00
PRIMER_INTERNAL_67_HAIRPIN_TH=0.00
PRIMER_INTERNAL_68_PENALTY=6.225896
PRIMER_INTERNAL_68_SEQUENCE=TTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_68=72,25
PRIMER_INTERNAL_68_TM=58.774
PRIMER_INTERNAL_68_GC_PERCENT=52.000
PRIMER_INTERNAL_68_SELF_ANY_TH=0.00
PRIMER_INTERNAL_68_SELF_END_TH=0.00
PRIMER_INTERNAL_68_HAIRPIN_TH=41.68
PRIMER_INTERNAL_69_PENALTY=6.265114
PRIMER_INTERNAL_69_SEQUENCE=TGGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_69=65,24
PRIMER_INTERNAL_69_TM=62.265
PRIMER_INTERNAL_69_GC_PERCENT=58.333
PRIMER_INTERNAL_69_SELF_ANY_TH=0.00
PRIMER_INTERNAL_69_SELF_END_TH=0.00
PRIMER_INTERNAL_69_HAIRPIN_TH=41.29
PRIMER_INTERNAL_70_PENALTY=6.318558
PRIMER_INTERNAL_70_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_70=72,26
PRIMER_INTERNAL_70_TM=59.681
PRIMER_INTERNAL_70_GC_PERCENT=50.000
PRIMER_INTERNAL_70_SELF_ANY_TH=0.00
PRIMER_INTERNAL_70_SELF_END_TH=0.00
PRIMER_INTERNAL_70_HAIRPIN_TH=41.68
PRIMER_INTERNAL_71_PENALTY=6.365515
PRIMER_INTERNAL_71_SEQUENCE=CACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_71=36,26
PRIMER_INTERNAL_71_TM=59.634
PRIMER_INTERNAL_71_GC_PERCENT=50.000
PRIMER_INTERNAL_71_SELF_ANY_TH=0.00
PRIMER_INTERNAL_71_SELF_END_TH=0.00
PRIMER_INTERNAL_71_HAIRPIN_TH=0.00
PRIMER_INTERNAL_72_PENALTY=6.390437
PRIMER_INTERNAL_72_SEQUENCE=CACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_72=38,24
PRIMER_INTERNAL_72_TM=57.610
PRIMER_INTERNAL_72_GC_PERCENT=50.000
PRIMER_INTERNAL_72_SELF_ANY_TH=0.00
PRIMER_INTERNAL_72_SELF_END_TH=0.00
PRIMER_INTERNAL_72_HAIRPIN_TH=0.00
PRIMER_INTERNAL_73_PENALTY=6.635581
PRIMER_INTERNAL_73_SEQUENCE=GGGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_73=66,25
PRIMER_INTERNAL_73_TM=61.636
PRIMER_INTERNAL_73_GC_PERCENT=60.000
PRIMER_INTERNAL_73_SELF_ANY_TH=2.18
PRIMER_INTERNAL_73_SELF_END_TH=0.00
PRIMER_INTERNAL_73_HAIRPIN_TH=41.60
PRIMER_INTERNAL_74_PENALTY=6.661393
PRIMER_INTERNAL_74_SEQUENCE=ACACACACACACACAGCACACAGGTA
PRIMER_INTERNAL_74=35,26
PRIMER_INTERNAL_74_TM=60.661
PRIMER_INTERNAL_74_GC_PERCENT=50.000
PRIMER_INTERNAL_74_SELF_ANY_TH=0.00
PRIMER_INTERNAL_74_SELF_END_TH=0.00
PRIMER_INTERNAL_74_HAIRPIN_TH=0.00
PRIMER_INTERNAL_75_PENALTY=6.679783
PRIMER_INTERNAL_75_SEQUENCE=CCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_75=78,25
PRIMER_INTERNAL_75_TM=58.320
PRIMER_INTERNAL_75_GC_PERCENT=52.000
PRIMER_INTERNAL_75_SELF_ANY_TH=14.48
PRIMER_INTERNAL_75_SELF_END_TH=14.48
PRIMER_INTERNAL_75_HAIRPIN_TH=46.54
PRIMER_INTERNAL_76_PENALTY=6.679783
PRIMER_INTERNAL_76_SEQUENCE=ATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_76=76,25
PRIMER_INTERNAL_76_TM=58.320
PRIMER_INTERNAL_76_GC_PERCENT=52.000
PRIMER_INTERNAL_76_SELF_ANY_TH=0.00
PRIMER_INTERNAL_76_SELF_END_TH=0.00
PRIMER_INTERNAL_76_HAIRPIN_TH=41.28
PRIMER_INTERNAL_77_PENALTY=6.695664
PRIMER_INTERNAL_77_SEQUENCE=TATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_77=59,24
PRIMER_INTERNAL_77_TM=57.304
PRIMER_INTERNAL_77_GC_PERCENT=50.000
PRIMER_INTERNAL_77_SELF_ANY_TH=7.03
PRIMER_INTERNAL_77_SELF_END_TH=0.00
PRIMER_INTERNAL_77_HAIRPIN_TH=39.49
PRIMER_INTERNAL_78_PENALTY=6.741797
PRIMER_INTERNAL_78_SEQUENCE=GTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_78=58,24
PRIMER_INTERNAL_78_TM=57.258
PRIMER_INTERNAL_78_GC_PERCENT=54.167
PRIMER_INTERNAL_78_SELF_ANY_TH=7.03
PRIMER_INTERNAL_78_SELF_END_TH=0.00
PRIMER_INTERNAL_78_HAIRPIN_TH=39.49
PRIMER_INTERNAL_79_PENALTY=6.754240
PRIMER_INTERNAL_79_SEQUENCE=TATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_79=59,25
PRIMER_INTERNAL_79_TM=58.246
PRIMER_INTERNAL_79_GC_PERCENT=52.000
PRIMER_INTERNAL_79_SELF_ANY_TH=7.03
PRIMER_INTERNAL_79_SELF_END_TH=0.00
PRIMER_INTERNAL_79_HAIRPIN_TH=41.60
PRIMER_INTERNAL_80_PENALTY=6.754240
PRIMER_INTERNAL_80_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_80=58,25
PRIMER_INTERNAL_80_TM=58.246
PRIMER_INTERNAL_80_GC_PERCENT=52.000
PRIMER_INTERNAL_80_SELF_ANY_TH=7.03
PRIMER_INTERNAL_80_SELF_END_TH=0.00
PRIMER_INTERNAL_80_HAIRPIN_TH=39.49
PRIMER_INTERNAL_81_PENALTY=6.756422
PRIMER_INTERNAL_81_SEQUENCE=TCCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_81=77,26
PRIMER_INTERNAL_81_TM=59.244
PRIMER_INTERNAL_81_GC_PERCENT=50.000
PRIMER_INTERNAL_81_SELF_ANY_TH=14.48
PRIMER_INTERNAL_81_SELF_END_TH=14.48
PRIMER_INTERNAL_81_HAIRPIN_TH=46.54
PRIMER_INTERNAL_82_PENALTY=6.756422
PRIMER_INTERNAL_82_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_82=76,26
PRIMER_INTERNAL_82_TM=59.244
PRIMER_INTERNAL_82_GC_PERCENT=50.000
PRIMER_INTERNAL_82_SELF_ANY_TH=12.68
PRIMER_INTERNAL_82_SELF_END_TH=12.68
PRIMER_INTERNAL_82_HAIRPIN_TH=41.28
PRIMER_INTERNAL_83_PENALTY=6.804314
PRIMER_INTERNAL_83_SEQUENCE=CATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_83=70,24
PRIMER_INTERNAL_83_TM=57.196
PRIMER_INTERNAL_83_GC_PERCENT=50.000
PRIMER_INTERNAL_83_SELF_ANY_TH=2.97
PRIMER_INTERNAL_83_SELF_END_TH=0.00
PRIMER_INTERNAL_83_HAIRPIN_TH=41.68
PRIMER_INTERNAL_84_PENALTY=6.828710
PRIMER_INTERNAL_84_SEQUENCE=GTGGGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_84=64,24
PRIMER_INTERNAL_84_TM=62.829
PRIMER_INTERNAL_84_GC_PERCENT=62.500
PRIMER_INTERNAL_84_SELF_ANY_TH=7.03
PRIMER_INTERNAL_84_SELF_END_TH=0.00
PRIMER_INTERNAL_84_HAIRPIN_TH=43.79
PRIMER_INTERNAL_85_PENALTY=6.841708
PRIMER_INTERNAL_85_SEQUENCE=CCCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_85=78,26
PRIMER_INTERNAL_85_TM=59.158
PRIMER_INTERNAL_85_GC_PERCENT=53.846
PRIMER_INTERNAL_85_SELF_ANY_TH=18.40
PRIMER_INTERNAL_85_SELF_END_TH=11.69
PRIMER_INTERNAL_85_HAIRPIN_TH=43.02
PRIMER_INTERNAL_86_PENALTY=6.895596
PRIMER_INTERNAL_86_SEQUENCE=GGCCATTCGATCCCGACCCAAATCG
PRIMER_INTERNAL_86=67,25
PRIMER_INTERNAL_86_TM=61.896
PRIMER_INTERNAL_86_GC_PERCENT=60.000
PRIMER_INTERNAL_86_SELF_ANY_TH=0.00
PRIMER_INTERNAL_86_SELF_END_TH=0.00
PRIMER_INTERNAL_86_HAIRPIN_TH=41.60
PRIMER_INTERNAL_87_PENALTY=6.905174
PRIMER_INTERNAL_87_SEQUENCE=CGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_87=74,25
PRIMER_INTERNAL_87_TM=58.095
PRIMER_INTERNAL_87_GC_PERCENT=52.000
PRIMER_INTERNAL_87_SELF_ANY_TH=0.00
PRIMER_INTERNAL_87_SELF_END_TH=0.00
PRIMER_INTERNAL_87_HAIRPIN_TH=41.28
PRIMER_INTERNAL_88_PENALTY=6.905408
PRIMER_INTERNAL_88_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_88=58,26
PRIMER_INTERNAL_88_TM=59.095
PRIMER_INTERNAL_88_GC_PERCENT=53.846
PRIMER_INTERNAL_88_SELF_ANY_TH=7.03
PRIMER_INTERNAL_88_SELF_END_TH=0.00
PRIMER_INTERNAL_88_HAIRPIN_TH=41.60
PRIMER_INTERNAL_89_PENALTY=6.917157
PRIMER_INTERNAL_89_SEQUENCE=ACACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_89=37,26
PRIMER_INTERNAL_89_TM=59.083
PRIMER_INTERNAL_89_GC_PERCENT=46.154
PRIMER_INTERNAL_89_SELF_ANY_TH=0.00
PRIMER_INTERNAL_89_SELF_END_TH=0.00
PRIMER_INTERNAL_89_HAIRPIN_TH=0.00
PRIMER_INTERNAL_90_PENALTY=6.956454
PRIMER_INTERNAL_90_SEQUENCE=AGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_90=10,24
PRIMER_INTERNAL_90_TM=57.044
PRIMER_INTERNAL_90_GC_PERCENT=50.000
PRIMER_INTERNAL_90_SELF_ANY_TH=0.00
PRIMER_INTERNAL_90_SELF_END_TH=0.00
PRIMER_INTERNAL_90_HAIRPIN_TH=36.06
PRIMER_INTERNAL_91_PENALTY=6.975446
PRIMER_INTERNAL_91_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_91=73,26
PRIMER_INTERNAL_91_TM=59.025
PRIMER_INTERNAL_91_GC_PERCENT=50.000
PRIMER_INTERNAL_91_SELF_ANY_TH=0.00
PRIMER_INTERNAL_91_SELF_END_TH=0.00
PRIMER_INTERNAL_91_HAIRPIN_TH=38.51
PRIMER_INTERNAL_92_PENALTY=7.015932
PRIMER_INTERNAL_92_SEQUENCE=CCCGACCCAAATCGATAGCTACGATGA
PRIMER_INTERNAL_92=78,27
PRIMER_INTERNAL_92_TM=60.016
PRIMER_INTERNAL_92_GC_PERCENT=51.852
PRIMER_INTERNAL_92_SELF_ANY_TH=18.40
PRIMER_INTERNAL_92_SELF_END_TH=5.09
PRIMER_INTERNAL_92_HAIRPIN_TH=43.02
PRIMER_INTERNAL_93_PENALTY=7.015932
PRIMER_INTERNAL_93_SEQUENCE=TCCCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_93=77,27
PRIMER_INTERNAL_93_TM=60.016
PRIMER_INTERNAL_93_GC_PERCENT=51.852
PRIMER_INTERNAL_93_SELF_ANY_TH=18.40
PRIMER_INTERNAL_93_SELF_END_TH=11.69
PRIMER_INTERNAL_93_HAIRPIN_TH=43.02
PRIMER_INTERNAL_94_PENALTY=7.051167
PRIMER_INTERNAL_94_SEQUENCE=TTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_94=61,25
PRIMER_INTERNAL_94_TM=62.051
PRIMER_INTERNAL_94_GC_PERCENT=60.000
PRIMER_INTERNAL_94_SELF_ANY_TH=7.03
PRIMER_INTERNAL_94_SELF_END_TH=0.00
PRIMER_INTERNAL_94_HAIRPIN_TH=41.60
PRIMER_INTERNAL_95_PENALTY=7.056335
PRIMER_INTERNAL_95_SEQUENCE=GATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_95=75,26
PRIMER_INTERNAL_95_TM=58.944
PRIMER_INTERNAL_95_GC_PERCENT=53.846
PRIMER_INTERNAL_95_SELF_ANY_TH=0.00
PRIMER_INTERNAL_95_SELF_END_TH=0.00
PRIMER_INTERNAL_95_HAIRPIN_TH=41.28
PRIMER_INTERNAL_96_PENALTY=7.056335
PRIMER_INTERNAL_96_SEQUENCE=CGATCCCGACCCAAATCGATAGCTAC
PRIMER_INTERNAL_96=74,26
PRIMER_INTERNAL_96_TM=58.944
PRIMER_INTERNAL_96_GC_PERCENT=53.846
PRIMER_INTERNAL_96_SELF_ANY_TH=0.00
PRIMER_INTERNAL_96_SELF_END_TH=0.00
PRIMER_INTERNAL_96_HAIRPIN_TH=41.28
PRIMER_INTERNAL_97_PENALTY=7.066919
PRIMER_INTERNAL_97_SEQUENCE=CACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_97=38,25
PRIMER_INTERNAL_97_TM=57.933
PRIMER_INTERNAL_97_GC_PERCENT=48.000
PRIMER_INTERNAL_97_SELF_ANY_TH=0.00
PRIMER_INTERNAL_97_SELF_END_TH=0.00
PRIMER_INTERNAL_97_HAIRPIN_TH=0.00
PRIMER_INTERNAL_98_PENALTY=7.070364
PRIMER_INTERNAL_98_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_98=56,25
PRIMER_INTERNAL_98_TM=57.930
PRIMER_INTERNAL_98_GC_PERCENT=52.000
PRIMER_INTERNAL_98_SELF_ANY_TH=7.03
PRIMER_INTERNAL_98_SELF_END_TH=0.00
PRIMER_INTERNAL_98_HAIRPIN_TH=38.81
PRIMER_INTERNAL_99_PENALTY=7.094172
PRIMER_INTERNAL_99_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_99=54,27
PRIMER_INTERNAL_99_TM=59.906
PRIMER_INTERNAL_99_GC_PERCENT=51.852
PRIMER_INTERNAL_99_SELF_ANY_TH=7.03
PRIMER_INTERNAL_99_SELF_END_TH=0.00
PRIMER_INTERNAL_99_HAIRPIN_TH=38.81
PRIMER_INTERNAL_100_PENALTY=7.124042
PRIMER_INTERNAL_100_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_100=71,26
PRIMER_INTERNAL_100_TM=58.876
PRIMER_INTERNAL_100_GC_PERCENT=50.000
PRIMER_INTERNAL_100_SELF_ANY_TH=0.00
PRIMER_INTERNAL_100_SELF_END_TH=0.00
PRIMER_INTERNAL_100_HAIRPIN_TH=41.68
PRIMER_INTERNAL_101_PENALTY=7.139284
PRIMER_INTERNAL_101_SEQUENCE=CACACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_101=36,27
PRIMER_INTERNAL_101_TM=59.861
PRIMER_INTERNAL_101_GC_PERCENT=48.148
PRIMER_INTERNAL_101_SELF_ANY_TH=0.00
PRIMER_INTERNAL_101_SELF_END_TH=0.00
PRIMER_INTERNAL_101_HAIRPIN_TH=0.00
PRIMER_INTERNAL_102_PENALTY=7.145199
PRIMER_INTERNAL_102_SEQUENCE=GCCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_102=68,26
PRIMER_INTERNAL_102_TM=61.145
PRIMER_INTERNAL_102_GC_PERCENT=53.846
PRIMER_INTERNAL_102_SELF_ANY_TH=2.97
PRIMER_INTERNAL_102_SELF_END_TH=0.00
PRIMER_INTERNAL_102_HAIRPIN_TH=41.68
PRIMER_INTERNAL_103_PENALTY=7.192525
PRIMER_INTERNAL_103_SEQUENCE=GATCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_103=75,27
PRIMER_INTERNAL_103_TM=59.807
PRIMER_INTERNAL_103_GC_PERCENT=51.852
PRIMER_INTERNAL_103_SELF_ANY_TH=12.68
PRIMER_INTERNAL_103_SELF_END_TH=12.68
PRIMER_INTERNAL_103_HAIRPIN_TH=41.28
PRIMER_INTERNAL_104_PENALTY=7.192525
PRIMER_INTERNAL_104_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTAC
PRIMER_INTERNAL_104=73,27
PRIMER_INTERNAL_104_TM=59.807
PRIMER_INTERNAL_104_GC_PERCENT=51.852
PRIMER_INTERNAL_104_SELF_ANY_TH=0.00
PRIMER_INTERNAL_104_SELF_END_TH=0.00
PRIMER_INTERNAL_104_HAIRPIN_TH=38.51
PRIMER_INTERNAL_105_PENALTY=7.193259
PRIMER_INTERNAL_105_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_105=55,26
PRIMER_INTERNAL_105_TM=58.807
PRIMER_INTERNAL_105_GC_PERCENT=53.846
PRIMER_INTERNAL_105_SELF_ANY_TH=7.03
PRIMER_INTERNAL_105_SELF_END_TH=0.00
PRIMER_INTERNAL_105_HAIRPIN_TH=38.81
PRIMER_INTERNAL_106_PENALTY=7.239217
PRIMER_INTERNAL_106_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGA
PRIMER_INTERNAL_106=52,25
PRIMER_INTERNAL_106_TM=57.761
PRIMER_INTERNAL_106_GC_PERCENT=48.000
PRIMER_INTERNAL_106_SELF_ANY_TH=7.03
PRIMER_INTERNAL_106_SELF_END_TH=0.00
PRIMER_INTERNAL_106_HAIRPIN_TH=36.70
PRIMER_INTERNAL_107_PENALTY=7.243997
PRIMER_INTERNAL_107_SEQUENCE=TGGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_107=65,25
PRIMER_INTERNAL_107_TM=62.244
PRIMER_INTERNAL_107_GC_PERCENT=56.000
PRIMER_INTERNAL_107_SELF_ANY_TH=0.00
PRIMER_INTERNAL_107_SELF_END_TH=0.00
PRIMER_INTERNAL_107_HAIRPIN_TH=41.29
PRIMER_INTERNAL_108_PENALTY=7.246964
PRIMER_INTERNAL_108_SEQUENCE=ACACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_108=43,26
PRIMER_INTERNAL_108_TM=58.753
PRIMER_INTERNAL_108_GC_PERCENT=50.000
PRIMER_INTERNAL_108_SELF_ANY_TH=0.00
PRIMER_INTERNAL_108_SELF_END_TH=0.00
PRIMER_INTERNAL_108_HAIRPIN_TH=38.74
PRIMER_INTERNAL_109_PENALTY=7.250024
PRIMER_INTERNAL_109_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_109=71,27
PRIMER_INTERNAL_109_TM=59.750
PRIMER_INTERNAL_109_GC_PERCENT=48.148
PRIMER_INTERNAL_109_SELF_ANY_TH=0.00
PRIMER_INTERNAL_109_SELF_END_TH=0.00
PRIMER_INTERNAL_109_HAIRPIN_TH=41.68
PRIMER_INTERNAL_110_PENALTY=7.293083
PRIMER_INTERNAL_110_SEQUENCE=CACACAGGTATTAGTGGGCCATTCG
PRIMER_INTERNAL_110=51,25
PRIMER_INTERNAL_110_TM=57.707
PRIMER_INTERNAL_110_GC_PERCENT=52.000
PRIMER_INTERNAL_110_SELF_ANY_TH=7.03
PRIMER_INTERNAL_110_SELF_END_TH=0.00
PRIMER_INTERNAL_110_HAIRPIN_TH=36.70
PRIMER_INTERNAL_111_PENALTY=7.342607
PRIMER_INTERNAL_111_SEQUENCE=CATTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_111=70,27
PRIMER_INTERNAL_111_TM=59.657
PRIMER_INTERNAL_111_GC_PERCENT=51.852
PRIMER_INTERNAL_111_SELF_ANY_TH=0.00
PRIMER_INTERNAL_111_SELF_END_TH=0.00
PRIMER_INTERNAL_111_HAIRPIN_TH=41.68
PRIMER_INTERNAL_112_PENALTY=7.354919
PRIMER_INTERNAL_112_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGA
PRIMER_INTERNAL_112=51,26
PRIMER_INTERNAL_112_TM=58.645
PRIMER_INTERNAL_112_GC_PERCENT=50.000
PRIMER_INTERNAL_112_SELF_ANY_TH=7.03
PRIMER_INTERNAL_112_SELF_END_TH=0.00
PRIMER_INTERNAL_112_HAIRPIN_TH=36.70
PRIMER_INTERNAL_113_PENALTY=7.401440
PRIMER_INTERNAL_113_SEQUENCE=CCATTCGATCCCGACCCAAATCGATA
PRIMER_INTERNAL_113=69,26
PRIMER_INTERNAL_113_TM=58.599
PRIMER_INTERNAL_113_GC_PERCENT=50.000
PRIMER_INTERNAL_113_SELF_ANY_TH=0.00
PRIMER_INTERNAL_113_SELF_END_TH=0.00
PRIMER_INTERNAL_113_HAIRPIN_TH=41.68
PRIMER_INTERNAL_114_PENALTY=7.408271
PRIMER_INTERNAL_114_SEQUENCE=CACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_114=44,25
PRIMER_INTERNAL_114_TM=57.592
PRIMER_INTERNAL_114_GC_PERCENT=52.000
PRIMER_INTERNAL_114_SELF_ANY_TH=0.00
PRIMER_INTERNAL_114_SELF_END_TH=0.00
PRIMER_INTERNAL_114_HAIRPIN_TH=38.74
PRIMER_INTERNAL_115_PENALTY=7.451841
PRIMER_INTERNAL_115_SEQUENCE=CACACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_115=42,27
PRIMER_INTERNAL_115_TM=59.548
PRIMER_INTERNAL_115_GC_PERCENT=51.852
PRIMER_INTERNAL_115_SELF_ANY_TH=0.00
PRIMER_INTERNAL_115_SELF_END_TH=0.00
PRIMER_INTERNAL_115_HAIRPIN_TH=38.74
PRIMER_INTERNAL_116_PENALTY=7.534114
PRIMER_INTERNAL_116_SEQUENCE=CCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_116=79,25
PRIMER_INTERNAL_116_TM=57.466
PRIMER_INTERNAL_116_GC_PERCENT=52.000
PRIMER_INTERNAL_116_SELF_ANY_TH=18.40
PRIMER_INTERNAL_116_SELF_END_TH=11.69
PRIMER_INTERNAL_116_HAIRPIN_TH=43.02
PRIMER_INTERNAL_117_PENALTY=7.537719
PRIMER_INTERNAL_117_SEQUENCE=CGACCCAAATCGATAGCTACGATGACG
PRIMER_INTERNAL_117=80,27
PRIMER_INTERNAL_117_TM=59.462
PRIMER_INTERNAL_117_GC_PERCENT=51.852
PRIMER_INTERNAL_117_SELF_ANY_TH=18.40
PRIMER_INTERNAL_117_SELF_END_TH=13.91
PRIMER_INTERNAL_117_HAIRPIN_TH=43.02
PRIMER_INTERNAL_118_PENALTY=7.602591
PRIMER_INTERNAL_118_SEQUENCE=CCGACCCAAATCGATAGCTACGATGA
PRIMER_INTERNAL_118=79,26
PRIMER_INTERNAL_118_TM=58.397
PRIMER_INTERNAL_118_GC_PERCENT=50.000
PRIMER_INTERNAL_118_SELF_ANY_TH=18.40
PRIMER_INTERNAL_118_SELF_END_TH=5.09
PRIMER_INTERNAL_118_HAIRPIN_TH=43.02
PRIMER_INTERNAL_119_PENALTY=7.672898
PRIMER_INTERNAL_119_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_119=76,27
PRIMER_INTERNAL_119_TM=59.327
PRIMER_INTERNAL_119_GC_PERCENT=48.148
PRIMER_INTERNAL_119_SELF_ANY_TH=14.48
PRIMER_INTERNAL_119_SELF_END_TH=14.48
PRIMER_INTERNAL_119_HAIRPIN_TH=46.54
PRIMER_INTERNAL_120_PENALTY=7.693260
PRIMER_INTERNAL_120_SEQUENCE=GCCATTCGATCCCGACCCAAATCGATA
PRIMER_INTERNAL_120=68,27
PRIMER_INTERNAL_120_TM=60.693
PRIMER_INTERNAL_120_GC_PERCENT=51.852
PRIMER_INTERNAL_120_SELF_ANY_TH=0.00
PRIMER_INTERNAL_120_SELF_END_TH=0.00
PRIMER_INTERNAL_120_HAIRPIN_TH=41.68
PRIMER_INTERNAL_121_PENALTY=7.693499
PRIMER_INTERNAL_121_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_121=58,27
PRIMER_INTERNAL_121_TM=60.693
PRIMER_INTERNAL_121_GC_PERCENT=55.556
PRIMER_INTERNAL_121_SELF_ANY_TH=7.03
PRIMER_INTERNAL_121_SELF_END_TH=0.00
PRIMER_INTERNAL_121_HAIRPIN_TH=41.60
PRIMER_INTERNAL_122_PENALTY=7.693499
PRIMER_INTERNAL_122_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_122=57,27
PRIMER_INTERNAL_122_TM=60.693
PRIMER_INTERNAL_122_GC_PERCENT=55.556
PRIMER_INTERNAL_122_SELF_ANY_TH=7.03
PRIMER_INTERNAL_122_SELF_END_TH=0.00
PRIMER_INTERNAL_122_HAIRPIN_TH=41.60
PRIMER_INTERNAL_123_PENALTY=7.694218
PRIMER_INTERNAL_123_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_123=55,27
PRIMER_INTERNAL_123_TM=60.694
PRIMER_INTERNAL_123_GC_PERCENT=55.556
PRIMER_INTERNAL_123_SELF_ANY_TH=7.03
PRIMER_INTERNAL_123_SELF_END_TH=0.00
PRIMER_INTERNAL_123_HAIRPIN_TH=39.49
PRIMER_INTERNAL_124_PENALTY=7.696389
PRIMER_INTERNAL_124_SEQUENCE=ACACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_124=35,27
PRIMER_INTERNAL_124_TM=60.696
PRIMER_INTERNAL_124_GC_PERCENT=48.148
PRIMER_INTERNAL_124_SELF_ANY_TH=0.00
PRIMER_INTERNAL_124_SELF_END_TH=0.00
PRIMER_INTERNAL_124_HAIRPIN_TH=0.00
PRIMER_INTERNAL_125_PENALTY=7.723400
PRIMER_INTERNAL_125_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_125=72,27
PRIMER_INTERNAL_125_TM=59.277
PRIMER_INTERNAL_125_GC_PERCENT=48.148
PRIMER_INTERNAL_125_SELF_ANY_TH=0.00
PRIMER_INTERNAL_125_SELF_END_TH=0.00
PRIMER_INTERNAL_125_HAIRPIN_TH=41.68
PRIMER_INTERNAL_126_PENALTY=7.753661
PRIMER_INTERNAL_126_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_126=54,26
PRIMER_INTERNAL_126_TM=58.246
PRIMER_INTERNAL_126_GC_PERCENT=50.000
PRIMER_INTERNAL_126_SELF_ANY_TH=7.03
PRIMER_INTERNAL_126_SELF_END_TH=5.96
PRIMER_INTERNAL_126_HAIRPIN_TH=0.00
PRIMER_INTERNAL_127_PENALTY=7.785822
PRIMER_INTERNAL_127_SEQUENCE=CGATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_127=74,27
PRIMER_INTERNAL_127_TM=60.786
PRIMER_INTERNAL_127_GC_PERCENT=55.556
PRIMER_INTERNAL_127_SELF_ANY_TH=0.00
PRIMER_INTERNAL_127_SELF_END_TH=0.00
PRIMER_INTERNAL_127_HAIRPIN_TH=41.28
PRIMER_INTERNAL_128_PENALTY=7.802259
PRIMER_INTERNAL_128_SEQUENCE=CCGACCCAAATCGATAGCTACGATGAC
PRIMER_INTERNAL_128=79,27
PRIMER_INTERNAL_128_TM=59.198
PRIMER_INTERNAL_128_GC_PERCENT=51.852
PRIMER_INTERNAL_128_SELF_ANY_TH=18.40
PRIMER_INTERNAL_128_SELF_END_TH=9.31
PRIMER_INTERNAL_128_HAIRPIN_TH=43.02
PRIMER_INTERNAL_129_PENALTY=7.812760
PRIMER_INTERNAL_129_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_129=56,27
PRIMER_INTERNAL_129_TM=60.813
PRIMER_INTERNAL_129_GC_PERCENT=51.852
PRIMER_INTERNAL_129_SELF_ANY_TH=7.03
PRIMER_INTERNAL_129_SELF_END_TH=0.00
PRIMER_INTERNAL_129_HAIRPIN_TH=39.49
PRIMER_INTERNAL_130_PENALTY=7.812835
PRIMER_INTERNAL_130_SEQUENCE=CCATTCGATCCCGACCCAAATCGATAG
PRIMER_INTERNAL_130=69,27
PRIMER_INTERNAL_130_TM=59.187
PRIMER_INTERNAL_130_GC_PERCENT=51.852
PRIMER_INTERNAL_130_SELF_ANY_TH=0.00
PRIMER_INTERNAL_130_SELF_END_TH=0.00
PRIMER_INTERNAL_130_HAIRPIN_TH=41.68
PRIMER_INTERNAL_131_PENALTY=7.928092
PRIMER_INTERNAL_131_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_131=53,27
PRIMER_INTERNAL_131_TM=59.072
PRIMER_INTERNAL_131_GC_PERCENT=51.852
PRIMER_INTERNAL_131_SELF_ANY_TH=7.03
PRIMER_INTERNAL_131_SELF_END_TH=5.96
PRIMER_INTERNAL_131_HAIRPIN_TH=29.02
PRIMER_INTERNAL_132_PENALTY=7.938713
PRIMER_INTERNAL_132_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_132=55,25
PRIMER_INTERNAL_132_TM=57.061
PRIMER_INTERNAL_132_GC_PERCENT=52.000
PRIMER_INTERNAL_132_SELF_ANY_TH=7.03
PRIMER_INTERNAL_132_SELF_END_TH=5.96
PRIMER_INTERNAL_132_HAIRPIN_TH=0.00
PRIMER_INTERNAL_133_PENALTY=7.949148
PRIMER_INTERNAL_133_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_133=64,25
PRIMER_INTERNAL_133_TM=62.949
PRIMER_INTERNAL_133_GC_PERCENT=60.000
PRIMER_INTERNAL_133_SELF_ANY_TH=7.03
PRIMER_INTERNAL_133_SELF_END_TH=0.00
PRIMER_INTERNAL_133_HAIRPIN_TH=43.79
PRIMER_INTERNAL_134_PENALTY=7.979746
PRIMER_INTERNAL_134_SEQUENCE=ACACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_134=41,27
PRIMER_INTERNAL_134_TM=59.020
PRIMER_INTERNAL_134_GC_PERCENT=48.148
PRIMER_INTERNAL_134_SELF_ANY_TH=0.00
PRIMER_INTERNAL_134_SELF_END_TH=0.00
PRIMER_INTERNAL_134_HAIRPIN_TH=36.70
PRIMER_INTERNAL_135_PENALTY=7.992102
PRIMER_INTERNAL_135_SEQUENCE=TAGTGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_135=62,25
PRIMER_INTERNAL_135_TM=62.992
PRIMER_INTERNAL_135_GC_PERCENT=60.000
PRIMER_INTERNAL_135_SELF_ANY_TH=7.03
PRIMER_INTERNAL_135_SELF_END_TH=3.99
PRIMER_INTERNAL_135_HAIRPIN_TH=42.67
PRIMER_INTERNAL_136_PENALTY=7.993148
PRIMER_INTERNAL_136_SEQUENCE=ACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_136=43,25
PRIMER_INTERNAL_136_TM=57.007
PRIMER_INTERNAL_136_GC_PERCENT=48.000
PRIMER_INTERNAL_136_SELF_ANY_TH=0.00
PRIMER_INTERNAL_136_SELF_END_TH=0.00
PRIMER_INTERNAL_136_HAIRPIN_TH=36.70
PRIMER_INTERNAL_137_PENALTY=8.038590
PRIMER_INTERNAL_137_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_137=60,26
PRIMER_INTERNAL_137_TM=62.039
PRIMER_INTERNAL_137_GC_PERCENT=57.692
PRIMER_INTERNAL_137_SELF_ANY_TH=7.03
PRIMER_INTERNAL_137_SELF_END_TH=0.00
PRIMER_INTERNAL_137_HAIRPIN_TH=41.60
PRIMER_INTERNAL_138_PENALTY=8.084641
PRIMER_INTERNAL_138_SEQUENCE=CACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_138=42,26
PRIMER_INTERNAL_138_TM=57.915
PRIMER_INTERNAL_138_GC_PERCENT=50.000
PRIMER_INTERNAL_138_SELF_ANY_TH=0.00
PRIMER_INTERNAL_138_SELF_END_TH=0.00
PRIMER_INTERNAL_138_HAIRPIN_TH=36.70
PRIMER_INTERNAL_139_PENALTY=8.098597
PRIMER_INTERNAL_139_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGAT
PRIMER_INTERNAL_139=52,26
PRIMER_INTERNAL_139_TM=57.901
PRIMER_INTERNAL_139_GC_PERCENT=46.154
PRIMER_INTERNAL_139_SELF_ANY_TH=7.03
PRIMER_INTERNAL_139_SELF_END_TH=2.18
PRIMER_INTERNAL_139_HAIRPIN_TH=36.70
PRIMER_INTERNAL_140_PENALTY=8.106799
PRIMER_INTERNAL_140_SEQUENCE=TCAGTAGACATGACACTGACGATGCA
PRIMER_INTERNAL_140=5,26
PRIMER_INTERNAL_140_TM=57.893
PRIMER_INTERNAL_140_GC_PERCENT=46.154
PRIMER_INTERNAL_140_SELF_ANY_TH=13.87
PRIMER_INTERNAL_140_SELF_END_TH=0.00
PRIMER_INTERNAL_140_HAIRPIN_TH=37.56
PRIMER_INTERNAL_141_PENALTY=8.251191
PRIMER_INTERNAL_141_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGAT
PRIMER_INTERNAL_141=51,27
PRIMER_INTERNAL_141_TM=58.749
PRIMER_INTERNAL_141_GC_PERCENT=48.148
PRIMER_INTERNAL_141_SELF_ANY_TH=7.03
PRIMER_INTERNAL_141_SELF_END_TH=2.18
PRIMER_INTERNAL_141_HAIRPIN_TH=36.70
PRIMER_INTERNAL_142_PENALTY=8.275978
PRIMER_INTERNAL_142_SEQUENCE=GTCAGTAGACATGACACTGACGATGCA
PRIMER_INTERNAL_142=4,27
PRIMER_INTERNAL_142_TM=58.724
PRIMER_INTERNAL_142_GC_PERCENT=48.148
PRIMER_INTERNAL_142_SELF_ANY_TH=22.59
PRIMER_INTERNAL_142_SELF_END_TH=0.00
PRIMER_INTERNAL_142_HAIRPIN_TH=46.00
PRIMER_INTERNAL_143_PENALTY=8.300145
PRIMER_INTERNAL_143_SEQUENCE=ACACACACACACAGCACACAGGTATTA
PRIMER_INTERNAL_143=37,27
PRIMER_INTERNAL_143_TM=58.700
PRIMER_INTERNAL_143_GC_PERCENT=44.444
PRIMER_INTERNAL_143_SELF_ANY_TH=0.00
PRIMER_INTERNAL_143_SELF_END_TH=0.00
PRIMER_INTERNAL_143_HAIRPIN_TH=0.00
PRIMER_INTERNAL_144_PENALTY=8.330872
PRIMER_INTERNAL_144_SEQUENCE=AGTAGACATGACACTGACGATGCAGA
PRIMER_INTERNAL_144=7,26
PRIMER_INTERNAL_144_TM=57.669
PRIMER_INTERNAL_144_GC_PERCENT=46.154
PRIMER_INTERNAL_144_SELF_ANY_TH=0.00
PRIMER_INTERNAL_144_SELF_END_TH=0.00
PRIMER_INTERNAL_144_HAIRPIN_TH=36.06
PRIMER_INTERNAL_145_PENALTY=8.376203
PRIMER_INTERNAL_145_SEQUENCE=GTAGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_145=8,26
PRIMER_INTERNAL_145_TM=57.624
PRIMER_INTERNAL_145_GC_PERCENT=50.000
PRIMER_INTERNAL_145_SELF_ANY_TH=0.00
PRIMER_INTERNAL_145_SELF_END_TH=0.00
PRIMER_INTERNAL_145_HAIRPIN_TH=36.06
PRIMER_INTERNAL_146_PENALTY=8.376203
PRIMER_INTERNAL_146_SEQUENCE=GTCAGTAGACATGACACTGACGATGC
PRIMER_INTERNAL_146=4,26
PRIMER_INTERNAL_146_TM=57.624
PRIMER_INTERNAL_146_GC_PERCENT=50.000
PRIMER_INTERNAL_146_SELF_ANY_TH=22.59
PRIMER_INTERNAL_146_SELF_END_TH=10.24
PRIMER_INTERNAL_146_HAIRPIN_TH=46.00
PRIMER_INTERNAL_147_PENALTY=8.379879
PRIMER_INTERNAL_147_SEQUENCE=CAGTAGACATGACACTGACGATGCAG
PRIMER_INTERNAL_147=6,26
PRIMER_INTERNAL_147_TM=57.620
PRIMER_INTERNAL_147_GC_PERCENT=50.000
PRIMER_INTERNAL_147_SELF_ANY_TH=12.06
PRIMER_INTERNAL_147_SELF_END_TH=0.00
PRIMER_INTERNAL_147_HAIRPIN_TH=37.39
PRIMER_INTERNAL_148_PENALTY=8.418488
PRIMER_INTERNAL_148_SEQUENCE=CACACACACACAGCACACAGGTATTA
PRIMER_INTERNAL_148=38,26
PRIMER_INTERNAL_148_TM=57.582
PRIMER_INTERNAL_148_GC_PERCENT=46.154
PRIMER_INTERNAL_148_SELF_ANY_TH=0.00
PRIMER_INTERNAL_148_SELF_END_TH=0.00
PRIMER_INTERNAL_148_HAIRPIN_TH=0.00
PRIMER_INTERNAL_149_PENALTY=8.423914
PRIMER_INTERNAL_149_SEQUENCE=GACCCAAATCGATAGCTACGATGACG
PRIMER_INTERNAL_149=81,26
PRIMER_INTERNAL_149_TM=57.576
PRIMER_INTERNAL_149_GC_PERCENT=50.000
PRIMER_INTERNAL_149_SELF_ANY_TH=18.40
PRIMER_INTERNAL_149_SELF_END_TH=12.20
PRIMER_INTERNAL_149_HAIRPIN_TH=43.02
PRIMER_INTERNAL_150_PENALTY=8.423914
PRIMER_INTERNAL_150_SEQUENCE=CGACCCAAATCGATAGCTACGATGAC
PRIMER_INTERNAL_150=80,26
PRIMER_INTERNAL_150_TM=57.576
PRIMER_INTERNAL_150_GC_PERCENT=50.000
PRIMER_INTERNAL_150_SELF_ANY_TH=18.40
PRIMER_INTERNAL_150_SELF_END_TH=9.31
PRIMER_INTERNAL_150_HAIRPIN_TH=43.02
PRIMER_INTERNAL_151_PENALTY=8.452836
PRIMER_INTERNAL_151_SEQUENCE=CATTCGATCCCGACCCAAATCGATAG
PRIMER_INTERNAL_151=70,26
PRIMER_INTERNAL_151_TM=57.547
PRIMER_INTERNAL_151_GC_PERCENT=50.000
PRIMER_INTERNAL_151_SELF_ANY_TH=0.00
PRIMER_INTERNAL_151_SELF_END_TH=0.00
PRIMER_INTERNAL_151_HAIRPIN_TH=41.68
PRIMER_INTERNAL_152_PENALTY=8.463323
PRIMER_INTERNAL_152_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGATC
PRIMER_INTERNAL_152=52,27
PRIMER_INTERNAL_152_TM=58.537
PRIMER_INTERNAL_152_GC_PERCENT=48.148
PRIMER_INTERNAL_152_SELF_ANY_TH=7.03
PRIMER_INTERNAL_152_SELF_END_TH=0.00
PRIMER_INTERNAL_152_HAIRPIN_TH=36.70
PRIMER_INTERNAL_153_PENALTY=8.486477
PRIMER_INTERNAL_153_SEQUENCE=CAGTAGACATGACACTGACGATGCAGA
PRIMER_INTERNAL_153=6,27
PRIMER_INTERNAL_153_TM=58.514
PRIMER_INTERNAL_153_GC_PERCENT=48.148
PRIMER_INTERNAL_153_SELF_ANY_TH=12.06
PRIMER_INTERNAL_153_SELF_END_TH=0.00
PRIMER_INTERNAL_153_HAIRPIN_TH=37.39
PRIMER_INTERNAL_154_PENALTY=8.486477
PRIMER_INTERNAL_154_SEQUENCE=TCAGTAGACATGACACTGACGATGCAG
PRIMER_INTERNAL_154=5,27
PRIMER_INTERNAL_154_TM=58.514
PRIMER_INTERNAL_154_GC_PERCENT=48.148
PRIMER_INTERNAL_154_SELF_ANY_TH=13.87
PRIMER_INTERNAL_154_SELF_END_TH=0.00
PRIMER_INTERNAL_154_HAIRPIN_TH=37.56
PRIMER_INTERNAL_155_PENALTY=8.487256
PRIMER_INTERNAL_155_SEQUENCE=AGTAGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_155=7,27
PRIMER_INTERNAL_155_TM=58.513
PRIMER_INTERNAL_155_GC_PERCENT=48.148
PRIMER_INTERNAL_155_SELF_ANY_TH=0.00
PRIMER_INTERNAL_155_SELF_END_TH=0.00
PRIMER_INTERNAL_155_HAIRPIN_TH=36.06
PRIMER_INTERNAL_156_PENALTY=8.514997
PRIMER_INTERNAL_156_SEQUENCE=ACACACACACAGCACACAGGTATTAGT
PRIMER_INTERNAL_156=39,27
PRIMER_INTERNAL_156_TM=58.485
PRIMER_INTERNAL_156_GC_PERCENT=44.444
PRIMER_INTERNAL_156_SELF_ANY_TH=0.00
PRIMER_INTERNAL_156_SELF_END_TH=0.00
PRIMER_INTERNAL_156_HAIRPIN_TH=0.00
PRIMER_INTERNAL_157_PENALTY=8.547914
PRIMER_INTERNAL_157_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_157=59,27
PRIMER_INTERNAL_157_TM=61.548
PRIMER_INTERNAL_157_GC_PERCENT=55.556
PRIMER_INTERNAL_157_SELF_ANY_TH=7.03
PRIMER_INTERNAL_157_SELF_END_TH=0.00
PRIMER_INTERNAL_157_HAIRPIN_TH=41.60
PRIMER_INTERNAL_158_PENALTY=8.589885
PRIMER_INTERNAL_158_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATC
PRIMER_INTERNAL_158=53,26
PRIMER_INTERNAL_158_TM=57.410
PRIMER_INTERNAL_158_GC_PERCENT=50.000
PRIMER_INTERNAL_158_SELF_ANY_TH=7.03
PRIMER_INTERNAL_158_SELF_END_TH=0.00
PRIMER_INTERNAL_158_HAIRPIN_TH=29.02
PRIMER_INTERNAL_159_PENALTY=8.642220
PRIMER_INTERNAL_159_SEQUENCE=ACACACACAGCACACAGGTATTAGTG
PRIMER_INTERNAL_159=41,26
PRIMER_INTERNAL_159_TM=57.358
PRIMER_INTERNAL_159_GC_PERCENT=46.154
PRIMER_INTERNAL_159_SELF_ANY_TH=0.00
PRIMER_INTERNAL_159_SELF_END_TH=0.00
PRIMER_INTERNAL_159_HAIRPIN_TH=39.84
PRIMER_INTERNAL_160_PENALTY=8.642220
PRIMER_INTERNAL_160_SEQUENCE=CACACACACAGCACACAGGTATTAGT
PRIMER_INTERNAL_160=40,26
PRIMER_INTERNAL_160_TM=57.358
PRIMER_INTERNAL_160_GC_PERCENT=46.154
PRIMER_INTERNAL_160_SELF_ANY_TH=0.00
PRIMER_INTERNAL_160_SELF_END_TH=0.00
PRIMER_INTERNAL_160_HAIRPIN_TH=0.00
PRIMER_INTERNAL_161_PENALTY=8.642220
PRIMER_INTERNAL_161_SEQUENCE=ACACACACACAGCACACAGGTATTAG
PRIMER_INTERNAL_161=39,26
PRIMER_INTERNAL_161_TM=57.358
PRIMER_INTERNAL_161_GC_PERCENT=46.154
PRIMER_INTERNAL_161_SELF_ANY_TH=0.00
PRIMER_INTERNAL_161_SELF_END_TH=0.00
PRIMER_INTERNAL_161_HAIRPIN_TH=0.00
PRIMER_INTERNAL_162_PENALTY=8.687221
PRIMER_INTERNAL_162_SEQUENCE=TGGGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_162=65,26
PRIMER_INTERNAL_162_TM=62.687
PRIMER_INTERNAL_162_GC_PERCENT=57.692
PRIMER_INTERNAL_162_SELF_ANY_TH=0.00
PRIMER_INTERNAL_162_SELF_END_TH=0.00
PRIMER_INTERNAL_162_HAIRPIN_TH=41.29
PRIMER_INTERNAL_163_PENALTY=8.713667
PRIMER_INTERNAL_163_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_163=67,26
PRIMER_INTERNAL_163_TM=62.714
PRIMER_INTERNAL_163_GC_PERCENT=57.692
PRIMER_INTERNAL_163_SELF_ANY_TH=0.00
PRIMER_INTERNAL_163_SELF_END_TH=0.00
PRIMER_INTERNAL_163_HAIRPIN_TH=42.01
PRIMER_INTERNAL_164_PENALTY=8.787348
PRIMER_INTERNAL_164_SEQUENCE=CACACACACAGCACACAGGTATTAGTG
PRIMER_INTERNAL_164=40,27
PRIMER_INTERNAL_164_TM=58.213
PRIMER_INTERNAL_164_GC_PERCENT=48.148
PRIMER_INTERNAL_164_SELF_ANY_TH=0.00
PRIMER_INTERNAL_164_SELF_END_TH=0.00
PRIMER_INTERNAL_164_HAIRPIN_TH=39.84
PRIMER_INTERNAL_165_PENALTY=8.787348
PRIMER_INTERNAL_165_SEQUENCE=CACACACACACAGCACACAGGTATTAG
PRIMER_INTERNAL_165=38,27
PRIMER_INTERNAL_165_TM=58.213
PRIMER_INTERNAL_165_GC_PERCENT=48.148
PRIMER_INTERNAL_165_SELF_ANY_TH=0.00
PRIMER_INTERNAL_165_SELF_END_TH=0.00
PRIMER_INTERNAL_165_HAIRPIN_TH=0.00
PRIMER_INTERNAL_166_PENALTY=8.904972
PRIMER_INTERNAL_166_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_166=64,26
PRIMER_INTERNAL_166_TM=62.905
PRIMER_INTERNAL_166_GC_PERCENT=57.692
PRIMER_INTERNAL_166_SELF_ANY_TH=7.03
PRIMER_INTERNAL_166_SELF_END_TH=0.00
PRIMER_INTERNAL_166_HAIRPIN_TH=43.79
PRIMER_INTERNAL_167_PENALTY=9.679371
PRIMER_INTERNAL_167_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_167=67,27
PRIMER_INTERNAL_167_TM=62.679
PRIMER_INTERNAL_167_GC_PERCENT=55.556
PRIMER_INTERNAL_167_SELF_ANY_TH=0.00
PRIMER_INTERNAL_167_SELF_END_TH=0.00
PRIMER_INTERNAL_167_HAIRPIN_TH=41.68
=
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_pick_primer_list_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=33
PRIMER_RIGHT_NUM_RETURNED=33
PRIMER_INTERNAL_NUM_RETURNED=73
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_LEFT_0=118,20
PRIMER_RIGHT_0=137,20
PRIMER_INTERNAL_0=125,20
PRIMER_LEFT_0_TM=58.514
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=59.906
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_1_PENALTY=1.160593
PRIMER_RIGHT_1_PENALTY=1.160593
PRIMER_INTERNAL_1_PENALTY=0.301069
PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_1=122,19
PRIMER_RIGHT_1=140,19
PRIMER_INTERNAL_1=126,20
PRIMER_LEFT_1_TM=59.161
PRIMER_RIGHT_1_TM=59.161
PRIMER_INTERNAL_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_SELF_ANY_TH=7.82
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=37.44
PRIMER_INTERNAL_1_HAIRPIN_TH=36.42
PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_1_END_STABILITY=5.0300
PRIMER_RIGHT_1_END_STABILITY=2.4400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_2_PENALTY=1.656085
PRIMER_RIGHT_2_PENALTY=1.656085
PRIMER_INTERNAL_2_PENALTY=0.900305
PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA
PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG
PRIMER_LEFT_2=117,21
PRIMER_RIGHT_2=137,21
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=58.344
PRIMER_INTERNAL_2_TM=59.100
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.14
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=5.3400
PRIMER_RIGHT_2_END_STABILITY=1.7500
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=8.6247
PRIMER_LEFT_3_PENALTY=1.695359
PRIMER_RIGHT_3_PENALTY=1.695359
PRIMER_INTERNAL_3_PENALTY=0.956520
PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC
PRIMER_LEFT_3=53,21
PRIMER_RIGHT_3=73,21
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=58.305
PRIMER_RIGHT_3_TM=58.305
PRIMER_INTERNAL_3_TM=59.043
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=36.42
PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.7700
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_4_PENALTY=1.831685
PRIMER_RIGHT_4_PENALTY=1.831685
PRIMER_INTERNAL_4_PENALTY=1.018091
PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA
PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT
PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=84,20
PRIMER_INTERNAL_4=125,21
PRIMER_LEFT_4_TM=57.168
PRIMER_RIGHT_4_TM=57.168
PRIMER_INTERNAL_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=57.143
PRIMER_INTERNAL_4_SELF_ANY_TH=7.82
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=35.45
PRIMER_RIGHT_4_HAIRPIN_TH=31.80
PRIMER_INTERNAL_4_HAIRPIN_TH=36.42
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=2.1500
PRIMER_RIGHT_4_END_STABILITY=4.8400
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.8143
PRIMER_LEFT_5_PENALTY=1.902705
PRIMER_RIGHT_5_PENALTY=1.902705
PRIMER_INTERNAL_5_PENALTY=1.178696
PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA
PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_5=62,20
PRIMER_RIGHT_5=81,20
PRIMER_INTERNAL_5=124,21
PRIMER_LEFT_5_TM=57.097
PRIMER_RIGHT_5_TM=57.097
PRIMER_INTERNAL_5_TM=60.179
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=57.143
PRIMER_INTERNAL_5_SELF_ANY_TH=7.82
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=36.42
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=2.7400
PRIMER_RIGHT_5_END_STABILITY=2.5200
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=29.7752
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_6_PENALTY=1.902705
PRIMER_RIGHT_6_PENALTY=1.902705
PRIMER_INTERNAL_6_PENALTY=1.372876
PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT
PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA
PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_6=61,20
PRIMER_RIGHT_6=80,20
PRIMER_INTERNAL_6=126,21
PRIMER_LEFT_6_TM=57.097
PRIMER_RIGHT_6_TM=57.097
PRIMER_INTERNAL_6_TM=60.373
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=7.82
PRIMER_LEFT_6_SELF_ANY_TH=0.00
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=36.42
PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=3.5500
PRIMER_RIGHT_6_END_STABILITY=2.1000
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.0753
PRIMER_LEFT_7_PENALTY=1.950877
PRIMER_RIGHT_7_PENALTY=1.950877
PRIMER_INTERNAL_7_PENALTY=1.393730
PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG
PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA
PRIMER_LEFT_7=119,19
PRIMER_RIGHT_7=137,19
PRIMER_INTERNAL_7=126,19
PRIMER_LEFT_7_TM=58.049
PRIMER_RIGHT_7_TM=58.049
PRIMER_INTERNAL_7_TM=59.606
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_INTERNAL_7_GC_PERCENT=63.158
PRIMER_INTERNAL_7_SELF_ANY_TH=7.82
PRIMER_LEFT_7_SELF_ANY_TH=0.00
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=0.00
PRIMER_RIGHT_7_HAIRPIN_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=36.42
PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=5.3400
PRIMER_RIGHT_7_END_STABILITY=3.5300
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_8_PENALTY=1.972625
PRIMER_RIGHT_8_PENALTY=1.972625
PRIMER_INTERNAL_8_PENALTY=1.595076
PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA
PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_8=56,21
PRIMER_RIGHT_8=76,21
PRIMER_INTERNAL_8=123,21
PRIMER_LEFT_8_TM=58.027
PRIMER_RIGHT_8_TM=58.027
PRIMER_INTERNAL_8_TM=59.405
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_INTERNAL_8_GC_PERCENT=57.143
PRIMER_INTERNAL_8_SELF_ANY_TH=0.00
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=0.00
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=41.14
PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_LEFT_8_END_STABILITY=3.4100
PRIMER_RIGHT_8_END_STABILITY=3.8600
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=32.2237
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.8066
PRIMER_LEFT_9_PENALTY=1.973945
PRIMER_RIGHT_9_PENALTY=1.973945
PRIMER_INTERNAL_9_PENALTY=2.007622
PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC
PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT
PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_9=55,21
PRIMER_RIGHT_9=75,21
PRIMER_INTERNAL_9=121,22
PRIMER_LEFT_9_TM=58.026
PRIMER_RIGHT_9_TM=58.026
PRIMER_INTERNAL_9_TM=59.992
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_INTERNAL_9_GC_PERCENT=59.091
PRIMER_INTERNAL_9_SELF_ANY_TH=0.00
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=0.00
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=0.00
PRIMER_RIGHT_9_HAIRPIN_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=0.00
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=3.0100
PRIMER_RIGHT_9_END_STABILITY=4.0000
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=27.4392
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=5.5040
PRIMER_LEFT_10_PENALTY=1.973945
PRIMER_RIGHT_10_PENALTY=1.973945
PRIMER_INTERNAL_10_PENALTY=2.235780
PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT
PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC
PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG
PRIMER_LEFT_10=54,21
PRIMER_RIGHT_10=74,21
PRIMER_INTERNAL_10=125,19
PRIMER_LEFT_10_TM=58.026
PRIMER_RIGHT_10_TM=58.026
PRIMER_INTERNAL_10_TM=58.764
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_INTERNAL_10_GC_PERCENT=63.158
PRIMER_INTERNAL_10_SELF_ANY_TH=0.00
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=0.00
PRIMER_RIGHT_10_HAIRPIN_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.14
PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=3.1600
PRIMER_RIGHT_10_END_STABILITY=3.8500
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=19.5725
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=9.9844
PRIMER_LEFT_11_PENALTY=2.120321
PRIMER_RIGHT_11_PENALTY=2.120321
PRIMER_INTERNAL_11_PENALTY=2.240074
PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA
PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT
PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_11=64,21
PRIMER_RIGHT_11=84,21
PRIMER_INTERNAL_11=124,22
PRIMER_LEFT_11_TM=57.880
PRIMER_RIGHT_11_TM=57.880
PRIMER_INTERNAL_11_TM=60.240
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_INTERNAL_11_GC_PERCENT=54.545
PRIMER_INTERNAL_11_SELF_ANY_TH=7.82
PRIMER_LEFT_11_SELF_ANY_TH=0.00
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=35.45
PRIMER_RIGHT_11_HAIRPIN_TH=31.80
PRIMER_INTERNAL_11_HAIRPIN_TH=36.42
PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=2.1500
PRIMER_RIGHT_11_END_STABILITY=3.6000
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8905
PRIMER_LEFT_12_PENALTY=2.142778
PRIMER_RIGHT_12_PENALTY=2.142778
PRIMER_INTERNAL_12_PENALTY=2.299276
PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_12=119,20
PRIMER_RIGHT_12=138,20
PRIMER_INTERNAL_12=122,22
PRIMER_LEFT_12_TM=61.143
PRIMER_RIGHT_12_TM=61.143
PRIMER_INTERNAL_12_TM=60.299
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=59.091
PRIMER_INTERNAL_12_SELF_ANY_TH=0.00
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=0.00
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.14
PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=5.3400
PRIMER_RIGHT_12_END_STABILITY=3.5300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_13_PENALTY=2.299089
PRIMER_RIGHT_13_PENALTY=2.299089
PRIMER_INTERNAL_13_PENALTY=2.427391
PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC
PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_13=123,19
PRIMER_RIGHT_13=141,19
PRIMER_INTERNAL_13=123,22
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.299
PRIMER_INTERNAL_13_TM=60.427
PRIMER_LEFT_13_GC_PERCENT=52.632
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_INTERNAL_13_GC_PERCENT=54.545
PRIMER_INTERNAL_13_SELF_ANY_TH=7.82
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=0.00
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=0.00
PRIMER_RIGHT_13_HAIRPIN_TH=37.44
PRIMER_INTERNAL_13_HAIRPIN_TH=36.42
PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_13_END_STABILITY=4.7000
PRIMER_RIGHT_13_END_STABILITY=2.4300
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_14_PENALTY=2.402628
PRIMER_RIGHT_14_PENALTY=2.402628
PRIMER_INTERNAL_14_PENALTY=2.610811
PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT
PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_14=62,21
PRIMER_RIGHT_14=82,21
PRIMER_INTERNAL_14=125,22
PRIMER_LEFT_14_TM=57.597
PRIMER_RIGHT_14_TM=57.597
PRIMER_INTERNAL_14_TM=60.611
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_INTERNAL_14_GC_PERCENT=59.091
PRIMER_INTERNAL_14_SELF_ANY_TH=7.82
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=0.00
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=0.00
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=36.42
PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=2.2900
PRIMER_RIGHT_14_END_STABILITY=2.5200
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=30.4432
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_15_PENALTY=2.402628
PRIMER_RIGHT_15_PENALTY=2.402628
PRIMER_INTERNAL_15_PENALTY=2.740095
PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT
PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT
PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT
PRIMER_LEFT_15=60,21
PRIMER_RIGHT_15=80,21
PRIMER_INTERNAL_15=127,19
PRIMER_LEFT_15_TM=57.597
PRIMER_RIGHT_15_TM=57.597
PRIMER_INTERNAL_15_TM=58.260
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_INTERNAL_15_GC_PERCENT=63.158
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=0.00
PRIMER_RIGHT_15_HAIRPIN_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=36.42
PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=3.5500
PRIMER_RIGHT_15_END_STABILITY=1.9800
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0593
PRIMER_LEFT_16_PENALTY=2.455619
PRIMER_RIGHT_16_PENALTY=2.455619
PRIMER_INTERNAL_16_PENALTY=2.880381
PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG
PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC
PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_16=63,21
PRIMER_RIGHT_16=83,21
PRIMER_INTERNAL_16=120,22
PRIMER_LEFT_16_TM=57.544
PRIMER_RIGHT_16_TM=57.544
PRIMER_INTERNAL_16_TM=59.120
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_INTERNAL_16_GC_PERCENT=54.545
PRIMER_INTERNAL_16_SELF_ANY_TH=0.00
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=0.00
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=0.00
PRIMER_RIGHT_16_HAIRPIN_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=0.00
PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_LEFT_16_END_STABILITY=2.3900
PRIMER_RIGHT_16_END_STABILITY=2.7800
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=25.0463
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8601
PRIMER_LEFT_17_PENALTY=2.539776
PRIMER_RIGHT_17_PENALTY=2.539776
PRIMER_INTERNAL_17_PENALTY=2.939429
PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC
PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_17=122,20
PRIMER_RIGHT_17=141,20
PRIMER_INTERNAL_17=122,21
PRIMER_LEFT_17_TM=61.540
PRIMER_RIGHT_17_TM=61.540
PRIMER_INTERNAL_17_TM=58.061
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_TH=37.44
PRIMER_INTERNAL_17_HAIRPIN_TH=0.00
PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_17_END_STABILITY=4.7000
PRIMER_RIGHT_17_END_STABILITY=2.4400
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_18_PENALTY=2.539776
PRIMER_RIGHT_18_PENALTY=2.539776
PRIMER_INTERNAL_18_PENALTY=2.939429
PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_18=121,20
PRIMER_RIGHT_18=140,20
PRIMER_INTERNAL_18=121,21
PRIMER_LEFT_18_TM=61.540
PRIMER_RIGHT_18_TM=61.540
PRIMER_INTERNAL_18_TM=58.061
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_INTERNAL_18_GC_PERCENT=57.143
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_TH=37.44
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_18_END_STABILITY=5.0300
PRIMER_RIGHT_18_END_STABILITY=3.2800
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_19_PENALTY=2.587613
PRIMER_RIGHT_19_PENALTY=2.587613
PRIMER_INTERNAL_19_PENALTY=3.476553
PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC
PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_19=120,19
PRIMER_RIGHT_19=138,19
PRIMER_INTERNAL_19=123,23
PRIMER_LEFT_19_TM=60.588
PRIMER_RIGHT_19_TM=60.588
PRIMER_INTERNAL_19_TM=60.477
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_INTERNAL_19_GC_PERCENT=52.174
PRIMER_INTERNAL_19_SELF_ANY_TH=7.82
PRIMER_LEFT_19_SELF_ANY_TH=0.00
PRIMER_RIGHT_19_SELF_ANY_TH=0.00
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=36.42
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_19_END_STABILITY=5.3400
PRIMER_RIGHT_19_END_STABILITY=3.5300
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=2.588667
PRIMER_RIGHT_20_PENALTY=2.588667
PRIMER_INTERNAL_20_PENALTY=3.598972
PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT
PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_20=121,19
PRIMER_RIGHT_20=139,19
PRIMER_INTERNAL_20=119,23
PRIMER_LEFT_20_TM=60.589
PRIMER_RIGHT_20_TM=60.589
PRIMER_INTERNAL_20_TM=59.401
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=52.632
PRIMER_INTERNAL_20_GC_PERCENT=52.174
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=0.00
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=0.00
PRIMER_LEFT_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_TH=37.44
PRIMER_INTERNAL_20_HAIRPIN_TH=0.00
PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_20_END_STABILITY=5.0700
PRIMER_RIGHT_20_END_STABILITY=3.2800
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_21_PENALTY=2.627395
PRIMER_RIGHT_21_PENALTY=2.627395
PRIMER_INTERNAL_21_PENALTY=3.606233
PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA
PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT
PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG
PRIMER_LEFT_21=52,21
PRIMER_RIGHT_21=72,21
PRIMER_INTERNAL_21=126,18
PRIMER_LEFT_21_TM=57.373
PRIMER_RIGHT_21_TM=57.373
PRIMER_INTERNAL_21_TM=58.394
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_INTERNAL_21_GC_PERCENT=66.667
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.14
PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_21_END_STABILITY=4.5200
PRIMER_RIGHT_21_END_STABILITY=3.0600
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=1.1837
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_22_PENALTY=2.678056
PRIMER_RIGHT_22_PENALTY=2.678056
PRIMER_INTERNAL_22_PENALTY=3.829005
PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT
PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA
PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_22=58,21
PRIMER_RIGHT_22=78,21
PRIMER_INTERNAL_22=124,23
PRIMER_LEFT_22_TM=57.322
PRIMER_RIGHT_22_TM=57.322
PRIMER_INTERNAL_22_TM=60.829
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_INTERNAL_22_GC_PERCENT=56.522
PRIMER_INTERNAL_22_SELF_ANY_TH=7.82
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=0.00
PRIMER_RIGHT_22_HAIRPIN_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=36.42
PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_22_END_STABILITY=3.4100
PRIMER_RIGHT_22_END_STABILITY=2.1500
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=25.2513
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=19.0855
PRIMER_LEFT_23_PENALTY=2.780624
PRIMER_RIGHT_23_PENALTY=2.780624
PRIMER_INTERNAL_23_PENALTY=3.865064
PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT
PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_23=62,19
PRIMER_RIGHT_23=80,19
PRIMER_INTERNAL_23=120,21
PRIMER_LEFT_23_TM=57.219
PRIMER_RIGHT_23_TM=57.219
PRIMER_INTERNAL_23_TM=57.135
PRIMER_LEFT_23_GC_PERCENT=47.368
PRIMER_RIGHT_23_GC_PERCENT=47.368
PRIMER_INTERNAL_23_GC_PERCENT=52.381
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=0.00
PRIMER_RIGHT_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_23_END_STABILITY=3.5500
PRIMER_RIGHT_23_END_STABILITY=2.5200
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_24_PENALTY=2.999071
PRIMER_RIGHT_24_PENALTY=2.999071
PRIMER_INTERNAL_24_PENALTY=3.905453
PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA
PRIMER_LEFT_24=120,20
PRIMER_RIGHT_24=139,20
PRIMER_INTERNAL_24=127,18
PRIMER_LEFT_24_TM=61.999
PRIMER_RIGHT_24_TM=61.999
PRIMER_INTERNAL_24_TM=58.095
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_INTERNAL_24_GC_PERCENT=66.667
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=0.00
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=0.00
PRIMER_RIGHT_24_HAIRPIN_TH=37.44
PRIMER_INTERNAL_24_HAIRPIN_TH=36.42
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_24_END_STABILITY=5.0700
PRIMER_RIGHT_24_END_STABILITY=3.5300
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_25_PENALTY=2.999229
PRIMER_RIGHT_25_PENALTY=2.999229
PRIMER_INTERNAL_25_PENALTY=3.954555
PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG
PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC
PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_25=59,21
PRIMER_RIGHT_25=79,21
PRIMER_INTERNAL_25=120,23
PRIMER_LEFT_25_TM=57.001
PRIMER_RIGHT_25_TM=57.001
PRIMER_INTERNAL_25_TM=60.955
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_INTERNAL_25_GC_PERCENT=56.522
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=0.00
PRIMER_RIGHT_25_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=0.00
PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_25_END_STABILITY=3.6600
PRIMER_RIGHT_25_END_STABILITY=2.0800
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=30.7683
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=20.4326
PRIMER_LEFT_26_PENALTY=2.999229
PRIMER_RIGHT_26_PENALTY=2.999229
PRIMER_INTERNAL_26_PENALTY=4.240075
PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC
PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG
PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_26=57,21
PRIMER_RIGHT_26=77,21
PRIMER_INTERNAL_26=122,23
PRIMER_LEFT_26_TM=57.001
PRIMER_RIGHT_26_TM=57.001
PRIMER_INTERNAL_26_TM=61.240
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=56.522
PRIMER_INTERNAL_26_SELF_ANY_TH=7.82
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=0.00
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=0.00
PRIMER_LEFT_26_HAIRPIN_TH=0.00
PRIMER_RIGHT_26_HAIRPIN_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=36.42
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=3.5100
PRIMER_RIGHT_26_END_STABILITY=2.9000
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=20.2809
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.6247
PRIMER_LEFT_27_PENALTY=3.455227
PRIMER_RIGHT_27_PENALTY=3.455227
PRIMER_INTERNAL_27_PENALTY=4.486709
PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_27=118,21
PRIMER_RIGHT_27=138,21
PRIMER_INTERNAL_27=119,22
PRIMER_LEFT_27_TM=61.455
PRIMER_RIGHT_27_TM=61.455
PRIMER_INTERNAL_27_TM=57.513
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=50.000
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=0.00
PRIMER_RIGHT_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=0.00
PRIMER_RIGHT_27_SELF_END_TH=0.00
PRIMER_LEFT_27_HAIRPIN_TH=0.00
PRIMER_RIGHT_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=5.3400
PRIMER_RIGHT_27_END_STABILITY=3.0100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_28_PENALTY=3.677573
PRIMER_RIGHT_28_PENALTY=3.677573
PRIMER_INTERNAL_28_PENALTY=4.513970
PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC
PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_28=123,20
PRIMER_RIGHT_28=142,20
PRIMER_INTERNAL_28=118,24
PRIMER_LEFT_28_TM=62.678
PRIMER_RIGHT_28_TM=62.678
PRIMER_INTERNAL_28_TM=59.486
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_INTERNAL_28_GC_PERCENT=50.000
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=37.44
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_28_END_STABILITY=4.3000
PRIMER_RIGHT_28_END_STABILITY=2.4300
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.1346
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_29_PENALTY=4.100862
PRIMER_RIGHT_29_PENALTY=4.100862
PRIMER_INTERNAL_29_PENALTY=5.029817
PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC
PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC
PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_29=128,19
PRIMER_RIGHT_29=146,19
PRIMER_INTERNAL_29=123,24
PRIMER_LEFT_29_TM=62.101
PRIMER_RIGHT_29_TM=62.101
PRIMER_INTERNAL_29_TM=61.030
PRIMER_LEFT_29_GC_PERCENT=63.158
PRIMER_RIGHT_29_GC_PERCENT=63.158
PRIMER_INTERNAL_29_GC_PERCENT=54.167
PRIMER_INTERNAL_29_SELF_ANY_TH=7.82
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=38.29
PRIMER_RIGHT_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=36.42
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_29_END_STABILITY=3.6900
PRIMER_RIGHT_29_END_STABILITY=3.6800
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0362
PRIMER_LEFT_30_PENALTY=4.194272
PRIMER_RIGHT_30_PENALTY=4.194272
PRIMER_INTERNAL_30_PENALTY=5.052238
PRIMER_LEFT_30_SEQUENCE=AAACGCAAAGCACGCTCCC
PRIMER_RIGHT_30_SEQUENCE=GGGAGCGTGCTTTGCGTTT
PRIMER_INTERNAL_30_SEQUENCE=CCAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_30=124,19
PRIMER_RIGHT_30=142,19
PRIMER_INTERNAL_30=121,23
PRIMER_LEFT_30_TM=62.194
PRIMER_RIGHT_30_TM=62.194
PRIMER_INTERNAL_30_TM=62.052
PRIMER_LEFT_30_GC_PERCENT=57.895
PRIMER_RIGHT_30_GC_PERCENT=57.895
PRIMER_INTERNAL_30_GC_PERCENT=60.870
PRIMER_INTERNAL_30_SELF_ANY_TH=0.00
PRIMER_LEFT_30_SELF_ANY_TH=0.00
PRIMER_RIGHT_30_SELF_ANY_TH=0.00
PRIMER_INTERNAL_30_SELF_END_TH=0.00
PRIMER_LEFT_30_SELF_END_TH=0.00
PRIMER_RIGHT_30_SELF_END_TH=0.00
PRIMER_LEFT_30_HAIRPIN_TH=0.00
PRIMER_RIGHT_30_HAIRPIN_TH=37.44
PRIMER_INTERNAL_30_HAIRPIN_TH=41.14
PRIMER_LEFT_30_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_30_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_30_END_STABILITY=4.3000
PRIMER_RIGHT_30_END_STABILITY=3.6000
PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=9.8734
PRIMER_LEFT_31_PENALTY=4.473143
PRIMER_RIGHT_31_PENALTY=4.473143
PRIMER_INTERNAL_31_PENALTY=5.152054
PRIMER_LEFT_31_SEQUENCE=TTCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_31_SEQUENCE=AGCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_31_SEQUENCE=TTCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_31=119,21
PRIMER_RIGHT_31=139,21
PRIMER_INTERNAL_31=119,24
PRIMER_LEFT_31_TM=62.473
PRIMER_RIGHT_31_TM=62.473
PRIMER_INTERNAL_31_TM=61.152
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_INTERNAL_31_GC_PERCENT=54.167
PRIMER_INTERNAL_31_SELF_ANY_TH=0.00
PRIMER_LEFT_31_SELF_ANY_TH=0.00
PRIMER_RIGHT_31_SELF_ANY_TH=0.00
PRIMER_INTERNAL_31_SELF_END_TH=0.00
PRIMER_LEFT_31_SELF_END_TH=0.00
PRIMER_RIGHT_31_SELF_END_TH=0.00
PRIMER_LEFT_31_HAIRPIN_TH=0.00
PRIMER_RIGHT_31_HAIRPIN_TH=37.44
PRIMER_INTERNAL_31_HAIRPIN_TH=0.00
PRIMER_LEFT_31_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_31_END_STABILITY=5.0700
PRIMER_RIGHT_31_END_STABILITY=3.5300
PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_32_PENALTY=4.837196
PRIMER_RIGHT_32_PENALTY=4.837196
PRIMER_INTERNAL_32_PENALTY=5.256814
PRIMER_LEFT_32_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_32_SEQUENCE=GAGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_32_SEQUENCE=CAAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_32=120,21
PRIMER_RIGHT_32=140,21
PRIMER_INTERNAL_32=122,24
PRIMER_LEFT_32_TM=62.837
PRIMER_RIGHT_32_TM=62.837
PRIMER_INTERNAL_32_TM=61.257
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_INTERNAL_32_GC_PERCENT=54.167
PRIMER_INTERNAL_32_SELF_ANY_TH=7.82
PRIMER_LEFT_32_SELF_ANY_TH=0.00
PRIMER_RIGHT_32_SELF_ANY_TH=0.00
PRIMER_INTERNAL_32_SELF_END_TH=0.00
PRIMER_LEFT_32_SELF_END_TH=0.00
PRIMER_RIGHT_32_SELF_END_TH=0.00
PRIMER_LEFT_32_HAIRPIN_TH=0.00
PRIMER_RIGHT_32_HAIRPIN_TH=37.44
PRIMER_INTERNAL_32_HAIRPIN_TH=36.42
PRIMER_LEFT_32_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_32_END_STABILITY=5.0300
PRIMER_RIGHT_32_END_STABILITY=3.5300
PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_INTERNAL_33_PENALTY=5.325970
PRIMER_INTERNAL_33_SEQUENCE=ATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_33=118,23
PRIMER_INTERNAL_33_TM=57.674
PRIMER_INTERNAL_33_GC_PERCENT=47.826
PRIMER_INTERNAL_33_SELF_ANY_TH=0.00
PRIMER_INTERNAL_33_SELF_END_TH=0.00
PRIMER_INTERNAL_33_HAIRPIN_TH=0.00
PRIMER_INTERNAL_34_PENALTY=5.931698
PRIMER_INTERNAL_34_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_34=117,25
PRIMER_INTERNAL_34_TM=59.068
PRIMER_INTERNAL_34_GC_PERCENT=48.000
PRIMER_INTERNAL_34_SELF_ANY_TH=0.00
PRIMER_INTERNAL_34_SELF_END_TH=0.00
PRIMER_INTERNAL_34_HAIRPIN_TH=0.00
PRIMER_INTERNAL_35_PENALTY=6.171494
PRIMER_INTERNAL_35_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_35=118,25
PRIMER_INTERNAL_35_TM=61.171
PRIMER_INTERNAL_35_GC_PERCENT=52.000
PRIMER_INTERNAL_35_SELF_ANY_TH=0.00
PRIMER_INTERNAL_35_SELF_END_TH=0.00
PRIMER_INTERNAL_35_HAIRPIN_TH=0.00
PRIMER_INTERNAL_36_PENALTY=6.354311
PRIMER_INTERNAL_36_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_36=116,26
PRIMER_INTERNAL_36_TM=59.646
PRIMER_INTERNAL_36_GC_PERCENT=50.000
PRIMER_INTERNAL_36_SELF_ANY_TH=0.00
PRIMER_INTERNAL_36_SELF_END_TH=0.00
PRIMER_INTERNAL_36_HAIRPIN_TH=0.00
PRIMER_INTERNAL_37_PENALTY=6.689016
PRIMER_INTERNAL_37_SEQUENCE=TATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_37=117,24
PRIMER_INTERNAL_37_TM=57.311
PRIMER_INTERNAL_37_GC_PERCENT=45.833
PRIMER_INTERNAL_37_SELF_ANY_TH=0.00
PRIMER_INTERNAL_37_SELF_END_TH=0.00
PRIMER_INTERNAL_37_HAIRPIN_TH=0.00
PRIMER_INTERNAL_38_PENALTY=6.689016
PRIMER_INTERNAL_38_SEQUENCE=TCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_38=115,24
PRIMER_INTERNAL_38_TM=57.311
PRIMER_INTERNAL_38_GC_PERCENT=45.833
PRIMER_INTERNAL_38_SELF_ANY_TH=0.00
PRIMER_INTERNAL_38_SELF_END_TH=0.00
PRIMER_INTERNAL_38_HAIRPIN_TH=0.00
PRIMER_INTERNAL_39_PENALTY=6.694952
PRIMER_INTERNAL_39_SEQUENCE=CTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_39=116,24
PRIMER_INTERNAL_39_TM=57.305
PRIMER_INTERNAL_39_GC_PERCENT=45.833
PRIMER_INTERNAL_39_SELF_ANY_TH=0.00
PRIMER_INTERNAL_39_SELF_END_TH=0.00
PRIMER_INTERNAL_39_HAIRPIN_TH=0.00
PRIMER_INTERNAL_40_PENALTY=6.707310
PRIMER_INTERNAL_40_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_40=117,26
PRIMER_INTERNAL_40_TM=60.707
PRIMER_INTERNAL_40_GC_PERCENT=50.000
PRIMER_INTERNAL_40_SELF_ANY_TH=0.00
PRIMER_INTERNAL_40_SELF_END_TH=0.00
PRIMER_INTERNAL_40_HAIRPIN_TH=0.00
PRIMER_INTERNAL_41_PENALTY=6.724062
PRIMER_INTERNAL_41_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_41=115,25
PRIMER_INTERNAL_41_TM=58.276
PRIMER_INTERNAL_41_GC_PERCENT=44.000
PRIMER_INTERNAL_41_SELF_ANY_TH=0.00
PRIMER_INTERNAL_41_SELF_END_TH=0.00
PRIMER_INTERNAL_41_HAIRPIN_TH=0.00
PRIMER_INTERNAL_42_PENALTY=6.744217
PRIMER_INTERNAL_42_SEQUENCE=CAAAACGCAAAGCACGCTCCCGATC
PRIMER_INTERNAL_42=122,25
PRIMER_INTERNAL_42_TM=61.744
PRIMER_INTERNAL_42_GC_PERCENT=56.000
PRIMER_INTERNAL_42_SELF_ANY_TH=7.82
PRIMER_INTERNAL_42_SELF_END_TH=0.00
PRIMER_INTERNAL_42_HAIRPIN_TH=36.42
PRIMER_INTERNAL_43_PENALTY=6.785590
PRIMER_INTERNAL_43_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_43=114,25
PRIMER_INTERNAL_43_TM=58.214
PRIMER_INTERNAL_43_GC_PERCENT=48.000
PRIMER_INTERNAL_43_SELF_ANY_TH=0.00
PRIMER_INTERNAL_43_SELF_END_TH=0.00
PRIMER_INTERNAL_43_HAIRPIN_TH=0.00
PRIMER_INTERNAL_44_PENALTY=6.889273
PRIMER_INTERNAL_44_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_44=114,26
PRIMER_INTERNAL_44_TM=59.111
PRIMER_INTERNAL_44_GC_PERCENT=46.154
PRIMER_INTERNAL_44_SELF_ANY_TH=0.00
PRIMER_INTERNAL_44_SELF_END_TH=0.00
PRIMER_INTERNAL_44_HAIRPIN_TH=0.00
PRIMER_INTERNAL_45_PENALTY=6.889273
PRIMER_INTERNAL_45_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_45=113,26
PRIMER_INTERNAL_45_TM=59.111
PRIMER_INTERNAL_45_GC_PERCENT=46.154
PRIMER_INTERNAL_45_SELF_ANY_TH=0.00
PRIMER_INTERNAL_45_SELF_END_TH=0.00
PRIMER_INTERNAL_45_HAIRPIN_TH=0.00
PRIMER_INTERNAL_46_PENALTY=6.910113
PRIMER_INTERNAL_46_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGA
PRIMER_INTERNAL_46=121,24
PRIMER_INTERNAL_46_TM=62.910
PRIMER_INTERNAL_46_GC_PERCENT=58.333
PRIMER_INTERNAL_46_SELF_ANY_TH=7.82
PRIMER_INTERNAL_46_SELF_END_TH=0.00
PRIMER_INTERNAL_46_HAIRPIN_TH=36.42
PRIMER_INTERNAL_47_PENALTY=6.910113
PRIMER_INTERNAL_47_SEQUENCE=TCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_47=120,24
PRIMER_INTERNAL_47_TM=62.910
PRIMER_INTERNAL_47_GC_PERCENT=58.333
PRIMER_INTERNAL_47_SELF_ANY_TH=0.00
PRIMER_INTERNAL_47_SELF_END_TH=0.00
PRIMER_INTERNAL_47_HAIRPIN_TH=41.14
PRIMER_INTERNAL_48_PENALTY=7.008353
PRIMER_INTERNAL_48_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_48=116,25
PRIMER_INTERNAL_48_TM=57.992
PRIMER_INTERNAL_48_GC_PERCENT=48.000
PRIMER_INTERNAL_48_SELF_ANY_TH=0.00
PRIMER_INTERNAL_48_SELF_END_TH=0.00
PRIMER_INTERNAL_48_HAIRPIN_TH=0.00
PRIMER_INTERNAL_49_PENALTY=7.035358
PRIMER_INTERNAL_49_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_49=113,27
PRIMER_INTERNAL_49_TM=59.965
PRIMER_INTERNAL_49_GC_PERCENT=44.444
PRIMER_INTERNAL_49_SELF_ANY_TH=0.00
PRIMER_INTERNAL_49_SELF_END_TH=0.00
PRIMER_INTERNAL_49_HAIRPIN_TH=0.00
PRIMER_INTERNAL_50_PENALTY=7.102160
PRIMER_INTERNAL_50_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_50=115,26
PRIMER_INTERNAL_50_TM=58.898
PRIMER_INTERNAL_50_GC_PERCENT=46.154
PRIMER_INTERNAL_50_SELF_ANY_TH=0.00
PRIMER_INTERNAL_50_SELF_END_TH=0.00
PRIMER_INTERNAL_50_HAIRPIN_TH=0.00
PRIMER_INTERNAL_51_PENALTY=7.334450
PRIMER_INTERNAL_51_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_51=114,27
PRIMER_INTERNAL_51_TM=59.666
PRIMER_INTERNAL_51_GC_PERCENT=48.148
PRIMER_INTERNAL_51_SELF_ANY_TH=0.00
PRIMER_INTERNAL_51_SELF_END_TH=0.00
PRIMER_INTERNAL_51_HAIRPIN_TH=0.00
PRIMER_INTERNAL_52_PENALTY=7.480679
PRIMER_INTERNAL_52_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_52=115,27
PRIMER_INTERNAL_52_TM=60.481
PRIMER_INTERNAL_52_GC_PERCENT=48.148
PRIMER_INTERNAL_52_SELF_ANY_TH=0.00
PRIMER_INTERNAL_52_SELF_END_TH=0.00
PRIMER_INTERNAL_52_HAIRPIN_TH=0.00
PRIMER_INTERNAL_53_PENALTY=7.647244
PRIMER_INTERNAL_53_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_53=112,27
PRIMER_INTERNAL_53_TM=59.353
PRIMER_INTERNAL_53_GC_PERCENT=44.444
PRIMER_INTERNAL_53_SELF_ANY_TH=0.00
PRIMER_INTERNAL_53_SELF_END_TH=0.00
PRIMER_INTERNAL_53_HAIRPIN_TH=0.00
PRIMER_INTERNAL_54_PENALTY=7.744113
PRIMER_INTERNAL_54_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_54=55,26
PRIMER_INTERNAL_54_TM=58.256
PRIMER_INTERNAL_54_GC_PERCENT=46.154
PRIMER_INTERNAL_54_SELF_ANY_TH=0.00
PRIMER_INTERNAL_54_SELF_END_TH=0.00
PRIMER_INTERNAL_54_HAIRPIN_TH=0.00
PRIMER_INTERNAL_55_PENALTY=7.867066
PRIMER_INTERNAL_55_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGAT
PRIMER_INTERNAL_55=121,25
PRIMER_INTERNAL_55_TM=62.867
PRIMER_INTERNAL_55_GC_PERCENT=56.000
PRIMER_INTERNAL_55_SELF_ANY_TH=7.82
PRIMER_INTERNAL_55_SELF_END_TH=0.00
PRIMER_INTERNAL_55_HAIRPIN_TH=36.42
PRIMER_INTERNAL_56_PENALTY=7.916609
PRIMER_INTERNAL_56_SEQUENCE=TGACCTGATAGAAACGCCAACTCAC
PRIMER_INTERNAL_56=53,25
PRIMER_INTERNAL_56_TM=57.083
PRIMER_INTERNAL_56_GC_PERCENT=48.000
PRIMER_INTERNAL_56_SELF_ANY_TH=0.00
PRIMER_INTERNAL_56_SELF_END_TH=0.00
PRIMER_INTERNAL_56_HAIRPIN_TH=0.00
PRIMER_INTERNAL_57_PENALTY=7.922893
PRIMER_INTERNAL_57_SEQUENCE=CCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_57=56,25
PRIMER_INTERNAL_57_TM=57.077
PRIMER_INTERNAL_57_GC_PERCENT=48.000
PRIMER_INTERNAL_57_SELF_ANY_TH=0.00
PRIMER_INTERNAL_57_SELF_END_TH=0.00
PRIMER_INTERNAL_57_HAIRPIN_TH=0.00
PRIMER_INTERNAL_58_PENALTY=7.922893
PRIMER_INTERNAL_58_SEQUENCE=ACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_58=55,25
PRIMER_INTERNAL_58_TM=57.077
PRIMER_INTERNAL_58_GC_PERCENT=48.000
PRIMER_INTERNAL_58_SELF_ANY_TH=0.00
PRIMER_INTERNAL_58_SELF_END_TH=0.00
PRIMER_INTERNAL_58_HAIRPIN_TH=0.00
PRIMER_INTERNAL_59_PENALTY=7.963894
PRIMER_INTERNAL_59_SEQUENCE=TGACCTGATAGAAACGCCAACTCACT
PRIMER_INTERNAL_59=53,26
PRIMER_INTERNAL_59_TM=58.036
PRIMER_INTERNAL_59_GC_PERCENT=46.154
PRIMER_INTERNAL_59_SELF_ANY_TH=0.00
PRIMER_INTERNAL_59_SELF_END_TH=0.00
PRIMER_INTERNAL_59_HAIRPIN_TH=0.00
PRIMER_INTERNAL_60_PENALTY=8.131843
PRIMER_INTERNAL_60_SEQUENCE=TGACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_60=53,27
PRIMER_INTERNAL_60_TM=58.868
PRIMER_INTERNAL_60_GC_PERCENT=48.148
PRIMER_INTERNAL_60_SELF_ANY_TH=0.00
PRIMER_INTERNAL_60_SELF_END_TH=0.00
PRIMER_INTERNAL_60_HAIRPIN_TH=0.00
PRIMER_INTERNAL_61_PENALTY=8.132460
PRIMER_INTERNAL_61_SEQUENCE=GACCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_61=54,27
PRIMER_INTERNAL_61_TM=58.868
PRIMER_INTERNAL_61_GC_PERCENT=48.148
PRIMER_INTERNAL_61_SELF_ANY_TH=0.00
PRIMER_INTERNAL_61_SELF_END_TH=0.00
PRIMER_INTERNAL_61_HAIRPIN_TH=0.00
PRIMER_INTERNAL_62_PENALTY=8.193320
PRIMER_INTERNAL_62_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_62=116,27
PRIMER_INTERNAL_62_TM=61.193
PRIMER_INTERNAL_62_GC_PERCENT=51.852
PRIMER_INTERNAL_62_SELF_ANY_TH=0.00
PRIMER_INTERNAL_62_SELF_END_TH=0.00
PRIMER_INTERNAL_62_HAIRPIN_TH=0.00
PRIMER_INTERNAL_63_PENALTY=8.237701
PRIMER_INTERNAL_63_SEQUENCE=GACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_63=54,26
PRIMER_INTERNAL_63_TM=57.762
PRIMER_INTERNAL_63_GC_PERCENT=50.000
PRIMER_INTERNAL_63_SELF_ANY_TH=0.00
PRIMER_INTERNAL_63_SELF_END_TH=0.00
PRIMER_INTERNAL_63_HAIRPIN_TH=0.00
PRIMER_INTERNAL_64_PENALTY=8.755267
PRIMER_INTERNAL_64_SEQUENCE=ATGACCTGATAGAAACGCCAACTCAC
PRIMER_INTERNAL_64=52,26
PRIMER_INTERNAL_64_TM=57.245
PRIMER_INTERNAL_64_GC_PERCENT=46.154
PRIMER_INTERNAL_64_SELF_ANY_TH=0.00
PRIMER_INTERNAL_64_SELF_END_TH=0.00
PRIMER_INTERNAL_64_HAIRPIN_TH=0.00
PRIMER_INTERNAL_65_PENALTY=8.758760
PRIMER_INTERNAL_65_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACG
PRIMER_INTERNAL_65=112,26
PRIMER_INTERNAL_65_TM=57.241
PRIMER_INTERNAL_65_GC_PERCENT=42.308
PRIMER_INTERNAL_65_SELF_ANY_TH=0.00
PRIMER_INTERNAL_65_SELF_END_TH=0.00
PRIMER_INTERNAL_65_HAIRPIN_TH=0.00
PRIMER_INTERNAL_66_PENALTY=8.838967
PRIMER_INTERNAL_66_SEQUENCE=ATGACCTGATAGAAACGCCAACTCACT
PRIMER_INTERNAL_66=52,27
PRIMER_INTERNAL_66_TM=58.161
PRIMER_INTERNAL_66_GC_PERCENT=44.444
PRIMER_INTERNAL_66_SELF_ANY_TH=0.00
PRIMER_INTERNAL_66_SELF_END_TH=0.00
PRIMER_INTERNAL_66_HAIRPIN_TH=0.00
PRIMER_INTERNAL_67_PENALTY=8.978186
PRIMER_INTERNAL_67_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_67=118,26
PRIMER_INTERNAL_67_TM=62.978
PRIMER_INTERNAL_67_GC_PERCENT=53.846
PRIMER_INTERNAL_67_SELF_ANY_TH=0.00
PRIMER_INTERNAL_67_SELF_END_TH=0.00
PRIMER_INTERNAL_67_HAIRPIN_TH=41.14
PRIMER_INTERNAL_68_PENALTY=9.099564
PRIMER_INTERNAL_68_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGTA
PRIMER_INTERNAL_68=55,27
PRIMER_INTERNAL_68_TM=57.900
PRIMER_INTERNAL_68_GC_PERCENT=44.444
PRIMER_INTERNAL_68_SELF_ANY_TH=0.00
PRIMER_INTERNAL_68_SELF_END_TH=0.00
PRIMER_INTERNAL_68_HAIRPIN_TH=0.00
PRIMER_INTERNAL_69_PENALTY=9.448747
PRIMER_INTERNAL_69_SEQUENCE=AAAGTCTATTCCAAAACGCAAAGCACG
PRIMER_INTERNAL_69=111,27
PRIMER_INTERNAL_69_TM=57.551
PRIMER_INTERNAL_69_GC_PERCENT=40.741
PRIMER_INTERNAL_69_SELF_ANY_TH=0.00
PRIMER_INTERNAL_69_SELF_END_TH=0.00
PRIMER_INTERNAL_69_HAIRPIN_TH=0.00
PRIMER_INTERNAL_70_PENALTY=9.472089
PRIMER_INTERNAL_70_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_70=117,27
PRIMER_INTERNAL_70_TM=62.472
PRIMER_INTERNAL_70_GC_PERCENT=51.852
PRIMER_INTERNAL_70_SELF_ANY_TH=0.00
PRIMER_INTERNAL_70_SELF_END_TH=0.00
PRIMER_INTERNAL_70_HAIRPIN_TH=41.14
PRIMER_INTERNAL_71_PENALTY=9.912698
PRIMER_INTERNAL_71_SEQUENCE=CGTTGTCAAAGTCTATTCCAAAACGCA
PRIMER_INTERNAL_71=104,27
PRIMER_INTERNAL_71_TM=57.087
PRIMER_INTERNAL_71_GC_PERCENT=40.741
PRIMER_INTERNAL_71_SELF_ANY_TH=0.00
PRIMER_INTERNAL_71_SELF_END_TH=0.00
PRIMER_INTERNAL_71_HAIRPIN_TH=26.69
PRIMER_INTERNAL_72_PENALTY=9.914131
PRIMER_INTERNAL_72_SEQUENCE=ACGTTGTCAAAGTCTATTCCAAAACGC
PRIMER_INTERNAL_72=103,27
PRIMER_INTERNAL_72_TM=57.086
PRIMER_INTERNAL_72_GC_PERCENT=40.741
PRIMER_INTERNAL_72_SELF_ANY_TH=0.00
PRIMER_INTERNAL_72_SELF_END_TH=0.00
PRIMER_INTERNAL_72_HAIRPIN_TH=35.24
=
PRIMER_TASK=pick_primer_list
PRIMER_PRODUCT_SIZE_RANGE=75-200
P3_FILE_FLAG=1
SEQUENCE_ID=pick_primer_list_and_file_flag_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52
PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 17, high repeat similarity 3, ok 34
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=52
PRIMER_RIGHT_NUM_RETURNED=34
PRIMER_INTERNAL_NUM_RETURNED=168
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.527529
PRIMER_RIGHT_0_PENALTY=0.527529
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=80,20
PRIMER_INTERNAL_0=66,20
PRIMER_LEFT_0_TM=59.528
PRIMER_RIGHT_0_TM=59.528
PRIMER_INTERNAL_0_TM=59.273
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_LEFT_0_SELF_ANY_TH=10.70
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=40.47
PRIMER_RIGHT_0_HAIRPIN_TH=41.64
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_RIGHT_0_END_STABILITY=2.2400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266
PRIMER_LEFT_1_PENALTY=0.606923
PRIMER_RIGHT_1_PENALTY=0.606923
PRIMER_INTERNAL_1_PENALTY=1.416589
PRIMER_LEFT_1_SEQUENCE=CCCGACCCAAATCGATAGCT
PRIMER_RIGHT_1_SEQUENCE=AGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC
PRIMER_LEFT_1=78,20
PRIMER_RIGHT_1=97,20
PRIMER_INTERNAL_1=64,21
PRIMER_LEFT_1_TM=59.607
PRIMER_RIGHT_1_TM=59.607
PRIMER_INTERNAL_1_TM=59.583
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_SELF_ANY_TH=7.03
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=1.71
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.13
PRIMER_RIGHT_1_HAIRPIN_TH=41.74
PRIMER_INTERNAL_1_HAIRPIN_TH=41.60
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_1_END_STABILITY=3.3200
PRIMER_RIGHT_1_END_STABILITY=5.1400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_2_PENALTY=0.608403
PRIMER_RIGHT_2_PENALTY=0.608403
PRIMER_INTERNAL_2_PENALTY=1.438741
PRIMER_LEFT_2_SEQUENCE=TCCCGACCCAAATCGATAGC
PRIMER_RIGHT_2_SEQUENCE=GCTATCGATTTGGGTCGGGA
PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA
PRIMER_LEFT_2=77,20
PRIMER_RIGHT_2=96,20
PRIMER_INTERNAL_2=67,20
PRIMER_LEFT_2_TM=59.608
PRIMER_RIGHT_2_TM=59.608
PRIMER_INTERNAL_2_TM=58.561
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=1.71
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=45.13
PRIMER_RIGHT_2_HAIRPIN_TH=41.74
PRIMER_INTERNAL_2_HAIRPIN_TH=41.60
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_2_END_STABILITY=2.9700
PRIMER_RIGHT_2_END_STABILITY=5.1400
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=2.4428
PRIMER_LEFT_3_PENALTY=0.713597
PRIMER_RIGHT_3_PENALTY=0.713597
PRIMER_INTERNAL_3_PENALTY=1.438741
PRIMER_LEFT_3_SEQUENCE=GACATGACACTGACGATGCA
PRIMER_RIGHT_3_SEQUENCE=TGCATCGTCAGTGTCATGTC
PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC
PRIMER_LEFT_3=11,20
PRIMER_RIGHT_3=30,20
PRIMER_INTERNAL_3=65,20
PRIMER_LEFT_3_TM=58.286
PRIMER_RIGHT_3_TM=58.286
PRIMER_INTERNAL_3_TM=58.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=41.60
PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_3_END_STABILITY=3.9600
PRIMER_RIGHT_3_END_STABILITY=3.0600
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=16.7219
PRIMER_LEFT_4_PENALTY=0.966496
PRIMER_RIGHT_4_PENALTY=0.966496
PRIMER_INTERNAL_4_PENALTY=1.650306
PRIMER_LEFT_4_SEQUENCE=CGATCCCGACCCAAATCGAT
PRIMER_RIGHT_4_SEQUENCE=ATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA
PRIMER_LEFT_4=74,20
PRIMER_RIGHT_4=93,20
PRIMER_INTERNAL_4=66,21
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.966
PRIMER_INTERNAL_4_TM=60.650
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_INTERNAL_4_SELF_ANY_TH=2.18
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=45.13
PRIMER_RIGHT_4_HAIRPIN_TH=41.74
PRIMER_INTERNAL_4_HAIRPIN_TH=41.60
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_4_END_STABILITY=3.5900
PRIMER_RIGHT_4_END_STABILITY=3.6900
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_5_PENALTY=0.967319
PRIMER_RIGHT_5_PENALTY=1.001812
PRIMER_INTERNAL_5_PENALTY=1.650306
PRIMER_LEFT_5_SEQUENCE=CATTCGATCCCGACCCAAAT
PRIMER_RIGHT_5_SEQUENCE=GTCTGCATCGTCAGTGTCAT
PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC
PRIMER_LEFT_5=70,20
PRIMER_RIGHT_5=33,20
PRIMER_INTERNAL_5=65,21
PRIMER_LEFT_5_TM=58.033
PRIMER_RIGHT_5_TM=57.998
PRIMER_INTERNAL_5_TM=60.650
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_INTERNAL_5_GC_PERCENT=66.667
PRIMER_INTERNAL_5_SELF_ANY_TH=0.00
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=45.50
PRIMER_RIGHT_5_HAIRPIN_TH=35.63
PRIMER_INTERNAL_5_HAIRPIN_TH=41.60
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_5_END_STABILITY=2.3200
PRIMER_RIGHT_5_END_STABILITY=3.0600
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=8.6039
PRIMER_LEFT_6_PENALTY=1.000429
PRIMER_RIGHT_6_PENALTY=1.001812
PRIMER_INTERNAL_6_PENALTY=2.095978
PRIMER_LEFT_6_SEQUENCE=CATGACACTGACGATGCAGA
PRIMER_RIGHT_6_SEQUENCE=GCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA
PRIMER_LEFT_6=13,20
PRIMER_RIGHT_6=29,20
PRIMER_INTERNAL_6=67,21
PRIMER_LEFT_6_TM=58.000
PRIMER_RIGHT_6_TM=57.998
PRIMER_INTERNAL_6_TM=58.904
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=0.00
PRIMER_LEFT_6_SELF_ANY_TH=10.65
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=39.20
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=41.60
PRIMER_LEFT_6_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_6_END_STABILITY=4.2600
PRIMER_RIGHT_6_END_STABILITY=3.4100
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=1.2632
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_7_PENALTY=1.001812
PRIMER_RIGHT_7_PENALTY=1.142220
PRIMER_INTERNAL_7_PENALTY=2.360266
PRIMER_LEFT_7_SEQUENCE=ATGACACTGACGATGCAGAC
PRIMER_RIGHT_7_SEQUENCE=GGGATCGAATGGCCCACTA
PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_7=14,20
PRIMER_RIGHT_7=80,19
PRIMER_INTERNAL_7=63,21
PRIMER_LEFT_7_TM=57.998
PRIMER_RIGHT_7_TM=58.858
PRIMER_INTERNAL_7_TM=58.640
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=57.895
PRIMER_INTERNAL_7_GC_PERCENT=61.905
PRIMER_INTERNAL_7_SELF_ANY_TH=7.03
PRIMER_LEFT_7_SELF_ANY_TH=10.65
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=39.20
PRIMER_RIGHT_7_HAIRPIN_TH=41.64
PRIMER_INTERNAL_7_HAIRPIN_TH=41.60
PRIMER_LEFT_7_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_7_END_STABILITY=3.5100
PRIMER_RIGHT_7_END_STABILITY=2.7400
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=16.1639
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=7.8928
PRIMER_LEFT_8_PENALTY=1.001812
PRIMER_RIGHT_8_PENALTY=1.316792
PRIMER_INTERNAL_8_PENALTY=2.456420
PRIMER_LEFT_8_SEQUENCE=AGACATGACACTGACGATGC
PRIMER_RIGHT_8_SEQUENCE=CGTAGCTATCGATTTGGGTCG
PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA
PRIMER_LEFT_8=10,20
PRIMER_RIGHT_8=100,21
PRIMER_INTERNAL_8=63,20
PRIMER_LEFT_8_TM=57.998
PRIMER_RIGHT_8_TM=58.683
PRIMER_INTERNAL_8_TM=57.544
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_INTERNAL_8_SELF_ANY_TH=7.03
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=1.71
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=42.74
PRIMER_INTERNAL_8_HAIRPIN_TH=39.49
PRIMER_LEFT_8_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_8_END_STABILITY=3.9100
PRIMER_RIGHT_8_END_STABILITY=4.7900
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=7.5573
PRIMER_LEFT_9_PENALTY=1.107410
PRIMER_RIGHT_9_PENALTY=1.383135
PRIMER_INTERNAL_9_PENALTY=2.511372
PRIMER_LEFT_9_SEQUENCE=CCATTCGATCCCGACCCAAA
PRIMER_RIGHT_9_SEQUENCE=TAGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC
PRIMER_LEFT_9=69,20
PRIMER_RIGHT_9=98,21
PRIMER_INTERNAL_9=64,20
PRIMER_LEFT_9_TM=60.107
PRIMER_RIGHT_9_TM=59.383
PRIMER_INTERNAL_9_TM=57.489
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_INTERNAL_9_GC_PERCENT=65.000
PRIMER_INTERNAL_9_SELF_ANY_TH=7.03
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=1.71
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=45.50
PRIMER_RIGHT_9_HAIRPIN_TH=41.74
PRIMER_INTERNAL_9_HAIRPIN_TH=41.60
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_9_END_STABILITY=3.2800
PRIMER_RIGHT_9_END_STABILITY=5.1400
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_10_PENALTY=1.126409
PRIMER_RIGHT_10_PENALTY=1.559940
PRIMER_INTERNAL_10_PENALTY=2.626619
PRIMER_LEFT_10_SEQUENCE=CATTCGATCCCGACCCAAATC
PRIMER_RIGHT_10_SEQUENCE=TCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_10=70,21
PRIMER_RIGHT_10=92,19
PRIMER_INTERNAL_10=63,22
PRIMER_LEFT_10_TM=59.126
PRIMER_RIGHT_10_TM=59.560
PRIMER_INTERNAL_10_TM=60.627
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=57.895
PRIMER_INTERNAL_10_GC_PERCENT=63.636
PRIMER_INTERNAL_10_SELF_ANY_TH=7.03
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=45.50
PRIMER_RIGHT_10_HAIRPIN_TH=41.74
PRIMER_INTERNAL_10_HAIRPIN_TH=41.60
PRIMER_LEFT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_10_END_STABILITY=2.1700
PRIMER_RIGHT_10_END_STABILITY=3.6900
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_11_PENALTY=1.142220
PRIMER_RIGHT_11_PENALTY=1.559940
PRIMER_INTERNAL_11_PENALTY=2.783921
PRIMER_LEFT_11_SEQUENCE=TAGTGGGCCATTCGATCCC
PRIMER_RIGHT_11_SEQUENCE=CGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_11=62,19
PRIMER_RIGHT_11=91,19
PRIMER_INTERNAL_11=67,22
PRIMER_LEFT_11_TM=58.858
PRIMER_RIGHT_11_TM=59.560
PRIMER_INTERNAL_11_TM=59.216
PRIMER_LEFT_11_GC_PERCENT=57.895
PRIMER_RIGHT_11_GC_PERCENT=57.895
PRIMER_INTERNAL_11_GC_PERCENT=59.091
PRIMER_INTERNAL_11_SELF_ANY_TH=0.00
PRIMER_LEFT_11_SELF_ANY_TH=10.70
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=40.47
PRIMER_RIGHT_11_HAIRPIN_TH=42.82
PRIMER_INTERNAL_11_HAIRPIN_TH=41.60
PRIMER_LEFT_11_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_11_END_STABILITY=3.8500
PRIMER_RIGHT_11_END_STABILITY=3.5900
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_12_PENALTY=1.179094
PRIMER_RIGHT_12_PENALTY=1.568623
PRIMER_INTERNAL_12_PENALTY=2.885177
PRIMER_LEFT_12_SEQUENCE=TTCGATCCCGACCCAAATCG
PRIMER_RIGHT_12_SEQUENCE=GGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA
PRIMER_LEFT_12=72,20
PRIMER_RIGHT_12=79,20
PRIMER_INTERNAL_12=66,22
PRIMER_LEFT_12_TM=60.179
PRIMER_RIGHT_12_TM=57.431
PRIMER_INTERNAL_12_TM=60.885
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=63.636
PRIMER_INTERNAL_12_SELF_ANY_TH=2.18
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=45.50
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.60
PRIMER_LEFT_12_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_12_END_STABILITY=3.3400
PRIMER_RIGHT_12_END_STABILITY=1.7300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=2.4855
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=4.2003
PRIMER_LEFT_13_PENALTY=1.256117
PRIMER_RIGHT_13_PENALTY=1.673833
PRIMER_INTERNAL_13_PENALTY=3.114431
PRIMER_LEFT_13_SEQUENCE=ATTCGATCCCGACCCAAATC
PRIMER_RIGHT_13_SEQUENCE=GTAGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_13=71,20
PRIMER_RIGHT_13=99,21
PRIMER_INTERNAL_13=62,23
PRIMER_LEFT_13_TM=57.744
PRIMER_RIGHT_13_TM=58.326
PRIMER_INTERNAL_13_TM=60.114
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_INTERNAL_13_GC_PERCENT=60.870
PRIMER_INTERNAL_13_SELF_ANY_TH=7.03
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=1.71
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=45.50
PRIMER_RIGHT_13_HAIRPIN_TH=41.74
PRIMER_INTERNAL_13_HAIRPIN_TH=41.60
PRIMER_LEFT_13_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_13_END_STABILITY=2.1700
PRIMER_RIGHT_13_END_STABILITY=4.7900
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=2.2809
PRIMER_LEFT_14_PENALTY=1.316792
PRIMER_RIGHT_14_PENALTY=1.726673
PRIMER_INTERNAL_14_PENALTY=3.510595
PRIMER_LEFT_14_SEQUENCE=CGACCCAAATCGATAGCTACG
PRIMER_RIGHT_14_SEQUENCE=TGCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC
PRIMER_LEFT_14=80,21
PRIMER_RIGHT_14=30,21
PRIMER_INTERNAL_14=64,22
PRIMER_LEFT_14_TM=58.683
PRIMER_RIGHT_14_TM=59.727
PRIMER_INTERNAL_14_TM=61.511
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_INTERNAL_14_GC_PERCENT=68.182
PRIMER_INTERNAL_14_SELF_ANY_TH=7.03
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=2.30
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=45.13
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=41.60
PRIMER_LEFT_14_LIBRARY_MISPRIMING=10.00, MER4A a consensus
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_14_END_STABILITY=3.5100
PRIMER_RIGHT_14_END_STABILITY=3.4100
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5113
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_15_PENALTY=1.383135
PRIMER_RIGHT_15_PENALTY=1.728621
PRIMER_INTERNAL_15_PENALTY=3.685489
PRIMER_LEFT_15_SEQUENCE=CCCGACCCAAATCGATAGCTA
PRIMER_RIGHT_15_SEQUENCE=TATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_15=78,21
PRIMER_RIGHT_15=94,21
PRIMER_INTERNAL_15=67,23
PRIMER_LEFT_15_TM=59.383
PRIMER_RIGHT_15_TM=59.729
PRIMER_INTERNAL_15_TM=59.315
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_INTERNAL_15_GC_PERCENT=56.522
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=1.71
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=45.13
PRIMER_RIGHT_15_HAIRPIN_TH=41.74
PRIMER_INTERNAL_15_HAIRPIN_TH=41.60
PRIMER_LEFT_15_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_15_END_STABILITY=3.3200
PRIMER_RIGHT_15_END_STABILITY=3.6900
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=13.2273
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_16_PENALTY=1.475039
PRIMER_RIGHT_16_PENALTY=1.914379
PRIMER_INTERNAL_16_PENALTY=3.807618
PRIMER_LEFT_16_SEQUENCE=CCATTCGATCCCGACCCAA
PRIMER_RIGHT_16_SEQUENCE=GCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_16=69,19
PRIMER_RIGHT_16=96,19
PRIMER_INTERNAL_16=62,22
PRIMER_LEFT_16_TM=59.475
PRIMER_RIGHT_16_TM=58.086
PRIMER_INTERNAL_16_TM=58.192
PRIMER_LEFT_16_GC_PERCENT=57.895
PRIMER_RIGHT_16_GC_PERCENT=57.895
PRIMER_INTERNAL_16_GC_PERCENT=59.091
PRIMER_INTERNAL_16_SELF_ANY_TH=7.03
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=1.71
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=45.50
PRIMER_RIGHT_16_HAIRPIN_TH=41.74
PRIMER_INTERNAL_16_HAIRPIN_TH=41.60
PRIMER_LEFT_16_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_16_END_STABILITY=4.1200
PRIMER_RIGHT_16_END_STABILITY=5.1400
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_17_PENALTY=1.559940
PRIMER_RIGHT_17_PENALTY=1.926077
PRIMER_INTERNAL_17_PENALTY=3.826170
PRIMER_LEFT_17_SEQUENCE=CGATCCCGACCCAAATCGA
PRIMER_RIGHT_17_SEQUENCE=GGGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA
PRIMER_LEFT_17=74,19
PRIMER_RIGHT_17=80,21
PRIMER_INTERNAL_17=68,21
PRIMER_LEFT_17_TM=59.560
PRIMER_RIGHT_17_TM=59.926
PRIMER_INTERNAL_17_TM=57.174
PRIMER_LEFT_17_GC_PERCENT=57.895
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=45.13
PRIMER_RIGHT_17_HAIRPIN_TH=41.64
PRIMER_INTERNAL_17_HAIRPIN_TH=41.60
PRIMER_LEFT_17_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_17_END_STABILITY=3.5900
PRIMER_RIGHT_17_END_STABILITY=1.7300
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=12.3668
PRIMER_LEFT_18_PENALTY=1.559940
PRIMER_RIGHT_18_PENALTY=1.960819
PRIMER_INTERNAL_18_PENALTY=3.864865
PRIMER_LEFT_18_SEQUENCE=TCGATCCCGACCCAAATCG
PRIMER_RIGHT_18_SEQUENCE=CTATCGATTTGGGTCGGGATC
PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT
PRIMER_LEFT_18=73,19
PRIMER_RIGHT_18=95,21
PRIMER_INTERNAL_18=37,23
PRIMER_LEFT_18_TM=59.560
PRIMER_RIGHT_18_TM=58.039
PRIMER_INTERNAL_18_TM=59.135
PRIMER_LEFT_18_GC_PERCENT=57.895
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_INTERNAL_18_GC_PERCENT=52.174
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=1.71
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=40.30
PRIMER_RIGHT_18_HAIRPIN_TH=41.74
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_18_END_STABILITY=3.3400
PRIMER_RIGHT_18_END_STABILITY=3.3600
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=0.0363
PRIMER_LEFT_19_PENALTY=1.568623
PRIMER_RIGHT_19_PENALTY=1.997913
PRIMER_INTERNAL_19_PENALTY=3.881423
PRIMER_LEFT_19_SEQUENCE=ATTAGTGGGCCATTCGATCC
PRIMER_RIGHT_19_SEQUENCE=GCTATCGATTTGGGTCGGGAT
PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_19=60,20
PRIMER_RIGHT_19=96,21
PRIMER_INTERNAL_19=62,21
PRIMER_LEFT_19_TM=57.431
PRIMER_RIGHT_19_TM=59.998
PRIMER_INTERNAL_19_TM=57.119
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_INTERNAL_19_GC_PERCENT=57.143
PRIMER_INTERNAL_19_SELF_ANY_TH=7.03
PRIMER_LEFT_19_SELF_ANY_TH=10.70
PRIMER_RIGHT_19_SELF_ANY_TH=1.71
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=41.74
PRIMER_INTERNAL_19_HAIRPIN_TH=39.49
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_19_END_STABILITY=3.3600
PRIMER_RIGHT_19_END_STABILITY=3.8500
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=9.4710
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=1.673833
PRIMER_RIGHT_20_PENALTY=2.038681
PRIMER_INTERNAL_20_PENALTY=3.936624
PRIMER_LEFT_20_SEQUENCE=CCGACCCAAATCGATAGCTAC
PRIMER_RIGHT_20_SEQUENCE=TCGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA
PRIMER_LEFT_20=79,21
PRIMER_RIGHT_20=92,20
PRIMER_INTERNAL_20=65,22
PRIMER_LEFT_20_TM=58.326
PRIMER_RIGHT_20_TM=61.039
PRIMER_INTERNAL_20_TM=61.937
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_INTERNAL_20_GC_PERCENT=63.636
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=3.47
PRIMER_INTERNAL_20_SELF_END_TH=3.99
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=3.47
PRIMER_LEFT_20_HAIRPIN_TH=45.13
PRIMER_RIGHT_20_HAIRPIN_TH=45.48
PRIMER_INTERNAL_20_HAIRPIN_TH=42.67
PRIMER_LEFT_20_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_20_END_STABILITY=3.5800
PRIMER_RIGHT_20_END_STABILITY=3.5900
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.3824
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_21_PENALTY=1.726673
PRIMER_RIGHT_21_PENALTY=2.150848
PRIMER_INTERNAL_21_PENALTY=3.956741
PRIMER_LEFT_21_SEQUENCE=AGACATGACACTGACGATGCA
PRIMER_RIGHT_21_SEQUENCE=GCATCGTCAGTGTCATGTCTA
PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_LEFT_21=10,21
PRIMER_RIGHT_21=29,21
PRIMER_INTERNAL_21=67,19
PRIMER_LEFT_21_TM=59.727
PRIMER_RIGHT_21_TM=57.849
PRIMER_INTERNAL_21_TM=57.043
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_INTERNAL_21_GC_PERCENT=68.421
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.60
PRIMER_LEFT_21_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_21_END_STABILITY=3.9600
PRIMER_RIGHT_21_END_STABILITY=2.5900
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=9.1785
PRIMER_LEFT_22_PENALTY=1.728621
PRIMER_RIGHT_22_PENALTY=2.496898
PRIMER_INTERNAL_22_PENALTY=3.956741
PRIMER_LEFT_22_SEQUENCE=CGATCCCGACCCAAATCGATA
PRIMER_RIGHT_22_SEQUENCE=GTCTGCATCGTCAGTGTCA
PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_LEFT_22=74,21
PRIMER_RIGHT_22=33,19
PRIMER_INTERNAL_22=66,19
PRIMER_LEFT_22_TM=59.729
PRIMER_RIGHT_22_TM=57.503
PRIMER_INTERNAL_22_TM=57.043
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=52.632
PRIMER_INTERNAL_22_GC_PERCENT=68.421
PRIMER_INTERNAL_22_SELF_ANY_TH=2.18
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=45.13
PRIMER_RIGHT_22_HAIRPIN_TH=35.63
PRIMER_INTERNAL_22_HAIRPIN_TH=41.60
PRIMER_LEFT_22_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_22_END_STABILITY=2.9200
PRIMER_RIGHT_22_END_STABILITY=3.5800
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=6.6045
PRIMER_LEFT_23_PENALTY=1.914379
PRIMER_RIGHT_23_PENALTY=2.637998
PRIMER_INTERNAL_23_PENALTY=4.001118
PRIMER_LEFT_23_SEQUENCE=CCCGACCCAAATCGATAGC
PRIMER_RIGHT_23_SEQUENCE=TCGTAGCTATCGATTTGGGTC
PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT
PRIMER_LEFT_23=78,19
PRIMER_RIGHT_23=101,21
PRIMER_INTERNAL_23=36,24
PRIMER_LEFT_23_TM=58.086
PRIMER_RIGHT_23_TM=57.362
PRIMER_INTERNAL_23_TM=60.001
PRIMER_LEFT_23_GC_PERCENT=57.895
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_INTERNAL_23_GC_PERCENT=54.167
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=1.71
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=45.13
PRIMER_RIGHT_23_HAIRPIN_TH=32.30
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_23_END_STABILITY=2.9700
PRIMER_RIGHT_23_END_STABILITY=4.4600
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=3.1819
PRIMER_LEFT_24_PENALTY=1.926077
PRIMER_RIGHT_24_PENALTY=2.695830
PRIMER_INTERNAL_24_PENALTY=4.001118
PRIMER_LEFT_24_SEQUENCE=ATTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_24_SEQUENCE=GGATCGAATGGCCCACTAATA
PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG
PRIMER_LEFT_24=60,21
PRIMER_RIGHT_24=79,21
PRIMER_INTERNAL_24=35,24
PRIMER_LEFT_24_TM=59.926
PRIMER_RIGHT_24_TM=57.304
PRIMER_INTERNAL_24_TM=60.001
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_INTERNAL_24_GC_PERCENT=54.167
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=10.70
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=40.47
PRIMER_RIGHT_24_HAIRPIN_TH=0.00
PRIMER_INTERNAL_24_HAIRPIN_TH=0.00
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_24_END_STABILITY=3.8500
PRIMER_RIGHT_24_END_STABILITY=0.9800
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.5023
PRIMER_LEFT_25_PENALTY=1.960819
PRIMER_RIGHT_25_PENALTY=2.754380
PRIMER_INTERNAL_25_PENALTY=4.058186
PRIMER_LEFT_25_SEQUENCE=GATCCCGACCCAAATCGATAG
PRIMER_RIGHT_25_SEQUENCE=CGGGATCGAATGGCCCACTA
PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC
PRIMER_LEFT_25=75,21
PRIMER_RIGHT_25=81,20
PRIMER_INTERNAL_25=67,24
PRIMER_LEFT_25_TM=58.039
PRIMER_RIGHT_25_TM=61.754
PRIMER_INTERNAL_25_TM=59.942
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=60.000
PRIMER_INTERNAL_25_GC_PERCENT=58.333
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=45.13
PRIMER_RIGHT_25_HAIRPIN_TH=42.14
PRIMER_INTERNAL_25_HAIRPIN_TH=41.60
PRIMER_LEFT_25_LIBRARY_MISPRIMING=10.00, MSTa consensus sequence
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_25_END_STABILITY=2.0800
PRIMER_RIGHT_25_END_STABILITY=2.7400
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=9.7973
PRIMER_LEFT_26_PENALTY=1.997913
PRIMER_RIGHT_26_PENALTY=2.831715
PRIMER_INTERNAL_26_PENALTY=4.099823
PRIMER_LEFT_26_SEQUENCE=ATCCCGACCCAAATCGATAGC
PRIMER_RIGHT_26_SEQUENCE=TCGTCAGTGTCATGTCTACTG
PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_26=76,21
PRIMER_RIGHT_26=26,21
PRIMER_INTERNAL_26=66,23
PRIMER_LEFT_26_TM=59.998
PRIMER_RIGHT_26_TM=57.168
PRIMER_INTERNAL_26_TM=61.100
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=60.870
PRIMER_INTERNAL_26_SELF_ANY_TH=2.18
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=15.66
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=15.66
PRIMER_LEFT_26_HAIRPIN_TH=45.13
PRIMER_RIGHT_26_HAIRPIN_TH=42.34
PRIMER_INTERNAL_26_HAIRPIN_TH=41.60
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=2.9700
PRIMER_RIGHT_26_END_STABILITY=2.7400
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=24.9329
PRIMER_LEFT_27_PENALTY=2.038681
PRIMER_RIGHT_27_PENALTY=2.831715
PRIMER_INTERNAL_27_PENALTY=4.159384
PRIMER_LEFT_27_SEQUENCE=TCGATCCCGACCCAAATCGA
PRIMER_RIGHT_27_SEQUENCE=CGTCAGTGTCATGTCTACTGA
PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT
PRIMER_LEFT_27=73,20
PRIMER_RIGHT_27=25,21
PRIMER_INTERNAL_27=38,22
PRIMER_LEFT_27_TM=61.039
PRIMER_RIGHT_27_TM=57.168
PRIMER_INTERNAL_27_TM=57.841
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=54.545
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=2.47
PRIMER_RIGHT_27_SELF_ANY_TH=18.56
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=2.47
PRIMER_RIGHT_27_SELF_END_TH=18.56
PRIMER_LEFT_27_HAIRPIN_TH=45.41
PRIMER_RIGHT_27_HAIRPIN_TH=46.05
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER44C Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=3.5900
PRIMER_RIGHT_27_END_STABILITY=3.4100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=26.0802
PRIMER_LEFT_28_PENALTY=2.150848
PRIMER_RIGHT_28_PENALTY=2.912899
PRIMER_INTERNAL_28_PENALTY=4.159384
PRIMER_LEFT_28_SEQUENCE=TAGACATGACACTGACGATGC
PRIMER_RIGHT_28_SEQUENCE=TGCATCGTCAGTGTCATGT
PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG
PRIMER_LEFT_28=9,21
PRIMER_RIGHT_28=30,19
PRIMER_INTERNAL_28=37,22
PRIMER_LEFT_28_TM=57.849
PRIMER_RIGHT_28_TM=57.087
PRIMER_INTERNAL_28_TM=57.841
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.368
PRIMER_INTERNAL_28_GC_PERCENT=54.545
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=0.00
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_28_END_STABILITY=3.9100
PRIMER_RIGHT_28_END_STABILITY=3.2100
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=3.7339
PRIMER_LEFT_29_PENALTY=2.469564
PRIMER_RIGHT_29_PENALTY=2.989104
PRIMER_INTERNAL_29_PENALTY=4.196497
PRIMER_LEFT_29_SEQUENCE=CATTCGATCCCGACCCAAA
PRIMER_RIGHT_29_SEQUENCE=AGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG
PRIMER_LEFT_29=70,19
PRIMER_RIGHT_29=97,19
PRIMER_INTERNAL_29=36,23
PRIMER_LEFT_29_TM=57.530
PRIMER_RIGHT_29_TM=57.011
PRIMER_INTERNAL_29_TM=58.804
PRIMER_LEFT_29_GC_PERCENT=52.632
PRIMER_RIGHT_29_GC_PERCENT=52.632
PRIMER_INTERNAL_29_GC_PERCENT=56.522
PRIMER_INTERNAL_29_SELF_ANY_TH=0.00
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=1.71
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=45.50
PRIMER_RIGHT_29_HAIRPIN_TH=41.74
PRIMER_INTERNAL_29_HAIRPIN_TH=0.00
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_29_END_STABILITY=3.2800
PRIMER_RIGHT_29_END_STABILITY=4.7900
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=2.2809
PRIMER_LEFT_30_PENALTY=2.476833
PRIMER_RIGHT_30_PENALTY=3.032680
PRIMER_INTERNAL_30_PENALTY=4.232604
PRIMER_LEFT_30_SEQUENCE=CCATTCGATCCCGACCCAAAT
PRIMER_RIGHT_30_SEQUENCE=AGCTATCGATTTGGGTCGGGA
PRIMER_INTERNAL_30_SEQUENCE=GCCATTCGATCCCGACCCAAATCG
PRIMER_LEFT_30=69,21
PRIMER_RIGHT_30=97,21
PRIMER_INTERNAL_30=68,24
PRIMER_LEFT_30_TM=60.477
PRIMER_RIGHT_30_TM=61.033
PRIMER_INTERNAL_30_TM=60.233
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_INTERNAL_30_GC_PERCENT=58.333
PRIMER_INTERNAL_30_SELF_ANY_TH=0.00
PRIMER_LEFT_30_SELF_ANY_TH=0.00
PRIMER_RIGHT_30_SELF_ANY_TH=1.71
PRIMER_INTERNAL_30_SELF_END_TH=0.00
PRIMER_LEFT_30_SELF_END_TH=0.00
PRIMER_RIGHT_30_SELF_END_TH=0.00
PRIMER_LEFT_30_HAIRPIN_TH=45.50
PRIMER_RIGHT_30_HAIRPIN_TH=41.74
PRIMER_INTERNAL_30_HAIRPIN_TH=41.60
PRIMER_LEFT_30_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_30_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_30_END_STABILITY=2.3200
PRIMER_RIGHT_30_END_STABILITY=5.1400
PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=2.4428
PRIMER_LEFT_31_PENALTY=2.496898
PRIMER_RIGHT_31_PENALTY=4.101796
PRIMER_INTERNAL_31_PENALTY=4.351864
PRIMER_LEFT_31_SEQUENCE=TGACACTGACGATGCAGAC
PRIMER_RIGHT_31_SEQUENCE=TCGGGATCGAATGGCCCAC
PRIMER_INTERNAL_31_SEQUENCE=TTAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_31=15,19
PRIMER_RIGHT_31=82,19
PRIMER_INTERNAL_31=61,24
PRIMER_LEFT_31_TM=57.503
PRIMER_RIGHT_31_TM=62.102
PRIMER_INTERNAL_31_TM=60.352
PRIMER_LEFT_31_GC_PERCENT=52.632
PRIMER_RIGHT_31_GC_PERCENT=63.158
PRIMER_INTERNAL_31_GC_PERCENT=58.333
PRIMER_INTERNAL_31_SELF_ANY_TH=7.03
PRIMER_LEFT_31_SELF_ANY_TH=10.65
PRIMER_RIGHT_31_SELF_ANY_TH=0.00
PRIMER_INTERNAL_31_SELF_END_TH=0.00
PRIMER_LEFT_31_SELF_END_TH=0.00
PRIMER_RIGHT_31_SELF_END_TH=0.00
PRIMER_LEFT_31_HAIRPIN_TH=39.20
PRIMER_RIGHT_31_HAIRPIN_TH=43.03
PRIMER_INTERNAL_31_HAIRPIN_TH=41.60
PRIMER_LEFT_31_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_31_END_STABILITY=3.5100
PRIMER_RIGHT_31_END_STABILITY=4.6100
PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=16.1639
PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=11.0013
PRIMER_LEFT_32_PENALTY=2.540363
PRIMER_RIGHT_32_PENALTY=4.106986
PRIMER_INTERNAL_32_PENALTY=4.478673
PRIMER_LEFT_32_SEQUENCE=ATTCGATCCCGACCCAAATCG
PRIMER_RIGHT_32_SEQUENCE=CGGGATCGAATGGCCCACT
PRIMER_INTERNAL_32_SEQUENCE=TTAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_32=71,21
PRIMER_RIGHT_32=81,19
PRIMER_INTERNAL_32=61,23
PRIMER_LEFT_32_TM=60.540
PRIMER_RIGHT_32_TM=62.107
PRIMER_INTERNAL_32_TM=58.521
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=63.158
PRIMER_INTERNAL_32_GC_PERCENT=56.522
PRIMER_INTERNAL_32_SELF_ANY_TH=7.03
PRIMER_LEFT_32_SELF_ANY_TH=0.00
PRIMER_RIGHT_32_SELF_ANY_TH=0.00
PRIMER_INTERNAL_32_SELF_END_TH=0.00
PRIMER_LEFT_32_SELF_END_TH=0.00
PRIMER_RIGHT_32_SELF_END_TH=0.00
PRIMER_LEFT_32_HAIRPIN_TH=45.50
PRIMER_RIGHT_32_HAIRPIN_TH=42.14
PRIMER_INTERNAL_32_HAIRPIN_TH=41.60
PRIMER_LEFT_32_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_32_END_STABILITY=3.3400
PRIMER_RIGHT_32_END_STABILITY=4.0000
PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=2.4855
PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=10.5305
PRIMER_LEFT_33_PENALTY=2.637998
PRIMER_RIGHT_33_PENALTY=4.250296
PRIMER_INTERNAL_33_PENALTY=4.484274
PRIMER_LEFT_33_SEQUENCE=GACCCAAATCGATAGCTACGA
PRIMER_RIGHT_33_SEQUENCE=CGGGATCGAATGGCCCACTAA
PRIMER_INTERNAL_33_SEQUENCE=TTAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_33=81,21
PRIMER_RIGHT_33=81,21
PRIMER_INTERNAL_33=61,22
PRIMER_LEFT_33_TM=57.362
PRIMER_RIGHT_33_TM=62.250
PRIMER_INTERNAL_33_TM=57.516
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=57.143
PRIMER_INTERNAL_33_GC_PERCENT=54.545
PRIMER_INTERNAL_33_SELF_ANY_TH=7.03
PRIMER_LEFT_33_SELF_ANY_TH=16.85
PRIMER_RIGHT_33_SELF_ANY_TH=0.00
PRIMER_INTERNAL_33_SELF_END_TH=0.00
PRIMER_LEFT_33_SELF_END_TH=16.85
PRIMER_RIGHT_33_SELF_END_TH=0.00
PRIMER_LEFT_33_HAIRPIN_TH=43.65
PRIMER_RIGHT_33_HAIRPIN_TH=42.14
PRIMER_INTERNAL_33_HAIRPIN_TH=39.49
PRIMER_LEFT_33_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_RIGHT_33_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_33_END_STABILITY=3.4300
PRIMER_RIGHT_33_END_STABILITY=2.2400
PRIMER_LEFT_33_TEMPLATE_MISPRIMING_TH=20.0470
PRIMER_RIGHT_33_TEMPLATE_MISPRIMING_TH=10.9108
PRIMER_LEFT_34_PENALTY=2.695830
PRIMER_INTERNAL_34_PENALTY=4.552303
PRIMER_LEFT_34_SEQUENCE=TATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_34_SEQUENCE=ACACACACACACACAGCACACA
PRIMER_LEFT_34=59,21
PRIMER_INTERNAL_34=35,22
PRIMER_LEFT_34_TM=57.304
PRIMER_INTERNAL_34_TM=57.448
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_INTERNAL_34_GC_PERCENT=50.000
PRIMER_INTERNAL_34_SELF_ANY_TH=0.00
PRIMER_LEFT_34_SELF_ANY_TH=10.70
PRIMER_INTERNAL_34_SELF_END_TH=0.00
PRIMER_LEFT_34_SELF_END_TH=0.00
PRIMER_LEFT_34_HAIRPIN_TH=0.00
PRIMER_INTERNAL_34_HAIRPIN_TH=0.00
PRIMER_LEFT_34_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_34_END_STABILITY=3.3600
PRIMER_LEFT_34_TEMPLATE_MISPRIMING_TH=9.4710
PRIMER_LEFT_35_PENALTY=2.754380
PRIMER_INTERNAL_35_PENALTY=4.639434
PRIMER_LEFT_35_SEQUENCE=TAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_35_SEQUENCE=GCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_35=62,20
PRIMER_INTERNAL_35=68,22
PRIMER_LEFT_35_TM=61.754
PRIMER_INTERNAL_35_TM=57.361
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_INTERNAL_35_GC_PERCENT=54.545
PRIMER_INTERNAL_35_SELF_ANY_TH=0.00
PRIMER_LEFT_35_SELF_ANY_TH=10.70
PRIMER_INTERNAL_35_SELF_END_TH=0.00
PRIMER_LEFT_35_SELF_END_TH=0.00
PRIMER_LEFT_35_HAIRPIN_TH=42.98
PRIMER_INTERNAL_35_HAIRPIN_TH=41.60
PRIMER_LEFT_35_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_35_END_STABILITY=5.1400
PRIMER_LEFT_35_TEMPLATE_MISPRIMING_TH=6.4478
PRIMER_LEFT_36_PENALTY=2.772619
PRIMER_INTERNAL_36_PENALTY=4.809017
PRIMER_LEFT_36_SEQUENCE=TTCGATCCCGACCCAAATC
PRIMER_INTERNAL_36_SEQUENCE=ACACACACACACACAGCACACAG
PRIMER_LEFT_36=72,19
PRIMER_INTERNAL_36=35,23
PRIMER_LEFT_36_TM=57.227
PRIMER_INTERNAL_36_TM=58.191
PRIMER_LEFT_36_GC_PERCENT=52.632
PRIMER_INTERNAL_36_GC_PERCENT=52.174
PRIMER_INTERNAL_36_SELF_ANY_TH=0.00
PRIMER_LEFT_36_SELF_ANY_TH=0.00
PRIMER_INTERNAL_36_SELF_END_TH=0.00
PRIMER_LEFT_36_SELF_END_TH=0.00
PRIMER_LEFT_36_HAIRPIN_TH=45.50
PRIMER_INTERNAL_36_HAIRPIN_TH=0.00
PRIMER_LEFT_36_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_36_END_STABILITY=2.1700
PRIMER_LEFT_36_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_37_PENALTY=2.831715
PRIMER_INTERNAL_37_PENALTY=4.881095
PRIMER_LEFT_37_SEQUENCE=CAGTAGACATGACACTGACGA
PRIMER_INTERNAL_37_SEQUENCE=GCCATTCGATCCCGACCCAAATC
PRIMER_LEFT_37=6,21
PRIMER_INTERNAL_37=68,23
PRIMER_LEFT_37_TM=57.168
PRIMER_INTERNAL_37_TM=58.119
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_INTERNAL_37_GC_PERCENT=56.522
PRIMER_INTERNAL_37_SELF_ANY_TH=0.00
PRIMER_LEFT_37_SELF_ANY_TH=16.10
PRIMER_INTERNAL_37_SELF_END_TH=0.00
PRIMER_LEFT_37_SELF_END_TH=0.00
PRIMER_LEFT_37_HAIRPIN_TH=41.80
PRIMER_INTERNAL_37_HAIRPIN_TH=41.60
PRIMER_LEFT_37_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_37_END_STABILITY=4.2000
PRIMER_LEFT_37_TEMPLATE_MISPRIMING_TH=20.3033
PRIMER_LEFT_38_PENALTY=2.831715
PRIMER_INTERNAL_38_PENALTY=5.108025
PRIMER_LEFT_38_SEQUENCE=TCAGTAGACATGACACTGACG
PRIMER_INTERNAL_38_SEQUENCE=TGGGCCATTCGATCCCGACCCAA
PRIMER_LEFT_38=5,21
PRIMER_INTERNAL_38=65,23
PRIMER_LEFT_38_TM=57.168
PRIMER_INTERNAL_38_TM=62.108
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_INTERNAL_38_GC_PERCENT=60.870
PRIMER_INTERNAL_38_SELF_ANY_TH=0.00
PRIMER_LEFT_38_SELF_ANY_TH=18.35
PRIMER_INTERNAL_38_SELF_END_TH=0.00
PRIMER_LEFT_38_SELF_END_TH=0.00
PRIMER_LEFT_38_HAIRPIN_TH=42.75
PRIMER_INTERNAL_38_HAIRPIN_TH=41.29
PRIMER_LEFT_38_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_38_END_STABILITY=4.3500
PRIMER_LEFT_38_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_39_PENALTY=2.912899
PRIMER_INTERNAL_39_PENALTY=5.111368
PRIMER_LEFT_39_SEQUENCE=ACATGACACTGACGATGCA
PRIMER_INTERNAL_39_SEQUENCE=CCATTCGATCCCGACCCAAATCGA
PRIMER_LEFT_39=12,19
PRIMER_INTERNAL_39=69,24
PRIMER_LEFT_39_TM=57.087
PRIMER_INTERNAL_39_TM=58.889
PRIMER_LEFT_39_GC_PERCENT=47.368
PRIMER_INTERNAL_39_GC_PERCENT=54.167
PRIMER_INTERNAL_39_SELF_ANY_TH=0.00
PRIMER_LEFT_39_SELF_ANY_TH=0.00
PRIMER_INTERNAL_39_SELF_END_TH=0.00
PRIMER_LEFT_39_SELF_END_TH=0.00
PRIMER_LEFT_39_HAIRPIN_TH=0.00
PRIMER_INTERNAL_39_HAIRPIN_TH=42.01
PRIMER_LEFT_39_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_39_END_STABILITY=3.9600
PRIMER_LEFT_39_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_40_PENALTY=2.989104
PRIMER_INTERNAL_40_PENALTY=5.111455
PRIMER_LEFT_40_SEQUENCE=CCGACCCAAATCGATAGCT
PRIMER_INTERNAL_40_SEQUENCE=CCATTCGATCCCGACCCAAATCG
PRIMER_LEFT_40=79,19
PRIMER_INTERNAL_40=69,23
PRIMER_LEFT_40_TM=57.011
PRIMER_INTERNAL_40_TM=57.889
PRIMER_LEFT_40_GC_PERCENT=52.632
PRIMER_INTERNAL_40_GC_PERCENT=56.522
PRIMER_INTERNAL_40_SELF_ANY_TH=0.00
PRIMER_LEFT_40_SELF_ANY_TH=0.00
PRIMER_INTERNAL_40_SELF_END_TH=0.00
PRIMER_LEFT_40_SELF_END_TH=0.00
PRIMER_LEFT_40_HAIRPIN_TH=45.13
PRIMER_INTERNAL_40_HAIRPIN_TH=41.60
PRIMER_LEFT_40_LIBRARY_MISPRIMING=9.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_LEFT_40_END_STABILITY=3.3200
PRIMER_LEFT_40_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_LEFT_41_PENALTY=3.032680
PRIMER_INTERNAL_41_PENALTY=5.122599
PRIMER_LEFT_41_SEQUENCE=TCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_41_SEQUENCE=GGGCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_41=77,21
PRIMER_INTERNAL_41=66,24
PRIMER_LEFT_41_TM=61.033
PRIMER_INTERNAL_41_TM=61.123
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_INTERNAL_41_GC_PERCENT=58.333
PRIMER_INTERNAL_41_SELF_ANY_TH=2.18
PRIMER_LEFT_41_SELF_ANY_TH=0.00
PRIMER_INTERNAL_41_SELF_END_TH=0.00
PRIMER_LEFT_41_SELF_END_TH=0.00
PRIMER_LEFT_41_HAIRPIN_TH=45.13
PRIMER_INTERNAL_41_HAIRPIN_TH=41.60
PRIMER_LEFT_41_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_41_END_STABILITY=3.3200
PRIMER_LEFT_41_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_LEFT_42_PENALTY=3.367844
PRIMER_INTERNAL_42_PENALTY=5.295331
PRIMER_LEFT_42_SEQUENCE=TCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_42_SEQUENCE=ACACACACACACAGCACACAGGTA
PRIMER_LEFT_42=73,21
PRIMER_INTERNAL_42=37,24
PRIMER_LEFT_42_TM=61.368
PRIMER_INTERNAL_42_TM=58.705
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_INTERNAL_42_GC_PERCENT=50.000
PRIMER_INTERNAL_42_SELF_ANY_TH=0.00
PRIMER_LEFT_42_SELF_ANY_TH=0.00
PRIMER_INTERNAL_42_SELF_END_TH=0.00
PRIMER_LEFT_42_SELF_END_TH=0.00
PRIMER_LEFT_42_HAIRPIN_TH=43.48
PRIMER_INTERNAL_42_HAIRPIN_TH=0.00
PRIMER_LEFT_42_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_42_END_STABILITY=3.5900
PRIMER_LEFT_42_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_43_PENALTY=3.568322
PRIMER_INTERNAL_43_PENALTY=5.316271
PRIMER_LEFT_43_SEQUENCE=TTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_43_SEQUENCE=ATTAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_43=72,21
PRIMER_INTERNAL_43=60,23
PRIMER_LEFT_43_TM=61.568
PRIMER_INTERNAL_43_TM=57.684
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_INTERNAL_43_GC_PERCENT=52.174
PRIMER_INTERNAL_43_SELF_ANY_TH=7.03
PRIMER_LEFT_43_SELF_ANY_TH=2.47
PRIMER_INTERNAL_43_SELF_END_TH=0.00
PRIMER_LEFT_43_SELF_END_TH=2.47
PRIMER_LEFT_43_HAIRPIN_TH=45.78
PRIMER_INTERNAL_43_HAIRPIN_TH=39.49
PRIMER_LEFT_43_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_43_END_STABILITY=3.5900
PRIMER_LEFT_43_TEMPLATE_MISPRIMING_TH=4.8294
PRIMER_LEFT_44_PENALTY=3.602746
PRIMER_INTERNAL_44_PENALTY=5.357946
PRIMER_LEFT_44_SEQUENCE=GCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_44_SEQUENCE=ATTAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_44=68,20
PRIMER_INTERNAL_44=60,24
PRIMER_LEFT_44_TM=62.603
PRIMER_INTERNAL_44_TM=58.642
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_INTERNAL_44_GC_PERCENT=54.167
PRIMER_INTERNAL_44_SELF_ANY_TH=7.03
PRIMER_LEFT_44_SELF_ANY_TH=0.00
PRIMER_INTERNAL_44_SELF_END_TH=0.00
PRIMER_LEFT_44_SELF_END_TH=0.00
PRIMER_LEFT_44_HAIRPIN_TH=45.50
PRIMER_INTERNAL_44_HAIRPIN_TH=41.60
PRIMER_LEFT_44_LIBRARY_MISPRIMING=10.00, MER4A a consensus
PRIMER_LEFT_44_END_STABILITY=4.1200
PRIMER_LEFT_44_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_45_PENALTY=4.101796
PRIMER_INTERNAL_45_PENALTY=5.401146
PRIMER_LEFT_45_SEQUENCE=GCCATTCGATCCCGACCCA
PRIMER_INTERNAL_45_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_45=68,19
PRIMER_INTERNAL_45=60,25
PRIMER_LEFT_45_TM=62.102
PRIMER_INTERNAL_45_TM=60.401
PRIMER_LEFT_45_GC_PERCENT=63.158
PRIMER_INTERNAL_45_GC_PERCENT=56.000
PRIMER_INTERNAL_45_SELF_ANY_TH=7.03
PRIMER_LEFT_45_SELF_ANY_TH=0.00
PRIMER_INTERNAL_45_SELF_END_TH=0.00
PRIMER_LEFT_45_SELF_END_TH=0.00
PRIMER_LEFT_45_HAIRPIN_TH=45.50
PRIMER_INTERNAL_45_HAIRPIN_TH=41.60
PRIMER_LEFT_45_LIBRARY_MISPRIMING=10.00, TCCCA Primate simple repeat pattern
PRIMER_LEFT_45_END_STABILITY=4.5100
PRIMER_LEFT_45_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_46_PENALTY=4.101796
PRIMER_INTERNAL_46_PENALTY=5.439697
PRIMER_LEFT_46_SEQUENCE=TGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_46_SEQUENCE=CACACACACACACAGCACACAGGTA
PRIMER_LEFT_46=65,19
PRIMER_INTERNAL_46=36,25
PRIMER_LEFT_46_TM=62.102
PRIMER_INTERNAL_46_TM=59.560
PRIMER_LEFT_46_GC_PERCENT=63.158
PRIMER_INTERNAL_46_GC_PERCENT=52.000
PRIMER_INTERNAL_46_SELF_ANY_TH=0.00
PRIMER_LEFT_46_SELF_ANY_TH=0.00
PRIMER_INTERNAL_46_SELF_END_TH=0.00
PRIMER_LEFT_46_SELF_END_TH=0.00
PRIMER_LEFT_46_HAIRPIN_TH=45.50
PRIMER_INTERNAL_46_HAIRPIN_TH=0.00
PRIMER_LEFT_46_LIBRARY_MISPRIMING=9.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_46_END_STABILITY=4.7900
PRIMER_LEFT_46_TEMPLATE_MISPRIMING_TH=4.9124
PRIMER_LEFT_47_PENALTY=4.101796
PRIMER_INTERNAL_47_PENALTY=5.459011
PRIMER_LEFT_47_SEQUENCE=GTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_47_SEQUENCE=AGTGGGCCATTCGATCCCGACCC
PRIMER_LEFT_47=64,19
PRIMER_INTERNAL_47=63,23
PRIMER_LEFT_47_TM=62.102
PRIMER_INTERNAL_47_TM=62.459
PRIMER_LEFT_47_GC_PERCENT=63.158
PRIMER_INTERNAL_47_GC_PERCENT=65.217
PRIMER_INTERNAL_47_SELF_ANY_TH=7.03
PRIMER_LEFT_47_SELF_ANY_TH=10.70
PRIMER_INTERNAL_47_SELF_END_TH=0.00
PRIMER_LEFT_47_SELF_END_TH=0.00
PRIMER_LEFT_47_HAIRPIN_TH=42.98
PRIMER_INTERNAL_47_HAIRPIN_TH=41.60
PRIMER_LEFT_47_LIBRARY_MISPRIMING=9.00, MER1B Nonautonomous DNA transposon
PRIMER_LEFT_47_END_STABILITY=5.1400
PRIMER_LEFT_47_TEMPLATE_MISPRIMING_TH=10.7677
PRIMER_LEFT_48_PENALTY=4.106986
PRIMER_INTERNAL_48_PENALTY=5.514983
PRIMER_LEFT_48_SEQUENCE=AGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_48_SEQUENCE=TCGATCCCGACCCAAATCGATAGC
PRIMER_LEFT_48=63,19
PRIMER_INTERNAL_48=73,24
PRIMER_LEFT_48_TM=62.107
PRIMER_INTERNAL_48_TM=58.485
PRIMER_LEFT_48_GC_PERCENT=63.158
PRIMER_INTERNAL_48_GC_PERCENT=54.167
PRIMER_INTERNAL_48_SELF_ANY_TH=0.00
PRIMER_LEFT_48_SELF_ANY_TH=10.70
PRIMER_INTERNAL_48_SELF_END_TH=0.00
PRIMER_LEFT_48_SELF_END_TH=0.00
PRIMER_LEFT_48_HAIRPIN_TH=42.98
PRIMER_INTERNAL_48_HAIRPIN_TH=38.51
PRIMER_LEFT_48_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_48_END_STABILITY=5.1400
PRIMER_LEFT_48_TEMPLATE_MISPRIMING_TH=6.4478
PRIMER_LEFT_49_PENALTY=4.250296
PRIMER_INTERNAL_49_PENALTY=5.518553
PRIMER_LEFT_49_SEQUENCE=TTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_49_SEQUENCE=CGATCCCGACCCAAATCGATAGCT
PRIMER_LEFT_49=61,21
PRIMER_INTERNAL_49=74,24
PRIMER_LEFT_49_TM=62.250
PRIMER_INTERNAL_49_TM=58.481
PRIMER_LEFT_49_GC_PERCENT=57.143
PRIMER_INTERNAL_49_GC_PERCENT=54.167
PRIMER_INTERNAL_49_SELF_ANY_TH=0.00
PRIMER_LEFT_49_SELF_ANY_TH=10.70
PRIMER_INTERNAL_49_SELF_END_TH=0.00
PRIMER_LEFT_49_SELF_END_TH=0.00
PRIMER_LEFT_49_HAIRPIN_TH=42.98
PRIMER_INTERNAL_49_HAIRPIN_TH=41.28
PRIMER_LEFT_49_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_49_END_STABILITY=5.1400
PRIMER_LEFT_49_TEMPLATE_MISPRIMING_TH=6.4478
PRIMER_LEFT_50_PENALTY=4.826926
PRIMER_INTERNAL_50_PENALTY=5.523565
PRIMER_LEFT_50_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_INTERNAL_50_SEQUENCE=CGATCCCGACCCAAATCGATAGC
PRIMER_LEFT_50=67,19
PRIMER_INTERNAL_50=74,23
PRIMER_LEFT_50_TM=62.827
PRIMER_INTERNAL_50_TM=57.476
PRIMER_LEFT_50_GC_PERCENT=68.421
PRIMER_INTERNAL_50_GC_PERCENT=56.522
PRIMER_INTERNAL_50_SELF_ANY_TH=0.00
PRIMER_LEFT_50_SELF_ANY_TH=0.00
PRIMER_INTERNAL_50_SELF_END_TH=0.00
PRIMER_LEFT_50_SELF_END_TH=0.00
PRIMER_LEFT_50_HAIRPIN_TH=45.50
PRIMER_INTERNAL_50_HAIRPIN_TH=41.28
PRIMER_LEFT_50_LIBRARY_MISPRIMING=9.00, TCCCA Primate simple repeat pattern
PRIMER_LEFT_50_END_STABILITY=4.4600
PRIMER_LEFT_50_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_51_PENALTY=4.826926
PRIMER_INTERNAL_51_PENALTY=5.550131
PRIMER_LEFT_51_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_INTERNAL_51_SEQUENCE=CACACACACACAGCACACAGGTA
PRIMER_LEFT_51=66,19
PRIMER_INTERNAL_51=38,23
PRIMER_LEFT_51_TM=62.827
PRIMER_INTERNAL_51_TM=57.450
PRIMER_LEFT_51_GC_PERCENT=68.421
PRIMER_INTERNAL_51_GC_PERCENT=52.174
PRIMER_INTERNAL_51_SELF_ANY_TH=0.00
PRIMER_LEFT_51_SELF_ANY_TH=4.39
PRIMER_INTERNAL_51_SELF_END_TH=0.00
PRIMER_LEFT_51_SELF_END_TH=0.00
PRIMER_LEFT_51_HAIRPIN_TH=45.50
PRIMER_INTERNAL_51_HAIRPIN_TH=0.00
PRIMER_LEFT_51_LIBRARY_MISPRIMING=9.00, HSATII Human satellite II DNA
PRIMER_LEFT_51_END_STABILITY=4.7900
PRIMER_LEFT_51_TEMPLATE_MISPRIMING_TH=3.1645
PRIMER_INTERNAL_52_PENALTY=5.564155
PRIMER_INTERNAL_52_SEQUENCE=TCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_52=73,25
PRIMER_INTERNAL_52_TM=59.436
PRIMER_INTERNAL_52_GC_PERCENT=52.000
PRIMER_INTERNAL_52_SELF_ANY_TH=0.00
PRIMER_INTERNAL_52_SELF_END_TH=0.00
PRIMER_INTERNAL_52_HAIRPIN_TH=38.51
PRIMER_INTERNAL_53_PENALTY=5.697915
PRIMER_INTERNAL_53_SEQUENCE=GTGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_53=64,23
PRIMER_INTERNAL_53_TM=62.698
PRIMER_INTERNAL_53_GC_PERCENT=65.217
PRIMER_INTERNAL_53_SELF_ANY_TH=7.03
PRIMER_INTERNAL_53_SELF_END_TH=3.99
PRIMER_INTERNAL_53_HAIRPIN_TH=42.67
PRIMER_INTERNAL_54_PENALTY=5.806699
PRIMER_INTERNAL_54_SEQUENCE=CCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_54=78,24
PRIMER_INTERNAL_54_TM=58.193
PRIMER_INTERNAL_54_GC_PERCENT=54.167
PRIMER_INTERNAL_54_SELF_ANY_TH=12.68
PRIMER_INTERNAL_54_SELF_END_TH=12.68
PRIMER_INTERNAL_54_HAIRPIN_TH=41.28
PRIMER_INTERNAL_55_PENALTY=5.806699
PRIMER_INTERNAL_55_SEQUENCE=TCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_55=77,24
PRIMER_INTERNAL_55_TM=58.193
PRIMER_INTERNAL_55_GC_PERCENT=54.167
PRIMER_INTERNAL_55_SELF_ANY_TH=0.00
PRIMER_INTERNAL_55_SELF_END_TH=0.00
PRIMER_INTERNAL_55_HAIRPIN_TH=41.28
PRIMER_INTERNAL_56_PENALTY=5.824076
PRIMER_INTERNAL_56_SEQUENCE=CCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_56=78,23
PRIMER_INTERNAL_56_TM=57.176
PRIMER_INTERNAL_56_GC_PERCENT=56.522
PRIMER_INTERNAL_56_SELF_ANY_TH=0.00
PRIMER_INTERNAL_56_SELF_END_TH=0.00
PRIMER_INTERNAL_56_HAIRPIN_TH=41.28
PRIMER_INTERNAL_57_PENALTY=5.846052
PRIMER_INTERNAL_57_SEQUENCE=TCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_57=77,25
PRIMER_INTERNAL_57_TM=59.154
PRIMER_INTERNAL_57_GC_PERCENT=52.000
PRIMER_INTERNAL_57_SELF_ANY_TH=12.68
PRIMER_INTERNAL_57_SELF_END_TH=12.68
PRIMER_INTERNAL_57_HAIRPIN_TH=41.28
PRIMER_INTERNAL_58_PENALTY=5.892980
PRIMER_INTERNAL_58_SEQUENCE=TAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_58=62,24
PRIMER_INTERNAL_58_TM=61.893
PRIMER_INTERNAL_58_GC_PERCENT=62.500
PRIMER_INTERNAL_58_SELF_ANY_TH=7.03
PRIMER_INTERNAL_58_SELF_END_TH=0.00
PRIMER_INTERNAL_58_HAIRPIN_TH=41.60
PRIMER_INTERNAL_59_PENALTY=5.951461
PRIMER_INTERNAL_59_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_59=57,25
PRIMER_INTERNAL_59_TM=59.049
PRIMER_INTERNAL_59_GC_PERCENT=56.000
PRIMER_INTERNAL_59_SELF_ANY_TH=7.03
PRIMER_INTERNAL_59_SELF_END_TH=0.00
PRIMER_INTERNAL_59_HAIRPIN_TH=39.49
PRIMER_INTERNAL_60_PENALTY=5.980536
PRIMER_INTERNAL_60_SEQUENCE=CATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_60=70,23
PRIMER_INTERNAL_60_TM=57.019
PRIMER_INTERNAL_60_GC_PERCENT=52.174
PRIMER_INTERNAL_60_SELF_ANY_TH=0.00
PRIMER_INTERNAL_60_SELF_END_TH=0.00
PRIMER_INTERNAL_60_HAIRPIN_TH=42.01
PRIMER_INTERNAL_61_PENALTY=6.009338
PRIMER_INTERNAL_61_SEQUENCE=CCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_61=69,25
PRIMER_INTERNAL_61_TM=58.991
PRIMER_INTERNAL_61_GC_PERCENT=52.000
PRIMER_INTERNAL_61_SELF_ANY_TH=2.97
PRIMER_INTERNAL_61_SELF_END_TH=0.00
PRIMER_INTERNAL_61_HAIRPIN_TH=41.68
PRIMER_INTERNAL_62_PENALTY=6.046206
PRIMER_INTERNAL_62_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_62=59,26
PRIMER_INTERNAL_62_TM=59.954
PRIMER_INTERNAL_62_GC_PERCENT=53.846
PRIMER_INTERNAL_62_SELF_ANY_TH=7.03
PRIMER_INTERNAL_62_SELF_END_TH=0.00
PRIMER_INTERNAL_62_HAIRPIN_TH=41.60
PRIMER_INTERNAL_63_PENALTY=6.046206
PRIMER_INTERNAL_63_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_63=57,26
PRIMER_INTERNAL_63_TM=59.954
PRIMER_INTERNAL_63_GC_PERCENT=53.846
PRIMER_INTERNAL_63_SELF_ANY_TH=7.03
PRIMER_INTERNAL_63_SELF_END_TH=0.00
PRIMER_INTERNAL_63_HAIRPIN_TH=39.49
PRIMER_INTERNAL_64_PENALTY=6.046659
PRIMER_INTERNAL_64_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_64=56,26
PRIMER_INTERNAL_64_TM=59.953
PRIMER_INTERNAL_64_GC_PERCENT=53.846
PRIMER_INTERNAL_64_SELF_ANY_TH=7.03
PRIMER_INTERNAL_64_SELF_END_TH=0.00
PRIMER_INTERNAL_64_HAIRPIN_TH=39.49
PRIMER_INTERNAL_65_PENALTY=6.125318
PRIMER_INTERNAL_65_SEQUENCE=GCCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_65=68,25
PRIMER_INTERNAL_65_TM=61.125
PRIMER_INTERNAL_65_GC_PERCENT=56.000
PRIMER_INTERNAL_65_SELF_ANY_TH=0.00
PRIMER_INTERNAL_65_SELF_END_TH=0.00
PRIMER_INTERNAL_65_HAIRPIN_TH=42.01
PRIMER_INTERNAL_66_PENALTY=6.131527
PRIMER_INTERNAL_66_SEQUENCE=ACACACACACACACAGCACACAGGT
PRIMER_INTERNAL_66=35,25
PRIMER_INTERNAL_66_TM=61.132
PRIMER_INTERNAL_66_GC_PERCENT=52.000
PRIMER_INTERNAL_66_SELF_ANY_TH=0.00
PRIMER_INTERNAL_66_SELF_END_TH=0.00
PRIMER_INTERNAL_66_HAIRPIN_TH=0.00
PRIMER_INTERNAL_67_PENALTY=6.186005
PRIMER_INTERNAL_67_SEQUENCE=ACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_67=37,25
PRIMER_INTERNAL_67_TM=58.814
PRIMER_INTERNAL_67_GC_PERCENT=48.000
PRIMER_INTERNAL_67_SELF_ANY_TH=0.00
PRIMER_INTERNAL_67_SELF_END_TH=0.00
PRIMER_INTERNAL_67_HAIRPIN_TH=0.00
PRIMER_INTERNAL_68_PENALTY=6.225896
PRIMER_INTERNAL_68_SEQUENCE=TTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_68=72,25
PRIMER_INTERNAL_68_TM=58.774
PRIMER_INTERNAL_68_GC_PERCENT=52.000
PRIMER_INTERNAL_68_SELF_ANY_TH=0.00
PRIMER_INTERNAL_68_SELF_END_TH=0.00
PRIMER_INTERNAL_68_HAIRPIN_TH=41.68
PRIMER_INTERNAL_69_PENALTY=6.265114
PRIMER_INTERNAL_69_SEQUENCE=TGGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_69=65,24
PRIMER_INTERNAL_69_TM=62.265
PRIMER_INTERNAL_69_GC_PERCENT=58.333
PRIMER_INTERNAL_69_SELF_ANY_TH=0.00
PRIMER_INTERNAL_69_SELF_END_TH=0.00
PRIMER_INTERNAL_69_HAIRPIN_TH=41.29
PRIMER_INTERNAL_70_PENALTY=6.318558
PRIMER_INTERNAL_70_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_70=72,26
PRIMER_INTERNAL_70_TM=59.681
PRIMER_INTERNAL_70_GC_PERCENT=50.000
PRIMER_INTERNAL_70_SELF_ANY_TH=0.00
PRIMER_INTERNAL_70_SELF_END_TH=0.00
PRIMER_INTERNAL_70_HAIRPIN_TH=41.68
PRIMER_INTERNAL_71_PENALTY=6.365515
PRIMER_INTERNAL_71_SEQUENCE=CACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_71=36,26
PRIMER_INTERNAL_71_TM=59.634
PRIMER_INTERNAL_71_GC_PERCENT=50.000
PRIMER_INTERNAL_71_SELF_ANY_TH=0.00
PRIMER_INTERNAL_71_SELF_END_TH=0.00
PRIMER_INTERNAL_71_HAIRPIN_TH=0.00
PRIMER_INTERNAL_72_PENALTY=6.390437
PRIMER_INTERNAL_72_SEQUENCE=CACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_72=38,24
PRIMER_INTERNAL_72_TM=57.610
PRIMER_INTERNAL_72_GC_PERCENT=50.000
PRIMER_INTERNAL_72_SELF_ANY_TH=0.00
PRIMER_INTERNAL_72_SELF_END_TH=0.00
PRIMER_INTERNAL_72_HAIRPIN_TH=0.00
PRIMER_INTERNAL_73_PENALTY=6.635581
PRIMER_INTERNAL_73_SEQUENCE=GGGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_73=66,25
PRIMER_INTERNAL_73_TM=61.636
PRIMER_INTERNAL_73_GC_PERCENT=60.000
PRIMER_INTERNAL_73_SELF_ANY_TH=2.18
PRIMER_INTERNAL_73_SELF_END_TH=0.00
PRIMER_INTERNAL_73_HAIRPIN_TH=41.60
PRIMER_INTERNAL_74_PENALTY=6.661393
PRIMER_INTERNAL_74_SEQUENCE=ACACACACACACACAGCACACAGGTA
PRIMER_INTERNAL_74=35,26
PRIMER_INTERNAL_74_TM=60.661
PRIMER_INTERNAL_74_GC_PERCENT=50.000
PRIMER_INTERNAL_74_SELF_ANY_TH=0.00
PRIMER_INTERNAL_74_SELF_END_TH=0.00
PRIMER_INTERNAL_74_HAIRPIN_TH=0.00
PRIMER_INTERNAL_75_PENALTY=6.679783
PRIMER_INTERNAL_75_SEQUENCE=CCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_75=78,25
PRIMER_INTERNAL_75_TM=58.320
PRIMER_INTERNAL_75_GC_PERCENT=52.000
PRIMER_INTERNAL_75_SELF_ANY_TH=14.48
PRIMER_INTERNAL_75_SELF_END_TH=14.48
PRIMER_INTERNAL_75_HAIRPIN_TH=46.54
PRIMER_INTERNAL_76_PENALTY=6.679783
PRIMER_INTERNAL_76_SEQUENCE=ATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_76=76,25
PRIMER_INTERNAL_76_TM=58.320
PRIMER_INTERNAL_76_GC_PERCENT=52.000
PRIMER_INTERNAL_76_SELF_ANY_TH=0.00
PRIMER_INTERNAL_76_SELF_END_TH=0.00
PRIMER_INTERNAL_76_HAIRPIN_TH=41.28
PRIMER_INTERNAL_77_PENALTY=6.695664
PRIMER_INTERNAL_77_SEQUENCE=TATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_77=59,24
PRIMER_INTERNAL_77_TM=57.304
PRIMER_INTERNAL_77_GC_PERCENT=50.000
PRIMER_INTERNAL_77_SELF_ANY_TH=7.03
PRIMER_INTERNAL_77_SELF_END_TH=0.00
PRIMER_INTERNAL_77_HAIRPIN_TH=39.49
PRIMER_INTERNAL_78_PENALTY=6.741797
PRIMER_INTERNAL_78_SEQUENCE=GTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_78=58,24
PRIMER_INTERNAL_78_TM=57.258
PRIMER_INTERNAL_78_GC_PERCENT=54.167
PRIMER_INTERNAL_78_SELF_ANY_TH=7.03
PRIMER_INTERNAL_78_SELF_END_TH=0.00
PRIMER_INTERNAL_78_HAIRPIN_TH=39.49
PRIMER_INTERNAL_79_PENALTY=6.754240
PRIMER_INTERNAL_79_SEQUENCE=TATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_79=59,25
PRIMER_INTERNAL_79_TM=58.246
PRIMER_INTERNAL_79_GC_PERCENT=52.000
PRIMER_INTERNAL_79_SELF_ANY_TH=7.03
PRIMER_INTERNAL_79_SELF_END_TH=0.00
PRIMER_INTERNAL_79_HAIRPIN_TH=41.60
PRIMER_INTERNAL_80_PENALTY=6.754240
PRIMER_INTERNAL_80_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_80=58,25
PRIMER_INTERNAL_80_TM=58.246
PRIMER_INTERNAL_80_GC_PERCENT=52.000
PRIMER_INTERNAL_80_SELF_ANY_TH=7.03
PRIMER_INTERNAL_80_SELF_END_TH=0.00
PRIMER_INTERNAL_80_HAIRPIN_TH=39.49
PRIMER_INTERNAL_81_PENALTY=6.756422
PRIMER_INTERNAL_81_SEQUENCE=TCCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_81=77,26
PRIMER_INTERNAL_81_TM=59.244
PRIMER_INTERNAL_81_GC_PERCENT=50.000
PRIMER_INTERNAL_81_SELF_ANY_TH=14.48
PRIMER_INTERNAL_81_SELF_END_TH=14.48
PRIMER_INTERNAL_81_HAIRPIN_TH=46.54
PRIMER_INTERNAL_82_PENALTY=6.756422
PRIMER_INTERNAL_82_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_82=76,26
PRIMER_INTERNAL_82_TM=59.244
PRIMER_INTERNAL_82_GC_PERCENT=50.000
PRIMER_INTERNAL_82_SELF_ANY_TH=12.68
PRIMER_INTERNAL_82_SELF_END_TH=12.68
PRIMER_INTERNAL_82_HAIRPIN_TH=41.28
PRIMER_INTERNAL_83_PENALTY=6.804314
PRIMER_INTERNAL_83_SEQUENCE=CATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_83=70,24
PRIMER_INTERNAL_83_TM=57.196
PRIMER_INTERNAL_83_GC_PERCENT=50.000
PRIMER_INTERNAL_83_SELF_ANY_TH=2.97
PRIMER_INTERNAL_83_SELF_END_TH=0.00
PRIMER_INTERNAL_83_HAIRPIN_TH=41.68
PRIMER_INTERNAL_84_PENALTY=6.828710
PRIMER_INTERNAL_84_SEQUENCE=GTGGGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_84=64,24
PRIMER_INTERNAL_84_TM=62.829
PRIMER_INTERNAL_84_GC_PERCENT=62.500
PRIMER_INTERNAL_84_SELF_ANY_TH=7.03
PRIMER_INTERNAL_84_SELF_END_TH=0.00
PRIMER_INTERNAL_84_HAIRPIN_TH=43.79
PRIMER_INTERNAL_85_PENALTY=6.841708
PRIMER_INTERNAL_85_SEQUENCE=CCCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_85=78,26
PRIMER_INTERNAL_85_TM=59.158
PRIMER_INTERNAL_85_GC_PERCENT=53.846
PRIMER_INTERNAL_85_SELF_ANY_TH=18.40
PRIMER_INTERNAL_85_SELF_END_TH=11.69
PRIMER_INTERNAL_85_HAIRPIN_TH=43.02
PRIMER_INTERNAL_86_PENALTY=6.895596
PRIMER_INTERNAL_86_SEQUENCE=GGCCATTCGATCCCGACCCAAATCG
PRIMER_INTERNAL_86=67,25
PRIMER_INTERNAL_86_TM=61.896
PRIMER_INTERNAL_86_GC_PERCENT=60.000
PRIMER_INTERNAL_86_SELF_ANY_TH=0.00
PRIMER_INTERNAL_86_SELF_END_TH=0.00
PRIMER_INTERNAL_86_HAIRPIN_TH=41.60
PRIMER_INTERNAL_87_PENALTY=6.905174
PRIMER_INTERNAL_87_SEQUENCE=CGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_87=74,25
PRIMER_INTERNAL_87_TM=58.095
PRIMER_INTERNAL_87_GC_PERCENT=52.000
PRIMER_INTERNAL_87_SELF_ANY_TH=0.00
PRIMER_INTERNAL_87_SELF_END_TH=0.00
PRIMER_INTERNAL_87_HAIRPIN_TH=41.28
PRIMER_INTERNAL_88_PENALTY=6.905408
PRIMER_INTERNAL_88_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_88=58,26
PRIMER_INTERNAL_88_TM=59.095
PRIMER_INTERNAL_88_GC_PERCENT=53.846
PRIMER_INTERNAL_88_SELF_ANY_TH=7.03
PRIMER_INTERNAL_88_SELF_END_TH=0.00
PRIMER_INTERNAL_88_HAIRPIN_TH=41.60
PRIMER_INTERNAL_89_PENALTY=6.917157
PRIMER_INTERNAL_89_SEQUENCE=ACACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_89=37,26
PRIMER_INTERNAL_89_TM=59.083
PRIMER_INTERNAL_89_GC_PERCENT=46.154
PRIMER_INTERNAL_89_SELF_ANY_TH=0.00
PRIMER_INTERNAL_89_SELF_END_TH=0.00
PRIMER_INTERNAL_89_HAIRPIN_TH=0.00
PRIMER_INTERNAL_90_PENALTY=6.956454
PRIMER_INTERNAL_90_SEQUENCE=AGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_90=10,24
PRIMER_INTERNAL_90_TM=57.044
PRIMER_INTERNAL_90_GC_PERCENT=50.000
PRIMER_INTERNAL_90_SELF_ANY_TH=0.00
PRIMER_INTERNAL_90_SELF_END_TH=0.00
PRIMER_INTERNAL_90_HAIRPIN_TH=36.06
PRIMER_INTERNAL_91_PENALTY=6.975446
PRIMER_INTERNAL_91_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_91=73,26
PRIMER_INTERNAL_91_TM=59.025
PRIMER_INTERNAL_91_GC_PERCENT=50.000
PRIMER_INTERNAL_91_SELF_ANY_TH=0.00
PRIMER_INTERNAL_91_SELF_END_TH=0.00
PRIMER_INTERNAL_91_HAIRPIN_TH=38.51
PRIMER_INTERNAL_92_PENALTY=7.015932
PRIMER_INTERNAL_92_SEQUENCE=CCCGACCCAAATCGATAGCTACGATGA
PRIMER_INTERNAL_92=78,27
PRIMER_INTERNAL_92_TM=60.016
PRIMER_INTERNAL_92_GC_PERCENT=51.852
PRIMER_INTERNAL_92_SELF_ANY_TH=18.40
PRIMER_INTERNAL_92_SELF_END_TH=5.09
PRIMER_INTERNAL_92_HAIRPIN_TH=43.02
PRIMER_INTERNAL_93_PENALTY=7.015932
PRIMER_INTERNAL_93_SEQUENCE=TCCCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_93=77,27
PRIMER_INTERNAL_93_TM=60.016
PRIMER_INTERNAL_93_GC_PERCENT=51.852
PRIMER_INTERNAL_93_SELF_ANY_TH=18.40
PRIMER_INTERNAL_93_SELF_END_TH=11.69
PRIMER_INTERNAL_93_HAIRPIN_TH=43.02
PRIMER_INTERNAL_94_PENALTY=7.051167
PRIMER_INTERNAL_94_SEQUENCE=TTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_94=61,25
PRIMER_INTERNAL_94_TM=62.051
PRIMER_INTERNAL_94_GC_PERCENT=60.000
PRIMER_INTERNAL_94_SELF_ANY_TH=7.03
PRIMER_INTERNAL_94_SELF_END_TH=0.00
PRIMER_INTERNAL_94_HAIRPIN_TH=41.60
PRIMER_INTERNAL_95_PENALTY=7.056335
PRIMER_INTERNAL_95_SEQUENCE=GATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_95=75,26
PRIMER_INTERNAL_95_TM=58.944
PRIMER_INTERNAL_95_GC_PERCENT=53.846
PRIMER_INTERNAL_95_SELF_ANY_TH=0.00
PRIMER_INTERNAL_95_SELF_END_TH=0.00
PRIMER_INTERNAL_95_HAIRPIN_TH=41.28
PRIMER_INTERNAL_96_PENALTY=7.056335
PRIMER_INTERNAL_96_SEQUENCE=CGATCCCGACCCAAATCGATAGCTAC
PRIMER_INTERNAL_96=74,26
PRIMER_INTERNAL_96_TM=58.944
PRIMER_INTERNAL_96_GC_PERCENT=53.846
PRIMER_INTERNAL_96_SELF_ANY_TH=0.00
PRIMER_INTERNAL_96_SELF_END_TH=0.00
PRIMER_INTERNAL_96_HAIRPIN_TH=41.28
PRIMER_INTERNAL_97_PENALTY=7.066919
PRIMER_INTERNAL_97_SEQUENCE=CACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_97=38,25
PRIMER_INTERNAL_97_TM=57.933
PRIMER_INTERNAL_97_GC_PERCENT=48.000
PRIMER_INTERNAL_97_SELF_ANY_TH=0.00
PRIMER_INTERNAL_97_SELF_END_TH=0.00
PRIMER_INTERNAL_97_HAIRPIN_TH=0.00
PRIMER_INTERNAL_98_PENALTY=7.070364
PRIMER_INTERNAL_98_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_98=56,25
PRIMER_INTERNAL_98_TM=57.930
PRIMER_INTERNAL_98_GC_PERCENT=52.000
PRIMER_INTERNAL_98_SELF_ANY_TH=7.03
PRIMER_INTERNAL_98_SELF_END_TH=0.00
PRIMER_INTERNAL_98_HAIRPIN_TH=38.81
PRIMER_INTERNAL_99_PENALTY=7.094172
PRIMER_INTERNAL_99_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_99=54,27
PRIMER_INTERNAL_99_TM=59.906
PRIMER_INTERNAL_99_GC_PERCENT=51.852
PRIMER_INTERNAL_99_SELF_ANY_TH=7.03
PRIMER_INTERNAL_99_SELF_END_TH=0.00
PRIMER_INTERNAL_99_HAIRPIN_TH=38.81
PRIMER_INTERNAL_100_PENALTY=7.124042
PRIMER_INTERNAL_100_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_100=71,26
PRIMER_INTERNAL_100_TM=58.876
PRIMER_INTERNAL_100_GC_PERCENT=50.000
PRIMER_INTERNAL_100_SELF_ANY_TH=0.00
PRIMER_INTERNAL_100_SELF_END_TH=0.00
PRIMER_INTERNAL_100_HAIRPIN_TH=41.68
PRIMER_INTERNAL_101_PENALTY=7.139284
PRIMER_INTERNAL_101_SEQUENCE=CACACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_101=36,27
PRIMER_INTERNAL_101_TM=59.861
PRIMER_INTERNAL_101_GC_PERCENT=48.148
PRIMER_INTERNAL_101_SELF_ANY_TH=0.00
PRIMER_INTERNAL_101_SELF_END_TH=0.00
PRIMER_INTERNAL_101_HAIRPIN_TH=0.00
PRIMER_INTERNAL_102_PENALTY=7.145199
PRIMER_INTERNAL_102_SEQUENCE=GCCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_102=68,26
PRIMER_INTERNAL_102_TM=61.145
PRIMER_INTERNAL_102_GC_PERCENT=53.846
PRIMER_INTERNAL_102_SELF_ANY_TH=2.97
PRIMER_INTERNAL_102_SELF_END_TH=0.00
PRIMER_INTERNAL_102_HAIRPIN_TH=41.68
PRIMER_INTERNAL_103_PENALTY=7.192525
PRIMER_INTERNAL_103_SEQUENCE=GATCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_103=75,27
PRIMER_INTERNAL_103_TM=59.807
PRIMER_INTERNAL_103_GC_PERCENT=51.852
PRIMER_INTERNAL_103_SELF_ANY_TH=12.68
PRIMER_INTERNAL_103_SELF_END_TH=12.68
PRIMER_INTERNAL_103_HAIRPIN_TH=41.28
PRIMER_INTERNAL_104_PENALTY=7.192525
PRIMER_INTERNAL_104_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTAC
PRIMER_INTERNAL_104=73,27
PRIMER_INTERNAL_104_TM=59.807
PRIMER_INTERNAL_104_GC_PERCENT=51.852
PRIMER_INTERNAL_104_SELF_ANY_TH=0.00
PRIMER_INTERNAL_104_SELF_END_TH=0.00
PRIMER_INTERNAL_104_HAIRPIN_TH=38.51
PRIMER_INTERNAL_105_PENALTY=7.193259
PRIMER_INTERNAL_105_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_105=55,26
PRIMER_INTERNAL_105_TM=58.807
PRIMER_INTERNAL_105_GC_PERCENT=53.846
PRIMER_INTERNAL_105_SELF_ANY_TH=7.03
PRIMER_INTERNAL_105_SELF_END_TH=0.00
PRIMER_INTERNAL_105_HAIRPIN_TH=38.81
PRIMER_INTERNAL_106_PENALTY=7.239217
PRIMER_INTERNAL_106_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGA
PRIMER_INTERNAL_106=52,25
PRIMER_INTERNAL_106_TM=57.761
PRIMER_INTERNAL_106_GC_PERCENT=48.000
PRIMER_INTERNAL_106_SELF_ANY_TH=7.03
PRIMER_INTERNAL_106_SELF_END_TH=0.00
PRIMER_INTERNAL_106_HAIRPIN_TH=36.70
PRIMER_INTERNAL_107_PENALTY=7.243997
PRIMER_INTERNAL_107_SEQUENCE=TGGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_107=65,25
PRIMER_INTERNAL_107_TM=62.244
PRIMER_INTERNAL_107_GC_PERCENT=56.000
PRIMER_INTERNAL_107_SELF_ANY_TH=0.00
PRIMER_INTERNAL_107_SELF_END_TH=0.00
PRIMER_INTERNAL_107_HAIRPIN_TH=41.29
PRIMER_INTERNAL_108_PENALTY=7.246964
PRIMER_INTERNAL_108_SEQUENCE=ACACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_108=43,26
PRIMER_INTERNAL_108_TM=58.753
PRIMER_INTERNAL_108_GC_PERCENT=50.000
PRIMER_INTERNAL_108_SELF_ANY_TH=0.00
PRIMER_INTERNAL_108_SELF_END_TH=0.00
PRIMER_INTERNAL_108_HAIRPIN_TH=38.74
PRIMER_INTERNAL_109_PENALTY=7.250024
PRIMER_INTERNAL_109_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_109=71,27
PRIMER_INTERNAL_109_TM=59.750
PRIMER_INTERNAL_109_GC_PERCENT=48.148
PRIMER_INTERNAL_109_SELF_ANY_TH=0.00
PRIMER_INTERNAL_109_SELF_END_TH=0.00
PRIMER_INTERNAL_109_HAIRPIN_TH=41.68
PRIMER_INTERNAL_110_PENALTY=7.293083
PRIMER_INTERNAL_110_SEQUENCE=CACACAGGTATTAGTGGGCCATTCG
PRIMER_INTERNAL_110=51,25
PRIMER_INTERNAL_110_TM=57.707
PRIMER_INTERNAL_110_GC_PERCENT=52.000
PRIMER_INTERNAL_110_SELF_ANY_TH=7.03
PRIMER_INTERNAL_110_SELF_END_TH=0.00
PRIMER_INTERNAL_110_HAIRPIN_TH=36.70
PRIMER_INTERNAL_111_PENALTY=7.342607
PRIMER_INTERNAL_111_SEQUENCE=CATTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_111=70,27
PRIMER_INTERNAL_111_TM=59.657
PRIMER_INTERNAL_111_GC_PERCENT=51.852
PRIMER_INTERNAL_111_SELF_ANY_TH=0.00
PRIMER_INTERNAL_111_SELF_END_TH=0.00
PRIMER_INTERNAL_111_HAIRPIN_TH=41.68
PRIMER_INTERNAL_112_PENALTY=7.354919
PRIMER_INTERNAL_112_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGA
PRIMER_INTERNAL_112=51,26
PRIMER_INTERNAL_112_TM=58.645
PRIMER_INTERNAL_112_GC_PERCENT=50.000
PRIMER_INTERNAL_112_SELF_ANY_TH=7.03
PRIMER_INTERNAL_112_SELF_END_TH=0.00
PRIMER_INTERNAL_112_HAIRPIN_TH=36.70
PRIMER_INTERNAL_113_PENALTY=7.401440
PRIMER_INTERNAL_113_SEQUENCE=CCATTCGATCCCGACCCAAATCGATA
PRIMER_INTERNAL_113=69,26
PRIMER_INTERNAL_113_TM=58.599
PRIMER_INTERNAL_113_GC_PERCENT=50.000
PRIMER_INTERNAL_113_SELF_ANY_TH=0.00
PRIMER_INTERNAL_113_SELF_END_TH=0.00
PRIMER_INTERNAL_113_HAIRPIN_TH=41.68
PRIMER_INTERNAL_114_PENALTY=7.408271
PRIMER_INTERNAL_114_SEQUENCE=CACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_114=44,25
PRIMER_INTERNAL_114_TM=57.592
PRIMER_INTERNAL_114_GC_PERCENT=52.000
PRIMER_INTERNAL_114_SELF_ANY_TH=0.00
PRIMER_INTERNAL_114_SELF_END_TH=0.00
PRIMER_INTERNAL_114_HAIRPIN_TH=38.74
PRIMER_INTERNAL_115_PENALTY=7.451841
PRIMER_INTERNAL_115_SEQUENCE=CACACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_115=42,27
PRIMER_INTERNAL_115_TM=59.548
PRIMER_INTERNAL_115_GC_PERCENT=51.852
PRIMER_INTERNAL_115_SELF_ANY_TH=0.00
PRIMER_INTERNAL_115_SELF_END_TH=0.00
PRIMER_INTERNAL_115_HAIRPIN_TH=38.74
PRIMER_INTERNAL_116_PENALTY=7.534114
PRIMER_INTERNAL_116_SEQUENCE=CCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_116=79,25
PRIMER_INTERNAL_116_TM=57.466
PRIMER_INTERNAL_116_GC_PERCENT=52.000
PRIMER_INTERNAL_116_SELF_ANY_TH=18.40
PRIMER_INTERNAL_116_SELF_END_TH=11.69
PRIMER_INTERNAL_116_HAIRPIN_TH=43.02
PRIMER_INTERNAL_117_PENALTY=7.537719
PRIMER_INTERNAL_117_SEQUENCE=CGACCCAAATCGATAGCTACGATGACG
PRIMER_INTERNAL_117=80,27
PRIMER_INTERNAL_117_TM=59.462
PRIMER_INTERNAL_117_GC_PERCENT=51.852
PRIMER_INTERNAL_117_SELF_ANY_TH=18.40
PRIMER_INTERNAL_117_SELF_END_TH=13.91
PRIMER_INTERNAL_117_HAIRPIN_TH=43.02
PRIMER_INTERNAL_118_PENALTY=7.602591
PRIMER_INTERNAL_118_SEQUENCE=CCGACCCAAATCGATAGCTACGATGA
PRIMER_INTERNAL_118=79,26
PRIMER_INTERNAL_118_TM=58.397
PRIMER_INTERNAL_118_GC_PERCENT=50.000
PRIMER_INTERNAL_118_SELF_ANY_TH=18.40
PRIMER_INTERNAL_118_SELF_END_TH=5.09
PRIMER_INTERNAL_118_HAIRPIN_TH=43.02
PRIMER_INTERNAL_119_PENALTY=7.672898
PRIMER_INTERNAL_119_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_119=76,27
PRIMER_INTERNAL_119_TM=59.327
PRIMER_INTERNAL_119_GC_PERCENT=48.148
PRIMER_INTERNAL_119_SELF_ANY_TH=14.48
PRIMER_INTERNAL_119_SELF_END_TH=14.48
PRIMER_INTERNAL_119_HAIRPIN_TH=46.54
PRIMER_INTERNAL_120_PENALTY=7.693260
PRIMER_INTERNAL_120_SEQUENCE=GCCATTCGATCCCGACCCAAATCGATA
PRIMER_INTERNAL_120=68,27
PRIMER_INTERNAL_120_TM=60.693
PRIMER_INTERNAL_120_GC_PERCENT=51.852
PRIMER_INTERNAL_120_SELF_ANY_TH=0.00
PRIMER_INTERNAL_120_SELF_END_TH=0.00
PRIMER_INTERNAL_120_HAIRPIN_TH=41.68
PRIMER_INTERNAL_121_PENALTY=7.693499
PRIMER_INTERNAL_121_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_121=58,27
PRIMER_INTERNAL_121_TM=60.693
PRIMER_INTERNAL_121_GC_PERCENT=55.556
PRIMER_INTERNAL_121_SELF_ANY_TH=7.03
PRIMER_INTERNAL_121_SELF_END_TH=0.00
PRIMER_INTERNAL_121_HAIRPIN_TH=41.60
PRIMER_INTERNAL_122_PENALTY=7.693499
PRIMER_INTERNAL_122_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_122=57,27
PRIMER_INTERNAL_122_TM=60.693
PRIMER_INTERNAL_122_GC_PERCENT=55.556
PRIMER_INTERNAL_122_SELF_ANY_TH=7.03
PRIMER_INTERNAL_122_SELF_END_TH=0.00
PRIMER_INTERNAL_122_HAIRPIN_TH=41.60
PRIMER_INTERNAL_123_PENALTY=7.694218
PRIMER_INTERNAL_123_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_123=55,27
PRIMER_INTERNAL_123_TM=60.694
PRIMER_INTERNAL_123_GC_PERCENT=55.556
PRIMER_INTERNAL_123_SELF_ANY_TH=7.03
PRIMER_INTERNAL_123_SELF_END_TH=0.00
PRIMER_INTERNAL_123_HAIRPIN_TH=39.49
PRIMER_INTERNAL_124_PENALTY=7.696389
PRIMER_INTERNAL_124_SEQUENCE=ACACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_124=35,27
PRIMER_INTERNAL_124_TM=60.696
PRIMER_INTERNAL_124_GC_PERCENT=48.148
PRIMER_INTERNAL_124_SELF_ANY_TH=0.00
PRIMER_INTERNAL_124_SELF_END_TH=0.00
PRIMER_INTERNAL_124_HAIRPIN_TH=0.00
PRIMER_INTERNAL_125_PENALTY=7.723400
PRIMER_INTERNAL_125_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_125=72,27
PRIMER_INTERNAL_125_TM=59.277
PRIMER_INTERNAL_125_GC_PERCENT=48.148
PRIMER_INTERNAL_125_SELF_ANY_TH=0.00
PRIMER_INTERNAL_125_SELF_END_TH=0.00
PRIMER_INTERNAL_125_HAIRPIN_TH=41.68
PRIMER_INTERNAL_126_PENALTY=7.753661
PRIMER_INTERNAL_126_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_126=54,26
PRIMER_INTERNAL_126_TM=58.246
PRIMER_INTERNAL_126_GC_PERCENT=50.000
PRIMER_INTERNAL_126_SELF_ANY_TH=7.03
PRIMER_INTERNAL_126_SELF_END_TH=5.96
PRIMER_INTERNAL_126_HAIRPIN_TH=0.00
PRIMER_INTERNAL_127_PENALTY=7.785822
PRIMER_INTERNAL_127_SEQUENCE=CGATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_127=74,27
PRIMER_INTERNAL_127_TM=60.786
PRIMER_INTERNAL_127_GC_PERCENT=55.556
PRIMER_INTERNAL_127_SELF_ANY_TH=0.00
PRIMER_INTERNAL_127_SELF_END_TH=0.00
PRIMER_INTERNAL_127_HAIRPIN_TH=41.28
PRIMER_INTERNAL_128_PENALTY=7.802259
PRIMER_INTERNAL_128_SEQUENCE=CCGACCCAAATCGATAGCTACGATGAC
PRIMER_INTERNAL_128=79,27
PRIMER_INTERNAL_128_TM=59.198
PRIMER_INTERNAL_128_GC_PERCENT=51.852
PRIMER_INTERNAL_128_SELF_ANY_TH=18.40
PRIMER_INTERNAL_128_SELF_END_TH=9.31
PRIMER_INTERNAL_128_HAIRPIN_TH=43.02
PRIMER_INTERNAL_129_PENALTY=7.812760
PRIMER_INTERNAL_129_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_129=56,27
PRIMER_INTERNAL_129_TM=60.813
PRIMER_INTERNAL_129_GC_PERCENT=51.852
PRIMER_INTERNAL_129_SELF_ANY_TH=7.03
PRIMER_INTERNAL_129_SELF_END_TH=0.00
PRIMER_INTERNAL_129_HAIRPIN_TH=39.49
PRIMER_INTERNAL_130_PENALTY=7.812835
PRIMER_INTERNAL_130_SEQUENCE=CCATTCGATCCCGACCCAAATCGATAG
PRIMER_INTERNAL_130=69,27
PRIMER_INTERNAL_130_TM=59.187
PRIMER_INTERNAL_130_GC_PERCENT=51.852
PRIMER_INTERNAL_130_SELF_ANY_TH=0.00
PRIMER_INTERNAL_130_SELF_END_TH=0.00
PRIMER_INTERNAL_130_HAIRPIN_TH=41.68
PRIMER_INTERNAL_131_PENALTY=7.928092
PRIMER_INTERNAL_131_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_131=53,27
PRIMER_INTERNAL_131_TM=59.072
PRIMER_INTERNAL_131_GC_PERCENT=51.852
PRIMER_INTERNAL_131_SELF_ANY_TH=7.03
PRIMER_INTERNAL_131_SELF_END_TH=5.96
PRIMER_INTERNAL_131_HAIRPIN_TH=29.02
PRIMER_INTERNAL_132_PENALTY=7.938713
PRIMER_INTERNAL_132_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_132=55,25
PRIMER_INTERNAL_132_TM=57.061
PRIMER_INTERNAL_132_GC_PERCENT=52.000
PRIMER_INTERNAL_132_SELF_ANY_TH=7.03
PRIMER_INTERNAL_132_SELF_END_TH=5.96
PRIMER_INTERNAL_132_HAIRPIN_TH=0.00
PRIMER_INTERNAL_133_PENALTY=7.949148
PRIMER_INTERNAL_133_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_133=64,25
PRIMER_INTERNAL_133_TM=62.949
PRIMER_INTERNAL_133_GC_PERCENT=60.000
PRIMER_INTERNAL_133_SELF_ANY_TH=7.03
PRIMER_INTERNAL_133_SELF_END_TH=0.00
PRIMER_INTERNAL_133_HAIRPIN_TH=43.79
PRIMER_INTERNAL_134_PENALTY=7.979746
PRIMER_INTERNAL_134_SEQUENCE=ACACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_134=41,27
PRIMER_INTERNAL_134_TM=59.020
PRIMER_INTERNAL_134_GC_PERCENT=48.148
PRIMER_INTERNAL_134_SELF_ANY_TH=0.00
PRIMER_INTERNAL_134_SELF_END_TH=0.00
PRIMER_INTERNAL_134_HAIRPIN_TH=36.70
PRIMER_INTERNAL_135_PENALTY=7.992102
PRIMER_INTERNAL_135_SEQUENCE=TAGTGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_135=62,25
PRIMER_INTERNAL_135_TM=62.992
PRIMER_INTERNAL_135_GC_PERCENT=60.000
PRIMER_INTERNAL_135_SELF_ANY_TH=7.03
PRIMER_INTERNAL_135_SELF_END_TH=3.99
PRIMER_INTERNAL_135_HAIRPIN_TH=42.67
PRIMER_INTERNAL_136_PENALTY=7.993148
PRIMER_INTERNAL_136_SEQUENCE=ACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_136=43,25
PRIMER_INTERNAL_136_TM=57.007
PRIMER_INTERNAL_136_GC_PERCENT=48.000
PRIMER_INTERNAL_136_SELF_ANY_TH=0.00
PRIMER_INTERNAL_136_SELF_END_TH=0.00
PRIMER_INTERNAL_136_HAIRPIN_TH=36.70
PRIMER_INTERNAL_137_PENALTY=8.038590
PRIMER_INTERNAL_137_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_137=60,26
PRIMER_INTERNAL_137_TM=62.039
PRIMER_INTERNAL_137_GC_PERCENT=57.692
PRIMER_INTERNAL_137_SELF_ANY_TH=7.03
PRIMER_INTERNAL_137_SELF_END_TH=0.00
PRIMER_INTERNAL_137_HAIRPIN_TH=41.60
PRIMER_INTERNAL_138_PENALTY=8.084641
PRIMER_INTERNAL_138_SEQUENCE=CACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_138=42,26
PRIMER_INTERNAL_138_TM=57.915
PRIMER_INTERNAL_138_GC_PERCENT=50.000
PRIMER_INTERNAL_138_SELF_ANY_TH=0.00
PRIMER_INTERNAL_138_SELF_END_TH=0.00
PRIMER_INTERNAL_138_HAIRPIN_TH=36.70
PRIMER_INTERNAL_139_PENALTY=8.098597
PRIMER_INTERNAL_139_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGAT
PRIMER_INTERNAL_139=52,26
PRIMER_INTERNAL_139_TM=57.901
PRIMER_INTERNAL_139_GC_PERCENT=46.154
PRIMER_INTERNAL_139_SELF_ANY_TH=7.03
PRIMER_INTERNAL_139_SELF_END_TH=2.18
PRIMER_INTERNAL_139_HAIRPIN_TH=36.70
PRIMER_INTERNAL_140_PENALTY=8.106799
PRIMER_INTERNAL_140_SEQUENCE=TCAGTAGACATGACACTGACGATGCA
PRIMER_INTERNAL_140=5,26
PRIMER_INTERNAL_140_TM=57.893
PRIMER_INTERNAL_140_GC_PERCENT=46.154
PRIMER_INTERNAL_140_SELF_ANY_TH=13.87
PRIMER_INTERNAL_140_SELF_END_TH=0.00
PRIMER_INTERNAL_140_HAIRPIN_TH=37.56
PRIMER_INTERNAL_141_PENALTY=8.251191
PRIMER_INTERNAL_141_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGAT
PRIMER_INTERNAL_141=51,27
PRIMER_INTERNAL_141_TM=58.749
PRIMER_INTERNAL_141_GC_PERCENT=48.148
PRIMER_INTERNAL_141_SELF_ANY_TH=7.03
PRIMER_INTERNAL_141_SELF_END_TH=2.18
PRIMER_INTERNAL_141_HAIRPIN_TH=36.70
PRIMER_INTERNAL_142_PENALTY=8.275978
PRIMER_INTERNAL_142_SEQUENCE=GTCAGTAGACATGACACTGACGATGCA
PRIMER_INTERNAL_142=4,27
PRIMER_INTERNAL_142_TM=58.724
PRIMER_INTERNAL_142_GC_PERCENT=48.148
PRIMER_INTERNAL_142_SELF_ANY_TH=22.59
PRIMER_INTERNAL_142_SELF_END_TH=0.00
PRIMER_INTERNAL_142_HAIRPIN_TH=46.00
PRIMER_INTERNAL_143_PENALTY=8.300145
PRIMER_INTERNAL_143_SEQUENCE=ACACACACACACAGCACACAGGTATTA
PRIMER_INTERNAL_143=37,27
PRIMER_INTERNAL_143_TM=58.700
PRIMER_INTERNAL_143_GC_PERCENT=44.444
PRIMER_INTERNAL_143_SELF_ANY_TH=0.00
PRIMER_INTERNAL_143_SELF_END_TH=0.00
PRIMER_INTERNAL_143_HAIRPIN_TH=0.00
PRIMER_INTERNAL_144_PENALTY=8.330872
PRIMER_INTERNAL_144_SEQUENCE=AGTAGACATGACACTGACGATGCAGA
PRIMER_INTERNAL_144=7,26
PRIMER_INTERNAL_144_TM=57.669
PRIMER_INTERNAL_144_GC_PERCENT=46.154
PRIMER_INTERNAL_144_SELF_ANY_TH=0.00
PRIMER_INTERNAL_144_SELF_END_TH=0.00
PRIMER_INTERNAL_144_HAIRPIN_TH=36.06
PRIMER_INTERNAL_145_PENALTY=8.376203
PRIMER_INTERNAL_145_SEQUENCE=GTAGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_145=8,26
PRIMER_INTERNAL_145_TM=57.624
PRIMER_INTERNAL_145_GC_PERCENT=50.000
PRIMER_INTERNAL_145_SELF_ANY_TH=0.00
PRIMER_INTERNAL_145_SELF_END_TH=0.00
PRIMER_INTERNAL_145_HAIRPIN_TH=36.06
PRIMER_INTERNAL_146_PENALTY=8.376203
PRIMER_INTERNAL_146_SEQUENCE=GTCAGTAGACATGACACTGACGATGC
PRIMER_INTERNAL_146=4,26
PRIMER_INTERNAL_146_TM=57.624
PRIMER_INTERNAL_146_GC_PERCENT=50.000
PRIMER_INTERNAL_146_SELF_ANY_TH=22.59
PRIMER_INTERNAL_146_SELF_END_TH=10.24
PRIMER_INTERNAL_146_HAIRPIN_TH=46.00
PRIMER_INTERNAL_147_PENALTY=8.379879
PRIMER_INTERNAL_147_SEQUENCE=CAGTAGACATGACACTGACGATGCAG
PRIMER_INTERNAL_147=6,26
PRIMER_INTERNAL_147_TM=57.620
PRIMER_INTERNAL_147_GC_PERCENT=50.000
PRIMER_INTERNAL_147_SELF_ANY_TH=12.06
PRIMER_INTERNAL_147_SELF_END_TH=0.00
PRIMER_INTERNAL_147_HAIRPIN_TH=37.39
PRIMER_INTERNAL_148_PENALTY=8.418488
PRIMER_INTERNAL_148_SEQUENCE=CACACACACACAGCACACAGGTATTA
PRIMER_INTERNAL_148=38,26
PRIMER_INTERNAL_148_TM=57.582
PRIMER_INTERNAL_148_GC_PERCENT=46.154
PRIMER_INTERNAL_148_SELF_ANY_TH=0.00
PRIMER_INTERNAL_148_SELF_END_TH=0.00
PRIMER_INTERNAL_148_HAIRPIN_TH=0.00
PRIMER_INTERNAL_149_PENALTY=8.423914
PRIMER_INTERNAL_149_SEQUENCE=GACCCAAATCGATAGCTACGATGACG
PRIMER_INTERNAL_149=81,26
PRIMER_INTERNAL_149_TM=57.576
PRIMER_INTERNAL_149_GC_PERCENT=50.000
PRIMER_INTERNAL_149_SELF_ANY_TH=18.40
PRIMER_INTERNAL_149_SELF_END_TH=12.20
PRIMER_INTERNAL_149_HAIRPIN_TH=43.02
PRIMER_INTERNAL_150_PENALTY=8.423914
PRIMER_INTERNAL_150_SEQUENCE=CGACCCAAATCGATAGCTACGATGAC
PRIMER_INTERNAL_150=80,26
PRIMER_INTERNAL_150_TM=57.576
PRIMER_INTERNAL_150_GC_PERCENT=50.000
PRIMER_INTERNAL_150_SELF_ANY_TH=18.40
PRIMER_INTERNAL_150_SELF_END_TH=9.31
PRIMER_INTERNAL_150_HAIRPIN_TH=43.02
PRIMER_INTERNAL_151_PENALTY=8.452836
PRIMER_INTERNAL_151_SEQUENCE=CATTCGATCCCGACCCAAATCGATAG
PRIMER_INTERNAL_151=70,26
PRIMER_INTERNAL_151_TM=57.547
PRIMER_INTERNAL_151_GC_PERCENT=50.000
PRIMER_INTERNAL_151_SELF_ANY_TH=0.00
PRIMER_INTERNAL_151_SELF_END_TH=0.00
PRIMER_INTERNAL_151_HAIRPIN_TH=41.68
PRIMER_INTERNAL_152_PENALTY=8.463323
PRIMER_INTERNAL_152_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGATC
PRIMER_INTERNAL_152=52,27
PRIMER_INTERNAL_152_TM=58.537
PRIMER_INTERNAL_152_GC_PERCENT=48.148
PRIMER_INTERNAL_152_SELF_ANY_TH=7.03
PRIMER_INTERNAL_152_SELF_END_TH=0.00
PRIMER_INTERNAL_152_HAIRPIN_TH=36.70
PRIMER_INTERNAL_153_PENALTY=8.486477
PRIMER_INTERNAL_153_SEQUENCE=CAGTAGACATGACACTGACGATGCAGA
PRIMER_INTERNAL_153=6,27
PRIMER_INTERNAL_153_TM=58.514
PRIMER_INTERNAL_153_GC_PERCENT=48.148
PRIMER_INTERNAL_153_SELF_ANY_TH=12.06
PRIMER_INTERNAL_153_SELF_END_TH=0.00
PRIMER_INTERNAL_153_HAIRPIN_TH=37.39
PRIMER_INTERNAL_154_PENALTY=8.486477
PRIMER_INTERNAL_154_SEQUENCE=TCAGTAGACATGACACTGACGATGCAG
PRIMER_INTERNAL_154=5,27
PRIMER_INTERNAL_154_TM=58.514
PRIMER_INTERNAL_154_GC_PERCENT=48.148
PRIMER_INTERNAL_154_SELF_ANY_TH=13.87
PRIMER_INTERNAL_154_SELF_END_TH=0.00
PRIMER_INTERNAL_154_HAIRPIN_TH=37.56
PRIMER_INTERNAL_155_PENALTY=8.487256
PRIMER_INTERNAL_155_SEQUENCE=AGTAGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_155=7,27
PRIMER_INTERNAL_155_TM=58.513
PRIMER_INTERNAL_155_GC_PERCENT=48.148
PRIMER_INTERNAL_155_SELF_ANY_TH=0.00
PRIMER_INTERNAL_155_SELF_END_TH=0.00
PRIMER_INTERNAL_155_HAIRPIN_TH=36.06
PRIMER_INTERNAL_156_PENALTY=8.514997
PRIMER_INTERNAL_156_SEQUENCE=ACACACACACAGCACACAGGTATTAGT
PRIMER_INTERNAL_156=39,27
PRIMER_INTERNAL_156_TM=58.485
PRIMER_INTERNAL_156_GC_PERCENT=44.444
PRIMER_INTERNAL_156_SELF_ANY_TH=0.00
PRIMER_INTERNAL_156_SELF_END_TH=0.00
PRIMER_INTERNAL_156_HAIRPIN_TH=0.00
PRIMER_INTERNAL_157_PENALTY=8.547914
PRIMER_INTERNAL_157_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_157=59,27
PRIMER_INTERNAL_157_TM=61.548
PRIMER_INTERNAL_157_GC_PERCENT=55.556
PRIMER_INTERNAL_157_SELF_ANY_TH=7.03
PRIMER_INTERNAL_157_SELF_END_TH=0.00
PRIMER_INTERNAL_157_HAIRPIN_TH=41.60
PRIMER_INTERNAL_158_PENALTY=8.589885
PRIMER_INTERNAL_158_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATC
PRIMER_INTERNAL_158=53,26
PRIMER_INTERNAL_158_TM=57.410
PRIMER_INTERNAL_158_GC_PERCENT=50.000
PRIMER_INTERNAL_158_SELF_ANY_TH=7.03
PRIMER_INTERNAL_158_SELF_END_TH=0.00
PRIMER_INTERNAL_158_HAIRPIN_TH=29.02
PRIMER_INTERNAL_159_PENALTY=8.642220
PRIMER_INTERNAL_159_SEQUENCE=ACACACACAGCACACAGGTATTAGTG
PRIMER_INTERNAL_159=41,26
PRIMER_INTERNAL_159_TM=57.358
PRIMER_INTERNAL_159_GC_PERCENT=46.154
PRIMER_INTERNAL_159_SELF_ANY_TH=0.00
PRIMER_INTERNAL_159_SELF_END_TH=0.00
PRIMER_INTERNAL_159_HAIRPIN_TH=39.84
PRIMER_INTERNAL_160_PENALTY=8.642220
PRIMER_INTERNAL_160_SEQUENCE=CACACACACAGCACACAGGTATTAGT
PRIMER_INTERNAL_160=40,26
PRIMER_INTERNAL_160_TM=57.358
PRIMER_INTERNAL_160_GC_PERCENT=46.154
PRIMER_INTERNAL_160_SELF_ANY_TH=0.00
PRIMER_INTERNAL_160_SELF_END_TH=0.00
PRIMER_INTERNAL_160_HAIRPIN_TH=0.00
PRIMER_INTERNAL_161_PENALTY=8.642220
PRIMER_INTERNAL_161_SEQUENCE=ACACACACACAGCACACAGGTATTAG
PRIMER_INTERNAL_161=39,26
PRIMER_INTERNAL_161_TM=57.358
PRIMER_INTERNAL_161_GC_PERCENT=46.154
PRIMER_INTERNAL_161_SELF_ANY_TH=0.00
PRIMER_INTERNAL_161_SELF_END_TH=0.00
PRIMER_INTERNAL_161_HAIRPIN_TH=0.00
PRIMER_INTERNAL_162_PENALTY=8.687221
PRIMER_INTERNAL_162_SEQUENCE=TGGGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_162=65,26
PRIMER_INTERNAL_162_TM=62.687
PRIMER_INTERNAL_162_GC_PERCENT=57.692
PRIMER_INTERNAL_162_SELF_ANY_TH=0.00
PRIMER_INTERNAL_162_SELF_END_TH=0.00
PRIMER_INTERNAL_162_HAIRPIN_TH=41.29
PRIMER_INTERNAL_163_PENALTY=8.713667
PRIMER_INTERNAL_163_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_163=67,26
PRIMER_INTERNAL_163_TM=62.714
PRIMER_INTERNAL_163_GC_PERCENT=57.692
PRIMER_INTERNAL_163_SELF_ANY_TH=0.00
PRIMER_INTERNAL_163_SELF_END_TH=0.00
PRIMER_INTERNAL_163_HAIRPIN_TH=42.01
PRIMER_INTERNAL_164_PENALTY=8.787348
PRIMER_INTERNAL_164_SEQUENCE=CACACACACAGCACACAGGTATTAGTG
PRIMER_INTERNAL_164=40,27
PRIMER_INTERNAL_164_TM=58.213
PRIMER_INTERNAL_164_GC_PERCENT=48.148
PRIMER_INTERNAL_164_SELF_ANY_TH=0.00
PRIMER_INTERNAL_164_SELF_END_TH=0.00
PRIMER_INTERNAL_164_HAIRPIN_TH=39.84
PRIMER_INTERNAL_165_PENALTY=8.787348
PRIMER_INTERNAL_165_SEQUENCE=CACACACACACAGCACACAGGTATTAG
PRIMER_INTERNAL_165=38,27
PRIMER_INTERNAL_165_TM=58.213
PRIMER_INTERNAL_165_GC_PERCENT=48.148
PRIMER_INTERNAL_165_SELF_ANY_TH=0.00
PRIMER_INTERNAL_165_SELF_END_TH=0.00
PRIMER_INTERNAL_165_HAIRPIN_TH=0.00
PRIMER_INTERNAL_166_PENALTY=8.904972
PRIMER_INTERNAL_166_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_166=64,26
PRIMER_INTERNAL_166_TM=62.905
PRIMER_INTERNAL_166_GC_PERCENT=57.692
PRIMER_INTERNAL_166_SELF_ANY_TH=7.03
PRIMER_INTERNAL_166_SELF_END_TH=0.00
PRIMER_INTERNAL_166_HAIRPIN_TH=43.79
PRIMER_INTERNAL_167_PENALTY=9.679371
PRIMER_INTERNAL_167_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_167=67,27
PRIMER_INTERNAL_167_TM=62.679
PRIMER_INTERNAL_167_GC_PERCENT=55.556
PRIMER_INTERNAL_167_SELF_ANY_TH=0.00
PRIMER_INTERNAL_167_SELF_END_TH=0.00
PRIMER_INTERNAL_167_HAIRPIN_TH=41.68
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
P3_FILE_FLAG=1
SEQUENCE_ID=pick_primer_list_and_file_flag_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=33
PRIMER_RIGHT_NUM_RETURNED=33
PRIMER_INTERNAL_NUM_RETURNED=73
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_LEFT_0=118,20
PRIMER_RIGHT_0=137,20
PRIMER_INTERNAL_0=125,20
PRIMER_LEFT_0_TM=58.514
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=59.906
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_1_PENALTY=1.160593
PRIMER_RIGHT_1_PENALTY=1.160593
PRIMER_INTERNAL_1_PENALTY=0.301069
PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_1=122,19
PRIMER_RIGHT_1=140,19
PRIMER_INTERNAL_1=126,20
PRIMER_LEFT_1_TM=59.161
PRIMER_RIGHT_1_TM=59.161
PRIMER_INTERNAL_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_SELF_ANY_TH=7.82
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=37.44
PRIMER_INTERNAL_1_HAIRPIN_TH=36.42
PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_1_END_STABILITY=5.0300
PRIMER_RIGHT_1_END_STABILITY=2.4400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_2_PENALTY=1.656085
PRIMER_RIGHT_2_PENALTY=1.656085
PRIMER_INTERNAL_2_PENALTY=0.900305
PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA
PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG
PRIMER_LEFT_2=117,21
PRIMER_RIGHT_2=137,21
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=58.344
PRIMER_INTERNAL_2_TM=59.100
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.14
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=5.3400
PRIMER_RIGHT_2_END_STABILITY=1.7500
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=8.6247
PRIMER_LEFT_3_PENALTY=1.695359
PRIMER_RIGHT_3_PENALTY=1.695359
PRIMER_INTERNAL_3_PENALTY=0.956520
PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC
PRIMER_LEFT_3=53,21
PRIMER_RIGHT_3=73,21
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=58.305
PRIMER_RIGHT_3_TM=58.305
PRIMER_INTERNAL_3_TM=59.043
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=36.42
PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.7700
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_4_PENALTY=1.831685
PRIMER_RIGHT_4_PENALTY=1.831685
PRIMER_INTERNAL_4_PENALTY=1.018091
PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA
PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT
PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=84,20
PRIMER_INTERNAL_4=125,21
PRIMER_LEFT_4_TM=57.168
PRIMER_RIGHT_4_TM=57.168
PRIMER_INTERNAL_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=57.143
PRIMER_INTERNAL_4_SELF_ANY_TH=7.82
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=35.45
PRIMER_RIGHT_4_HAIRPIN_TH=31.80
PRIMER_INTERNAL_4_HAIRPIN_TH=36.42
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=2.1500
PRIMER_RIGHT_4_END_STABILITY=4.8400
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.8143
PRIMER_LEFT_5_PENALTY=1.902705
PRIMER_RIGHT_5_PENALTY=1.902705
PRIMER_INTERNAL_5_PENALTY=1.178696
PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA
PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_5=62,20
PRIMER_RIGHT_5=81,20
PRIMER_INTERNAL_5=124,21
PRIMER_LEFT_5_TM=57.097
PRIMER_RIGHT_5_TM=57.097
PRIMER_INTERNAL_5_TM=60.179
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=57.143
PRIMER_INTERNAL_5_SELF_ANY_TH=7.82
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=36.42
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=2.7400
PRIMER_RIGHT_5_END_STABILITY=2.5200
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=29.7752
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_6_PENALTY=1.902705
PRIMER_RIGHT_6_PENALTY=1.902705
PRIMER_INTERNAL_6_PENALTY=1.372876
PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT
PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA
PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_6=61,20
PRIMER_RIGHT_6=80,20
PRIMER_INTERNAL_6=126,21
PRIMER_LEFT_6_TM=57.097
PRIMER_RIGHT_6_TM=57.097
PRIMER_INTERNAL_6_TM=60.373
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=7.82
PRIMER_LEFT_6_SELF_ANY_TH=0.00
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=36.42
PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=3.5500
PRIMER_RIGHT_6_END_STABILITY=2.1000
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.0753
PRIMER_LEFT_7_PENALTY=1.950877
PRIMER_RIGHT_7_PENALTY=1.950877
PRIMER_INTERNAL_7_PENALTY=1.393730
PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG
PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA
PRIMER_LEFT_7=119,19
PRIMER_RIGHT_7=137,19
PRIMER_INTERNAL_7=126,19
PRIMER_LEFT_7_TM=58.049
PRIMER_RIGHT_7_TM=58.049
PRIMER_INTERNAL_7_TM=59.606
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_INTERNAL_7_GC_PERCENT=63.158
PRIMER_INTERNAL_7_SELF_ANY_TH=7.82
PRIMER_LEFT_7_SELF_ANY_TH=0.00
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=0.00
PRIMER_RIGHT_7_HAIRPIN_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=36.42
PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=5.3400
PRIMER_RIGHT_7_END_STABILITY=3.5300
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_8_PENALTY=1.972625
PRIMER_RIGHT_8_PENALTY=1.972625
PRIMER_INTERNAL_8_PENALTY=1.595076
PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA
PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_8=56,21
PRIMER_RIGHT_8=76,21
PRIMER_INTERNAL_8=123,21
PRIMER_LEFT_8_TM=58.027
PRIMER_RIGHT_8_TM=58.027
PRIMER_INTERNAL_8_TM=59.405
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_INTERNAL_8_GC_PERCENT=57.143
PRIMER_INTERNAL_8_SELF_ANY_TH=0.00
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=0.00
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=41.14
PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_LEFT_8_END_STABILITY=3.4100
PRIMER_RIGHT_8_END_STABILITY=3.8600
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=32.2237
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.8066
PRIMER_LEFT_9_PENALTY=1.973945
PRIMER_RIGHT_9_PENALTY=1.973945
PRIMER_INTERNAL_9_PENALTY=2.007622
PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC
PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT
PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_9=55,21
PRIMER_RIGHT_9=75,21
PRIMER_INTERNAL_9=121,22
PRIMER_LEFT_9_TM=58.026
PRIMER_RIGHT_9_TM=58.026
PRIMER_INTERNAL_9_TM=59.992
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_INTERNAL_9_GC_PERCENT=59.091
PRIMER_INTERNAL_9_SELF_ANY_TH=0.00
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=0.00
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=0.00
PRIMER_RIGHT_9_HAIRPIN_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=0.00
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=3.0100
PRIMER_RIGHT_9_END_STABILITY=4.0000
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=27.4392
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=5.5040
PRIMER_LEFT_10_PENALTY=1.973945
PRIMER_RIGHT_10_PENALTY=1.973945
PRIMER_INTERNAL_10_PENALTY=2.235780
PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT
PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC
PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG
PRIMER_LEFT_10=54,21
PRIMER_RIGHT_10=74,21
PRIMER_INTERNAL_10=125,19
PRIMER_LEFT_10_TM=58.026
PRIMER_RIGHT_10_TM=58.026
PRIMER_INTERNAL_10_TM=58.764
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_INTERNAL_10_GC_PERCENT=63.158
PRIMER_INTERNAL_10_SELF_ANY_TH=0.00
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=0.00
PRIMER_RIGHT_10_HAIRPIN_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.14
PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=3.1600
PRIMER_RIGHT_10_END_STABILITY=3.8500
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=19.5725
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=9.9844
PRIMER_LEFT_11_PENALTY=2.120321
PRIMER_RIGHT_11_PENALTY=2.120321
PRIMER_INTERNAL_11_PENALTY=2.240074
PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA
PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT
PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_11=64,21
PRIMER_RIGHT_11=84,21
PRIMER_INTERNAL_11=124,22
PRIMER_LEFT_11_TM=57.880
PRIMER_RIGHT_11_TM=57.880
PRIMER_INTERNAL_11_TM=60.240
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_INTERNAL_11_GC_PERCENT=54.545
PRIMER_INTERNAL_11_SELF_ANY_TH=7.82
PRIMER_LEFT_11_SELF_ANY_TH=0.00
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=35.45
PRIMER_RIGHT_11_HAIRPIN_TH=31.80
PRIMER_INTERNAL_11_HAIRPIN_TH=36.42
PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=2.1500
PRIMER_RIGHT_11_END_STABILITY=3.6000
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8905
PRIMER_LEFT_12_PENALTY=2.142778
PRIMER_RIGHT_12_PENALTY=2.142778
PRIMER_INTERNAL_12_PENALTY=2.299276
PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_12=119,20
PRIMER_RIGHT_12=138,20
PRIMER_INTERNAL_12=122,22
PRIMER_LEFT_12_TM=61.143
PRIMER_RIGHT_12_TM=61.143
PRIMER_INTERNAL_12_TM=60.299
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=59.091
PRIMER_INTERNAL_12_SELF_ANY_TH=0.00
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=0.00
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.14
PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=5.3400
PRIMER_RIGHT_12_END_STABILITY=3.5300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_13_PENALTY=2.299089
PRIMER_RIGHT_13_PENALTY=2.299089
PRIMER_INTERNAL_13_PENALTY=2.427391
PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC
PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_13=123,19
PRIMER_RIGHT_13=141,19
PRIMER_INTERNAL_13=123,22
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.299
PRIMER_INTERNAL_13_TM=60.427
PRIMER_LEFT_13_GC_PERCENT=52.632
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_INTERNAL_13_GC_PERCENT=54.545
PRIMER_INTERNAL_13_SELF_ANY_TH=7.82
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=0.00
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=0.00
PRIMER_RIGHT_13_HAIRPIN_TH=37.44
PRIMER_INTERNAL_13_HAIRPIN_TH=36.42
PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_13_END_STABILITY=4.7000
PRIMER_RIGHT_13_END_STABILITY=2.4300
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_14_PENALTY=2.402628
PRIMER_RIGHT_14_PENALTY=2.402628
PRIMER_INTERNAL_14_PENALTY=2.610811
PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT
PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_14=62,21
PRIMER_RIGHT_14=82,21
PRIMER_INTERNAL_14=125,22
PRIMER_LEFT_14_TM=57.597
PRIMER_RIGHT_14_TM=57.597
PRIMER_INTERNAL_14_TM=60.611
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_INTERNAL_14_GC_PERCENT=59.091
PRIMER_INTERNAL_14_SELF_ANY_TH=7.82
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=0.00
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=0.00
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=36.42
PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=2.2900
PRIMER_RIGHT_14_END_STABILITY=2.5200
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=30.4432
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_15_PENALTY=2.402628
PRIMER_RIGHT_15_PENALTY=2.402628
PRIMER_INTERNAL_15_PENALTY=2.740095
PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT
PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT
PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT
PRIMER_LEFT_15=60,21
PRIMER_RIGHT_15=80,21
PRIMER_INTERNAL_15=127,19
PRIMER_LEFT_15_TM=57.597
PRIMER_RIGHT_15_TM=57.597
PRIMER_INTERNAL_15_TM=58.260
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_INTERNAL_15_GC_PERCENT=63.158
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=0.00
PRIMER_RIGHT_15_HAIRPIN_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=36.42
PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=3.5500
PRIMER_RIGHT_15_END_STABILITY=1.9800
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0593
PRIMER_LEFT_16_PENALTY=2.455619
PRIMER_RIGHT_16_PENALTY=2.455619
PRIMER_INTERNAL_16_PENALTY=2.880381
PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG
PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC
PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_16=63,21
PRIMER_RIGHT_16=83,21
PRIMER_INTERNAL_16=120,22
PRIMER_LEFT_16_TM=57.544
PRIMER_RIGHT_16_TM=57.544
PRIMER_INTERNAL_16_TM=59.120
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_INTERNAL_16_GC_PERCENT=54.545
PRIMER_INTERNAL_16_SELF_ANY_TH=0.00
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=0.00
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=0.00
PRIMER_RIGHT_16_HAIRPIN_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=0.00
PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_LEFT_16_END_STABILITY=2.3900
PRIMER_RIGHT_16_END_STABILITY=2.7800
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=25.0463
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8601
PRIMER_LEFT_17_PENALTY=2.539776
PRIMER_RIGHT_17_PENALTY=2.539776
PRIMER_INTERNAL_17_PENALTY=2.939429
PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC
PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_17=122,20
PRIMER_RIGHT_17=141,20
PRIMER_INTERNAL_17=122,21
PRIMER_LEFT_17_TM=61.540
PRIMER_RIGHT_17_TM=61.540
PRIMER_INTERNAL_17_TM=58.061
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_TH=37.44
PRIMER_INTERNAL_17_HAIRPIN_TH=0.00
PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_17_END_STABILITY=4.7000
PRIMER_RIGHT_17_END_STABILITY=2.4400
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_18_PENALTY=2.539776
PRIMER_RIGHT_18_PENALTY=2.539776
PRIMER_INTERNAL_18_PENALTY=2.939429
PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_18=121,20
PRIMER_RIGHT_18=140,20
PRIMER_INTERNAL_18=121,21
PRIMER_LEFT_18_TM=61.540
PRIMER_RIGHT_18_TM=61.540
PRIMER_INTERNAL_18_TM=58.061
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_INTERNAL_18_GC_PERCENT=57.143
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_TH=37.44
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_18_END_STABILITY=5.0300
PRIMER_RIGHT_18_END_STABILITY=3.2800
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_19_PENALTY=2.587613
PRIMER_RIGHT_19_PENALTY=2.587613
PRIMER_INTERNAL_19_PENALTY=3.476553
PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC
PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_19=120,19
PRIMER_RIGHT_19=138,19
PRIMER_INTERNAL_19=123,23
PRIMER_LEFT_19_TM=60.588
PRIMER_RIGHT_19_TM=60.588
PRIMER_INTERNAL_19_TM=60.477
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_INTERNAL_19_GC_PERCENT=52.174
PRIMER_INTERNAL_19_SELF_ANY_TH=7.82
PRIMER_LEFT_19_SELF_ANY_TH=0.00
PRIMER_RIGHT_19_SELF_ANY_TH=0.00
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=36.42
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_19_END_STABILITY=5.3400
PRIMER_RIGHT_19_END_STABILITY=3.5300
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=2.588667
PRIMER_RIGHT_20_PENALTY=2.588667
PRIMER_INTERNAL_20_PENALTY=3.598972
PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT
PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_20=121,19
PRIMER_RIGHT_20=139,19
PRIMER_INTERNAL_20=119,23
PRIMER_LEFT_20_TM=60.589
PRIMER_RIGHT_20_TM=60.589
PRIMER_INTERNAL_20_TM=59.401
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=52.632
PRIMER_INTERNAL_20_GC_PERCENT=52.174
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=0.00
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=0.00
PRIMER_LEFT_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_TH=37.44
PRIMER_INTERNAL_20_HAIRPIN_TH=0.00
PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_20_END_STABILITY=5.0700
PRIMER_RIGHT_20_END_STABILITY=3.2800
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_21_PENALTY=2.627395
PRIMER_RIGHT_21_PENALTY=2.627395
PRIMER_INTERNAL_21_PENALTY=3.606233
PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA
PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT
PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG
PRIMER_LEFT_21=52,21
PRIMER_RIGHT_21=72,21
PRIMER_INTERNAL_21=126,18
PRIMER_LEFT_21_TM=57.373
PRIMER_RIGHT_21_TM=57.373
PRIMER_INTERNAL_21_TM=58.394
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_INTERNAL_21_GC_PERCENT=66.667
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.14
PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_21_END_STABILITY=4.5200
PRIMER_RIGHT_21_END_STABILITY=3.0600
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=1.1837
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_22_PENALTY=2.678056
PRIMER_RIGHT_22_PENALTY=2.678056
PRIMER_INTERNAL_22_PENALTY=3.829005
PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT
PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA
PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_22=58,21
PRIMER_RIGHT_22=78,21
PRIMER_INTERNAL_22=124,23
PRIMER_LEFT_22_TM=57.322
PRIMER_RIGHT_22_TM=57.322
PRIMER_INTERNAL_22_TM=60.829
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_INTERNAL_22_GC_PERCENT=56.522
PRIMER_INTERNAL_22_SELF_ANY_TH=7.82
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=0.00
PRIMER_RIGHT_22_HAIRPIN_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=36.42
PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_22_END_STABILITY=3.4100
PRIMER_RIGHT_22_END_STABILITY=2.1500
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=25.2513
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=19.0855
PRIMER_LEFT_23_PENALTY=2.780624
PRIMER_RIGHT_23_PENALTY=2.780624
PRIMER_INTERNAL_23_PENALTY=3.865064
PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT
PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_23=62,19
PRIMER_RIGHT_23=80,19
PRIMER_INTERNAL_23=120,21
PRIMER_LEFT_23_TM=57.219
PRIMER_RIGHT_23_TM=57.219
PRIMER_INTERNAL_23_TM=57.135
PRIMER_LEFT_23_GC_PERCENT=47.368
PRIMER_RIGHT_23_GC_PERCENT=47.368
PRIMER_INTERNAL_23_GC_PERCENT=52.381
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=0.00
PRIMER_RIGHT_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_23_END_STABILITY=3.5500
PRIMER_RIGHT_23_END_STABILITY=2.5200
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_24_PENALTY=2.999071
PRIMER_RIGHT_24_PENALTY=2.999071
PRIMER_INTERNAL_24_PENALTY=3.905453
PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA
PRIMER_LEFT_24=120,20
PRIMER_RIGHT_24=139,20
PRIMER_INTERNAL_24=127,18
PRIMER_LEFT_24_TM=61.999
PRIMER_RIGHT_24_TM=61.999
PRIMER_INTERNAL_24_TM=58.095
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_INTERNAL_24_GC_PERCENT=66.667
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=0.00
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=0.00
PRIMER_RIGHT_24_HAIRPIN_TH=37.44
PRIMER_INTERNAL_24_HAIRPIN_TH=36.42
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_24_END_STABILITY=5.0700
PRIMER_RIGHT_24_END_STABILITY=3.5300
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_25_PENALTY=2.999229
PRIMER_RIGHT_25_PENALTY=2.999229
PRIMER_INTERNAL_25_PENALTY=3.954555
PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG
PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC
PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_25=59,21
PRIMER_RIGHT_25=79,21
PRIMER_INTERNAL_25=120,23
PRIMER_LEFT_25_TM=57.001
PRIMER_RIGHT_25_TM=57.001
PRIMER_INTERNAL_25_TM=60.955
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_INTERNAL_25_GC_PERCENT=56.522
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=0.00
PRIMER_RIGHT_25_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=0.00
PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_25_END_STABILITY=3.6600
PRIMER_RIGHT_25_END_STABILITY=2.0800
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=30.7683
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=20.4326
PRIMER_LEFT_26_PENALTY=2.999229
PRIMER_RIGHT_26_PENALTY=2.999229
PRIMER_INTERNAL_26_PENALTY=4.240075
PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC
PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG
PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_26=57,21
PRIMER_RIGHT_26=77,21
PRIMER_INTERNAL_26=122,23
PRIMER_LEFT_26_TM=57.001
PRIMER_RIGHT_26_TM=57.001
PRIMER_INTERNAL_26_TM=61.240
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=56.522
PRIMER_INTERNAL_26_SELF_ANY_TH=7.82
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=0.00
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=0.00
PRIMER_LEFT_26_HAIRPIN_TH=0.00
PRIMER_RIGHT_26_HAIRPIN_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=36.42
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=3.5100
PRIMER_RIGHT_26_END_STABILITY=2.9000
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=20.2809
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.6247
PRIMER_LEFT_27_PENALTY=3.455227
PRIMER_RIGHT_27_PENALTY=3.455227
PRIMER_INTERNAL_27_PENALTY=4.486709
PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_27=118,21
PRIMER_RIGHT_27=138,21
PRIMER_INTERNAL_27=119,22
PRIMER_LEFT_27_TM=61.455
PRIMER_RIGHT_27_TM=61.455
PRIMER_INTERNAL_27_TM=57.513
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=50.000
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=0.00
PRIMER_RIGHT_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=0.00
PRIMER_RIGHT_27_SELF_END_TH=0.00
PRIMER_LEFT_27_HAIRPIN_TH=0.00
PRIMER_RIGHT_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=5.3400
PRIMER_RIGHT_27_END_STABILITY=3.0100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_28_PENALTY=3.677573
PRIMER_RIGHT_28_PENALTY=3.677573
PRIMER_INTERNAL_28_PENALTY=4.513970
PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC
PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_28=123,20
PRIMER_RIGHT_28=142,20
PRIMER_INTERNAL_28=118,24
PRIMER_LEFT_28_TM=62.678
PRIMER_RIGHT_28_TM=62.678
PRIMER_INTERNAL_28_TM=59.486
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_INTERNAL_28_GC_PERCENT=50.000
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=37.44
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_28_END_STABILITY=4.3000
PRIMER_RIGHT_28_END_STABILITY=2.4300
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.1346
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_29_PENALTY=4.100862
PRIMER_RIGHT_29_PENALTY=4.100862
PRIMER_INTERNAL_29_PENALTY=5.029817
PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC
PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC
PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_29=128,19
PRIMER_RIGHT_29=146,19
PRIMER_INTERNAL_29=123,24
PRIMER_LEFT_29_TM=62.101
PRIMER_RIGHT_29_TM=62.101
PRIMER_INTERNAL_29_TM=61.030
PRIMER_LEFT_29_GC_PERCENT=63.158
PRIMER_RIGHT_29_GC_PERCENT=63.158
PRIMER_INTERNAL_29_GC_PERCENT=54.167
PRIMER_INTERNAL_29_SELF_ANY_TH=7.82
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=38.29
PRIMER_RIGHT_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=36.42
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_29_END_STABILITY=3.6900
PRIMER_RIGHT_29_END_STABILITY=3.6800
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0362
PRIMER_LEFT_30_PENALTY=4.194272
PRIMER_RIGHT_30_PENALTY=4.194272
PRIMER_INTERNAL_30_PENALTY=5.052238
PRIMER_LEFT_30_SEQUENCE=AAACGCAAAGCACGCTCCC
PRIMER_RIGHT_30_SEQUENCE=GGGAGCGTGCTTTGCGTTT
PRIMER_INTERNAL_30_SEQUENCE=CCAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_30=124,19
PRIMER_RIGHT_30=142,19
PRIMER_INTERNAL_30=121,23
PRIMER_LEFT_30_TM=62.194
PRIMER_RIGHT_30_TM=62.194
PRIMER_INTERNAL_30_TM=62.052
PRIMER_LEFT_30_GC_PERCENT=57.895
PRIMER_RIGHT_30_GC_PERCENT=57.895
PRIMER_INTERNAL_30_GC_PERCENT=60.870
PRIMER_INTERNAL_30_SELF_ANY_TH=0.00
PRIMER_LEFT_30_SELF_ANY_TH=0.00
PRIMER_RIGHT_30_SELF_ANY_TH=0.00
PRIMER_INTERNAL_30_SELF_END_TH=0.00
PRIMER_LEFT_30_SELF_END_TH=0.00
PRIMER_RIGHT_30_SELF_END_TH=0.00
PRIMER_LEFT_30_HAIRPIN_TH=0.00
PRIMER_RIGHT_30_HAIRPIN_TH=37.44
PRIMER_INTERNAL_30_HAIRPIN_TH=41.14
PRIMER_LEFT_30_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_30_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_30_END_STABILITY=4.3000
PRIMER_RIGHT_30_END_STABILITY=3.6000
PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=9.8734
PRIMER_LEFT_31_PENALTY=4.473143
PRIMER_RIGHT_31_PENALTY=4.473143
PRIMER_INTERNAL_31_PENALTY=5.152054
PRIMER_LEFT_31_SEQUENCE=TTCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_31_SEQUENCE=AGCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_31_SEQUENCE=TTCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_31=119,21
PRIMER_RIGHT_31=139,21
PRIMER_INTERNAL_31=119,24
PRIMER_LEFT_31_TM=62.473
PRIMER_RIGHT_31_TM=62.473
PRIMER_INTERNAL_31_TM=61.152
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_INTERNAL_31_GC_PERCENT=54.167
PRIMER_INTERNAL_31_SELF_ANY_TH=0.00
PRIMER_LEFT_31_SELF_ANY_TH=0.00
PRIMER_RIGHT_31_SELF_ANY_TH=0.00
PRIMER_INTERNAL_31_SELF_END_TH=0.00
PRIMER_LEFT_31_SELF_END_TH=0.00
PRIMER_RIGHT_31_SELF_END_TH=0.00
PRIMER_LEFT_31_HAIRPIN_TH=0.00
PRIMER_RIGHT_31_HAIRPIN_TH=37.44
PRIMER_INTERNAL_31_HAIRPIN_TH=0.00
PRIMER_LEFT_31_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_31_END_STABILITY=5.0700
PRIMER_RIGHT_31_END_STABILITY=3.5300
PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_32_PENALTY=4.837196
PRIMER_RIGHT_32_PENALTY=4.837196
PRIMER_INTERNAL_32_PENALTY=5.256814
PRIMER_LEFT_32_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_32_SEQUENCE=GAGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_32_SEQUENCE=CAAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_32=120,21
PRIMER_RIGHT_32=140,21
PRIMER_INTERNAL_32=122,24
PRIMER_LEFT_32_TM=62.837
PRIMER_RIGHT_32_TM=62.837
PRIMER_INTERNAL_32_TM=61.257
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_INTERNAL_32_GC_PERCENT=54.167
PRIMER_INTERNAL_32_SELF_ANY_TH=7.82
PRIMER_LEFT_32_SELF_ANY_TH=0.00
PRIMER_RIGHT_32_SELF_ANY_TH=0.00
PRIMER_INTERNAL_32_SELF_END_TH=0.00
PRIMER_LEFT_32_SELF_END_TH=0.00
PRIMER_RIGHT_32_SELF_END_TH=0.00
PRIMER_LEFT_32_HAIRPIN_TH=0.00
PRIMER_RIGHT_32_HAIRPIN_TH=37.44
PRIMER_INTERNAL_32_HAIRPIN_TH=36.42
PRIMER_LEFT_32_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_32_END_STABILITY=5.0300
PRIMER_RIGHT_32_END_STABILITY=3.5300
PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_INTERNAL_33_PENALTY=5.325970
PRIMER_INTERNAL_33_SEQUENCE=ATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_33=118,23
PRIMER_INTERNAL_33_TM=57.674
PRIMER_INTERNAL_33_GC_PERCENT=47.826
PRIMER_INTERNAL_33_SELF_ANY_TH=0.00
PRIMER_INTERNAL_33_SELF_END_TH=0.00
PRIMER_INTERNAL_33_HAIRPIN_TH=0.00
PRIMER_INTERNAL_34_PENALTY=5.931698
PRIMER_INTERNAL_34_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_34=117,25
PRIMER_INTERNAL_34_TM=59.068
PRIMER_INTERNAL_34_GC_PERCENT=48.000
PRIMER_INTERNAL_34_SELF_ANY_TH=0.00
PRIMER_INTERNAL_34_SELF_END_TH=0.00
PRIMER_INTERNAL_34_HAIRPIN_TH=0.00
PRIMER_INTERNAL_35_PENALTY=6.171494
PRIMER_INTERNAL_35_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_35=118,25
PRIMER_INTERNAL_35_TM=61.171
PRIMER_INTERNAL_35_GC_PERCENT=52.000
PRIMER_INTERNAL_35_SELF_ANY_TH=0.00
PRIMER_INTERNAL_35_SELF_END_TH=0.00
PRIMER_INTERNAL_35_HAIRPIN_TH=0.00
PRIMER_INTERNAL_36_PENALTY=6.354311
PRIMER_INTERNAL_36_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_36=116,26
PRIMER_INTERNAL_36_TM=59.646
PRIMER_INTERNAL_36_GC_PERCENT=50.000
PRIMER_INTERNAL_36_SELF_ANY_TH=0.00
PRIMER_INTERNAL_36_SELF_END_TH=0.00
PRIMER_INTERNAL_36_HAIRPIN_TH=0.00
PRIMER_INTERNAL_37_PENALTY=6.689016
PRIMER_INTERNAL_37_SEQUENCE=TATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_37=117,24
PRIMER_INTERNAL_37_TM=57.311
PRIMER_INTERNAL_37_GC_PERCENT=45.833
PRIMER_INTERNAL_37_SELF_ANY_TH=0.00
PRIMER_INTERNAL_37_SELF_END_TH=0.00
PRIMER_INTERNAL_37_HAIRPIN_TH=0.00
PRIMER_INTERNAL_38_PENALTY=6.689016
PRIMER_INTERNAL_38_SEQUENCE=TCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_38=115,24
PRIMER_INTERNAL_38_TM=57.311
PRIMER_INTERNAL_38_GC_PERCENT=45.833
PRIMER_INTERNAL_38_SELF_ANY_TH=0.00
PRIMER_INTERNAL_38_SELF_END_TH=0.00
PRIMER_INTERNAL_38_HAIRPIN_TH=0.00
PRIMER_INTERNAL_39_PENALTY=6.694952
PRIMER_INTERNAL_39_SEQUENCE=CTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_39=116,24
PRIMER_INTERNAL_39_TM=57.305
PRIMER_INTERNAL_39_GC_PERCENT=45.833
PRIMER_INTERNAL_39_SELF_ANY_TH=0.00
PRIMER_INTERNAL_39_SELF_END_TH=0.00
PRIMER_INTERNAL_39_HAIRPIN_TH=0.00
PRIMER_INTERNAL_40_PENALTY=6.707310
PRIMER_INTERNAL_40_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_40=117,26
PRIMER_INTERNAL_40_TM=60.707
PRIMER_INTERNAL_40_GC_PERCENT=50.000
PRIMER_INTERNAL_40_SELF_ANY_TH=0.00
PRIMER_INTERNAL_40_SELF_END_TH=0.00
PRIMER_INTERNAL_40_HAIRPIN_TH=0.00
PRIMER_INTERNAL_41_PENALTY=6.724062
PRIMER_INTERNAL_41_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_41=115,25
PRIMER_INTERNAL_41_TM=58.276
PRIMER_INTERNAL_41_GC_PERCENT=44.000
PRIMER_INTERNAL_41_SELF_ANY_TH=0.00
PRIMER_INTERNAL_41_SELF_END_TH=0.00
PRIMER_INTERNAL_41_HAIRPIN_TH=0.00
PRIMER_INTERNAL_42_PENALTY=6.744217
PRIMER_INTERNAL_42_SEQUENCE=CAAAACGCAAAGCACGCTCCCGATC
PRIMER_INTERNAL_42=122,25
PRIMER_INTERNAL_42_TM=61.744
PRIMER_INTERNAL_42_GC_PERCENT=56.000
PRIMER_INTERNAL_42_SELF_ANY_TH=7.82
PRIMER_INTERNAL_42_SELF_END_TH=0.00
PRIMER_INTERNAL_42_HAIRPIN_TH=36.42
PRIMER_INTERNAL_43_PENALTY=6.785590
PRIMER_INTERNAL_43_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_43=114,25
PRIMER_INTERNAL_43_TM=58.214
PRIMER_INTERNAL_43_GC_PERCENT=48.000
PRIMER_INTERNAL_43_SELF_ANY_TH=0.00
PRIMER_INTERNAL_43_SELF_END_TH=0.00
PRIMER_INTERNAL_43_HAIRPIN_TH=0.00
PRIMER_INTERNAL_44_PENALTY=6.889273
PRIMER_INTERNAL_44_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_44=114,26
PRIMER_INTERNAL_44_TM=59.111
PRIMER_INTERNAL_44_GC_PERCENT=46.154
PRIMER_INTERNAL_44_SELF_ANY_TH=0.00
PRIMER_INTERNAL_44_SELF_END_TH=0.00
PRIMER_INTERNAL_44_HAIRPIN_TH=0.00
PRIMER_INTERNAL_45_PENALTY=6.889273
PRIMER_INTERNAL_45_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_45=113,26
PRIMER_INTERNAL_45_TM=59.111
PRIMER_INTERNAL_45_GC_PERCENT=46.154
PRIMER_INTERNAL_45_SELF_ANY_TH=0.00
PRIMER_INTERNAL_45_SELF_END_TH=0.00
PRIMER_INTERNAL_45_HAIRPIN_TH=0.00
PRIMER_INTERNAL_46_PENALTY=6.910113
PRIMER_INTERNAL_46_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGA
PRIMER_INTERNAL_46=121,24
PRIMER_INTERNAL_46_TM=62.910
PRIMER_INTERNAL_46_GC_PERCENT=58.333
PRIMER_INTERNAL_46_SELF_ANY_TH=7.82
PRIMER_INTERNAL_46_SELF_END_TH=0.00
PRIMER_INTERNAL_46_HAIRPIN_TH=36.42
PRIMER_INTERNAL_47_PENALTY=6.910113
PRIMER_INTERNAL_47_SEQUENCE=TCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_47=120,24
PRIMER_INTERNAL_47_TM=62.910
PRIMER_INTERNAL_47_GC_PERCENT=58.333
PRIMER_INTERNAL_47_SELF_ANY_TH=0.00
PRIMER_INTERNAL_47_SELF_END_TH=0.00
PRIMER_INTERNAL_47_HAIRPIN_TH=41.14
PRIMER_INTERNAL_48_PENALTY=7.008353
PRIMER_INTERNAL_48_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_48=116,25
PRIMER_INTERNAL_48_TM=57.992
PRIMER_INTERNAL_48_GC_PERCENT=48.000
PRIMER_INTERNAL_48_SELF_ANY_TH=0.00
PRIMER_INTERNAL_48_SELF_END_TH=0.00
PRIMER_INTERNAL_48_HAIRPIN_TH=0.00
PRIMER_INTERNAL_49_PENALTY=7.035358
PRIMER_INTERNAL_49_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_49=113,27
PRIMER_INTERNAL_49_TM=59.965
PRIMER_INTERNAL_49_GC_PERCENT=44.444
PRIMER_INTERNAL_49_SELF_ANY_TH=0.00
PRIMER_INTERNAL_49_SELF_END_TH=0.00
PRIMER_INTERNAL_49_HAIRPIN_TH=0.00
PRIMER_INTERNAL_50_PENALTY=7.102160
PRIMER_INTERNAL_50_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_50=115,26
PRIMER_INTERNAL_50_TM=58.898
PRIMER_INTERNAL_50_GC_PERCENT=46.154
PRIMER_INTERNAL_50_SELF_ANY_TH=0.00
PRIMER_INTERNAL_50_SELF_END_TH=0.00
PRIMER_INTERNAL_50_HAIRPIN_TH=0.00
PRIMER_INTERNAL_51_PENALTY=7.334450
PRIMER_INTERNAL_51_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_51=114,27
PRIMER_INTERNAL_51_TM=59.666
PRIMER_INTERNAL_51_GC_PERCENT=48.148
PRIMER_INTERNAL_51_SELF_ANY_TH=0.00
PRIMER_INTERNAL_51_SELF_END_TH=0.00
PRIMER_INTERNAL_51_HAIRPIN_TH=0.00
PRIMER_INTERNAL_52_PENALTY=7.480679
PRIMER_INTERNAL_52_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_52=115,27
PRIMER_INTERNAL_52_TM=60.481
PRIMER_INTERNAL_52_GC_PERCENT=48.148
PRIMER_INTERNAL_52_SELF_ANY_TH=0.00
PRIMER_INTERNAL_52_SELF_END_TH=0.00
PRIMER_INTERNAL_52_HAIRPIN_TH=0.00
PRIMER_INTERNAL_53_PENALTY=7.647244
PRIMER_INTERNAL_53_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_53=112,27
PRIMER_INTERNAL_53_TM=59.353
PRIMER_INTERNAL_53_GC_PERCENT=44.444
PRIMER_INTERNAL_53_SELF_ANY_TH=0.00
PRIMER_INTERNAL_53_SELF_END_TH=0.00
PRIMER_INTERNAL_53_HAIRPIN_TH=0.00
PRIMER_INTERNAL_54_PENALTY=7.744113
PRIMER_INTERNAL_54_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_54=55,26
PRIMER_INTERNAL_54_TM=58.256
PRIMER_INTERNAL_54_GC_PERCENT=46.154
PRIMER_INTERNAL_54_SELF_ANY_TH=0.00
PRIMER_INTERNAL_54_SELF_END_TH=0.00
PRIMER_INTERNAL_54_HAIRPIN_TH=0.00
PRIMER_INTERNAL_55_PENALTY=7.867066
PRIMER_INTERNAL_55_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGAT
PRIMER_INTERNAL_55=121,25
PRIMER_INTERNAL_55_TM=62.867
PRIMER_INTERNAL_55_GC_PERCENT=56.000
PRIMER_INTERNAL_55_SELF_ANY_TH=7.82
PRIMER_INTERNAL_55_SELF_END_TH=0.00
PRIMER_INTERNAL_55_HAIRPIN_TH=36.42
PRIMER_INTERNAL_56_PENALTY=7.916609
PRIMER_INTERNAL_56_SEQUENCE=TGACCTGATAGAAACGCCAACTCAC
PRIMER_INTERNAL_56=53,25
PRIMER_INTERNAL_56_TM=57.083
PRIMER_INTERNAL_56_GC_PERCENT=48.000
PRIMER_INTERNAL_56_SELF_ANY_TH=0.00
PRIMER_INTERNAL_56_SELF_END_TH=0.00
PRIMER_INTERNAL_56_HAIRPIN_TH=0.00
PRIMER_INTERNAL_57_PENALTY=7.922893
PRIMER_INTERNAL_57_SEQUENCE=CCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_57=56,25
PRIMER_INTERNAL_57_TM=57.077
PRIMER_INTERNAL_57_GC_PERCENT=48.000
PRIMER_INTERNAL_57_SELF_ANY_TH=0.00
PRIMER_INTERNAL_57_SELF_END_TH=0.00
PRIMER_INTERNAL_57_HAIRPIN_TH=0.00
PRIMER_INTERNAL_58_PENALTY=7.922893
PRIMER_INTERNAL_58_SEQUENCE=ACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_58=55,25
PRIMER_INTERNAL_58_TM=57.077
PRIMER_INTERNAL_58_GC_PERCENT=48.000
PRIMER_INTERNAL_58_SELF_ANY_TH=0.00
PRIMER_INTERNAL_58_SELF_END_TH=0.00
PRIMER_INTERNAL_58_HAIRPIN_TH=0.00
PRIMER_INTERNAL_59_PENALTY=7.963894
PRIMER_INTERNAL_59_SEQUENCE=TGACCTGATAGAAACGCCAACTCACT
PRIMER_INTERNAL_59=53,26
PRIMER_INTERNAL_59_TM=58.036
PRIMER_INTERNAL_59_GC_PERCENT=46.154
PRIMER_INTERNAL_59_SELF_ANY_TH=0.00
PRIMER_INTERNAL_59_SELF_END_TH=0.00
PRIMER_INTERNAL_59_HAIRPIN_TH=0.00
PRIMER_INTERNAL_60_PENALTY=8.131843
PRIMER_INTERNAL_60_SEQUENCE=TGACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_60=53,27
PRIMER_INTERNAL_60_TM=58.868
PRIMER_INTERNAL_60_GC_PERCENT=48.148
PRIMER_INTERNAL_60_SELF_ANY_TH=0.00
PRIMER_INTERNAL_60_SELF_END_TH=0.00
PRIMER_INTERNAL_60_HAIRPIN_TH=0.00
PRIMER_INTERNAL_61_PENALTY=8.132460
PRIMER_INTERNAL_61_SEQUENCE=GACCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_61=54,27
PRIMER_INTERNAL_61_TM=58.868
PRIMER_INTERNAL_61_GC_PERCENT=48.148
PRIMER_INTERNAL_61_SELF_ANY_TH=0.00
PRIMER_INTERNAL_61_SELF_END_TH=0.00
PRIMER_INTERNAL_61_HAIRPIN_TH=0.00
PRIMER_INTERNAL_62_PENALTY=8.193320
PRIMER_INTERNAL_62_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_62=116,27
PRIMER_INTERNAL_62_TM=61.193
PRIMER_INTERNAL_62_GC_PERCENT=51.852
PRIMER_INTERNAL_62_SELF_ANY_TH=0.00
PRIMER_INTERNAL_62_SELF_END_TH=0.00
PRIMER_INTERNAL_62_HAIRPIN_TH=0.00
PRIMER_INTERNAL_63_PENALTY=8.237701
PRIMER_INTERNAL_63_SEQUENCE=GACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_63=54,26
PRIMER_INTERNAL_63_TM=57.762
PRIMER_INTERNAL_63_GC_PERCENT=50.000
PRIMER_INTERNAL_63_SELF_ANY_TH=0.00
PRIMER_INTERNAL_63_SELF_END_TH=0.00
PRIMER_INTERNAL_63_HAIRPIN_TH=0.00
PRIMER_INTERNAL_64_PENALTY=8.755267
PRIMER_INTERNAL_64_SEQUENCE=ATGACCTGATAGAAACGCCAACTCAC
PRIMER_INTERNAL_64=52,26
PRIMER_INTERNAL_64_TM=57.245
PRIMER_INTERNAL_64_GC_PERCENT=46.154
PRIMER_INTERNAL_64_SELF_ANY_TH=0.00
PRIMER_INTERNAL_64_SELF_END_TH=0.00
PRIMER_INTERNAL_64_HAIRPIN_TH=0.00
PRIMER_INTERNAL_65_PENALTY=8.758760
PRIMER_INTERNAL_65_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACG
PRIMER_INTERNAL_65=112,26
PRIMER_INTERNAL_65_TM=57.241
PRIMER_INTERNAL_65_GC_PERCENT=42.308
PRIMER_INTERNAL_65_SELF_ANY_TH=0.00
PRIMER_INTERNAL_65_SELF_END_TH=0.00
PRIMER_INTERNAL_65_HAIRPIN_TH=0.00
PRIMER_INTERNAL_66_PENALTY=8.838967
PRIMER_INTERNAL_66_SEQUENCE=ATGACCTGATAGAAACGCCAACTCACT
PRIMER_INTERNAL_66=52,27
PRIMER_INTERNAL_66_TM=58.161
PRIMER_INTERNAL_66_GC_PERCENT=44.444
PRIMER_INTERNAL_66_SELF_ANY_TH=0.00
PRIMER_INTERNAL_66_SELF_END_TH=0.00
PRIMER_INTERNAL_66_HAIRPIN_TH=0.00
PRIMER_INTERNAL_67_PENALTY=8.978186
PRIMER_INTERNAL_67_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_67=118,26
PRIMER_INTERNAL_67_TM=62.978
PRIMER_INTERNAL_67_GC_PERCENT=53.846
PRIMER_INTERNAL_67_SELF_ANY_TH=0.00
PRIMER_INTERNAL_67_SELF_END_TH=0.00
PRIMER_INTERNAL_67_HAIRPIN_TH=41.14
PRIMER_INTERNAL_68_PENALTY=9.099564
PRIMER_INTERNAL_68_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGTA
PRIMER_INTERNAL_68=55,27
PRIMER_INTERNAL_68_TM=57.900
PRIMER_INTERNAL_68_GC_PERCENT=44.444
PRIMER_INTERNAL_68_SELF_ANY_TH=0.00
PRIMER_INTERNAL_68_SELF_END_TH=0.00
PRIMER_INTERNAL_68_HAIRPIN_TH=0.00
PRIMER_INTERNAL_69_PENALTY=9.448747
PRIMER_INTERNAL_69_SEQUENCE=AAAGTCTATTCCAAAACGCAAAGCACG
PRIMER_INTERNAL_69=111,27
PRIMER_INTERNAL_69_TM=57.551
PRIMER_INTERNAL_69_GC_PERCENT=40.741
PRIMER_INTERNAL_69_SELF_ANY_TH=0.00
PRIMER_INTERNAL_69_SELF_END_TH=0.00
PRIMER_INTERNAL_69_HAIRPIN_TH=0.00
PRIMER_INTERNAL_70_PENALTY=9.472089
PRIMER_INTERNAL_70_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_70=117,27
PRIMER_INTERNAL_70_TM=62.472
PRIMER_INTERNAL_70_GC_PERCENT=51.852
PRIMER_INTERNAL_70_SELF_ANY_TH=0.00
PRIMER_INTERNAL_70_SELF_END_TH=0.00
PRIMER_INTERNAL_70_HAIRPIN_TH=41.14
PRIMER_INTERNAL_71_PENALTY=9.912698
PRIMER_INTERNAL_71_SEQUENCE=CGTTGTCAAAGTCTATTCCAAAACGCA
PRIMER_INTERNAL_71=104,27
PRIMER_INTERNAL_71_TM=57.087
PRIMER_INTERNAL_71_GC_PERCENT=40.741
PRIMER_INTERNAL_71_SELF_ANY_TH=0.00
PRIMER_INTERNAL_71_SELF_END_TH=0.00
PRIMER_INTERNAL_71_HAIRPIN_TH=26.69
PRIMER_INTERNAL_72_PENALTY=9.914131
PRIMER_INTERNAL_72_SEQUENCE=ACGTTGTCAAAGTCTATTCCAAAACGC
PRIMER_INTERNAL_72=103,27
PRIMER_INTERNAL_72_TM=57.086
PRIMER_INTERNAL_72_GC_PERCENT=40.741
PRIMER_INTERNAL_72_SELF_ANY_TH=0.00
PRIMER_INTERNAL_72_SELF_END_TH=0.00
PRIMER_INTERNAL_72_HAIRPIN_TH=35.24
=
PRIMER_TASK=pick_primer_list
PRIMER_PRODUCT_SIZE_RANGE=75-200
P3_FILE_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=4
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=4
SEQUENCE_ID=pick_primer_list_and_file_flag_th_3
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52
PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 17, high repeat similarity 3, ok 34
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=52
PRIMER_RIGHT_NUM_RETURNED=34
PRIMER_INTERNAL_NUM_RETURNED=168
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.527529
PRIMER_RIGHT_0_PENALTY=0.527529
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=80,20
PRIMER_INTERNAL_0=66,20
PRIMER_LEFT_0_TM=59.528
PRIMER_RIGHT_0_TM=59.528
PRIMER_INTERNAL_0_TM=59.273
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_LEFT_0_SELF_ANY_TH=10.70
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=40.47
PRIMER_RIGHT_0_HAIRPIN_TH=41.64
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_RIGHT_0_END_STABILITY=2.2400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266
PRIMER_LEFT_1_PENALTY=0.606923
PRIMER_RIGHT_1_PENALTY=0.606923
PRIMER_INTERNAL_1_PENALTY=1.416589
PRIMER_LEFT_1_SEQUENCE=CCCGACCCAAATCGATAGCT
PRIMER_RIGHT_1_SEQUENCE=AGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC
PRIMER_LEFT_1=78,20
PRIMER_RIGHT_1=97,20
PRIMER_INTERNAL_1=64,21
PRIMER_LEFT_1_TM=59.607
PRIMER_RIGHT_1_TM=59.607
PRIMER_INTERNAL_1_TM=59.583
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_SELF_ANY_TH=7.03
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=1.71
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.13
PRIMER_RIGHT_1_HAIRPIN_TH=41.74
PRIMER_INTERNAL_1_HAIRPIN_TH=41.60
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_1_END_STABILITY=3.3200
PRIMER_RIGHT_1_END_STABILITY=5.1400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_2_PENALTY=0.608403
PRIMER_RIGHT_2_PENALTY=0.608403
PRIMER_INTERNAL_2_PENALTY=1.438741
PRIMER_LEFT_2_SEQUENCE=TCCCGACCCAAATCGATAGC
PRIMER_RIGHT_2_SEQUENCE=GCTATCGATTTGGGTCGGGA
PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA
PRIMER_LEFT_2=77,20
PRIMER_RIGHT_2=96,20
PRIMER_INTERNAL_2=67,20
PRIMER_LEFT_2_TM=59.608
PRIMER_RIGHT_2_TM=59.608
PRIMER_INTERNAL_2_TM=58.561
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=1.71
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=45.13
PRIMER_RIGHT_2_HAIRPIN_TH=41.74
PRIMER_INTERNAL_2_HAIRPIN_TH=41.60
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_2_END_STABILITY=2.9700
PRIMER_RIGHT_2_END_STABILITY=5.1400
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=2.4428
PRIMER_LEFT_3_PENALTY=0.713597
PRIMER_RIGHT_3_PENALTY=0.713597
PRIMER_INTERNAL_3_PENALTY=1.438741
PRIMER_LEFT_3_SEQUENCE=GACATGACACTGACGATGCA
PRIMER_RIGHT_3_SEQUENCE=TGCATCGTCAGTGTCATGTC
PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC
PRIMER_LEFT_3=11,20
PRIMER_RIGHT_3=30,20
PRIMER_INTERNAL_3=65,20
PRIMER_LEFT_3_TM=58.286
PRIMER_RIGHT_3_TM=58.286
PRIMER_INTERNAL_3_TM=58.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=41.60
PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_3_END_STABILITY=3.9600
PRIMER_RIGHT_3_END_STABILITY=3.0600
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=16.7219
PRIMER_LEFT_4_PENALTY=0.966496
PRIMER_RIGHT_4_PENALTY=0.966496
PRIMER_INTERNAL_4_PENALTY=1.650306
PRIMER_LEFT_4_SEQUENCE=CGATCCCGACCCAAATCGAT
PRIMER_RIGHT_4_SEQUENCE=ATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA
PRIMER_LEFT_4=74,20
PRIMER_RIGHT_4=93,20
PRIMER_INTERNAL_4=66,21
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.966
PRIMER_INTERNAL_4_TM=60.650
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_INTERNAL_4_SELF_ANY_TH=2.18
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=45.13
PRIMER_RIGHT_4_HAIRPIN_TH=41.74
PRIMER_INTERNAL_4_HAIRPIN_TH=41.60
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_4_END_STABILITY=3.5900
PRIMER_RIGHT_4_END_STABILITY=3.6900
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_5_PENALTY=0.967319
PRIMER_RIGHT_5_PENALTY=1.001812
PRIMER_INTERNAL_5_PENALTY=1.650306
PRIMER_LEFT_5_SEQUENCE=CATTCGATCCCGACCCAAAT
PRIMER_RIGHT_5_SEQUENCE=GTCTGCATCGTCAGTGTCAT
PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC
PRIMER_LEFT_5=70,20
PRIMER_RIGHT_5=33,20
PRIMER_INTERNAL_5=65,21
PRIMER_LEFT_5_TM=58.033
PRIMER_RIGHT_5_TM=57.998
PRIMER_INTERNAL_5_TM=60.650
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_INTERNAL_5_GC_PERCENT=66.667
PRIMER_INTERNAL_5_SELF_ANY_TH=0.00
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=45.50
PRIMER_RIGHT_5_HAIRPIN_TH=35.63
PRIMER_INTERNAL_5_HAIRPIN_TH=41.60
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_5_END_STABILITY=2.3200
PRIMER_RIGHT_5_END_STABILITY=3.0600
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=8.6039
PRIMER_LEFT_6_PENALTY=1.000429
PRIMER_RIGHT_6_PENALTY=1.001812
PRIMER_INTERNAL_6_PENALTY=2.095978
PRIMER_LEFT_6_SEQUENCE=CATGACACTGACGATGCAGA
PRIMER_RIGHT_6_SEQUENCE=GCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA
PRIMER_LEFT_6=13,20
PRIMER_RIGHT_6=29,20
PRIMER_INTERNAL_6=67,21
PRIMER_LEFT_6_TM=58.000
PRIMER_RIGHT_6_TM=57.998
PRIMER_INTERNAL_6_TM=58.904
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=0.00
PRIMER_LEFT_6_SELF_ANY_TH=10.65
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=39.20
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=41.60
PRIMER_LEFT_6_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_6_END_STABILITY=4.2600
PRIMER_RIGHT_6_END_STABILITY=3.4100
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=1.2632
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_7_PENALTY=1.001812
PRIMER_RIGHT_7_PENALTY=1.142220
PRIMER_INTERNAL_7_PENALTY=2.360266
PRIMER_LEFT_7_SEQUENCE=ATGACACTGACGATGCAGAC
PRIMER_RIGHT_7_SEQUENCE=GGGATCGAATGGCCCACTA
PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_7=14,20
PRIMER_RIGHT_7=80,19
PRIMER_INTERNAL_7=63,21
PRIMER_LEFT_7_TM=57.998
PRIMER_RIGHT_7_TM=58.858
PRIMER_INTERNAL_7_TM=58.640
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=57.895
PRIMER_INTERNAL_7_GC_PERCENT=61.905
PRIMER_INTERNAL_7_SELF_ANY_TH=7.03
PRIMER_LEFT_7_SELF_ANY_TH=10.65
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=39.20
PRIMER_RIGHT_7_HAIRPIN_TH=41.64
PRIMER_INTERNAL_7_HAIRPIN_TH=41.60
PRIMER_LEFT_7_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_7_END_STABILITY=3.5100
PRIMER_RIGHT_7_END_STABILITY=2.7400
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=16.1639
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=7.8928
PRIMER_LEFT_8_PENALTY=1.001812
PRIMER_RIGHT_8_PENALTY=1.316792
PRIMER_INTERNAL_8_PENALTY=2.456420
PRIMER_LEFT_8_SEQUENCE=AGACATGACACTGACGATGC
PRIMER_RIGHT_8_SEQUENCE=CGTAGCTATCGATTTGGGTCG
PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA
PRIMER_LEFT_8=10,20
PRIMER_RIGHT_8=100,21
PRIMER_INTERNAL_8=63,20
PRIMER_LEFT_8_TM=57.998
PRIMER_RIGHT_8_TM=58.683
PRIMER_INTERNAL_8_TM=57.544
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_INTERNAL_8_SELF_ANY_TH=7.03
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=1.71
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=42.74
PRIMER_INTERNAL_8_HAIRPIN_TH=39.49
PRIMER_LEFT_8_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_8_END_STABILITY=3.9100
PRIMER_RIGHT_8_END_STABILITY=4.7900
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=7.5573
PRIMER_LEFT_9_PENALTY=1.107410
PRIMER_RIGHT_9_PENALTY=1.383135
PRIMER_INTERNAL_9_PENALTY=2.511372
PRIMER_LEFT_9_SEQUENCE=CCATTCGATCCCGACCCAAA
PRIMER_RIGHT_9_SEQUENCE=TAGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC
PRIMER_LEFT_9=69,20
PRIMER_RIGHT_9=98,21
PRIMER_INTERNAL_9=64,20
PRIMER_LEFT_9_TM=60.107
PRIMER_RIGHT_9_TM=59.383
PRIMER_INTERNAL_9_TM=57.489
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_INTERNAL_9_GC_PERCENT=65.000
PRIMER_INTERNAL_9_SELF_ANY_TH=7.03
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=1.71
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=45.50
PRIMER_RIGHT_9_HAIRPIN_TH=41.74
PRIMER_INTERNAL_9_HAIRPIN_TH=41.60
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_9_END_STABILITY=3.2800
PRIMER_RIGHT_9_END_STABILITY=5.1400
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_10_PENALTY=1.126409
PRIMER_RIGHT_10_PENALTY=1.559940
PRIMER_INTERNAL_10_PENALTY=2.626619
PRIMER_LEFT_10_SEQUENCE=CATTCGATCCCGACCCAAATC
PRIMER_RIGHT_10_SEQUENCE=TCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_10=70,21
PRIMER_RIGHT_10=92,19
PRIMER_INTERNAL_10=63,22
PRIMER_LEFT_10_TM=59.126
PRIMER_RIGHT_10_TM=59.560
PRIMER_INTERNAL_10_TM=60.627
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=57.895
PRIMER_INTERNAL_10_GC_PERCENT=63.636
PRIMER_INTERNAL_10_SELF_ANY_TH=7.03
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=45.50
PRIMER_RIGHT_10_HAIRPIN_TH=41.74
PRIMER_INTERNAL_10_HAIRPIN_TH=41.60
PRIMER_LEFT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_10_END_STABILITY=2.1700
PRIMER_RIGHT_10_END_STABILITY=3.6900
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_11_PENALTY=1.142220
PRIMER_RIGHT_11_PENALTY=1.559940
PRIMER_INTERNAL_11_PENALTY=2.783921
PRIMER_LEFT_11_SEQUENCE=TAGTGGGCCATTCGATCCC
PRIMER_RIGHT_11_SEQUENCE=CGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_11=62,19
PRIMER_RIGHT_11=91,19
PRIMER_INTERNAL_11=67,22
PRIMER_LEFT_11_TM=58.858
PRIMER_RIGHT_11_TM=59.560
PRIMER_INTERNAL_11_TM=59.216
PRIMER_LEFT_11_GC_PERCENT=57.895
PRIMER_RIGHT_11_GC_PERCENT=57.895
PRIMER_INTERNAL_11_GC_PERCENT=59.091
PRIMER_INTERNAL_11_SELF_ANY_TH=0.00
PRIMER_LEFT_11_SELF_ANY_TH=10.70
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=40.47
PRIMER_RIGHT_11_HAIRPIN_TH=42.82
PRIMER_INTERNAL_11_HAIRPIN_TH=41.60
PRIMER_LEFT_11_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_11_END_STABILITY=3.8500
PRIMER_RIGHT_11_END_STABILITY=3.5900
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_12_PENALTY=1.179094
PRIMER_RIGHT_12_PENALTY=1.568623
PRIMER_INTERNAL_12_PENALTY=2.885177
PRIMER_LEFT_12_SEQUENCE=TTCGATCCCGACCCAAATCG
PRIMER_RIGHT_12_SEQUENCE=GGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA
PRIMER_LEFT_12=72,20
PRIMER_RIGHT_12=79,20
PRIMER_INTERNAL_12=66,22
PRIMER_LEFT_12_TM=60.179
PRIMER_RIGHT_12_TM=57.431
PRIMER_INTERNAL_12_TM=60.885
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=63.636
PRIMER_INTERNAL_12_SELF_ANY_TH=2.18
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=45.50
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.60
PRIMER_LEFT_12_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_12_END_STABILITY=3.3400
PRIMER_RIGHT_12_END_STABILITY=1.7300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=2.4855
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=4.2003
PRIMER_LEFT_13_PENALTY=1.256117
PRIMER_RIGHT_13_PENALTY=1.673833
PRIMER_INTERNAL_13_PENALTY=3.114431
PRIMER_LEFT_13_SEQUENCE=ATTCGATCCCGACCCAAATC
PRIMER_RIGHT_13_SEQUENCE=GTAGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_13=71,20
PRIMER_RIGHT_13=99,21
PRIMER_INTERNAL_13=62,23
PRIMER_LEFT_13_TM=57.744
PRIMER_RIGHT_13_TM=58.326
PRIMER_INTERNAL_13_TM=60.114
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_INTERNAL_13_GC_PERCENT=60.870
PRIMER_INTERNAL_13_SELF_ANY_TH=7.03
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=1.71
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=45.50
PRIMER_RIGHT_13_HAIRPIN_TH=41.74
PRIMER_INTERNAL_13_HAIRPIN_TH=41.60
PRIMER_LEFT_13_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_13_END_STABILITY=2.1700
PRIMER_RIGHT_13_END_STABILITY=4.7900
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=2.2809
PRIMER_LEFT_14_PENALTY=1.316792
PRIMER_RIGHT_14_PENALTY=1.726673
PRIMER_INTERNAL_14_PENALTY=3.510595
PRIMER_LEFT_14_SEQUENCE=CGACCCAAATCGATAGCTACG
PRIMER_RIGHT_14_SEQUENCE=TGCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC
PRIMER_LEFT_14=80,21
PRIMER_RIGHT_14=30,21
PRIMER_INTERNAL_14=64,22
PRIMER_LEFT_14_TM=58.683
PRIMER_RIGHT_14_TM=59.727
PRIMER_INTERNAL_14_TM=61.511
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_INTERNAL_14_GC_PERCENT=68.182
PRIMER_INTERNAL_14_SELF_ANY_TH=7.03
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=2.30
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=45.13
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=41.60
PRIMER_LEFT_14_LIBRARY_MISPRIMING=10.00, MER4A a consensus
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_14_END_STABILITY=3.5100
PRIMER_RIGHT_14_END_STABILITY=3.4100
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5113
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_15_PENALTY=1.383135
PRIMER_RIGHT_15_PENALTY=1.728621
PRIMER_INTERNAL_15_PENALTY=3.685489
PRIMER_LEFT_15_SEQUENCE=CCCGACCCAAATCGATAGCTA
PRIMER_RIGHT_15_SEQUENCE=TATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_15=78,21
PRIMER_RIGHT_15=94,21
PRIMER_INTERNAL_15=67,23
PRIMER_LEFT_15_TM=59.383
PRIMER_RIGHT_15_TM=59.729
PRIMER_INTERNAL_15_TM=59.315
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_INTERNAL_15_GC_PERCENT=56.522
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=1.71
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=45.13
PRIMER_RIGHT_15_HAIRPIN_TH=41.74
PRIMER_INTERNAL_15_HAIRPIN_TH=41.60
PRIMER_LEFT_15_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_15_END_STABILITY=3.3200
PRIMER_RIGHT_15_END_STABILITY=3.6900
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=13.2273
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_16_PENALTY=1.475039
PRIMER_RIGHT_16_PENALTY=1.914379
PRIMER_INTERNAL_16_PENALTY=3.807618
PRIMER_LEFT_16_SEQUENCE=CCATTCGATCCCGACCCAA
PRIMER_RIGHT_16_SEQUENCE=GCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_16=69,19
PRIMER_RIGHT_16=96,19
PRIMER_INTERNAL_16=62,22
PRIMER_LEFT_16_TM=59.475
PRIMER_RIGHT_16_TM=58.086
PRIMER_INTERNAL_16_TM=58.192
PRIMER_LEFT_16_GC_PERCENT=57.895
PRIMER_RIGHT_16_GC_PERCENT=57.895
PRIMER_INTERNAL_16_GC_PERCENT=59.091
PRIMER_INTERNAL_16_SELF_ANY_TH=7.03
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=1.71
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=45.50
PRIMER_RIGHT_16_HAIRPIN_TH=41.74
PRIMER_INTERNAL_16_HAIRPIN_TH=41.60
PRIMER_LEFT_16_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_16_END_STABILITY=4.1200
PRIMER_RIGHT_16_END_STABILITY=5.1400
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_17_PENALTY=1.559940
PRIMER_RIGHT_17_PENALTY=1.926077
PRIMER_INTERNAL_17_PENALTY=3.826170
PRIMER_LEFT_17_SEQUENCE=CGATCCCGACCCAAATCGA
PRIMER_RIGHT_17_SEQUENCE=GGGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA
PRIMER_LEFT_17=74,19
PRIMER_RIGHT_17=80,21
PRIMER_INTERNAL_17=68,21
PRIMER_LEFT_17_TM=59.560
PRIMER_RIGHT_17_TM=59.926
PRIMER_INTERNAL_17_TM=57.174
PRIMER_LEFT_17_GC_PERCENT=57.895
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=45.13
PRIMER_RIGHT_17_HAIRPIN_TH=41.64
PRIMER_INTERNAL_17_HAIRPIN_TH=41.60
PRIMER_LEFT_17_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_17_END_STABILITY=3.5900
PRIMER_RIGHT_17_END_STABILITY=1.7300
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=12.3668
PRIMER_LEFT_18_PENALTY=1.559940
PRIMER_RIGHT_18_PENALTY=1.960819
PRIMER_INTERNAL_18_PENALTY=3.864865
PRIMER_LEFT_18_SEQUENCE=TCGATCCCGACCCAAATCG
PRIMER_RIGHT_18_SEQUENCE=CTATCGATTTGGGTCGGGATC
PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT
PRIMER_LEFT_18=73,19
PRIMER_RIGHT_18=95,21
PRIMER_INTERNAL_18=37,23
PRIMER_LEFT_18_TM=59.560
PRIMER_RIGHT_18_TM=58.039
PRIMER_INTERNAL_18_TM=59.135
PRIMER_LEFT_18_GC_PERCENT=57.895
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_INTERNAL_18_GC_PERCENT=52.174
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=1.71
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=40.30
PRIMER_RIGHT_18_HAIRPIN_TH=41.74
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_18_END_STABILITY=3.3400
PRIMER_RIGHT_18_END_STABILITY=3.3600
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=0.0363
PRIMER_LEFT_19_PENALTY=1.568623
PRIMER_RIGHT_19_PENALTY=1.997913
PRIMER_INTERNAL_19_PENALTY=3.881423
PRIMER_LEFT_19_SEQUENCE=ATTAGTGGGCCATTCGATCC
PRIMER_RIGHT_19_SEQUENCE=GCTATCGATTTGGGTCGGGAT
PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_19=60,20
PRIMER_RIGHT_19=96,21
PRIMER_INTERNAL_19=62,21
PRIMER_LEFT_19_TM=57.431
PRIMER_RIGHT_19_TM=59.998
PRIMER_INTERNAL_19_TM=57.119
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_INTERNAL_19_GC_PERCENT=57.143
PRIMER_INTERNAL_19_SELF_ANY_TH=7.03
PRIMER_LEFT_19_SELF_ANY_TH=10.70
PRIMER_RIGHT_19_SELF_ANY_TH=1.71
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=41.74
PRIMER_INTERNAL_19_HAIRPIN_TH=39.49
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_19_END_STABILITY=3.3600
PRIMER_RIGHT_19_END_STABILITY=3.8500
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=9.4710
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=1.673833
PRIMER_RIGHT_20_PENALTY=2.038681
PRIMER_INTERNAL_20_PENALTY=3.936624
PRIMER_LEFT_20_SEQUENCE=CCGACCCAAATCGATAGCTAC
PRIMER_RIGHT_20_SEQUENCE=TCGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA
PRIMER_LEFT_20=79,21
PRIMER_RIGHT_20=92,20
PRIMER_INTERNAL_20=65,22
PRIMER_LEFT_20_TM=58.326
PRIMER_RIGHT_20_TM=61.039
PRIMER_INTERNAL_20_TM=61.937
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_INTERNAL_20_GC_PERCENT=63.636
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=3.47
PRIMER_INTERNAL_20_SELF_END_TH=3.99
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=3.47
PRIMER_LEFT_20_HAIRPIN_TH=45.13
PRIMER_RIGHT_20_HAIRPIN_TH=45.48
PRIMER_INTERNAL_20_HAIRPIN_TH=42.67
PRIMER_LEFT_20_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_20_END_STABILITY=3.5800
PRIMER_RIGHT_20_END_STABILITY=3.5900
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.3824
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_21_PENALTY=1.726673
PRIMER_RIGHT_21_PENALTY=2.150848
PRIMER_INTERNAL_21_PENALTY=3.956741
PRIMER_LEFT_21_SEQUENCE=AGACATGACACTGACGATGCA
PRIMER_RIGHT_21_SEQUENCE=GCATCGTCAGTGTCATGTCTA
PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_LEFT_21=10,21
PRIMER_RIGHT_21=29,21
PRIMER_INTERNAL_21=67,19
PRIMER_LEFT_21_TM=59.727
PRIMER_RIGHT_21_TM=57.849
PRIMER_INTERNAL_21_TM=57.043
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_INTERNAL_21_GC_PERCENT=68.421
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.60
PRIMER_LEFT_21_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_21_END_STABILITY=3.9600
PRIMER_RIGHT_21_END_STABILITY=2.5900
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=9.1785
PRIMER_LEFT_22_PENALTY=1.728621
PRIMER_RIGHT_22_PENALTY=2.496898
PRIMER_INTERNAL_22_PENALTY=3.956741
PRIMER_LEFT_22_SEQUENCE=CGATCCCGACCCAAATCGATA
PRIMER_RIGHT_22_SEQUENCE=GTCTGCATCGTCAGTGTCA
PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_LEFT_22=74,21
PRIMER_RIGHT_22=33,19
PRIMER_INTERNAL_22=66,19
PRIMER_LEFT_22_TM=59.729
PRIMER_RIGHT_22_TM=57.503
PRIMER_INTERNAL_22_TM=57.043
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=52.632
PRIMER_INTERNAL_22_GC_PERCENT=68.421
PRIMER_INTERNAL_22_SELF_ANY_TH=2.18
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=45.13
PRIMER_RIGHT_22_HAIRPIN_TH=35.63
PRIMER_INTERNAL_22_HAIRPIN_TH=41.60
PRIMER_LEFT_22_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_22_END_STABILITY=2.9200
PRIMER_RIGHT_22_END_STABILITY=3.5800
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=6.6045
PRIMER_LEFT_23_PENALTY=1.914379
PRIMER_RIGHT_23_PENALTY=2.637998
PRIMER_INTERNAL_23_PENALTY=4.001118
PRIMER_LEFT_23_SEQUENCE=CCCGACCCAAATCGATAGC
PRIMER_RIGHT_23_SEQUENCE=TCGTAGCTATCGATTTGGGTC
PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT
PRIMER_LEFT_23=78,19
PRIMER_RIGHT_23=101,21
PRIMER_INTERNAL_23=36,24
PRIMER_LEFT_23_TM=58.086
PRIMER_RIGHT_23_TM=57.362
PRIMER_INTERNAL_23_TM=60.001
PRIMER_LEFT_23_GC_PERCENT=57.895
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_INTERNAL_23_GC_PERCENT=54.167
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=1.71
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=45.13
PRIMER_RIGHT_23_HAIRPIN_TH=32.30
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_23_END_STABILITY=2.9700
PRIMER_RIGHT_23_END_STABILITY=4.4600
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=3.1819
PRIMER_LEFT_24_PENALTY=1.926077
PRIMER_RIGHT_24_PENALTY=2.695830
PRIMER_INTERNAL_24_PENALTY=4.001118
PRIMER_LEFT_24_SEQUENCE=ATTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_24_SEQUENCE=GGATCGAATGGCCCACTAATA
PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG
PRIMER_LEFT_24=60,21
PRIMER_RIGHT_24=79,21
PRIMER_INTERNAL_24=35,24
PRIMER_LEFT_24_TM=59.926
PRIMER_RIGHT_24_TM=57.304
PRIMER_INTERNAL_24_TM=60.001
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_INTERNAL_24_GC_PERCENT=54.167
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=10.70
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=40.47
PRIMER_RIGHT_24_HAIRPIN_TH=0.00
PRIMER_INTERNAL_24_HAIRPIN_TH=0.00
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_24_END_STABILITY=3.8500
PRIMER_RIGHT_24_END_STABILITY=0.9800
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.5023
PRIMER_LEFT_25_PENALTY=1.960819
PRIMER_RIGHT_25_PENALTY=2.754380
PRIMER_INTERNAL_25_PENALTY=4.058186
PRIMER_LEFT_25_SEQUENCE=GATCCCGACCCAAATCGATAG
PRIMER_RIGHT_25_SEQUENCE=CGGGATCGAATGGCCCACTA
PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC
PRIMER_LEFT_25=75,21
PRIMER_RIGHT_25=81,20
PRIMER_INTERNAL_25=67,24
PRIMER_LEFT_25_TM=58.039
PRIMER_RIGHT_25_TM=61.754
PRIMER_INTERNAL_25_TM=59.942
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=60.000
PRIMER_INTERNAL_25_GC_PERCENT=58.333
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=45.13
PRIMER_RIGHT_25_HAIRPIN_TH=42.14
PRIMER_INTERNAL_25_HAIRPIN_TH=41.60
PRIMER_LEFT_25_LIBRARY_MISPRIMING=10.00, MSTa consensus sequence
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_25_END_STABILITY=2.0800
PRIMER_RIGHT_25_END_STABILITY=2.7400
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=9.7973
PRIMER_LEFT_26_PENALTY=1.997913
PRIMER_RIGHT_26_PENALTY=2.831715
PRIMER_INTERNAL_26_PENALTY=4.099823
PRIMER_LEFT_26_SEQUENCE=ATCCCGACCCAAATCGATAGC
PRIMER_RIGHT_26_SEQUENCE=TCGTCAGTGTCATGTCTACTG
PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_26=76,21
PRIMER_RIGHT_26=26,21
PRIMER_INTERNAL_26=66,23
PRIMER_LEFT_26_TM=59.998
PRIMER_RIGHT_26_TM=57.168
PRIMER_INTERNAL_26_TM=61.100
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=60.870
PRIMER_INTERNAL_26_SELF_ANY_TH=2.18
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=15.66
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=15.66
PRIMER_LEFT_26_HAIRPIN_TH=45.13
PRIMER_RIGHT_26_HAIRPIN_TH=42.34
PRIMER_INTERNAL_26_HAIRPIN_TH=41.60
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=2.9700
PRIMER_RIGHT_26_END_STABILITY=2.7400
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=24.9329
PRIMER_LEFT_27_PENALTY=2.038681
PRIMER_RIGHT_27_PENALTY=2.831715
PRIMER_INTERNAL_27_PENALTY=4.159384
PRIMER_LEFT_27_SEQUENCE=TCGATCCCGACCCAAATCGA
PRIMER_RIGHT_27_SEQUENCE=CGTCAGTGTCATGTCTACTGA
PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT
PRIMER_LEFT_27=73,20
PRIMER_RIGHT_27=25,21
PRIMER_INTERNAL_27=38,22
PRIMER_LEFT_27_TM=61.039
PRIMER_RIGHT_27_TM=57.168
PRIMER_INTERNAL_27_TM=57.841
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=54.545
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=2.47
PRIMER_RIGHT_27_SELF_ANY_TH=18.56
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=2.47
PRIMER_RIGHT_27_SELF_END_TH=18.56
PRIMER_LEFT_27_HAIRPIN_TH=45.41
PRIMER_RIGHT_27_HAIRPIN_TH=46.05
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER44C Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=3.5900
PRIMER_RIGHT_27_END_STABILITY=3.4100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=26.0802
PRIMER_LEFT_28_PENALTY=2.150848
PRIMER_RIGHT_28_PENALTY=2.912899
PRIMER_INTERNAL_28_PENALTY=4.159384
PRIMER_LEFT_28_SEQUENCE=TAGACATGACACTGACGATGC
PRIMER_RIGHT_28_SEQUENCE=TGCATCGTCAGTGTCATGT
PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG
PRIMER_LEFT_28=9,21
PRIMER_RIGHT_28=30,19
PRIMER_INTERNAL_28=37,22
PRIMER_LEFT_28_TM=57.849
PRIMER_RIGHT_28_TM=57.087
PRIMER_INTERNAL_28_TM=57.841
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.368
PRIMER_INTERNAL_28_GC_PERCENT=54.545
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=0.00
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_28_END_STABILITY=3.9100
PRIMER_RIGHT_28_END_STABILITY=3.2100
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=3.7339
PRIMER_LEFT_29_PENALTY=2.469564
PRIMER_RIGHT_29_PENALTY=2.989104
PRIMER_INTERNAL_29_PENALTY=4.196497
PRIMER_LEFT_29_SEQUENCE=CATTCGATCCCGACCCAAA
PRIMER_RIGHT_29_SEQUENCE=AGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG
PRIMER_LEFT_29=70,19
PRIMER_RIGHT_29=97,19
PRIMER_INTERNAL_29=36,23
PRIMER_LEFT_29_TM=57.530
PRIMER_RIGHT_29_TM=57.011
PRIMER_INTERNAL_29_TM=58.804
PRIMER_LEFT_29_GC_PERCENT=52.632
PRIMER_RIGHT_29_GC_PERCENT=52.632
PRIMER_INTERNAL_29_GC_PERCENT=56.522
PRIMER_INTERNAL_29_SELF_ANY_TH=0.00
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=1.71
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=45.50
PRIMER_RIGHT_29_HAIRPIN_TH=41.74
PRIMER_INTERNAL_29_HAIRPIN_TH=0.00
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_29_END_STABILITY=3.2800
PRIMER_RIGHT_29_END_STABILITY=4.7900
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=2.2809
PRIMER_LEFT_30_PENALTY=2.476833
PRIMER_RIGHT_30_PENALTY=3.032680
PRIMER_INTERNAL_30_PENALTY=4.232604
PRIMER_LEFT_30_SEQUENCE=CCATTCGATCCCGACCCAAAT
PRIMER_RIGHT_30_SEQUENCE=AGCTATCGATTTGGGTCGGGA
PRIMER_INTERNAL_30_SEQUENCE=GCCATTCGATCCCGACCCAAATCG
PRIMER_LEFT_30=69,21
PRIMER_RIGHT_30=97,21
PRIMER_INTERNAL_30=68,24
PRIMER_LEFT_30_TM=60.477
PRIMER_RIGHT_30_TM=61.033
PRIMER_INTERNAL_30_TM=60.233
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_INTERNAL_30_GC_PERCENT=58.333
PRIMER_INTERNAL_30_SELF_ANY_TH=0.00
PRIMER_LEFT_30_SELF_ANY_TH=0.00
PRIMER_RIGHT_30_SELF_ANY_TH=1.71
PRIMER_INTERNAL_30_SELF_END_TH=0.00
PRIMER_LEFT_30_SELF_END_TH=0.00
PRIMER_RIGHT_30_SELF_END_TH=0.00
PRIMER_LEFT_30_HAIRPIN_TH=45.50
PRIMER_RIGHT_30_HAIRPIN_TH=41.74
PRIMER_INTERNAL_30_HAIRPIN_TH=41.60
PRIMER_LEFT_30_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_30_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_30_END_STABILITY=2.3200
PRIMER_RIGHT_30_END_STABILITY=5.1400
PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=2.4428
PRIMER_LEFT_31_PENALTY=2.496898
PRIMER_RIGHT_31_PENALTY=4.101796
PRIMER_INTERNAL_31_PENALTY=4.351864
PRIMER_LEFT_31_SEQUENCE=TGACACTGACGATGCAGAC
PRIMER_RIGHT_31_SEQUENCE=TCGGGATCGAATGGCCCAC
PRIMER_INTERNAL_31_SEQUENCE=TTAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_31=15,19
PRIMER_RIGHT_31=82,19
PRIMER_INTERNAL_31=61,24
PRIMER_LEFT_31_TM=57.503
PRIMER_RIGHT_31_TM=62.102
PRIMER_INTERNAL_31_TM=60.352
PRIMER_LEFT_31_GC_PERCENT=52.632
PRIMER_RIGHT_31_GC_PERCENT=63.158
PRIMER_INTERNAL_31_GC_PERCENT=58.333
PRIMER_INTERNAL_31_SELF_ANY_TH=7.03
PRIMER_LEFT_31_SELF_ANY_TH=10.65
PRIMER_RIGHT_31_SELF_ANY_TH=0.00
PRIMER_INTERNAL_31_SELF_END_TH=0.00
PRIMER_LEFT_31_SELF_END_TH=0.00
PRIMER_RIGHT_31_SELF_END_TH=0.00
PRIMER_LEFT_31_HAIRPIN_TH=39.20
PRIMER_RIGHT_31_HAIRPIN_TH=43.03
PRIMER_INTERNAL_31_HAIRPIN_TH=41.60
PRIMER_LEFT_31_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_31_END_STABILITY=3.5100
PRIMER_RIGHT_31_END_STABILITY=4.6100
PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=16.1639
PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=11.0013
PRIMER_LEFT_32_PENALTY=2.540363
PRIMER_RIGHT_32_PENALTY=4.106986
PRIMER_INTERNAL_32_PENALTY=4.478673
PRIMER_LEFT_32_SEQUENCE=ATTCGATCCCGACCCAAATCG
PRIMER_RIGHT_32_SEQUENCE=CGGGATCGAATGGCCCACT
PRIMER_INTERNAL_32_SEQUENCE=TTAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_32=71,21
PRIMER_RIGHT_32=81,19
PRIMER_INTERNAL_32=61,23
PRIMER_LEFT_32_TM=60.540
PRIMER_RIGHT_32_TM=62.107
PRIMER_INTERNAL_32_TM=58.521
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=63.158
PRIMER_INTERNAL_32_GC_PERCENT=56.522
PRIMER_INTERNAL_32_SELF_ANY_TH=7.03
PRIMER_LEFT_32_SELF_ANY_TH=0.00
PRIMER_RIGHT_32_SELF_ANY_TH=0.00
PRIMER_INTERNAL_32_SELF_END_TH=0.00
PRIMER_LEFT_32_SELF_END_TH=0.00
PRIMER_RIGHT_32_SELF_END_TH=0.00
PRIMER_LEFT_32_HAIRPIN_TH=45.50
PRIMER_RIGHT_32_HAIRPIN_TH=42.14
PRIMER_INTERNAL_32_HAIRPIN_TH=41.60
PRIMER_LEFT_32_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_32_END_STABILITY=3.3400
PRIMER_RIGHT_32_END_STABILITY=4.0000
PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=2.4855
PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=10.5305
PRIMER_LEFT_33_PENALTY=2.637998
PRIMER_RIGHT_33_PENALTY=4.250296
PRIMER_INTERNAL_33_PENALTY=4.484274
PRIMER_LEFT_33_SEQUENCE=GACCCAAATCGATAGCTACGA
PRIMER_RIGHT_33_SEQUENCE=CGGGATCGAATGGCCCACTAA
PRIMER_INTERNAL_33_SEQUENCE=TTAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_33=81,21
PRIMER_RIGHT_33=81,21
PRIMER_INTERNAL_33=61,22
PRIMER_LEFT_33_TM=57.362
PRIMER_RIGHT_33_TM=62.250
PRIMER_INTERNAL_33_TM=57.516
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=57.143
PRIMER_INTERNAL_33_GC_PERCENT=54.545
PRIMER_INTERNAL_33_SELF_ANY_TH=7.03
PRIMER_LEFT_33_SELF_ANY_TH=16.85
PRIMER_RIGHT_33_SELF_ANY_TH=0.00
PRIMER_INTERNAL_33_SELF_END_TH=0.00
PRIMER_LEFT_33_SELF_END_TH=16.85
PRIMER_RIGHT_33_SELF_END_TH=0.00
PRIMER_LEFT_33_HAIRPIN_TH=43.65
PRIMER_RIGHT_33_HAIRPIN_TH=42.14
PRIMER_INTERNAL_33_HAIRPIN_TH=39.49
PRIMER_LEFT_33_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_RIGHT_33_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_33_END_STABILITY=3.4300
PRIMER_RIGHT_33_END_STABILITY=2.2400
PRIMER_LEFT_33_TEMPLATE_MISPRIMING_TH=20.0470
PRIMER_RIGHT_33_TEMPLATE_MISPRIMING_TH=10.9108
PRIMER_LEFT_34_PENALTY=2.695830
PRIMER_INTERNAL_34_PENALTY=4.552303
PRIMER_LEFT_34_SEQUENCE=TATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_34_SEQUENCE=ACACACACACACACAGCACACA
PRIMER_LEFT_34=59,21
PRIMER_INTERNAL_34=35,22
PRIMER_LEFT_34_TM=57.304
PRIMER_INTERNAL_34_TM=57.448
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_INTERNAL_34_GC_PERCENT=50.000
PRIMER_INTERNAL_34_SELF_ANY_TH=0.00
PRIMER_LEFT_34_SELF_ANY_TH=10.70
PRIMER_INTERNAL_34_SELF_END_TH=0.00
PRIMER_LEFT_34_SELF_END_TH=0.00
PRIMER_LEFT_34_HAIRPIN_TH=0.00
PRIMER_INTERNAL_34_HAIRPIN_TH=0.00
PRIMER_LEFT_34_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_34_END_STABILITY=3.3600
PRIMER_LEFT_34_TEMPLATE_MISPRIMING_TH=9.4710
PRIMER_LEFT_35_PENALTY=2.754380
PRIMER_INTERNAL_35_PENALTY=4.639434
PRIMER_LEFT_35_SEQUENCE=TAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_35_SEQUENCE=GCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_35=62,20
PRIMER_INTERNAL_35=68,22
PRIMER_LEFT_35_TM=61.754
PRIMER_INTERNAL_35_TM=57.361
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_INTERNAL_35_GC_PERCENT=54.545
PRIMER_INTERNAL_35_SELF_ANY_TH=0.00
PRIMER_LEFT_35_SELF_ANY_TH=10.70
PRIMER_INTERNAL_35_SELF_END_TH=0.00
PRIMER_LEFT_35_SELF_END_TH=0.00
PRIMER_LEFT_35_HAIRPIN_TH=42.98
PRIMER_INTERNAL_35_HAIRPIN_TH=41.60
PRIMER_LEFT_35_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_35_END_STABILITY=5.1400
PRIMER_LEFT_35_TEMPLATE_MISPRIMING_TH=6.4478
PRIMER_LEFT_36_PENALTY=2.772619
PRIMER_INTERNAL_36_PENALTY=4.809017
PRIMER_LEFT_36_SEQUENCE=TTCGATCCCGACCCAAATC
PRIMER_INTERNAL_36_SEQUENCE=ACACACACACACACAGCACACAG
PRIMER_LEFT_36=72,19
PRIMER_INTERNAL_36=35,23
PRIMER_LEFT_36_TM=57.227
PRIMER_INTERNAL_36_TM=58.191
PRIMER_LEFT_36_GC_PERCENT=52.632
PRIMER_INTERNAL_36_GC_PERCENT=52.174
PRIMER_INTERNAL_36_SELF_ANY_TH=0.00
PRIMER_LEFT_36_SELF_ANY_TH=0.00
PRIMER_INTERNAL_36_SELF_END_TH=0.00
PRIMER_LEFT_36_SELF_END_TH=0.00
PRIMER_LEFT_36_HAIRPIN_TH=45.50
PRIMER_INTERNAL_36_HAIRPIN_TH=0.00
PRIMER_LEFT_36_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_36_END_STABILITY=2.1700
PRIMER_LEFT_36_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_37_PENALTY=2.831715
PRIMER_INTERNAL_37_PENALTY=4.881095
PRIMER_LEFT_37_SEQUENCE=CAGTAGACATGACACTGACGA
PRIMER_INTERNAL_37_SEQUENCE=GCCATTCGATCCCGACCCAAATC
PRIMER_LEFT_37=6,21
PRIMER_INTERNAL_37=68,23
PRIMER_LEFT_37_TM=57.168
PRIMER_INTERNAL_37_TM=58.119
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_INTERNAL_37_GC_PERCENT=56.522
PRIMER_INTERNAL_37_SELF_ANY_TH=0.00
PRIMER_LEFT_37_SELF_ANY_TH=16.10
PRIMER_INTERNAL_37_SELF_END_TH=0.00
PRIMER_LEFT_37_SELF_END_TH=0.00
PRIMER_LEFT_37_HAIRPIN_TH=41.80
PRIMER_INTERNAL_37_HAIRPIN_TH=41.60
PRIMER_LEFT_37_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_37_END_STABILITY=4.2000
PRIMER_LEFT_37_TEMPLATE_MISPRIMING_TH=20.3033
PRIMER_LEFT_38_PENALTY=2.831715
PRIMER_INTERNAL_38_PENALTY=5.108025
PRIMER_LEFT_38_SEQUENCE=TCAGTAGACATGACACTGACG
PRIMER_INTERNAL_38_SEQUENCE=TGGGCCATTCGATCCCGACCCAA
PRIMER_LEFT_38=5,21
PRIMER_INTERNAL_38=65,23
PRIMER_LEFT_38_TM=57.168
PRIMER_INTERNAL_38_TM=62.108
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_INTERNAL_38_GC_PERCENT=60.870
PRIMER_INTERNAL_38_SELF_ANY_TH=0.00
PRIMER_LEFT_38_SELF_ANY_TH=18.35
PRIMER_INTERNAL_38_SELF_END_TH=0.00
PRIMER_LEFT_38_SELF_END_TH=0.00
PRIMER_LEFT_38_HAIRPIN_TH=42.75
PRIMER_INTERNAL_38_HAIRPIN_TH=41.29
PRIMER_LEFT_38_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_38_END_STABILITY=4.3500
PRIMER_LEFT_38_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_39_PENALTY=2.912899
PRIMER_INTERNAL_39_PENALTY=5.111368
PRIMER_LEFT_39_SEQUENCE=ACATGACACTGACGATGCA
PRIMER_INTERNAL_39_SEQUENCE=CCATTCGATCCCGACCCAAATCGA
PRIMER_LEFT_39=12,19
PRIMER_INTERNAL_39=69,24
PRIMER_LEFT_39_TM=57.087
PRIMER_INTERNAL_39_TM=58.889
PRIMER_LEFT_39_GC_PERCENT=47.368
PRIMER_INTERNAL_39_GC_PERCENT=54.167
PRIMER_INTERNAL_39_SELF_ANY_TH=0.00
PRIMER_LEFT_39_SELF_ANY_TH=0.00
PRIMER_INTERNAL_39_SELF_END_TH=0.00
PRIMER_LEFT_39_SELF_END_TH=0.00
PRIMER_LEFT_39_HAIRPIN_TH=0.00
PRIMER_INTERNAL_39_HAIRPIN_TH=42.01
PRIMER_LEFT_39_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_39_END_STABILITY=3.9600
PRIMER_LEFT_39_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_40_PENALTY=2.989104
PRIMER_INTERNAL_40_PENALTY=5.111455
PRIMER_LEFT_40_SEQUENCE=CCGACCCAAATCGATAGCT
PRIMER_INTERNAL_40_SEQUENCE=CCATTCGATCCCGACCCAAATCG
PRIMER_LEFT_40=79,19
PRIMER_INTERNAL_40=69,23
PRIMER_LEFT_40_TM=57.011
PRIMER_INTERNAL_40_TM=57.889
PRIMER_LEFT_40_GC_PERCENT=52.632
PRIMER_INTERNAL_40_GC_PERCENT=56.522
PRIMER_INTERNAL_40_SELF_ANY_TH=0.00
PRIMER_LEFT_40_SELF_ANY_TH=0.00
PRIMER_INTERNAL_40_SELF_END_TH=0.00
PRIMER_LEFT_40_SELF_END_TH=0.00
PRIMER_LEFT_40_HAIRPIN_TH=45.13
PRIMER_INTERNAL_40_HAIRPIN_TH=41.60
PRIMER_LEFT_40_LIBRARY_MISPRIMING=9.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_LEFT_40_END_STABILITY=3.3200
PRIMER_LEFT_40_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_LEFT_41_PENALTY=3.032680
PRIMER_INTERNAL_41_PENALTY=5.122599
PRIMER_LEFT_41_SEQUENCE=TCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_41_SEQUENCE=GGGCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_41=77,21
PRIMER_INTERNAL_41=66,24
PRIMER_LEFT_41_TM=61.033
PRIMER_INTERNAL_41_TM=61.123
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_INTERNAL_41_GC_PERCENT=58.333
PRIMER_INTERNAL_41_SELF_ANY_TH=2.18
PRIMER_LEFT_41_SELF_ANY_TH=0.00
PRIMER_INTERNAL_41_SELF_END_TH=0.00
PRIMER_LEFT_41_SELF_END_TH=0.00
PRIMER_LEFT_41_HAIRPIN_TH=45.13
PRIMER_INTERNAL_41_HAIRPIN_TH=41.60
PRIMER_LEFT_41_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_41_END_STABILITY=3.3200
PRIMER_LEFT_41_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_LEFT_42_PENALTY=3.367844
PRIMER_INTERNAL_42_PENALTY=5.295331
PRIMER_LEFT_42_SEQUENCE=TCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_42_SEQUENCE=ACACACACACACAGCACACAGGTA
PRIMER_LEFT_42=73,21
PRIMER_INTERNAL_42=37,24
PRIMER_LEFT_42_TM=61.368
PRIMER_INTERNAL_42_TM=58.705
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_INTERNAL_42_GC_PERCENT=50.000
PRIMER_INTERNAL_42_SELF_ANY_TH=0.00
PRIMER_LEFT_42_SELF_ANY_TH=0.00
PRIMER_INTERNAL_42_SELF_END_TH=0.00
PRIMER_LEFT_42_SELF_END_TH=0.00
PRIMER_LEFT_42_HAIRPIN_TH=43.48
PRIMER_INTERNAL_42_HAIRPIN_TH=0.00
PRIMER_LEFT_42_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_42_END_STABILITY=3.5900
PRIMER_LEFT_42_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_43_PENALTY=3.568322
PRIMER_INTERNAL_43_PENALTY=5.316271
PRIMER_LEFT_43_SEQUENCE=TTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_43_SEQUENCE=ATTAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_43=72,21
PRIMER_INTERNAL_43=60,23
PRIMER_LEFT_43_TM=61.568
PRIMER_INTERNAL_43_TM=57.684
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_INTERNAL_43_GC_PERCENT=52.174
PRIMER_INTERNAL_43_SELF_ANY_TH=7.03
PRIMER_LEFT_43_SELF_ANY_TH=2.47
PRIMER_INTERNAL_43_SELF_END_TH=0.00
PRIMER_LEFT_43_SELF_END_TH=2.47
PRIMER_LEFT_43_HAIRPIN_TH=45.78
PRIMER_INTERNAL_43_HAIRPIN_TH=39.49
PRIMER_LEFT_43_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_43_END_STABILITY=3.5900
PRIMER_LEFT_43_TEMPLATE_MISPRIMING_TH=4.8294
PRIMER_LEFT_44_PENALTY=3.602746
PRIMER_INTERNAL_44_PENALTY=5.357946
PRIMER_LEFT_44_SEQUENCE=GCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_44_SEQUENCE=ATTAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_44=68,20
PRIMER_INTERNAL_44=60,24
PRIMER_LEFT_44_TM=62.603
PRIMER_INTERNAL_44_TM=58.642
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_INTERNAL_44_GC_PERCENT=54.167
PRIMER_INTERNAL_44_SELF_ANY_TH=7.03
PRIMER_LEFT_44_SELF_ANY_TH=0.00
PRIMER_INTERNAL_44_SELF_END_TH=0.00
PRIMER_LEFT_44_SELF_END_TH=0.00
PRIMER_LEFT_44_HAIRPIN_TH=45.50
PRIMER_INTERNAL_44_HAIRPIN_TH=41.60
PRIMER_LEFT_44_LIBRARY_MISPRIMING=10.00, MER4A a consensus
PRIMER_LEFT_44_END_STABILITY=4.1200
PRIMER_LEFT_44_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_45_PENALTY=4.101796
PRIMER_INTERNAL_45_PENALTY=5.401146
PRIMER_LEFT_45_SEQUENCE=GCCATTCGATCCCGACCCA
PRIMER_INTERNAL_45_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_45=68,19
PRIMER_INTERNAL_45=60,25
PRIMER_LEFT_45_TM=62.102
PRIMER_INTERNAL_45_TM=60.401
PRIMER_LEFT_45_GC_PERCENT=63.158
PRIMER_INTERNAL_45_GC_PERCENT=56.000
PRIMER_INTERNAL_45_SELF_ANY_TH=7.03
PRIMER_LEFT_45_SELF_ANY_TH=0.00
PRIMER_INTERNAL_45_SELF_END_TH=0.00
PRIMER_LEFT_45_SELF_END_TH=0.00
PRIMER_LEFT_45_HAIRPIN_TH=45.50
PRIMER_INTERNAL_45_HAIRPIN_TH=41.60
PRIMER_LEFT_45_LIBRARY_MISPRIMING=10.00, TCCCA Primate simple repeat pattern
PRIMER_LEFT_45_END_STABILITY=4.5100
PRIMER_LEFT_45_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_46_PENALTY=4.101796
PRIMER_INTERNAL_46_PENALTY=5.439697
PRIMER_LEFT_46_SEQUENCE=TGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_46_SEQUENCE=CACACACACACACAGCACACAGGTA
PRIMER_LEFT_46=65,19
PRIMER_INTERNAL_46=36,25
PRIMER_LEFT_46_TM=62.102
PRIMER_INTERNAL_46_TM=59.560
PRIMER_LEFT_46_GC_PERCENT=63.158
PRIMER_INTERNAL_46_GC_PERCENT=52.000
PRIMER_INTERNAL_46_SELF_ANY_TH=0.00
PRIMER_LEFT_46_SELF_ANY_TH=0.00
PRIMER_INTERNAL_46_SELF_END_TH=0.00
PRIMER_LEFT_46_SELF_END_TH=0.00
PRIMER_LEFT_46_HAIRPIN_TH=45.50
PRIMER_INTERNAL_46_HAIRPIN_TH=0.00
PRIMER_LEFT_46_LIBRARY_MISPRIMING=9.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_46_END_STABILITY=4.7900
PRIMER_LEFT_46_TEMPLATE_MISPRIMING_TH=4.9124
PRIMER_LEFT_47_PENALTY=4.101796
PRIMER_INTERNAL_47_PENALTY=5.459011
PRIMER_LEFT_47_SEQUENCE=GTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_47_SEQUENCE=AGTGGGCCATTCGATCCCGACCC
PRIMER_LEFT_47=64,19
PRIMER_INTERNAL_47=63,23
PRIMER_LEFT_47_TM=62.102
PRIMER_INTERNAL_47_TM=62.459
PRIMER_LEFT_47_GC_PERCENT=63.158
PRIMER_INTERNAL_47_GC_PERCENT=65.217
PRIMER_INTERNAL_47_SELF_ANY_TH=7.03
PRIMER_LEFT_47_SELF_ANY_TH=10.70
PRIMER_INTERNAL_47_SELF_END_TH=0.00
PRIMER_LEFT_47_SELF_END_TH=0.00
PRIMER_LEFT_47_HAIRPIN_TH=42.98
PRIMER_INTERNAL_47_HAIRPIN_TH=41.60
PRIMER_LEFT_47_LIBRARY_MISPRIMING=9.00, MER1B Nonautonomous DNA transposon
PRIMER_LEFT_47_END_STABILITY=5.1400
PRIMER_LEFT_47_TEMPLATE_MISPRIMING_TH=10.7677
PRIMER_LEFT_48_PENALTY=4.106986
PRIMER_INTERNAL_48_PENALTY=5.514983
PRIMER_LEFT_48_SEQUENCE=AGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_48_SEQUENCE=TCGATCCCGACCCAAATCGATAGC
PRIMER_LEFT_48=63,19
PRIMER_INTERNAL_48=73,24
PRIMER_LEFT_48_TM=62.107
PRIMER_INTERNAL_48_TM=58.485
PRIMER_LEFT_48_GC_PERCENT=63.158
PRIMER_INTERNAL_48_GC_PERCENT=54.167
PRIMER_INTERNAL_48_SELF_ANY_TH=0.00
PRIMER_LEFT_48_SELF_ANY_TH=10.70
PRIMER_INTERNAL_48_SELF_END_TH=0.00
PRIMER_LEFT_48_SELF_END_TH=0.00
PRIMER_LEFT_48_HAIRPIN_TH=42.98
PRIMER_INTERNAL_48_HAIRPIN_TH=38.51
PRIMER_LEFT_48_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_48_END_STABILITY=5.1400
PRIMER_LEFT_48_TEMPLATE_MISPRIMING_TH=6.4478
PRIMER_LEFT_49_PENALTY=4.250296
PRIMER_INTERNAL_49_PENALTY=5.518553
PRIMER_LEFT_49_SEQUENCE=TTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_49_SEQUENCE=CGATCCCGACCCAAATCGATAGCT
PRIMER_LEFT_49=61,21
PRIMER_INTERNAL_49=74,24
PRIMER_LEFT_49_TM=62.250
PRIMER_INTERNAL_49_TM=58.481
PRIMER_LEFT_49_GC_PERCENT=57.143
PRIMER_INTERNAL_49_GC_PERCENT=54.167
PRIMER_INTERNAL_49_SELF_ANY_TH=0.00
PRIMER_LEFT_49_SELF_ANY_TH=10.70
PRIMER_INTERNAL_49_SELF_END_TH=0.00
PRIMER_LEFT_49_SELF_END_TH=0.00
PRIMER_LEFT_49_HAIRPIN_TH=42.98
PRIMER_INTERNAL_49_HAIRPIN_TH=41.28
PRIMER_LEFT_49_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_49_END_STABILITY=5.1400
PRIMER_LEFT_49_TEMPLATE_MISPRIMING_TH=6.4478
PRIMER_LEFT_50_PENALTY=4.826926
PRIMER_INTERNAL_50_PENALTY=5.523565
PRIMER_LEFT_50_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_INTERNAL_50_SEQUENCE=CGATCCCGACCCAAATCGATAGC
PRIMER_LEFT_50=67,19
PRIMER_INTERNAL_50=74,23
PRIMER_LEFT_50_TM=62.827
PRIMER_INTERNAL_50_TM=57.476
PRIMER_LEFT_50_GC_PERCENT=68.421
PRIMER_INTERNAL_50_GC_PERCENT=56.522
PRIMER_INTERNAL_50_SELF_ANY_TH=0.00
PRIMER_LEFT_50_SELF_ANY_TH=0.00
PRIMER_INTERNAL_50_SELF_END_TH=0.00
PRIMER_LEFT_50_SELF_END_TH=0.00
PRIMER_LEFT_50_HAIRPIN_TH=45.50
PRIMER_INTERNAL_50_HAIRPIN_TH=41.28
PRIMER_LEFT_50_LIBRARY_MISPRIMING=9.00, TCCCA Primate simple repeat pattern
PRIMER_LEFT_50_END_STABILITY=4.4600
PRIMER_LEFT_50_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_51_PENALTY=4.826926
PRIMER_INTERNAL_51_PENALTY=5.550131
PRIMER_LEFT_51_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_INTERNAL_51_SEQUENCE=CACACACACACAGCACACAGGTA
PRIMER_LEFT_51=66,19
PRIMER_INTERNAL_51=38,23
PRIMER_LEFT_51_TM=62.827
PRIMER_INTERNAL_51_TM=57.450
PRIMER_LEFT_51_GC_PERCENT=68.421
PRIMER_INTERNAL_51_GC_PERCENT=52.174
PRIMER_INTERNAL_51_SELF_ANY_TH=0.00
PRIMER_LEFT_51_SELF_ANY_TH=4.39
PRIMER_INTERNAL_51_SELF_END_TH=0.00
PRIMER_LEFT_51_SELF_END_TH=0.00
PRIMER_LEFT_51_HAIRPIN_TH=45.50
PRIMER_INTERNAL_51_HAIRPIN_TH=0.00
PRIMER_LEFT_51_LIBRARY_MISPRIMING=9.00, HSATII Human satellite II DNA
PRIMER_LEFT_51_END_STABILITY=4.7900
PRIMER_LEFT_51_TEMPLATE_MISPRIMING_TH=3.1645
PRIMER_INTERNAL_52_PENALTY=5.564155
PRIMER_INTERNAL_52_SEQUENCE=TCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_52=73,25
PRIMER_INTERNAL_52_TM=59.436
PRIMER_INTERNAL_52_GC_PERCENT=52.000
PRIMER_INTERNAL_52_SELF_ANY_TH=0.00
PRIMER_INTERNAL_52_SELF_END_TH=0.00
PRIMER_INTERNAL_52_HAIRPIN_TH=38.51
PRIMER_INTERNAL_53_PENALTY=5.697915
PRIMER_INTERNAL_53_SEQUENCE=GTGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_53=64,23
PRIMER_INTERNAL_53_TM=62.698
PRIMER_INTERNAL_53_GC_PERCENT=65.217
PRIMER_INTERNAL_53_SELF_ANY_TH=7.03
PRIMER_INTERNAL_53_SELF_END_TH=3.99
PRIMER_INTERNAL_53_HAIRPIN_TH=42.67
PRIMER_INTERNAL_54_PENALTY=5.806699
PRIMER_INTERNAL_54_SEQUENCE=CCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_54=78,24
PRIMER_INTERNAL_54_TM=58.193
PRIMER_INTERNAL_54_GC_PERCENT=54.167
PRIMER_INTERNAL_54_SELF_ANY_TH=12.68
PRIMER_INTERNAL_54_SELF_END_TH=12.68
PRIMER_INTERNAL_54_HAIRPIN_TH=41.28
PRIMER_INTERNAL_55_PENALTY=5.806699
PRIMER_INTERNAL_55_SEQUENCE=TCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_55=77,24
PRIMER_INTERNAL_55_TM=58.193
PRIMER_INTERNAL_55_GC_PERCENT=54.167
PRIMER_INTERNAL_55_SELF_ANY_TH=0.00
PRIMER_INTERNAL_55_SELF_END_TH=0.00
PRIMER_INTERNAL_55_HAIRPIN_TH=41.28
PRIMER_INTERNAL_56_PENALTY=5.824076
PRIMER_INTERNAL_56_SEQUENCE=CCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_56=78,23
PRIMER_INTERNAL_56_TM=57.176
PRIMER_INTERNAL_56_GC_PERCENT=56.522
PRIMER_INTERNAL_56_SELF_ANY_TH=0.00
PRIMER_INTERNAL_56_SELF_END_TH=0.00
PRIMER_INTERNAL_56_HAIRPIN_TH=41.28
PRIMER_INTERNAL_57_PENALTY=5.846052
PRIMER_INTERNAL_57_SEQUENCE=TCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_57=77,25
PRIMER_INTERNAL_57_TM=59.154
PRIMER_INTERNAL_57_GC_PERCENT=52.000
PRIMER_INTERNAL_57_SELF_ANY_TH=12.68
PRIMER_INTERNAL_57_SELF_END_TH=12.68
PRIMER_INTERNAL_57_HAIRPIN_TH=41.28
PRIMER_INTERNAL_58_PENALTY=5.892980
PRIMER_INTERNAL_58_SEQUENCE=TAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_58=62,24
PRIMER_INTERNAL_58_TM=61.893
PRIMER_INTERNAL_58_GC_PERCENT=62.500
PRIMER_INTERNAL_58_SELF_ANY_TH=7.03
PRIMER_INTERNAL_58_SELF_END_TH=0.00
PRIMER_INTERNAL_58_HAIRPIN_TH=41.60
PRIMER_INTERNAL_59_PENALTY=5.951461
PRIMER_INTERNAL_59_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_59=57,25
PRIMER_INTERNAL_59_TM=59.049
PRIMER_INTERNAL_59_GC_PERCENT=56.000
PRIMER_INTERNAL_59_SELF_ANY_TH=7.03
PRIMER_INTERNAL_59_SELF_END_TH=0.00
PRIMER_INTERNAL_59_HAIRPIN_TH=39.49
PRIMER_INTERNAL_60_PENALTY=5.980536
PRIMER_INTERNAL_60_SEQUENCE=CATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_60=70,23
PRIMER_INTERNAL_60_TM=57.019
PRIMER_INTERNAL_60_GC_PERCENT=52.174
PRIMER_INTERNAL_60_SELF_ANY_TH=0.00
PRIMER_INTERNAL_60_SELF_END_TH=0.00
PRIMER_INTERNAL_60_HAIRPIN_TH=42.01
PRIMER_INTERNAL_61_PENALTY=6.009338
PRIMER_INTERNAL_61_SEQUENCE=CCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_61=69,25
PRIMER_INTERNAL_61_TM=58.991
PRIMER_INTERNAL_61_GC_PERCENT=52.000
PRIMER_INTERNAL_61_SELF_ANY_TH=2.97
PRIMER_INTERNAL_61_SELF_END_TH=0.00
PRIMER_INTERNAL_61_HAIRPIN_TH=41.68
PRIMER_INTERNAL_62_PENALTY=6.046206
PRIMER_INTERNAL_62_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_62=59,26
PRIMER_INTERNAL_62_TM=59.954
PRIMER_INTERNAL_62_GC_PERCENT=53.846
PRIMER_INTERNAL_62_SELF_ANY_TH=7.03
PRIMER_INTERNAL_62_SELF_END_TH=0.00
PRIMER_INTERNAL_62_HAIRPIN_TH=41.60
PRIMER_INTERNAL_63_PENALTY=6.046206
PRIMER_INTERNAL_63_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_63=57,26
PRIMER_INTERNAL_63_TM=59.954
PRIMER_INTERNAL_63_GC_PERCENT=53.846
PRIMER_INTERNAL_63_SELF_ANY_TH=7.03
PRIMER_INTERNAL_63_SELF_END_TH=0.00
PRIMER_INTERNAL_63_HAIRPIN_TH=39.49
PRIMER_INTERNAL_64_PENALTY=6.046659
PRIMER_INTERNAL_64_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_64=56,26
PRIMER_INTERNAL_64_TM=59.953
PRIMER_INTERNAL_64_GC_PERCENT=53.846
PRIMER_INTERNAL_64_SELF_ANY_TH=7.03
PRIMER_INTERNAL_64_SELF_END_TH=0.00
PRIMER_INTERNAL_64_HAIRPIN_TH=39.49
PRIMER_INTERNAL_65_PENALTY=6.125318
PRIMER_INTERNAL_65_SEQUENCE=GCCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_65=68,25
PRIMER_INTERNAL_65_TM=61.125
PRIMER_INTERNAL_65_GC_PERCENT=56.000
PRIMER_INTERNAL_65_SELF_ANY_TH=0.00
PRIMER_INTERNAL_65_SELF_END_TH=0.00
PRIMER_INTERNAL_65_HAIRPIN_TH=42.01
PRIMER_INTERNAL_66_PENALTY=6.131527
PRIMER_INTERNAL_66_SEQUENCE=ACACACACACACACAGCACACAGGT
PRIMER_INTERNAL_66=35,25
PRIMER_INTERNAL_66_TM=61.132
PRIMER_INTERNAL_66_GC_PERCENT=52.000
PRIMER_INTERNAL_66_SELF_ANY_TH=0.00
PRIMER_INTERNAL_66_SELF_END_TH=0.00
PRIMER_INTERNAL_66_HAIRPIN_TH=0.00
PRIMER_INTERNAL_67_PENALTY=6.186005
PRIMER_INTERNAL_67_SEQUENCE=ACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_67=37,25
PRIMER_INTERNAL_67_TM=58.814
PRIMER_INTERNAL_67_GC_PERCENT=48.000
PRIMER_INTERNAL_67_SELF_ANY_TH=0.00
PRIMER_INTERNAL_67_SELF_END_TH=0.00
PRIMER_INTERNAL_67_HAIRPIN_TH=0.00
PRIMER_INTERNAL_68_PENALTY=6.225896
PRIMER_INTERNAL_68_SEQUENCE=TTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_68=72,25
PRIMER_INTERNAL_68_TM=58.774
PRIMER_INTERNAL_68_GC_PERCENT=52.000
PRIMER_INTERNAL_68_SELF_ANY_TH=0.00
PRIMER_INTERNAL_68_SELF_END_TH=0.00
PRIMER_INTERNAL_68_HAIRPIN_TH=41.68
PRIMER_INTERNAL_69_PENALTY=6.265114
PRIMER_INTERNAL_69_SEQUENCE=TGGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_69=65,24
PRIMER_INTERNAL_69_TM=62.265
PRIMER_INTERNAL_69_GC_PERCENT=58.333
PRIMER_INTERNAL_69_SELF_ANY_TH=0.00
PRIMER_INTERNAL_69_SELF_END_TH=0.00
PRIMER_INTERNAL_69_HAIRPIN_TH=41.29
PRIMER_INTERNAL_70_PENALTY=6.318558
PRIMER_INTERNAL_70_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_70=72,26
PRIMER_INTERNAL_70_TM=59.681
PRIMER_INTERNAL_70_GC_PERCENT=50.000
PRIMER_INTERNAL_70_SELF_ANY_TH=0.00
PRIMER_INTERNAL_70_SELF_END_TH=0.00
PRIMER_INTERNAL_70_HAIRPIN_TH=41.68
PRIMER_INTERNAL_71_PENALTY=6.365515
PRIMER_INTERNAL_71_SEQUENCE=CACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_71=36,26
PRIMER_INTERNAL_71_TM=59.634
PRIMER_INTERNAL_71_GC_PERCENT=50.000
PRIMER_INTERNAL_71_SELF_ANY_TH=0.00
PRIMER_INTERNAL_71_SELF_END_TH=0.00
PRIMER_INTERNAL_71_HAIRPIN_TH=0.00
PRIMER_INTERNAL_72_PENALTY=6.390437
PRIMER_INTERNAL_72_SEQUENCE=CACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_72=38,24
PRIMER_INTERNAL_72_TM=57.610
PRIMER_INTERNAL_72_GC_PERCENT=50.000
PRIMER_INTERNAL_72_SELF_ANY_TH=0.00
PRIMER_INTERNAL_72_SELF_END_TH=0.00
PRIMER_INTERNAL_72_HAIRPIN_TH=0.00
PRIMER_INTERNAL_73_PENALTY=6.635581
PRIMER_INTERNAL_73_SEQUENCE=GGGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_73=66,25
PRIMER_INTERNAL_73_TM=61.636
PRIMER_INTERNAL_73_GC_PERCENT=60.000
PRIMER_INTERNAL_73_SELF_ANY_TH=2.18
PRIMER_INTERNAL_73_SELF_END_TH=0.00
PRIMER_INTERNAL_73_HAIRPIN_TH=41.60
PRIMER_INTERNAL_74_PENALTY=6.661393
PRIMER_INTERNAL_74_SEQUENCE=ACACACACACACACAGCACACAGGTA
PRIMER_INTERNAL_74=35,26
PRIMER_INTERNAL_74_TM=60.661
PRIMER_INTERNAL_74_GC_PERCENT=50.000
PRIMER_INTERNAL_74_SELF_ANY_TH=0.00
PRIMER_INTERNAL_74_SELF_END_TH=0.00
PRIMER_INTERNAL_74_HAIRPIN_TH=0.00
PRIMER_INTERNAL_75_PENALTY=6.679783
PRIMER_INTERNAL_75_SEQUENCE=CCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_75=78,25
PRIMER_INTERNAL_75_TM=58.320
PRIMER_INTERNAL_75_GC_PERCENT=52.000
PRIMER_INTERNAL_75_SELF_ANY_TH=14.48
PRIMER_INTERNAL_75_SELF_END_TH=14.48
PRIMER_INTERNAL_75_HAIRPIN_TH=46.54
PRIMER_INTERNAL_76_PENALTY=6.679783
PRIMER_INTERNAL_76_SEQUENCE=ATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_76=76,25
PRIMER_INTERNAL_76_TM=58.320
PRIMER_INTERNAL_76_GC_PERCENT=52.000
PRIMER_INTERNAL_76_SELF_ANY_TH=0.00
PRIMER_INTERNAL_76_SELF_END_TH=0.00
PRIMER_INTERNAL_76_HAIRPIN_TH=41.28
PRIMER_INTERNAL_77_PENALTY=6.695664
PRIMER_INTERNAL_77_SEQUENCE=TATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_77=59,24
PRIMER_INTERNAL_77_TM=57.304
PRIMER_INTERNAL_77_GC_PERCENT=50.000
PRIMER_INTERNAL_77_SELF_ANY_TH=7.03
PRIMER_INTERNAL_77_SELF_END_TH=0.00
PRIMER_INTERNAL_77_HAIRPIN_TH=39.49
PRIMER_INTERNAL_78_PENALTY=6.741797
PRIMER_INTERNAL_78_SEQUENCE=GTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_78=58,24
PRIMER_INTERNAL_78_TM=57.258
PRIMER_INTERNAL_78_GC_PERCENT=54.167
PRIMER_INTERNAL_78_SELF_ANY_TH=7.03
PRIMER_INTERNAL_78_SELF_END_TH=0.00
PRIMER_INTERNAL_78_HAIRPIN_TH=39.49
PRIMER_INTERNAL_79_PENALTY=6.754240
PRIMER_INTERNAL_79_SEQUENCE=TATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_79=59,25
PRIMER_INTERNAL_79_TM=58.246
PRIMER_INTERNAL_79_GC_PERCENT=52.000
PRIMER_INTERNAL_79_SELF_ANY_TH=7.03
PRIMER_INTERNAL_79_SELF_END_TH=0.00
PRIMER_INTERNAL_79_HAIRPIN_TH=41.60
PRIMER_INTERNAL_80_PENALTY=6.754240
PRIMER_INTERNAL_80_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_80=58,25
PRIMER_INTERNAL_80_TM=58.246
PRIMER_INTERNAL_80_GC_PERCENT=52.000
PRIMER_INTERNAL_80_SELF_ANY_TH=7.03
PRIMER_INTERNAL_80_SELF_END_TH=0.00
PRIMER_INTERNAL_80_HAIRPIN_TH=39.49
PRIMER_INTERNAL_81_PENALTY=6.756422
PRIMER_INTERNAL_81_SEQUENCE=TCCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_81=77,26
PRIMER_INTERNAL_81_TM=59.244
PRIMER_INTERNAL_81_GC_PERCENT=50.000
PRIMER_INTERNAL_81_SELF_ANY_TH=14.48
PRIMER_INTERNAL_81_SELF_END_TH=14.48
PRIMER_INTERNAL_81_HAIRPIN_TH=46.54
PRIMER_INTERNAL_82_PENALTY=6.756422
PRIMER_INTERNAL_82_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_82=76,26
PRIMER_INTERNAL_82_TM=59.244
PRIMER_INTERNAL_82_GC_PERCENT=50.000
PRIMER_INTERNAL_82_SELF_ANY_TH=12.68
PRIMER_INTERNAL_82_SELF_END_TH=12.68
PRIMER_INTERNAL_82_HAIRPIN_TH=41.28
PRIMER_INTERNAL_83_PENALTY=6.804314
PRIMER_INTERNAL_83_SEQUENCE=CATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_83=70,24
PRIMER_INTERNAL_83_TM=57.196
PRIMER_INTERNAL_83_GC_PERCENT=50.000
PRIMER_INTERNAL_83_SELF_ANY_TH=2.97
PRIMER_INTERNAL_83_SELF_END_TH=0.00
PRIMER_INTERNAL_83_HAIRPIN_TH=41.68
PRIMER_INTERNAL_84_PENALTY=6.828710
PRIMER_INTERNAL_84_SEQUENCE=GTGGGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_84=64,24
PRIMER_INTERNAL_84_TM=62.829
PRIMER_INTERNAL_84_GC_PERCENT=62.500
PRIMER_INTERNAL_84_SELF_ANY_TH=7.03
PRIMER_INTERNAL_84_SELF_END_TH=0.00
PRIMER_INTERNAL_84_HAIRPIN_TH=43.79
PRIMER_INTERNAL_85_PENALTY=6.841708
PRIMER_INTERNAL_85_SEQUENCE=CCCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_85=78,26
PRIMER_INTERNAL_85_TM=59.158
PRIMER_INTERNAL_85_GC_PERCENT=53.846
PRIMER_INTERNAL_85_SELF_ANY_TH=18.40
PRIMER_INTERNAL_85_SELF_END_TH=11.69
PRIMER_INTERNAL_85_HAIRPIN_TH=43.02
PRIMER_INTERNAL_86_PENALTY=6.895596
PRIMER_INTERNAL_86_SEQUENCE=GGCCATTCGATCCCGACCCAAATCG
PRIMER_INTERNAL_86=67,25
PRIMER_INTERNAL_86_TM=61.896
PRIMER_INTERNAL_86_GC_PERCENT=60.000
PRIMER_INTERNAL_86_SELF_ANY_TH=0.00
PRIMER_INTERNAL_86_SELF_END_TH=0.00
PRIMER_INTERNAL_86_HAIRPIN_TH=41.60
PRIMER_INTERNAL_87_PENALTY=6.905174
PRIMER_INTERNAL_87_SEQUENCE=CGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_87=74,25
PRIMER_INTERNAL_87_TM=58.095
PRIMER_INTERNAL_87_GC_PERCENT=52.000
PRIMER_INTERNAL_87_SELF_ANY_TH=0.00
PRIMER_INTERNAL_87_SELF_END_TH=0.00
PRIMER_INTERNAL_87_HAIRPIN_TH=41.28
PRIMER_INTERNAL_88_PENALTY=6.905408
PRIMER_INTERNAL_88_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_88=58,26
PRIMER_INTERNAL_88_TM=59.095
PRIMER_INTERNAL_88_GC_PERCENT=53.846
PRIMER_INTERNAL_88_SELF_ANY_TH=7.03
PRIMER_INTERNAL_88_SELF_END_TH=0.00
PRIMER_INTERNAL_88_HAIRPIN_TH=41.60
PRIMER_INTERNAL_89_PENALTY=6.917157
PRIMER_INTERNAL_89_SEQUENCE=ACACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_89=37,26
PRIMER_INTERNAL_89_TM=59.083
PRIMER_INTERNAL_89_GC_PERCENT=46.154
PRIMER_INTERNAL_89_SELF_ANY_TH=0.00
PRIMER_INTERNAL_89_SELF_END_TH=0.00
PRIMER_INTERNAL_89_HAIRPIN_TH=0.00
PRIMER_INTERNAL_90_PENALTY=6.956454
PRIMER_INTERNAL_90_SEQUENCE=AGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_90=10,24
PRIMER_INTERNAL_90_TM=57.044
PRIMER_INTERNAL_90_GC_PERCENT=50.000
PRIMER_INTERNAL_90_SELF_ANY_TH=0.00
PRIMER_INTERNAL_90_SELF_END_TH=0.00
PRIMER_INTERNAL_90_HAIRPIN_TH=36.06
PRIMER_INTERNAL_91_PENALTY=6.975446
PRIMER_INTERNAL_91_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_91=73,26
PRIMER_INTERNAL_91_TM=59.025
PRIMER_INTERNAL_91_GC_PERCENT=50.000
PRIMER_INTERNAL_91_SELF_ANY_TH=0.00
PRIMER_INTERNAL_91_SELF_END_TH=0.00
PRIMER_INTERNAL_91_HAIRPIN_TH=38.51
PRIMER_INTERNAL_92_PENALTY=7.015932
PRIMER_INTERNAL_92_SEQUENCE=CCCGACCCAAATCGATAGCTACGATGA
PRIMER_INTERNAL_92=78,27
PRIMER_INTERNAL_92_TM=60.016
PRIMER_INTERNAL_92_GC_PERCENT=51.852
PRIMER_INTERNAL_92_SELF_ANY_TH=18.40
PRIMER_INTERNAL_92_SELF_END_TH=5.09
PRIMER_INTERNAL_92_HAIRPIN_TH=43.02
PRIMER_INTERNAL_93_PENALTY=7.015932
PRIMER_INTERNAL_93_SEQUENCE=TCCCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_93=77,27
PRIMER_INTERNAL_93_TM=60.016
PRIMER_INTERNAL_93_GC_PERCENT=51.852
PRIMER_INTERNAL_93_SELF_ANY_TH=18.40
PRIMER_INTERNAL_93_SELF_END_TH=11.69
PRIMER_INTERNAL_93_HAIRPIN_TH=43.02
PRIMER_INTERNAL_94_PENALTY=7.051167
PRIMER_INTERNAL_94_SEQUENCE=TTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_94=61,25
PRIMER_INTERNAL_94_TM=62.051
PRIMER_INTERNAL_94_GC_PERCENT=60.000
PRIMER_INTERNAL_94_SELF_ANY_TH=7.03
PRIMER_INTERNAL_94_SELF_END_TH=0.00
PRIMER_INTERNAL_94_HAIRPIN_TH=41.60
PRIMER_INTERNAL_95_PENALTY=7.056335
PRIMER_INTERNAL_95_SEQUENCE=GATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_95=75,26
PRIMER_INTERNAL_95_TM=58.944
PRIMER_INTERNAL_95_GC_PERCENT=53.846
PRIMER_INTERNAL_95_SELF_ANY_TH=0.00
PRIMER_INTERNAL_95_SELF_END_TH=0.00
PRIMER_INTERNAL_95_HAIRPIN_TH=41.28
PRIMER_INTERNAL_96_PENALTY=7.056335
PRIMER_INTERNAL_96_SEQUENCE=CGATCCCGACCCAAATCGATAGCTAC
PRIMER_INTERNAL_96=74,26
PRIMER_INTERNAL_96_TM=58.944
PRIMER_INTERNAL_96_GC_PERCENT=53.846
PRIMER_INTERNAL_96_SELF_ANY_TH=0.00
PRIMER_INTERNAL_96_SELF_END_TH=0.00
PRIMER_INTERNAL_96_HAIRPIN_TH=41.28
PRIMER_INTERNAL_97_PENALTY=7.066919
PRIMER_INTERNAL_97_SEQUENCE=CACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_97=38,25
PRIMER_INTERNAL_97_TM=57.933
PRIMER_INTERNAL_97_GC_PERCENT=48.000
PRIMER_INTERNAL_97_SELF_ANY_TH=0.00
PRIMER_INTERNAL_97_SELF_END_TH=0.00
PRIMER_INTERNAL_97_HAIRPIN_TH=0.00
PRIMER_INTERNAL_98_PENALTY=7.070364
PRIMER_INTERNAL_98_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_98=56,25
PRIMER_INTERNAL_98_TM=57.930
PRIMER_INTERNAL_98_GC_PERCENT=52.000
PRIMER_INTERNAL_98_SELF_ANY_TH=7.03
PRIMER_INTERNAL_98_SELF_END_TH=0.00
PRIMER_INTERNAL_98_HAIRPIN_TH=38.81
PRIMER_INTERNAL_99_PENALTY=7.094172
PRIMER_INTERNAL_99_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_99=54,27
PRIMER_INTERNAL_99_TM=59.906
PRIMER_INTERNAL_99_GC_PERCENT=51.852
PRIMER_INTERNAL_99_SELF_ANY_TH=7.03
PRIMER_INTERNAL_99_SELF_END_TH=0.00
PRIMER_INTERNAL_99_HAIRPIN_TH=38.81
PRIMER_INTERNAL_100_PENALTY=7.124042
PRIMER_INTERNAL_100_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_100=71,26
PRIMER_INTERNAL_100_TM=58.876
PRIMER_INTERNAL_100_GC_PERCENT=50.000
PRIMER_INTERNAL_100_SELF_ANY_TH=0.00
PRIMER_INTERNAL_100_SELF_END_TH=0.00
PRIMER_INTERNAL_100_HAIRPIN_TH=41.68
PRIMER_INTERNAL_101_PENALTY=7.139284
PRIMER_INTERNAL_101_SEQUENCE=CACACACACACACAGCACACAGGTATT
PRIMER_INTERNAL_101=36,27
PRIMER_INTERNAL_101_TM=59.861
PRIMER_INTERNAL_101_GC_PERCENT=48.148
PRIMER_INTERNAL_101_SELF_ANY_TH=0.00
PRIMER_INTERNAL_101_SELF_END_TH=0.00
PRIMER_INTERNAL_101_HAIRPIN_TH=0.00
PRIMER_INTERNAL_102_PENALTY=7.145199
PRIMER_INTERNAL_102_SEQUENCE=GCCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_102=68,26
PRIMER_INTERNAL_102_TM=61.145
PRIMER_INTERNAL_102_GC_PERCENT=53.846
PRIMER_INTERNAL_102_SELF_ANY_TH=2.97
PRIMER_INTERNAL_102_SELF_END_TH=0.00
PRIMER_INTERNAL_102_HAIRPIN_TH=41.68
PRIMER_INTERNAL_103_PENALTY=7.192525
PRIMER_INTERNAL_103_SEQUENCE=GATCCCGACCCAAATCGATAGCTACGA
PRIMER_INTERNAL_103=75,27
PRIMER_INTERNAL_103_TM=59.807
PRIMER_INTERNAL_103_GC_PERCENT=51.852
PRIMER_INTERNAL_103_SELF_ANY_TH=12.68
PRIMER_INTERNAL_103_SELF_END_TH=12.68
PRIMER_INTERNAL_103_HAIRPIN_TH=41.28
PRIMER_INTERNAL_104_PENALTY=7.192525
PRIMER_INTERNAL_104_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTAC
PRIMER_INTERNAL_104=73,27
PRIMER_INTERNAL_104_TM=59.807
PRIMER_INTERNAL_104_GC_PERCENT=51.852
PRIMER_INTERNAL_104_SELF_ANY_TH=0.00
PRIMER_INTERNAL_104_SELF_END_TH=0.00
PRIMER_INTERNAL_104_HAIRPIN_TH=38.51
PRIMER_INTERNAL_105_PENALTY=7.193259
PRIMER_INTERNAL_105_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCC
PRIMER_INTERNAL_105=55,26
PRIMER_INTERNAL_105_TM=58.807
PRIMER_INTERNAL_105_GC_PERCENT=53.846
PRIMER_INTERNAL_105_SELF_ANY_TH=7.03
PRIMER_INTERNAL_105_SELF_END_TH=0.00
PRIMER_INTERNAL_105_HAIRPIN_TH=38.81
PRIMER_INTERNAL_106_PENALTY=7.239217
PRIMER_INTERNAL_106_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGA
PRIMER_INTERNAL_106=52,25
PRIMER_INTERNAL_106_TM=57.761
PRIMER_INTERNAL_106_GC_PERCENT=48.000
PRIMER_INTERNAL_106_SELF_ANY_TH=7.03
PRIMER_INTERNAL_106_SELF_END_TH=0.00
PRIMER_INTERNAL_106_HAIRPIN_TH=36.70
PRIMER_INTERNAL_107_PENALTY=7.243997
PRIMER_INTERNAL_107_SEQUENCE=TGGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_107=65,25
PRIMER_INTERNAL_107_TM=62.244
PRIMER_INTERNAL_107_GC_PERCENT=56.000
PRIMER_INTERNAL_107_SELF_ANY_TH=0.00
PRIMER_INTERNAL_107_SELF_END_TH=0.00
PRIMER_INTERNAL_107_HAIRPIN_TH=41.29
PRIMER_INTERNAL_108_PENALTY=7.246964
PRIMER_INTERNAL_108_SEQUENCE=ACACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_108=43,26
PRIMER_INTERNAL_108_TM=58.753
PRIMER_INTERNAL_108_GC_PERCENT=50.000
PRIMER_INTERNAL_108_SELF_ANY_TH=0.00
PRIMER_INTERNAL_108_SELF_END_TH=0.00
PRIMER_INTERNAL_108_HAIRPIN_TH=38.74
PRIMER_INTERNAL_109_PENALTY=7.250024
PRIMER_INTERNAL_109_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGCT
PRIMER_INTERNAL_109=71,27
PRIMER_INTERNAL_109_TM=59.750
PRIMER_INTERNAL_109_GC_PERCENT=48.148
PRIMER_INTERNAL_109_SELF_ANY_TH=0.00
PRIMER_INTERNAL_109_SELF_END_TH=0.00
PRIMER_INTERNAL_109_HAIRPIN_TH=41.68
PRIMER_INTERNAL_110_PENALTY=7.293083
PRIMER_INTERNAL_110_SEQUENCE=CACACAGGTATTAGTGGGCCATTCG
PRIMER_INTERNAL_110=51,25
PRIMER_INTERNAL_110_TM=57.707
PRIMER_INTERNAL_110_GC_PERCENT=52.000
PRIMER_INTERNAL_110_SELF_ANY_TH=7.03
PRIMER_INTERNAL_110_SELF_END_TH=0.00
PRIMER_INTERNAL_110_HAIRPIN_TH=36.70
PRIMER_INTERNAL_111_PENALTY=7.342607
PRIMER_INTERNAL_111_SEQUENCE=CATTCGATCCCGACCCAAATCGATAGC
PRIMER_INTERNAL_111=70,27
PRIMER_INTERNAL_111_TM=59.657
PRIMER_INTERNAL_111_GC_PERCENT=51.852
PRIMER_INTERNAL_111_SELF_ANY_TH=0.00
PRIMER_INTERNAL_111_SELF_END_TH=0.00
PRIMER_INTERNAL_111_HAIRPIN_TH=41.68
PRIMER_INTERNAL_112_PENALTY=7.354919
PRIMER_INTERNAL_112_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGA
PRIMER_INTERNAL_112=51,26
PRIMER_INTERNAL_112_TM=58.645
PRIMER_INTERNAL_112_GC_PERCENT=50.000
PRIMER_INTERNAL_112_SELF_ANY_TH=7.03
PRIMER_INTERNAL_112_SELF_END_TH=0.00
PRIMER_INTERNAL_112_HAIRPIN_TH=36.70
PRIMER_INTERNAL_113_PENALTY=7.401440
PRIMER_INTERNAL_113_SEQUENCE=CCATTCGATCCCGACCCAAATCGATA
PRIMER_INTERNAL_113=69,26
PRIMER_INTERNAL_113_TM=58.599
PRIMER_INTERNAL_113_GC_PERCENT=50.000
PRIMER_INTERNAL_113_SELF_ANY_TH=0.00
PRIMER_INTERNAL_113_SELF_END_TH=0.00
PRIMER_INTERNAL_113_HAIRPIN_TH=41.68
PRIMER_INTERNAL_114_PENALTY=7.408271
PRIMER_INTERNAL_114_SEQUENCE=CACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_114=44,25
PRIMER_INTERNAL_114_TM=57.592
PRIMER_INTERNAL_114_GC_PERCENT=52.000
PRIMER_INTERNAL_114_SELF_ANY_TH=0.00
PRIMER_INTERNAL_114_SELF_END_TH=0.00
PRIMER_INTERNAL_114_HAIRPIN_TH=38.74
PRIMER_INTERNAL_115_PENALTY=7.451841
PRIMER_INTERNAL_115_SEQUENCE=CACACACAGCACACAGGTATTAGTGGG
PRIMER_INTERNAL_115=42,27
PRIMER_INTERNAL_115_TM=59.548
PRIMER_INTERNAL_115_GC_PERCENT=51.852
PRIMER_INTERNAL_115_SELF_ANY_TH=0.00
PRIMER_INTERNAL_115_SELF_END_TH=0.00
PRIMER_INTERNAL_115_HAIRPIN_TH=38.74
PRIMER_INTERNAL_116_PENALTY=7.534114
PRIMER_INTERNAL_116_SEQUENCE=CCGACCCAAATCGATAGCTACGATG
PRIMER_INTERNAL_116=79,25
PRIMER_INTERNAL_116_TM=57.466
PRIMER_INTERNAL_116_GC_PERCENT=52.000
PRIMER_INTERNAL_116_SELF_ANY_TH=18.40
PRIMER_INTERNAL_116_SELF_END_TH=11.69
PRIMER_INTERNAL_116_HAIRPIN_TH=43.02
PRIMER_INTERNAL_117_PENALTY=7.537719
PRIMER_INTERNAL_117_SEQUENCE=CGACCCAAATCGATAGCTACGATGACG
PRIMER_INTERNAL_117=80,27
PRIMER_INTERNAL_117_TM=59.462
PRIMER_INTERNAL_117_GC_PERCENT=51.852
PRIMER_INTERNAL_117_SELF_ANY_TH=18.40
PRIMER_INTERNAL_117_SELF_END_TH=13.91
PRIMER_INTERNAL_117_HAIRPIN_TH=43.02
PRIMER_INTERNAL_118_PENALTY=7.602591
PRIMER_INTERNAL_118_SEQUENCE=CCGACCCAAATCGATAGCTACGATGA
PRIMER_INTERNAL_118=79,26
PRIMER_INTERNAL_118_TM=58.397
PRIMER_INTERNAL_118_GC_PERCENT=50.000
PRIMER_INTERNAL_118_SELF_ANY_TH=18.40
PRIMER_INTERNAL_118_SELF_END_TH=5.09
PRIMER_INTERNAL_118_HAIRPIN_TH=43.02
PRIMER_INTERNAL_119_PENALTY=7.672898
PRIMER_INTERNAL_119_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGAT
PRIMER_INTERNAL_119=76,27
PRIMER_INTERNAL_119_TM=59.327
PRIMER_INTERNAL_119_GC_PERCENT=48.148
PRIMER_INTERNAL_119_SELF_ANY_TH=14.48
PRIMER_INTERNAL_119_SELF_END_TH=14.48
PRIMER_INTERNAL_119_HAIRPIN_TH=46.54
PRIMER_INTERNAL_120_PENALTY=7.693260
PRIMER_INTERNAL_120_SEQUENCE=GCCATTCGATCCCGACCCAAATCGATA
PRIMER_INTERNAL_120=68,27
PRIMER_INTERNAL_120_TM=60.693
PRIMER_INTERNAL_120_GC_PERCENT=51.852
PRIMER_INTERNAL_120_SELF_ANY_TH=0.00
PRIMER_INTERNAL_120_SELF_END_TH=0.00
PRIMER_INTERNAL_120_HAIRPIN_TH=41.68
PRIMER_INTERNAL_121_PENALTY=7.693499
PRIMER_INTERNAL_121_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_121=58,27
PRIMER_INTERNAL_121_TM=60.693
PRIMER_INTERNAL_121_GC_PERCENT=55.556
PRIMER_INTERNAL_121_SELF_ANY_TH=7.03
PRIMER_INTERNAL_121_SELF_END_TH=0.00
PRIMER_INTERNAL_121_HAIRPIN_TH=41.60
PRIMER_INTERNAL_122_PENALTY=7.693499
PRIMER_INTERNAL_122_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_122=57,27
PRIMER_INTERNAL_122_TM=60.693
PRIMER_INTERNAL_122_GC_PERCENT=55.556
PRIMER_INTERNAL_122_SELF_ANY_TH=7.03
PRIMER_INTERNAL_122_SELF_END_TH=0.00
PRIMER_INTERNAL_122_HAIRPIN_TH=41.60
PRIMER_INTERNAL_123_PENALTY=7.694218
PRIMER_INTERNAL_123_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCCG
PRIMER_INTERNAL_123=55,27
PRIMER_INTERNAL_123_TM=60.694
PRIMER_INTERNAL_123_GC_PERCENT=55.556
PRIMER_INTERNAL_123_SELF_ANY_TH=7.03
PRIMER_INTERNAL_123_SELF_END_TH=0.00
PRIMER_INTERNAL_123_HAIRPIN_TH=39.49
PRIMER_INTERNAL_124_PENALTY=7.696389
PRIMER_INTERNAL_124_SEQUENCE=ACACACACACACACAGCACACAGGTAT
PRIMER_INTERNAL_124=35,27
PRIMER_INTERNAL_124_TM=60.696
PRIMER_INTERNAL_124_GC_PERCENT=48.148
PRIMER_INTERNAL_124_SELF_ANY_TH=0.00
PRIMER_INTERNAL_124_SELF_END_TH=0.00
PRIMER_INTERNAL_124_HAIRPIN_TH=0.00
PRIMER_INTERNAL_125_PENALTY=7.723400
PRIMER_INTERNAL_125_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCTA
PRIMER_INTERNAL_125=72,27
PRIMER_INTERNAL_125_TM=59.277
PRIMER_INTERNAL_125_GC_PERCENT=48.148
PRIMER_INTERNAL_125_SELF_ANY_TH=0.00
PRIMER_INTERNAL_125_SELF_END_TH=0.00
PRIMER_INTERNAL_125_HAIRPIN_TH=41.68
PRIMER_INTERNAL_126_PENALTY=7.753661
PRIMER_INTERNAL_126_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_126=54,26
PRIMER_INTERNAL_126_TM=58.246
PRIMER_INTERNAL_126_GC_PERCENT=50.000
PRIMER_INTERNAL_126_SELF_ANY_TH=7.03
PRIMER_INTERNAL_126_SELF_END_TH=5.96
PRIMER_INTERNAL_126_HAIRPIN_TH=0.00
PRIMER_INTERNAL_127_PENALTY=7.785822
PRIMER_INTERNAL_127_SEQUENCE=CGATCCCGACCCAAATCGATAGCTACG
PRIMER_INTERNAL_127=74,27
PRIMER_INTERNAL_127_TM=60.786
PRIMER_INTERNAL_127_GC_PERCENT=55.556
PRIMER_INTERNAL_127_SELF_ANY_TH=0.00
PRIMER_INTERNAL_127_SELF_END_TH=0.00
PRIMER_INTERNAL_127_HAIRPIN_TH=41.28
PRIMER_INTERNAL_128_PENALTY=7.802259
PRIMER_INTERNAL_128_SEQUENCE=CCGACCCAAATCGATAGCTACGATGAC
PRIMER_INTERNAL_128=79,27
PRIMER_INTERNAL_128_TM=59.198
PRIMER_INTERNAL_128_GC_PERCENT=51.852
PRIMER_INTERNAL_128_SELF_ANY_TH=18.40
PRIMER_INTERNAL_128_SELF_END_TH=9.31
PRIMER_INTERNAL_128_HAIRPIN_TH=43.02
PRIMER_INTERNAL_129_PENALTY=7.812760
PRIMER_INTERNAL_129_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_129=56,27
PRIMER_INTERNAL_129_TM=60.813
PRIMER_INTERNAL_129_GC_PERCENT=51.852
PRIMER_INTERNAL_129_SELF_ANY_TH=7.03
PRIMER_INTERNAL_129_SELF_END_TH=0.00
PRIMER_INTERNAL_129_HAIRPIN_TH=39.49
PRIMER_INTERNAL_130_PENALTY=7.812835
PRIMER_INTERNAL_130_SEQUENCE=CCATTCGATCCCGACCCAAATCGATAG
PRIMER_INTERNAL_130=69,27
PRIMER_INTERNAL_130_TM=59.187
PRIMER_INTERNAL_130_GC_PERCENT=51.852
PRIMER_INTERNAL_130_SELF_ANY_TH=0.00
PRIMER_INTERNAL_130_SELF_END_TH=0.00
PRIMER_INTERNAL_130_HAIRPIN_TH=41.68
PRIMER_INTERNAL_131_PENALTY=7.928092
PRIMER_INTERNAL_131_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_131=53,27
PRIMER_INTERNAL_131_TM=59.072
PRIMER_INTERNAL_131_GC_PERCENT=51.852
PRIMER_INTERNAL_131_SELF_ANY_TH=7.03
PRIMER_INTERNAL_131_SELF_END_TH=5.96
PRIMER_INTERNAL_131_HAIRPIN_TH=29.02
PRIMER_INTERNAL_132_PENALTY=7.938713
PRIMER_INTERNAL_132_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCC
PRIMER_INTERNAL_132=55,25
PRIMER_INTERNAL_132_TM=57.061
PRIMER_INTERNAL_132_GC_PERCENT=52.000
PRIMER_INTERNAL_132_SELF_ANY_TH=7.03
PRIMER_INTERNAL_132_SELF_END_TH=5.96
PRIMER_INTERNAL_132_HAIRPIN_TH=0.00
PRIMER_INTERNAL_133_PENALTY=7.949148
PRIMER_INTERNAL_133_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_133=64,25
PRIMER_INTERNAL_133_TM=62.949
PRIMER_INTERNAL_133_GC_PERCENT=60.000
PRIMER_INTERNAL_133_SELF_ANY_TH=7.03
PRIMER_INTERNAL_133_SELF_END_TH=0.00
PRIMER_INTERNAL_133_HAIRPIN_TH=43.79
PRIMER_INTERNAL_134_PENALTY=7.979746
PRIMER_INTERNAL_134_SEQUENCE=ACACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_134=41,27
PRIMER_INTERNAL_134_TM=59.020
PRIMER_INTERNAL_134_GC_PERCENT=48.148
PRIMER_INTERNAL_134_SELF_ANY_TH=0.00
PRIMER_INTERNAL_134_SELF_END_TH=0.00
PRIMER_INTERNAL_134_HAIRPIN_TH=36.70
PRIMER_INTERNAL_135_PENALTY=7.992102
PRIMER_INTERNAL_135_SEQUENCE=TAGTGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_135=62,25
PRIMER_INTERNAL_135_TM=62.992
PRIMER_INTERNAL_135_GC_PERCENT=60.000
PRIMER_INTERNAL_135_SELF_ANY_TH=7.03
PRIMER_INTERNAL_135_SELF_END_TH=3.99
PRIMER_INTERNAL_135_HAIRPIN_TH=42.67
PRIMER_INTERNAL_136_PENALTY=7.993148
PRIMER_INTERNAL_136_SEQUENCE=ACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_136=43,25
PRIMER_INTERNAL_136_TM=57.007
PRIMER_INTERNAL_136_GC_PERCENT=48.000
PRIMER_INTERNAL_136_SELF_ANY_TH=0.00
PRIMER_INTERNAL_136_SELF_END_TH=0.00
PRIMER_INTERNAL_136_HAIRPIN_TH=36.70
PRIMER_INTERNAL_137_PENALTY=8.038590
PRIMER_INTERNAL_137_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_137=60,26
PRIMER_INTERNAL_137_TM=62.039
PRIMER_INTERNAL_137_GC_PERCENT=57.692
PRIMER_INTERNAL_137_SELF_ANY_TH=7.03
PRIMER_INTERNAL_137_SELF_END_TH=0.00
PRIMER_INTERNAL_137_HAIRPIN_TH=41.60
PRIMER_INTERNAL_138_PENALTY=8.084641
PRIMER_INTERNAL_138_SEQUENCE=CACACACAGCACACAGGTATTAGTGG
PRIMER_INTERNAL_138=42,26
PRIMER_INTERNAL_138_TM=57.915
PRIMER_INTERNAL_138_GC_PERCENT=50.000
PRIMER_INTERNAL_138_SELF_ANY_TH=0.00
PRIMER_INTERNAL_138_SELF_END_TH=0.00
PRIMER_INTERNAL_138_HAIRPIN_TH=36.70
PRIMER_INTERNAL_139_PENALTY=8.098597
PRIMER_INTERNAL_139_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGAT
PRIMER_INTERNAL_139=52,26
PRIMER_INTERNAL_139_TM=57.901
PRIMER_INTERNAL_139_GC_PERCENT=46.154
PRIMER_INTERNAL_139_SELF_ANY_TH=7.03
PRIMER_INTERNAL_139_SELF_END_TH=2.18
PRIMER_INTERNAL_139_HAIRPIN_TH=36.70
PRIMER_INTERNAL_140_PENALTY=8.106799
PRIMER_INTERNAL_140_SEQUENCE=TCAGTAGACATGACACTGACGATGCA
PRIMER_INTERNAL_140=5,26
PRIMER_INTERNAL_140_TM=57.893
PRIMER_INTERNAL_140_GC_PERCENT=46.154
PRIMER_INTERNAL_140_SELF_ANY_TH=13.87
PRIMER_INTERNAL_140_SELF_END_TH=0.00
PRIMER_INTERNAL_140_HAIRPIN_TH=37.56
PRIMER_INTERNAL_141_PENALTY=8.251191
PRIMER_INTERNAL_141_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGAT
PRIMER_INTERNAL_141=51,27
PRIMER_INTERNAL_141_TM=58.749
PRIMER_INTERNAL_141_GC_PERCENT=48.148
PRIMER_INTERNAL_141_SELF_ANY_TH=7.03
PRIMER_INTERNAL_141_SELF_END_TH=2.18
PRIMER_INTERNAL_141_HAIRPIN_TH=36.70
PRIMER_INTERNAL_142_PENALTY=8.275978
PRIMER_INTERNAL_142_SEQUENCE=GTCAGTAGACATGACACTGACGATGCA
PRIMER_INTERNAL_142=4,27
PRIMER_INTERNAL_142_TM=58.724
PRIMER_INTERNAL_142_GC_PERCENT=48.148
PRIMER_INTERNAL_142_SELF_ANY_TH=22.59
PRIMER_INTERNAL_142_SELF_END_TH=0.00
PRIMER_INTERNAL_142_HAIRPIN_TH=46.00
PRIMER_INTERNAL_143_PENALTY=8.300145
PRIMER_INTERNAL_143_SEQUENCE=ACACACACACACAGCACACAGGTATTA
PRIMER_INTERNAL_143=37,27
PRIMER_INTERNAL_143_TM=58.700
PRIMER_INTERNAL_143_GC_PERCENT=44.444
PRIMER_INTERNAL_143_SELF_ANY_TH=0.00
PRIMER_INTERNAL_143_SELF_END_TH=0.00
PRIMER_INTERNAL_143_HAIRPIN_TH=0.00
PRIMER_INTERNAL_144_PENALTY=8.330872
PRIMER_INTERNAL_144_SEQUENCE=AGTAGACATGACACTGACGATGCAGA
PRIMER_INTERNAL_144=7,26
PRIMER_INTERNAL_144_TM=57.669
PRIMER_INTERNAL_144_GC_PERCENT=46.154
PRIMER_INTERNAL_144_SELF_ANY_TH=0.00
PRIMER_INTERNAL_144_SELF_END_TH=0.00
PRIMER_INTERNAL_144_HAIRPIN_TH=36.06
PRIMER_INTERNAL_145_PENALTY=8.376203
PRIMER_INTERNAL_145_SEQUENCE=GTAGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_145=8,26
PRIMER_INTERNAL_145_TM=57.624
PRIMER_INTERNAL_145_GC_PERCENT=50.000
PRIMER_INTERNAL_145_SELF_ANY_TH=0.00
PRIMER_INTERNAL_145_SELF_END_TH=0.00
PRIMER_INTERNAL_145_HAIRPIN_TH=36.06
PRIMER_INTERNAL_146_PENALTY=8.376203
PRIMER_INTERNAL_146_SEQUENCE=GTCAGTAGACATGACACTGACGATGC
PRIMER_INTERNAL_146=4,26
PRIMER_INTERNAL_146_TM=57.624
PRIMER_INTERNAL_146_GC_PERCENT=50.000
PRIMER_INTERNAL_146_SELF_ANY_TH=22.59
PRIMER_INTERNAL_146_SELF_END_TH=10.24
PRIMER_INTERNAL_146_HAIRPIN_TH=46.00
PRIMER_INTERNAL_147_PENALTY=8.379879
PRIMER_INTERNAL_147_SEQUENCE=CAGTAGACATGACACTGACGATGCAG
PRIMER_INTERNAL_147=6,26
PRIMER_INTERNAL_147_TM=57.620
PRIMER_INTERNAL_147_GC_PERCENT=50.000
PRIMER_INTERNAL_147_SELF_ANY_TH=12.06
PRIMER_INTERNAL_147_SELF_END_TH=0.00
PRIMER_INTERNAL_147_HAIRPIN_TH=37.39
PRIMER_INTERNAL_148_PENALTY=8.418488
PRIMER_INTERNAL_148_SEQUENCE=CACACACACACAGCACACAGGTATTA
PRIMER_INTERNAL_148=38,26
PRIMER_INTERNAL_148_TM=57.582
PRIMER_INTERNAL_148_GC_PERCENT=46.154
PRIMER_INTERNAL_148_SELF_ANY_TH=0.00
PRIMER_INTERNAL_148_SELF_END_TH=0.00
PRIMER_INTERNAL_148_HAIRPIN_TH=0.00
PRIMER_INTERNAL_149_PENALTY=8.423914
PRIMER_INTERNAL_149_SEQUENCE=GACCCAAATCGATAGCTACGATGACG
PRIMER_INTERNAL_149=81,26
PRIMER_INTERNAL_149_TM=57.576
PRIMER_INTERNAL_149_GC_PERCENT=50.000
PRIMER_INTERNAL_149_SELF_ANY_TH=18.40
PRIMER_INTERNAL_149_SELF_END_TH=12.20
PRIMER_INTERNAL_149_HAIRPIN_TH=43.02
PRIMER_INTERNAL_150_PENALTY=8.423914
PRIMER_INTERNAL_150_SEQUENCE=CGACCCAAATCGATAGCTACGATGAC
PRIMER_INTERNAL_150=80,26
PRIMER_INTERNAL_150_TM=57.576
PRIMER_INTERNAL_150_GC_PERCENT=50.000
PRIMER_INTERNAL_150_SELF_ANY_TH=18.40
PRIMER_INTERNAL_150_SELF_END_TH=9.31
PRIMER_INTERNAL_150_HAIRPIN_TH=43.02
PRIMER_INTERNAL_151_PENALTY=8.452836
PRIMER_INTERNAL_151_SEQUENCE=CATTCGATCCCGACCCAAATCGATAG
PRIMER_INTERNAL_151=70,26
PRIMER_INTERNAL_151_TM=57.547
PRIMER_INTERNAL_151_GC_PERCENT=50.000
PRIMER_INTERNAL_151_SELF_ANY_TH=0.00
PRIMER_INTERNAL_151_SELF_END_TH=0.00
PRIMER_INTERNAL_151_HAIRPIN_TH=41.68
PRIMER_INTERNAL_152_PENALTY=8.463323
PRIMER_INTERNAL_152_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGATC
PRIMER_INTERNAL_152=52,27
PRIMER_INTERNAL_152_TM=58.537
PRIMER_INTERNAL_152_GC_PERCENT=48.148
PRIMER_INTERNAL_152_SELF_ANY_TH=7.03
PRIMER_INTERNAL_152_SELF_END_TH=0.00
PRIMER_INTERNAL_152_HAIRPIN_TH=36.70
PRIMER_INTERNAL_153_PENALTY=8.486477
PRIMER_INTERNAL_153_SEQUENCE=CAGTAGACATGACACTGACGATGCAGA
PRIMER_INTERNAL_153=6,27
PRIMER_INTERNAL_153_TM=58.514
PRIMER_INTERNAL_153_GC_PERCENT=48.148
PRIMER_INTERNAL_153_SELF_ANY_TH=12.06
PRIMER_INTERNAL_153_SELF_END_TH=0.00
PRIMER_INTERNAL_153_HAIRPIN_TH=37.39
PRIMER_INTERNAL_154_PENALTY=8.486477
PRIMER_INTERNAL_154_SEQUENCE=TCAGTAGACATGACACTGACGATGCAG
PRIMER_INTERNAL_154=5,27
PRIMER_INTERNAL_154_TM=58.514
PRIMER_INTERNAL_154_GC_PERCENT=48.148
PRIMER_INTERNAL_154_SELF_ANY_TH=13.87
PRIMER_INTERNAL_154_SELF_END_TH=0.00
PRIMER_INTERNAL_154_HAIRPIN_TH=37.56
PRIMER_INTERNAL_155_PENALTY=8.487256
PRIMER_INTERNAL_155_SEQUENCE=AGTAGACATGACACTGACGATGCAGAC
PRIMER_INTERNAL_155=7,27
PRIMER_INTERNAL_155_TM=58.513
PRIMER_INTERNAL_155_GC_PERCENT=48.148
PRIMER_INTERNAL_155_SELF_ANY_TH=0.00
PRIMER_INTERNAL_155_SELF_END_TH=0.00
PRIMER_INTERNAL_155_HAIRPIN_TH=36.06
PRIMER_INTERNAL_156_PENALTY=8.514997
PRIMER_INTERNAL_156_SEQUENCE=ACACACACACAGCACACAGGTATTAGT
PRIMER_INTERNAL_156=39,27
PRIMER_INTERNAL_156_TM=58.485
PRIMER_INTERNAL_156_GC_PERCENT=44.444
PRIMER_INTERNAL_156_SELF_ANY_TH=0.00
PRIMER_INTERNAL_156_SELF_END_TH=0.00
PRIMER_INTERNAL_156_HAIRPIN_TH=0.00
PRIMER_INTERNAL_157_PENALTY=8.547914
PRIMER_INTERNAL_157_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_157=59,27
PRIMER_INTERNAL_157_TM=61.548
PRIMER_INTERNAL_157_GC_PERCENT=55.556
PRIMER_INTERNAL_157_SELF_ANY_TH=7.03
PRIMER_INTERNAL_157_SELF_END_TH=0.00
PRIMER_INTERNAL_157_HAIRPIN_TH=41.60
PRIMER_INTERNAL_158_PENALTY=8.589885
PRIMER_INTERNAL_158_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATC
PRIMER_INTERNAL_158=53,26
PRIMER_INTERNAL_158_TM=57.410
PRIMER_INTERNAL_158_GC_PERCENT=50.000
PRIMER_INTERNAL_158_SELF_ANY_TH=7.03
PRIMER_INTERNAL_158_SELF_END_TH=0.00
PRIMER_INTERNAL_158_HAIRPIN_TH=29.02
PRIMER_INTERNAL_159_PENALTY=8.642220
PRIMER_INTERNAL_159_SEQUENCE=ACACACACAGCACACAGGTATTAGTG
PRIMER_INTERNAL_159=41,26
PRIMER_INTERNAL_159_TM=57.358
PRIMER_INTERNAL_159_GC_PERCENT=46.154
PRIMER_INTERNAL_159_SELF_ANY_TH=0.00
PRIMER_INTERNAL_159_SELF_END_TH=0.00
PRIMER_INTERNAL_159_HAIRPIN_TH=39.84
PRIMER_INTERNAL_160_PENALTY=8.642220
PRIMER_INTERNAL_160_SEQUENCE=CACACACACAGCACACAGGTATTAGT
PRIMER_INTERNAL_160=40,26
PRIMER_INTERNAL_160_TM=57.358
PRIMER_INTERNAL_160_GC_PERCENT=46.154
PRIMER_INTERNAL_160_SELF_ANY_TH=0.00
PRIMER_INTERNAL_160_SELF_END_TH=0.00
PRIMER_INTERNAL_160_HAIRPIN_TH=0.00
PRIMER_INTERNAL_161_PENALTY=8.642220
PRIMER_INTERNAL_161_SEQUENCE=ACACACACACAGCACACAGGTATTAG
PRIMER_INTERNAL_161=39,26
PRIMER_INTERNAL_161_TM=57.358
PRIMER_INTERNAL_161_GC_PERCENT=46.154
PRIMER_INTERNAL_161_SELF_ANY_TH=0.00
PRIMER_INTERNAL_161_SELF_END_TH=0.00
PRIMER_INTERNAL_161_HAIRPIN_TH=0.00
PRIMER_INTERNAL_162_PENALTY=8.687221
PRIMER_INTERNAL_162_SEQUENCE=TGGGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_162=65,26
PRIMER_INTERNAL_162_TM=62.687
PRIMER_INTERNAL_162_GC_PERCENT=57.692
PRIMER_INTERNAL_162_SELF_ANY_TH=0.00
PRIMER_INTERNAL_162_SELF_END_TH=0.00
PRIMER_INTERNAL_162_HAIRPIN_TH=41.29
PRIMER_INTERNAL_163_PENALTY=8.713667
PRIMER_INTERNAL_163_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGA
PRIMER_INTERNAL_163=67,26
PRIMER_INTERNAL_163_TM=62.714
PRIMER_INTERNAL_163_GC_PERCENT=57.692
PRIMER_INTERNAL_163_SELF_ANY_TH=0.00
PRIMER_INTERNAL_163_SELF_END_TH=0.00
PRIMER_INTERNAL_163_HAIRPIN_TH=42.01
PRIMER_INTERNAL_164_PENALTY=8.787348
PRIMER_INTERNAL_164_SEQUENCE=CACACACACAGCACACAGGTATTAGTG
PRIMER_INTERNAL_164=40,27
PRIMER_INTERNAL_164_TM=58.213
PRIMER_INTERNAL_164_GC_PERCENT=48.148
PRIMER_INTERNAL_164_SELF_ANY_TH=0.00
PRIMER_INTERNAL_164_SELF_END_TH=0.00
PRIMER_INTERNAL_164_HAIRPIN_TH=39.84
PRIMER_INTERNAL_165_PENALTY=8.787348
PRIMER_INTERNAL_165_SEQUENCE=CACACACACACAGCACACAGGTATTAG
PRIMER_INTERNAL_165=38,27
PRIMER_INTERNAL_165_TM=58.213
PRIMER_INTERNAL_165_GC_PERCENT=48.148
PRIMER_INTERNAL_165_SELF_ANY_TH=0.00
PRIMER_INTERNAL_165_SELF_END_TH=0.00
PRIMER_INTERNAL_165_HAIRPIN_TH=0.00
PRIMER_INTERNAL_166_PENALTY=8.904972
PRIMER_INTERNAL_166_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_166=64,26
PRIMER_INTERNAL_166_TM=62.905
PRIMER_INTERNAL_166_GC_PERCENT=57.692
PRIMER_INTERNAL_166_SELF_ANY_TH=7.03
PRIMER_INTERNAL_166_SELF_END_TH=0.00
PRIMER_INTERNAL_166_HAIRPIN_TH=43.79
PRIMER_INTERNAL_167_PENALTY=9.679371
PRIMER_INTERNAL_167_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGAT
PRIMER_INTERNAL_167=67,27
PRIMER_INTERNAL_167_TM=62.679
PRIMER_INTERNAL_167_GC_PERCENT=55.556
PRIMER_INTERNAL_167_SELF_ANY_TH=0.00
PRIMER_INTERNAL_167_SELF_END_TH=0.00
PRIMER_INTERNAL_167_HAIRPIN_TH=41.68
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
P3_FILE_FLAG=1
SEQUENCE_ID=pick_primer_list_and_file_flag_th_4
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=33
PRIMER_RIGHT_NUM_RETURNED=33
PRIMER_INTERNAL_NUM_RETURNED=73
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_LEFT_0=118,20
PRIMER_RIGHT_0=137,20
PRIMER_INTERNAL_0=125,20
PRIMER_LEFT_0_TM=58.514
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=59.906
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_1_PENALTY=1.160593
PRIMER_RIGHT_1_PENALTY=1.160593
PRIMER_INTERNAL_1_PENALTY=0.301069
PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_1=122,19
PRIMER_RIGHT_1=140,19
PRIMER_INTERNAL_1=126,20
PRIMER_LEFT_1_TM=59.161
PRIMER_RIGHT_1_TM=59.161
PRIMER_INTERNAL_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_SELF_ANY_TH=7.82
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=37.44
PRIMER_INTERNAL_1_HAIRPIN_TH=36.42
PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_1_END_STABILITY=5.0300
PRIMER_RIGHT_1_END_STABILITY=2.4400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_2_PENALTY=1.656085
PRIMER_RIGHT_2_PENALTY=1.656085
PRIMER_INTERNAL_2_PENALTY=0.900305
PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA
PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG
PRIMER_LEFT_2=117,21
PRIMER_RIGHT_2=137,21
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=58.344
PRIMER_INTERNAL_2_TM=59.100
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.14
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=5.3400
PRIMER_RIGHT_2_END_STABILITY=1.7500
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=8.6247
PRIMER_LEFT_3_PENALTY=1.695359
PRIMER_RIGHT_3_PENALTY=1.695359
PRIMER_INTERNAL_3_PENALTY=0.956520
PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC
PRIMER_LEFT_3=53,21
PRIMER_RIGHT_3=73,21
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=58.305
PRIMER_RIGHT_3_TM=58.305
PRIMER_INTERNAL_3_TM=59.043
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=36.42
PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.7700
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_4_PENALTY=1.831685
PRIMER_RIGHT_4_PENALTY=1.831685
PRIMER_INTERNAL_4_PENALTY=1.018091
PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA
PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT
PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=84,20
PRIMER_INTERNAL_4=125,21
PRIMER_LEFT_4_TM=57.168
PRIMER_RIGHT_4_TM=57.168
PRIMER_INTERNAL_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=57.143
PRIMER_INTERNAL_4_SELF_ANY_TH=7.82
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=35.45
PRIMER_RIGHT_4_HAIRPIN_TH=31.80
PRIMER_INTERNAL_4_HAIRPIN_TH=36.42
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=2.1500
PRIMER_RIGHT_4_END_STABILITY=4.8400
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.8143
PRIMER_LEFT_5_PENALTY=1.902705
PRIMER_RIGHT_5_PENALTY=1.902705
PRIMER_INTERNAL_5_PENALTY=1.178696
PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA
PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_5=62,20
PRIMER_RIGHT_5=81,20
PRIMER_INTERNAL_5=124,21
PRIMER_LEFT_5_TM=57.097
PRIMER_RIGHT_5_TM=57.097
PRIMER_INTERNAL_5_TM=60.179
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=57.143
PRIMER_INTERNAL_5_SELF_ANY_TH=7.82
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=36.42
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=2.7400
PRIMER_RIGHT_5_END_STABILITY=2.5200
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=29.7752
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_6_PENALTY=1.902705
PRIMER_RIGHT_6_PENALTY=1.902705
PRIMER_INTERNAL_6_PENALTY=1.372876
PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT
PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA
PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_6=61,20
PRIMER_RIGHT_6=80,20
PRIMER_INTERNAL_6=126,21
PRIMER_LEFT_6_TM=57.097
PRIMER_RIGHT_6_TM=57.097
PRIMER_INTERNAL_6_TM=60.373
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=7.82
PRIMER_LEFT_6_SELF_ANY_TH=0.00
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=36.42
PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=3.5500
PRIMER_RIGHT_6_END_STABILITY=2.1000
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.0753
PRIMER_LEFT_7_PENALTY=1.950877
PRIMER_RIGHT_7_PENALTY=1.950877
PRIMER_INTERNAL_7_PENALTY=1.393730
PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG
PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA
PRIMER_LEFT_7=119,19
PRIMER_RIGHT_7=137,19
PRIMER_INTERNAL_7=126,19
PRIMER_LEFT_7_TM=58.049
PRIMER_RIGHT_7_TM=58.049
PRIMER_INTERNAL_7_TM=59.606
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_INTERNAL_7_GC_PERCENT=63.158
PRIMER_INTERNAL_7_SELF_ANY_TH=7.82
PRIMER_LEFT_7_SELF_ANY_TH=0.00
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=0.00
PRIMER_RIGHT_7_HAIRPIN_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=36.42
PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=5.3400
PRIMER_RIGHT_7_END_STABILITY=3.5300
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_8_PENALTY=1.972625
PRIMER_RIGHT_8_PENALTY=1.972625
PRIMER_INTERNAL_8_PENALTY=1.595076
PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA
PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_8=56,21
PRIMER_RIGHT_8=76,21
PRIMER_INTERNAL_8=123,21
PRIMER_LEFT_8_TM=58.027
PRIMER_RIGHT_8_TM=58.027
PRIMER_INTERNAL_8_TM=59.405
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_INTERNAL_8_GC_PERCENT=57.143
PRIMER_INTERNAL_8_SELF_ANY_TH=0.00
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=0.00
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=41.14
PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_LEFT_8_END_STABILITY=3.4100
PRIMER_RIGHT_8_END_STABILITY=3.8600
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=32.2237
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.8066
PRIMER_LEFT_9_PENALTY=1.973945
PRIMER_RIGHT_9_PENALTY=1.973945
PRIMER_INTERNAL_9_PENALTY=2.007622
PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC
PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT
PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_9=55,21
PRIMER_RIGHT_9=75,21
PRIMER_INTERNAL_9=121,22
PRIMER_LEFT_9_TM=58.026
PRIMER_RIGHT_9_TM=58.026
PRIMER_INTERNAL_9_TM=59.992
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_INTERNAL_9_GC_PERCENT=59.091
PRIMER_INTERNAL_9_SELF_ANY_TH=0.00
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=0.00
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=0.00
PRIMER_RIGHT_9_HAIRPIN_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=0.00
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=3.0100
PRIMER_RIGHT_9_END_STABILITY=4.0000
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=27.4392
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=5.5040
PRIMER_LEFT_10_PENALTY=1.973945
PRIMER_RIGHT_10_PENALTY=1.973945
PRIMER_INTERNAL_10_PENALTY=2.235780
PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT
PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC
PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG
PRIMER_LEFT_10=54,21
PRIMER_RIGHT_10=74,21
PRIMER_INTERNAL_10=125,19
PRIMER_LEFT_10_TM=58.026
PRIMER_RIGHT_10_TM=58.026
PRIMER_INTERNAL_10_TM=58.764
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_INTERNAL_10_GC_PERCENT=63.158
PRIMER_INTERNAL_10_SELF_ANY_TH=0.00
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=0.00
PRIMER_RIGHT_10_HAIRPIN_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.14
PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=3.1600
PRIMER_RIGHT_10_END_STABILITY=3.8500
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=19.5725
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=9.9844
PRIMER_LEFT_11_PENALTY=2.120321
PRIMER_RIGHT_11_PENALTY=2.120321
PRIMER_INTERNAL_11_PENALTY=2.240074
PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA
PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT
PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_11=64,21
PRIMER_RIGHT_11=84,21
PRIMER_INTERNAL_11=124,22
PRIMER_LEFT_11_TM=57.880
PRIMER_RIGHT_11_TM=57.880
PRIMER_INTERNAL_11_TM=60.240
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_INTERNAL_11_GC_PERCENT=54.545
PRIMER_INTERNAL_11_SELF_ANY_TH=7.82
PRIMER_LEFT_11_SELF_ANY_TH=0.00
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=35.45
PRIMER_RIGHT_11_HAIRPIN_TH=31.80
PRIMER_INTERNAL_11_HAIRPIN_TH=36.42
PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=2.1500
PRIMER_RIGHT_11_END_STABILITY=3.6000
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8905
PRIMER_LEFT_12_PENALTY=2.142778
PRIMER_RIGHT_12_PENALTY=2.142778
PRIMER_INTERNAL_12_PENALTY=2.299276
PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_12=119,20
PRIMER_RIGHT_12=138,20
PRIMER_INTERNAL_12=122,22
PRIMER_LEFT_12_TM=61.143
PRIMER_RIGHT_12_TM=61.143
PRIMER_INTERNAL_12_TM=60.299
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=59.091
PRIMER_INTERNAL_12_SELF_ANY_TH=0.00
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=0.00
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.14
PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=5.3400
PRIMER_RIGHT_12_END_STABILITY=3.5300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_13_PENALTY=2.299089
PRIMER_RIGHT_13_PENALTY=2.299089
PRIMER_INTERNAL_13_PENALTY=2.427391
PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC
PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_13=123,19
PRIMER_RIGHT_13=141,19
PRIMER_INTERNAL_13=123,22
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.299
PRIMER_INTERNAL_13_TM=60.427
PRIMER_LEFT_13_GC_PERCENT=52.632
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_INTERNAL_13_GC_PERCENT=54.545
PRIMER_INTERNAL_13_SELF_ANY_TH=7.82
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=0.00
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=0.00
PRIMER_RIGHT_13_HAIRPIN_TH=37.44
PRIMER_INTERNAL_13_HAIRPIN_TH=36.42
PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_13_END_STABILITY=4.7000
PRIMER_RIGHT_13_END_STABILITY=2.4300
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_14_PENALTY=2.402628
PRIMER_RIGHT_14_PENALTY=2.402628
PRIMER_INTERNAL_14_PENALTY=2.610811
PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT
PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_14=62,21
PRIMER_RIGHT_14=82,21
PRIMER_INTERNAL_14=125,22
PRIMER_LEFT_14_TM=57.597
PRIMER_RIGHT_14_TM=57.597
PRIMER_INTERNAL_14_TM=60.611
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_INTERNAL_14_GC_PERCENT=59.091
PRIMER_INTERNAL_14_SELF_ANY_TH=7.82
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=0.00
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=0.00
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=36.42
PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=2.2900
PRIMER_RIGHT_14_END_STABILITY=2.5200
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=30.4432
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_15_PENALTY=2.402628
PRIMER_RIGHT_15_PENALTY=2.402628
PRIMER_INTERNAL_15_PENALTY=2.740095
PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT
PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT
PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT
PRIMER_LEFT_15=60,21
PRIMER_RIGHT_15=80,21
PRIMER_INTERNAL_15=127,19
PRIMER_LEFT_15_TM=57.597
PRIMER_RIGHT_15_TM=57.597
PRIMER_INTERNAL_15_TM=58.260
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_INTERNAL_15_GC_PERCENT=63.158
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=0.00
PRIMER_RIGHT_15_HAIRPIN_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=36.42
PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=3.5500
PRIMER_RIGHT_15_END_STABILITY=1.9800
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0593
PRIMER_LEFT_16_PENALTY=2.455619
PRIMER_RIGHT_16_PENALTY=2.455619
PRIMER_INTERNAL_16_PENALTY=2.880381
PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG
PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC
PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_16=63,21
PRIMER_RIGHT_16=83,21
PRIMER_INTERNAL_16=120,22
PRIMER_LEFT_16_TM=57.544
PRIMER_RIGHT_16_TM=57.544
PRIMER_INTERNAL_16_TM=59.120
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_INTERNAL_16_GC_PERCENT=54.545
PRIMER_INTERNAL_16_SELF_ANY_TH=0.00
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=0.00
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=0.00
PRIMER_RIGHT_16_HAIRPIN_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=0.00
PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_LEFT_16_END_STABILITY=2.3900
PRIMER_RIGHT_16_END_STABILITY=2.7800
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=25.0463
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8601
PRIMER_LEFT_17_PENALTY=2.539776
PRIMER_RIGHT_17_PENALTY=2.539776
PRIMER_INTERNAL_17_PENALTY=2.939429
PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC
PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_17=122,20
PRIMER_RIGHT_17=141,20
PRIMER_INTERNAL_17=122,21
PRIMER_LEFT_17_TM=61.540
PRIMER_RIGHT_17_TM=61.540
PRIMER_INTERNAL_17_TM=58.061
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_TH=37.44
PRIMER_INTERNAL_17_HAIRPIN_TH=0.00
PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_17_END_STABILITY=4.7000
PRIMER_RIGHT_17_END_STABILITY=2.4400
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_18_PENALTY=2.539776
PRIMER_RIGHT_18_PENALTY=2.539776
PRIMER_INTERNAL_18_PENALTY=2.939429
PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_18=121,20
PRIMER_RIGHT_18=140,20
PRIMER_INTERNAL_18=121,21
PRIMER_LEFT_18_TM=61.540
PRIMER_RIGHT_18_TM=61.540
PRIMER_INTERNAL_18_TM=58.061
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_INTERNAL_18_GC_PERCENT=57.143
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_TH=37.44
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_18_END_STABILITY=5.0300
PRIMER_RIGHT_18_END_STABILITY=3.2800
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_19_PENALTY=2.587613
PRIMER_RIGHT_19_PENALTY=2.587613
PRIMER_INTERNAL_19_PENALTY=3.476553
PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC
PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_19=120,19
PRIMER_RIGHT_19=138,19
PRIMER_INTERNAL_19=123,23
PRIMER_LEFT_19_TM=60.588
PRIMER_RIGHT_19_TM=60.588
PRIMER_INTERNAL_19_TM=60.477
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_INTERNAL_19_GC_PERCENT=52.174
PRIMER_INTERNAL_19_SELF_ANY_TH=7.82
PRIMER_LEFT_19_SELF_ANY_TH=0.00
PRIMER_RIGHT_19_SELF_ANY_TH=0.00
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=36.42
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_19_END_STABILITY=5.3400
PRIMER_RIGHT_19_END_STABILITY=3.5300
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=2.588667
PRIMER_RIGHT_20_PENALTY=2.588667
PRIMER_INTERNAL_20_PENALTY=3.598972
PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT
PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_20=121,19
PRIMER_RIGHT_20=139,19
PRIMER_INTERNAL_20=119,23
PRIMER_LEFT_20_TM=60.589
PRIMER_RIGHT_20_TM=60.589
PRIMER_INTERNAL_20_TM=59.401
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=52.632
PRIMER_INTERNAL_20_GC_PERCENT=52.174
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=0.00
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=0.00
PRIMER_LEFT_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_TH=37.44
PRIMER_INTERNAL_20_HAIRPIN_TH=0.00
PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_20_END_STABILITY=5.0700
PRIMER_RIGHT_20_END_STABILITY=3.2800
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_21_PENALTY=2.627395
PRIMER_RIGHT_21_PENALTY=2.627395
PRIMER_INTERNAL_21_PENALTY=3.606233
PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA
PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT
PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG
PRIMER_LEFT_21=52,21
PRIMER_RIGHT_21=72,21
PRIMER_INTERNAL_21=126,18
PRIMER_LEFT_21_TM=57.373
PRIMER_RIGHT_21_TM=57.373
PRIMER_INTERNAL_21_TM=58.394
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_INTERNAL_21_GC_PERCENT=66.667
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.14
PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_21_END_STABILITY=4.5200
PRIMER_RIGHT_21_END_STABILITY=3.0600
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=1.1837
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_22_PENALTY=2.678056
PRIMER_RIGHT_22_PENALTY=2.678056
PRIMER_INTERNAL_22_PENALTY=3.829005
PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT
PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA
PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_22=58,21
PRIMER_RIGHT_22=78,21
PRIMER_INTERNAL_22=124,23
PRIMER_LEFT_22_TM=57.322
PRIMER_RIGHT_22_TM=57.322
PRIMER_INTERNAL_22_TM=60.829
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_INTERNAL_22_GC_PERCENT=56.522
PRIMER_INTERNAL_22_SELF_ANY_TH=7.82
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=0.00
PRIMER_RIGHT_22_HAIRPIN_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=36.42
PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_22_END_STABILITY=3.4100
PRIMER_RIGHT_22_END_STABILITY=2.1500
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=25.2513
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=19.0855
PRIMER_LEFT_23_PENALTY=2.780624
PRIMER_RIGHT_23_PENALTY=2.780624
PRIMER_INTERNAL_23_PENALTY=3.865064
PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT
PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_23=62,19
PRIMER_RIGHT_23=80,19
PRIMER_INTERNAL_23=120,21
PRIMER_LEFT_23_TM=57.219
PRIMER_RIGHT_23_TM=57.219
PRIMER_INTERNAL_23_TM=57.135
PRIMER_LEFT_23_GC_PERCENT=47.368
PRIMER_RIGHT_23_GC_PERCENT=47.368
PRIMER_INTERNAL_23_GC_PERCENT=52.381
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=0.00
PRIMER_RIGHT_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_23_END_STABILITY=3.5500
PRIMER_RIGHT_23_END_STABILITY=2.5200
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_24_PENALTY=2.999071
PRIMER_RIGHT_24_PENALTY=2.999071
PRIMER_INTERNAL_24_PENALTY=3.905453
PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA
PRIMER_LEFT_24=120,20
PRIMER_RIGHT_24=139,20
PRIMER_INTERNAL_24=127,18
PRIMER_LEFT_24_TM=61.999
PRIMER_RIGHT_24_TM=61.999
PRIMER_INTERNAL_24_TM=58.095
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_INTERNAL_24_GC_PERCENT=66.667
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=0.00
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=0.00
PRIMER_RIGHT_24_HAIRPIN_TH=37.44
PRIMER_INTERNAL_24_HAIRPIN_TH=36.42
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_24_END_STABILITY=5.0700
PRIMER_RIGHT_24_END_STABILITY=3.5300
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_25_PENALTY=2.999229
PRIMER_RIGHT_25_PENALTY=2.999229
PRIMER_INTERNAL_25_PENALTY=3.954555
PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG
PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC
PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_25=59,21
PRIMER_RIGHT_25=79,21
PRIMER_INTERNAL_25=120,23
PRIMER_LEFT_25_TM=57.001
PRIMER_RIGHT_25_TM=57.001
PRIMER_INTERNAL_25_TM=60.955
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_INTERNAL_25_GC_PERCENT=56.522
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=0.00
PRIMER_RIGHT_25_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=0.00
PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_25_END_STABILITY=3.6600
PRIMER_RIGHT_25_END_STABILITY=2.0800
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=30.7683
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=20.4326
PRIMER_LEFT_26_PENALTY=2.999229
PRIMER_RIGHT_26_PENALTY=2.999229
PRIMER_INTERNAL_26_PENALTY=4.240075
PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC
PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG
PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_26=57,21
PRIMER_RIGHT_26=77,21
PRIMER_INTERNAL_26=122,23
PRIMER_LEFT_26_TM=57.001
PRIMER_RIGHT_26_TM=57.001
PRIMER_INTERNAL_26_TM=61.240
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=56.522
PRIMER_INTERNAL_26_SELF_ANY_TH=7.82
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=0.00
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=0.00
PRIMER_LEFT_26_HAIRPIN_TH=0.00
PRIMER_RIGHT_26_HAIRPIN_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=36.42
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=3.5100
PRIMER_RIGHT_26_END_STABILITY=2.9000
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=20.2809
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.6247
PRIMER_LEFT_27_PENALTY=3.455227
PRIMER_RIGHT_27_PENALTY=3.455227
PRIMER_INTERNAL_27_PENALTY=4.486709
PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_27=118,21
PRIMER_RIGHT_27=138,21
PRIMER_INTERNAL_27=119,22
PRIMER_LEFT_27_TM=61.455
PRIMER_RIGHT_27_TM=61.455
PRIMER_INTERNAL_27_TM=57.513
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=50.000
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=0.00
PRIMER_RIGHT_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=0.00
PRIMER_RIGHT_27_SELF_END_TH=0.00
PRIMER_LEFT_27_HAIRPIN_TH=0.00
PRIMER_RIGHT_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=5.3400
PRIMER_RIGHT_27_END_STABILITY=3.0100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_28_PENALTY=3.677573
PRIMER_RIGHT_28_PENALTY=3.677573
PRIMER_INTERNAL_28_PENALTY=4.513970
PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC
PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_28=123,20
PRIMER_RIGHT_28=142,20
PRIMER_INTERNAL_28=118,24
PRIMER_LEFT_28_TM=62.678
PRIMER_RIGHT_28_TM=62.678
PRIMER_INTERNAL_28_TM=59.486
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_INTERNAL_28_GC_PERCENT=50.000
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=37.44
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_28_END_STABILITY=4.3000
PRIMER_RIGHT_28_END_STABILITY=2.4300
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.1346
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_29_PENALTY=4.100862
PRIMER_RIGHT_29_PENALTY=4.100862
PRIMER_INTERNAL_29_PENALTY=5.029817
PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC
PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC
PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_29=128,19
PRIMER_RIGHT_29=146,19
PRIMER_INTERNAL_29=123,24
PRIMER_LEFT_29_TM=62.101
PRIMER_RIGHT_29_TM=62.101
PRIMER_INTERNAL_29_TM=61.030
PRIMER_LEFT_29_GC_PERCENT=63.158
PRIMER_RIGHT_29_GC_PERCENT=63.158
PRIMER_INTERNAL_29_GC_PERCENT=54.167
PRIMER_INTERNAL_29_SELF_ANY_TH=7.82
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=38.29
PRIMER_RIGHT_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=36.42
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_29_END_STABILITY=3.6900
PRIMER_RIGHT_29_END_STABILITY=3.6800
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0362
PRIMER_LEFT_30_PENALTY=4.194272
PRIMER_RIGHT_30_PENALTY=4.194272
PRIMER_INTERNAL_30_PENALTY=5.052238
PRIMER_LEFT_30_SEQUENCE=AAACGCAAAGCACGCTCCC
PRIMER_RIGHT_30_SEQUENCE=GGGAGCGTGCTTTGCGTTT
PRIMER_INTERNAL_30_SEQUENCE=CCAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_30=124,19
PRIMER_RIGHT_30=142,19
PRIMER_INTERNAL_30=121,23
PRIMER_LEFT_30_TM=62.194
PRIMER_RIGHT_30_TM=62.194
PRIMER_INTERNAL_30_TM=62.052
PRIMER_LEFT_30_GC_PERCENT=57.895
PRIMER_RIGHT_30_GC_PERCENT=57.895
PRIMER_INTERNAL_30_GC_PERCENT=60.870
PRIMER_INTERNAL_30_SELF_ANY_TH=0.00
PRIMER_LEFT_30_SELF_ANY_TH=0.00
PRIMER_RIGHT_30_SELF_ANY_TH=0.00
PRIMER_INTERNAL_30_SELF_END_TH=0.00
PRIMER_LEFT_30_SELF_END_TH=0.00
PRIMER_RIGHT_30_SELF_END_TH=0.00
PRIMER_LEFT_30_HAIRPIN_TH=0.00
PRIMER_RIGHT_30_HAIRPIN_TH=37.44
PRIMER_INTERNAL_30_HAIRPIN_TH=41.14
PRIMER_LEFT_30_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_30_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_30_END_STABILITY=4.3000
PRIMER_RIGHT_30_END_STABILITY=3.6000
PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=9.8734
PRIMER_LEFT_31_PENALTY=4.473143
PRIMER_RIGHT_31_PENALTY=4.473143
PRIMER_INTERNAL_31_PENALTY=5.152054
PRIMER_LEFT_31_SEQUENCE=TTCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_31_SEQUENCE=AGCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_31_SEQUENCE=TTCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_31=119,21
PRIMER_RIGHT_31=139,21
PRIMER_INTERNAL_31=119,24
PRIMER_LEFT_31_TM=62.473
PRIMER_RIGHT_31_TM=62.473
PRIMER_INTERNAL_31_TM=61.152
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_INTERNAL_31_GC_PERCENT=54.167
PRIMER_INTERNAL_31_SELF_ANY_TH=0.00
PRIMER_LEFT_31_SELF_ANY_TH=0.00
PRIMER_RIGHT_31_SELF_ANY_TH=0.00
PRIMER_INTERNAL_31_SELF_END_TH=0.00
PRIMER_LEFT_31_SELF_END_TH=0.00
PRIMER_RIGHT_31_SELF_END_TH=0.00
PRIMER_LEFT_31_HAIRPIN_TH=0.00
PRIMER_RIGHT_31_HAIRPIN_TH=37.44
PRIMER_INTERNAL_31_HAIRPIN_TH=0.00
PRIMER_LEFT_31_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_31_END_STABILITY=5.0700
PRIMER_RIGHT_31_END_STABILITY=3.5300
PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_32_PENALTY=4.837196
PRIMER_RIGHT_32_PENALTY=4.837196
PRIMER_INTERNAL_32_PENALTY=5.256814
PRIMER_LEFT_32_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_32_SEQUENCE=GAGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_32_SEQUENCE=CAAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_32=120,21
PRIMER_RIGHT_32=140,21
PRIMER_INTERNAL_32=122,24
PRIMER_LEFT_32_TM=62.837
PRIMER_RIGHT_32_TM=62.837
PRIMER_INTERNAL_32_TM=61.257
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_INTERNAL_32_GC_PERCENT=54.167
PRIMER_INTERNAL_32_SELF_ANY_TH=7.82
PRIMER_LEFT_32_SELF_ANY_TH=0.00
PRIMER_RIGHT_32_SELF_ANY_TH=0.00
PRIMER_INTERNAL_32_SELF_END_TH=0.00
PRIMER_LEFT_32_SELF_END_TH=0.00
PRIMER_RIGHT_32_SELF_END_TH=0.00
PRIMER_LEFT_32_HAIRPIN_TH=0.00
PRIMER_RIGHT_32_HAIRPIN_TH=37.44
PRIMER_INTERNAL_32_HAIRPIN_TH=36.42
PRIMER_LEFT_32_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_32_END_STABILITY=5.0300
PRIMER_RIGHT_32_END_STABILITY=3.5300
PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_INTERNAL_33_PENALTY=5.325970
PRIMER_INTERNAL_33_SEQUENCE=ATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_33=118,23
PRIMER_INTERNAL_33_TM=57.674
PRIMER_INTERNAL_33_GC_PERCENT=47.826
PRIMER_INTERNAL_33_SELF_ANY_TH=0.00
PRIMER_INTERNAL_33_SELF_END_TH=0.00
PRIMER_INTERNAL_33_HAIRPIN_TH=0.00
PRIMER_INTERNAL_34_PENALTY=5.931698
PRIMER_INTERNAL_34_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_34=117,25
PRIMER_INTERNAL_34_TM=59.068
PRIMER_INTERNAL_34_GC_PERCENT=48.000
PRIMER_INTERNAL_34_SELF_ANY_TH=0.00
PRIMER_INTERNAL_34_SELF_END_TH=0.00
PRIMER_INTERNAL_34_HAIRPIN_TH=0.00
PRIMER_INTERNAL_35_PENALTY=6.171494
PRIMER_INTERNAL_35_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_35=118,25
PRIMER_INTERNAL_35_TM=61.171
PRIMER_INTERNAL_35_GC_PERCENT=52.000
PRIMER_INTERNAL_35_SELF_ANY_TH=0.00
PRIMER_INTERNAL_35_SELF_END_TH=0.00
PRIMER_INTERNAL_35_HAIRPIN_TH=0.00
PRIMER_INTERNAL_36_PENALTY=6.354311
PRIMER_INTERNAL_36_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_36=116,26
PRIMER_INTERNAL_36_TM=59.646
PRIMER_INTERNAL_36_GC_PERCENT=50.000
PRIMER_INTERNAL_36_SELF_ANY_TH=0.00
PRIMER_INTERNAL_36_SELF_END_TH=0.00
PRIMER_INTERNAL_36_HAIRPIN_TH=0.00
PRIMER_INTERNAL_37_PENALTY=6.689016
PRIMER_INTERNAL_37_SEQUENCE=TATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_37=117,24
PRIMER_INTERNAL_37_TM=57.311
PRIMER_INTERNAL_37_GC_PERCENT=45.833
PRIMER_INTERNAL_37_SELF_ANY_TH=0.00
PRIMER_INTERNAL_37_SELF_END_TH=0.00
PRIMER_INTERNAL_37_HAIRPIN_TH=0.00
PRIMER_INTERNAL_38_PENALTY=6.689016
PRIMER_INTERNAL_38_SEQUENCE=TCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_38=115,24
PRIMER_INTERNAL_38_TM=57.311
PRIMER_INTERNAL_38_GC_PERCENT=45.833
PRIMER_INTERNAL_38_SELF_ANY_TH=0.00
PRIMER_INTERNAL_38_SELF_END_TH=0.00
PRIMER_INTERNAL_38_HAIRPIN_TH=0.00
PRIMER_INTERNAL_39_PENALTY=6.694952
PRIMER_INTERNAL_39_SEQUENCE=CTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_39=116,24
PRIMER_INTERNAL_39_TM=57.305
PRIMER_INTERNAL_39_GC_PERCENT=45.833
PRIMER_INTERNAL_39_SELF_ANY_TH=0.00
PRIMER_INTERNAL_39_SELF_END_TH=0.00
PRIMER_INTERNAL_39_HAIRPIN_TH=0.00
PRIMER_INTERNAL_40_PENALTY=6.707310
PRIMER_INTERNAL_40_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_40=117,26
PRIMER_INTERNAL_40_TM=60.707
PRIMER_INTERNAL_40_GC_PERCENT=50.000
PRIMER_INTERNAL_40_SELF_ANY_TH=0.00
PRIMER_INTERNAL_40_SELF_END_TH=0.00
PRIMER_INTERNAL_40_HAIRPIN_TH=0.00
PRIMER_INTERNAL_41_PENALTY=6.724062
PRIMER_INTERNAL_41_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_41=115,25
PRIMER_INTERNAL_41_TM=58.276
PRIMER_INTERNAL_41_GC_PERCENT=44.000
PRIMER_INTERNAL_41_SELF_ANY_TH=0.00
PRIMER_INTERNAL_41_SELF_END_TH=0.00
PRIMER_INTERNAL_41_HAIRPIN_TH=0.00
PRIMER_INTERNAL_42_PENALTY=6.744217
PRIMER_INTERNAL_42_SEQUENCE=CAAAACGCAAAGCACGCTCCCGATC
PRIMER_INTERNAL_42=122,25
PRIMER_INTERNAL_42_TM=61.744
PRIMER_INTERNAL_42_GC_PERCENT=56.000
PRIMER_INTERNAL_42_SELF_ANY_TH=7.82
PRIMER_INTERNAL_42_SELF_END_TH=0.00
PRIMER_INTERNAL_42_HAIRPIN_TH=36.42
PRIMER_INTERNAL_43_PENALTY=6.785590
PRIMER_INTERNAL_43_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_43=114,25
PRIMER_INTERNAL_43_TM=58.214
PRIMER_INTERNAL_43_GC_PERCENT=48.000
PRIMER_INTERNAL_43_SELF_ANY_TH=0.00
PRIMER_INTERNAL_43_SELF_END_TH=0.00
PRIMER_INTERNAL_43_HAIRPIN_TH=0.00
PRIMER_INTERNAL_44_PENALTY=6.889273
PRIMER_INTERNAL_44_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_44=114,26
PRIMER_INTERNAL_44_TM=59.111
PRIMER_INTERNAL_44_GC_PERCENT=46.154
PRIMER_INTERNAL_44_SELF_ANY_TH=0.00
PRIMER_INTERNAL_44_SELF_END_TH=0.00
PRIMER_INTERNAL_44_HAIRPIN_TH=0.00
PRIMER_INTERNAL_45_PENALTY=6.889273
PRIMER_INTERNAL_45_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_45=113,26
PRIMER_INTERNAL_45_TM=59.111
PRIMER_INTERNAL_45_GC_PERCENT=46.154
PRIMER_INTERNAL_45_SELF_ANY_TH=0.00
PRIMER_INTERNAL_45_SELF_END_TH=0.00
PRIMER_INTERNAL_45_HAIRPIN_TH=0.00
PRIMER_INTERNAL_46_PENALTY=6.910113
PRIMER_INTERNAL_46_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGA
PRIMER_INTERNAL_46=121,24
PRIMER_INTERNAL_46_TM=62.910
PRIMER_INTERNAL_46_GC_PERCENT=58.333
PRIMER_INTERNAL_46_SELF_ANY_TH=7.82
PRIMER_INTERNAL_46_SELF_END_TH=0.00
PRIMER_INTERNAL_46_HAIRPIN_TH=36.42
PRIMER_INTERNAL_47_PENALTY=6.910113
PRIMER_INTERNAL_47_SEQUENCE=TCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_47=120,24
PRIMER_INTERNAL_47_TM=62.910
PRIMER_INTERNAL_47_GC_PERCENT=58.333
PRIMER_INTERNAL_47_SELF_ANY_TH=0.00
PRIMER_INTERNAL_47_SELF_END_TH=0.00
PRIMER_INTERNAL_47_HAIRPIN_TH=41.14
PRIMER_INTERNAL_48_PENALTY=7.008353
PRIMER_INTERNAL_48_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_48=116,25
PRIMER_INTERNAL_48_TM=57.992
PRIMER_INTERNAL_48_GC_PERCENT=48.000
PRIMER_INTERNAL_48_SELF_ANY_TH=0.00
PRIMER_INTERNAL_48_SELF_END_TH=0.00
PRIMER_INTERNAL_48_HAIRPIN_TH=0.00
PRIMER_INTERNAL_49_PENALTY=7.035358
PRIMER_INTERNAL_49_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGCT
PRIMER_INTERNAL_49=113,27
PRIMER_INTERNAL_49_TM=59.965
PRIMER_INTERNAL_49_GC_PERCENT=44.444
PRIMER_INTERNAL_49_SELF_ANY_TH=0.00
PRIMER_INTERNAL_49_SELF_END_TH=0.00
PRIMER_INTERNAL_49_HAIRPIN_TH=0.00
PRIMER_INTERNAL_50_PENALTY=7.102160
PRIMER_INTERNAL_50_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_50=115,26
PRIMER_INTERNAL_50_TM=58.898
PRIMER_INTERNAL_50_GC_PERCENT=46.154
PRIMER_INTERNAL_50_SELF_ANY_TH=0.00
PRIMER_INTERNAL_50_SELF_END_TH=0.00
PRIMER_INTERNAL_50_HAIRPIN_TH=0.00
PRIMER_INTERNAL_51_PENALTY=7.334450
PRIMER_INTERNAL_51_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCTC
PRIMER_INTERNAL_51=114,27
PRIMER_INTERNAL_51_TM=59.666
PRIMER_INTERNAL_51_GC_PERCENT=48.148
PRIMER_INTERNAL_51_SELF_ANY_TH=0.00
PRIMER_INTERNAL_51_SELF_END_TH=0.00
PRIMER_INTERNAL_51_HAIRPIN_TH=0.00
PRIMER_INTERNAL_52_PENALTY=7.480679
PRIMER_INTERNAL_52_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTCC
PRIMER_INTERNAL_52=115,27
PRIMER_INTERNAL_52_TM=60.481
PRIMER_INTERNAL_52_GC_PERCENT=48.148
PRIMER_INTERNAL_52_SELF_ANY_TH=0.00
PRIMER_INTERNAL_52_SELF_END_TH=0.00
PRIMER_INTERNAL_52_HAIRPIN_TH=0.00
PRIMER_INTERNAL_53_PENALTY=7.647244
PRIMER_INTERNAL_53_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACGC
PRIMER_INTERNAL_53=112,27
PRIMER_INTERNAL_53_TM=59.353
PRIMER_INTERNAL_53_GC_PERCENT=44.444
PRIMER_INTERNAL_53_SELF_ANY_TH=0.00
PRIMER_INTERNAL_53_SELF_END_TH=0.00
PRIMER_INTERNAL_53_HAIRPIN_TH=0.00
PRIMER_INTERNAL_54_PENALTY=7.744113
PRIMER_INTERNAL_54_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_54=55,26
PRIMER_INTERNAL_54_TM=58.256
PRIMER_INTERNAL_54_GC_PERCENT=46.154
PRIMER_INTERNAL_54_SELF_ANY_TH=0.00
PRIMER_INTERNAL_54_SELF_END_TH=0.00
PRIMER_INTERNAL_54_HAIRPIN_TH=0.00
PRIMER_INTERNAL_55_PENALTY=7.867066
PRIMER_INTERNAL_55_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGAT
PRIMER_INTERNAL_55=121,25
PRIMER_INTERNAL_55_TM=62.867
PRIMER_INTERNAL_55_GC_PERCENT=56.000
PRIMER_INTERNAL_55_SELF_ANY_TH=7.82
PRIMER_INTERNAL_55_SELF_END_TH=0.00
PRIMER_INTERNAL_55_HAIRPIN_TH=36.42
PRIMER_INTERNAL_56_PENALTY=7.916609
PRIMER_INTERNAL_56_SEQUENCE=TGACCTGATAGAAACGCCAACTCAC
PRIMER_INTERNAL_56=53,25
PRIMER_INTERNAL_56_TM=57.083
PRIMER_INTERNAL_56_GC_PERCENT=48.000
PRIMER_INTERNAL_56_SELF_ANY_TH=0.00
PRIMER_INTERNAL_56_SELF_END_TH=0.00
PRIMER_INTERNAL_56_HAIRPIN_TH=0.00
PRIMER_INTERNAL_57_PENALTY=7.922893
PRIMER_INTERNAL_57_SEQUENCE=CCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_57=56,25
PRIMER_INTERNAL_57_TM=57.077
PRIMER_INTERNAL_57_GC_PERCENT=48.000
PRIMER_INTERNAL_57_SELF_ANY_TH=0.00
PRIMER_INTERNAL_57_SELF_END_TH=0.00
PRIMER_INTERNAL_57_HAIRPIN_TH=0.00
PRIMER_INTERNAL_58_PENALTY=7.922893
PRIMER_INTERNAL_58_SEQUENCE=ACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_58=55,25
PRIMER_INTERNAL_58_TM=57.077
PRIMER_INTERNAL_58_GC_PERCENT=48.000
PRIMER_INTERNAL_58_SELF_ANY_TH=0.00
PRIMER_INTERNAL_58_SELF_END_TH=0.00
PRIMER_INTERNAL_58_HAIRPIN_TH=0.00
PRIMER_INTERNAL_59_PENALTY=7.963894
PRIMER_INTERNAL_59_SEQUENCE=TGACCTGATAGAAACGCCAACTCACT
PRIMER_INTERNAL_59=53,26
PRIMER_INTERNAL_59_TM=58.036
PRIMER_INTERNAL_59_GC_PERCENT=46.154
PRIMER_INTERNAL_59_SELF_ANY_TH=0.00
PRIMER_INTERNAL_59_SELF_END_TH=0.00
PRIMER_INTERNAL_59_HAIRPIN_TH=0.00
PRIMER_INTERNAL_60_PENALTY=8.131843
PRIMER_INTERNAL_60_SEQUENCE=TGACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_60=53,27
PRIMER_INTERNAL_60_TM=58.868
PRIMER_INTERNAL_60_GC_PERCENT=48.148
PRIMER_INTERNAL_60_SELF_ANY_TH=0.00
PRIMER_INTERNAL_60_SELF_END_TH=0.00
PRIMER_INTERNAL_60_HAIRPIN_TH=0.00
PRIMER_INTERNAL_61_PENALTY=8.132460
PRIMER_INTERNAL_61_SEQUENCE=GACCTGATAGAAACGCCAACTCACTGT
PRIMER_INTERNAL_61=54,27
PRIMER_INTERNAL_61_TM=58.868
PRIMER_INTERNAL_61_GC_PERCENT=48.148
PRIMER_INTERNAL_61_SELF_ANY_TH=0.00
PRIMER_INTERNAL_61_SELF_END_TH=0.00
PRIMER_INTERNAL_61_HAIRPIN_TH=0.00
PRIMER_INTERNAL_62_PENALTY=8.193320
PRIMER_INTERNAL_62_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCCC
PRIMER_INTERNAL_62=116,27
PRIMER_INTERNAL_62_TM=61.193
PRIMER_INTERNAL_62_GC_PERCENT=51.852
PRIMER_INTERNAL_62_SELF_ANY_TH=0.00
PRIMER_INTERNAL_62_SELF_END_TH=0.00
PRIMER_INTERNAL_62_HAIRPIN_TH=0.00
PRIMER_INTERNAL_63_PENALTY=8.237701
PRIMER_INTERNAL_63_SEQUENCE=GACCTGATAGAAACGCCAACTCACTG
PRIMER_INTERNAL_63=54,26
PRIMER_INTERNAL_63_TM=57.762
PRIMER_INTERNAL_63_GC_PERCENT=50.000
PRIMER_INTERNAL_63_SELF_ANY_TH=0.00
PRIMER_INTERNAL_63_SELF_END_TH=0.00
PRIMER_INTERNAL_63_HAIRPIN_TH=0.00
PRIMER_INTERNAL_64_PENALTY=8.755267
PRIMER_INTERNAL_64_SEQUENCE=ATGACCTGATAGAAACGCCAACTCAC
PRIMER_INTERNAL_64=52,26
PRIMER_INTERNAL_64_TM=57.245
PRIMER_INTERNAL_64_GC_PERCENT=46.154
PRIMER_INTERNAL_64_SELF_ANY_TH=0.00
PRIMER_INTERNAL_64_SELF_END_TH=0.00
PRIMER_INTERNAL_64_HAIRPIN_TH=0.00
PRIMER_INTERNAL_65_PENALTY=8.758760
PRIMER_INTERNAL_65_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACG
PRIMER_INTERNAL_65=112,26
PRIMER_INTERNAL_65_TM=57.241
PRIMER_INTERNAL_65_GC_PERCENT=42.308
PRIMER_INTERNAL_65_SELF_ANY_TH=0.00
PRIMER_INTERNAL_65_SELF_END_TH=0.00
PRIMER_INTERNAL_65_HAIRPIN_TH=0.00
PRIMER_INTERNAL_66_PENALTY=8.838967
PRIMER_INTERNAL_66_SEQUENCE=ATGACCTGATAGAAACGCCAACTCACT
PRIMER_INTERNAL_66=52,27
PRIMER_INTERNAL_66_TM=58.161
PRIMER_INTERNAL_66_GC_PERCENT=44.444
PRIMER_INTERNAL_66_SELF_ANY_TH=0.00
PRIMER_INTERNAL_66_SELF_END_TH=0.00
PRIMER_INTERNAL_66_HAIRPIN_TH=0.00
PRIMER_INTERNAL_67_PENALTY=8.978186
PRIMER_INTERNAL_67_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_67=118,26
PRIMER_INTERNAL_67_TM=62.978
PRIMER_INTERNAL_67_GC_PERCENT=53.846
PRIMER_INTERNAL_67_SELF_ANY_TH=0.00
PRIMER_INTERNAL_67_SELF_END_TH=0.00
PRIMER_INTERNAL_67_HAIRPIN_TH=41.14
PRIMER_INTERNAL_68_PENALTY=9.099564
PRIMER_INTERNAL_68_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGTA
PRIMER_INTERNAL_68=55,27
PRIMER_INTERNAL_68_TM=57.900
PRIMER_INTERNAL_68_GC_PERCENT=44.444
PRIMER_INTERNAL_68_SELF_ANY_TH=0.00
PRIMER_INTERNAL_68_SELF_END_TH=0.00
PRIMER_INTERNAL_68_HAIRPIN_TH=0.00
PRIMER_INTERNAL_69_PENALTY=9.448747
PRIMER_INTERNAL_69_SEQUENCE=AAAGTCTATTCCAAAACGCAAAGCACG
PRIMER_INTERNAL_69=111,27
PRIMER_INTERNAL_69_TM=57.551
PRIMER_INTERNAL_69_GC_PERCENT=40.741
PRIMER_INTERNAL_69_SELF_ANY_TH=0.00
PRIMER_INTERNAL_69_SELF_END_TH=0.00
PRIMER_INTERNAL_69_HAIRPIN_TH=0.00
PRIMER_INTERNAL_70_PENALTY=9.472089
PRIMER_INTERNAL_70_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCCG
PRIMER_INTERNAL_70=117,27
PRIMER_INTERNAL_70_TM=62.472
PRIMER_INTERNAL_70_GC_PERCENT=51.852
PRIMER_INTERNAL_70_SELF_ANY_TH=0.00
PRIMER_INTERNAL_70_SELF_END_TH=0.00
PRIMER_INTERNAL_70_HAIRPIN_TH=41.14
PRIMER_INTERNAL_71_PENALTY=9.912698
PRIMER_INTERNAL_71_SEQUENCE=CGTTGTCAAAGTCTATTCCAAAACGCA
PRIMER_INTERNAL_71=104,27
PRIMER_INTERNAL_71_TM=57.087
PRIMER_INTERNAL_71_GC_PERCENT=40.741
PRIMER_INTERNAL_71_SELF_ANY_TH=0.00
PRIMER_INTERNAL_71_SELF_END_TH=0.00
PRIMER_INTERNAL_71_HAIRPIN_TH=26.69
PRIMER_INTERNAL_72_PENALTY=9.914131
PRIMER_INTERNAL_72_SEQUENCE=ACGTTGTCAAAGTCTATTCCAAAACGC
PRIMER_INTERNAL_72=103,27
PRIMER_INTERNAL_72_TM=57.086
PRIMER_INTERNAL_72_GC_PERCENT=40.741
PRIMER_INTERNAL_72_SELF_ANY_TH=0.00
PRIMER_INTERNAL_72_SELF_END_TH=0.00
PRIMER_INTERNAL_72_HAIRPIN_TH=35.24
=
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_NUM_RETURN=1
P3_FILE_FLAG=0
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_sequencing_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_ID=pick_sequencing_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_WARNING=No left primer found in range 1 - 40
PRIMER_LEFT_EXPLAIN=sequencing locations 1, considered 56, too many Ns 5, low tm 51, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
PRIMER_PICK_RIGHT_PRIMER=1
SEQUENCE_ID=pick_sequencing_primers_th_3
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_WARNING=No left primer found in range 1 - 40
PRIMER_LEFT_EXPLAIN=sequencing locations 1, considered 56, too many Ns 5, low tm 51, ok 0
PRIMER_RIGHT_EXPLAIN=sequencing locations 1, considered 63, low tm 33, high tm 13, ok 1
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_RIGHT_0=137,20
PRIMER_RIGHT_0_TM=58.514
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
=
PRIMER_TASK=check_primers
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=check_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
SEQUENCE_PRIMER=GACATGACACTGACGATGCA
SEQUENCE_PRIMER_REVCOMP=TAGCTATCGATTTGGGTCGG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, low tm 1, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=check_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_PRIMER=TGACCTGATAGAAACGCCAA
SEQUENCE_PRIMER_REVCOMP=TGCTTTGCGTTTTGGAATAG
PRIMER_LEFT_EXPLAIN=considered 1, low tm 1, ok 0
PRIMER_RIGHT_EXPLAIN=considered 1, low tm 1, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_TASK=pick_discriminative_primers
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_disc_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 3, low tm 2, ok 3
PRIMER_RIGHT_EXPLAIN=considered 3, low tm 3, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_disc_primer_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 3, low tm 3, ok 3
PRIMER_RIGHT_EXPLAIN=considered 3, low tm 2, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_TASK=pick_cloning_primers
PRIMER_PRODUCT_SIZE_RANGE=200-300
SEQUENCE_ID=pick_disc_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=2,100
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
PRIMER_PRODUCT_SIZE_RANGE=200-300
SEQUENCE_ID=pick_disc_primer_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_TARGET=2,120
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
