SEQUENCE_ID=io_compl
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
PRIMER_PAIR_WT_IO_PENALTY=1
PRIMER_PAIR_WT_PR_PENALTY=0
PRIMER_WT_TM_GT=0
PRIMER_WT_TM_LT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_SIZE_GT=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_WT_TM_GT=0
PRIMER_INTERNAL_WT_TM_LT=0
PRIMER_INTERNAL_WT_SIZE_LT=0
PRIMER_INTERNAL_WT_SIZE_GT=0
PRIMER_INTERNAL_WT_SELF_ANY=1
PRIMER_INTERNAL_WT_SELF_END=2
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, high any compl 1, high end compl 72, ok 108
PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, high any compl 1, high end compl 47, ok 76
PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 32, low tm 3, high tm 886, ok 644
PRIMER_PAIR_EXPLAIN=considered 10429, unacceptable product size 5893, high end compl 435, no internal oligo 767, ok 3334
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_INTERNAL_0_PENALTY=1.000000
PRIMER_LEFT_0_SEQUENCE=AAGACTCCGCCAGCCTTG
PRIMER_RIGHT_0_SEQUENCE=CAGCCTGACGGTCATCAT
PRIMER_INTERNAL_0_SEQUENCE=CCTCTCCCTCAACTCACC
PRIMER_LEFT_0=56,18
PRIMER_RIGHT_0=118,18
PRIMER_INTERNAL_0=83,18
PRIMER_LEFT_0_TM=61.507
PRIMER_RIGHT_0_TM=58.043
PRIMER_INTERNAL_0_TM=55.425
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=55.556
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=63
PRIMER_PAIR_0_PRODUCT_TM=76.4
PRIMER_PAIR_1_PENALTY=1.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_INTERNAL_1_PENALTY=1.000000
PRIMER_LEFT_1_SEQUENCE=AAGACTCCGCCAGCCTTG
PRIMER_RIGHT_1_SEQUENCE=TCAGCCTGACGGTCATCAT
PRIMER_INTERNAL_1_SEQUENCE=CCTCTCCCTCAACTCACC
PRIMER_LEFT_1=56,18
PRIMER_RIGHT_1=119,19
PRIMER_INTERNAL_1=83,18
PRIMER_LEFT_1_TM=61.507
PRIMER_RIGHT_1_TM=60.222
PRIMER_INTERNAL_1_TM=55.425
PRIMER_LEFT_1_GC_PERCENT=61.111
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=64
PRIMER_PAIR_1_PRODUCT_TM=76.2
PRIMER_PAIR_2_PENALTY=1.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_INTERNAL_2_PENALTY=1.000000
PRIMER_LEFT_2_SEQUENCE=AAGACTCCGCCAGCCTTG
PRIMER_RIGHT_2_SEQUENCE=CTCAGCCTGACGGTCATC
PRIMER_INTERNAL_2_SEQUENCE=CTCTCCCTCAACTCACCA
PRIMER_LEFT_2=56,18
PRIMER_RIGHT_2=120,18
PRIMER_INTERNAL_2=84,18
PRIMER_LEFT_2_TM=61.507
PRIMER_RIGHT_2_TM=57.723
PRIMER_INTERNAL_2_TM=54.310
PRIMER_LEFT_2_GC_PERCENT=61.111
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_INTERNAL_2_GC_PERCENT=55.556
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=65
PRIMER_PAIR_2_PRODUCT_TM=76.5
PRIMER_PAIR_3_PENALTY=1.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_INTERNAL_3_PENALTY=1.000000
PRIMER_LEFT_3_SEQUENCE=AAGACTCCGCCAGCCTTG
PRIMER_RIGHT_3_SEQUENCE=CTCAGCCTGACGGTCATCAT
PRIMER_INTERNAL_3_SEQUENCE=CCTCTCCCTCAACTCACC
PRIMER_LEFT_3=56,18
PRIMER_RIGHT_3=120,20
PRIMER_INTERNAL_3=83,18
PRIMER_LEFT_3_TM=61.507
PRIMER_RIGHT_3_TM=61.251
PRIMER_INTERNAL_3_TM=55.425
PRIMER_LEFT_3_GC_PERCENT=61.111
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=65
PRIMER_PAIR_3_PRODUCT_TM=76.5
PRIMER_PAIR_4_PENALTY=1.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_INTERNAL_4_PENALTY=1.000000
PRIMER_LEFT_4_SEQUENCE=AAGACTCCGCCAGCCTTG
PRIMER_RIGHT_4_SEQUENCE=CCTCAGCCTGACGGTCATC
PRIMER_INTERNAL_4_SEQUENCE=CTCTCCCTCAACTCACCA
PRIMER_LEFT_4=56,18
PRIMER_RIGHT_4=121,19
PRIMER_INTERNAL_4=84,18
PRIMER_LEFT_4_TM=61.507
PRIMER_RIGHT_4_TM=61.836
PRIMER_INTERNAL_4_TM=54.310
PRIMER_LEFT_4_GC_PERCENT=61.111
PRIMER_RIGHT_4_GC_PERCENT=63.158
PRIMER_INTERNAL_4_GC_PERCENT=55.556
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=66
PRIMER_PAIR_4_PRODUCT_TM=76.9
=
SEQUENCE_ID=diff_tm
PRIMER_PAIR_WT_IO_PENALTY=0
PRIMER_PAIR_WT_PR_PENALTY=1
PRIMER_PAIR_WT_DIFF_TM=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_TASK=pick_pcr_primers
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, high any compl 1, high end compl 72, ok 108
PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, high any compl 1, high end compl 47, ok 76
PRIMER_PAIR_EXPLAIN=considered 10429, unacceptable product size 5893, high end compl 435, ok 4101
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.000039
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=AGCCGAGGATGACTTCACTTTC
PRIMER_RIGHT_0_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
PRIMER_LEFT_0=1,22
PRIMER_RIGHT_0=103,21
PRIMER_LEFT_0_TM=62.010
PRIMER_RIGHT_0_TM=62.010
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=57.143
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=103
PRIMER_PAIR_0_PRODUCT_TM=79.2
PRIMER_PAIR_1_PENALTY=0.005276
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=AAGCCGAGGATGACTTCACTTT
PRIMER_RIGHT_1_SEQUENCE=TCATCATGGTGAGTTGAGGGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=107,21
PRIMER_LEFT_1_TM=61.489
PRIMER_RIGHT_1_TM=61.484
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=108
PRIMER_PAIR_1_PRODUCT_TM=78.6
PRIMER_PAIR_2_PENALTY=0.005368
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=ACGAGTGAAGACTCCGCCA
PRIMER_RIGHT_2_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
PRIMER_LEFT_2=49,19
PRIMER_RIGHT_2=103,21
PRIMER_LEFT_2_TM=62.005
PRIMER_RIGHT_2_TM=62.010
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=55
PRIMER_PAIR_2_PRODUCT_TM=75.1
PRIMER_PAIR_3_PENALTY=0.005368
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=CACGAGTGAAGACTCCGCC
PRIMER_RIGHT_3_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
PRIMER_LEFT_3=48,19
PRIMER_RIGHT_3=103,21
PRIMER_LEFT_3_TM=62.005
PRIMER_RIGHT_3_TM=62.010
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=56
PRIMER_PAIR_3_PRODUCT_TM=75.5
PRIMER_PAIR_4_PENALTY=0.005368
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=CCACGAGTGAAGACTCCGC
PRIMER_RIGHT_4_SEQUENCE=CATGGTGAGTTGAGGGAGAGG
PRIMER_LEFT_4=47,19
PRIMER_RIGHT_4=103,21
PRIMER_LEFT_4_TM=62.005
PRIMER_RIGHT_4_TM=62.010
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=57
PRIMER_PAIR_4_PRODUCT_TM=76.0
=
SEQUENCE_ID=num_NS
PRIMER_PAIR_WT_DIFF_TM=0
PRIMER_WT_NUM_NS=1
PRIMER_MAX_NS_ACCEPTED=15
SEQUENCE_TEMPLATE=AAGCCGNGGATGANTTCACTTTCNGACCNTGCAGAGCCGANTTTCACNCACGAGNGAAGACTNCGCCAGCCTTGCCCCTNGGGCCTCTNCCTCAACTCNCCATGATGNCCGTCAGGNNNNNNNNNCAGGCGNACTGAGGCGTGGTNCGCGGGGCTCAGGNNNNNNNNNCCTGCGCCGG
PRIMER_LEFT_EXPLAIN=considered 1084, GC content failed 4, low tm 61, high tm 810, high end compl 20, long poly-x seq 28, ok 161
PRIMER_RIGHT_EXPLAIN=considered 991, GC content failed 67, low tm 30, high tm 733, high end compl 10, long poly-x seq 45, ok 106
PRIMER_PAIR_EXPLAIN=considered 701, unacceptable product size 590, ok 111
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.000000
PRIMER_LEFT_0_PENALTY=1.000000
PRIMER_RIGHT_0_PENALTY=1.000000
PRIMER_LEFT_0_SEQUENCE=TGCAGAGCCGANTTTCAC
PRIMER_RIGHT_0_SEQUENCE=GAGTTGAGGNAGAGGCCC
PRIMER_LEFT_0=29,18
PRIMER_RIGHT_0=97,18
PRIMER_LEFT_0_TM=58.508
PRIMER_RIGHT_0_TM=57.993
PRIMER_LEFT_0_GC_PERCENT=52.941
PRIMER_RIGHT_0_GC_PERCENT=64.706
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.50
PRIMER_PAIR_0_PRODUCT_SIZE=69
PRIMER_PAIR_0_PRODUCT_TM=74.4
PRIMER_PAIR_1_PENALTY=3.000000
PRIMER_LEFT_1_PENALTY=1.000000
PRIMER_RIGHT_1_PENALTY=2.000000
PRIMER_LEFT_1_SEQUENCE=GGGCCTCTNCCTCAACTC
PRIMER_RIGHT_1_SEQUENCE=GNACCACGCCTCAGTNCG
PRIMER_LEFT_1=80,18
PRIMER_RIGHT_1=146,18
PRIMER_LEFT_1_TM=57.993
PRIMER_RIGHT_1_TM=61.904
PRIMER_LEFT_1_GC_PERCENT=64.706
PRIMER_RIGHT_1_GC_PERCENT=68.750
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.7500
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=67
PRIMER_PAIR_1_PRODUCT_TM=72.4
PRIMER_PAIR_2_PENALTY=3.000000
PRIMER_LEFT_2_PENALTY=1.000000
PRIMER_RIGHT_2_PENALTY=2.000000
PRIMER_LEFT_2_SEQUENCE=GGGCCTCTNCCTCAACTC
PRIMER_RIGHT_2_SEQUENCE=CGNACCACGCCTCAGTNC
PRIMER_LEFT_2=80,18
PRIMER_RIGHT_2=147,18
PRIMER_LEFT_2_TM=57.993
PRIMER_RIGHT_2_TM=61.904
PRIMER_LEFT_2_GC_PERCENT=64.706
PRIMER_RIGHT_2_GC_PERCENT=68.750
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7500
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PAIR_2_PRODUCT_SIZE=68
PRIMER_PAIR_2_PRODUCT_TM=72.8
PRIMER_PAIR_3_PENALTY=3.000000
PRIMER_LEFT_3_PENALTY=1.000000
PRIMER_RIGHT_3_PENALTY=2.000000
PRIMER_LEFT_3_SEQUENCE=AAGACTNCGCCAGCCTTG
PRIMER_RIGHT_3_SEQUENCE=ATCATGGNGAGTTGAGGNAG
PRIMER_LEFT_3=56,18
PRIMER_RIGHT_3=105,20
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=57.730
PRIMER_LEFT_3_GC_PERCENT=58.824
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.50
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5500
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PAIR_3_PRODUCT_SIZE=50
PRIMER_PAIR_3_PRODUCT_TM=70.9
PRIMER_PAIR_4_PENALTY=3.000000
PRIMER_LEFT_4_PENALTY=1.000000
PRIMER_RIGHT_4_PENALTY=2.000000
PRIMER_LEFT_4_SEQUENCE=AAGACTNCGCCAGCCTTG
PRIMER_RIGHT_4_SEQUENCE=ATCATGGNGAGTTGAGGNAGA
PRIMER_LEFT_4=56,18
PRIMER_RIGHT_4=105,21
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=59.709
PRIMER_LEFT_4_GC_PERCENT=58.824
PRIMER_RIGHT_4_GC_PERCENT=47.368
PRIMER_LEFT_4_SELF_ANY=5.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0500
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=50
PRIMER_PAIR_4_PRODUCT_TM=70.9
=
SEQUENCE_ID=product_size
PRIMER_WT_NUM_NS=0
PRIMER_PRODUCT_OPT_SIZE=60
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PAIR_WT_DIFF_TM=0
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
PRIMER_WT_TM_GT=0
PRIMER_WT_TM_LT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_SIZE_GT=0
PRIMER_WT_LIBRARY_MISPRIMING=0
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_MISPRIMING_LIBRARY=
PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182
PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166
PRIMER_PAIR_EXPLAIN=considered 607, unacceptable product size 70, tm diff too large 324, ok 213
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=ACTTTCCGACCCTGCAGAGCC
PRIMER_RIGHT_0_SEQUENCE=GGGCAAGGCTGGCGGAGT
PRIMER_LEFT_0=17,21
PRIMER_RIGHT_0=76,18
PRIMER_LEFT_0_TM=67.188
PRIMER_RIGHT_0_TM=68.249
PRIMER_LEFT_0_GC_PERCENT=61.905
PRIMER_RIGHT_0_GC_PERCENT=72.222
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=60
PRIMER_PAIR_0_PRODUCT_TM=75.2
PRIMER_PAIR_1_PENALTY=0.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=ACTTTCCGACCCTGCAGAGCC
PRIMER_RIGHT_1_SEQUENCE=GGGCAAGGCTGGCGGAGTC
PRIMER_LEFT_1=17,21
PRIMER_RIGHT_1=76,19
PRIMER_LEFT_1_TM=67.188
PRIMER_RIGHT_1_TM=69.823
PRIMER_LEFT_1_GC_PERCENT=61.905
PRIMER_RIGHT_1_GC_PERCENT=73.684
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=60
PRIMER_PAIR_1_PRODUCT_TM=75.2
PRIMER_PAIR_2_PENALTY=0.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=CACTTTCCGACCCTGCAGAGC
PRIMER_RIGHT_2_SEQUENCE=GGCAAGGCTGGCGGAGTC
PRIMER_LEFT_2=16,21
PRIMER_RIGHT_2=75,18
PRIMER_LEFT_2_TM=66.655
PRIMER_RIGHT_2_TM=66.706
PRIMER_LEFT_2_GC_PERCENT=61.905
PRIMER_RIGHT_2_GC_PERCENT=72.222
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=60
PRIMER_PAIR_2_PRODUCT_TM=75.2
PRIMER_PAIR_3_PENALTY=0.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=CACTTTCCGACCCTGCAGAGCC
PRIMER_RIGHT_3_SEQUENCE=GGCAAGGCTGGCGGAGTC
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=75,18
PRIMER_LEFT_3_TM=69.546
PRIMER_RIGHT_3_TM=66.706
PRIMER_LEFT_3_GC_PERCENT=63.636
PRIMER_RIGHT_3_GC_PERCENT=72.222
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=60
PRIMER_PAIR_3_PRODUCT_TM=75.2
PRIMER_PAIR_4_PENALTY=0.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=CACTTTCCGACCCTGCAGAGC
PRIMER_RIGHT_4_SEQUENCE=GGCAAGGCTGGCGGAGTCT
PRIMER_LEFT_4=16,21
PRIMER_RIGHT_4=75,19
PRIMER_LEFT_4_TM=66.655
PRIMER_RIGHT_4_TM=67.348
PRIMER_LEFT_4_GC_PERCENT=61.905
PRIMER_RIGHT_4_GC_PERCENT=68.421
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=60
PRIMER_PAIR_4_PRODUCT_TM=75.2
=
SEQUENCE_ID=rep_sim
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
PRIMER_MAX_LIBRARY_MISPRIMING=20
PRIMER_WT_LIBRARY_MISPRIMING=1
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182
PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166
PRIMER_PAIR_EXPLAIN=considered 624, unacceptable product size 163, tm diff too large 320, ok 141
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=14.000000
PRIMER_LEFT_0_PENALTY=7.000000
PRIMER_RIGHT_0_PENALTY=7.000000
PRIMER_LEFT_0_SEQUENCE=GGATGACTTCACTTTCCGACC
PRIMER_RIGHT_0_SEQUENCE=GGCTGGCGGAGTCTTCAC
PRIMER_LEFT_0=7,21
PRIMER_RIGHT_0=70,18
PRIMER_LEFT_0_TM=60.878
PRIMER_RIGHT_0_TM=61.981
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=64
PRIMER_PAIR_0_PRODUCT_TM=74.9
PRIMER_PAIR_1_PENALTY=14.000000
PRIMER_LEFT_1_PENALTY=7.000000
PRIMER_RIGHT_1_PENALTY=7.000000
PRIMER_LEFT_1_SEQUENCE=GGATGACTTCACTTTCCGACC
PRIMER_RIGHT_1_SEQUENCE=AGGCTGGCGGAGTCTTCA
PRIMER_LEFT_1=7,21
PRIMER_RIGHT_1=71,18
PRIMER_LEFT_1_TM=60.878
PRIMER_RIGHT_1_TM=62.110
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=65
PRIMER_PAIR_1_PRODUCT_TM=74.6
PRIMER_PAIR_2_PENALTY=14.000000
PRIMER_LEFT_2_PENALTY=7.000000
PRIMER_RIGHT_2_PENALTY=7.000000
PRIMER_LEFT_2_SEQUENCE=GGATGACTTCACTTTCCGACC
PRIMER_RIGHT_2_SEQUENCE=AGGCTGGCGGAGTCTTCAC
PRIMER_LEFT_2=7,21
PRIMER_RIGHT_2=71,19
PRIMER_LEFT_2_TM=60.878
PRIMER_RIGHT_2_TM=62.895
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=63.158
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_2_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=65
PRIMER_PAIR_2_PRODUCT_TM=74.6
PRIMER_PAIR_3_PENALTY=14.000000
PRIMER_LEFT_3_PENALTY=7.000000
PRIMER_RIGHT_3_PENALTY=7.000000
PRIMER_LEFT_3_SEQUENCE=GGATGACTTCACTTTCCGACC
PRIMER_RIGHT_3_SEQUENCE=AAGGCTGGCGGAGTCTTCA
PRIMER_LEFT_3=7,21
PRIMER_RIGHT_3=72,19
PRIMER_LEFT_3_TM=60.878
PRIMER_RIGHT_3_TM=63.377
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=66
PRIMER_PAIR_3_PRODUCT_TM=74.4
PRIMER_PAIR_4_PENALTY=15.000000
PRIMER_LEFT_4_PENALTY=8.000000
PRIMER_RIGHT_4_PENALTY=7.000000
PRIMER_LEFT_4_SEQUENCE=TTTCCGACCCTGCAGAGC
PRIMER_RIGHT_4_SEQUENCE=GGCTGGCGGAGTCTTCAC
PRIMER_LEFT_4=19,18
PRIMER_RIGHT_4=70,18
PRIMER_LEFT_4_TM=62.504
PRIMER_RIGHT_4_TM=61.981
PRIMER_LEFT_4_GC_PERCENT=61.111
PRIMER_RIGHT_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_4_LIBRARY_MISPRIMING=15.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=52
PRIMER_PAIR_4_PRODUCT_TM=73.6
=
SEQUENCE_ID=pair_rep_sim
PRIMER_WT_LIBRARY_MISPRIMING=0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC
PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182
PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166
PRIMER_PAIR_EXPLAIN=considered 30212, unacceptable product size 14224, tm diff too large 10477, ok 5511
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=14.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=TTTCCGACCCTGCAGAGC
PRIMER_RIGHT_0_SEQUENCE=CTGGCGGAGTCTTCACTCG
PRIMER_LEFT_0=19,18
PRIMER_RIGHT_0=68,19
PRIMER_LEFT_0_TM=62.504
PRIMER_RIGHT_0_TM=62.132
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=50
PRIMER_PAIR_0_PRODUCT_TM=72.5
PRIMER_PAIR_1_PENALTY=14.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=TTTCCGACCCTGCAGAGC
PRIMER_RIGHT_1_SEQUENCE=AGGCTGGCGGAGTCTTCA
PRIMER_LEFT_1=19,18
PRIMER_RIGHT_1=71,18
PRIMER_LEFT_1_TM=62.504
PRIMER_RIGHT_1_TM=62.110
PRIMER_LEFT_1_GC_PERCENT=61.111
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=53
PRIMER_PAIR_1_PRODUCT_TM=73.3
PRIMER_PAIR_2_PENALTY=14.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=CTTTCCGACCCTGCAGAGC
PRIMER_RIGHT_2_SEQUENCE=TGGCGGAGTCTTCACTCG
PRIMER_LEFT_2=18,19
PRIMER_RIGHT_2=67,18
PRIMER_LEFT_2_TM=63.377
PRIMER_RIGHT_2_TM=61.148
PRIMER_LEFT_2_GC_PERCENT=63.158
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_2_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=50
PRIMER_PAIR_2_PRODUCT_TM=72.5
PRIMER_PAIR_3_PENALTY=14.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=CTTTCCGACCCTGCAGAGC
PRIMER_RIGHT_3_SEQUENCE=CTGGCGGAGTCTTCACTCG
PRIMER_LEFT_3=18,19
PRIMER_RIGHT_3=68,19
PRIMER_LEFT_3_TM=63.377
PRIMER_RIGHT_3_TM=62.132
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_3_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=51
PRIMER_PAIR_3_PRODUCT_TM=73.1
PRIMER_PAIR_4_PENALTY=14.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=CTTTCCGACCCTGCAGAGC
PRIMER_RIGHT_4_SEQUENCE=GCTGGCGGAGTCTTCACTCG
PRIMER_LEFT_4=18,19
PRIMER_RIGHT_4=69,20
PRIMER_LEFT_4_TM=63.377
PRIMER_RIGHT_4_TM=65.780
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=65.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_4_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=52
PRIMER_PAIR_4_PRODUCT_TM=73.6
=
SEQUENCE_ID=product_tm
PRIMER_PAIR_WT_PRODUCT_TM_LT=1
PRIMER_PAIR_WT_PRODUCT_TM_GT=1
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0
PRIMER_PRODUCT_OPT_TM=80
PRIMER_MISPRIMING_LIBRARY=
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCT
PRIMER_LEFT_EXPLAIN=considered 370, low tm 2, high tm 145, ok 223
PRIMER_RIGHT_EXPLAIN=considered 370, GC content failed 6, high tm 198, ok 166
PRIMER_PAIR_EXPLAIN=considered 37018, unacceptable product size 21030, tm diff too large 10477, ok 5511
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.950431
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=GCCGAGGATGACTTCACTTTCCG
PRIMER_RIGHT_0_SEQUENCE=GGGCAAGGCTGGCGGAGT
PRIMER_LEFT_0=2,23
PRIMER_RIGHT_0=76,18
PRIMER_LEFT_0_TM=68.296
PRIMER_RIGHT_0_TM=68.249
PRIMER_LEFT_0_GC_PERCENT=56.522
PRIMER_RIGHT_0_GC_PERCENT=72.222
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=10.2000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_0_PRODUCT_TM=77.0
PRIMER_PAIR_1_PENALTY=2.950431
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=GCCGAGGATGACTTCACTTTCCG
PRIMER_RIGHT_1_SEQUENCE=GGGCAAGGCTGGCGGAGTC
PRIMER_LEFT_1=2,23
PRIMER_RIGHT_1=76,19
PRIMER_LEFT_1_TM=68.296
PRIMER_RIGHT_1_TM=69.823
PRIMER_LEFT_1_GC_PERCENT=56.522
PRIMER_RIGHT_1_GC_PERCENT=73.684
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_1_PRODUCT_TM=77.0
PRIMER_PAIR_2_PENALTY=2.950431
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=GCCGAGGATGACTTCACTTTCCGA
PRIMER_RIGHT_2_SEQUENCE=GGGCAAGGCTGGCGGAGT
PRIMER_LEFT_2=2,24
PRIMER_RIGHT_2=76,18
PRIMER_LEFT_2_TM=69.669
PRIMER_RIGHT_2_TM=68.249
PRIMER_LEFT_2_GC_PERCENT=54.167
PRIMER_RIGHT_2_GC_PERCENT=72.222
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_2_PRODUCT_TM=77.0
PRIMER_PAIR_3_PENALTY=2.950431
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=GCCGAGGATGACTTCACTTTCCGA
PRIMER_RIGHT_3_SEQUENCE=GGGCAAGGCTGGCGGAGTC
PRIMER_LEFT_3=2,24
PRIMER_RIGHT_3=76,19
PRIMER_LEFT_3_TM=69.669
PRIMER_RIGHT_3_TM=69.823
PRIMER_LEFT_3_GC_PERCENT=54.167
PRIMER_RIGHT_3_GC_PERCENT=73.684
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_3_PRODUCT_TM=77.0
PRIMER_PAIR_4_PENALTY=3.176045
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=AGCCGAGGATGACTTCACTTTCCG
PRIMER_RIGHT_4_SEQUENCE=GGGCAAGGCTGGCGGAGT
PRIMER_LEFT_4=1,24
PRIMER_RIGHT_4=76,18
PRIMER_LEFT_4_TM=68.778
PRIMER_RIGHT_4_TM=68.249
PRIMER_LEFT_4_GC_PERCENT=54.167
PRIMER_RIGHT_4_GC_PERCENT=72.222
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
PRIMER_PAIR_4_PRODUCT_TM=76.8
=
SEQUENCE_ID=primer_compl
PRIMER_MIN_GC=1.0
PRIMER_MAX_GC=99.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0
PRIMER_PRODUCT_OPT_SIZE=200
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
PRIMER_PRODUCT_OPT_TM=0
PRIMER_WT_LIBRARY_MISPRIMING=0
PRIMER_WT_SELF_ANY=1
PRIMER_WT_SELF_END=2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAATAGACATCATAATTAGATACAC
PRIMER_LEFT_EXPLAIN=considered 814, low tm 177, long poly-x seq 23, ok 614
PRIMER_RIGHT_EXPLAIN=considered 940, low tm 244, ok 696
PRIMER_PAIR_EXPLAIN=considered 60, unacceptable product size 33, tm diff too large 10, ok 17
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.000000
PRIMER_LEFT_0_PENALTY=2.000000
PRIMER_RIGHT_0_PENALTY=2.000000
PRIMER_LEFT_0_SEQUENCE=AAGGTAAAAATGAGGTATGAA
PRIMER_RIGHT_0_SEQUENCE=TGTCTATTTGTTGGAGGAA
PRIMER_LEFT_0=68,21
PRIMER_RIGHT_0=126,19
PRIMER_LEFT_0_TM=50.931
PRIMER_RIGHT_0_TM=51.365
PRIMER_LEFT_0_GC_PERCENT=28.571
PRIMER_RIGHT_0_GC_PERCENT=36.842
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=59
PRIMER_PAIR_0_PRODUCT_TM=62.9
PRIMER_PAIR_1_PENALTY=4.000000
PRIMER_LEFT_1_PENALTY=2.000000
PRIMER_RIGHT_1_PENALTY=2.000000
PRIMER_LEFT_1_SEQUENCE=AAGGTAAAAATGAGGTATGAA
PRIMER_RIGHT_1_SEQUENCE=ATGTCTATTTGTTGGAGGA
PRIMER_LEFT_1=68,21
PRIMER_RIGHT_1=127,19
PRIMER_LEFT_1_TM=50.931
PRIMER_RIGHT_1_TM=50.179
PRIMER_LEFT_1_GC_PERCENT=28.571
PRIMER_RIGHT_1_GC_PERCENT=36.842
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=60
PRIMER_PAIR_1_PRODUCT_TM=62.9
PRIMER_PAIR_2_PENALTY=4.000000
PRIMER_LEFT_2_PENALTY=2.000000
PRIMER_RIGHT_2_PENALTY=2.000000
PRIMER_LEFT_2_SEQUENCE=AAGGTAAAAATGAGGTATGAA
PRIMER_RIGHT_2_SEQUENCE=ATGTCTATTTGTTGGAGGAA
PRIMER_LEFT_2=68,21
PRIMER_RIGHT_2=127,20
PRIMER_LEFT_2_TM=50.931
PRIMER_RIGHT_2_TM=52.153
PRIMER_LEFT_2_GC_PERCENT=28.571
PRIMER_RIGHT_2_GC_PERCENT=35.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=60
PRIMER_PAIR_2_PRODUCT_TM=62.9
PRIMER_PAIR_3_PENALTY=4.000000
PRIMER_LEFT_3_PENALTY=2.000000
PRIMER_RIGHT_3_PENALTY=2.000000
PRIMER_LEFT_3_SEQUENCE=AAGGTAAAAATGAGGTATGAA
PRIMER_RIGHT_3_SEQUENCE=GATGTCTATTTGTTGGAGG
PRIMER_LEFT_3=68,21
PRIMER_RIGHT_3=128,19
PRIMER_LEFT_3_TM=50.931
PRIMER_RIGHT_3_TM=50.179
PRIMER_LEFT_3_GC_PERCENT=28.571
PRIMER_RIGHT_3_GC_PERCENT=42.105
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=61
PRIMER_PAIR_3_PRODUCT_TM=63.5
PRIMER_PAIR_4_PENALTY=4.000000
PRIMER_LEFT_4_PENALTY=2.000000
PRIMER_RIGHT_4_PENALTY=2.000000
PRIMER_LEFT_4_SEQUENCE=AAGGTAAAAATGAGGTATGAA
PRIMER_RIGHT_4_SEQUENCE=GATGTCTATTTGTTGGAGGA
PRIMER_LEFT_4=68,21
PRIMER_RIGHT_4=128,20
PRIMER_LEFT_4_TM=50.931
PRIMER_RIGHT_4_TM=52.504
PRIMER_LEFT_4_GC_PERCENT=28.571
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=61
PRIMER_PAIR_4_PRODUCT_TM=63.5
=
PRIMER_MISPRIMING_LIBRARY=
PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_WT_SELF_ANY=0
PRIMER_WT_SELF_END=0
PRIMER_INTERNAL_WT_TM_GT=0
PRIMER_INTERNAL_WT_TM_LT=0
PRIMER_INTERNAL_WT_SIZE_LT=0
PRIMER_INTERNAL_WT_SIZE_GT=0
PRIMER_INTERNAL_WT_SELF_ANY=0
PRIMER_INTERNAL_WT_SELF_END=0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=1
PRIMER_PAIR_WT_COMPL_ANY=0
PRIMER_PAIR_WT_COMPL_END=0
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=20
PRIMER_PAIR_WT_PR_PENALTY=0
PRIMER_PAIR_WT_IO_PENALTY=1
SEQUENCE_ID=io_rep_sim
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATAACCTATACATAATGTGAAATATAATTTTT
PRIMER_LEFT_EXPLAIN=considered 584, low tm 82, long poly-x seq 23, ok 479
PRIMER_RIGHT_EXPLAIN=considered 710, low tm 257, ok 453
PRIMER_INTERNAL_EXPLAIN=considered 859, GC content failed 67, low tm 223, long poly-x seq 23, ok 546
PRIMER_PAIR_EXPLAIN=considered 216987, unacceptable product size 157643, tm diff too large 48119, no internal oligo 8093, ok 3132
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_INTERNAL_0_PENALTY=8.000000
PRIMER_LEFT_0_SEQUENCE=GCCCAAATCCCATTATTC
PRIMER_RIGHT_0_SEQUENCE=ATGTATAGGTTATTCATACCTCATT
PRIMER_INTERNAL_0_SEQUENCE=ATAATGCATTTCAAGGTAAA
PRIMER_LEFT_0=38,18
PRIMER_RIGHT_0=100,25
PRIMER_INTERNAL_0=56,20
PRIMER_LEFT_0_TM=55.228
PRIMER_RIGHT_0_TM=52.436
PRIMER_INTERNAL_0_TM=50.207
PRIMER_LEFT_0_GC_PERCENT=44.444
PRIMER_RIGHT_0_GC_PERCENT=28.000
PRIMER_INTERNAL_0_GC_PERCENT=25.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_INTERNAL_0_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=63
PRIMER_PAIR_0_PRODUCT_TM=63.4
PRIMER_PAIR_1_PENALTY=8.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_INTERNAL_1_PENALTY=8.000000
PRIMER_LEFT_1_SEQUENCE=GCCCAAATCCCATTATTC
PRIMER_RIGHT_1_SEQUENCE=TATGTATAGGTTATTCATACCTCATT
PRIMER_INTERNAL_1_SEQUENCE=ATAATGCATTTCAAGGTAAA
PRIMER_LEFT_1=38,18
PRIMER_RIGHT_1=101,26
PRIMER_INTERNAL_1=56,20
PRIMER_LEFT_1_TM=55.228
PRIMER_RIGHT_1_TM=52.684
PRIMER_INTERNAL_1_TM=50.207
PRIMER_LEFT_1_GC_PERCENT=44.444
PRIMER_RIGHT_1_GC_PERCENT=26.923
PRIMER_INTERNAL_1_GC_PERCENT=25.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=9.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_INTERNAL_1_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=64
PRIMER_PAIR_1_PRODUCT_TM=63.3
PRIMER_PAIR_2_PENALTY=8.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_INTERNAL_2_PENALTY=8.000000
PRIMER_LEFT_2_SEQUENCE=GCCCAAATCCCATTATTC
PRIMER_RIGHT_2_SEQUENCE=TTATGTATAGGTTATTCATACCTCAT
PRIMER_INTERNAL_2_SEQUENCE=ATAATGCATTTCAAGGTAAA
PRIMER_LEFT_2=38,18
PRIMER_RIGHT_2=102,26
PRIMER_INTERNAL_2=56,20
PRIMER_LEFT_2_TM=55.228
PRIMER_RIGHT_2_TM=52.684
PRIMER_INTERNAL_2_TM=50.207
PRIMER_LEFT_2_GC_PERCENT=44.444
PRIMER_RIGHT_2_GC_PERCENT=26.923
PRIMER_INTERNAL_2_GC_PERCENT=25.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=9.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_INTERNAL_2_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=65
PRIMER_PAIR_2_PRODUCT_TM=63.3
PRIMER_PAIR_3_PENALTY=8.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_INTERNAL_3_PENALTY=8.000000
PRIMER_LEFT_3_SEQUENCE=GCCCAAATCCCATTATTC
PRIMER_RIGHT_3_SEQUENCE=TTATGTATAGGTTATTCATACCTCATT
PRIMER_INTERNAL_3_SEQUENCE=ATAATGCATTTCAAGGTAAA
PRIMER_LEFT_3=38,18
PRIMER_RIGHT_3=102,27
PRIMER_INTERNAL_3=56,20
PRIMER_LEFT_3_TM=55.228
PRIMER_RIGHT_3_TM=54.045
PRIMER_INTERNAL_3_TM=50.207
PRIMER_LEFT_3_GC_PERCENT=44.444
PRIMER_RIGHT_3_GC_PERCENT=25.926
PRIMER_INTERNAL_3_GC_PERCENT=25.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=9.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_INTERNAL_3_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=65
PRIMER_PAIR_3_PRODUCT_TM=63.3
PRIMER_PAIR_4_PENALTY=8.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_INTERNAL_4_PENALTY=8.000000
PRIMER_LEFT_4_SEQUENCE=GCCCAAATCCCATTATTC
PRIMER_RIGHT_4_SEQUENCE=ATTATGTATAGGTTATTCATACCTCA
PRIMER_INTERNAL_4_SEQUENCE=ATAATGCATTTCAAGGTAAA
PRIMER_LEFT_4=38,18
PRIMER_RIGHT_4=103,26
PRIMER_INTERNAL_4=56,20
PRIMER_LEFT_4_TM=55.228
PRIMER_RIGHT_4_TM=52.684
PRIMER_INTERNAL_4_TM=50.207
PRIMER_LEFT_4_GC_PERCENT=44.444
PRIMER_RIGHT_4_GC_PERCENT=26.923
PRIMER_INTERNAL_4_GC_PERCENT=25.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=9.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_INTERNAL_4_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=66
PRIMER_PAIR_4_PRODUCT_TM=63.2
=
SEQUENCE_ID=end_stability
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_INTERNAL_MISHYB_LIBRARY=
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PAIR_WT_PR_PENALTY=1
PRIMER_PAIR_WT_IO_PENALTY=0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0
PRIMER_WT_END_STABILITY=1
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCG
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_LEFT_EXPLAIN=considered 1130, low tm 2, high tm 643, ok 485
PRIMER_RIGHT_EXPLAIN=considered 1130, high tm 722, ok 408
PRIMER_PAIR_EXPLAIN=considered 1048, unacceptable product size 646, tm diff too large 287, ok 115
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=12.500000
PRIMER_LEFT_0_PENALTY=6.100000
PRIMER_RIGHT_0_PENALTY=6.400000
PRIMER_LEFT_0_SEQUENCE=ACCCACGAGTGAAGACTC
PRIMER_RIGHT_0_SEQUENCE=TGGTGAGTTGAGGGAGAG
PRIMER_LEFT_0=45,18
PRIMER_RIGHT_0=101,18
PRIMER_LEFT_0_TM=53.119
PRIMER_RIGHT_0_TM=54.310
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=55.556
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=57
PRIMER_PAIR_0_PRODUCT_TM=76.0
PRIMER_PAIR_1_PENALTY=12.500000
PRIMER_LEFT_1_PENALTY=6.100000
PRIMER_RIGHT_1_PENALTY=6.400000
PRIMER_LEFT_1_SEQUENCE=ACCCACGAGTGAAGACTC
PRIMER_RIGHT_1_SEQUENCE=ATGGTGAGTTGAGGGAGAG
PRIMER_LEFT_1=45,18
PRIMER_RIGHT_1=102,19
PRIMER_LEFT_1_TM=53.119
PRIMER_RIGHT_1_TM=54.979
PRIMER_LEFT_1_GC_PERCENT=55.556
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=58
PRIMER_PAIR_1_PRODUCT_TM=75.7
PRIMER_PAIR_2_PENALTY=12.500000
PRIMER_LEFT_2_PENALTY=6.100000
PRIMER_RIGHT_2_PENALTY=6.400000
PRIMER_LEFT_2_SEQUENCE=CACCCACGAGTGAAGACT
PRIMER_RIGHT_2_SEQUENCE=TGGTGAGTTGAGGGAGAG
PRIMER_LEFT_2=44,18
PRIMER_RIGHT_2=101,18
PRIMER_LEFT_2_TM=54.245
PRIMER_RIGHT_2_TM=54.310
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=55.556
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=58
PRIMER_PAIR_2_PRODUCT_TM=76.4
PRIMER_PAIR_3_PENALTY=12.500000
PRIMER_LEFT_3_PENALTY=6.100000
PRIMER_RIGHT_3_PENALTY=6.400000
PRIMER_LEFT_3_SEQUENCE=CACCCACGAGTGAAGACTC
PRIMER_RIGHT_3_SEQUENCE=TGGTGAGTTGAGGGAGAG
PRIMER_LEFT_3=44,19
PRIMER_RIGHT_3=101,18
PRIMER_LEFT_3_TM=56.607
PRIMER_RIGHT_3_TM=54.310
PRIMER_LEFT_3_GC_PERCENT=57.895
PRIMER_RIGHT_3_GC_PERCENT=55.556
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=58
PRIMER_PAIR_3_PRODUCT_TM=76.4
PRIMER_PAIR_4_PENALTY=12.500000
PRIMER_LEFT_4_PENALTY=6.100000
PRIMER_RIGHT_4_PENALTY=6.400000
PRIMER_LEFT_4_SEQUENCE=CACCCACGAGTGAAGACT
PRIMER_RIGHT_4_SEQUENCE=ATGGTGAGTTGAGGGAGAG
PRIMER_LEFT_4=44,18
PRIMER_RIGHT_4=102,19
PRIMER_LEFT_4_TM=54.245
PRIMER_RIGHT_4_TM=54.979
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=52.632
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=59
PRIMER_PAIR_4_PRODUCT_TM=76.1
=
SEQUENCE_ID=position_penalty
SEQUENCE_TARGET=298,45
PRIMER_OUTSIDE_PENALTY=20.0
PRIMER_INSIDE_PENALTY=1000.0
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=36-2000
PRIMER_WT_POS_PENALTY=1
PRIMER_WT_END_STABILITY=0
SEQUENCE_TEMPLATE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAA
PRIMER_LEFT_EXPLAIN=considered 3260, overlap target 45, low tm 300, high tm 587, ok 2328
PRIMER_RIGHT_EXPLAIN=considered 1064, overlap target 289, low tm 64, high tm 149, ok 562
PRIMER_PAIR_EXPLAIN=considered 16, tm diff too large 9, ok 7
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=AGCCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_0_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT
PRIMER_LEFT_0=277,21
PRIMER_RIGHT_0=365,23
PRIMER_LEFT_0_TM=64.839
PRIMER_RIGHT_0_TM=67.828
PRIMER_LEFT_0_GC_PERCENT=57.143
PRIMER_RIGHT_0_GC_PERCENT=56.522
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=11.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_0_PRODUCT_TM=73.0
PRIMER_PAIR_1_PENALTY=0.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=CAGCCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_1_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT
PRIMER_LEFT_1=276,22
PRIMER_RIGHT_1=365,23
PRIMER_LEFT_1_TM=67.212
PRIMER_RIGHT_1_TM=67.828
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=56.522
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_1_PRODUCT_TM=73.3
PRIMER_PAIR_2_PENALTY=0.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=CCAGCCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_2_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT
PRIMER_LEFT_2=275,23
PRIMER_RIGHT_2=365,23
PRIMER_LEFT_2_TM=69.870
PRIMER_RIGHT_2_TM=67.828
PRIMER_LEFT_2_GC_PERCENT=60.870
PRIMER_RIGHT_2_GC_PERCENT=56.522
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_2_PRODUCT_TM=73.6
PRIMER_PAIR_3_PENALTY=0.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=GCCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_3_SEQUENCE=AAGGGGAAAGCTCACCCCTCTT
PRIMER_LEFT_3=278,20
PRIMER_RIGHT_3=364,22
PRIMER_LEFT_3_TM=64.136
PRIMER_RIGHT_3_TM=65.600
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=87
PRIMER_PAIR_3_PRODUCT_TM=72.8
PRIMER_PAIR_4_PENALTY=0.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=AGCCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_4_SEQUENCE=AAGGGGAAAGCTCACCCCTCTT
PRIMER_LEFT_4=277,21
PRIMER_RIGHT_4=364,22
PRIMER_LEFT_4_TM=64.839
PRIMER_RIGHT_4_TM=65.600
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
PRIMER_PAIR_4_PRODUCT_TM=72.7
=
SEQUENCE_ID=sequence_quality
PRIMER_WT_POS_PENALTY=0
PRIMER_WT_SEQ_QUAL=1
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_QUALITY_RANGE_MAX=70
SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 20 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
PRIMER_LEFT_EXPLAIN=considered 720, low tm 219, ok 501
PRIMER_RIGHT_EXPLAIN=considered 720, low tm 282, ok 438
PRIMER_PAIR_EXPLAIN=considered 54742, unacceptable product size 32903, tm diff too large 13218, high any compl 329, ok 8292
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=40.000000
PRIMER_LEFT_0_PENALTY=20.000000
PRIMER_RIGHT_0_PENALTY=20.000000
PRIMER_LEFT_0_SEQUENCE=TGATCATCGTAGCTAGCTAG
PRIMER_RIGHT_0_SEQUENCE=ATGACTAGTAGCTACGTAGTACG
PRIMER_LEFT_0=52,20
PRIMER_RIGHT_0=101,23
PRIMER_LEFT_0_TM=50.931
PRIMER_RIGHT_0_TM=50.484
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=10.00
PRIMER_RIGHT_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=8.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=50
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=50
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=10.00
PRIMER_PAIR_0_COMPL_END=7.00
PRIMER_PAIR_0_PRODUCT_SIZE=50
PRIMER_PAIR_0_PRODUCT_TM=65.1
PRIMER_PAIR_1_PENALTY=40.000000
PRIMER_LEFT_1_PENALTY=20.000000
PRIMER_RIGHT_1_PENALTY=20.000000
PRIMER_LEFT_1_SEQUENCE=TGATCATCGTAGCTAGCTAG
PRIMER_RIGHT_1_SEQUENCE=ATGACTAGTAGCTACGTAGTACGA
PRIMER_LEFT_1=52,20
PRIMER_RIGHT_1=101,24
PRIMER_LEFT_1_TM=50.931
PRIMER_RIGHT_1_TM=52.419
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=8.00
PRIMER_RIGHT_1_SELF_END=10.00
PRIMER_LEFT_1_MIN_SEQ_QUALITY=50
PRIMER_RIGHT_1_MIN_SEQ_QUALITY=50
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=10.00
PRIMER_PAIR_1_COMPL_END=7.00
PRIMER_PAIR_1_PRODUCT_SIZE=50
PRIMER_PAIR_1_PRODUCT_TM=65.1
PRIMER_PAIR_2_PENALTY=40.000000
PRIMER_LEFT_2_PENALTY=20.000000
PRIMER_RIGHT_2_PENALTY=20.000000
PRIMER_LEFT_2_SEQUENCE=TGATCATCGTAGCTAGCTAG
PRIMER_RIGHT_2_SEQUENCE=ATGACTAGTAGCTACGTAGTACGAT
PRIMER_LEFT_2=52,20
PRIMER_RIGHT_2=101,25
PRIMER_LEFT_2_TM=50.931
PRIMER_RIGHT_2_TM=53.013
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=12.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=10.00
PRIMER_LEFT_2_MIN_SEQ_QUALITY=50
PRIMER_RIGHT_2_MIN_SEQ_QUALITY=50
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=10.00
PRIMER_PAIR_2_COMPL_END=8.00
PRIMER_PAIR_2_PRODUCT_SIZE=50
PRIMER_PAIR_2_PRODUCT_TM=65.1
PRIMER_PAIR_3_PENALTY=40.000000
PRIMER_LEFT_3_PENALTY=20.000000
PRIMER_RIGHT_3_PENALTY=20.000000
PRIMER_LEFT_3_SEQUENCE=TGATCATCGTAGCTAGCTAGT
PRIMER_RIGHT_3_SEQUENCE=ATGACTAGTAGCTACGTAGTACG
PRIMER_LEFT_3=52,21
PRIMER_RIGHT_3=101,23
PRIMER_LEFT_3_TM=52.135
PRIMER_RIGHT_3_TM=50.484
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=12.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=8.00
PRIMER_LEFT_3_MIN_SEQ_QUALITY=50
PRIMER_RIGHT_3_MIN_SEQ_QUALITY=50
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=11.00
PRIMER_PAIR_3_COMPL_END=8.00
PRIMER_PAIR_3_PRODUCT_SIZE=50
PRIMER_PAIR_3_PRODUCT_TM=65.1
PRIMER_PAIR_4_PENALTY=40.000000
PRIMER_LEFT_4_PENALTY=20.000000
PRIMER_RIGHT_4_PENALTY=20.000000
PRIMER_LEFT_4_SEQUENCE=TGATCATCGTAGCTAGCTAGT
PRIMER_RIGHT_4_SEQUENCE=ATGACTAGTAGCTACGTAGTACGA
PRIMER_LEFT_4=52,21
PRIMER_RIGHT_4=101,24
PRIMER_LEFT_4_TM=52.135
PRIMER_RIGHT_4_TM=52.419
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=10.00
PRIMER_RIGHT_4_SELF_ANY=12.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=10.00
PRIMER_LEFT_4_MIN_SEQ_QUALITY=50
PRIMER_RIGHT_4_MIN_SEQ_QUALITY=50
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=11.00
PRIMER_PAIR_4_COMPL_END=8.00
PRIMER_PAIR_4_PRODUCT_SIZE=50
PRIMER_PAIR_4_PRODUCT_TM=65.1
=
SEQUENCE_ID=sequence_quality_io
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_WT_SEQ_QUAL=1
PRIMER_PAIR_MAX_DIFF_TM=20
PRIMER_MIN_TM=20
PRIMER_MAX_TM=100
PRIMER_INTERNAL_WT_SELF_ANY=0
PRIMER_INTERNAL_WT_SELF_END=0
PRIMER_INTERNAL_MAX_SELF_ANY=20
PRIMER_INTERNAL_MAX_SELF_END=20
PRIMER_INTERNAL_MIN_TM=20
PRIMER_INTERNAL_MAX_TM=100
PRIMER_MAX_SELF_ANY=20
PRIMER_MAX_SELF_END=20
PRIMER_MAX_SELF_ANY=20
PRIMER_MAX_SELF_END=20
PRIMER_PAIR_MAX_COMPL_ANY=20
PRIMER_PAIR_MAX_COMPL_END=20
PRIMER_INTERNAL_WT_END_QUAL=2
PRIMER_PAIR_WT_PR_PENALTY=0
PRIMER_PAIR_WT_IO_PENALTY=1
PRIMER_WT_SEQ_QUAL=0
PRIMER_WT_END_QUAL=0
SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATC
SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
PRIMER_LEFT_EXPLAIN=considered 310, ok 310
PRIMER_RIGHT_EXPLAIN=considered 310, ok 310
PRIMER_INTERNAL_EXPLAIN=considered 585, ok 585
PRIMER_PAIR_EXPLAIN=considered 96100, unacceptable product size 46500, tm diff too large 938, no internal oligo 30861, ok 17801
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=20.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_INTERNAL_0_PENALTY=20.000000
PRIMER_LEFT_0_SEQUENCE=CGTAGCTGATCGTAGCTG
PRIMER_RIGHT_0_SEQUENCE=ATCAGCTAGATCAGTAGT
PRIMER_INTERNAL_0_SEQUENCE=ATCGTAGCTAGTCAGTCT
PRIMER_LEFT_0=2,18
PRIMER_RIGHT_0=55,18
PRIMER_INTERNAL_0=20,18
PRIMER_LEFT_0_TM=52.936
PRIMER_RIGHT_0_TM=40.383
PRIMER_INTERNAL_0_TM=43.690
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=38.889
PRIMER_INTERNAL_0_GC_PERCENT=44.444
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_MIN_SEQ_QUALITY=30
PRIMER_RIGHT_0_MIN_SEQ_QUALITY=20
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=5.1000
PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=50
PRIMER_PAIR_0_COMPL_ANY=10.00
PRIMER_PAIR_0_COMPL_END=9.00
PRIMER_PAIR_0_PRODUCT_SIZE=54
PRIMER_PAIR_0_PRODUCT_TM=67.8
PRIMER_PAIR_1_PENALTY=20.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_INTERNAL_1_PENALTY=20.000000
PRIMER_LEFT_1_SEQUENCE=CGTAGCTGATCGTAGCTG
PRIMER_RIGHT_1_SEQUENCE=GATCAGCTAGATCAGTAG
PRIMER_INTERNAL_1_SEQUENCE=ATCGTAGCTAGTCAGTCT
PRIMER_LEFT_1=2,18
PRIMER_RIGHT_1=56,18
PRIMER_INTERNAL_1=20,18
PRIMER_LEFT_1_TM=52.936
PRIMER_RIGHT_1_TM=41.431
PRIMER_INTERNAL_1_TM=43.690
PRIMER_LEFT_1_GC_PERCENT=55.556
PRIMER_RIGHT_1_GC_PERCENT=44.444
PRIMER_INTERNAL_1_GC_PERCENT=44.444
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_MIN_SEQ_QUALITY=30
PRIMER_RIGHT_1_MIN_SEQ_QUALITY=20
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_INTERNAL_1_MIN_SEQ_QUALITY=50
PRIMER_PAIR_1_COMPL_ANY=10.00
PRIMER_PAIR_1_COMPL_END=9.00
PRIMER_PAIR_1_PRODUCT_SIZE=55
PRIMER_PAIR_1_PRODUCT_TM=68.4
PRIMER_PAIR_2_PENALTY=20.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_INTERNAL_2_PENALTY=20.000000
PRIMER_LEFT_2_SEQUENCE=CGTAGCTGATCGTAGCTG
PRIMER_RIGHT_2_SEQUENCE=GATCAGCTAGATCAGTAGT
PRIMER_INTERNAL_2_SEQUENCE=ATCGTAGCTAGTCAGTCT
PRIMER_LEFT_2=2,18
PRIMER_RIGHT_2=56,19
PRIMER_INTERNAL_2=20,18
PRIMER_LEFT_2_TM=52.936
PRIMER_RIGHT_2_TM=43.262
PRIMER_INTERNAL_2_TM=43.690
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=42.105
PRIMER_INTERNAL_2_GC_PERCENT=44.444
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_MIN_SEQ_QUALITY=30
PRIMER_RIGHT_2_MIN_SEQ_QUALITY=20
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.1000
PRIMER_INTERNAL_2_MIN_SEQ_QUALITY=50
PRIMER_PAIR_2_COMPL_ANY=10.00
PRIMER_PAIR_2_COMPL_END=9.00
PRIMER_PAIR_2_PRODUCT_SIZE=55
PRIMER_PAIR_2_PRODUCT_TM=68.4
PRIMER_PAIR_3_PENALTY=20.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_INTERNAL_3_PENALTY=20.000000
PRIMER_LEFT_3_SEQUENCE=CGTAGCTGATCGTAGCTG
PRIMER_RIGHT_3_SEQUENCE=TGATCAGCTAGATCAGTA
PRIMER_INTERNAL_3_SEQUENCE=ATCGTAGCTAGTCAGTCT
PRIMER_LEFT_3=2,18
PRIMER_RIGHT_3=57,18
PRIMER_INTERNAL_3=20,18
PRIMER_LEFT_3_TM=52.936
PRIMER_RIGHT_3_TM=43.313
PRIMER_INTERNAL_3_TM=43.690
PRIMER_LEFT_3_GC_PERCENT=55.556
PRIMER_RIGHT_3_GC_PERCENT=38.889
PRIMER_INTERNAL_3_GC_PERCENT=44.444
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=10.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_MIN_SEQ_QUALITY=30
PRIMER_RIGHT_3_MIN_SEQ_QUALITY=20
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_INTERNAL_3_MIN_SEQ_QUALITY=50
PRIMER_PAIR_3_COMPL_ANY=10.00
PRIMER_PAIR_3_COMPL_END=10.00
PRIMER_PAIR_3_PRODUCT_SIZE=56
PRIMER_PAIR_3_PRODUCT_TM=68.2
PRIMER_PAIR_4_PENALTY=20.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_INTERNAL_4_PENALTY=20.000000
PRIMER_LEFT_4_SEQUENCE=CGTAGCTGATCGTAGCTG
PRIMER_RIGHT_4_SEQUENCE=TGATCAGCTAGATCAGTAG
PRIMER_INTERNAL_4_SEQUENCE=ATCGTAGCTAGTCAGTCT
PRIMER_LEFT_4=2,18
PRIMER_RIGHT_4=57,19
PRIMER_INTERNAL_4=20,18
PRIMER_LEFT_4_TM=52.936
PRIMER_RIGHT_4_TM=45.394
PRIMER_INTERNAL_4_TM=43.690
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=42.105
PRIMER_INTERNAL_4_GC_PERCENT=44.444
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=10.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_MIN_SEQ_QUALITY=30
PRIMER_RIGHT_4_MIN_SEQ_QUALITY=20
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_INTERNAL_4_MIN_SEQ_QUALITY=50
PRIMER_PAIR_4_COMPL_ANY=10.00
PRIMER_PAIR_4_COMPL_END=9.00
PRIMER_PAIR_4_PRODUCT_SIZE=56
PRIMER_PAIR_4_PRODUCT_TM=68.2
=
SEQUENCE_ID=io_gc_content
PRIMER_WT_SEQ_QUAL=0
PRIMER_INTERNAL_WT_SEQ_QUAL=0
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_INTERNAL_MIN_TM=0
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_GC=40
PRIMER_INTERNAL_OPT_GC_PERCENT=50
PRIMER_INTERNAL_MAX_GC=60
PRIMER_INTERNAL_WT_GC_PERCENT_GT=1
PRIMER_INTERNAL_WT_GC_PERCENT_LT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PAIR_WT_IO_PENALTY=1
PRIMER_PAIR_WT_PR_PENALTY=0
PRIMER_WT_TM_GT=0
PRIMER_WT_TM_LT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_SIZE_GT=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_WT_TM_GT=0
PRIMER_INTERNAL_WT_TM_LT=0
PRIMER_INTERNAL_WT_SIZE_LT=0
PRIMER_INTERNAL_WT_SIZE_GT=0
PRIMER_INTERNAL_WT_SELF_ANY=0
PRIMER_INTERNAL_WT_SELF_END=0
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
PRIMER_LEFT_EXPLAIN=considered 1290, ok 1290
PRIMER_RIGHT_EXPLAIN=considered 1290, ok 1290
PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 1015, ok 550
PRIMER_PAIR_EXPLAIN=considered 522, tm diff too large 2, no internal oligo 463, ok 57
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_INTERNAL_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=TCGGGCCTCTCCCTCAAC
PRIMER_RIGHT_0_SEQUENCE=CCGGCGCAGGAGCCACCT
PRIMER_INTERNAL_0_SEQUENCE=TCACCATGATGACCGTCA
PRIMER_LEFT_0=78,18
PRIMER_RIGHT_0=177,18
PRIMER_INTERNAL_0=96,18
PRIMER_LEFT_0_TM=63.720
PRIMER_RIGHT_0_TM=71.517
PRIMER_INTERNAL_0_TM=57.693
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=77.778
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_0_PRODUCT_TM=82.6
PRIMER_PAIR_1_PENALTY=0.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_INTERNAL_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=CTCGGGCCTCTCCCTCAA
PRIMER_RIGHT_1_SEQUENCE=CCGGCGCAGGAGCCACCT
PRIMER_INTERNAL_1_SEQUENCE=TCACCATGATGACCGTCA
PRIMER_LEFT_1=77,18
PRIMER_RIGHT_1=177,18
PRIMER_INTERNAL_1=96,18
PRIMER_LEFT_1_TM=63.831
PRIMER_RIGHT_1_TM=71.517
PRIMER_INTERNAL_1_TM=57.693
PRIMER_LEFT_1_GC_PERCENT=66.667
PRIMER_RIGHT_1_GC_PERCENT=77.778
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=101
PRIMER_PAIR_1_PRODUCT_TM=82.8
PRIMER_PAIR_2_PENALTY=0.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_INTERNAL_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=CTCGGGCCTCTCCCTCAAC
PRIMER_RIGHT_2_SEQUENCE=CCGGCGCAGGAGCCACCT
PRIMER_INTERNAL_2_SEQUENCE=TCACCATGATGACCGTCA
PRIMER_LEFT_2=77,19
PRIMER_RIGHT_2=177,18
PRIMER_INTERNAL_2=96,18
PRIMER_LEFT_2_TM=64.527
PRIMER_RIGHT_2_TM=71.517
PRIMER_INTERNAL_2_TM=57.693
PRIMER_LEFT_2_GC_PERCENT=68.421
PRIMER_RIGHT_2_GC_PERCENT=77.778
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=101
PRIMER_PAIR_2_PRODUCT_TM=82.8
PRIMER_PAIR_3_PENALTY=0.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_INTERNAL_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=CCTCGGGCCTCTCCCTCA
PRIMER_RIGHT_3_SEQUENCE=CCGGCGCAGGAGCCACCT
PRIMER_INTERNAL_3_SEQUENCE=TCACCATGATGACCGTCA
PRIMER_LEFT_3=76,18
PRIMER_RIGHT_3=177,18
PRIMER_INTERNAL_3=96,18
PRIMER_LEFT_3_TM=66.184
PRIMER_RIGHT_3_TM=71.517
PRIMER_INTERNAL_3_TM=57.693
PRIMER_LEFT_3_GC_PERCENT=72.222
PRIMER_RIGHT_3_GC_PERCENT=77.778
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=102
PRIMER_PAIR_3_PRODUCT_TM=83.0
PRIMER_PAIR_4_PENALTY=0.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_INTERNAL_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=CCTCGGGCCTCTCCCTCAA
PRIMER_RIGHT_4_SEQUENCE=CCGGCGCAGGAGCCACCT
PRIMER_INTERNAL_4_SEQUENCE=TCACCATGATGACCGTCA
PRIMER_LEFT_4=76,19
PRIMER_RIGHT_4=177,18
PRIMER_INTERNAL_4=96,18
PRIMER_LEFT_4_TM=67.190
PRIMER_RIGHT_4_TM=71.517
PRIMER_INTERNAL_4_TM=57.693
PRIMER_LEFT_4_GC_PERCENT=68.421
PRIMER_RIGHT_4_GC_PERCENT=77.778
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=102
PRIMER_PAIR_4_PRODUCT_TM=83.0
=
SEQUENCE_ID=primer_gc_content
PRIMER_WT_SEQ_QUAL=0
PRIMER_INTERNAL_WT_SEQ_QUAL=0
PRIMER_PRODUCT_SIZE_RANGE=50-500
PRIMER_TASK=pick_pcr_primers_and_hyb_probe
PRIMER_INTERNAL_MIN_TM=0
PRIMER_INTERNAL_MAX_TM=100
PRIMER_MIN_GC=40
PRIMER_OPT_GC_PERCENT=50
PRIMER_MAX_GC=60
PRIMER_WT_GC_PERCENT_GT=1
PRIMER_WT_GC_PERCENT_LT=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PAIR_WT_PR_PENALTY=1
PRIMER_WT_TM_GT=0
PRIMER_WT_TM_LT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_SIZE_GT=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_WT_TM_GT=0
PRIMER_INTERNAL_WT_TM_LT=0
PRIMER_INTERNAL_WT_SIZE_LT=0
PRIMER_INTERNAL_WT_SIZE_GT=0
PRIMER_INTERNAL_WT_SELF_ANY=0
PRIMER_INTERNAL_WT_SELF_END=0
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 740, ok 550
PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 932, ok 358
PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 1015, ok 550
PRIMER_PAIR_EXPLAIN=considered 50, unacceptable product size 30, no internal oligo 15, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.000000
PRIMER_LEFT_0_PENALTY=0.000000
PRIMER_RIGHT_0_PENALTY=0.000000
PRIMER_INTERNAL_0_PENALTY=0.000000
PRIMER_LEFT_0_SEQUENCE=ACTTCACTTTCCGACCCT
PRIMER_RIGHT_0_SEQUENCE=CTGACGGTCATCATGGTGAGTT
PRIMER_INTERNAL_0_SEQUENCE=TTTCACCCACGAGTGAAGACTC
PRIMER_LEFT_0=12,18
PRIMER_RIGHT_0=114,22
PRIMER_INTERNAL_0=41,22
PRIMER_LEFT_0_TM=54.516
PRIMER_RIGHT_0_TM=62.314
PRIMER_INTERNAL_0_TM=61.614
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=103
PRIMER_PAIR_0_PRODUCT_TM=78.8
PRIMER_PAIR_1_PENALTY=0.000000
PRIMER_LEFT_1_PENALTY=0.000000
PRIMER_RIGHT_1_PENALTY=0.000000
PRIMER_INTERNAL_1_PENALTY=0.000000
PRIMER_LEFT_1_SEQUENCE=TGACTTCACTTTCCGACC
PRIMER_RIGHT_1_SEQUENCE=CTGACGGTCATCATGGTGAGTT
PRIMER_INTERNAL_1_SEQUENCE=TTTCACCCACGAGTGAAGACTC
PRIMER_LEFT_1=10,18
PRIMER_RIGHT_1=114,22
PRIMER_INTERNAL_1=41,22
PRIMER_LEFT_1_TM=54.341
PRIMER_RIGHT_1_TM=62.314
PRIMER_INTERNAL_1_TM=61.614
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=105
PRIMER_PAIR_1_PRODUCT_TM=78.8
PRIMER_PAIR_2_PENALTY=0.000000
PRIMER_LEFT_2_PENALTY=0.000000
PRIMER_RIGHT_2_PENALTY=0.000000
PRIMER_INTERNAL_2_PENALTY=0.000000
PRIMER_LEFT_2_SEQUENCE=TGACTTCACTTTCCGACCCT
PRIMER_RIGHT_2_SEQUENCE=CTGACGGTCATCATGGTGAGTT
PRIMER_INTERNAL_2_SEQUENCE=TTTCACCCACGAGTGAAGACTC
PRIMER_LEFT_2=10,20
PRIMER_RIGHT_2=114,22
PRIMER_INTERNAL_2=41,22
PRIMER_LEFT_2_TM=59.697
PRIMER_RIGHT_2_TM=62.314
PRIMER_INTERNAL_2_TM=61.614
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=105
PRIMER_PAIR_2_PRODUCT_TM=78.8
PRIMER_PAIR_3_PENALTY=0.000000
PRIMER_LEFT_3_PENALTY=0.000000
PRIMER_RIGHT_3_PENALTY=0.000000
PRIMER_INTERNAL_3_PENALTY=0.000000
PRIMER_LEFT_3_SEQUENCE=ATGACTTCACTTTCCGACCC
PRIMER_RIGHT_3_SEQUENCE=CTGACGGTCATCATGGTGAGTT
PRIMER_INTERNAL_3_SEQUENCE=TTTCACCCACGAGTGAAGACTC
PRIMER_LEFT_3=9,20
PRIMER_RIGHT_3=114,22
PRIMER_INTERNAL_3=41,22
PRIMER_LEFT_3_TM=58.992
PRIMER_RIGHT_3_TM=62.314
PRIMER_INTERNAL_3_TM=61.614
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=106
PRIMER_PAIR_3_PRODUCT_TM=78.6
PRIMER_PAIR_4_PENALTY=0.000000
PRIMER_LEFT_4_PENALTY=0.000000
PRIMER_RIGHT_4_PENALTY=0.000000
PRIMER_INTERNAL_4_PENALTY=0.000000
PRIMER_LEFT_4_SEQUENCE=ATGACTTCACTTTCCGACCCTG
PRIMER_RIGHT_4_SEQUENCE=CTGACGGTCATCATGGTGAGTT
PRIMER_INTERNAL_4_SEQUENCE=TTTCACCCACGAGTGAAGACTC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=114,22
PRIMER_INTERNAL_4=41,22
PRIMER_LEFT_4_TM=62.662
PRIMER_RIGHT_4_TM=62.314
PRIMER_INTERNAL_4_TM=61.614
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=106
PRIMER_PAIR_4_PRODUCT_TM=78.6
=
