PRIMER PICKING RESULTS FOR Check left only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

    0 gtcctcgaacagctcagtccNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
      >>>>>>>>>>>>>>>>>>>>                                        

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 NNNNNNNNNNNNNNNNNNNN
                          

KEYS (in order of precedence):
>>>>>> left primer




PRIMER PICKING RESULTS FOR Check internal only

No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
INTERNAL_OLIGO      90   20   60.19   50.00  2.00  0.00 ccacggatgaggaagaagaa
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

    0 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNccacggatgaggaagaagaaNNNNNNNNNN
                                    ^^^^^^^^^^^^^^^^^^^^          

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 NNNNNNNNNNNNNNNNNNNN
                          

KEYS (in order of precedence):
^^^^^^ internal oligo




PRIMER PICKING RESULTS FOR Check right only

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
RIGHT_PRIMER       199   20   60.01   40.00  2.00  0.00 gcgtttttattgccgttgtt
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

    0 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 aacaacggcaataaaaacgc
      <<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
<<<<<< right primer




PRIMER PICKING RESULTS FOR Pick the same primers as before

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER        198   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
RIGHT PRIMER       315   20   60.01   40.00  2.00  0.00 gcgtttttattgccgttgtt
INTERNAL OLIGO     250   20   60.19   50.00  2.00  0.00 ccacggatgaggaagaagaa
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        >>>>>>>>>>>>>>>>>>>>                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                ^^^^^^^^^^^^^^^^^^^^                          <<<<

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      <<<<<<<<<<<<<<<<                                            

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo



PRIMER PICKING RESULTS FOR Check primers with sequence

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER        198   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
RIGHT PRIMER       315   20   60.01   40.00  2.00  0.00 gcgtttttattgccgttgtt
INTERNAL OLIGO     250   20   60.19   50.00  2.00  0.00 ccacggatgaggaagaagaa
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        >>>>>>>>>>>>>>>>>>>>                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                ^^^^^^^^^^^^^^^^^^^^                          <<<<

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      <<<<<<<<<<<<<<<<                                            

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo



PRIMER PICKING RESULTS FOR Check primers without sequence

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
RIGHT PRIMER       199   20   60.01   40.00  2.00  0.00 gcgtttttattgccgttgtt
INTERNAL OLIGO      90   20   60.19   50.00  2.00  0.00 ccacggatgaggaagaagaa
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 gtcctcgaacagctcagtccNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
      >>>>>>>>>>>>>>>>>>>>                                        

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNccacggatgaggaagaagaaNNNNNNNNNN
                                    ^^^^^^^^^^^^^^^^^^^^          

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 aacaacggcaataaaaacgc
      <<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo



PRIMER PICKING RESULTS FOR Check primers without sequence and internal

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          0   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
RIGHT PRIMER       199   20   60.01   40.00  2.00  0.00 gcgtttttattgccgttgtt
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 gtcctcgaacagctcagtccNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
      >>>>>>>>>>>>>>>>>>>>                                        

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 aacaacggcaataaaaacgc
      <<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer



PRIMER PICKING RESULTS FOR Pick three times the same primers

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527


                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER       1471   20   60.00   50.00  4.00  0.00 gacgtttgctttctgcttcc
 2 LEFT_PRIMER        849   20   60.00   50.00  4.00  0.00 atcgccaccgtacaagaaac
 3 LEFT_PRIMER        296   20   60.01   40.00  2.00  2.00 aacaacggcaataaaaacgc
 4 LEFT_PRIMER        198   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
 5 LEFT_PRIMER         39   20   59.99   55.00  4.00  0.00 ttctctggacacagctccct

                     start  len      tm     gc%   any    3' seq
 1 INTERNAL_OLIGO    1471   20   60.00   50.00  4.00  0.00 gacgtttgctttctgcttcc
 2 INTERNAL_OLIGO     849   20   60.00   50.00  4.00  0.00 atcgccaccgtacaagaaac
 3 INTERNAL_OLIGO     296   20   60.01   40.00  2.00  2.00 aacaacggcaataaaaacgc
 4 INTERNAL_OLIGO     198   20   59.99   60.00  4.00  2.00 gtcctcgaacagctcagtcc
 5 INTERNAL_OLIGO      39   20   59.99   55.00  4.00  0.00 ttctctggacacagctccct

                     start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER      1490   20   60.00   50.00  4.00  2.00 ggaagcagaaagcaaacgtc
 2 RIGHT_PRIMER       868   20   60.00   50.00  4.00  2.00 gtttcttgtacggtggcgat
 3 RIGHT_PRIMER       315   20   60.01   40.00  2.00  0.00 gcgtttttattgccgttgtt
 4 RIGHT_PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
 5 RIGHT_PRIMER        58   20   59.99   55.00  4.00  0.00 agggagctgtgtccagagaa


PRIMER PICKING RESULTS FOR Make a list of a small range

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
SEQUENCE SIZE: 32
INCLUDED REGION SIZE: 32


                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER          3   20   58.72   45.00  4.00  2.00 gatttgatggacatggatgc
 2 LEFT_PRIMER          1   20   58.56   40.00  4.00  2.00 tggatttgatggacatggat
 3 LEFT_PRIMER          0   20   58.56   40.00  4.00  2.00 atggatttgatggacatgga
 4 LEFT_PRIMER          0   21   58.93   38.10  4.00  2.00 atggatttgatggacatggat
 5 LEFT_PRIMER          1   19   58.16   42.11  4.00  2.00 tggatttgatggacatgga
 6 LEFT_PRIMER          2   21   62.41   47.62  4.00  2.00 ggatttgatggacatggatgc

                     start  len      tm     gc%   any    3' seq
 1 INTERNAL_OLIGO      12   20   60.30   45.00 11.00  9.00 gacatggatgccatcaatga
 2 INTERNAL_OLIGO       4   20   60.56   45.00  4.00  4.00 atttgatggacatggatgcc
 3 INTERNAL_OLIGO       3   20   58.72   45.00  4.00  2.00 gatttgatggacatggatgc
 4 INTERNAL_OLIGO       5   19   60.28   47.37  4.00  4.00 tttgatggacatggatgcc
 5 INTERNAL_OLIGO       8   19   59.67   52.63 10.00 10.00 gatggacatggatgccatc
 6 INTERNAL_OLIGO       2   20   58.56   45.00  4.00  4.00 ggatttgatggacatggatg
 7 INTERNAL_OLIGO       1   20   58.56   40.00  4.00  2.00 tggatttgatggacatggat
 8 INTERNAL_OLIGO       0   20   58.56   40.00  4.00  2.00 atggatttgatggacatgga
 9 INTERNAL_OLIGO       9   19   60.74   47.37  8.00  5.00 atggacatggatgccatca
10 INTERNAL_OLIGO       7   19   60.74   47.37  8.00  8.00 tgatggacatggatgccat
11 INTERNAL_OLIGO      11   19   59.11   47.37  9.00  9.00 ggacatggatgccatcaat
12 INTERNAL_OLIGO       0   21   58.93   38.10  4.00  2.00 atggatttgatggacatggat
13 INTERNAL_OLIGO      11   20   62.13   50.00 11.00 11.00 ggacatggatgccatcaatg
14 INTERNAL_OLIGO      10   20   62.13   45.00  9.00  9.00 tggacatggatgccatcaat
15 INTERNAL_OLIGO       9   20   62.13   45.00  8.00  7.00 atggacatggatgccatcaa
16 INTERNAL_OLIGO       6   20   62.13   45.00  8.00  8.00 ttgatggacatggatgccat
17 INTERNAL_OLIGO      10   18   60.45   50.00  7.00  5.00 tggacatggatgccatca
18 INTERNAL_OLIGO       7   18   60.45   50.00  6.00  6.00 tgatggacatggatgcca
19 INTERNAL_OLIGO       1   21   61.53   42.86  4.00  4.00 tggatttgatggacatggatg
20 INTERNAL_OLIGO       8   20   62.76   50.00 10.00  6.00 gatggacatggatgccatca

                     start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER        23   20   60.56   45.00  4.00  2.00 ggcatccatgtccatcaaat
 2 RIGHT_PRIMER        22   20   58.72   45.00  4.00  0.00 gcatccatgtccatcaaatc
 3 RIGHT_PRIMER        23   19   60.28   47.37  4.00  0.00 ggcatccatgtccatcaaa
 4 RIGHT_PRIMER        21   20   58.56   45.00  4.00  0.00 catccatgtccatcaaatcc
 5 RIGHT_PRIMER        20   20   58.56   40.00  4.00  0.00 atccatgtccatcaaatcca
 6 RIGHT_PRIMER        19   20   58.56   40.00  4.00  2.00 tccatgtccatcaaatccat
 7 RIGHT_PRIMER        25   19   60.74   47.37  8.00  1.00 atggcatccatgtccatca
 8 RIGHT_PRIMER        20   21   58.93   38.10  4.00  2.00 atccatgtccatcaaatccat
 9 RIGHT_PRIMER        25   20   62.13   45.00  8.00  2.00 atggcatccatgtccatcaa
10 RIGHT_PRIMER        24   18   60.45   50.00  6.00  1.00 tggcatccatgtccatca
11 RIGHT_PRIMER        21   21   61.53   42.86  4.00  0.00 catccatgtccatcaaatcca
12 RIGHT_PRIMER        19   19   58.16   42.11  4.00  0.00 tccatgtccatcaaatcca
13 RIGHT_PRIMER        24   19   61.95   47.37  6.00  2.00 tggcatccatgtccatcaa
14 RIGHT_PRIMER        23   18   58.72   50.00  4.00  2.00 ggcatccatgtccatcaa
15 RIGHT_PRIMER        23   21   62.41   47.62  4.00  0.00 ggcatccatgtccatcaaatc
16 RIGHT_PRIMER        22   21   62.41   47.62  4.00  0.00 gcatccatgtccatcaaatcc
17 RIGHT_PRIMER        21   22   61.72   40.91  4.00  2.00 catccatgtccatcaaatccat


PRIMER PICKING RESULTS FOR Make a list of a small range

No mispriming library specified
Using 0-based sequence positions
SEQUENCE SIZE: 32
INCLUDED REGION SIZE: 32


                    start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER        23   20   60.56   45.00  4.00  2.00 ggcatccatgtccatcaaat
 2 RIGHT_PRIMER        22   20   58.72   45.00  4.00  0.00 gcatccatgtccatcaaatc


PRIMER PICKING RESULTS FOR Check left only too small

No mispriming library specified
Using 0-based sequence positions
WARNING: Specified left primer < PRIMER_MIN_SIZE; Left primer is unacceptable: Too short/Tm too low

OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   11   27.26   54.55  4.00  2.00 gtcctcgaaca
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

    0 gtcctcgaacaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
      >>>>>>>>>>>                                                 

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 NNNNNNNNNNNNNNNNNNNN
                          

KEYS (in order of precedence):
>>>>>> left primer




PRIMER PICKING RESULTS FOR Check left only too big

No mispriming library specified
Using 0-based sequence positions
WARNING: Specified left primer > PRIMER_MAX_SIZE; Left primer is unacceptable: Too long/Tm too high

OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER          0   36   80.81   55.56  4.00  0.00 tgacctccacttctctggacacagctccctcaccaa
SEQUENCE SIZE: 200
INCLUDED REGION SIZE: 200

    0 tgacctccacttctctggacacagctccctcaccaaNNNNNNNNNNNNNNNNNNNNNNNN
      >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>                        

   60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                                                                  

  180 NNNNNNNNNNNNNNNNNNNN
                          

KEYS (in order of precedence):
>>>>>> left primer




PRIMER PICKING RESULTS FOR Pick sequencing primers no target

No mispriming library specified
Using 0-based sequence positions
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

TARGETS (start, len)*: 0,1527
    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
      *******************>>>>>>>>>>>>>>>>>>>**********************

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
      ************************************************************

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
      ************************************************************

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
      ******************<<<<<<<<<<<<<<<<<<<<**********************

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
      ************************************************************

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      ************>>>>>>>>>>>>>>>>>>>>****************************

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
      ************************************************************

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
      ************************************************************

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      ************************************************************

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
      ************************************************************

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      ************************************************************

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
      ******************<<<<<<<<<<<<<<<<<<<<**********************

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
      ************************************************************

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
      *********************>>>>>>>>>>>>>>>>>>>>*******************

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
      ************************************************************

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
      ************************************************************

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
      ************************************************************

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
      ************************************************************

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
      ************************************************************

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
      ********************************************<<<<<<<<<<<<<<<<

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
      <<<<********************************************************

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
      *********************************************************>>>

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
      >>>>>>>>>>>>>>>>>*******************************************

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
      ************************************************************

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
      *************************************************<<<<<<<<<<<

 1500 tgttcttcttcagattctgatttatag
      <<<<<<<<<<<****************

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ left primer / right primer overlap


                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER         19   19   59.87   47.37  5.00  0.00 atgccatcaatgacctcca
 2 LEFT_PRIMER        312   20   60.12   45.00  4.00  2.00 acgcatgaagttggtgatga
 3 LEFT_PRIMER        801   20   59.92   45.00  6.00  2.00 catcttcggataaccgcatt
 4 LEFT_PRIMER       1317   20   60.23   60.00  2.00  0.00 ggggagtggttaggtgaggt

                     start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
 2 RIGHT_PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
 3 RIGHT_PRIMER      1203   20   60.05   45.00  3.00  3.00 gaaaaccagcctccaatgaa
 4 RIGHT_PRIMER      1510   22   59.26   45.45  2.00  0.00 gaagaagaacaagtccaaaggg


PRIMER PICKING RESULTS FOR Pick sequencing primers small target

No mispriming library specified
Using 0-based sequence positions
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

TARGETS (start, len)*: 450,37
    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                                                                  

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                  >>>>>>>>>>>>>>>>

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      >>>>                                                        

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                    ******************************

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      *******                                                     

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                         <<<<<<<<<

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
      <<<<<<<<<<<                                                 

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ left primer / right primer overlap


                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER        284   20   59.36   45.00  2.00  1.00 aaccacaacaacaacaacgg

                     start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER       670   20   60.50   45.00  4.00  2.00 aagtggccgaatttgacgta


PRIMER PICKING RESULTS FOR Pick sequencing primers left only

No mispriming library specified
Using 0-based sequence positions
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

TARGETS (start, len)*: 150,800
    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                         >>>>>>>>>>>>>>>>>>>                      

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                    ******************************

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
      ************************************************************

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
      ************************************************************

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      ************************************************************

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
      ************************************************************

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
      *********************************************************>>>

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      >>>>>>>>>>>>>>>>>*******************************************

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
      ************************************************************

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      ************************************************************

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
      ************************************************************

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
      ************************************************************

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
      ************************************************************

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
      ************************************************************

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
      **************************************************          

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ left primer / right primer overlap


                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER         19   19   59.87   47.37  5.00  0.00 atgccatcaatgacctcca
 2 LEFT_PRIMER        477   20   60.41   50.00  3.00  0.00 agacttgccgctcatttcac

 

PRIMER PICKING RESULTS FOR Pick sequencing primers right only

No mispriming library specified
Using 0-based sequence positions
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

TARGETS (start, len)*: 150,800
    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                    ******************************

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
      ************************************************************

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
      ************************************************************

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      ************************************************************

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
      ************************************************************

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
      ************************************************************

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      ************************************************************

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
      ************************************************************

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      *********<<<<<<<<<<<<<<<<<<<<*******************************

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
      ************************************************************

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
      ************************************************************

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
      ************************************************************

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
      ************************************************************

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
      **************************************************          

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
              <<<<<<<<<<<<<<<<<<<<                                

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ left primer / right primer overlap


                    start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER       628   20   60.03   50.00  3.00  1.00 tggaaagcagcaagtgtgtc
 2 RIGHT_PRIMER      1107   20   59.34   50.00  4.00  2.00 cttcaaccaacgtcacaagc


PRIMER PICKING RESULTS FOR Pick sequencing primers three target

No mispriming library specified
Using 0-based sequence positions
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

TARGETS (start, len)*: 350,37 450,100 850,500 1200,100
    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                             >>>>>

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
      >>>>>>>>>>>>>>>                                             

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                              >>>>

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      >>>>>>>>>>>>>>>>                                  **********

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
      ***************************                                 

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                    ******************************

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      ************************************************************

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
      ***<<<<<<<<<<<<<<<<<<<<                                     

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
             >>>>>>>>>>>^^^^^^^^^<<<<<<<<<<<                      

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                **************************************************

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
      ************************************************************

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
      ************************************************************

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
      *<<<<<<<<<<<<<<<<<<^^>>>>>>>>>>>>>>>>>>*********************

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
      ************************************************************

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
      ********************************>>>>>>>>>>>>>>>>>>>>********

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
      ************************************************************

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
      ************************************************************

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
      ******************************                              

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                         <<<<<<<<<<<<<<<<<<<<     

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                       <<<<<<<<<<<

 1500 tgttcttcttcagattctgatttatag
      <<<<<<<<<<<                

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer
^^^^^^ left primer / right primer overlap


                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER        175   20   59.63   50.00  6.00  0.00 ccatcatcgaggacagaaca
 2 LEFT_PRIMER        296   20   60.01   40.00  2.00  2.00 aacaacggcaataaaaacgc
 3 LEFT_PRIMER        667   20   60.26   50.00  3.00  1.00 acttcacagccaatcaagcc
 4 LEFT_PRIMER       1172   20   59.94   40.00  5.00  1.00 agcggtttttgattcattgg
 5 LEFT_PRIMER       1039   20   60.25   45.00  6.00  0.00 cggaatcagttgcttcgttt

                     start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER       562   20   59.70   45.00  2.00  0.00 ttgttgtggtgatggtggtt
 2 RIGHT_PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
 3 RIGHT_PRIMER      1040   20   60.05   55.00  6.00  3.00 cggtgcacggtaactaaggt
 4 RIGHT_PRIMER      1510   22   59.26   45.45  2.00  0.00 gaagaagaacaagtccaaaggg
 5 RIGHT_PRIMER      1434   20   59.96   55.00  3.00  2.00 cacccacctcttcaacccta


PRIMER PICKING RESULTS FOR Pick fixed left primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                     >>>>>>>>>>>>>>>>>>>>                         

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        <<<<<<<<<<<<<<<<<<<<                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
   RIGHT PRIMER       125   20   59.97   55.00  3.00  2.00 gttgacgatgggagaccagt
   PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
   RIGHT PRIMER       126   20   60.11   55.00  3.00  1.00 agttgacgatgggagaccag
   PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00


PRIMER PICKING RESULTS FOR Pick fixed right primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER        596   20   60.05   50.00  2.00  0.00 cgacaaccaaaacgacacac
RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                                                                  

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                              >>>>

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      >>>>>>>>>>>>>>>>                                            

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                        <<<<<<<<<<<<<<<<<<<<                      

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER        595   20   60.05   45.00  2.00  0.00 acgacaaccaaaacgacaca
   RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
   PRODUCT SIZE: 103, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        594   20   60.05   50.00  2.00  0.00 cacgacaaccaaaacgacac
   RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
   PRODUCT SIZE: 104, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00


PRIMER PICKING RESULTS FOR Pick start fixed left primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                     >>>>>>>>>>>>>>>>>>>>                         

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        <<<<<<<<<<<<<<<<<<<<                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
   RIGHT PRIMER       125   20   59.97   55.00  3.00  2.00 gttgacgatgggagaccagt
   PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
   RIGHT PRIMER       126   20   60.11   55.00  3.00  1.00 agttgacgatgggagaccag
   PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00


PRIMER PICKING RESULTS FOR Pick start fixed right primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER        596   20   60.05   50.00  2.00  0.00 cgacaaccaaaacgacacac
RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                                                                  

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                              >>>>

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      >>>>>>>>>>>>>>>>                                            

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                        <<<<<<<<<<<<<<<<<<<<                      

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER        595   20   60.05   45.00  2.00  0.00 acgacaaccaaaacgacaca
   RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
   PRODUCT SIZE: 103, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        594   20   60.05   50.00  2.00  0.00 cacgacaaccaaaacgacac
   RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
   PRODUCT SIZE: 104, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00


PRIMER PICKING RESULTS FOR Pick end fixed left primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                     >>>>>>>>>>>>>>>>>>>>                         

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        <<<<<<<<<<<<<<<<<<<<                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
   RIGHT PRIMER       125   20   59.97   55.00  3.00  2.00 gttgacgatgggagaccagt
   PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00


PRIMER PICKING RESULTS FOR Pick end fixed right primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER        596   20   60.05   50.00  2.00  0.00 cgacaaccaaaacgacacac
RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                                                                  

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                              >>>>

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      >>>>>>>>>>>>>>>>                                            

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                        <<<<<<<<<<<<<<<<<<<<                      

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER        595   20   60.05   45.00  2.00  0.00 acgacaaccaaaacgacaca
   RIGHT PRIMER       697   20   60.19   45.00  4.00  1.00 gcttcgatgatggcttgatt
   PRODUCT SIZE: 103, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00


PRIMER PICKING RESULTS FOR Pick cloning primers

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 203

PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
      ...............>>>>>>>>>>>>>>>>>>>>                         

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        <<<<<<<<<<<<<<<<<<<<......................

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
      ............................................................

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      ............................................................

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
      ............................................................

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
      ............................................................

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      ............................................................

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
      ............................................................

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      ............................................................

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
      ............................................................

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
      ............................................................

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
      ............................................................

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
      ............................................................

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
      ............................................................

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
      ............................................................

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
      ............................................................

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
      ............................................................

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
      ............................................................

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
      ............................................................

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
      ............................................................

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
      ............................................................

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
      ............................................................

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
      ............................................................

 1500 tgttcttcttcagattctgatttatag
      ...........................

KEYS (in order of precedence):
...... vector sequence
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         15   19   59.11   47.37  9.00  2.00 atggatgccatcaatgacc
   RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
   PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00


PRIMER PICKING RESULTS FOR Pick cloning primers with only right primer

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
RIGHT_PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 203

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
      ...............                                             

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        <<<<<<<<<<<<<<<<<<<<......................

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
      ............................................................

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
      ............................................................

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
      ............................................................

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
      ............................................................

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
      ............................................................

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
      ............................................................

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
      ............................................................

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
      ............................................................

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
      ............................................................

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
      ............................................................

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
      ............................................................

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
      ............................................................

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
      ............................................................

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
      ............................................................

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
      ............................................................

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
      ............................................................

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
      ............................................................

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
      ............................................................

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
      ............................................................

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
      ............................................................

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
      ............................................................

 1500 tgttcttcttcagattctgatttatag
      ...........................

KEYS (in order of precedence):
...... vector sequence
<<<<<< right primer


ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER       217   19   59.06   57.89  4.00  2.00 ggactgagctgttcgagga


PRIMER PICKING RESULTS FOR Pick discriminative primers

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 35,163

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                     >>>>>>>>>>>>>>>>>>>>*************************

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
      ************************************************************

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
      ************************************************************

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
      ******************<<<<<<<<<<<<<<<<<<<<                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         16   19   59.87   47.37  7.00  4.00 tggatgccatcaatgacct
   RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
   PRODUCT SIZE: 202, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00


PRIMER PICKING RESULTS FOR Pick start fixed left primer unique

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER         15   20   60.17   45.00  9.00  4.00 atggatgccatcaatgacct
RIGHT PRIMER       217   20   59.99   60.00  4.00  2.00 ggactgagctgttcgaggac
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                     >>>>>>>>>>>>>>>>>>>>                         

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                        <<<<<<<<<<<<<<<<<<<<                      

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                                                  

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER         15   19   59.11   47.37  9.00  2.00 atggatgccatcaatgacc
   RIGHT PRIMER       125   20   59.97   55.00  3.00  2.00 gttgacgatgggagaccagt
   PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER         15   21   62.05   47.62  9.00  6.00 atggatgccatcaatgacctc
   RIGHT PRIMER       126   20   60.11   55.00  3.00  1.00 agttgacgatgggagaccag
   PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00


PRIMER PICKING RESULTS FOR Pick a left primer and an internal oligo

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT_PRIMER       1471   20   60.00   50.00  4.00  0.00 gacgtttgctttctgcttcc
INTERNAL_OLIGO    1471   20   60.00   50.00  4.00  0.00 gacgtttgctttctgcttcc
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                                                                  

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                     >>>>>>>>>>>>>>>>>>>>         

 1500 tgttcttcttcagattctgatttatag
                                 

KEYS (in order of precedence):
>>>>>> left primer


ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq
ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq


PRIMER PICKING RESULTS FOR Pick a right primer and an internal oligo

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
INTERNAL_OLIGO    1471   20   60.00   50.00  4.00  0.00 gacgtttgctttctgcttcc
RIGHT_PRIMER      1490   20   60.00   50.00  4.00  2.00 ggaagcagaaagcaaacgtc
SEQUENCE SIZE: 1527
INCLUDED REGION SIZE: 1527

    0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc
                                                                  

   60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc
                                                                  

  120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc
                                                                  

  180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc
                                                                  

  240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca
                                                                  

  300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta
                                                                  

  360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg
                                                                  

  420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga
                                                                  

  480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat
                                                                  

  540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac
                                                                  

  600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa
                                                                  

  660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc
                                                                  

  720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt
                                                                  

  780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc
                                                                  

  840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc
                                                                  

  900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct
                                                                  

  960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct
                                                                  

 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg
                                                                  

 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg
                                                                  

 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt
                                                                  

 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt
                                                                  

 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg
                                                                  

 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa
                                                                  

 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt
                                                                  

 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact
                                     <<<<<<<<<<<<<<<<<<<<^^^^^^^^^

 1500 tgttcttcttcagattctgatttatag
      ^^^^^^^^^^                 

KEYS (in order of precedence):
<<<<<< right primer


ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq
ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq


PRIMER PICKING RESULTS FOR Test behavior of SEQUENCE_PRIMER_PAIR_OK_REGION_LIST with PRIMER_TASK=pick_primer_list

No mispriming library specified
Using 1-based sequence positions
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,20,49,17

                    start  len      tm     gc%   any    3' seq
 1 LEFT_PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
 2 LEFT_PRIMER          2   19   49.45   26.32 11.00  5.00 ttgattcaatgaatctcaa
 3 LEFT_PRIMER          1   19   48.92   31.58 11.00  7.00 cttgattcaatgaatctca
 4 LEFT_PRIMER          3   18   47.09   27.78 11.00  3.00 tgattcaatgaatctcaa
 5 LEFT_PRIMER          2   18   47.09   27.78 11.00  7.00 ttgattcaatgaatctca
 6 LEFT_PRIMER          1   18   45.15   33.33 11.00  7.00 cttgattcaatgaatctc
 7 LEFT_PRIMER          3   17   44.38   29.41 11.00  5.00 tgattcaatgaatctca
 8 LEFT_PRIMER          4   17   43.02   29.41 11.00  2.00 gattcaatgaatctcaa
 9 LEFT_PRIMER          2   17   43.02   29.41 11.00  7.00 ttgattcaatgaatctc
10 LEFT_PRIMER          1   17   42.26   29.41 11.00  9.00 cttgattcaatgaatct
11 LEFT_PRIMER          4   16   39.89   31.25 11.00  4.00 gattcaatgaatctca
12 LEFT_PRIMER          3   16   39.89   31.25 11.00  7.00 tgattcaatgaatctc
13 LEFT_PRIMER          5   16   39.85   25.00  9.00  2.00 attcaatgaatctcaa
14 LEFT_PRIMER          2   16   39.85   25.00 11.00  9.00 ttgattcaatgaatct
15 LEFT_PRIMER          1   16   39.85   31.25 11.00 11.00 cttgattcaatgaatc

                     start  len      tm     gc%   any    3' seq
 1 RIGHT_PRIMER        65   17   45.95   29.41  2.00  1.00 aaaaaaacacaatccAg
 2 RIGHT_PRIMER        65   16   43.92   25.00  2.00  0.00 aaaaaaacacaatccA
 3 RIGHT_PRIMER        64   16   43.31   31.25  2.00  1.00 aaaaaacacaatccAg

Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left     954     0     0     0   939     0     0     0     0     0     0     0     0    15
Right    954     0     0     0   951     0     0     0     0     0     0     0     0     3
Pair Stats:
considered 0, ok 0
libprimer3 release 2.2.3


PRIMER PICKING RESULTS FOR rev_comp_primers

INPUT PROBLEM: Specified left and right primer are reverse complementary

PRIMER PICKING RESULTS FOR identical_primers

INPUT PROBLEM: Specified left and right primer are identical

