PRIMER_SECONDARY_STRUCTURE_ALIGNMENT=1
PRIMER_FIRST_BASE_INDEX=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=../src/humrep_and_simple.txt
PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 200-299 501-600 100-199 601-700 701-850 851-1001
PRIMER_NUM_RETURN=5
PRIMER_MAX_END_STABILITY=9.0
PRIMER_MAX_LIBRARY_MISPRIMING=12.00
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00
PRIMER_MAX_SELF_ANY_TH=47.00
PRIMER_MAX_SELF_END_TH=47.00
PRIMER_MAX_HAIRPIN_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00
PRIMER_PAIR_MAX_COMPL_END_TH=47.00
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=59.0
PRIMER_MAX_TM=63.0
PRIMER_PAIR_MAX_DIFF_TM=5.0
PRIMER_TM_FORMULA=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_DIVALENT=1.5
PRIMER_DNTP_CONC=0.6
PRIMER_DNA_CONC=50.0
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_LOWERCASE_MASKING=0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_POLY_X=5
PRIMER_OUTSIDE_PENALTY=0
PRIMER_GC_CLAMP=0
PRIMER_LIBERAL_BASE=1
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_WT_TM_LT=1.0
PRIMER_WT_TM_GT=1.0
PRIMER_WT_SIZE_LT=1.0
PRIMER_WT_SIZE_GT=1.0
PRIMER_WT_GC_PERCENT_LT=0.0
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_SELF_ANY_TH=0.0
PRIMER_WT_SELF_END_TH=0.0
PRIMER_WT_HAIRPIN_TH=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_LIBRARY_MISPRIMING=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY_TH=0.0
PRIMER_PAIR_WT_COMPL_END_TH=0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PICK_ANYWAY=0
PRIMER_TASK=generic
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=19
PRIMER_MAX_SIZE=21
SEQUENCE_ID=start_up
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 21, low tm 19, ok 2
PRIMER_RIGHT_EXPLAIN=considered 21, low tm 19, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_NUM_RETURN=1
PRIMER_MAX_SELF_ANY_TH=47.00
PRIMER_MAX_SELF_END_TH=47.00
PRIMER_MAX_HAIRPIN_TH=47.0
PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00
PRIMER_PAIR_MAX_COMPL_END_TH=47.00
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_ID=pick_detection_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 45, too many Ns 3, low tm 28, high repeat similarity 1, ok 13
PRIMER_RIGHT_EXPLAIN=considered 90, low tm 36, high tm 12, ok 42
PRIMER_PAIR_EXPLAIN=considered 18, unacceptable product size 11, high mispriming library similarity 4, ok 3
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1.608735
PRIMER_LEFT_0_PENALTY=1.001812
PRIMER_RIGHT_0_PENALTY=0.606923
PRIMER_LEFT_0_SEQUENCE=AGACATGACACTGACGATGC
PRIMER_RIGHT_0_SEQUENCE=AGCTATCGATTTGGGTCGGG
PRIMER_LEFT_0=10,20
PRIMER_RIGHT_0=97,20
PRIMER_LEFT_0_TM=57.998
PRIMER_RIGHT_0_TM=59.607
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=1.71
PRIMER_RIGHT_0_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n      5' AGCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCGA 5'\n
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=41.74
PRIMER_RIGHT_0_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' AGCTATCGATTTU+2510\n         |||   U+2502\n    3' GGGCTGGGU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_0_END_STABILITY=3.9100
PRIMER_RIGHT_0_END_STABILITY=5.1400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=1.96
PRIMER_PAIR_0_COMPL_END_STUCT=Tm: 2.0&deg;C  dG: -1409 cal/mol  dH: -77000 cal/mol  dS: -244 cal/mol*K\n5' AGCTATCGATTTGGGTCGGG 3'\n       ||||    | |||  |\n  3' CGTAGCAGT-CACAGTACAGA 5'\n
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_0_PRODUCT_TM=82.1
PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=11.40
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_detection_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 184, too many Ns 5, GC content failed 1, low tm 140, long poly-x seq 16, ok 22
PRIMER_RIGHT_EXPLAIN=considered 216, low tm 167, high tm 13, ok 36
PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2.181798
PRIMER_LEFT_0_PENALTY=1.695359
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_LEFT_0_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_LEFT_0=53,21
PRIMER_RIGHT_0=137,20
PRIMER_LEFT_0_TM=58.305
PRIMER_RIGHT_0_TM=58.514
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=3.7700
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_PAIR_0_COMPL_ANY_TH=10.83
PRIMER_PAIR_0_COMPL_ANY_STUCT=Tm: 10.8&deg;C  dG: -6304 cal/mol  dH: -53300 cal/mol  dS: -152 cal/mol*K\n5' TGACCTGATAGAAACGCCAAC 3'\n              ||||||\n     3' TAAGGTTTTGCGTTTCGTGC 5'\n
PRIMER_PAIR_0_COMPL_END_TH=1.44
PRIMER_PAIR_0_COMPL_END_STUCT=Tm: 1.4&deg;C  dG: -2915 cal/mol  dH: -64100 cal/mol  dS: -197 cal/mol*K\n5' CGTGCTTTGCGTTTTGGAAT 3'\n           ||||||    ||\n    3' CAACCGCAAAGA--TAGTCCAGT 5'\n
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_0_PRODUCT_TM=78.8
PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=15.19
=
PRIMER_TASK=pick_left_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_left_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.527529
PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC
PRIMER_LEFT_0=61,20
PRIMER_LEFT_0_TM=59.528
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=10.70
PRIMER_LEFT_0_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n     5' TTAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGATT 5'\n
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=40.47
PRIMER_LEFT_0_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' TTAGTGGGCCATU+2510\n        |||    T\n     3' CCCTAGCU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=4.1902
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_left_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_LEFT_0=118,20
PRIMER_LEFT_0_TM=58.514
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
=
PRIMER_TASK=pick_right_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_right_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 17, high repeat similarity 3, ok 34
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.527529
PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA
PRIMER_RIGHT_0=80,20
PRIMER_RIGHT_0_TM=59.528
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=41.64
PRIMER_RIGHT_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n     5' GGGATCGU+2510\n        |||    A\n3' AATCACCCGGTAU+2518\n
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_RIGHT_0_END_STABILITY=2.2400
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_right_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_RIGHT_0=137,20
PRIMER_RIGHT_0_TM=58.514
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
=
PRIMER_TASK=pick_hyb_probe_only
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_hyb_probe_only_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_0=66,20
PRIMER_INTERNAL_0_TM=59.273
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n              5' GGGCCATTCGATCCCGACCC 3'\n                  ||||\n3' CCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n    3' CCCAGCCCU+2518\n
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_hyb_probe_only_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_INTERNAL_0=125,20
PRIMER_INTERNAL_0_TM=59.906
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGA 3'\n     ||   |||  |||   ||\n 3' AGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n          3' AGCCCU+2518\n
=
PRIMER_TASK=pick_primer_list
PRIMER_NUM_RETURN=30
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_primer_list_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=30
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_0=66,20
PRIMER_INTERNAL_0_TM=59.273
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n              5' GGGCCATTCGATCCCGACCC 3'\n                  ||||\n3' CCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n    3' CCCAGCCCU+2518\n
PRIMER_INTERNAL_1_PENALTY=1.416589
PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_1=64,21
PRIMER_INTERNAL_1_TM=59.583
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_SELF_ANY_TH=7.03
PRIMER_INTERNAL_1_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n            5' GTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=41.60
PRIMER_INTERNAL_1_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n       3' CCAGCCCU+2518\n
PRIMER_INTERNAL_2_PENALTY=1.438741
PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_2=67,20
PRIMER_INTERNAL_2_TM=58.561
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.60
PRIMER_INTERNAL_2_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n  3' ACCCAGCCCU+2518\n
PRIMER_INTERNAL_3_PENALTY=1.438741
PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_3=65,20
PRIMER_INTERNAL_3_TM=58.561
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=41.60
PRIMER_INTERNAL_3_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TGGGCCATTCGATU+2510\n           |||  U+2502\n      3' CCAGCCCU+2518\n
PRIMER_INTERNAL_4_PENALTY=1.650306
PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_4=66,21
PRIMER_INTERNAL_4_TM=60.650
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_INTERNAL_4_SELF_ANY_TH=2.18
PRIMER_INTERNAL_4_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n               5' GGGCCATTCGATCCCGACCCA 3'\n                   ||||\n3' ACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=41.60
PRIMER_INTERNAL_4_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n   3' ACCCAGCCCU+2518\n
PRIMER_INTERNAL_5_PENALTY=1.650306
PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_5=65,21
PRIMER_INTERNAL_5_TM=60.650
PRIMER_INTERNAL_5_GC_PERCENT=66.667
PRIMER_INTERNAL_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=41.60
PRIMER_INTERNAL_5_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TGGGCCATTCGATU+2510\n           |||  U+2502\n     3' CCCAGCCCU+2518\n
PRIMER_INTERNAL_6_PENALTY=2.095978
PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_6=67,21
PRIMER_INTERNAL_6_TM=58.904
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=41.60
PRIMER_INTERNAL_6_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n 3' AACCCAGCCCU+2518\n
PRIMER_INTERNAL_7_PENALTY=2.360266
PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_7=63,21
PRIMER_INTERNAL_7_TM=58.640
PRIMER_INTERNAL_7_GC_PERCENT=61.905
PRIMER_INTERNAL_7_SELF_ANY_TH=7.03
PRIMER_INTERNAL_7_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n          5' AGTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=41.60
PRIMER_INTERNAL_7_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' AGTGGGCCATTCGATU+2510\n             |||  U+2502\n         3' CAGCCCU+2518\n
PRIMER_INTERNAL_8_PENALTY=2.456420
PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_8=63,20
PRIMER_INTERNAL_8_TM=57.544
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_INTERNAL_8_SELF_ANY_TH=7.03
PRIMER_INTERNAL_8_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n         5' AGTGGGCCATTCGATCCCGA 3'\n              ||| |||\n3' AGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=39.49
PRIMER_INTERNAL_8_HAIRPIN_STUCT=Tm: 39.5&deg;C  dG: -197 cal/mol  dH: -24700 cal/mol  dS: -79 cal/mol*K\n5' AGTGGGCCATU+2510\n      |||    T\n 3' AGCCCTAGCU+2518\n
PRIMER_INTERNAL_9_PENALTY=2.511372
PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_9=64,20
PRIMER_INTERNAL_9_TM=57.489
PRIMER_INTERNAL_9_GC_PERCENT=65.000
PRIMER_INTERNAL_9_SELF_ANY_TH=7.03
PRIMER_INTERNAL_9_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n           5' GTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=41.60
PRIMER_INTERNAL_9_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n        3' CAGCCCU+2518\n
PRIMER_INTERNAL_10_PENALTY=2.626619
PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_10=63,22
PRIMER_INTERNAL_10_TM=60.627
PRIMER_INTERNAL_10_GC_PERCENT=63.636
PRIMER_INTERNAL_10_SELF_ANY_TH=7.03
PRIMER_INTERNAL_10_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n           5' AGTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.60
PRIMER_INTERNAL_10_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' AGTGGGCCATTCGATU+2510\n             |||  U+2502\n        3' CCAGCCCU+2518\n
PRIMER_INTERNAL_11_PENALTY=2.783921
PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_11=67,22
PRIMER_INTERNAL_11_TM=59.216
PRIMER_INTERNAL_11_GC_PERCENT=59.091
PRIMER_INTERNAL_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_INTERNAL_11_HAIRPIN_TH=41.60
PRIMER_INTERNAL_11_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_INTERNAL_12_PENALTY=2.885177
PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA
PRIMER_INTERNAL_12=66,22
PRIMER_INTERNAL_12_TM=60.885
PRIMER_INTERNAL_12_GC_PERCENT=63.636
PRIMER_INTERNAL_12_SELF_ANY_TH=2.18
PRIMER_INTERNAL_12_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n                5' GGGCCATTCGATCCCGACCCAA 3'\n                    ||||\n3' AACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.60
PRIMER_INTERNAL_12_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n  3' AACCCAGCCCU+2518\n
PRIMER_INTERNAL_13_PENALTY=3.114431
PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC
PRIMER_INTERNAL_13=62,23
PRIMER_INTERNAL_13_TM=60.114
PRIMER_INTERNAL_13_GC_PERCENT=60.870
PRIMER_INTERNAL_13_SELF_ANY_TH=7.03
PRIMER_INTERNAL_13_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n          5' TAGTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_INTERNAL_13_HAIRPIN_TH=41.60
PRIMER_INTERNAL_13_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TAGTGGGCCATTCGATU+2510\n              |||  U+2502\n         3' CCAGCCCU+2518\n
PRIMER_INTERNAL_14_PENALTY=3.510595
PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC
PRIMER_INTERNAL_14=64,22
PRIMER_INTERNAL_14_TM=61.511
PRIMER_INTERNAL_14_GC_PERCENT=68.182
PRIMER_INTERNAL_14_SELF_ANY_TH=7.03
PRIMER_INTERNAL_14_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n             5' GTGGGCCATTCGATCCCGACCC 3'\n                 ||| |||\n3' CCCAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=41.60
PRIMER_INTERNAL_14_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n      3' CCCAGCCCU+2518\n
PRIMER_INTERNAL_15_PENALTY=3.685489
PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT
PRIMER_INTERNAL_15=67,23
PRIMER_INTERNAL_15_TM=59.315
PRIMER_INTERNAL_15_GC_PERCENT=56.522
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=41.60
PRIMER_INTERNAL_15_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' GGCCATTCGATU+2510\n          |||  U+2502\n3' TAAACCCAGCCCU+2518\n
PRIMER_INTERNAL_16_PENALTY=3.807618
PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC
PRIMER_INTERNAL_16=62,22
PRIMER_INTERNAL_16_TM=58.192
PRIMER_INTERNAL_16_GC_PERCENT=59.091
PRIMER_INTERNAL_16_SELF_ANY_TH=7.03
PRIMER_INTERNAL_16_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n         5' TAGTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=41.60
PRIMER_INTERNAL_16_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TAGTGGGCCATTCGATU+2510\n              |||  U+2502\n          3' CAGCCCU+2518\n
PRIMER_INTERNAL_17_PENALTY=3.826170
PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_17=68,21
PRIMER_INTERNAL_17_TM=57.174
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_INTERNAL_17_HAIRPIN_TH=41.60
PRIMER_INTERNAL_17_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' GCCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_INTERNAL_18_PENALTY=3.864865
PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT
PRIMER_INTERNAL_18=37,23
PRIMER_INTERNAL_18_TM=59.135
PRIMER_INTERNAL_18_GC_PERCENT=52.174
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_PENALTY=3.881423
PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA
PRIMER_INTERNAL_19=62,21
PRIMER_INTERNAL_19_TM=57.119
PRIMER_INTERNAL_19_GC_PERCENT=57.143
PRIMER_INTERNAL_19_SELF_ANY_TH=7.03
PRIMER_INTERNAL_19_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n        5' TAGTGGGCCATTCGATCCCGA 3'\n              ||| |||\n3' AGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=39.49
PRIMER_INTERNAL_19_HAIRPIN_STUCT=Tm: 39.5&deg;C  dG: -197 cal/mol  dH: -24700 cal/mol  dS: -79 cal/mol*K\n5' TAGTGGGCCATU+2510\n       |||    T\n  3' AGCCCTAGCU+2518\n
PRIMER_INTERNAL_20_PENALTY=3.936624
PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA
PRIMER_INTERNAL_20=65,22
PRIMER_INTERNAL_20_TM=61.937
PRIMER_INTERNAL_20_GC_PERCENT=63.636
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=3.99
PRIMER_INTERNAL_20_SELF_END_STUCT=Tm: 4.0&deg;C  dG: -782 cal/mol  dH: -87600 cal/mol  dS: -280 cal/mol*K\n5' TGGGCCATTCGATCCCGA----CCCA 3'\n   ||||    |||    |||    ||||\n3' ACCC----AGCCCTAGCTTACCGGGT 5'\n
PRIMER_INTERNAL_20_HAIRPIN_TH=42.67
PRIMER_INTERNAL_20_HAIRPIN_STUCT=Tm: 42.7&deg;C  dG: -788 cal/mol  dH: -43900 cal/mol  dS: -139 cal/mol*K\n5' TGGGCCATTCGATU+2510\n   ||||    |||  U+2502\n    3' ACCCAGCCCU+2518\n
PRIMER_INTERNAL_21_PENALTY=3.956741
PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_INTERNAL_21=67,19
PRIMER_INTERNAL_21_TM=57.043
PRIMER_INTERNAL_21_GC_PERCENT=68.421
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.60
PRIMER_INTERNAL_21_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n   3' CCCAGCCCU+2518\n
PRIMER_INTERNAL_22_PENALTY=3.956741
PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_INTERNAL_22=66,19
PRIMER_INTERNAL_22_TM=57.043
PRIMER_INTERNAL_22_GC_PERCENT=68.421
PRIMER_INTERNAL_22_SELF_ANY_TH=2.18
PRIMER_INTERNAL_22_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n             5' GGGCCATTCGATCCCGACC 3'\n                 ||||\n3' CCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=41.60
PRIMER_INTERNAL_22_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n     3' CCAGCCCU+2518\n
PRIMER_INTERNAL_23_PENALTY=4.001118
PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT
PRIMER_INTERNAL_23=36,24
PRIMER_INTERNAL_23_TM=60.001
PRIMER_INTERNAL_23_GC_PERCENT=54.167
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_24_PENALTY=4.001118
PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG
PRIMER_INTERNAL_24=35,24
PRIMER_INTERNAL_24_TM=60.001
PRIMER_INTERNAL_24_GC_PERCENT=54.167
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_INTERNAL_24_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_PENALTY=4.058186
PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC
PRIMER_INTERNAL_25=67,24
PRIMER_INTERNAL_25_TM=59.942
PRIMER_INTERNAL_25_GC_PERCENT=58.333
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=41.60
PRIMER_INTERNAL_25_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n  5' GGCCATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_INTERNAL_26_PENALTY=4.099823
PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA
PRIMER_INTERNAL_26=66,23
PRIMER_INTERNAL_26_TM=61.100
PRIMER_INTERNAL_26_GC_PERCENT=60.870
PRIMER_INTERNAL_26_SELF_ANY_TH=2.18
PRIMER_INTERNAL_26_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n                 5' GGGCCATTCGATCCCGACCCAAA 3'\n                     ||||\n3' AAACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=41.60
PRIMER_INTERNAL_26_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n 3' AAACCCAGCCCU+2518\n
PRIMER_INTERNAL_27_PENALTY=4.159384
PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT
PRIMER_INTERNAL_27=38,22
PRIMER_INTERNAL_27_TM=57.841
PRIMER_INTERNAL_27_GC_PERCENT=54.545
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_28_PENALTY=4.159384
PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG
PRIMER_INTERNAL_28=37,22
PRIMER_INTERNAL_28_TM=57.841
PRIMER_INTERNAL_28_GC_PERCENT=54.545
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_PENALTY=4.196497
PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG
PRIMER_INTERNAL_29=36,23
PRIMER_INTERNAL_29_TM=58.804
PRIMER_INTERNAL_29_GC_PERCENT=56.522
PRIMER_INTERNAL_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=0.00
=
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_pick_primer_list_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=30
PRIMER_RIGHT_NUM_RETURNED=30
PRIMER_INTERNAL_NUM_RETURNED=30
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_LEFT_0=118,20
PRIMER_RIGHT_0=137,20
PRIMER_INTERNAL_0=125,20
PRIMER_LEFT_0_TM=58.514
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=59.906
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGA 3'\n     ||   |||  |||   ||\n 3' AGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n          3' AGCCCU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_1_PENALTY=1.160593
PRIMER_RIGHT_1_PENALTY=1.160593
PRIMER_INTERNAL_1_PENALTY=0.301069
PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_1=122,19
PRIMER_RIGHT_1=140,19
PRIMER_INTERNAL_1=126,20
PRIMER_LEFT_1_TM=59.161
PRIMER_RIGHT_1_TM=59.161
PRIMER_INTERNAL_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_SELF_ANY_TH=7.82
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n 5' ACGCAAAGCACGCTCC-CGAT 3'\n     ||   |||  |||   ||\n3' TAGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=37.44
PRIMER_INTERNAL_1_HAIRPIN_TH=36.42
PRIMER_RIGHT_1_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n    5' GAGCGTGU+2510\n         ||   C\n3' GTTTTGCGTTTU+2518\n
PRIMER_INTERNAL_1_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n        3' TAGCCCU+2518\n
PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_1_END_STABILITY=5.0300
PRIMER_RIGHT_1_END_STABILITY=2.4400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_2_PENALTY=1.656085
PRIMER_RIGHT_2_PENALTY=1.656085
PRIMER_INTERNAL_2_PENALTY=0.900305
PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA
PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG
PRIMER_LEFT_2=117,21
PRIMER_RIGHT_2=137,21
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=58.344
PRIMER_INTERNAL_2_TM=59.100
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.14
PRIMER_INTERNAL_2_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n            3' GCCCU+2518\n
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=5.3400
PRIMER_RIGHT_2_END_STABILITY=1.7500
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=8.6247
PRIMER_LEFT_3_PENALTY=1.695359
PRIMER_RIGHT_3_PENALTY=1.695359
PRIMER_INTERNAL_3_PENALTY=0.956520
PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC
PRIMER_LEFT_3=53,21
PRIMER_RIGHT_3=73,21
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=58.305
PRIMER_RIGHT_3_TM=58.305
PRIMER_INTERNAL_3_TM=59.043
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=36.42
PRIMER_INTERNAL_3_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n      3' CTAGCCCU+2518\n
PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.7700
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_4_PENALTY=1.831685
PRIMER_RIGHT_4_PENALTY=1.831685
PRIMER_INTERNAL_4_PENALTY=1.018091
PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA
PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT
PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=84,20
PRIMER_INTERNAL_4=125,21
PRIMER_LEFT_4_TM=57.168
PRIMER_RIGHT_4_TM=57.168
PRIMER_INTERNAL_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=57.143
PRIMER_INTERNAL_4_SELF_ANY_TH=7.82
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGAT 3'\n     ||   |||  |||   ||\n3' TAGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=35.45
PRIMER_RIGHT_4_HAIRPIN_TH=31.80
PRIMER_INTERNAL_4_HAIRPIN_TH=36.42
PRIMER_LEFT_4_HAIRPIN_STUCT=Tm: 35.4&deg;C  dG: 103 cal/mol  dH: -20400 cal/mol  dS: -66 cal/mol*K\n5' AACGCCAACTCACTU+2510\n            |||  G\n         3' AGTATU+2518\n
PRIMER_RIGHT_4_HAIRPIN_STUCT=Tm: 31.8&deg;C  dG: 377 cal/mol  dH: -22100 cal/mol  dS: -72 cal/mol*K\n         5' TCATAU+2510\n            |||  C\n3' TTGCGGTTGAGTGAU+2518\n
PRIMER_INTERNAL_4_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n         3' TAGCCCU+2518\n
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=2.1500
PRIMER_RIGHT_4_END_STABILITY=4.8400
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.8143
PRIMER_LEFT_5_PENALTY=1.902705
PRIMER_RIGHT_5_PENALTY=1.902705
PRIMER_INTERNAL_5_PENALTY=1.178696
PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA
PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_5=62,20
PRIMER_RIGHT_5=81,20
PRIMER_INTERNAL_5=124,21
PRIMER_LEFT_5_TM=57.097
PRIMER_RIGHT_5_TM=57.097
PRIMER_INTERNAL_5_TM=60.179
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=57.143
PRIMER_INTERNAL_5_SELF_ANY_TH=7.82
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGA 3'\n      ||   |||  |||   ||\n  3' AGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=36.42
PRIMER_INTERNAL_5_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n           3' AGCCCU+2518\n
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=2.7400
PRIMER_RIGHT_5_END_STABILITY=2.5200
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=29.7752
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_6_PENALTY=1.902705
PRIMER_RIGHT_6_PENALTY=1.902705
PRIMER_INTERNAL_6_PENALTY=1.372876
PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT
PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA
PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_6=61,20
PRIMER_RIGHT_6=80,20
PRIMER_INTERNAL_6=126,21
PRIMER_LEFT_6_TM=57.097
PRIMER_RIGHT_6_TM=57.097
PRIMER_INTERNAL_6_TM=60.373
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=7.82
PRIMER_LEFT_6_SELF_ANY_TH=0.00
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n  5' ACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=36.42
PRIMER_INTERNAL_6_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n       3' CTAGCCCU+2518\n
PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=3.5500
PRIMER_RIGHT_6_END_STABILITY=2.1000
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.0753
PRIMER_LEFT_7_PENALTY=1.950877
PRIMER_RIGHT_7_PENALTY=1.950877
PRIMER_INTERNAL_7_PENALTY=1.393730
PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG
PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA
PRIMER_LEFT_7=119,19
PRIMER_RIGHT_7=137,19
PRIMER_INTERNAL_7=126,19
PRIMER_LEFT_7_TM=58.049
PRIMER_RIGHT_7_TM=58.049
PRIMER_INTERNAL_7_TM=59.606
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_INTERNAL_7_GC_PERCENT=63.158
PRIMER_INTERNAL_7_SELF_ANY_TH=7.82
PRIMER_LEFT_7_SELF_ANY_TH=0.00
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' ACGCAAAGCACGCTCC-CGA 3'\n    ||   |||  |||   ||\n3' AGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=0.00
PRIMER_RIGHT_7_HAIRPIN_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=36.42
PRIMER_INTERNAL_7_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n         3' AGCCCU+2518\n
PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=5.3400
PRIMER_RIGHT_7_END_STABILITY=3.5300
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_8_PENALTY=1.972625
PRIMER_RIGHT_8_PENALTY=1.972625
PRIMER_INTERNAL_8_PENALTY=1.595076
PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA
PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_8=56,21
PRIMER_RIGHT_8=76,21
PRIMER_INTERNAL_8=123,21
PRIMER_LEFT_8_TM=58.027
PRIMER_RIGHT_8_TM=58.027
PRIMER_INTERNAL_8_TM=59.405
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_INTERNAL_8_GC_PERCENT=57.143
PRIMER_INTERNAL_8_SELF_ANY_TH=0.00
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=0.00
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=41.14
PRIMER_INTERNAL_8_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n             3' GCCCU+2518\n
PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_LEFT_8_END_STABILITY=3.4100
PRIMER_RIGHT_8_END_STABILITY=3.8600
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=32.2237
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.8066
PRIMER_LEFT_9_PENALTY=1.973945
PRIMER_RIGHT_9_PENALTY=1.973945
PRIMER_INTERNAL_9_PENALTY=2.007622
PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC
PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT
PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_9=55,21
PRIMER_RIGHT_9=75,21
PRIMER_INTERNAL_9=121,22
PRIMER_LEFT_9_TM=58.026
PRIMER_RIGHT_9_TM=58.026
PRIMER_INTERNAL_9_TM=59.992
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_INTERNAL_9_GC_PERCENT=59.091
PRIMER_INTERNAL_9_SELF_ANY_TH=0.00
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=0.00
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=0.00
PRIMER_RIGHT_9_HAIRPIN_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=0.00
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=3.0100
PRIMER_RIGHT_9_END_STABILITY=4.0000
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=27.4392
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=5.5040
PRIMER_LEFT_10_PENALTY=1.973945
PRIMER_RIGHT_10_PENALTY=1.973945
PRIMER_INTERNAL_10_PENALTY=2.235780
PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT
PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC
PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG
PRIMER_LEFT_10=54,21
PRIMER_RIGHT_10=74,21
PRIMER_INTERNAL_10=125,19
PRIMER_LEFT_10_TM=58.026
PRIMER_RIGHT_10_TM=58.026
PRIMER_INTERNAL_10_TM=58.764
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_INTERNAL_10_GC_PERCENT=63.158
PRIMER_INTERNAL_10_SELF_ANY_TH=0.00
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=0.00
PRIMER_RIGHT_10_HAIRPIN_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.14
PRIMER_INTERNAL_10_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n           3' GCCCU+2518\n
PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=3.1600
PRIMER_RIGHT_10_END_STABILITY=3.8500
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=19.5725
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=9.9844
PRIMER_LEFT_11_PENALTY=2.120321
PRIMER_RIGHT_11_PENALTY=2.120321
PRIMER_INTERNAL_11_PENALTY=2.240074
PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA
PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT
PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_11=64,21
PRIMER_RIGHT_11=84,21
PRIMER_INTERNAL_11=124,22
PRIMER_LEFT_11_TM=57.880
PRIMER_RIGHT_11_TM=57.880
PRIMER_INTERNAL_11_TM=60.240
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_INTERNAL_11_GC_PERCENT=54.545
PRIMER_INTERNAL_11_SELF_ANY_TH=7.82
PRIMER_LEFT_11_SELF_ANY_TH=0.00
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGAT 3'\n      ||   |||  |||   ||\n 3' TAGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=35.45
PRIMER_RIGHT_11_HAIRPIN_TH=31.80
PRIMER_INTERNAL_11_HAIRPIN_TH=36.42
PRIMER_LEFT_11_HAIRPIN_STUCT=Tm: 35.4&deg;C  dG: 103 cal/mol  dH: -20400 cal/mol  dS: -66 cal/mol*K\n5' AAACGCCAACTCACTU+2510\n             |||  G\n          3' AGTATU+2518\n
PRIMER_RIGHT_11_HAIRPIN_STUCT=Tm: 31.8&deg;C  dG: 377 cal/mol  dH: -22100 cal/mol  dS: -72 cal/mol*K\n          5' TCATAU+2510\n             |||  C\n3' TTTGCGGTTGAGTGAU+2518\n
PRIMER_INTERNAL_11_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n          3' TAGCCCU+2518\n
PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=2.1500
PRIMER_RIGHT_11_END_STABILITY=3.6000
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8905
PRIMER_LEFT_12_PENALTY=2.142778
PRIMER_RIGHT_12_PENALTY=2.142778
PRIMER_INTERNAL_12_PENALTY=2.299276
PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_12=119,20
PRIMER_RIGHT_12=138,20
PRIMER_INTERNAL_12=122,22
PRIMER_LEFT_12_TM=61.143
PRIMER_RIGHT_12_TM=61.143
PRIMER_INTERNAL_12_TM=60.299
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=59.091
PRIMER_INTERNAL_12_SELF_ANY_TH=0.00
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=0.00
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.14
PRIMER_INTERNAL_12_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' CAAAACGCAAAGCACGCTU+2510\n                 ||  U+2502\n              3' GCCCU+2518\n
PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=5.3400
PRIMER_RIGHT_12_END_STABILITY=3.5300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_13_PENALTY=2.299089
PRIMER_RIGHT_13_PENALTY=2.299089
PRIMER_INTERNAL_13_PENALTY=2.427391
PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC
PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_13=123,19
PRIMER_RIGHT_13=141,19
PRIMER_INTERNAL_13=123,22
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.299
PRIMER_INTERNAL_13_TM=60.427
PRIMER_LEFT_13_GC_PERCENT=52.632
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_INTERNAL_13_GC_PERCENT=54.545
PRIMER_INTERNAL_13_SELF_ANY_TH=7.82
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=0.00
PRIMER_INTERNAL_13_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGA 3'\n       ||   |||  |||   ||\n   3' AGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=0.00
PRIMER_RIGHT_13_HAIRPIN_TH=37.44
PRIMER_INTERNAL_13_HAIRPIN_TH=36.42
PRIMER_RIGHT_13_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n  5' GGAGCGTGU+2510\n        ||   C\n3' TTTTGCGTTTU+2518\n
PRIMER_INTERNAL_13_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n            3' AGCCCU+2518\n
PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_13_END_STABILITY=4.7000
PRIMER_RIGHT_13_END_STABILITY=2.4300
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_14_PENALTY=2.402628
PRIMER_RIGHT_14_PENALTY=2.402628
PRIMER_INTERNAL_14_PENALTY=2.610811
PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT
PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_14=62,21
PRIMER_RIGHT_14=82,21
PRIMER_INTERNAL_14=125,22
PRIMER_LEFT_14_TM=57.597
PRIMER_RIGHT_14_TM=57.597
PRIMER_INTERNAL_14_TM=60.611
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_INTERNAL_14_GC_PERCENT=59.091
PRIMER_INTERNAL_14_SELF_ANY_TH=7.82
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n 5' AACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=0.00
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=0.00
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=36.42
PRIMER_INTERNAL_14_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n        3' CTAGCCCU+2518\n
PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=2.2900
PRIMER_RIGHT_14_END_STABILITY=2.5200
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=30.4432
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_15_PENALTY=2.402628
PRIMER_RIGHT_15_PENALTY=2.402628
PRIMER_INTERNAL_15_PENALTY=2.740095
PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT
PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT
PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT
PRIMER_LEFT_15=60,21
PRIMER_RIGHT_15=80,21
PRIMER_INTERNAL_15=127,19
PRIMER_LEFT_15_TM=57.597
PRIMER_RIGHT_15_TM=57.597
PRIMER_INTERNAL_15_TM=58.260
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_INTERNAL_15_GC_PERCENT=63.158
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=0.00
PRIMER_RIGHT_15_HAIRPIN_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=36.42
PRIMER_INTERNAL_15_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n       3' TAGCCCU+2518\n
PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=3.5500
PRIMER_RIGHT_15_END_STABILITY=1.9800
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0593
PRIMER_LEFT_16_PENALTY=2.455619
PRIMER_RIGHT_16_PENALTY=2.455619
PRIMER_INTERNAL_16_PENALTY=2.880381
PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG
PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC
PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_16=63,21
PRIMER_RIGHT_16=83,21
PRIMER_INTERNAL_16=120,22
PRIMER_LEFT_16_TM=57.544
PRIMER_RIGHT_16_TM=57.544
PRIMER_INTERNAL_16_TM=59.120
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_INTERNAL_16_GC_PERCENT=54.545
PRIMER_INTERNAL_16_SELF_ANY_TH=0.00
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=0.00
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=0.00
PRIMER_RIGHT_16_HAIRPIN_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=0.00
PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_LEFT_16_END_STABILITY=2.3900
PRIMER_RIGHT_16_END_STABILITY=2.7800
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=25.0463
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8601
PRIMER_LEFT_17_PENALTY=2.539776
PRIMER_RIGHT_17_PENALTY=2.539776
PRIMER_INTERNAL_17_PENALTY=2.939429
PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC
PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_17=122,20
PRIMER_RIGHT_17=141,20
PRIMER_INTERNAL_17=122,21
PRIMER_LEFT_17_TM=61.540
PRIMER_RIGHT_17_TM=61.540
PRIMER_INTERNAL_17_TM=58.061
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_TH=37.44
PRIMER_INTERNAL_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n   5' GGAGCGTGU+2510\n         ||   C\n3' GTTTTGCGTTTU+2518\n
PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_17_END_STABILITY=4.7000
PRIMER_RIGHT_17_END_STABILITY=2.4400
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_18_PENALTY=2.539776
PRIMER_RIGHT_18_PENALTY=2.539776
PRIMER_INTERNAL_18_PENALTY=2.939429
PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_18=121,20
PRIMER_RIGHT_18=140,20
PRIMER_INTERNAL_18=121,21
PRIMER_LEFT_18_TM=61.540
PRIMER_RIGHT_18_TM=61.540
PRIMER_INTERNAL_18_TM=58.061
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_INTERNAL_18_GC_PERCENT=57.143
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_TH=37.44
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n     5' GAGCGTGU+2510\n          ||   C\n3' GGTTTTGCGTTTU+2518\n
PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_18_END_STABILITY=5.0300
PRIMER_RIGHT_18_END_STABILITY=3.2800
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_19_PENALTY=2.587613
PRIMER_RIGHT_19_PENALTY=2.587613
PRIMER_INTERNAL_19_PENALTY=3.476553
PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC
PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_19=120,19
PRIMER_RIGHT_19=138,19
PRIMER_INTERNAL_19=123,23
PRIMER_LEFT_19_TM=60.588
PRIMER_RIGHT_19_TM=60.588
PRIMER_INTERNAL_19_TM=60.477
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_INTERNAL_19_GC_PERCENT=52.174
PRIMER_INTERNAL_19_SELF_ANY_TH=7.82
PRIMER_LEFT_19_SELF_ANY_TH=0.00
PRIMER_RIGHT_19_SELF_ANY_TH=0.00
PRIMER_INTERNAL_19_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGAT 3'\n       ||   |||  |||   ||\n  3' TAGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=36.42
PRIMER_INTERNAL_19_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n           3' TAGCCCU+2518\n
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_19_END_STABILITY=5.3400
PRIMER_RIGHT_19_END_STABILITY=3.5300
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=2.588667
PRIMER_RIGHT_20_PENALTY=2.588667
PRIMER_INTERNAL_20_PENALTY=3.598972
PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT
PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_20=121,19
PRIMER_RIGHT_20=139,19
PRIMER_INTERNAL_20=119,23
PRIMER_LEFT_20_TM=60.589
PRIMER_RIGHT_20_TM=60.589
PRIMER_INTERNAL_20_TM=59.401
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=52.632
PRIMER_INTERNAL_20_GC_PERCENT=52.174
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=0.00
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=0.00
PRIMER_LEFT_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_TH=37.44
PRIMER_INTERNAL_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n      5' AGCGTGU+2510\n          ||   C\n3' GGTTTTGCGTTTU+2518\n
PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_20_END_STABILITY=5.0700
PRIMER_RIGHT_20_END_STABILITY=3.2800
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_21_PENALTY=2.627395
PRIMER_RIGHT_21_PENALTY=2.627395
PRIMER_INTERNAL_21_PENALTY=3.606233
PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA
PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT
PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG
PRIMER_LEFT_21=52,21
PRIMER_RIGHT_21=72,21
PRIMER_INTERNAL_21=126,18
PRIMER_LEFT_21_TM=57.373
PRIMER_RIGHT_21_TM=57.373
PRIMER_INTERNAL_21_TM=58.394
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_INTERNAL_21_GC_PERCENT=66.667
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.14
PRIMER_INTERNAL_21_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n          3' GCCCU+2518\n
PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_21_END_STABILITY=4.5200
PRIMER_RIGHT_21_END_STABILITY=3.0600
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=3.9584
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_22_PENALTY=2.678056
PRIMER_RIGHT_22_PENALTY=2.678056
PRIMER_INTERNAL_22_PENALTY=3.829005
PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT
PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA
PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_22=58,21
PRIMER_RIGHT_22=78,21
PRIMER_INTERNAL_22=124,23
PRIMER_LEFT_22_TM=57.322
PRIMER_RIGHT_22_TM=57.322
PRIMER_INTERNAL_22_TM=60.829
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_INTERNAL_22_GC_PERCENT=56.522
PRIMER_INTERNAL_22_SELF_ANY_TH=7.82
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=0.00
PRIMER_RIGHT_22_HAIRPIN_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=36.42
PRIMER_INTERNAL_22_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n         3' CTAGCCCU+2518\n
PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_22_END_STABILITY=3.4100
PRIMER_RIGHT_22_END_STABILITY=2.1500
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=25.2513
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=19.0855
PRIMER_LEFT_23_PENALTY=2.780624
PRIMER_RIGHT_23_PENALTY=2.780624
PRIMER_INTERNAL_23_PENALTY=3.865064
PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT
PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_23=62,19
PRIMER_RIGHT_23=80,19
PRIMER_INTERNAL_23=120,21
PRIMER_LEFT_23_TM=57.219
PRIMER_RIGHT_23_TM=57.219
PRIMER_INTERNAL_23_TM=57.135
PRIMER_LEFT_23_GC_PERCENT=47.368
PRIMER_RIGHT_23_GC_PERCENT=47.368
PRIMER_INTERNAL_23_GC_PERCENT=52.381
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=0.00
PRIMER_RIGHT_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_23_END_STABILITY=3.5500
PRIMER_RIGHT_23_END_STABILITY=2.5200
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_24_PENALTY=2.999071
PRIMER_RIGHT_24_PENALTY=2.999071
PRIMER_INTERNAL_24_PENALTY=3.905453
PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA
PRIMER_LEFT_24=120,20
PRIMER_RIGHT_24=139,20
PRIMER_INTERNAL_24=127,18
PRIMER_LEFT_24_TM=61.999
PRIMER_RIGHT_24_TM=61.999
PRIMER_INTERNAL_24_TM=58.095
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_INTERNAL_24_GC_PERCENT=66.667
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=0.00
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=0.00
PRIMER_RIGHT_24_HAIRPIN_TH=37.44
PRIMER_INTERNAL_24_HAIRPIN_TH=36.42
PRIMER_RIGHT_24_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n       5' AGCGTGU+2510\n           ||   C\n3' AGGTTTTGCGTTTU+2518\n
PRIMER_INTERNAL_24_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n        3' AGCCCU+2518\n
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_24_END_STABILITY=5.0700
PRIMER_RIGHT_24_END_STABILITY=3.5300
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_25_PENALTY=2.999229
PRIMER_RIGHT_25_PENALTY=2.999229
PRIMER_INTERNAL_25_PENALTY=3.954555
PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG
PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC
PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_25=59,21
PRIMER_RIGHT_25=79,21
PRIMER_INTERNAL_25=120,23
PRIMER_LEFT_25_TM=57.001
PRIMER_RIGHT_25_TM=57.001
PRIMER_INTERNAL_25_TM=60.955
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_INTERNAL_25_GC_PERCENT=56.522
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=0.00
PRIMER_RIGHT_25_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=0.00
PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_25_END_STABILITY=3.6600
PRIMER_RIGHT_25_END_STABILITY=2.0800
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=30.7683
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=20.4326
PRIMER_LEFT_26_PENALTY=2.999229
PRIMER_RIGHT_26_PENALTY=2.999229
PRIMER_INTERNAL_26_PENALTY=4.240075
PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC
PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG
PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_26=57,21
PRIMER_RIGHT_26=77,21
PRIMER_INTERNAL_26=122,23
PRIMER_LEFT_26_TM=57.001
PRIMER_RIGHT_26_TM=57.001
PRIMER_INTERNAL_26_TM=61.240
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=56.522
PRIMER_INTERNAL_26_SELF_ANY_TH=7.82
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=0.00
PRIMER_INTERNAL_26_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' CAAAACGCAAAGCACGCTCC-CGA 3'\n        ||   |||  |||   ||\n    3' AGCCC-TCGCACGAAACGCAAAAC 5'\n
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=0.00
PRIMER_LEFT_26_HAIRPIN_TH=0.00
PRIMER_RIGHT_26_HAIRPIN_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=36.42
PRIMER_INTERNAL_26_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CAAAACGCAAAGCACGCTU+2510\n                 ||  U+2502\n             3' AGCCCU+2518\n
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=3.5100
PRIMER_RIGHT_26_END_STABILITY=2.9000
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=20.2809
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.6247
PRIMER_LEFT_27_PENALTY=3.455227
PRIMER_RIGHT_27_PENALTY=3.455227
PRIMER_INTERNAL_27_PENALTY=4.486709
PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_27=118,21
PRIMER_RIGHT_27=138,21
PRIMER_INTERNAL_27=119,22
PRIMER_LEFT_27_TM=61.455
PRIMER_RIGHT_27_TM=61.455
PRIMER_INTERNAL_27_TM=57.513
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=50.000
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=0.00
PRIMER_RIGHT_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=0.00
PRIMER_RIGHT_27_SELF_END_TH=0.00
PRIMER_LEFT_27_HAIRPIN_TH=0.00
PRIMER_RIGHT_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=5.3400
PRIMER_RIGHT_27_END_STABILITY=3.0100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_28_PENALTY=3.677573
PRIMER_RIGHT_28_PENALTY=3.677573
PRIMER_INTERNAL_28_PENALTY=4.513970
PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC
PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_28=123,20
PRIMER_RIGHT_28=142,20
PRIMER_INTERNAL_28=118,24
PRIMER_LEFT_28_TM=62.678
PRIMER_RIGHT_28_TM=62.678
PRIMER_INTERNAL_28_TM=59.486
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_INTERNAL_28_GC_PERCENT=50.000
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=37.44
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n 5' GGGAGCGTGU+2510\n        ||   C\n3' TTTTGCGTTTU+2518\n
PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_28_END_STABILITY=4.3000
PRIMER_RIGHT_28_END_STABILITY=2.4300
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.1346
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_29_PENALTY=4.100862
PRIMER_RIGHT_29_PENALTY=4.100862
PRIMER_INTERNAL_29_PENALTY=5.029817
PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC
PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC
PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_29=128,19
PRIMER_RIGHT_29=146,19
PRIMER_INTERNAL_29=123,24
PRIMER_LEFT_29_TM=62.101
PRIMER_RIGHT_29_TM=62.101
PRIMER_INTERNAL_29_TM=61.030
PRIMER_LEFT_29_GC_PERCENT=63.158
PRIMER_RIGHT_29_GC_PERCENT=63.158
PRIMER_INTERNAL_29_GC_PERCENT=54.167
PRIMER_INTERNAL_29_SELF_ANY_TH=7.82
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGATC 3'\n       ||   |||  |||   ||\n 3' CTAGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=38.29
PRIMER_RIGHT_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=36.42
PRIMER_LEFT_29_HAIRPIN_STUCT=Tm: 38.3&deg;C  dG: -93 cal/mol  dH: -22500 cal/mol  dS: -72 cal/mol*K\n5' GCAAAGCACGCTU+2510\n           ||  U+2502\n     3' CTAGCCCU+2518\n
PRIMER_INTERNAL_29_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n          3' CTAGCCCU+2518\n
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_29_END_STABILITY=3.6900
PRIMER_RIGHT_29_END_STABILITY=3.6800
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0362
=
PRIMER_TASK=pick_primer_list
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_primer_list_and_file_flag_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52
PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 17, high repeat similarity 3, ok 34
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=30
PRIMER_RIGHT_NUM_RETURNED=30
PRIMER_INTERNAL_NUM_RETURNED=30
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.527529
PRIMER_RIGHT_0_PENALTY=0.527529
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=80,20
PRIMER_INTERNAL_0=66,20
PRIMER_LEFT_0_TM=59.528
PRIMER_RIGHT_0_TM=59.528
PRIMER_INTERNAL_0_TM=59.273
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_LEFT_0_SELF_ANY_TH=10.70
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n     5' TTAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGATT 5'\n
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n              5' GGGCCATTCGATCCCGACCC 3'\n                  ||||\n3' CCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=40.47
PRIMER_RIGHT_0_HAIRPIN_TH=41.64
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_LEFT_0_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' TTAGTGGGCCATU+2510\n        |||    T\n     3' CCCTAGCU+2518\n
PRIMER_RIGHT_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n     5' GGGATCGU+2510\n        |||    A\n3' AATCACCCGGTAU+2518\n
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n    3' CCCAGCCCU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_RIGHT_0_END_STABILITY=2.2400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266
PRIMER_LEFT_1_PENALTY=0.606923
PRIMER_RIGHT_1_PENALTY=0.606923
PRIMER_INTERNAL_1_PENALTY=1.416589
PRIMER_LEFT_1_SEQUENCE=CCCGACCCAAATCGATAGCT
PRIMER_RIGHT_1_SEQUENCE=AGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC
PRIMER_LEFT_1=78,20
PRIMER_RIGHT_1=97,20
PRIMER_INTERNAL_1=64,21
PRIMER_LEFT_1_TM=59.607
PRIMER_RIGHT_1_TM=59.607
PRIMER_INTERNAL_1_TM=59.583
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_SELF_ANY_TH=7.03
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=1.71
PRIMER_RIGHT_1_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n      5' AGCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCGA 5'\n
PRIMER_INTERNAL_1_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n            5' GTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.13
PRIMER_RIGHT_1_HAIRPIN_TH=41.74
PRIMER_INTERNAL_1_HAIRPIN_TH=41.60
PRIMER_LEFT_1_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n    5' CCCGACCCU+2510\n         |||   U+2502\n3' TCGATAGCTAAAU+2518\n
PRIMER_RIGHT_1_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' AGCTATCGATTTU+2510\n         |||   U+2502\n    3' GGGCTGGGU+2518\n
PRIMER_INTERNAL_1_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n       3' CCAGCCCU+2518\n
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_1_END_STABILITY=3.3200
PRIMER_RIGHT_1_END_STABILITY=5.1400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_2_PENALTY=0.608403
PRIMER_RIGHT_2_PENALTY=0.608403
PRIMER_INTERNAL_2_PENALTY=1.438741
PRIMER_LEFT_2_SEQUENCE=TCCCGACCCAAATCGATAGC
PRIMER_RIGHT_2_SEQUENCE=GCTATCGATTTGGGTCGGGA
PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA
PRIMER_LEFT_2=77,20
PRIMER_RIGHT_2=96,20
PRIMER_INTERNAL_2=67,20
PRIMER_LEFT_2_TM=59.608
PRIMER_RIGHT_2_TM=59.608
PRIMER_INTERNAL_2_TM=58.561
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=1.71
PRIMER_RIGHT_2_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n        5' GCTATCGATTTGGGTCGGGA 3'\n              ||||||\n3' AGGGCTGGGTTTAGCTATCG 5'\n
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=45.13
PRIMER_RIGHT_2_HAIRPIN_TH=41.74
PRIMER_INTERNAL_2_HAIRPIN_TH=41.60
PRIMER_LEFT_2_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n  5' TCCCGACCCU+2510\n        |||   U+2502\n3' CGATAGCTAAAU+2518\n
PRIMER_RIGHT_2_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GCTATCGATTTU+2510\n        |||   U+2502\n  3' AGGGCTGGGU+2518\n
PRIMER_INTERNAL_2_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n  3' ACCCAGCCCU+2518\n
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_2_END_STABILITY=2.9700
PRIMER_RIGHT_2_END_STABILITY=5.1400
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=2.4428
PRIMER_LEFT_3_PENALTY=0.713597
PRIMER_RIGHT_3_PENALTY=0.713597
PRIMER_INTERNAL_3_PENALTY=1.438741
PRIMER_LEFT_3_SEQUENCE=GACATGACACTGACGATGCA
PRIMER_RIGHT_3_SEQUENCE=TGCATCGTCAGTGTCATGTC
PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC
PRIMER_LEFT_3=11,20
PRIMER_RIGHT_3=30,20
PRIMER_INTERNAL_3=65,20
PRIMER_LEFT_3_TM=58.286
PRIMER_RIGHT_3_TM=58.286
PRIMER_INTERNAL_3_TM=58.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=41.60
PRIMER_INTERNAL_3_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TGGGCCATTCGATU+2510\n           |||  U+2502\n      3' CCAGCCCU+2518\n
PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_3_END_STABILITY=3.9600
PRIMER_RIGHT_3_END_STABILITY=3.0600
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=16.7219
PRIMER_LEFT_4_PENALTY=0.966496
PRIMER_RIGHT_4_PENALTY=0.966496
PRIMER_INTERNAL_4_PENALTY=1.650306
PRIMER_LEFT_4_SEQUENCE=CGATCCCGACCCAAATCGAT
PRIMER_RIGHT_4_SEQUENCE=ATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA
PRIMER_LEFT_4=74,20
PRIMER_RIGHT_4=93,20
PRIMER_INTERNAL_4=66,21
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.966
PRIMER_INTERNAL_4_TM=60.650
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_INTERNAL_4_SELF_ANY_TH=2.18
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n               5' GGGCCATTCGATCCCGACCCA 3'\n                   ||||\n3' ACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=45.13
PRIMER_RIGHT_4_HAIRPIN_TH=41.74
PRIMER_INTERNAL_4_HAIRPIN_TH=41.60
PRIMER_LEFT_4_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' CGATCCCGACCCU+2510\n         |||   U+2502\n    3' TAGCTAAAU+2518\n
PRIMER_RIGHT_4_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n    5' ATCGATTTU+2510\n         |||   U+2502\n3' GCTAGGGCTGGGU+2518\n
PRIMER_INTERNAL_4_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n   3' ACCCAGCCCU+2518\n
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_4_END_STABILITY=3.5900
PRIMER_RIGHT_4_END_STABILITY=3.6900
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_5_PENALTY=0.967319
PRIMER_RIGHT_5_PENALTY=1.001812
PRIMER_INTERNAL_5_PENALTY=1.650306
PRIMER_LEFT_5_SEQUENCE=CATTCGATCCCGACCCAAAT
PRIMER_RIGHT_5_SEQUENCE=GTCTGCATCGTCAGTGTCAT
PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC
PRIMER_LEFT_5=70,20
PRIMER_RIGHT_5=33,20
PRIMER_INTERNAL_5=65,21
PRIMER_LEFT_5_TM=58.033
PRIMER_RIGHT_5_TM=57.998
PRIMER_INTERNAL_5_TM=60.650
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_INTERNAL_5_GC_PERCENT=66.667
PRIMER_INTERNAL_5_SELF_ANY_TH=0.00
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=45.50
PRIMER_RIGHT_5_HAIRPIN_TH=35.63
PRIMER_INTERNAL_5_HAIRPIN_TH=41.60
PRIMER_LEFT_5_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n    5' CATTCGATU+2510\n          |||  U+2502\n3' TAAACCCAGCCCU+2518\n
PRIMER_RIGHT_5_HAIRPIN_STUCT=Tm: 35.6&deg;C  dG: 127 cal/mol  dH: -28600 cal/mol  dS: -93 cal/mol*K\n    5' GTCTGCATU+2510\n         |||   U+2502\n3' TACTGTGACTGCU+2518\n
PRIMER_INTERNAL_5_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TGGGCCATTCGATU+2510\n           |||  U+2502\n     3' CCCAGCCCU+2518\n
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_5_END_STABILITY=2.3200
PRIMER_RIGHT_5_END_STABILITY=3.0600
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=8.6039
PRIMER_LEFT_6_PENALTY=1.000429
PRIMER_RIGHT_6_PENALTY=1.001812
PRIMER_INTERNAL_6_PENALTY=2.095978
PRIMER_LEFT_6_SEQUENCE=CATGACACTGACGATGCAGA
PRIMER_RIGHT_6_SEQUENCE=GCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA
PRIMER_LEFT_6=13,20
PRIMER_RIGHT_6=29,20
PRIMER_INTERNAL_6=67,21
PRIMER_LEFT_6_TM=58.000
PRIMER_RIGHT_6_TM=57.998
PRIMER_INTERNAL_6_TM=58.904
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=0.00
PRIMER_LEFT_6_SELF_ANY_TH=10.65
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_LEFT_6_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -3380 cal/mol  dH: -84400 cal/mol  dS: -261 cal/mol*K\n5' CATGACACTGA--CGATGCAGA 3'\n          |||   ||   |||\n      3' AGACGTAGCA--GTCACAGTAC 5'\n
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=39.20
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=41.60
PRIMER_LEFT_6_HAIRPIN_STUCT=Tm: 39.2&deg;C  dG: -189 cal/mol  dH: -26900 cal/mol  dS: -86 cal/mol*K\n5' CATGACACTGACGU+2510\n          |||   U+2502\n      3' AGACGTAU+2518\n
PRIMER_INTERNAL_6_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n 3' AACCCAGCCCU+2518\n
PRIMER_LEFT_6_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_6_END_STABILITY=4.2600
PRIMER_RIGHT_6_END_STABILITY=3.4100
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=1.2632
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_7_PENALTY=1.001812
PRIMER_RIGHT_7_PENALTY=1.142220
PRIMER_INTERNAL_7_PENALTY=2.360266
PRIMER_LEFT_7_SEQUENCE=ATGACACTGACGATGCAGAC
PRIMER_RIGHT_7_SEQUENCE=GGGATCGAATGGCCCACTA
PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_7=14,20
PRIMER_RIGHT_7=80,19
PRIMER_INTERNAL_7=63,21
PRIMER_LEFT_7_TM=57.998
PRIMER_RIGHT_7_TM=58.858
PRIMER_INTERNAL_7_TM=58.640
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=57.895
PRIMER_INTERNAL_7_GC_PERCENT=61.905
PRIMER_INTERNAL_7_SELF_ANY_TH=7.03
PRIMER_LEFT_7_SELF_ANY_TH=10.65
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_LEFT_7_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -3380 cal/mol  dH: -84400 cal/mol  dS: -261 cal/mol*K\n5' ATGACACTGA--CGATGCAGAC 3'\n         |||   ||   |||\n    3' CAGACGTAGCA--GTCACAGTA 5'\n
PRIMER_INTERNAL_7_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n          5' AGTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=39.20
PRIMER_RIGHT_7_HAIRPIN_TH=41.64
PRIMER_INTERNAL_7_HAIRPIN_TH=41.60
PRIMER_LEFT_7_HAIRPIN_STUCT=Tm: 39.2&deg;C  dG: -189 cal/mol  dH: -26900 cal/mol  dS: -86 cal/mol*K\n5' ATGACACTGACGU+2510\n         |||   U+2502\n    3' CAGACGTAU+2518\n
PRIMER_RIGHT_7_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n    5' GGGATCGU+2510\n       |||    A\n3' ATCACCCGGTAU+2518\n
PRIMER_INTERNAL_7_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' AGTGGGCCATTCGATU+2510\n             |||  U+2502\n         3' CAGCCCU+2518\n
PRIMER_LEFT_7_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_7_END_STABILITY=3.5100
PRIMER_RIGHT_7_END_STABILITY=2.7400
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=16.1639
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=7.8928
PRIMER_LEFT_8_PENALTY=1.001812
PRIMER_RIGHT_8_PENALTY=1.316792
PRIMER_INTERNAL_8_PENALTY=2.456420
PRIMER_LEFT_8_SEQUENCE=AGACATGACACTGACGATGC
PRIMER_RIGHT_8_SEQUENCE=CGTAGCTATCGATTTGGGTCG
PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA
PRIMER_LEFT_8=10,20
PRIMER_RIGHT_8=100,21
PRIMER_INTERNAL_8=63,20
PRIMER_LEFT_8_TM=57.998
PRIMER_RIGHT_8_TM=58.683
PRIMER_INTERNAL_8_TM=57.544
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_INTERNAL_8_SELF_ANY_TH=7.03
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=1.71
PRIMER_RIGHT_8_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n 5' CGTAGCTATCGATTTGGGTCG 3'\n           ||||||\n3' GCTGGGTTTAGCTATCGATGC 5'\n
PRIMER_INTERNAL_8_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n         5' AGTGGGCCATTCGATCCCGA 3'\n              ||| |||\n3' AGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=42.74
PRIMER_INTERNAL_8_HAIRPIN_TH=39.49
PRIMER_RIGHT_8_HAIRPIN_STUCT=Tm: 42.7&deg;C  dG: -478 cal/mol  dH: -26300 cal/mol  dS: -83 cal/mol*K\n5' CGTAGCTATCGATTTU+2510\n            |||   U+2502\n         3' GCTGGGU+2518\n
PRIMER_INTERNAL_8_HAIRPIN_STUCT=Tm: 39.5&deg;C  dG: -197 cal/mol  dH: -24700 cal/mol  dS: -79 cal/mol*K\n5' AGTGGGCCATU+2510\n      |||    T\n 3' AGCCCTAGCU+2518\n
PRIMER_LEFT_8_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_8_END_STABILITY=3.9100
PRIMER_RIGHT_8_END_STABILITY=4.7900
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=7.5573
PRIMER_LEFT_9_PENALTY=1.107410
PRIMER_RIGHT_9_PENALTY=1.383135
PRIMER_INTERNAL_9_PENALTY=2.511372
PRIMER_LEFT_9_SEQUENCE=CCATTCGATCCCGACCCAAA
PRIMER_RIGHT_9_SEQUENCE=TAGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC
PRIMER_LEFT_9=69,20
PRIMER_RIGHT_9=98,21
PRIMER_INTERNAL_9=64,20
PRIMER_LEFT_9_TM=60.107
PRIMER_RIGHT_9_TM=59.383
PRIMER_INTERNAL_9_TM=57.489
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_INTERNAL_9_GC_PERCENT=65.000
PRIMER_INTERNAL_9_SELF_ANY_TH=7.03
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=1.71
PRIMER_RIGHT_9_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n     5' TAGCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCGAT 5'\n
PRIMER_INTERNAL_9_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n           5' GTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=45.50
PRIMER_RIGHT_9_HAIRPIN_TH=41.74
PRIMER_INTERNAL_9_HAIRPIN_TH=41.60
PRIMER_LEFT_9_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n  5' CCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_RIGHT_9_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' TAGCTATCGATTTU+2510\n          |||   U+2502\n     3' GGGCTGGGU+2518\n
PRIMER_INTERNAL_9_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n        3' CAGCCCU+2518\n
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_9_END_STABILITY=3.2800
PRIMER_RIGHT_9_END_STABILITY=5.1400
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_10_PENALTY=1.126409
PRIMER_RIGHT_10_PENALTY=1.559940
PRIMER_INTERNAL_10_PENALTY=2.626619
PRIMER_LEFT_10_SEQUENCE=CATTCGATCCCGACCCAAATC
PRIMER_RIGHT_10_SEQUENCE=TCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_10=70,21
PRIMER_RIGHT_10=92,19
PRIMER_INTERNAL_10=63,22
PRIMER_LEFT_10_TM=59.126
PRIMER_RIGHT_10_TM=59.560
PRIMER_INTERNAL_10_TM=60.627
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=57.895
PRIMER_INTERNAL_10_GC_PERCENT=63.636
PRIMER_INTERNAL_10_SELF_ANY_TH=7.03
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n           5' AGTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=45.50
PRIMER_RIGHT_10_HAIRPIN_TH=41.74
PRIMER_INTERNAL_10_HAIRPIN_TH=41.60
PRIMER_LEFT_10_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n     5' CATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_RIGHT_10_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n     5' TCGATTTU+2510\n         |||   U+2502\n3' GCTAGGGCTGGGU+2518\n
PRIMER_INTERNAL_10_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' AGTGGGCCATTCGATU+2510\n             |||  U+2502\n        3' CCAGCCCU+2518\n
PRIMER_LEFT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_10_END_STABILITY=2.1700
PRIMER_RIGHT_10_END_STABILITY=3.6900
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_11_PENALTY=1.142220
PRIMER_RIGHT_11_PENALTY=1.559940
PRIMER_INTERNAL_11_PENALTY=2.783921
PRIMER_LEFT_11_SEQUENCE=TAGTGGGCCATTCGATCCC
PRIMER_RIGHT_11_SEQUENCE=CGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_11=62,19
PRIMER_RIGHT_11=91,19
PRIMER_INTERNAL_11=67,22
PRIMER_LEFT_11_TM=58.858
PRIMER_RIGHT_11_TM=59.560
PRIMER_INTERNAL_11_TM=59.216
PRIMER_LEFT_11_GC_PERCENT=57.895
PRIMER_RIGHT_11_GC_PERCENT=57.895
PRIMER_INTERNAL_11_GC_PERCENT=59.091
PRIMER_INTERNAL_11_SELF_ANY_TH=0.00
PRIMER_LEFT_11_SELF_ANY_TH=10.70
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_LEFT_11_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n      5' TAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=40.47
PRIMER_RIGHT_11_HAIRPIN_TH=42.82
PRIMER_INTERNAL_11_HAIRPIN_TH=41.60
PRIMER_LEFT_11_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' TAGTGGGCCATU+2510\n       |||    T\n    3' CCCTAGCU+2518\n
PRIMER_RIGHT_11_HAIRPIN_STUCT=Tm: 42.8&deg;C  dG: -584 cal/mol  dH: -31700 cal/mol  dS: -100 cal/mol*K\n 5' CGATTTGGGU+2510\n    ||||     U+2502\n3' AGCTAGGGCTU+2518\n
PRIMER_INTERNAL_11_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_LEFT_11_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_11_END_STABILITY=3.8500
PRIMER_RIGHT_11_END_STABILITY=3.5900
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_12_PENALTY=1.179094
PRIMER_RIGHT_12_PENALTY=1.568623
PRIMER_INTERNAL_12_PENALTY=2.885177
PRIMER_LEFT_12_SEQUENCE=TTCGATCCCGACCCAAATCG
PRIMER_RIGHT_12_SEQUENCE=GGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA
PRIMER_LEFT_12=72,20
PRIMER_RIGHT_12=79,20
PRIMER_INTERNAL_12=66,22
PRIMER_LEFT_12_TM=60.179
PRIMER_RIGHT_12_TM=57.431
PRIMER_INTERNAL_12_TM=60.885
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=63.636
PRIMER_INTERNAL_12_SELF_ANY_TH=2.18
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n                5' GGGCCATTCGATCCCGACCCAA 3'\n                    ||||\n3' AACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=45.50
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.60
PRIMER_LEFT_12_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n        5' TTCGATU+2510\n            |||  U+2502\n3' GCTAAACCCAGCCCU+2518\n
PRIMER_INTERNAL_12_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n  3' AACCCAGCCCU+2518\n
PRIMER_LEFT_12_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_12_END_STABILITY=3.3400
PRIMER_RIGHT_12_END_STABILITY=1.7300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=2.4855
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=4.2003
PRIMER_LEFT_13_PENALTY=1.256117
PRIMER_RIGHT_13_PENALTY=1.673833
PRIMER_INTERNAL_13_PENALTY=3.114431
PRIMER_LEFT_13_SEQUENCE=ATTCGATCCCGACCCAAATC
PRIMER_RIGHT_13_SEQUENCE=GTAGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_13=71,20
PRIMER_RIGHT_13=99,21
PRIMER_INTERNAL_13=62,23
PRIMER_LEFT_13_TM=57.744
PRIMER_RIGHT_13_TM=58.326
PRIMER_INTERNAL_13_TM=60.114
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_INTERNAL_13_GC_PERCENT=60.870
PRIMER_INTERNAL_13_SELF_ANY_TH=7.03
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=1.71
PRIMER_RIGHT_13_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n   5' GTAGCTATCGATTTGGGTCGG 3'\n            ||||||\n3' GGCTGGGTTTAGCTATCGATG 5'\n
PRIMER_INTERNAL_13_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n          5' TAGTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=45.50
PRIMER_RIGHT_13_HAIRPIN_TH=41.74
PRIMER_INTERNAL_13_HAIRPIN_TH=41.60
PRIMER_LEFT_13_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n      5' ATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_RIGHT_13_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GTAGCTATCGATTTU+2510\n           |||   U+2502\n       3' GGCTGGGU+2518\n
PRIMER_INTERNAL_13_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TAGTGGGCCATTCGATU+2510\n              |||  U+2502\n         3' CCAGCCCU+2518\n
PRIMER_LEFT_13_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_13_END_STABILITY=2.1700
PRIMER_RIGHT_13_END_STABILITY=4.7900
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=2.2809
PRIMER_LEFT_14_PENALTY=1.316792
PRIMER_RIGHT_14_PENALTY=1.726673
PRIMER_INTERNAL_14_PENALTY=3.510595
PRIMER_LEFT_14_SEQUENCE=CGACCCAAATCGATAGCTACG
PRIMER_RIGHT_14_SEQUENCE=TGCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC
PRIMER_LEFT_14=80,21
PRIMER_RIGHT_14=30,21
PRIMER_INTERNAL_14=64,22
PRIMER_LEFT_14_TM=58.683
PRIMER_RIGHT_14_TM=59.727
PRIMER_INTERNAL_14_TM=61.511
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_INTERNAL_14_GC_PERCENT=68.182
PRIMER_INTERNAL_14_SELF_ANY_TH=7.03
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n             5' GTGGGCCATTCGATCCCGACCC 3'\n                 ||| |||\n3' CCCAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=2.30
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_STUCT=Tm: 2.3&deg;C  dG: -3380 cal/mol  dH: -62200 cal/mol  dS: -190 cal/mol*K\n5' CGACCCAAATCGATAGCTA-CG 3'\n                |||||| ||\n           3' GCATCGATAGCTAAACCCAGC 5'\n
PRIMER_LEFT_14_HAIRPIN_TH=45.13
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=41.60
PRIMER_LEFT_14_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n         5' CGACCCU+2510\n            |||   U+2502\n3' GCATCGATAGCTAAAU+2518\n
PRIMER_INTERNAL_14_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n      3' CCCAGCCCU+2518\n
PRIMER_LEFT_14_LIBRARY_MISPRIMING=10.00, MER4A a consensus
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_14_END_STABILITY=3.5100
PRIMER_RIGHT_14_END_STABILITY=3.4100
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5113
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_15_PENALTY=1.383135
PRIMER_RIGHT_15_PENALTY=1.728621
PRIMER_INTERNAL_15_PENALTY=3.685489
PRIMER_LEFT_15_SEQUENCE=CCCGACCCAAATCGATAGCTA
PRIMER_RIGHT_15_SEQUENCE=TATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_15=78,21
PRIMER_RIGHT_15=94,21
PRIMER_INTERNAL_15=67,23
PRIMER_LEFT_15_TM=59.383
PRIMER_RIGHT_15_TM=59.729
PRIMER_INTERNAL_15_TM=59.315
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_INTERNAL_15_GC_PERCENT=56.522
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=1.71
PRIMER_RIGHT_15_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n             5' TATCGATTTGGGTCGGGATCG 3'\n                 ||||||\n3' GCTAGGGCTGGGTTTAGCTAT 5'\n
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=45.13
PRIMER_RIGHT_15_HAIRPIN_TH=41.74
PRIMER_INTERNAL_15_HAIRPIN_TH=41.60
PRIMER_LEFT_15_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n     5' CCCGACCCU+2510\n          |||   U+2502\n3' ATCGATAGCTAAAU+2518\n
PRIMER_RIGHT_15_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n   5' TATCGATTTU+2510\n         |||   U+2502\n3' GCTAGGGCTGGGU+2518\n
PRIMER_INTERNAL_15_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' GGCCATTCGATU+2510\n          |||  U+2502\n3' TAAACCCAGCCCU+2518\n
PRIMER_LEFT_15_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_15_END_STABILITY=3.3200
PRIMER_RIGHT_15_END_STABILITY=3.6900
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=13.2273
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_16_PENALTY=1.475039
PRIMER_RIGHT_16_PENALTY=1.914379
PRIMER_INTERNAL_16_PENALTY=3.807618
PRIMER_LEFT_16_SEQUENCE=CCATTCGATCCCGACCCAA
PRIMER_RIGHT_16_SEQUENCE=GCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_16=69,19
PRIMER_RIGHT_16=96,19
PRIMER_INTERNAL_16=62,22
PRIMER_LEFT_16_TM=59.475
PRIMER_RIGHT_16_TM=58.086
PRIMER_INTERNAL_16_TM=58.192
PRIMER_LEFT_16_GC_PERCENT=57.895
PRIMER_RIGHT_16_GC_PERCENT=57.895
PRIMER_INTERNAL_16_GC_PERCENT=59.091
PRIMER_INTERNAL_16_SELF_ANY_TH=7.03
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=1.71
PRIMER_RIGHT_16_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n       5' GCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCG 5'\n
PRIMER_INTERNAL_16_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n         5' TAGTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=45.50
PRIMER_RIGHT_16_HAIRPIN_TH=41.74
PRIMER_INTERNAL_16_HAIRPIN_TH=41.60
PRIMER_LEFT_16_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' CCATTCGATU+2510\n        |||  U+2502\n3' AACCCAGCCCU+2518\n
PRIMER_RIGHT_16_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GCTATCGATTTU+2510\n        |||   U+2502\n   3' GGGCTGGGU+2518\n
PRIMER_INTERNAL_16_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TAGTGGGCCATTCGATU+2510\n              |||  U+2502\n          3' CAGCCCU+2518\n
PRIMER_LEFT_16_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_16_END_STABILITY=4.1200
PRIMER_RIGHT_16_END_STABILITY=5.1400
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_17_PENALTY=1.559940
PRIMER_RIGHT_17_PENALTY=1.926077
PRIMER_INTERNAL_17_PENALTY=3.826170
PRIMER_LEFT_17_SEQUENCE=CGATCCCGACCCAAATCGA
PRIMER_RIGHT_17_SEQUENCE=GGGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA
PRIMER_LEFT_17=74,19
PRIMER_RIGHT_17=80,21
PRIMER_INTERNAL_17=68,21
PRIMER_LEFT_17_TM=59.560
PRIMER_RIGHT_17_TM=59.926
PRIMER_INTERNAL_17_TM=57.174
PRIMER_LEFT_17_GC_PERCENT=57.895
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=45.13
PRIMER_RIGHT_17_HAIRPIN_TH=41.64
PRIMER_INTERNAL_17_HAIRPIN_TH=41.60
PRIMER_LEFT_17_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' CGATCCCGACCCU+2510\n         |||   U+2502\n     3' AGCTAAAU+2518\n
PRIMER_RIGHT_17_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n      5' GGGATCGU+2510\n         |||    A\n3' TAATCACCCGGTAU+2518\n
PRIMER_INTERNAL_17_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' GCCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_LEFT_17_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_17_END_STABILITY=3.5900
PRIMER_RIGHT_17_END_STABILITY=1.7300
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=12.3668
PRIMER_LEFT_18_PENALTY=1.559940
PRIMER_RIGHT_18_PENALTY=1.960819
PRIMER_INTERNAL_18_PENALTY=3.864865
PRIMER_LEFT_18_SEQUENCE=TCGATCCCGACCCAAATCG
PRIMER_RIGHT_18_SEQUENCE=CTATCGATTTGGGTCGGGATC
PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT
PRIMER_LEFT_18=73,19
PRIMER_RIGHT_18=95,21
PRIMER_INTERNAL_18=37,23
PRIMER_LEFT_18_TM=59.560
PRIMER_RIGHT_18_TM=58.039
PRIMER_INTERNAL_18_TM=59.135
PRIMER_LEFT_18_GC_PERCENT=57.895
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_INTERNAL_18_GC_PERCENT=52.174
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=1.71
PRIMER_RIGHT_18_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n           5' CTATCGATTTGGGTCGGGATC 3'\n                ||||||\n3' CTAGGGCTGGGTTTAGCTATC 5'\n
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=40.30
PRIMER_RIGHT_18_HAIRPIN_TH=41.74
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_HAIRPIN_STUCT=Tm: 40.3&deg;C  dG: -227 cal/mol  dH: -21600 cal/mol  dS: -69 cal/mol*K\n         5' TCGATU+2510\n            |||  U+2502\n3' GCTAAACCCAGCCCU+2518\n
PRIMER_RIGHT_18_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n 5' CTATCGATTTU+2510\n        |||   U+2502\n3' CTAGGGCTGGGU+2518\n
PRIMER_LEFT_18_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_18_END_STABILITY=3.3400
PRIMER_RIGHT_18_END_STABILITY=3.3600
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=0.0363
PRIMER_LEFT_19_PENALTY=1.568623
PRIMER_RIGHT_19_PENALTY=1.997913
PRIMER_INTERNAL_19_PENALTY=3.881423
PRIMER_LEFT_19_SEQUENCE=ATTAGTGGGCCATTCGATCC
PRIMER_RIGHT_19_SEQUENCE=GCTATCGATTTGGGTCGGGAT
PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_19=60,20
PRIMER_RIGHT_19=96,21
PRIMER_INTERNAL_19=62,21
PRIMER_LEFT_19_TM=57.431
PRIMER_RIGHT_19_TM=59.998
PRIMER_INTERNAL_19_TM=57.119
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_INTERNAL_19_GC_PERCENT=57.143
PRIMER_INTERNAL_19_SELF_ANY_TH=7.03
PRIMER_LEFT_19_SELF_ANY_TH=10.70
PRIMER_RIGHT_19_SELF_ANY_TH=1.71
PRIMER_LEFT_19_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n   5' ATTAGTGGGCCATTCGATCC 3'\n           ||| |||\n3' CCTAGCTTACCGGGTGATTA 5'\n
PRIMER_RIGHT_19_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n         5' GCTATCGATTTGGGTCGGGAT 3'\n               ||||||\n3' TAGGGCTGGGTTTAGCTATCG 5'\n
PRIMER_INTERNAL_19_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n        5' TAGTGGGCCATTCGATCCCGA 3'\n              ||| |||\n3' AGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=41.74
PRIMER_INTERNAL_19_HAIRPIN_TH=39.49
PRIMER_RIGHT_19_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GCTATCGATTTU+2510\n        |||   U+2502\n 3' TAGGGCTGGGU+2518\n
PRIMER_INTERNAL_19_HAIRPIN_STUCT=Tm: 39.5&deg;C  dG: -197 cal/mol  dH: -24700 cal/mol  dS: -79 cal/mol*K\n5' TAGTGGGCCATU+2510\n       |||    T\n  3' AGCCCTAGCU+2518\n
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_19_END_STABILITY=3.3600
PRIMER_RIGHT_19_END_STABILITY=3.8500
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=9.4710
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=1.673833
PRIMER_RIGHT_20_PENALTY=2.038681
PRIMER_INTERNAL_20_PENALTY=3.936624
PRIMER_LEFT_20_SEQUENCE=CCGACCCAAATCGATAGCTAC
PRIMER_RIGHT_20_SEQUENCE=TCGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA
PRIMER_LEFT_20=79,21
PRIMER_RIGHT_20=92,20
PRIMER_INTERNAL_20=65,22
PRIMER_LEFT_20_TM=58.326
PRIMER_RIGHT_20_TM=61.039
PRIMER_INTERNAL_20_TM=61.937
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_INTERNAL_20_GC_PERCENT=63.636
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=3.47
PRIMER_RIGHT_20_SELF_ANY_STUCT=Tm: 3.5&deg;C  dG: -2609 cal/mol  dH: -71000 cal/mol  dS: -221 cal/mol*K\n5' TCGATTTGGGTCGGGATCGA 3'\n   |||||          |||||\n3' AGCTAGGGCTGGGTTTAGCT 5'\n
PRIMER_INTERNAL_20_SELF_END_TH=3.99
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=3.47
PRIMER_RIGHT_20_SELF_END_STUCT=Tm: 3.5&deg;C  dG: -2609 cal/mol  dH: -71000 cal/mol  dS: -221 cal/mol*K\n5' TCGATTTGGGTCGGGATCGA 3'\n   |||||          |||||\n3' AGCTAGGGCTGGGTTTAGCT 5'\n
PRIMER_INTERNAL_20_SELF_END_STUCT=Tm: 4.0&deg;C  dG: -782 cal/mol  dH: -87600 cal/mol  dS: -280 cal/mol*K\n5' TGGGCCATTCGATCCCGA----CCCA 3'\n   ||||    |||    |||    ||||\n3' ACCC----AGCCCTAGCTTACCGGGT 5'\n
PRIMER_LEFT_20_HAIRPIN_TH=45.13
PRIMER_RIGHT_20_HAIRPIN_TH=45.48
PRIMER_INTERNAL_20_HAIRPIN_TH=42.67
PRIMER_LEFT_20_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n       5' CCGACCCU+2510\n           |||   U+2502\n3' CATCGATAGCTAAAU+2518\n
PRIMER_RIGHT_20_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -948 cal/mol  dH: -35600 cal/mol  dS: -112 cal/mol*K\n5' TCGATTTGGGU+2510\n   |||||     U+2502\n3' AGCTAGGGCTU+2518\n
PRIMER_INTERNAL_20_HAIRPIN_STUCT=Tm: 42.7&deg;C  dG: -788 cal/mol  dH: -43900 cal/mol  dS: -139 cal/mol*K\n5' TGGGCCATTCGATU+2510\n   ||||    |||  U+2502\n    3' ACCCAGCCCU+2518\n
PRIMER_LEFT_20_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_20_END_STABILITY=3.5800
PRIMER_RIGHT_20_END_STABILITY=3.5900
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.3824
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_21_PENALTY=1.726673
PRIMER_RIGHT_21_PENALTY=2.150848
PRIMER_INTERNAL_21_PENALTY=3.956741
PRIMER_LEFT_21_SEQUENCE=AGACATGACACTGACGATGCA
PRIMER_RIGHT_21_SEQUENCE=GCATCGTCAGTGTCATGTCTA
PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_LEFT_21=10,21
PRIMER_RIGHT_21=29,21
PRIMER_INTERNAL_21=67,19
PRIMER_LEFT_21_TM=59.727
PRIMER_RIGHT_21_TM=57.849
PRIMER_INTERNAL_21_TM=57.043
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_INTERNAL_21_GC_PERCENT=68.421
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.60
PRIMER_INTERNAL_21_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n   3' CCCAGCCCU+2518\n
PRIMER_LEFT_21_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_21_END_STABILITY=3.9600
PRIMER_RIGHT_21_END_STABILITY=2.5900
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=9.1785
PRIMER_LEFT_22_PENALTY=1.728621
PRIMER_RIGHT_22_PENALTY=2.496898
PRIMER_INTERNAL_22_PENALTY=3.956741
PRIMER_LEFT_22_SEQUENCE=CGATCCCGACCCAAATCGATA
PRIMER_RIGHT_22_SEQUENCE=GTCTGCATCGTCAGTGTCA
PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_LEFT_22=74,21
PRIMER_RIGHT_22=33,19
PRIMER_INTERNAL_22=66,19
PRIMER_LEFT_22_TM=59.729
PRIMER_RIGHT_22_TM=57.503
PRIMER_INTERNAL_22_TM=57.043
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=52.632
PRIMER_INTERNAL_22_GC_PERCENT=68.421
PRIMER_INTERNAL_22_SELF_ANY_TH=2.18
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n             5' GGGCCATTCGATCCCGACC 3'\n                 ||||\n3' CCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=45.13
PRIMER_RIGHT_22_HAIRPIN_TH=35.63
PRIMER_INTERNAL_22_HAIRPIN_TH=41.60
PRIMER_LEFT_22_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' CGATCCCGACCCU+2510\n         |||   U+2502\n   3' ATAGCTAAAU+2518\n
PRIMER_RIGHT_22_HAIRPIN_STUCT=Tm: 35.6&deg;C  dG: 127 cal/mol  dH: -28600 cal/mol  dS: -93 cal/mol*K\n   5' GTCTGCATU+2510\n        |||   U+2502\n3' ACTGTGACTGCU+2518\n
PRIMER_INTERNAL_22_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n     3' CCAGCCCU+2518\n
PRIMER_LEFT_22_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_22_END_STABILITY=2.9200
PRIMER_RIGHT_22_END_STABILITY=3.5800
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=6.6045
PRIMER_LEFT_23_PENALTY=1.914379
PRIMER_RIGHT_23_PENALTY=2.637998
PRIMER_INTERNAL_23_PENALTY=4.001118
PRIMER_LEFT_23_SEQUENCE=CCCGACCCAAATCGATAGC
PRIMER_RIGHT_23_SEQUENCE=TCGTAGCTATCGATTTGGGTC
PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT
PRIMER_LEFT_23=78,19
PRIMER_RIGHT_23=101,21
PRIMER_INTERNAL_23=36,24
PRIMER_LEFT_23_TM=58.086
PRIMER_RIGHT_23_TM=57.362
PRIMER_INTERNAL_23_TM=60.001
PRIMER_LEFT_23_GC_PERCENT=57.895
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_INTERNAL_23_GC_PERCENT=54.167
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=1.71
PRIMER_RIGHT_23_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n5' TCGTAGCTATCGATTTGGGTC 3'\n           ||||||\n 3' CTGGGTTTAGCTATCGATGCT 5'\n
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=45.13
PRIMER_RIGHT_23_HAIRPIN_TH=32.30
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n   5' CCCGACCCU+2510\n        |||   U+2502\n3' CGATAGCTAAAU+2518\n
PRIMER_RIGHT_23_HAIRPIN_STUCT=Tm: 32.3&deg;C  dG: 369 cal/mol  dH: -24000 cal/mol  dS: -79 cal/mol*K\n        5' TCGTAGU+2510\n           |||   C\n3' CTGGGTTTAGCTATU+2518\n
PRIMER_LEFT_23_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_23_END_STABILITY=2.9700
PRIMER_RIGHT_23_END_STABILITY=4.4600
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=3.1819
PRIMER_LEFT_24_PENALTY=1.926077
PRIMER_RIGHT_24_PENALTY=2.695830
PRIMER_INTERNAL_24_PENALTY=4.001118
PRIMER_LEFT_24_SEQUENCE=ATTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_24_SEQUENCE=GGATCGAATGGCCCACTAATA
PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG
PRIMER_LEFT_24=60,21
PRIMER_RIGHT_24=79,21
PRIMER_INTERNAL_24=35,24
PRIMER_LEFT_24_TM=59.926
PRIMER_RIGHT_24_TM=57.304
PRIMER_INTERNAL_24_TM=60.001
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_INTERNAL_24_GC_PERCENT=54.167
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=10.70
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n    5' ATTAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGATTA 5'\n
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=40.47
PRIMER_RIGHT_24_HAIRPIN_TH=0.00
PRIMER_INTERNAL_24_HAIRPIN_TH=0.00
PRIMER_LEFT_24_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' ATTAGTGGGCCATU+2510\n         |||    T\n      3' CCCTAGCU+2518\n
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_24_END_STABILITY=3.8500
PRIMER_RIGHT_24_END_STABILITY=0.9800
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.5023
PRIMER_LEFT_25_PENALTY=1.960819
PRIMER_RIGHT_25_PENALTY=2.754380
PRIMER_INTERNAL_25_PENALTY=4.058186
PRIMER_LEFT_25_SEQUENCE=GATCCCGACCCAAATCGATAG
PRIMER_RIGHT_25_SEQUENCE=CGGGATCGAATGGCCCACTA
PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC
PRIMER_LEFT_25=75,21
PRIMER_RIGHT_25=81,20
PRIMER_INTERNAL_25=67,24
PRIMER_LEFT_25_TM=58.039
PRIMER_RIGHT_25_TM=61.754
PRIMER_INTERNAL_25_TM=59.942
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=60.000
PRIMER_INTERNAL_25_GC_PERCENT=58.333
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=45.13
PRIMER_RIGHT_25_HAIRPIN_TH=42.14
PRIMER_INTERNAL_25_HAIRPIN_TH=41.60
PRIMER_LEFT_25_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' GATCCCGACCCU+2510\n        |||   U+2502\n 3' GATAGCTAAAU+2518\n
PRIMER_RIGHT_25_HAIRPIN_STUCT=Tm: 42.1&deg;C  dG: -373 cal/mol  dH: -22900 cal/mol  dS: -73 cal/mol*K\n   5' CGGGATCGU+2510\n       |||    A\n3' ATCACCCGGTAU+2518\n
PRIMER_INTERNAL_25_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n  5' GGCCATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_LEFT_25_LIBRARY_MISPRIMING=10.00, MSTa consensus sequence
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_25_END_STABILITY=2.0800
PRIMER_RIGHT_25_END_STABILITY=2.7400
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=9.7973
PRIMER_LEFT_26_PENALTY=1.997913
PRIMER_RIGHT_26_PENALTY=2.831715
PRIMER_INTERNAL_26_PENALTY=4.099823
PRIMER_LEFT_26_SEQUENCE=ATCCCGACCCAAATCGATAGC
PRIMER_RIGHT_26_SEQUENCE=TCGTCAGTGTCATGTCTACTG
PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_26=76,21
PRIMER_RIGHT_26=26,21
PRIMER_INTERNAL_26=66,23
PRIMER_LEFT_26_TM=59.998
PRIMER_RIGHT_26_TM=57.168
PRIMER_INTERNAL_26_TM=61.100
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=60.870
PRIMER_INTERNAL_26_SELF_ANY_TH=2.18
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=15.66
PRIMER_RIGHT_26_SELF_ANY_STUCT=Tm: 15.7&deg;C  dG: -3531 cal/mol  dH: -104000 cal/mol  dS: -324 cal/mol*K\n5' TCGTCAGTGT-CATGTCTACTG 3'\n       ||||   ||||   ||||\n    3' GTCATCTGTACTG-TGACTGCT 5'\n
PRIMER_INTERNAL_26_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n                 5' GGGCCATTCGATCCCGACCCAAA 3'\n                     ||||\n3' AAACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=15.66
PRIMER_RIGHT_26_SELF_END_STUCT=Tm: 15.7&deg;C  dG: -3531 cal/mol  dH: -104000 cal/mol  dS: -324 cal/mol*K\n5' TCGTCAGTGT-CATGTCTACTG 3'\n       ||||   ||||   ||||\n    3' GTCATCTGTACTG-TGACTGCT 5'\n
PRIMER_LEFT_26_HAIRPIN_TH=45.13
PRIMER_RIGHT_26_HAIRPIN_TH=42.34
PRIMER_INTERNAL_26_HAIRPIN_TH=41.60
PRIMER_LEFT_26_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n 5' ATCCCGACCCU+2510\n        |||   U+2502\n3' CGATAGCTAAAU+2518\n
PRIMER_RIGHT_26_HAIRPIN_STUCT=Tm: 42.3&deg;C  dG: -552 cal/mol  dH: -32600 cal/mol  dS: -103 cal/mol*K\n5' TCGTCAGTGTCAU+2510\n       ||||    T\n    3' GTCATCTGU+2518\n
PRIMER_INTERNAL_26_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n 3' AAACCCAGCCCU+2518\n
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=2.9700
PRIMER_RIGHT_26_END_STABILITY=2.7400
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=24.9329
PRIMER_LEFT_27_PENALTY=2.038681
PRIMER_RIGHT_27_PENALTY=2.831715
PRIMER_INTERNAL_27_PENALTY=4.159384
PRIMER_LEFT_27_SEQUENCE=TCGATCCCGACCCAAATCGA
PRIMER_RIGHT_27_SEQUENCE=CGTCAGTGTCATGTCTACTGA
PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT
PRIMER_LEFT_27=73,20
PRIMER_RIGHT_27=25,21
PRIMER_INTERNAL_27=38,22
PRIMER_LEFT_27_TM=61.039
PRIMER_RIGHT_27_TM=57.168
PRIMER_INTERNAL_27_TM=57.841
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=54.545
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=2.47
PRIMER_RIGHT_27_SELF_ANY_TH=18.56
PRIMER_LEFT_27_SELF_ANY_STUCT=Tm: 2.5&deg;C  dG: -2545 cal/mol  dH: -69200 cal/mol  dS: -215 cal/mol*K\n5' TCGATCCCGACCCAAATCGA 3'\n   |||||          |||||\n3' AGCTAAACCCAGCCCTAGCT 5'\n
PRIMER_RIGHT_27_SELF_ANY_STUCT=Tm: 18.6&deg;C  dG: -4389 cal/mol  dH: -108000 cal/mol  dS: -334 cal/mol*K\n5' CGTCAGTGT-CATGTCTACTGA 3'\n     |||||   ||||   |||||\n  3' AGTCATCTGTACTG-TGACTGC 5'\n
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=2.47
PRIMER_RIGHT_27_SELF_END_TH=18.56
PRIMER_LEFT_27_SELF_END_STUCT=Tm: 2.5&deg;C  dG: -2545 cal/mol  dH: -69200 cal/mol  dS: -215 cal/mol*K\n5' TCGATCCCGACCCAAATCGA 3'\n   |||||          |||||\n3' AGCTAAACCCAGCCCTAGCT 5'\n
PRIMER_RIGHT_27_SELF_END_STUCT=Tm: 18.6&deg;C  dG: -4389 cal/mol  dH: -108000 cal/mol  dS: -334 cal/mol*K\n5' CGTCAGTGT-CATGTCTACTGA 3'\n     |||||   ||||   |||||\n  3' AGTCATCTGTACTG-TGACTGC 5'\n
PRIMER_LEFT_27_HAIRPIN_TH=45.41
PRIMER_RIGHT_27_HAIRPIN_TH=46.05
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_HAIRPIN_STUCT=Tm: 45.4&deg;C  dG: -916 cal/mol  dH: -34700 cal/mol  dS: -109 cal/mol*K\n5' TCGATCCCGAU+2510\n   |||||     U+2502\n3' AGCTAAACCCU+2518\n
PRIMER_RIGHT_27_HAIRPIN_STUCT=Tm: 46.0&deg;C  dG: -981 cal/mol  dH: -34600 cal/mol  dS: -108 cal/mol*K\n5' CGTCAGTGTCAU+2510\n     |||||    T\n  3' AGTCATCTGU+2518\n
PRIMER_LEFT_27_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER44C Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=3.5900
PRIMER_RIGHT_27_END_STABILITY=3.4100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=26.0802
PRIMER_LEFT_28_PENALTY=2.150848
PRIMER_RIGHT_28_PENALTY=2.912899
PRIMER_INTERNAL_28_PENALTY=4.159384
PRIMER_LEFT_28_SEQUENCE=TAGACATGACACTGACGATGC
PRIMER_RIGHT_28_SEQUENCE=TGCATCGTCAGTGTCATGT
PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG
PRIMER_LEFT_28=9,21
PRIMER_RIGHT_28=30,19
PRIMER_INTERNAL_28=37,22
PRIMER_LEFT_28_TM=57.849
PRIMER_RIGHT_28_TM=57.087
PRIMER_INTERNAL_28_TM=57.841
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.368
PRIMER_INTERNAL_28_GC_PERCENT=54.545
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=0.00
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_28_END_STABILITY=3.9100
PRIMER_RIGHT_28_END_STABILITY=3.2100
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=3.7339
PRIMER_LEFT_29_PENALTY=2.469564
PRIMER_RIGHT_29_PENALTY=2.989104
PRIMER_INTERNAL_29_PENALTY=4.196497
PRIMER_LEFT_29_SEQUENCE=CATTCGATCCCGACCCAAA
PRIMER_RIGHT_29_SEQUENCE=AGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG
PRIMER_LEFT_29=70,19
PRIMER_RIGHT_29=97,19
PRIMER_INTERNAL_29=36,23
PRIMER_LEFT_29_TM=57.530
PRIMER_RIGHT_29_TM=57.011
PRIMER_INTERNAL_29_TM=58.804
PRIMER_LEFT_29_GC_PERCENT=52.632
PRIMER_RIGHT_29_GC_PERCENT=52.632
PRIMER_INTERNAL_29_GC_PERCENT=56.522
PRIMER_INTERNAL_29_SELF_ANY_TH=0.00
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=1.71
PRIMER_RIGHT_29_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n     5' AGCTATCGATTTGGGTCGG 3'\n            ||||||\n3' GGCTGGGTTTAGCTATCGA 5'\n
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=45.50
PRIMER_RIGHT_29_HAIRPIN_TH=41.74
PRIMER_INTERNAL_29_HAIRPIN_TH=0.00
PRIMER_LEFT_29_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n   5' CATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_RIGHT_29_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' AGCTATCGATTTU+2510\n         |||   U+2502\n     3' GGCTGGGU+2518\n
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_29_END_STABILITY=3.2800
PRIMER_RIGHT_29_END_STABILITY=4.7900
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=2.2809
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_primer_list_and_file_flag_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=30
PRIMER_RIGHT_NUM_RETURNED=30
PRIMER_INTERNAL_NUM_RETURNED=30
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_LEFT_0=118,20
PRIMER_RIGHT_0=137,20
PRIMER_INTERNAL_0=125,20
PRIMER_LEFT_0_TM=58.514
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=59.906
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGA 3'\n     ||   |||  |||   ||\n 3' AGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n          3' AGCCCU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_1_PENALTY=1.160593
PRIMER_RIGHT_1_PENALTY=1.160593
PRIMER_INTERNAL_1_PENALTY=0.301069
PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_1=122,19
PRIMER_RIGHT_1=140,19
PRIMER_INTERNAL_1=126,20
PRIMER_LEFT_1_TM=59.161
PRIMER_RIGHT_1_TM=59.161
PRIMER_INTERNAL_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_SELF_ANY_TH=7.82
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n 5' ACGCAAAGCACGCTCC-CGAT 3'\n     ||   |||  |||   ||\n3' TAGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=37.44
PRIMER_INTERNAL_1_HAIRPIN_TH=36.42
PRIMER_RIGHT_1_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n    5' GAGCGTGU+2510\n         ||   C\n3' GTTTTGCGTTTU+2518\n
PRIMER_INTERNAL_1_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n        3' TAGCCCU+2518\n
PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_1_END_STABILITY=5.0300
PRIMER_RIGHT_1_END_STABILITY=2.4400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_2_PENALTY=1.656085
PRIMER_RIGHT_2_PENALTY=1.656085
PRIMER_INTERNAL_2_PENALTY=0.900305
PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA
PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG
PRIMER_LEFT_2=117,21
PRIMER_RIGHT_2=137,21
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=58.344
PRIMER_INTERNAL_2_TM=59.100
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.14
PRIMER_INTERNAL_2_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n            3' GCCCU+2518\n
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=5.3400
PRIMER_RIGHT_2_END_STABILITY=1.7500
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=8.6247
PRIMER_LEFT_3_PENALTY=1.695359
PRIMER_RIGHT_3_PENALTY=1.695359
PRIMER_INTERNAL_3_PENALTY=0.956520
PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC
PRIMER_LEFT_3=53,21
PRIMER_RIGHT_3=73,21
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=58.305
PRIMER_RIGHT_3_TM=58.305
PRIMER_INTERNAL_3_TM=59.043
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=36.42
PRIMER_INTERNAL_3_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n      3' CTAGCCCU+2518\n
PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.7700
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_4_PENALTY=1.831685
PRIMER_RIGHT_4_PENALTY=1.831685
PRIMER_INTERNAL_4_PENALTY=1.018091
PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA
PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT
PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=84,20
PRIMER_INTERNAL_4=125,21
PRIMER_LEFT_4_TM=57.168
PRIMER_RIGHT_4_TM=57.168
PRIMER_INTERNAL_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=57.143
PRIMER_INTERNAL_4_SELF_ANY_TH=7.82
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGAT 3'\n     ||   |||  |||   ||\n3' TAGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=35.45
PRIMER_RIGHT_4_HAIRPIN_TH=31.80
PRIMER_INTERNAL_4_HAIRPIN_TH=36.42
PRIMER_LEFT_4_HAIRPIN_STUCT=Tm: 35.4&deg;C  dG: 103 cal/mol  dH: -20400 cal/mol  dS: -66 cal/mol*K\n5' AACGCCAACTCACTU+2510\n            |||  G\n         3' AGTATU+2518\n
PRIMER_RIGHT_4_HAIRPIN_STUCT=Tm: 31.8&deg;C  dG: 377 cal/mol  dH: -22100 cal/mol  dS: -72 cal/mol*K\n         5' TCATAU+2510\n            |||  C\n3' TTGCGGTTGAGTGAU+2518\n
PRIMER_INTERNAL_4_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n         3' TAGCCCU+2518\n
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=2.1500
PRIMER_RIGHT_4_END_STABILITY=4.8400
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.8143
PRIMER_LEFT_5_PENALTY=1.902705
PRIMER_RIGHT_5_PENALTY=1.902705
PRIMER_INTERNAL_5_PENALTY=1.178696
PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA
PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_5=62,20
PRIMER_RIGHT_5=81,20
PRIMER_INTERNAL_5=124,21
PRIMER_LEFT_5_TM=57.097
PRIMER_RIGHT_5_TM=57.097
PRIMER_INTERNAL_5_TM=60.179
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=57.143
PRIMER_INTERNAL_5_SELF_ANY_TH=7.82
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGA 3'\n      ||   |||  |||   ||\n  3' AGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=36.42
PRIMER_INTERNAL_5_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n           3' AGCCCU+2518\n
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=2.7400
PRIMER_RIGHT_5_END_STABILITY=2.5200
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=29.7752
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_6_PENALTY=1.902705
PRIMER_RIGHT_6_PENALTY=1.902705
PRIMER_INTERNAL_6_PENALTY=1.372876
PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT
PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA
PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_6=61,20
PRIMER_RIGHT_6=80,20
PRIMER_INTERNAL_6=126,21
PRIMER_LEFT_6_TM=57.097
PRIMER_RIGHT_6_TM=57.097
PRIMER_INTERNAL_6_TM=60.373
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=7.82
PRIMER_LEFT_6_SELF_ANY_TH=0.00
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n  5' ACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=36.42
PRIMER_INTERNAL_6_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n       3' CTAGCCCU+2518\n
PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=3.5500
PRIMER_RIGHT_6_END_STABILITY=2.1000
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.0753
PRIMER_LEFT_7_PENALTY=1.950877
PRIMER_RIGHT_7_PENALTY=1.950877
PRIMER_INTERNAL_7_PENALTY=1.393730
PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG
PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA
PRIMER_LEFT_7=119,19
PRIMER_RIGHT_7=137,19
PRIMER_INTERNAL_7=126,19
PRIMER_LEFT_7_TM=58.049
PRIMER_RIGHT_7_TM=58.049
PRIMER_INTERNAL_7_TM=59.606
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_INTERNAL_7_GC_PERCENT=63.158
PRIMER_INTERNAL_7_SELF_ANY_TH=7.82
PRIMER_LEFT_7_SELF_ANY_TH=0.00
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' ACGCAAAGCACGCTCC-CGA 3'\n    ||   |||  |||   ||\n3' AGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=0.00
PRIMER_RIGHT_7_HAIRPIN_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=36.42
PRIMER_INTERNAL_7_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n         3' AGCCCU+2518\n
PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=5.3400
PRIMER_RIGHT_7_END_STABILITY=3.5300
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_8_PENALTY=1.972625
PRIMER_RIGHT_8_PENALTY=1.972625
PRIMER_INTERNAL_8_PENALTY=1.595076
PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA
PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_8=56,21
PRIMER_RIGHT_8=76,21
PRIMER_INTERNAL_8=123,21
PRIMER_LEFT_8_TM=58.027
PRIMER_RIGHT_8_TM=58.027
PRIMER_INTERNAL_8_TM=59.405
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_INTERNAL_8_GC_PERCENT=57.143
PRIMER_INTERNAL_8_SELF_ANY_TH=0.00
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=0.00
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=41.14
PRIMER_INTERNAL_8_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n             3' GCCCU+2518\n
PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_LEFT_8_END_STABILITY=3.4100
PRIMER_RIGHT_8_END_STABILITY=3.8600
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=32.2237
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.8066
PRIMER_LEFT_9_PENALTY=1.973945
PRIMER_RIGHT_9_PENALTY=1.973945
PRIMER_INTERNAL_9_PENALTY=2.007622
PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC
PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT
PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_9=55,21
PRIMER_RIGHT_9=75,21
PRIMER_INTERNAL_9=121,22
PRIMER_LEFT_9_TM=58.026
PRIMER_RIGHT_9_TM=58.026
PRIMER_INTERNAL_9_TM=59.992
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_INTERNAL_9_GC_PERCENT=59.091
PRIMER_INTERNAL_9_SELF_ANY_TH=0.00
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=0.00
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=0.00
PRIMER_RIGHT_9_HAIRPIN_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=0.00
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=3.0100
PRIMER_RIGHT_9_END_STABILITY=4.0000
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=27.4392
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=5.5040
PRIMER_LEFT_10_PENALTY=1.973945
PRIMER_RIGHT_10_PENALTY=1.973945
PRIMER_INTERNAL_10_PENALTY=2.235780
PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT
PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC
PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG
PRIMER_LEFT_10=54,21
PRIMER_RIGHT_10=74,21
PRIMER_INTERNAL_10=125,19
PRIMER_LEFT_10_TM=58.026
PRIMER_RIGHT_10_TM=58.026
PRIMER_INTERNAL_10_TM=58.764
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_INTERNAL_10_GC_PERCENT=63.158
PRIMER_INTERNAL_10_SELF_ANY_TH=0.00
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=0.00
PRIMER_RIGHT_10_HAIRPIN_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.14
PRIMER_INTERNAL_10_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n           3' GCCCU+2518\n
PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=3.1600
PRIMER_RIGHT_10_END_STABILITY=3.8500
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=19.5725
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=9.9844
PRIMER_LEFT_11_PENALTY=2.120321
PRIMER_RIGHT_11_PENALTY=2.120321
PRIMER_INTERNAL_11_PENALTY=2.240074
PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA
PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT
PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_11=64,21
PRIMER_RIGHT_11=84,21
PRIMER_INTERNAL_11=124,22
PRIMER_LEFT_11_TM=57.880
PRIMER_RIGHT_11_TM=57.880
PRIMER_INTERNAL_11_TM=60.240
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_INTERNAL_11_GC_PERCENT=54.545
PRIMER_INTERNAL_11_SELF_ANY_TH=7.82
PRIMER_LEFT_11_SELF_ANY_TH=0.00
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGAT 3'\n      ||   |||  |||   ||\n 3' TAGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=35.45
PRIMER_RIGHT_11_HAIRPIN_TH=31.80
PRIMER_INTERNAL_11_HAIRPIN_TH=36.42
PRIMER_LEFT_11_HAIRPIN_STUCT=Tm: 35.4&deg;C  dG: 103 cal/mol  dH: -20400 cal/mol  dS: -66 cal/mol*K\n5' AAACGCCAACTCACTU+2510\n             |||  G\n          3' AGTATU+2518\n
PRIMER_RIGHT_11_HAIRPIN_STUCT=Tm: 31.8&deg;C  dG: 377 cal/mol  dH: -22100 cal/mol  dS: -72 cal/mol*K\n          5' TCATAU+2510\n             |||  C\n3' TTTGCGGTTGAGTGAU+2518\n
PRIMER_INTERNAL_11_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n          3' TAGCCCU+2518\n
PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=2.1500
PRIMER_RIGHT_11_END_STABILITY=3.6000
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8905
PRIMER_LEFT_12_PENALTY=2.142778
PRIMER_RIGHT_12_PENALTY=2.142778
PRIMER_INTERNAL_12_PENALTY=2.299276
PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_12=119,20
PRIMER_RIGHT_12=138,20
PRIMER_INTERNAL_12=122,22
PRIMER_LEFT_12_TM=61.143
PRIMER_RIGHT_12_TM=61.143
PRIMER_INTERNAL_12_TM=60.299
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=59.091
PRIMER_INTERNAL_12_SELF_ANY_TH=0.00
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=0.00
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.14
PRIMER_INTERNAL_12_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' CAAAACGCAAAGCACGCTU+2510\n                 ||  U+2502\n              3' GCCCU+2518\n
PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=5.3400
PRIMER_RIGHT_12_END_STABILITY=3.5300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_13_PENALTY=2.299089
PRIMER_RIGHT_13_PENALTY=2.299089
PRIMER_INTERNAL_13_PENALTY=2.427391
PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC
PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_13=123,19
PRIMER_RIGHT_13=141,19
PRIMER_INTERNAL_13=123,22
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.299
PRIMER_INTERNAL_13_TM=60.427
PRIMER_LEFT_13_GC_PERCENT=52.632
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_INTERNAL_13_GC_PERCENT=54.545
PRIMER_INTERNAL_13_SELF_ANY_TH=7.82
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=0.00
PRIMER_INTERNAL_13_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGA 3'\n       ||   |||  |||   ||\n   3' AGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=0.00
PRIMER_RIGHT_13_HAIRPIN_TH=37.44
PRIMER_INTERNAL_13_HAIRPIN_TH=36.42
PRIMER_RIGHT_13_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n  5' GGAGCGTGU+2510\n        ||   C\n3' TTTTGCGTTTU+2518\n
PRIMER_INTERNAL_13_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n            3' AGCCCU+2518\n
PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_13_END_STABILITY=4.7000
PRIMER_RIGHT_13_END_STABILITY=2.4300
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_14_PENALTY=2.402628
PRIMER_RIGHT_14_PENALTY=2.402628
PRIMER_INTERNAL_14_PENALTY=2.610811
PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT
PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_14=62,21
PRIMER_RIGHT_14=82,21
PRIMER_INTERNAL_14=125,22
PRIMER_LEFT_14_TM=57.597
PRIMER_RIGHT_14_TM=57.597
PRIMER_INTERNAL_14_TM=60.611
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_INTERNAL_14_GC_PERCENT=59.091
PRIMER_INTERNAL_14_SELF_ANY_TH=7.82
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n 5' AACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=0.00
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=0.00
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=36.42
PRIMER_INTERNAL_14_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n        3' CTAGCCCU+2518\n
PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=2.2900
PRIMER_RIGHT_14_END_STABILITY=2.5200
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=30.4432
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_15_PENALTY=2.402628
PRIMER_RIGHT_15_PENALTY=2.402628
PRIMER_INTERNAL_15_PENALTY=2.740095
PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT
PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT
PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT
PRIMER_LEFT_15=60,21
PRIMER_RIGHT_15=80,21
PRIMER_INTERNAL_15=127,19
PRIMER_LEFT_15_TM=57.597
PRIMER_RIGHT_15_TM=57.597
PRIMER_INTERNAL_15_TM=58.260
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_INTERNAL_15_GC_PERCENT=63.158
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=0.00
PRIMER_RIGHT_15_HAIRPIN_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=36.42
PRIMER_INTERNAL_15_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n       3' TAGCCCU+2518\n
PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=3.5500
PRIMER_RIGHT_15_END_STABILITY=1.9800
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0593
PRIMER_LEFT_16_PENALTY=2.455619
PRIMER_RIGHT_16_PENALTY=2.455619
PRIMER_INTERNAL_16_PENALTY=2.880381
PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG
PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC
PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_16=63,21
PRIMER_RIGHT_16=83,21
PRIMER_INTERNAL_16=120,22
PRIMER_LEFT_16_TM=57.544
PRIMER_RIGHT_16_TM=57.544
PRIMER_INTERNAL_16_TM=59.120
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_INTERNAL_16_GC_PERCENT=54.545
PRIMER_INTERNAL_16_SELF_ANY_TH=0.00
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=0.00
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=0.00
PRIMER_RIGHT_16_HAIRPIN_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=0.00
PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_LEFT_16_END_STABILITY=2.3900
PRIMER_RIGHT_16_END_STABILITY=2.7800
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=25.0463
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8601
PRIMER_LEFT_17_PENALTY=2.539776
PRIMER_RIGHT_17_PENALTY=2.539776
PRIMER_INTERNAL_17_PENALTY=2.939429
PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC
PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_17=122,20
PRIMER_RIGHT_17=141,20
PRIMER_INTERNAL_17=122,21
PRIMER_LEFT_17_TM=61.540
PRIMER_RIGHT_17_TM=61.540
PRIMER_INTERNAL_17_TM=58.061
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_TH=37.44
PRIMER_INTERNAL_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n   5' GGAGCGTGU+2510\n         ||   C\n3' GTTTTGCGTTTU+2518\n
PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_17_END_STABILITY=4.7000
PRIMER_RIGHT_17_END_STABILITY=2.4400
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_18_PENALTY=2.539776
PRIMER_RIGHT_18_PENALTY=2.539776
PRIMER_INTERNAL_18_PENALTY=2.939429
PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_18=121,20
PRIMER_RIGHT_18=140,20
PRIMER_INTERNAL_18=121,21
PRIMER_LEFT_18_TM=61.540
PRIMER_RIGHT_18_TM=61.540
PRIMER_INTERNAL_18_TM=58.061
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_INTERNAL_18_GC_PERCENT=57.143
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_TH=37.44
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n     5' GAGCGTGU+2510\n          ||   C\n3' GGTTTTGCGTTTU+2518\n
PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_18_END_STABILITY=5.0300
PRIMER_RIGHT_18_END_STABILITY=3.2800
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_19_PENALTY=2.587613
PRIMER_RIGHT_19_PENALTY=2.587613
PRIMER_INTERNAL_19_PENALTY=3.476553
PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC
PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_19=120,19
PRIMER_RIGHT_19=138,19
PRIMER_INTERNAL_19=123,23
PRIMER_LEFT_19_TM=60.588
PRIMER_RIGHT_19_TM=60.588
PRIMER_INTERNAL_19_TM=60.477
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_INTERNAL_19_GC_PERCENT=52.174
PRIMER_INTERNAL_19_SELF_ANY_TH=7.82
PRIMER_LEFT_19_SELF_ANY_TH=0.00
PRIMER_RIGHT_19_SELF_ANY_TH=0.00
PRIMER_INTERNAL_19_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGAT 3'\n       ||   |||  |||   ||\n  3' TAGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=36.42
PRIMER_INTERNAL_19_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n           3' TAGCCCU+2518\n
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_19_END_STABILITY=5.3400
PRIMER_RIGHT_19_END_STABILITY=3.5300
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=2.588667
PRIMER_RIGHT_20_PENALTY=2.588667
PRIMER_INTERNAL_20_PENALTY=3.598972
PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT
PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_20=121,19
PRIMER_RIGHT_20=139,19
PRIMER_INTERNAL_20=119,23
PRIMER_LEFT_20_TM=60.589
PRIMER_RIGHT_20_TM=60.589
PRIMER_INTERNAL_20_TM=59.401
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=52.632
PRIMER_INTERNAL_20_GC_PERCENT=52.174
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=0.00
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=0.00
PRIMER_LEFT_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_TH=37.44
PRIMER_INTERNAL_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n      5' AGCGTGU+2510\n          ||   C\n3' GGTTTTGCGTTTU+2518\n
PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_20_END_STABILITY=5.0700
PRIMER_RIGHT_20_END_STABILITY=3.2800
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_21_PENALTY=2.627395
PRIMER_RIGHT_21_PENALTY=2.627395
PRIMER_INTERNAL_21_PENALTY=3.606233
PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA
PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT
PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG
PRIMER_LEFT_21=52,21
PRIMER_RIGHT_21=72,21
PRIMER_INTERNAL_21=126,18
PRIMER_LEFT_21_TM=57.373
PRIMER_RIGHT_21_TM=57.373
PRIMER_INTERNAL_21_TM=58.394
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_INTERNAL_21_GC_PERCENT=66.667
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.14
PRIMER_INTERNAL_21_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n          3' GCCCU+2518\n
PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_21_END_STABILITY=4.5200
PRIMER_RIGHT_21_END_STABILITY=3.0600
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=3.9584
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_22_PENALTY=2.678056
PRIMER_RIGHT_22_PENALTY=2.678056
PRIMER_INTERNAL_22_PENALTY=3.829005
PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT
PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA
PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_22=58,21
PRIMER_RIGHT_22=78,21
PRIMER_INTERNAL_22=124,23
PRIMER_LEFT_22_TM=57.322
PRIMER_RIGHT_22_TM=57.322
PRIMER_INTERNAL_22_TM=60.829
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_INTERNAL_22_GC_PERCENT=56.522
PRIMER_INTERNAL_22_SELF_ANY_TH=7.82
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=0.00
PRIMER_RIGHT_22_HAIRPIN_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=36.42
PRIMER_INTERNAL_22_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n         3' CTAGCCCU+2518\n
PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_22_END_STABILITY=3.4100
PRIMER_RIGHT_22_END_STABILITY=2.1500
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=25.2513
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=19.0855
PRIMER_LEFT_23_PENALTY=2.780624
PRIMER_RIGHT_23_PENALTY=2.780624
PRIMER_INTERNAL_23_PENALTY=3.865064
PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT
PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_23=62,19
PRIMER_RIGHT_23=80,19
PRIMER_INTERNAL_23=120,21
PRIMER_LEFT_23_TM=57.219
PRIMER_RIGHT_23_TM=57.219
PRIMER_INTERNAL_23_TM=57.135
PRIMER_LEFT_23_GC_PERCENT=47.368
PRIMER_RIGHT_23_GC_PERCENT=47.368
PRIMER_INTERNAL_23_GC_PERCENT=52.381
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=0.00
PRIMER_RIGHT_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_23_END_STABILITY=3.5500
PRIMER_RIGHT_23_END_STABILITY=2.5200
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_24_PENALTY=2.999071
PRIMER_RIGHT_24_PENALTY=2.999071
PRIMER_INTERNAL_24_PENALTY=3.905453
PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA
PRIMER_LEFT_24=120,20
PRIMER_RIGHT_24=139,20
PRIMER_INTERNAL_24=127,18
PRIMER_LEFT_24_TM=61.999
PRIMER_RIGHT_24_TM=61.999
PRIMER_INTERNAL_24_TM=58.095
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_INTERNAL_24_GC_PERCENT=66.667
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=0.00
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=0.00
PRIMER_RIGHT_24_HAIRPIN_TH=37.44
PRIMER_INTERNAL_24_HAIRPIN_TH=36.42
PRIMER_RIGHT_24_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n       5' AGCGTGU+2510\n           ||   C\n3' AGGTTTTGCGTTTU+2518\n
PRIMER_INTERNAL_24_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n        3' AGCCCU+2518\n
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_24_END_STABILITY=5.0700
PRIMER_RIGHT_24_END_STABILITY=3.5300
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_25_PENALTY=2.999229
PRIMER_RIGHT_25_PENALTY=2.999229
PRIMER_INTERNAL_25_PENALTY=3.954555
PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG
PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC
PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_25=59,21
PRIMER_RIGHT_25=79,21
PRIMER_INTERNAL_25=120,23
PRIMER_LEFT_25_TM=57.001
PRIMER_RIGHT_25_TM=57.001
PRIMER_INTERNAL_25_TM=60.955
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_INTERNAL_25_GC_PERCENT=56.522
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=0.00
PRIMER_RIGHT_25_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=0.00
PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_25_END_STABILITY=3.6600
PRIMER_RIGHT_25_END_STABILITY=2.0800
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=30.7683
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=20.4326
PRIMER_LEFT_26_PENALTY=2.999229
PRIMER_RIGHT_26_PENALTY=2.999229
PRIMER_INTERNAL_26_PENALTY=4.240075
PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC
PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG
PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_26=57,21
PRIMER_RIGHT_26=77,21
PRIMER_INTERNAL_26=122,23
PRIMER_LEFT_26_TM=57.001
PRIMER_RIGHT_26_TM=57.001
PRIMER_INTERNAL_26_TM=61.240
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=56.522
PRIMER_INTERNAL_26_SELF_ANY_TH=7.82
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=0.00
PRIMER_INTERNAL_26_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' CAAAACGCAAAGCACGCTCC-CGA 3'\n        ||   |||  |||   ||\n    3' AGCCC-TCGCACGAAACGCAAAAC 5'\n
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=0.00
PRIMER_LEFT_26_HAIRPIN_TH=0.00
PRIMER_RIGHT_26_HAIRPIN_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=36.42
PRIMER_INTERNAL_26_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CAAAACGCAAAGCACGCTU+2510\n                 ||  U+2502\n             3' AGCCCU+2518\n
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=3.5100
PRIMER_RIGHT_26_END_STABILITY=2.9000
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=20.2809
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.6247
PRIMER_LEFT_27_PENALTY=3.455227
PRIMER_RIGHT_27_PENALTY=3.455227
PRIMER_INTERNAL_27_PENALTY=4.486709
PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_27=118,21
PRIMER_RIGHT_27=138,21
PRIMER_INTERNAL_27=119,22
PRIMER_LEFT_27_TM=61.455
PRIMER_RIGHT_27_TM=61.455
PRIMER_INTERNAL_27_TM=57.513
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=50.000
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=0.00
PRIMER_RIGHT_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=0.00
PRIMER_RIGHT_27_SELF_END_TH=0.00
PRIMER_LEFT_27_HAIRPIN_TH=0.00
PRIMER_RIGHT_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=5.3400
PRIMER_RIGHT_27_END_STABILITY=3.0100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_28_PENALTY=3.677573
PRIMER_RIGHT_28_PENALTY=3.677573
PRIMER_INTERNAL_28_PENALTY=4.513970
PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC
PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_28=123,20
PRIMER_RIGHT_28=142,20
PRIMER_INTERNAL_28=118,24
PRIMER_LEFT_28_TM=62.678
PRIMER_RIGHT_28_TM=62.678
PRIMER_INTERNAL_28_TM=59.486
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_INTERNAL_28_GC_PERCENT=50.000
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=37.44
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n 5' GGGAGCGTGU+2510\n        ||   C\n3' TTTTGCGTTTU+2518\n
PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_28_END_STABILITY=4.3000
PRIMER_RIGHT_28_END_STABILITY=2.4300
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.1346
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_29_PENALTY=4.100862
PRIMER_RIGHT_29_PENALTY=4.100862
PRIMER_INTERNAL_29_PENALTY=5.029817
PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC
PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC
PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_29=128,19
PRIMER_RIGHT_29=146,19
PRIMER_INTERNAL_29=123,24
PRIMER_LEFT_29_TM=62.101
PRIMER_RIGHT_29_TM=62.101
PRIMER_INTERNAL_29_TM=61.030
PRIMER_LEFT_29_GC_PERCENT=63.158
PRIMER_RIGHT_29_GC_PERCENT=63.158
PRIMER_INTERNAL_29_GC_PERCENT=54.167
PRIMER_INTERNAL_29_SELF_ANY_TH=7.82
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGATC 3'\n       ||   |||  |||   ||\n 3' CTAGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=38.29
PRIMER_RIGHT_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=36.42
PRIMER_LEFT_29_HAIRPIN_STUCT=Tm: 38.3&deg;C  dG: -93 cal/mol  dH: -22500 cal/mol  dS: -72 cal/mol*K\n5' GCAAAGCACGCTU+2510\n           ||  U+2502\n     3' CTAGCCCU+2518\n
PRIMER_INTERNAL_29_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n          3' CTAGCCCU+2518\n
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_29_END_STABILITY=3.6900
PRIMER_RIGHT_29_END_STABILITY=3.6800
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0362
=
PRIMER_TASK=pick_primer_list
PRIMER_PRODUCT_SIZE_RANGE=75-200
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=4
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=4
SEQUENCE_ID=pick_primer_list_and_file_flag_th_3
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52
PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 17, high repeat similarity 3, ok 34
PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, high hairpin stability 29, ok 168
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=30
PRIMER_RIGHT_NUM_RETURNED=30
PRIMER_INTERNAL_NUM_RETURNED=30
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.527529
PRIMER_RIGHT_0_PENALTY=0.527529
PRIMER_INTERNAL_0_PENALTY=0.727038
PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA
PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=80,20
PRIMER_INTERNAL_0=66,20
PRIMER_LEFT_0_TM=59.528
PRIMER_RIGHT_0_TM=59.528
PRIMER_INTERNAL_0_TM=59.273
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=2.18
PRIMER_LEFT_0_SELF_ANY_TH=10.70
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n     5' TTAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGATT 5'\n
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n              5' GGGCCATTCGATCCCGACCC 3'\n                  ||||\n3' CCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=40.47
PRIMER_RIGHT_0_HAIRPIN_TH=41.64
PRIMER_INTERNAL_0_HAIRPIN_TH=41.60
PRIMER_LEFT_0_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' TTAGTGGGCCATU+2510\n        |||    T\n     3' CCCTAGCU+2518\n
PRIMER_RIGHT_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n     5' GGGATCGU+2510\n        |||    A\n3' AATCACCCGGTAU+2518\n
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n    3' CCCAGCCCU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_RIGHT_0_END_STABILITY=2.2400
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266
PRIMER_LEFT_1_PENALTY=0.606923
PRIMER_RIGHT_1_PENALTY=0.606923
PRIMER_INTERNAL_1_PENALTY=1.416589
PRIMER_LEFT_1_SEQUENCE=CCCGACCCAAATCGATAGCT
PRIMER_RIGHT_1_SEQUENCE=AGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC
PRIMER_LEFT_1=78,20
PRIMER_RIGHT_1=97,20
PRIMER_INTERNAL_1=64,21
PRIMER_LEFT_1_TM=59.607
PRIMER_RIGHT_1_TM=59.607
PRIMER_INTERNAL_1_TM=59.583
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_SELF_ANY_TH=7.03
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=1.71
PRIMER_RIGHT_1_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n      5' AGCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCGA 5'\n
PRIMER_INTERNAL_1_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n            5' GTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.13
PRIMER_RIGHT_1_HAIRPIN_TH=41.74
PRIMER_INTERNAL_1_HAIRPIN_TH=41.60
PRIMER_LEFT_1_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n    5' CCCGACCCU+2510\n         |||   U+2502\n3' TCGATAGCTAAAU+2518\n
PRIMER_RIGHT_1_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' AGCTATCGATTTU+2510\n         |||   U+2502\n    3' GGGCTGGGU+2518\n
PRIMER_INTERNAL_1_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n       3' CCAGCCCU+2518\n
PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_1_END_STABILITY=3.3200
PRIMER_RIGHT_1_END_STABILITY=5.1400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=11.3993
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_2_PENALTY=0.608403
PRIMER_RIGHT_2_PENALTY=0.608403
PRIMER_INTERNAL_2_PENALTY=1.438741
PRIMER_LEFT_2_SEQUENCE=TCCCGACCCAAATCGATAGC
PRIMER_RIGHT_2_SEQUENCE=GCTATCGATTTGGGTCGGGA
PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA
PRIMER_LEFT_2=77,20
PRIMER_RIGHT_2=96,20
PRIMER_INTERNAL_2=67,20
PRIMER_LEFT_2_TM=59.608
PRIMER_RIGHT_2_TM=59.608
PRIMER_INTERNAL_2_TM=58.561
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=1.71
PRIMER_RIGHT_2_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n        5' GCTATCGATTTGGGTCGGGA 3'\n              ||||||\n3' AGGGCTGGGTTTAGCTATCG 5'\n
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=45.13
PRIMER_RIGHT_2_HAIRPIN_TH=41.74
PRIMER_INTERNAL_2_HAIRPIN_TH=41.60
PRIMER_LEFT_2_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n  5' TCCCGACCCU+2510\n        |||   U+2502\n3' CGATAGCTAAAU+2518\n
PRIMER_RIGHT_2_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GCTATCGATTTU+2510\n        |||   U+2502\n  3' AGGGCTGGGU+2518\n
PRIMER_INTERNAL_2_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n  3' ACCCAGCCCU+2518\n
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_2_END_STABILITY=2.9700
PRIMER_RIGHT_2_END_STABILITY=5.1400
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=2.4428
PRIMER_LEFT_3_PENALTY=0.713597
PRIMER_RIGHT_3_PENALTY=0.713597
PRIMER_INTERNAL_3_PENALTY=1.438741
PRIMER_LEFT_3_SEQUENCE=GACATGACACTGACGATGCA
PRIMER_RIGHT_3_SEQUENCE=TGCATCGTCAGTGTCATGTC
PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC
PRIMER_LEFT_3=11,20
PRIMER_RIGHT_3=30,20
PRIMER_INTERNAL_3=65,20
PRIMER_LEFT_3_TM=58.286
PRIMER_RIGHT_3_TM=58.286
PRIMER_INTERNAL_3_TM=58.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=41.60
PRIMER_INTERNAL_3_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TGGGCCATTCGATU+2510\n           |||  U+2502\n      3' CCAGCCCU+2518\n
PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_3_END_STABILITY=3.9600
PRIMER_RIGHT_3_END_STABILITY=3.0600
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=16.7219
PRIMER_LEFT_4_PENALTY=0.966496
PRIMER_RIGHT_4_PENALTY=0.966496
PRIMER_INTERNAL_4_PENALTY=1.650306
PRIMER_LEFT_4_SEQUENCE=CGATCCCGACCCAAATCGAT
PRIMER_RIGHT_4_SEQUENCE=ATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA
PRIMER_LEFT_4=74,20
PRIMER_RIGHT_4=93,20
PRIMER_INTERNAL_4=66,21
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.966
PRIMER_INTERNAL_4_TM=60.650
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_INTERNAL_4_SELF_ANY_TH=2.18
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n               5' GGGCCATTCGATCCCGACCCA 3'\n                   ||||\n3' ACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=45.13
PRIMER_RIGHT_4_HAIRPIN_TH=41.74
PRIMER_INTERNAL_4_HAIRPIN_TH=41.60
PRIMER_LEFT_4_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' CGATCCCGACCCU+2510\n         |||   U+2502\n    3' TAGCTAAAU+2518\n
PRIMER_RIGHT_4_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n    5' ATCGATTTU+2510\n         |||   U+2502\n3' GCTAGGGCTGGGU+2518\n
PRIMER_INTERNAL_4_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n   3' ACCCAGCCCU+2518\n
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_4_END_STABILITY=3.5900
PRIMER_RIGHT_4_END_STABILITY=3.6900
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_5_PENALTY=0.967319
PRIMER_RIGHT_5_PENALTY=1.001812
PRIMER_INTERNAL_5_PENALTY=1.650306
PRIMER_LEFT_5_SEQUENCE=CATTCGATCCCGACCCAAAT
PRIMER_RIGHT_5_SEQUENCE=GTCTGCATCGTCAGTGTCAT
PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC
PRIMER_LEFT_5=70,20
PRIMER_RIGHT_5=33,20
PRIMER_INTERNAL_5=65,21
PRIMER_LEFT_5_TM=58.033
PRIMER_RIGHT_5_TM=57.998
PRIMER_INTERNAL_5_TM=60.650
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_INTERNAL_5_GC_PERCENT=66.667
PRIMER_INTERNAL_5_SELF_ANY_TH=0.00
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=45.50
PRIMER_RIGHT_5_HAIRPIN_TH=35.63
PRIMER_INTERNAL_5_HAIRPIN_TH=41.60
PRIMER_LEFT_5_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n    5' CATTCGATU+2510\n          |||  U+2502\n3' TAAACCCAGCCCU+2518\n
PRIMER_RIGHT_5_HAIRPIN_STUCT=Tm: 35.6&deg;C  dG: 127 cal/mol  dH: -28600 cal/mol  dS: -93 cal/mol*K\n    5' GTCTGCATU+2510\n         |||   U+2502\n3' TACTGTGACTGCU+2518\n
PRIMER_INTERNAL_5_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TGGGCCATTCGATU+2510\n           |||  U+2502\n     3' CCCAGCCCU+2518\n
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_5_END_STABILITY=2.3200
PRIMER_RIGHT_5_END_STABILITY=3.0600
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=8.6039
PRIMER_LEFT_6_PENALTY=1.000429
PRIMER_RIGHT_6_PENALTY=1.001812
PRIMER_INTERNAL_6_PENALTY=2.095978
PRIMER_LEFT_6_SEQUENCE=CATGACACTGACGATGCAGA
PRIMER_RIGHT_6_SEQUENCE=GCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA
PRIMER_LEFT_6=13,20
PRIMER_RIGHT_6=29,20
PRIMER_INTERNAL_6=67,21
PRIMER_LEFT_6_TM=58.000
PRIMER_RIGHT_6_TM=57.998
PRIMER_INTERNAL_6_TM=58.904
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=0.00
PRIMER_LEFT_6_SELF_ANY_TH=10.65
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_LEFT_6_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -3380 cal/mol  dH: -84400 cal/mol  dS: -261 cal/mol*K\n5' CATGACACTGA--CGATGCAGA 3'\n          |||   ||   |||\n      3' AGACGTAGCA--GTCACAGTAC 5'\n
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=39.20
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=41.60
PRIMER_LEFT_6_HAIRPIN_STUCT=Tm: 39.2&deg;C  dG: -189 cal/mol  dH: -26900 cal/mol  dS: -86 cal/mol*K\n5' CATGACACTGACGU+2510\n          |||   U+2502\n      3' AGACGTAU+2518\n
PRIMER_INTERNAL_6_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n 3' AACCCAGCCCU+2518\n
PRIMER_LEFT_6_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_6_END_STABILITY=4.2600
PRIMER_RIGHT_6_END_STABILITY=3.4100
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=1.2632
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_7_PENALTY=1.001812
PRIMER_RIGHT_7_PENALTY=1.142220
PRIMER_INTERNAL_7_PENALTY=2.360266
PRIMER_LEFT_7_SEQUENCE=ATGACACTGACGATGCAGAC
PRIMER_RIGHT_7_SEQUENCE=GGGATCGAATGGCCCACTA
PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_7=14,20
PRIMER_RIGHT_7=80,19
PRIMER_INTERNAL_7=63,21
PRIMER_LEFT_7_TM=57.998
PRIMER_RIGHT_7_TM=58.858
PRIMER_INTERNAL_7_TM=58.640
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=57.895
PRIMER_INTERNAL_7_GC_PERCENT=61.905
PRIMER_INTERNAL_7_SELF_ANY_TH=7.03
PRIMER_LEFT_7_SELF_ANY_TH=10.65
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_LEFT_7_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -3380 cal/mol  dH: -84400 cal/mol  dS: -261 cal/mol*K\n5' ATGACACTGA--CGATGCAGAC 3'\n         |||   ||   |||\n    3' CAGACGTAGCA--GTCACAGTA 5'\n
PRIMER_INTERNAL_7_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n          5' AGTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=39.20
PRIMER_RIGHT_7_HAIRPIN_TH=41.64
PRIMER_INTERNAL_7_HAIRPIN_TH=41.60
PRIMER_LEFT_7_HAIRPIN_STUCT=Tm: 39.2&deg;C  dG: -189 cal/mol  dH: -26900 cal/mol  dS: -86 cal/mol*K\n5' ATGACACTGACGU+2510\n         |||   U+2502\n    3' CAGACGTAU+2518\n
PRIMER_RIGHT_7_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n    5' GGGATCGU+2510\n       |||    A\n3' ATCACCCGGTAU+2518\n
PRIMER_INTERNAL_7_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' AGTGGGCCATTCGATU+2510\n             |||  U+2502\n         3' CAGCCCU+2518\n
PRIMER_LEFT_7_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_7_END_STABILITY=3.5100
PRIMER_RIGHT_7_END_STABILITY=2.7400
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=16.1639
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=7.8928
PRIMER_LEFT_8_PENALTY=1.001812
PRIMER_RIGHT_8_PENALTY=1.316792
PRIMER_INTERNAL_8_PENALTY=2.456420
PRIMER_LEFT_8_SEQUENCE=AGACATGACACTGACGATGC
PRIMER_RIGHT_8_SEQUENCE=CGTAGCTATCGATTTGGGTCG
PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA
PRIMER_LEFT_8=10,20
PRIMER_RIGHT_8=100,21
PRIMER_INTERNAL_8=63,20
PRIMER_LEFT_8_TM=57.998
PRIMER_RIGHT_8_TM=58.683
PRIMER_INTERNAL_8_TM=57.544
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_INTERNAL_8_GC_PERCENT=60.000
PRIMER_INTERNAL_8_SELF_ANY_TH=7.03
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=1.71
PRIMER_RIGHT_8_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n 5' CGTAGCTATCGATTTGGGTCG 3'\n           ||||||\n3' GCTGGGTTTAGCTATCGATGC 5'\n
PRIMER_INTERNAL_8_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n         5' AGTGGGCCATTCGATCCCGA 3'\n              ||| |||\n3' AGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=42.74
PRIMER_INTERNAL_8_HAIRPIN_TH=39.49
PRIMER_RIGHT_8_HAIRPIN_STUCT=Tm: 42.7&deg;C  dG: -478 cal/mol  dH: -26300 cal/mol  dS: -83 cal/mol*K\n5' CGTAGCTATCGATTTU+2510\n            |||   U+2502\n         3' GCTGGGU+2518\n
PRIMER_INTERNAL_8_HAIRPIN_STUCT=Tm: 39.5&deg;C  dG: -197 cal/mol  dH: -24700 cal/mol  dS: -79 cal/mol*K\n5' AGTGGGCCATU+2510\n      |||    T\n 3' AGCCCTAGCU+2518\n
PRIMER_LEFT_8_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_8_END_STABILITY=3.9100
PRIMER_RIGHT_8_END_STABILITY=4.7900
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=7.5573
PRIMER_LEFT_9_PENALTY=1.107410
PRIMER_RIGHT_9_PENALTY=1.383135
PRIMER_INTERNAL_9_PENALTY=2.511372
PRIMER_LEFT_9_SEQUENCE=CCATTCGATCCCGACCCAAA
PRIMER_RIGHT_9_SEQUENCE=TAGCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC
PRIMER_LEFT_9=69,20
PRIMER_RIGHT_9=98,21
PRIMER_INTERNAL_9=64,20
PRIMER_LEFT_9_TM=60.107
PRIMER_RIGHT_9_TM=59.383
PRIMER_INTERNAL_9_TM=57.489
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_INTERNAL_9_GC_PERCENT=65.000
PRIMER_INTERNAL_9_SELF_ANY_TH=7.03
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=1.71
PRIMER_RIGHT_9_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n     5' TAGCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCGAT 5'\n
PRIMER_INTERNAL_9_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n           5' GTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=45.50
PRIMER_RIGHT_9_HAIRPIN_TH=41.74
PRIMER_INTERNAL_9_HAIRPIN_TH=41.60
PRIMER_LEFT_9_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n  5' CCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_RIGHT_9_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' TAGCTATCGATTTU+2510\n          |||   U+2502\n     3' GGGCTGGGU+2518\n
PRIMER_INTERNAL_9_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n        3' CAGCCCU+2518\n
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_9_END_STABILITY=3.2800
PRIMER_RIGHT_9_END_STABILITY=5.1400
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_10_PENALTY=1.126409
PRIMER_RIGHT_10_PENALTY=1.559940
PRIMER_INTERNAL_10_PENALTY=2.626619
PRIMER_LEFT_10_SEQUENCE=CATTCGATCCCGACCCAAATC
PRIMER_RIGHT_10_SEQUENCE=TCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_10=70,21
PRIMER_RIGHT_10=92,19
PRIMER_INTERNAL_10=63,22
PRIMER_LEFT_10_TM=59.126
PRIMER_RIGHT_10_TM=59.560
PRIMER_INTERNAL_10_TM=60.627
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=57.895
PRIMER_INTERNAL_10_GC_PERCENT=63.636
PRIMER_INTERNAL_10_SELF_ANY_TH=7.03
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n           5' AGTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTGA 5'\n
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=45.50
PRIMER_RIGHT_10_HAIRPIN_TH=41.74
PRIMER_INTERNAL_10_HAIRPIN_TH=41.60
PRIMER_LEFT_10_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n     5' CATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_RIGHT_10_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n     5' TCGATTTU+2510\n         |||   U+2502\n3' GCTAGGGCTGGGU+2518\n
PRIMER_INTERNAL_10_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' AGTGGGCCATTCGATU+2510\n             |||  U+2502\n        3' CCAGCCCU+2518\n
PRIMER_LEFT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_10_END_STABILITY=2.1700
PRIMER_RIGHT_10_END_STABILITY=3.6900
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_11_PENALTY=1.142220
PRIMER_RIGHT_11_PENALTY=1.559940
PRIMER_INTERNAL_11_PENALTY=2.783921
PRIMER_LEFT_11_SEQUENCE=TAGTGGGCCATTCGATCCC
PRIMER_RIGHT_11_SEQUENCE=CGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_11=62,19
PRIMER_RIGHT_11=91,19
PRIMER_INTERNAL_11=67,22
PRIMER_LEFT_11_TM=58.858
PRIMER_RIGHT_11_TM=59.560
PRIMER_INTERNAL_11_TM=59.216
PRIMER_LEFT_11_GC_PERCENT=57.895
PRIMER_RIGHT_11_GC_PERCENT=57.895
PRIMER_INTERNAL_11_GC_PERCENT=59.091
PRIMER_INTERNAL_11_SELF_ANY_TH=0.00
PRIMER_LEFT_11_SELF_ANY_TH=10.70
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_LEFT_11_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n      5' TAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=40.47
PRIMER_RIGHT_11_HAIRPIN_TH=42.82
PRIMER_INTERNAL_11_HAIRPIN_TH=41.60
PRIMER_LEFT_11_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' TAGTGGGCCATU+2510\n       |||    T\n    3' CCCTAGCU+2518\n
PRIMER_RIGHT_11_HAIRPIN_STUCT=Tm: 42.8&deg;C  dG: -584 cal/mol  dH: -31700 cal/mol  dS: -100 cal/mol*K\n 5' CGATTTGGGU+2510\n    ||||     U+2502\n3' AGCTAGGGCTU+2518\n
PRIMER_INTERNAL_11_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_LEFT_11_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_11_END_STABILITY=3.8500
PRIMER_RIGHT_11_END_STABILITY=3.5900
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_12_PENALTY=1.179094
PRIMER_RIGHT_12_PENALTY=1.568623
PRIMER_INTERNAL_12_PENALTY=2.885177
PRIMER_LEFT_12_SEQUENCE=TTCGATCCCGACCCAAATCG
PRIMER_RIGHT_12_SEQUENCE=GGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA
PRIMER_LEFT_12=72,20
PRIMER_RIGHT_12=79,20
PRIMER_INTERNAL_12=66,22
PRIMER_LEFT_12_TM=60.179
PRIMER_RIGHT_12_TM=57.431
PRIMER_INTERNAL_12_TM=60.885
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=63.636
PRIMER_INTERNAL_12_SELF_ANY_TH=2.18
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n                5' GGGCCATTCGATCCCGACCCAA 3'\n                    ||||\n3' AACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=45.50
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.60
PRIMER_LEFT_12_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n        5' TTCGATU+2510\n            |||  U+2502\n3' GCTAAACCCAGCCCU+2518\n
PRIMER_INTERNAL_12_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n  3' AACCCAGCCCU+2518\n
PRIMER_LEFT_12_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_12_END_STABILITY=3.3400
PRIMER_RIGHT_12_END_STABILITY=1.7300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=2.4855
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=4.2003
PRIMER_LEFT_13_PENALTY=1.256117
PRIMER_RIGHT_13_PENALTY=1.673833
PRIMER_INTERNAL_13_PENALTY=3.114431
PRIMER_LEFT_13_SEQUENCE=ATTCGATCCCGACCCAAATC
PRIMER_RIGHT_13_SEQUENCE=GTAGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC
PRIMER_LEFT_13=71,20
PRIMER_RIGHT_13=99,21
PRIMER_INTERNAL_13=62,23
PRIMER_LEFT_13_TM=57.744
PRIMER_RIGHT_13_TM=58.326
PRIMER_INTERNAL_13_TM=60.114
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_INTERNAL_13_GC_PERCENT=60.870
PRIMER_INTERNAL_13_SELF_ANY_TH=7.03
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=1.71
PRIMER_RIGHT_13_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n   5' GTAGCTATCGATTTGGGTCGG 3'\n            ||||||\n3' GGCTGGGTTTAGCTATCGATG 5'\n
PRIMER_INTERNAL_13_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n          5' TAGTGGGCCATTCGATCCCGACC 3'\n                ||| |||\n3' CCAGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=45.50
PRIMER_RIGHT_13_HAIRPIN_TH=41.74
PRIMER_INTERNAL_13_HAIRPIN_TH=41.60
PRIMER_LEFT_13_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n      5' ATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_RIGHT_13_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GTAGCTATCGATTTU+2510\n           |||   U+2502\n       3' GGCTGGGU+2518\n
PRIMER_INTERNAL_13_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TAGTGGGCCATTCGATU+2510\n              |||  U+2502\n         3' CCAGCCCU+2518\n
PRIMER_LEFT_13_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_13_END_STABILITY=2.1700
PRIMER_RIGHT_13_END_STABILITY=4.7900
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=2.2809
PRIMER_LEFT_14_PENALTY=1.316792
PRIMER_RIGHT_14_PENALTY=1.726673
PRIMER_INTERNAL_14_PENALTY=3.510595
PRIMER_LEFT_14_SEQUENCE=CGACCCAAATCGATAGCTACG
PRIMER_RIGHT_14_SEQUENCE=TGCATCGTCAGTGTCATGTCT
PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC
PRIMER_LEFT_14=80,21
PRIMER_RIGHT_14=30,21
PRIMER_INTERNAL_14=64,22
PRIMER_LEFT_14_TM=58.683
PRIMER_RIGHT_14_TM=59.727
PRIMER_INTERNAL_14_TM=61.511
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_INTERNAL_14_GC_PERCENT=68.182
PRIMER_INTERNAL_14_SELF_ANY_TH=7.03
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n             5' GTGGGCCATTCGATCCCGACCC 3'\n                 ||| |||\n3' CCCAGCCCTAGCTTACCGGGTG 5'\n
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=2.30
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_STUCT=Tm: 2.3&deg;C  dG: -3380 cal/mol  dH: -62200 cal/mol  dS: -190 cal/mol*K\n5' CGACCCAAATCGATAGCTA-CG 3'\n                |||||| ||\n           3' GCATCGATAGCTAAACCCAGC 5'\n
PRIMER_LEFT_14_HAIRPIN_TH=45.13
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=41.60
PRIMER_LEFT_14_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n         5' CGACCCU+2510\n            |||   U+2502\n3' GCATCGATAGCTAAAU+2518\n
PRIMER_INTERNAL_14_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GTGGGCCATTCGATU+2510\n            |||  U+2502\n      3' CCCAGCCCU+2518\n
PRIMER_LEFT_14_LIBRARY_MISPRIMING=10.00, MER4A a consensus
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_14_END_STABILITY=3.5100
PRIMER_RIGHT_14_END_STABILITY=3.4100
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5113
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=15.2988
PRIMER_LEFT_15_PENALTY=1.383135
PRIMER_RIGHT_15_PENALTY=1.728621
PRIMER_INTERNAL_15_PENALTY=3.685489
PRIMER_LEFT_15_SEQUENCE=CCCGACCCAAATCGATAGCTA
PRIMER_RIGHT_15_SEQUENCE=TATCGATTTGGGTCGGGATCG
PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT
PRIMER_LEFT_15=78,21
PRIMER_RIGHT_15=94,21
PRIMER_INTERNAL_15=67,23
PRIMER_LEFT_15_TM=59.383
PRIMER_RIGHT_15_TM=59.729
PRIMER_INTERNAL_15_TM=59.315
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_INTERNAL_15_GC_PERCENT=56.522
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=1.71
PRIMER_RIGHT_15_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n             5' TATCGATTTGGGTCGGGATCG 3'\n                 ||||||\n3' GCTAGGGCTGGGTTTAGCTAT 5'\n
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=45.13
PRIMER_RIGHT_15_HAIRPIN_TH=41.74
PRIMER_INTERNAL_15_HAIRPIN_TH=41.60
PRIMER_LEFT_15_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n     5' CCCGACCCU+2510\n          |||   U+2502\n3' ATCGATAGCTAAAU+2518\n
PRIMER_RIGHT_15_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n   5' TATCGATTTU+2510\n         |||   U+2502\n3' GCTAGGGCTGGGU+2518\n
PRIMER_INTERNAL_15_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' GGCCATTCGATU+2510\n          |||  U+2502\n3' TAAACCCAGCCCU+2518\n
PRIMER_LEFT_15_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_15_END_STABILITY=3.3200
PRIMER_RIGHT_15_END_STABILITY=3.6900
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=13.2273
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=11.6783
PRIMER_LEFT_16_PENALTY=1.475039
PRIMER_RIGHT_16_PENALTY=1.914379
PRIMER_INTERNAL_16_PENALTY=3.807618
PRIMER_LEFT_16_SEQUENCE=CCATTCGATCCCGACCCAA
PRIMER_RIGHT_16_SEQUENCE=GCTATCGATTTGGGTCGGG
PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC
PRIMER_LEFT_16=69,19
PRIMER_RIGHT_16=96,19
PRIMER_INTERNAL_16=62,22
PRIMER_LEFT_16_TM=59.475
PRIMER_RIGHT_16_TM=58.086
PRIMER_INTERNAL_16_TM=58.192
PRIMER_LEFT_16_GC_PERCENT=57.895
PRIMER_RIGHT_16_GC_PERCENT=57.895
PRIMER_INTERNAL_16_GC_PERCENT=59.091
PRIMER_INTERNAL_16_SELF_ANY_TH=7.03
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=1.71
PRIMER_RIGHT_16_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n       5' GCTATCGATTTGGGTCGGG 3'\n             ||||||\n3' GGGCTGGGTTTAGCTATCG 5'\n
PRIMER_INTERNAL_16_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n         5' TAGTGGGCCATTCGATCCCGAC 3'\n               ||| |||\n3' CAGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=45.50
PRIMER_RIGHT_16_HAIRPIN_TH=41.74
PRIMER_INTERNAL_16_HAIRPIN_TH=41.60
PRIMER_LEFT_16_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' CCATTCGATU+2510\n        |||  U+2502\n3' AACCCAGCCCU+2518\n
PRIMER_RIGHT_16_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GCTATCGATTTU+2510\n        |||   U+2502\n   3' GGGCTGGGU+2518\n
PRIMER_INTERNAL_16_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' TAGTGGGCCATTCGATU+2510\n              |||  U+2502\n          3' CAGCCCU+2518\n
PRIMER_LEFT_16_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_LEFT_16_END_STABILITY=4.1200
PRIMER_RIGHT_16_END_STABILITY=5.1400
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=1.9633
PRIMER_LEFT_17_PENALTY=1.559940
PRIMER_RIGHT_17_PENALTY=1.926077
PRIMER_INTERNAL_17_PENALTY=3.826170
PRIMER_LEFT_17_SEQUENCE=CGATCCCGACCCAAATCGA
PRIMER_RIGHT_17_SEQUENCE=GGGATCGAATGGCCCACTAAT
PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA
PRIMER_LEFT_17=74,19
PRIMER_RIGHT_17=80,21
PRIMER_INTERNAL_17=68,21
PRIMER_LEFT_17_TM=59.560
PRIMER_RIGHT_17_TM=59.926
PRIMER_INTERNAL_17_TM=57.174
PRIMER_LEFT_17_GC_PERCENT=57.895
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=45.13
PRIMER_RIGHT_17_HAIRPIN_TH=41.64
PRIMER_INTERNAL_17_HAIRPIN_TH=41.60
PRIMER_LEFT_17_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' CGATCCCGACCCU+2510\n         |||   U+2502\n     3' AGCTAAAU+2518\n
PRIMER_RIGHT_17_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -386 cal/mol  dH: -26200 cal/mol  dS: -83 cal/mol*K\n      5' GGGATCGU+2510\n         |||    A\n3' TAATCACCCGGTAU+2518\n
PRIMER_INTERNAL_17_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n 5' GCCATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_LEFT_17_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_17_END_STABILITY=3.5900
PRIMER_RIGHT_17_END_STABILITY=1.7300
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=12.3668
PRIMER_LEFT_18_PENALTY=1.559940
PRIMER_RIGHT_18_PENALTY=1.960819
PRIMER_INTERNAL_18_PENALTY=3.864865
PRIMER_LEFT_18_SEQUENCE=TCGATCCCGACCCAAATCG
PRIMER_RIGHT_18_SEQUENCE=CTATCGATTTGGGTCGGGATC
PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT
PRIMER_LEFT_18=73,19
PRIMER_RIGHT_18=95,21
PRIMER_INTERNAL_18=37,23
PRIMER_LEFT_18_TM=59.560
PRIMER_RIGHT_18_TM=58.039
PRIMER_INTERNAL_18_TM=59.135
PRIMER_LEFT_18_GC_PERCENT=57.895
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_INTERNAL_18_GC_PERCENT=52.174
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=1.71
PRIMER_RIGHT_18_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n           5' CTATCGATTTGGGTCGGGATC 3'\n                ||||||\n3' CTAGGGCTGGGTTTAGCTATC 5'\n
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=40.30
PRIMER_RIGHT_18_HAIRPIN_TH=41.74
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_LEFT_18_HAIRPIN_STUCT=Tm: 40.3&deg;C  dG: -227 cal/mol  dH: -21600 cal/mol  dS: -69 cal/mol*K\n         5' TCGATU+2510\n            |||  U+2502\n3' GCTAAACCCAGCCCU+2518\n
PRIMER_RIGHT_18_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n 5' CTATCGATTTU+2510\n        |||   U+2502\n3' CTAGGGCTGGGU+2518\n
PRIMER_LEFT_18_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_18_END_STABILITY=3.3400
PRIMER_RIGHT_18_END_STABILITY=3.3600
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=0.0363
PRIMER_LEFT_19_PENALTY=1.568623
PRIMER_RIGHT_19_PENALTY=1.997913
PRIMER_INTERNAL_19_PENALTY=3.881423
PRIMER_LEFT_19_SEQUENCE=ATTAGTGGGCCATTCGATCC
PRIMER_RIGHT_19_SEQUENCE=GCTATCGATTTGGGTCGGGAT
PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA
PRIMER_LEFT_19=60,20
PRIMER_RIGHT_19=96,21
PRIMER_INTERNAL_19=62,21
PRIMER_LEFT_19_TM=57.431
PRIMER_RIGHT_19_TM=59.998
PRIMER_INTERNAL_19_TM=57.119
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_INTERNAL_19_GC_PERCENT=57.143
PRIMER_INTERNAL_19_SELF_ANY_TH=7.03
PRIMER_LEFT_19_SELF_ANY_TH=10.70
PRIMER_RIGHT_19_SELF_ANY_TH=1.71
PRIMER_LEFT_19_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n   5' ATTAGTGGGCCATTCGATCC 3'\n           ||| |||\n3' CCTAGCTTACCGGGTGATTA 5'\n
PRIMER_RIGHT_19_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n         5' GCTATCGATTTGGGTCGGGAT 3'\n               ||||||\n3' TAGGGCTGGGTTTAGCTATCG 5'\n
PRIMER_INTERNAL_19_SELF_ANY_STUCT=Tm: 7.0&deg;C  dG: -6146 cal/mol  dH: -47400 cal/mol  dS: -133 cal/mol*K\n        5' TAGTGGGCCATTCGATCCCGA 3'\n              ||| |||\n3' AGCCCTAGCTTACCGGGTGAT 5'\n
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=41.74
PRIMER_INTERNAL_19_HAIRPIN_TH=39.49
PRIMER_RIGHT_19_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' GCTATCGATTTU+2510\n        |||   U+2502\n 3' TAGGGCTGGGU+2518\n
PRIMER_INTERNAL_19_HAIRPIN_STUCT=Tm: 39.5&deg;C  dG: -197 cal/mol  dH: -24700 cal/mol  dS: -79 cal/mol*K\n5' TAGTGGGCCATU+2510\n       |||    T\n  3' AGCCCTAGCU+2518\n
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_19_END_STABILITY=3.3600
PRIMER_RIGHT_19_END_STABILITY=3.8500
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=9.4710
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=1.673833
PRIMER_RIGHT_20_PENALTY=2.038681
PRIMER_INTERNAL_20_PENALTY=3.936624
PRIMER_LEFT_20_SEQUENCE=CCGACCCAAATCGATAGCTAC
PRIMER_RIGHT_20_SEQUENCE=TCGATTTGGGTCGGGATCGA
PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA
PRIMER_LEFT_20=79,21
PRIMER_RIGHT_20=92,20
PRIMER_INTERNAL_20=65,22
PRIMER_LEFT_20_TM=58.326
PRIMER_RIGHT_20_TM=61.039
PRIMER_INTERNAL_20_TM=61.937
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_INTERNAL_20_GC_PERCENT=63.636
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=3.47
PRIMER_RIGHT_20_SELF_ANY_STUCT=Tm: 3.5&deg;C  dG: -2609 cal/mol  dH: -71000 cal/mol  dS: -221 cal/mol*K\n5' TCGATTTGGGTCGGGATCGA 3'\n   |||||          |||||\n3' AGCTAGGGCTGGGTTTAGCT 5'\n
PRIMER_INTERNAL_20_SELF_END_TH=3.99
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=3.47
PRIMER_RIGHT_20_SELF_END_STUCT=Tm: 3.5&deg;C  dG: -2609 cal/mol  dH: -71000 cal/mol  dS: -221 cal/mol*K\n5' TCGATTTGGGTCGGGATCGA 3'\n   |||||          |||||\n3' AGCTAGGGCTGGGTTTAGCT 5'\n
PRIMER_INTERNAL_20_SELF_END_STUCT=Tm: 4.0&deg;C  dG: -782 cal/mol  dH: -87600 cal/mol  dS: -280 cal/mol*K\n5' TGGGCCATTCGATCCCGA----CCCA 3'\n   ||||    |||    |||    ||||\n3' ACCC----AGCCCTAGCTTACCGGGT 5'\n
PRIMER_LEFT_20_HAIRPIN_TH=45.13
PRIMER_RIGHT_20_HAIRPIN_TH=45.48
PRIMER_INTERNAL_20_HAIRPIN_TH=42.67
PRIMER_LEFT_20_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n       5' CCGACCCU+2510\n           |||   U+2502\n3' CATCGATAGCTAAAU+2518\n
PRIMER_RIGHT_20_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -948 cal/mol  dH: -35600 cal/mol  dS: -112 cal/mol*K\n5' TCGATTTGGGU+2510\n   |||||     U+2502\n3' AGCTAGGGCTU+2518\n
PRIMER_INTERNAL_20_HAIRPIN_STUCT=Tm: 42.7&deg;C  dG: -788 cal/mol  dH: -43900 cal/mol  dS: -139 cal/mol*K\n5' TGGGCCATTCGATU+2510\n   ||||    |||  U+2502\n    3' ACCCAGCCCU+2518\n
PRIMER_LEFT_20_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence
PRIMER_LEFT_20_END_STABILITY=3.5800
PRIMER_RIGHT_20_END_STABILITY=3.5900
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.3824
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=4.2576
PRIMER_LEFT_21_PENALTY=1.726673
PRIMER_RIGHT_21_PENALTY=2.150848
PRIMER_INTERNAL_21_PENALTY=3.956741
PRIMER_LEFT_21_SEQUENCE=AGACATGACACTGACGATGCA
PRIMER_RIGHT_21_SEQUENCE=GCATCGTCAGTGTCATGTCTA
PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC
PRIMER_LEFT_21=10,21
PRIMER_RIGHT_21=29,21
PRIMER_INTERNAL_21=67,19
PRIMER_LEFT_21_TM=59.727
PRIMER_RIGHT_21_TM=57.849
PRIMER_INTERNAL_21_TM=57.043
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_INTERNAL_21_GC_PERCENT=68.421
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.60
PRIMER_INTERNAL_21_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGCCATTCGATU+2510\n         |||  U+2502\n   3' CCCAGCCCU+2518\n
PRIMER_LEFT_21_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_21_END_STABILITY=3.9600
PRIMER_RIGHT_21_END_STABILITY=2.5900
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=6.9291
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=9.1785
PRIMER_LEFT_22_PENALTY=1.728621
PRIMER_RIGHT_22_PENALTY=2.496898
PRIMER_INTERNAL_22_PENALTY=3.956741
PRIMER_LEFT_22_SEQUENCE=CGATCCCGACCCAAATCGATA
PRIMER_RIGHT_22_SEQUENCE=GTCTGCATCGTCAGTGTCA
PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC
PRIMER_LEFT_22=74,21
PRIMER_RIGHT_22=33,19
PRIMER_INTERNAL_22=66,19
PRIMER_LEFT_22_TM=59.729
PRIMER_RIGHT_22_TM=57.503
PRIMER_INTERNAL_22_TM=57.043
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=52.632
PRIMER_INTERNAL_22_GC_PERCENT=68.421
PRIMER_INTERNAL_22_SELF_ANY_TH=2.18
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n             5' GGGCCATTCGATCCCGACC 3'\n                 ||||\n3' CCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=45.13
PRIMER_RIGHT_22_HAIRPIN_TH=35.63
PRIMER_INTERNAL_22_HAIRPIN_TH=41.60
PRIMER_LEFT_22_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' CGATCCCGACCCU+2510\n         |||   U+2502\n   3' ATAGCTAAAU+2518\n
PRIMER_RIGHT_22_HAIRPIN_STUCT=Tm: 35.6&deg;C  dG: 127 cal/mol  dH: -28600 cal/mol  dS: -93 cal/mol*K\n   5' GTCTGCATU+2510\n        |||   U+2502\n3' ACTGTGACTGCU+2518\n
PRIMER_INTERNAL_22_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n     3' CCAGCCCU+2518\n
PRIMER_LEFT_22_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_22_END_STABILITY=2.9200
PRIMER_RIGHT_22_END_STABILITY=3.5800
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=6.6045
PRIMER_LEFT_23_PENALTY=1.914379
PRIMER_RIGHT_23_PENALTY=2.637998
PRIMER_INTERNAL_23_PENALTY=4.001118
PRIMER_LEFT_23_SEQUENCE=CCCGACCCAAATCGATAGC
PRIMER_RIGHT_23_SEQUENCE=TCGTAGCTATCGATTTGGGTC
PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT
PRIMER_LEFT_23=78,19
PRIMER_RIGHT_23=101,21
PRIMER_INTERNAL_23=36,24
PRIMER_LEFT_23_TM=58.086
PRIMER_RIGHT_23_TM=57.362
PRIMER_INTERNAL_23_TM=60.001
PRIMER_LEFT_23_GC_PERCENT=57.895
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_INTERNAL_23_GC_PERCENT=54.167
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=1.71
PRIMER_RIGHT_23_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n5' TCGTAGCTATCGATTTGGGTC 3'\n           ||||||\n 3' CTGGGTTTAGCTATCGATGCT 5'\n
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=45.13
PRIMER_RIGHT_23_HAIRPIN_TH=32.30
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n   5' CCCGACCCU+2510\n        |||   U+2502\n3' CGATAGCTAAAU+2518\n
PRIMER_RIGHT_23_HAIRPIN_STUCT=Tm: 32.3&deg;C  dG: 369 cal/mol  dH: -24000 cal/mol  dS: -79 cal/mol*K\n        5' TCGTAGU+2510\n           |||   C\n3' CTGGGTTTAGCTATU+2518\n
PRIMER_LEFT_23_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=11.00, MER4A a consensus
PRIMER_LEFT_23_END_STABILITY=2.9700
PRIMER_RIGHT_23_END_STABILITY=4.4600
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=3.1819
PRIMER_LEFT_24_PENALTY=1.926077
PRIMER_RIGHT_24_PENALTY=2.695830
PRIMER_INTERNAL_24_PENALTY=4.001118
PRIMER_LEFT_24_SEQUENCE=ATTAGTGGGCCATTCGATCCC
PRIMER_RIGHT_24_SEQUENCE=GGATCGAATGGCCCACTAATA
PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG
PRIMER_LEFT_24=60,21
PRIMER_RIGHT_24=79,21
PRIMER_INTERNAL_24=35,24
PRIMER_LEFT_24_TM=59.926
PRIMER_RIGHT_24_TM=57.304
PRIMER_INTERNAL_24_TM=60.001
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_INTERNAL_24_GC_PERCENT=54.167
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=10.70
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_STUCT=Tm: 10.7&deg;C  dG: -6824 cal/mol  dH: -47400 cal/mol  dS: -131 cal/mol*K\n    5' ATTAGTGGGCCATTCGATCCC 3'\n            ||| |||\n3' CCCTAGCTTACCGGGTGATTA 5'\n
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=40.47
PRIMER_RIGHT_24_HAIRPIN_TH=0.00
PRIMER_INTERNAL_24_HAIRPIN_TH=0.00
PRIMER_LEFT_24_HAIRPIN_STUCT=Tm: 40.5&deg;C  dG: -285 cal/mol  dH: -25800 cal/mol  dS: -82 cal/mol*K\n5' ATTAGTGGGCCATU+2510\n         |||    T\n      3' CCCTAGCU+2518\n
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA
PRIMER_LEFT_24_END_STABILITY=3.8500
PRIMER_RIGHT_24_END_STABILITY=0.9800
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=4.1902
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.5023
PRIMER_LEFT_25_PENALTY=1.960819
PRIMER_RIGHT_25_PENALTY=2.754380
PRIMER_INTERNAL_25_PENALTY=4.058186
PRIMER_LEFT_25_SEQUENCE=GATCCCGACCCAAATCGATAG
PRIMER_RIGHT_25_SEQUENCE=CGGGATCGAATGGCCCACTA
PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC
PRIMER_LEFT_25=75,21
PRIMER_RIGHT_25=81,20
PRIMER_INTERNAL_25=67,24
PRIMER_LEFT_25_TM=58.039
PRIMER_RIGHT_25_TM=61.754
PRIMER_INTERNAL_25_TM=59.942
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=60.000
PRIMER_INTERNAL_25_GC_PERCENT=58.333
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=45.13
PRIMER_RIGHT_25_HAIRPIN_TH=42.14
PRIMER_INTERNAL_25_HAIRPIN_TH=41.60
PRIMER_LEFT_25_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n5' GATCCCGACCCU+2510\n        |||   U+2502\n 3' GATAGCTAAAU+2518\n
PRIMER_RIGHT_25_HAIRPIN_STUCT=Tm: 42.1&deg;C  dG: -373 cal/mol  dH: -22900 cal/mol  dS: -73 cal/mol*K\n   5' CGGGATCGU+2510\n       |||    A\n3' ATCACCCGGTAU+2518\n
PRIMER_INTERNAL_25_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n  5' GGCCATTCGATU+2510\n           |||  U+2502\n3' CTAAACCCAGCCCU+2518\n
PRIMER_LEFT_25_LIBRARY_MISPRIMING=10.00, MSTa consensus sequence
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA
PRIMER_LEFT_25_END_STABILITY=2.0800
PRIMER_RIGHT_25_END_STABILITY=2.7400
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=9.7973
PRIMER_LEFT_26_PENALTY=1.997913
PRIMER_RIGHT_26_PENALTY=2.831715
PRIMER_INTERNAL_26_PENALTY=4.099823
PRIMER_LEFT_26_SEQUENCE=ATCCCGACCCAAATCGATAGC
PRIMER_RIGHT_26_SEQUENCE=TCGTCAGTGTCATGTCTACTG
PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA
PRIMER_LEFT_26=76,21
PRIMER_RIGHT_26=26,21
PRIMER_INTERNAL_26=66,23
PRIMER_LEFT_26_TM=59.998
PRIMER_RIGHT_26_TM=57.168
PRIMER_INTERNAL_26_TM=61.100
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=60.870
PRIMER_INTERNAL_26_SELF_ANY_TH=2.18
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=15.66
PRIMER_RIGHT_26_SELF_ANY_STUCT=Tm: 15.7&deg;C  dG: -3531 cal/mol  dH: -104000 cal/mol  dS: -324 cal/mol*K\n5' TCGTCAGTGT-CATGTCTACTG 3'\n       ||||   ||||   ||||\n    3' GTCATCTGTACTG-TGACTGCT 5'\n
PRIMER_INTERNAL_26_SELF_ANY_STUCT=Tm: 2.2&deg;C  dG: -5499 cal/mol  dH: -45200 cal/mol  dS: -128 cal/mol*K\n                 5' GGGCCATTCGATCCCGACCCAAA 3'\n                     ||||\n3' AAACCCAGCCCTAGCTTACCGGG 5'\n
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=15.66
PRIMER_RIGHT_26_SELF_END_STUCT=Tm: 15.7&deg;C  dG: -3531 cal/mol  dH: -104000 cal/mol  dS: -324 cal/mol*K\n5' TCGTCAGTGT-CATGTCTACTG 3'\n       ||||   ||||   ||||\n    3' GTCATCTGTACTG-TGACTGCT 5'\n
PRIMER_LEFT_26_HAIRPIN_TH=45.13
PRIMER_RIGHT_26_HAIRPIN_TH=42.34
PRIMER_INTERNAL_26_HAIRPIN_TH=41.60
PRIMER_LEFT_26_HAIRPIN_STUCT=Tm: 45.1&deg;C  dG: -580 cal/mol  dH: -22700 cal/mol  dS: -71 cal/mol*K\n 5' ATCCCGACCCU+2510\n        |||   U+2502\n3' CGATAGCTAAAU+2518\n
PRIMER_RIGHT_26_HAIRPIN_STUCT=Tm: 42.3&deg;C  dG: -552 cal/mol  dH: -32600 cal/mol  dS: -103 cal/mol*K\n5' TCGTCAGTGTCAU+2510\n       ||||    T\n    3' GTCATCTGU+2518\n
PRIMER_INTERNAL_26_HAIRPIN_STUCT=Tm: 41.6&deg;C  dG: -329 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n5' GGGCCATTCGATU+2510\n          |||  U+2502\n 3' AAACCCAGCCCU+2518\n
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=2.9700
PRIMER_RIGHT_26_END_STABILITY=2.7400
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=3.6843
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=24.9329
PRIMER_LEFT_27_PENALTY=2.038681
PRIMER_RIGHT_27_PENALTY=2.831715
PRIMER_INTERNAL_27_PENALTY=4.159384
PRIMER_LEFT_27_SEQUENCE=TCGATCCCGACCCAAATCGA
PRIMER_RIGHT_27_SEQUENCE=CGTCAGTGTCATGTCTACTGA
PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT
PRIMER_LEFT_27=73,20
PRIMER_RIGHT_27=25,21
PRIMER_INTERNAL_27=38,22
PRIMER_LEFT_27_TM=61.039
PRIMER_RIGHT_27_TM=57.168
PRIMER_INTERNAL_27_TM=57.841
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=54.545
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=2.47
PRIMER_RIGHT_27_SELF_ANY_TH=18.56
PRIMER_LEFT_27_SELF_ANY_STUCT=Tm: 2.5&deg;C  dG: -2545 cal/mol  dH: -69200 cal/mol  dS: -215 cal/mol*K\n5' TCGATCCCGACCCAAATCGA 3'\n   |||||          |||||\n3' AGCTAAACCCAGCCCTAGCT 5'\n
PRIMER_RIGHT_27_SELF_ANY_STUCT=Tm: 18.6&deg;C  dG: -4389 cal/mol  dH: -108000 cal/mol  dS: -334 cal/mol*K\n5' CGTCAGTGT-CATGTCTACTGA 3'\n     |||||   ||||   |||||\n  3' AGTCATCTGTACTG-TGACTGC 5'\n
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=2.47
PRIMER_RIGHT_27_SELF_END_TH=18.56
PRIMER_LEFT_27_SELF_END_STUCT=Tm: 2.5&deg;C  dG: -2545 cal/mol  dH: -69200 cal/mol  dS: -215 cal/mol*K\n5' TCGATCCCGACCCAAATCGA 3'\n   |||||          |||||\n3' AGCTAAACCCAGCCCTAGCT 5'\n
PRIMER_RIGHT_27_SELF_END_STUCT=Tm: 18.6&deg;C  dG: -4389 cal/mol  dH: -108000 cal/mol  dS: -334 cal/mol*K\n5' CGTCAGTGT-CATGTCTACTGA 3'\n     |||||   ||||   |||||\n  3' AGTCATCTGTACTG-TGACTGC 5'\n
PRIMER_LEFT_27_HAIRPIN_TH=45.41
PRIMER_RIGHT_27_HAIRPIN_TH=46.05
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_HAIRPIN_STUCT=Tm: 45.4&deg;C  dG: -916 cal/mol  dH: -34700 cal/mol  dS: -109 cal/mol*K\n5' TCGATCCCGAU+2510\n   |||||     U+2502\n3' AGCTAAACCCU+2518\n
PRIMER_RIGHT_27_HAIRPIN_STUCT=Tm: 46.0&deg;C  dG: -981 cal/mol  dH: -34600 cal/mol  dS: -108 cal/mol*K\n5' CGTCAGTGTCAU+2510\n     |||||    T\n  3' AGTCATCTGU+2518\n
PRIMER_LEFT_27_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER44C Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=3.5900
PRIMER_RIGHT_27_END_STABILITY=3.4100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=26.0802
PRIMER_LEFT_28_PENALTY=2.150848
PRIMER_RIGHT_28_PENALTY=2.912899
PRIMER_INTERNAL_28_PENALTY=4.159384
PRIMER_LEFT_28_SEQUENCE=TAGACATGACACTGACGATGC
PRIMER_RIGHT_28_SEQUENCE=TGCATCGTCAGTGTCATGT
PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG
PRIMER_LEFT_28=9,21
PRIMER_RIGHT_28=30,19
PRIMER_INTERNAL_28=37,22
PRIMER_LEFT_28_TM=57.849
PRIMER_RIGHT_28_TM=57.087
PRIMER_INTERNAL_28_TM=57.841
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.368
PRIMER_INTERNAL_28_GC_PERCENT=54.545
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=0.00
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_LEFT_28_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_28_END_STABILITY=3.9100
PRIMER_RIGHT_28_END_STABILITY=3.2100
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=9.4379
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=3.7339
PRIMER_LEFT_29_PENALTY=2.469564
PRIMER_RIGHT_29_PENALTY=2.989104
PRIMER_INTERNAL_29_PENALTY=4.196497
PRIMER_LEFT_29_SEQUENCE=CATTCGATCCCGACCCAAA
PRIMER_RIGHT_29_SEQUENCE=AGCTATCGATTTGGGTCGG
PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG
PRIMER_LEFT_29=70,19
PRIMER_RIGHT_29=97,19
PRIMER_INTERNAL_29=36,23
PRIMER_LEFT_29_TM=57.530
PRIMER_RIGHT_29_TM=57.011
PRIMER_INTERNAL_29_TM=58.804
PRIMER_LEFT_29_GC_PERCENT=52.632
PRIMER_RIGHT_29_GC_PERCENT=52.632
PRIMER_INTERNAL_29_GC_PERCENT=56.522
PRIMER_INTERNAL_29_SELF_ANY_TH=0.00
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=1.71
PRIMER_RIGHT_29_SELF_ANY_STUCT=Tm: 1.7&deg;C  dG: -5464 cal/mol  dH: -44800 cal/mol  dS: -127 cal/mol*K\n     5' AGCTATCGATTTGGGTCGG 3'\n            ||||||\n3' GGCTGGGTTTAGCTATCGA 5'\n
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=45.50
PRIMER_RIGHT_29_HAIRPIN_TH=41.74
PRIMER_INTERNAL_29_HAIRPIN_TH=0.00
PRIMER_LEFT_29_HAIRPIN_STUCT=Tm: 45.5&deg;C  dG: -600 cal/mol  dH: -22500 cal/mol  dS: -71 cal/mol*K\n   5' CATTCGATU+2510\n         |||  U+2502\n3' AAACCCAGCCCU+2518\n
PRIMER_RIGHT_29_HAIRPIN_STUCT=Tm: 41.7&deg;C  dG: -421 cal/mol  dH: -28000 cal/mol  dS: -89 cal/mol*K\n5' AGCTATCGATTTU+2510\n         |||   U+2502\n     3' GGCTGGGU+2518\n
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence
PRIMER_LEFT_29_END_STABILITY=3.2800
PRIMER_RIGHT_29_END_STABILITY=4.7900
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=2.2809
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_primer_list_and_file_flag_th_4
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33
PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=30
PRIMER_RIGHT_NUM_RETURNED=30
PRIMER_INTERNAL_NUM_RETURNED=30
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.486439
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_INTERNAL_0_PENALTY=0.093814
PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA
PRIMER_LEFT_0=118,20
PRIMER_RIGHT_0=137,20
PRIMER_INTERNAL_0=125,20
PRIMER_LEFT_0_TM=58.514
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=59.906
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_SELF_ANY_TH=7.82
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGA 3'\n     ||   |||  |||   ||\n 3' AGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=36.42
PRIMER_INTERNAL_0_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n          3' AGCCCU+2518\n
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_0_END_STABILITY=5.3400
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_1_PENALTY=1.160593
PRIMER_RIGHT_1_PENALTY=1.160593
PRIMER_INTERNAL_1_PENALTY=0.301069
PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_1=122,19
PRIMER_RIGHT_1=140,19
PRIMER_INTERNAL_1=126,20
PRIMER_LEFT_1_TM=59.161
PRIMER_RIGHT_1_TM=59.161
PRIMER_INTERNAL_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_SELF_ANY_TH=7.82
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n 5' ACGCAAAGCACGCTCC-CGAT 3'\n     ||   |||  |||   ||\n3' TAGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=37.44
PRIMER_INTERNAL_1_HAIRPIN_TH=36.42
PRIMER_RIGHT_1_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n    5' GAGCGTGU+2510\n         ||   C\n3' GTTTTGCGTTTU+2518\n
PRIMER_INTERNAL_1_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n        3' TAGCCCU+2518\n
PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_1_END_STABILITY=5.0300
PRIMER_RIGHT_1_END_STABILITY=2.4400
PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_2_PENALTY=1.656085
PRIMER_RIGHT_2_PENALTY=1.656085
PRIMER_INTERNAL_2_PENALTY=0.900305
PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG
PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA
PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG
PRIMER_LEFT_2=117,21
PRIMER_RIGHT_2=137,21
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=58.344
PRIMER_INTERNAL_2_TM=59.100
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=41.14
PRIMER_INTERNAL_2_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n            3' GCCCU+2518\n
PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=5.3400
PRIMER_RIGHT_2_END_STABILITY=1.7500
PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=8.6247
PRIMER_LEFT_3_PENALTY=1.695359
PRIMER_RIGHT_3_PENALTY=1.695359
PRIMER_INTERNAL_3_PENALTY=0.956520
PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC
PRIMER_LEFT_3=53,21
PRIMER_RIGHT_3=73,21
PRIMER_INTERNAL_3=127,20
PRIMER_LEFT_3_TM=58.305
PRIMER_RIGHT_3_TM=58.305
PRIMER_INTERNAL_3_TM=59.043
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=36.42
PRIMER_INTERNAL_3_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n      3' CTAGCCCU+2518\n
PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=3.7700
PRIMER_RIGHT_3_END_STABILITY=4.0200
PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=13.7493
PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_4_PENALTY=1.831685
PRIMER_RIGHT_4_PENALTY=1.831685
PRIMER_INTERNAL_4_PENALTY=1.018091
PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA
PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT
PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=84,20
PRIMER_INTERNAL_4=125,21
PRIMER_LEFT_4_TM=57.168
PRIMER_RIGHT_4_TM=57.168
PRIMER_INTERNAL_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=57.143
PRIMER_INTERNAL_4_SELF_ANY_TH=7.82
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AACGCAAAGCACGCTCC-CGAT 3'\n     ||   |||  |||   ||\n3' TAGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=35.45
PRIMER_RIGHT_4_HAIRPIN_TH=31.80
PRIMER_INTERNAL_4_HAIRPIN_TH=36.42
PRIMER_LEFT_4_HAIRPIN_STUCT=Tm: 35.4&deg;C  dG: 103 cal/mol  dH: -20400 cal/mol  dS: -66 cal/mol*K\n5' AACGCCAACTCACTU+2510\n            |||  G\n         3' AGTATU+2518\n
PRIMER_RIGHT_4_HAIRPIN_STUCT=Tm: 31.8&deg;C  dG: 377 cal/mol  dH: -22100 cal/mol  dS: -72 cal/mol*K\n         5' TCATAU+2510\n            |||  C\n3' TTGCGGTTGAGTGAU+2518\n
PRIMER_INTERNAL_4_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n         3' TAGCCCU+2518\n
PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=2.1500
PRIMER_RIGHT_4_END_STABILITY=4.8400
PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.8143
PRIMER_LEFT_5_PENALTY=1.902705
PRIMER_RIGHT_5_PENALTY=1.902705
PRIMER_INTERNAL_5_PENALTY=1.178696
PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA
PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_5=62,20
PRIMER_RIGHT_5=81,20
PRIMER_INTERNAL_5=124,21
PRIMER_LEFT_5_TM=57.097
PRIMER_RIGHT_5_TM=57.097
PRIMER_INTERNAL_5_TM=60.179
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_INTERNAL_5_GC_PERCENT=57.143
PRIMER_INTERNAL_5_SELF_ANY_TH=7.82
PRIMER_LEFT_5_SELF_ANY_TH=0.00
PRIMER_RIGHT_5_SELF_ANY_TH=0.00
PRIMER_INTERNAL_5_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGA 3'\n      ||   |||  |||   ||\n  3' AGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_5_SELF_END_TH=0.00
PRIMER_LEFT_5_SELF_END_TH=0.00
PRIMER_RIGHT_5_SELF_END_TH=0.00
PRIMER_LEFT_5_HAIRPIN_TH=0.00
PRIMER_RIGHT_5_HAIRPIN_TH=0.00
PRIMER_INTERNAL_5_HAIRPIN_TH=36.42
PRIMER_INTERNAL_5_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n           3' AGCCCU+2518\n
PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=2.7400
PRIMER_RIGHT_5_END_STABILITY=2.5200
PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=29.7752
PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_6_PENALTY=1.902705
PRIMER_RIGHT_6_PENALTY=1.902705
PRIMER_INTERNAL_6_PENALTY=1.372876
PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT
PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA
PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_6=61,20
PRIMER_RIGHT_6=80,20
PRIMER_INTERNAL_6=126,21
PRIMER_LEFT_6_TM=57.097
PRIMER_RIGHT_6_TM=57.097
PRIMER_INTERNAL_6_TM=60.373
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=61.905
PRIMER_INTERNAL_6_SELF_ANY_TH=7.82
PRIMER_LEFT_6_SELF_ANY_TH=0.00
PRIMER_RIGHT_6_SELF_ANY_TH=0.00
PRIMER_INTERNAL_6_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n  5' ACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_6_SELF_END_TH=0.00
PRIMER_LEFT_6_SELF_END_TH=0.00
PRIMER_RIGHT_6_SELF_END_TH=0.00
PRIMER_LEFT_6_HAIRPIN_TH=0.00
PRIMER_RIGHT_6_HAIRPIN_TH=0.00
PRIMER_INTERNAL_6_HAIRPIN_TH=36.42
PRIMER_INTERNAL_6_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n       3' CTAGCCCU+2518\n
PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=3.5500
PRIMER_RIGHT_6_END_STABILITY=2.1000
PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.0753
PRIMER_LEFT_7_PENALTY=1.950877
PRIMER_RIGHT_7_PENALTY=1.950877
PRIMER_INTERNAL_7_PENALTY=1.393730
PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG
PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA
PRIMER_LEFT_7=119,19
PRIMER_RIGHT_7=137,19
PRIMER_INTERNAL_7=126,19
PRIMER_LEFT_7_TM=58.049
PRIMER_RIGHT_7_TM=58.049
PRIMER_INTERNAL_7_TM=59.606
PRIMER_LEFT_7_GC_PERCENT=47.368
PRIMER_RIGHT_7_GC_PERCENT=47.368
PRIMER_INTERNAL_7_GC_PERCENT=63.158
PRIMER_INTERNAL_7_SELF_ANY_TH=7.82
PRIMER_LEFT_7_SELF_ANY_TH=0.00
PRIMER_RIGHT_7_SELF_ANY_TH=0.00
PRIMER_INTERNAL_7_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' ACGCAAAGCACGCTCC-CGA 3'\n    ||   |||  |||   ||\n3' AGCCC-TCGCACGAAACGCA 5'\n
PRIMER_INTERNAL_7_SELF_END_TH=0.00
PRIMER_LEFT_7_SELF_END_TH=0.00
PRIMER_RIGHT_7_SELF_END_TH=0.00
PRIMER_LEFT_7_HAIRPIN_TH=0.00
PRIMER_RIGHT_7_HAIRPIN_TH=0.00
PRIMER_INTERNAL_7_HAIRPIN_TH=36.42
PRIMER_INTERNAL_7_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n         3' AGCCCU+2518\n
PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=5.3400
PRIMER_RIGHT_7_END_STABILITY=3.5300
PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=14.7637
PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_8_PENALTY=1.972625
PRIMER_RIGHT_8_PENALTY=1.972625
PRIMER_INTERNAL_8_PENALTY=1.595076
PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA
PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_8=56,21
PRIMER_RIGHT_8=76,21
PRIMER_INTERNAL_8=123,21
PRIMER_LEFT_8_TM=58.027
PRIMER_RIGHT_8_TM=58.027
PRIMER_INTERNAL_8_TM=59.405
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_INTERNAL_8_GC_PERCENT=57.143
PRIMER_INTERNAL_8_SELF_ANY_TH=0.00
PRIMER_LEFT_8_SELF_ANY_TH=0.00
PRIMER_RIGHT_8_SELF_ANY_TH=0.00
PRIMER_INTERNAL_8_SELF_END_TH=0.00
PRIMER_LEFT_8_SELF_END_TH=0.00
PRIMER_RIGHT_8_SELF_END_TH=0.00
PRIMER_LEFT_8_HAIRPIN_TH=0.00
PRIMER_RIGHT_8_HAIRPIN_TH=0.00
PRIMER_INTERNAL_8_HAIRPIN_TH=41.14
PRIMER_INTERNAL_8_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n             3' GCCCU+2518\n
PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_LEFT_8_END_STABILITY=3.4100
PRIMER_RIGHT_8_END_STABILITY=3.8600
PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=32.2237
PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.8066
PRIMER_LEFT_9_PENALTY=1.973945
PRIMER_RIGHT_9_PENALTY=1.973945
PRIMER_INTERNAL_9_PENALTY=2.007622
PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC
PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT
PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_9=55,21
PRIMER_RIGHT_9=75,21
PRIMER_INTERNAL_9=121,22
PRIMER_LEFT_9_TM=58.026
PRIMER_RIGHT_9_TM=58.026
PRIMER_INTERNAL_9_TM=59.992
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_INTERNAL_9_GC_PERCENT=59.091
PRIMER_INTERNAL_9_SELF_ANY_TH=0.00
PRIMER_LEFT_9_SELF_ANY_TH=0.00
PRIMER_RIGHT_9_SELF_ANY_TH=0.00
PRIMER_INTERNAL_9_SELF_END_TH=0.00
PRIMER_LEFT_9_SELF_END_TH=0.00
PRIMER_RIGHT_9_SELF_END_TH=0.00
PRIMER_LEFT_9_HAIRPIN_TH=0.00
PRIMER_RIGHT_9_HAIRPIN_TH=0.00
PRIMER_INTERNAL_9_HAIRPIN_TH=0.00
PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=3.0100
PRIMER_RIGHT_9_END_STABILITY=4.0000
PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=27.4392
PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=5.5040
PRIMER_LEFT_10_PENALTY=1.973945
PRIMER_RIGHT_10_PENALTY=1.973945
PRIMER_INTERNAL_10_PENALTY=2.235780
PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT
PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC
PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG
PRIMER_LEFT_10=54,21
PRIMER_RIGHT_10=74,21
PRIMER_INTERNAL_10=125,19
PRIMER_LEFT_10_TM=58.026
PRIMER_RIGHT_10_TM=58.026
PRIMER_INTERNAL_10_TM=58.764
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_INTERNAL_10_GC_PERCENT=63.158
PRIMER_INTERNAL_10_SELF_ANY_TH=0.00
PRIMER_LEFT_10_SELF_ANY_TH=0.00
PRIMER_RIGHT_10_SELF_ANY_TH=0.00
PRIMER_INTERNAL_10_SELF_END_TH=0.00
PRIMER_LEFT_10_SELF_END_TH=0.00
PRIMER_RIGHT_10_SELF_END_TH=0.00
PRIMER_LEFT_10_HAIRPIN_TH=0.00
PRIMER_RIGHT_10_HAIRPIN_TH=0.00
PRIMER_INTERNAL_10_HAIRPIN_TH=41.14
PRIMER_INTERNAL_10_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n           3' GCCCU+2518\n
PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=3.1600
PRIMER_RIGHT_10_END_STABILITY=3.8500
PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=19.5725
PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=9.9844
PRIMER_LEFT_11_PENALTY=2.120321
PRIMER_RIGHT_11_PENALTY=2.120321
PRIMER_INTERNAL_11_PENALTY=2.240074
PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA
PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT
PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_11=64,21
PRIMER_RIGHT_11=84,21
PRIMER_INTERNAL_11=124,22
PRIMER_LEFT_11_TM=57.880
PRIMER_RIGHT_11_TM=57.880
PRIMER_INTERNAL_11_TM=60.240
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_INTERNAL_11_GC_PERCENT=54.545
PRIMER_INTERNAL_11_SELF_ANY_TH=7.82
PRIMER_LEFT_11_SELF_ANY_TH=0.00
PRIMER_RIGHT_11_SELF_ANY_TH=0.00
PRIMER_INTERNAL_11_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGAT 3'\n      ||   |||  |||   ||\n 3' TAGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_11_SELF_END_TH=0.00
PRIMER_LEFT_11_SELF_END_TH=0.00
PRIMER_RIGHT_11_SELF_END_TH=0.00
PRIMER_LEFT_11_HAIRPIN_TH=35.45
PRIMER_RIGHT_11_HAIRPIN_TH=31.80
PRIMER_INTERNAL_11_HAIRPIN_TH=36.42
PRIMER_LEFT_11_HAIRPIN_STUCT=Tm: 35.4&deg;C  dG: 103 cal/mol  dH: -20400 cal/mol  dS: -66 cal/mol*K\n5' AAACGCCAACTCACTU+2510\n             |||  G\n          3' AGTATU+2518\n
PRIMER_RIGHT_11_HAIRPIN_STUCT=Tm: 31.8&deg;C  dG: 377 cal/mol  dH: -22100 cal/mol  dS: -72 cal/mol*K\n          5' TCATAU+2510\n             |||  C\n3' TTTGCGGTTGAGTGAU+2518\n
PRIMER_INTERNAL_11_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n          3' TAGCCCU+2518\n
PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=2.1500
PRIMER_RIGHT_11_END_STABILITY=3.6000
PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=18.7112
PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8905
PRIMER_LEFT_12_PENALTY=2.142778
PRIMER_RIGHT_12_PENALTY=2.142778
PRIMER_INTERNAL_12_PENALTY=2.299276
PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA
PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG
PRIMER_LEFT_12=119,20
PRIMER_RIGHT_12=138,20
PRIMER_INTERNAL_12=122,22
PRIMER_LEFT_12_TM=61.143
PRIMER_RIGHT_12_TM=61.143
PRIMER_INTERNAL_12_TM=60.299
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_INTERNAL_12_GC_PERCENT=59.091
PRIMER_INTERNAL_12_SELF_ANY_TH=0.00
PRIMER_LEFT_12_SELF_ANY_TH=0.00
PRIMER_RIGHT_12_SELF_ANY_TH=0.00
PRIMER_INTERNAL_12_SELF_END_TH=0.00
PRIMER_LEFT_12_SELF_END_TH=0.00
PRIMER_RIGHT_12_SELF_END_TH=0.00
PRIMER_LEFT_12_HAIRPIN_TH=0.00
PRIMER_RIGHT_12_HAIRPIN_TH=0.00
PRIMER_INTERNAL_12_HAIRPIN_TH=41.14
PRIMER_INTERNAL_12_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' CAAAACGCAAAGCACGCTU+2510\n                 ||  U+2502\n              3' GCCCU+2518\n
PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=5.3400
PRIMER_RIGHT_12_END_STABILITY=3.5300
PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=0.2657
PRIMER_LEFT_13_PENALTY=2.299089
PRIMER_RIGHT_13_PENALTY=2.299089
PRIMER_INTERNAL_13_PENALTY=2.427391
PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC
PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_13=123,19
PRIMER_RIGHT_13=141,19
PRIMER_INTERNAL_13=123,22
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.299
PRIMER_INTERNAL_13_TM=60.427
PRIMER_LEFT_13_GC_PERCENT=52.632
PRIMER_RIGHT_13_GC_PERCENT=52.632
PRIMER_INTERNAL_13_GC_PERCENT=54.545
PRIMER_INTERNAL_13_SELF_ANY_TH=7.82
PRIMER_LEFT_13_SELF_ANY_TH=0.00
PRIMER_RIGHT_13_SELF_ANY_TH=0.00
PRIMER_INTERNAL_13_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGA 3'\n       ||   |||  |||   ||\n   3' AGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_13_SELF_END_TH=0.00
PRIMER_LEFT_13_SELF_END_TH=0.00
PRIMER_RIGHT_13_SELF_END_TH=0.00
PRIMER_LEFT_13_HAIRPIN_TH=0.00
PRIMER_RIGHT_13_HAIRPIN_TH=37.44
PRIMER_INTERNAL_13_HAIRPIN_TH=36.42
PRIMER_RIGHT_13_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n  5' GGAGCGTGU+2510\n        ||   C\n3' TTTTGCGTTTU+2518\n
PRIMER_INTERNAL_13_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n            3' AGCCCU+2518\n
PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_13_END_STABILITY=4.7000
PRIMER_RIGHT_13_END_STABILITY=2.4300
PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_14_PENALTY=2.402628
PRIMER_RIGHT_14_PENALTY=2.402628
PRIMER_INTERNAL_14_PENALTY=2.610811
PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT
PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_14=62,21
PRIMER_RIGHT_14=82,21
PRIMER_INTERNAL_14=125,22
PRIMER_LEFT_14_TM=57.597
PRIMER_RIGHT_14_TM=57.597
PRIMER_INTERNAL_14_TM=60.611
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_INTERNAL_14_GC_PERCENT=59.091
PRIMER_INTERNAL_14_SELF_ANY_TH=7.82
PRIMER_LEFT_14_SELF_ANY_TH=0.00
PRIMER_RIGHT_14_SELF_ANY_TH=0.00
PRIMER_INTERNAL_14_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n 5' AACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCAA 5'\n
PRIMER_INTERNAL_14_SELF_END_TH=0.00
PRIMER_LEFT_14_SELF_END_TH=0.00
PRIMER_RIGHT_14_SELF_END_TH=0.00
PRIMER_LEFT_14_HAIRPIN_TH=0.00
PRIMER_RIGHT_14_HAIRPIN_TH=0.00
PRIMER_INTERNAL_14_HAIRPIN_TH=36.42
PRIMER_INTERNAL_14_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AACGCAAAGCACGCTU+2510\n              ||  U+2502\n        3' CTAGCCCU+2518\n
PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=2.2900
PRIMER_RIGHT_14_END_STABILITY=2.5200
PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=30.4432
PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_15_PENALTY=2.402628
PRIMER_RIGHT_15_PENALTY=2.402628
PRIMER_INTERNAL_15_PENALTY=2.740095
PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT
PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT
PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT
PRIMER_LEFT_15=60,21
PRIMER_RIGHT_15=80,21
PRIMER_INTERNAL_15=127,19
PRIMER_LEFT_15_TM=57.597
PRIMER_RIGHT_15_TM=57.597
PRIMER_INTERNAL_15_TM=58.260
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_INTERNAL_15_GC_PERCENT=63.158
PRIMER_INTERNAL_15_SELF_ANY_TH=0.00
PRIMER_LEFT_15_SELF_ANY_TH=0.00
PRIMER_RIGHT_15_SELF_ANY_TH=0.00
PRIMER_INTERNAL_15_SELF_END_TH=0.00
PRIMER_LEFT_15_SELF_END_TH=0.00
PRIMER_RIGHT_15_SELF_END_TH=0.00
PRIMER_LEFT_15_HAIRPIN_TH=0.00
PRIMER_RIGHT_15_HAIRPIN_TH=0.00
PRIMER_INTERNAL_15_HAIRPIN_TH=36.42
PRIMER_INTERNAL_15_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n       3' TAGCCCU+2518\n
PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=3.5500
PRIMER_RIGHT_15_END_STABILITY=1.9800
PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0593
PRIMER_LEFT_16_PENALTY=2.455619
PRIMER_RIGHT_16_PENALTY=2.455619
PRIMER_INTERNAL_16_PENALTY=2.880381
PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG
PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC
PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_16=63,21
PRIMER_RIGHT_16=83,21
PRIMER_INTERNAL_16=120,22
PRIMER_LEFT_16_TM=57.544
PRIMER_RIGHT_16_TM=57.544
PRIMER_INTERNAL_16_TM=59.120
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_INTERNAL_16_GC_PERCENT=54.545
PRIMER_INTERNAL_16_SELF_ANY_TH=0.00
PRIMER_LEFT_16_SELF_ANY_TH=0.00
PRIMER_RIGHT_16_SELF_ANY_TH=0.00
PRIMER_INTERNAL_16_SELF_END_TH=0.00
PRIMER_LEFT_16_SELF_END_TH=0.00
PRIMER_RIGHT_16_SELF_END_TH=0.00
PRIMER_LEFT_16_HAIRPIN_TH=0.00
PRIMER_RIGHT_16_HAIRPIN_TH=0.00
PRIMER_INTERNAL_16_HAIRPIN_TH=0.00
PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner
PRIMER_LEFT_16_END_STABILITY=2.3900
PRIMER_RIGHT_16_END_STABILITY=2.7800
PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=25.0463
PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8601
PRIMER_LEFT_17_PENALTY=2.539776
PRIMER_RIGHT_17_PENALTY=2.539776
PRIMER_INTERNAL_17_PENALTY=2.939429
PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC
PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG
PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_17=122,20
PRIMER_RIGHT_17=141,20
PRIMER_INTERNAL_17=122,21
PRIMER_LEFT_17_TM=61.540
PRIMER_RIGHT_17_TM=61.540
PRIMER_INTERNAL_17_TM=58.061
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_INTERNAL_17_GC_PERCENT=57.143
PRIMER_INTERNAL_17_SELF_ANY_TH=0.00
PRIMER_LEFT_17_SELF_ANY_TH=0.00
PRIMER_RIGHT_17_SELF_ANY_TH=0.00
PRIMER_INTERNAL_17_SELF_END_TH=0.00
PRIMER_LEFT_17_SELF_END_TH=0.00
PRIMER_RIGHT_17_SELF_END_TH=0.00
PRIMER_LEFT_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_TH=37.44
PRIMER_INTERNAL_17_HAIRPIN_TH=0.00
PRIMER_RIGHT_17_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n   5' GGAGCGTGU+2510\n         ||   C\n3' GTTTTGCGTTTU+2518\n
PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_17_END_STABILITY=4.7000
PRIMER_RIGHT_17_END_STABILITY=2.4400
PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=3.7025
PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=8.6631
PRIMER_LEFT_18_PENALTY=2.539776
PRIMER_RIGHT_18_PENALTY=2.539776
PRIMER_INTERNAL_18_PENALTY=2.939429
PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC
PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_18=121,20
PRIMER_RIGHT_18=140,20
PRIMER_INTERNAL_18=121,21
PRIMER_LEFT_18_TM=61.540
PRIMER_RIGHT_18_TM=61.540
PRIMER_INTERNAL_18_TM=58.061
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_INTERNAL_18_GC_PERCENT=57.143
PRIMER_INTERNAL_18_SELF_ANY_TH=0.00
PRIMER_LEFT_18_SELF_ANY_TH=0.00
PRIMER_RIGHT_18_SELF_ANY_TH=0.00
PRIMER_INTERNAL_18_SELF_END_TH=0.00
PRIMER_LEFT_18_SELF_END_TH=0.00
PRIMER_RIGHT_18_SELF_END_TH=0.00
PRIMER_LEFT_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_TH=37.44
PRIMER_INTERNAL_18_HAIRPIN_TH=0.00
PRIMER_RIGHT_18_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n     5' GAGCGTGU+2510\n          ||   C\n3' GGTTTTGCGTTTU+2518\n
PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_18_END_STABILITY=5.0300
PRIMER_RIGHT_18_END_STABILITY=3.2800
PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=9.4619
PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_19_PENALTY=2.587613
PRIMER_RIGHT_19_PENALTY=2.587613
PRIMER_INTERNAL_19_PENALTY=3.476553
PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC
PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT
PRIMER_LEFT_19=120,19
PRIMER_RIGHT_19=138,19
PRIMER_INTERNAL_19=123,23
PRIMER_LEFT_19_TM=60.588
PRIMER_RIGHT_19_TM=60.588
PRIMER_INTERNAL_19_TM=60.477
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=52.632
PRIMER_INTERNAL_19_GC_PERCENT=52.174
PRIMER_INTERNAL_19_SELF_ANY_TH=7.82
PRIMER_LEFT_19_SELF_ANY_TH=0.00
PRIMER_RIGHT_19_SELF_ANY_TH=0.00
PRIMER_INTERNAL_19_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGAT 3'\n       ||   |||  |||   ||\n  3' TAGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_19_SELF_END_TH=0.00
PRIMER_LEFT_19_SELF_END_TH=0.00
PRIMER_RIGHT_19_SELF_END_TH=0.00
PRIMER_LEFT_19_HAIRPIN_TH=0.00
PRIMER_RIGHT_19_HAIRPIN_TH=0.00
PRIMER_INTERNAL_19_HAIRPIN_TH=36.42
PRIMER_INTERNAL_19_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n           3' TAGCCCU+2518\n
PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_19_END_STABILITY=5.3400
PRIMER_RIGHT_19_END_STABILITY=3.5300
PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_20_PENALTY=2.588667
PRIMER_RIGHT_20_PENALTY=2.588667
PRIMER_INTERNAL_20_PENALTY=3.598972
PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT
PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG
PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_20=121,19
PRIMER_RIGHT_20=139,19
PRIMER_INTERNAL_20=119,23
PRIMER_LEFT_20_TM=60.589
PRIMER_RIGHT_20_TM=60.589
PRIMER_INTERNAL_20_TM=59.401
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=52.632
PRIMER_INTERNAL_20_GC_PERCENT=52.174
PRIMER_INTERNAL_20_SELF_ANY_TH=0.00
PRIMER_LEFT_20_SELF_ANY_TH=0.00
PRIMER_RIGHT_20_SELF_ANY_TH=0.00
PRIMER_INTERNAL_20_SELF_END_TH=0.00
PRIMER_LEFT_20_SELF_END_TH=0.00
PRIMER_RIGHT_20_SELF_END_TH=0.00
PRIMER_LEFT_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_TH=37.44
PRIMER_INTERNAL_20_HAIRPIN_TH=0.00
PRIMER_RIGHT_20_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n      5' AGCGTGU+2510\n          ||   C\n3' GGTTTTGCGTTTU+2518\n
PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner
PRIMER_LEFT_20_END_STABILITY=5.0700
PRIMER_RIGHT_20_END_STABILITY=3.2800
PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=8.4951
PRIMER_LEFT_21_PENALTY=2.627395
PRIMER_RIGHT_21_PENALTY=2.627395
PRIMER_INTERNAL_21_PENALTY=3.606233
PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA
PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT
PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG
PRIMER_LEFT_21=52,21
PRIMER_RIGHT_21=72,21
PRIMER_INTERNAL_21=126,18
PRIMER_LEFT_21_TM=57.373
PRIMER_RIGHT_21_TM=57.373
PRIMER_INTERNAL_21_TM=58.394
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_INTERNAL_21_GC_PERCENT=66.667
PRIMER_INTERNAL_21_SELF_ANY_TH=0.00
PRIMER_LEFT_21_SELF_ANY_TH=0.00
PRIMER_RIGHT_21_SELF_ANY_TH=0.00
PRIMER_INTERNAL_21_SELF_END_TH=0.00
PRIMER_LEFT_21_SELF_END_TH=0.00
PRIMER_RIGHT_21_SELF_END_TH=0.00
PRIMER_LEFT_21_HAIRPIN_TH=0.00
PRIMER_RIGHT_21_HAIRPIN_TH=0.00
PRIMER_INTERNAL_21_HAIRPIN_TH=41.14
PRIMER_INTERNAL_21_HAIRPIN_STUCT=Tm: 41.1&deg;C  dG: -274 cal/mol  dH: -20800 cal/mol  dS: -66 cal/mol*K\n5' ACGCAAAGCACGCTU+2510\n             ||  U+2502\n          3' GCCCU+2518\n
PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_21_END_STABILITY=4.5200
PRIMER_RIGHT_21_END_STABILITY=3.0600
PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=3.9584
PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_22_PENALTY=2.678056
PRIMER_RIGHT_22_PENALTY=2.678056
PRIMER_INTERNAL_22_PENALTY=3.829005
PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT
PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA
PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_22=58,21
PRIMER_RIGHT_22=78,21
PRIMER_INTERNAL_22=124,23
PRIMER_LEFT_22_TM=57.322
PRIMER_RIGHT_22_TM=57.322
PRIMER_INTERNAL_22_TM=60.829
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_INTERNAL_22_GC_PERCENT=56.522
PRIMER_INTERNAL_22_SELF_ANY_TH=7.82
PRIMER_LEFT_22_SELF_ANY_TH=0.00
PRIMER_RIGHT_22_SELF_ANY_TH=0.00
PRIMER_INTERNAL_22_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAACGCAAAGCACGCTCC-CGATC 3'\n      ||   |||  |||   ||\n3' CTAGCCC-TCGCACGAAACGCAAA 5'\n
PRIMER_INTERNAL_22_SELF_END_TH=0.00
PRIMER_LEFT_22_SELF_END_TH=0.00
PRIMER_RIGHT_22_SELF_END_TH=0.00
PRIMER_LEFT_22_HAIRPIN_TH=0.00
PRIMER_RIGHT_22_HAIRPIN_TH=0.00
PRIMER_INTERNAL_22_HAIRPIN_TH=36.42
PRIMER_INTERNAL_22_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAACGCAAAGCACGCTU+2510\n               ||  U+2502\n         3' CTAGCCCU+2518\n
PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_22_END_STABILITY=3.4100
PRIMER_RIGHT_22_END_STABILITY=2.1500
PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=25.2513
PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=19.0855
PRIMER_LEFT_23_PENALTY=2.780624
PRIMER_RIGHT_23_PENALTY=2.780624
PRIMER_INTERNAL_23_PENALTY=3.865064
PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT
PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT
PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_23=62,19
PRIMER_RIGHT_23=80,19
PRIMER_INTERNAL_23=120,21
PRIMER_LEFT_23_TM=57.219
PRIMER_RIGHT_23_TM=57.219
PRIMER_INTERNAL_23_TM=57.135
PRIMER_LEFT_23_GC_PERCENT=47.368
PRIMER_RIGHT_23_GC_PERCENT=47.368
PRIMER_INTERNAL_23_GC_PERCENT=52.381
PRIMER_INTERNAL_23_SELF_ANY_TH=0.00
PRIMER_LEFT_23_SELF_ANY_TH=0.00
PRIMER_RIGHT_23_SELF_ANY_TH=0.00
PRIMER_INTERNAL_23_SELF_END_TH=0.00
PRIMER_LEFT_23_SELF_END_TH=0.00
PRIMER_RIGHT_23_SELF_END_TH=0.00
PRIMER_LEFT_23_HAIRPIN_TH=0.00
PRIMER_RIGHT_23_HAIRPIN_TH=0.00
PRIMER_INTERNAL_23_HAIRPIN_TH=0.00
PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_23_END_STABILITY=3.5500
PRIMER_RIGHT_23_END_STABILITY=2.5200
PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=22.0810
PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=18.8930
PRIMER_LEFT_24_PENALTY=2.999071
PRIMER_RIGHT_24_PENALTY=2.999071
PRIMER_INTERNAL_24_PENALTY=3.905453
PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT
PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA
PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA
PRIMER_LEFT_24=120,20
PRIMER_RIGHT_24=139,20
PRIMER_INTERNAL_24=127,18
PRIMER_LEFT_24_TM=61.999
PRIMER_RIGHT_24_TM=61.999
PRIMER_INTERNAL_24_TM=58.095
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_INTERNAL_24_GC_PERCENT=66.667
PRIMER_INTERNAL_24_SELF_ANY_TH=0.00
PRIMER_LEFT_24_SELF_ANY_TH=0.00
PRIMER_RIGHT_24_SELF_ANY_TH=0.00
PRIMER_INTERNAL_24_SELF_END_TH=0.00
PRIMER_LEFT_24_SELF_END_TH=0.00
PRIMER_RIGHT_24_SELF_END_TH=0.00
PRIMER_LEFT_24_HAIRPIN_TH=0.00
PRIMER_RIGHT_24_HAIRPIN_TH=37.44
PRIMER_INTERNAL_24_HAIRPIN_TH=36.42
PRIMER_RIGHT_24_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n       5' AGCGTGU+2510\n           ||   C\n3' AGGTTTTGCGTTTU+2518\n
PRIMER_INTERNAL_24_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CGCAAAGCACGCTU+2510\n            ||  U+2502\n        3' AGCCCU+2518\n
PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon
PRIMER_LEFT_24_END_STABILITY=5.0700
PRIMER_RIGHT_24_END_STABILITY=3.5300
PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=9.5765
PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_LEFT_25_PENALTY=2.999229
PRIMER_RIGHT_25_PENALTY=2.999229
PRIMER_INTERNAL_25_PENALTY=3.954555
PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG
PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC
PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC
PRIMER_LEFT_25=59,21
PRIMER_RIGHT_25=79,21
PRIMER_INTERNAL_25=120,23
PRIMER_LEFT_25_TM=57.001
PRIMER_RIGHT_25_TM=57.001
PRIMER_INTERNAL_25_TM=60.955
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_INTERNAL_25_GC_PERCENT=56.522
PRIMER_INTERNAL_25_SELF_ANY_TH=0.00
PRIMER_LEFT_25_SELF_ANY_TH=0.00
PRIMER_RIGHT_25_SELF_ANY_TH=0.00
PRIMER_INTERNAL_25_SELF_END_TH=0.00
PRIMER_LEFT_25_SELF_END_TH=0.00
PRIMER_RIGHT_25_SELF_END_TH=0.00
PRIMER_LEFT_25_HAIRPIN_TH=0.00
PRIMER_RIGHT_25_HAIRPIN_TH=0.00
PRIMER_INTERNAL_25_HAIRPIN_TH=0.00
PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_25_END_STABILITY=3.6600
PRIMER_RIGHT_25_END_STABILITY=2.0800
PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=30.7683
PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=20.4326
PRIMER_LEFT_26_PENALTY=2.999229
PRIMER_RIGHT_26_PENALTY=2.999229
PRIMER_INTERNAL_26_PENALTY=4.240075
PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC
PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG
PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA
PRIMER_LEFT_26=57,21
PRIMER_RIGHT_26=77,21
PRIMER_INTERNAL_26=122,23
PRIMER_LEFT_26_TM=57.001
PRIMER_RIGHT_26_TM=57.001
PRIMER_INTERNAL_26_TM=61.240
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_INTERNAL_26_GC_PERCENT=56.522
PRIMER_INTERNAL_26_SELF_ANY_TH=7.82
PRIMER_LEFT_26_SELF_ANY_TH=0.00
PRIMER_RIGHT_26_SELF_ANY_TH=0.00
PRIMER_INTERNAL_26_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' CAAAACGCAAAGCACGCTCC-CGA 3'\n        ||   |||  |||   ||\n    3' AGCCC-TCGCACGAAACGCAAAAC 5'\n
PRIMER_INTERNAL_26_SELF_END_TH=0.00
PRIMER_LEFT_26_SELF_END_TH=0.00
PRIMER_RIGHT_26_SELF_END_TH=0.00
PRIMER_LEFT_26_HAIRPIN_TH=0.00
PRIMER_RIGHT_26_HAIRPIN_TH=0.00
PRIMER_INTERNAL_26_HAIRPIN_TH=36.42
PRIMER_INTERNAL_26_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' CAAAACGCAAAGCACGCTU+2510\n                 ||  U+2502\n             3' AGCCCU+2518\n
PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_26_END_STABILITY=3.5100
PRIMER_RIGHT_26_END_STABILITY=2.9000
PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=20.2809
PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.6247
PRIMER_LEFT_27_PENALTY=3.455227
PRIMER_RIGHT_27_PENALTY=3.455227
PRIMER_INTERNAL_27_PENALTY=4.486709
PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC
PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT
PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC
PRIMER_LEFT_27=118,21
PRIMER_RIGHT_27=138,21
PRIMER_INTERNAL_27=119,22
PRIMER_LEFT_27_TM=61.455
PRIMER_RIGHT_27_TM=61.455
PRIMER_INTERNAL_27_TM=57.513
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_INTERNAL_27_GC_PERCENT=50.000
PRIMER_INTERNAL_27_SELF_ANY_TH=0.00
PRIMER_LEFT_27_SELF_ANY_TH=0.00
PRIMER_RIGHT_27_SELF_ANY_TH=0.00
PRIMER_INTERNAL_27_SELF_END_TH=0.00
PRIMER_LEFT_27_SELF_END_TH=0.00
PRIMER_RIGHT_27_SELF_END_TH=0.00
PRIMER_LEFT_27_HAIRPIN_TH=0.00
PRIMER_RIGHT_27_HAIRPIN_TH=0.00
PRIMER_INTERNAL_27_HAIRPIN_TH=0.00
PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner
PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_LEFT_27_END_STABILITY=5.3400
PRIMER_RIGHT_27_END_STABILITY=3.0100
PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=14.0730
PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=1.4431
PRIMER_LEFT_28_PENALTY=3.677573
PRIMER_RIGHT_28_PENALTY=3.677573
PRIMER_INTERNAL_28_PENALTY=4.513970
PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC
PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT
PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC
PRIMER_LEFT_28=123,20
PRIMER_RIGHT_28=142,20
PRIMER_INTERNAL_28=118,24
PRIMER_LEFT_28_TM=62.678
PRIMER_RIGHT_28_TM=62.678
PRIMER_INTERNAL_28_TM=59.486
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_INTERNAL_28_GC_PERCENT=50.000
PRIMER_INTERNAL_28_SELF_ANY_TH=0.00
PRIMER_LEFT_28_SELF_ANY_TH=0.00
PRIMER_RIGHT_28_SELF_ANY_TH=0.00
PRIMER_INTERNAL_28_SELF_END_TH=0.00
PRIMER_LEFT_28_SELF_END_TH=0.00
PRIMER_RIGHT_28_SELF_END_TH=0.00
PRIMER_LEFT_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_TH=37.44
PRIMER_INTERNAL_28_HAIRPIN_TH=0.00
PRIMER_RIGHT_28_HAIRPIN_STUCT=Tm: 37.4&deg;C  dG: -28 cal/mol  dH: -19600 cal/mol  dS: -63 cal/mol*K\n 5' GGGAGCGTGU+2510\n        ||   C\n3' TTTTGCGTTTU+2518\n
PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_28_END_STABILITY=4.3000
PRIMER_RIGHT_28_END_STABILITY=2.4300
PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.1346
PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=10.6160
PRIMER_LEFT_29_PENALTY=4.100862
PRIMER_RIGHT_29_PENALTY=4.100862
PRIMER_INTERNAL_29_PENALTY=5.029817
PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC
PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC
PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC
PRIMER_LEFT_29=128,19
PRIMER_RIGHT_29=146,19
PRIMER_INTERNAL_29=123,24
PRIMER_LEFT_29_TM=62.101
PRIMER_RIGHT_29_TM=62.101
PRIMER_INTERNAL_29_TM=61.030
PRIMER_LEFT_29_GC_PERCENT=63.158
PRIMER_RIGHT_29_GC_PERCENT=63.158
PRIMER_INTERNAL_29_GC_PERCENT=54.167
PRIMER_INTERNAL_29_SELF_ANY_TH=7.82
PRIMER_LEFT_29_SELF_ANY_TH=0.00
PRIMER_RIGHT_29_SELF_ANY_TH=0.00
PRIMER_INTERNAL_29_SELF_ANY_STUCT=Tm: 7.8&deg;C  dG: -1724 cal/mol  dH: -91400 cal/mol  dS: -289 cal/mol*K\n5' AAAACGCAAAGCACGCTCC-CGATC 3'\n       ||   |||  |||   ||\n 3' CTAGCCC-TCGCACGAAACGCAAAA 5'\n
PRIMER_INTERNAL_29_SELF_END_TH=0.00
PRIMER_LEFT_29_SELF_END_TH=0.00
PRIMER_RIGHT_29_SELF_END_TH=0.00
PRIMER_LEFT_29_HAIRPIN_TH=38.29
PRIMER_RIGHT_29_HAIRPIN_TH=0.00
PRIMER_INTERNAL_29_HAIRPIN_TH=36.42
PRIMER_LEFT_29_HAIRPIN_STUCT=Tm: 38.3&deg;C  dG: -93 cal/mol  dH: -22500 cal/mol  dS: -72 cal/mol*K\n5' GCAAAGCACGCTU+2510\n           ||  U+2502\n     3' CTAGCCCU+2518\n
PRIMER_INTERNAL_29_HAIRPIN_STUCT=Tm: 36.4&deg;C  dG: 42 cal/mol  dH: -22500 cal/mol  dS: -73 cal/mol*K\n5' AAAACGCAAAGCACGCTU+2510\n                ||  U+2502\n          3' CTAGCCCU+2518\n
PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_29_END_STABILITY=3.6900
PRIMER_RIGHT_29_END_STABILITY=3.6800
PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000
PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0362
=
PRIMER_TASK=pick_sequencing_primers
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_PICK_RIGHT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_NUM_RETURN=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_sequencing_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
PRIMER_PICK_LEFT_PRIMER=1
SEQUENCE_ID=pick_sequencing_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_WARNING=No left primer found in range 1 - 40
PRIMER_LEFT_EXPLAIN=sequencing locations 1, considered 56, too many Ns 5, low tm 51, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
PRIMER_PICK_RIGHT_PRIMER=1
SEQUENCE_ID=pick_sequencing_primers_th_3
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_WARNING=No left primer found in range 1 - 40
PRIMER_LEFT_EXPLAIN=sequencing locations 1, considered 56, too many Ns 5, low tm 51, ok 0
PRIMER_RIGHT_EXPLAIN=sequencing locations 1, considered 63, low tm 33, high tm 13, ok 1
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.486439
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT
PRIMER_RIGHT_0=137,20
PRIMER_RIGHT_0_TM=58.514
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon
PRIMER_RIGHT_0_END_STABILITY=3.0100
PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=1.4431
=
PRIMER_TASK=check_primers
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=check_primers_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
SEQUENCE_PRIMER=GACATGACACTGACGATGCA
SEQUENCE_PRIMER_REVCOMP=TAGCTATCGATTTGGGTCGG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, low tm 1, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=check_primers_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_PRIMER=TGACCTGATAGAAACGCCAA
SEQUENCE_PRIMER_REVCOMP=TGCTTTGCGTTTTGGAATAG
PRIMER_LEFT_EXPLAIN=considered 1, low tm 1, ok 0
PRIMER_RIGHT_EXPLAIN=considered 1, low tm 1, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_TASK=pick_discriminative_primers
PRIMER_PRODUCT_SIZE_RANGE=75-200
SEQUENCE_ID=pick_disc_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 3, low tm 2, ok 3
PRIMER_RIGHT_EXPLAIN=considered 3, low tm 3, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_PRODUCT_SIZE_RANGE=75-100
SEQUENCE_ID=pick_disc_primer_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_TARGET=37,21
PRIMER_LEFT_EXPLAIN=considered 3, low tm 3, ok 3
PRIMER_RIGHT_EXPLAIN=considered 3, low tm 2, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
PRIMER_TASK=pick_cloning_primers
PRIMER_PRODUCT_SIZE_RANGE=200-300
SEQUENCE_ID=pick_disc_th_1
SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=2,100
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
PRIMER_PRODUCT_SIZE_RANGE=200-300
SEQUENCE_ID=pick_disc_primer_th_2
SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC
SEQUENCE_TARGET=2,120
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
