PRIMER_OPT_SIZE=20
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_MAX_POLY_X=100
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
PRIMER_MAX_GC=80.0
PRIMER_MAX_SIZE=25
PRIMER_MAX_TM=63.0
PRIMER_MIN_SIZE=18
PRIMER_MIN_GC=20
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=6.99
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=6.99
SEQUENCE_ID=14.RHAP64FE2.seq
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
SEQUENCE_INCLUDED_REGION=19,432
SEQUENCE_TARGET=44,46
SEQUENCE_TARGET=92,33
SEQUENCE_TARGET=140,26
SEQUENCE_TARGET=170,14
SEQUENCE_TARGET=211,20
SEQUENCE_TARGET=238,26
SEQUENCE_TARGET=336,14
PRIMER_LEFT_EXPLAIN=considered 443, too many Ns 288, low tm 128, high tm 9, high end compl 12, ok 6
PRIMER_RIGHT_EXPLAIN=considered 599, too many Ns 324, low tm 273, ok 2
PRIMER_PAIR_EXPLAIN=considered 41, unacceptable product size 36, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.458561
PRIMER_LEFT_0_PENALTY=1.104149
PRIMER_RIGHT_0_PENALTY=5.354412
PRIMER_LEFT_0_SEQUENCE=TCCACATGTGCACAGTGGTAT
PRIMER_RIGHT_0_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_0=21,21
PRIMER_RIGHT_0=313,23
PRIMER_LEFT_0_TM=59.896
PRIMER_RIGHT_0_TM=57.646
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=293
PRIMER_PAIR_0_PRODUCT_TM=64.3
PRIMER_PAIR_1_PENALTY=8.467518
PRIMER_LEFT_1_PENALTY=3.113106
PRIMER_RIGHT_1_PENALTY=5.354412
PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT
PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_1=23,21
PRIMER_RIGHT_1=313,23
PRIMER_LEFT_1_TM=57.887
PRIMER_RIGHT_1_TM=57.646
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=291
PRIMER_PAIR_1_PRODUCT_TM=64.2
PRIMER_PAIR_2_PENALTY=8.789928
PRIMER_LEFT_2_PENALTY=1.104149
PRIMER_RIGHT_2_PENALTY=7.685778
PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT
PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT
PRIMER_LEFT_2=21,21
PRIMER_RIGHT_2=288,25
PRIMER_LEFT_2_TM=59.896
PRIMER_RIGHT_2_TM=57.314
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=24.000
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=268
PRIMER_PAIR_2_PRODUCT_TM=63.2
PRIMER_PAIR_3_PENALTY=9.104196
PRIMER_LEFT_3_PENALTY=3.749784
PRIMER_RIGHT_3_PENALTY=5.354412
PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT
PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=313,23
PRIMER_LEFT_3_TM=61.750
PRIMER_RIGHT_3_TM=57.646
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=292
PRIMER_PAIR_3_PRODUCT_TM=64.3
PRIMER_PAIR_4_PENALTY=10.198358
PRIMER_LEFT_4_PENALTY=4.843946
PRIMER_RIGHT_4_PENALTY=5.354412
PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG
PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=313,23
PRIMER_LEFT_4_TM=62.844
PRIMER_RIGHT_4_TM=57.646
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=293
PRIMER_PAIR_4_PRODUCT_TM=64.3
=
