SEQUENCE_ID=negative first base index
[encoded data, 4379 characters]
PRIMER_EXPLAIN_FLAG=1
PRIMER_FIRST_BASE_INDEX=-200
PRIMER_NUM_RETURN=5
SEQUENCE_INCLUDED_REGION=-200,4361
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_MIN_THREE_PRIME_DISTANCE=0
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=25
PRIMER_OPT_GC_PERCENT=50
PRIMER_MIN_GC=45
PRIMER_MAX_GC=55
PRIMER_OPT_TM=60
PRIMER_MIN_TM=55
PRIMER_MAX_TM=80
PRIMER_GC_CLAMP=0
PRIMER_PAIR_MAX_DIFF_TM=40
PRIMER_MAX_POLY_X=5
PRIMER_MAX_SELF_ANY_TH=47
PRIMER_MAX_SELF_END_TH=47
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47
PRIMER_LIBERAL_BASE=0
PRIMER_MAX_NS_ACCEPTED=8
PRIMER_DNA_CONC=50
PRIMER_DNTP_CONC=0.6
PRIMER_SALT_MONOVALENT=50
PRIMER_SALT_DIVALENT=1.5
=
