SEQUENCE_EXCLUDED_REGION=0, 37
SEQUENCE_EXCLUDED_REGION=447, 285
SEQUENCE_EXCLUDED_REGION=961, 290
SEQUENCE_EXCLUDED_REGION=2016, 262
SEQUENCE_EXCLUDED_REGION=2564, 287
SEQUENCE_EXCLUDED_REGION=2880, 99
SEQUENCE_EXCLUDED_REGION=3489, 263
SEQUENCE_EXCLUDED_REGION=3820, 283
SEQUENCE_EXCLUDED_REGION=4495, 277
SEQUENCE_EXCLUDED_REGION=4807, 286
SEQUENCE_EXCLUDED_REGION=5100, 276
SEQUENCE_EXCLUDED_REGION=6209, 288
SEQUENCE_EXCLUDED_REGION=6828, 89
SEQUENCE_EXCLUDED_REGION=6981, 129
SEQUENCE_EXCLUDED_REGION=7127, 205
SEQUENCE_EXCLUDED_REGION=7388, 132
SEQUENCE_EXCLUDED_REGION=8552, 166
SEQUENCE_EXCLUDED_REGION=8799, 277
SEQUENCE_EXCLUDED_REGION=9101, 196
SEQUENCE_EXCLUDED_REGION=9300, 301
SEQUENCE_EXCLUDED_REGION=10228, 283
SEQUENCE_EXCLUDED_REGION=10530, 281
SEQUENCE_EXCLUDED_REGION=11013, 243
SEQUENCE_EXCLUDED_REGION=11304, 281
SEQUENCE_EXCLUDED_REGION=11626, 255
SEQUENCE_EXCLUDED_REGION=11938, 291
SEQUENCE_EXCLUDED_REGION=12254, 284
SEQUENCE_EXCLUDED_REGION=12557, 147
SEQUENCE_EXCLUDED_REGION=13103, 160
SEQUENCE_EXCLUDED_REGION=13266, 288
SEQUENCE_EXCLUDED_REGION=14765, 732
SEQUENCE_EXCLUDED_REGION=15794, 127
SEQUENCE_EXCLUDED_REGION=15997, 85
SEQUENCE_EXCLUDED_REGION=16402, 51
SEQUENCE_EXCLUDED_REGION=16880, 59
SEQUENCE_EXCLUDED_REGION=17065, 124
SEQUENCE_EXCLUDED_REGION=17708, 65
SEQUENCE_EXCLUDED_REGION=17929, 78
SEQUENCE_EXCLUDED_REGION=18125, 124
SEQUENCE_EXCLUDED_REGION=18356, 65
SEQUENCE_EXCLUDED_REGION=18581, 87
SEQUENCE_EXCLUDED_REGION=18692, 183
SEQUENCE_EXCLUDED_REGION=19248, 275
SEQUENCE_EXCLUDED_REGION=20438, 24
SEQUENCE_EXCLUDED_REGION=20722, 99
SEQUENCE_EXCLUDED_REGION=20870, 151
SEQUENCE_EXCLUDED_REGION=21401, 114
SEQUENCE_EXCLUDED_REGION=21928, 284
SEQUENCE_EXCLUDED_REGION=22834, 155
SEQUENCE_EXCLUDED_REGION=22992, 287
SEQUENCE_EXCLUDED_REGION=23282, 138
SEQUENCE_EXCLUDED_REGION=23435, 69
SEQUENCE_EXCLUDED_REGION=23459, 280
SEQUENCE_EXCLUDED_REGION=23753, 288
SEQUENCE_EXCLUDED_REGION=24338, 290
SEQUENCE_EXCLUDED_REGION=24591, 511
SEQUENCE_EXCLUDED_REGION=26124, 287
SEQUENCE_EXCLUDED_REGION=27286, 276
SEQUENCE_EXCLUDED_REGION=27667, 278
SEQUENCE_EXCLUDED_REGION=28313, 91
SEQUENCE_EXCLUDED_REGION=28585, 286
SEQUENCE_EXCLUDED_REGION=28957, 286
SEQUENCE_EXCLUDED_REGION=29255, 287
SEQUENCE_EXCLUDED_REGION=29573, 45
SEQUENCE_EXCLUDED_REGION=29801, 468
SEQUENCE_EXCLUDED_REGION=30461, 180
SEQUENCE_EXCLUDED_REGION=30701, 173
SEQUENCE_EXCLUDED_REGION=31589, 220
SEQUENCE_EXCLUDED_REGION=31992, 287
SEQUENCE_EXCLUDED_REGION=32545, 277
SEQUENCE_EXCLUDED_REGION=32848, 68
SEQUENCE_EXCLUDED_REGION=32956, 89
SEQUENCE_EXCLUDED_REGION=33079, 279
SEQUENCE_EXCLUDED_REGION=33371, 117
SEQUENCE_EXCLUDED_REGION=33758, 284
SEQUENCE_EXCLUDED_REGION=34333, 282
SEQUENCE_EXCLUDED_REGION=35341, 108
SEQUENCE_EXCLUDED_REGION=36347, 247
SEQUENCE_EXCLUDED_REGION=36687, 275
SEQUENCE_EXCLUDED_REGION=37003, 286
SEQUENCE_EXCLUDED_REGION=37918, 217
SEQUENCE_EXCLUDED_REGION=38979, 286
SEQUENCE_EXCLUDED_REGION=39515, 700
SEQUENCE_EXCLUDED_REGION=40312, 93
SEQUENCE_EXCLUDED_REGION=40510, 77
SEQUENCE_EXCLUDED_REGION=40691, 236
SEQUENCE_EXCLUDED_REGION=41066, 187
SEQUENCE_EXCLUDED_REGION=41700, 94
SEQUENCE_EXCLUDED_REGION=42159, 126
SEQUENCE_EXCLUDED_REGION=42462, 159
SEQUENCE_EXCLUDED_REGION=43291, 218
SEQUENCE_EXCLUDED_REGION=44058, 109
SEQUENCE_EXCLUDED_REGION=44262, 38
SEQUENCE_EXCLUDED_REGION=44682, 275
SEQUENCE_EXCLUDED_REGION=45145, 285
SEQUENCE_EXCLUDED_REGION=45464, 277
SEQUENCE_EXCLUDED_REGION=46126, 276
SEQUENCE_EXCLUDED_REGION=46471, 288
SEQUENCE_EXCLUDED_REGION=46767, 118
SEQUENCE_EXCLUDED_REGION=46888, 282
SEQUENCE_EXCLUDED_REGION=47260, 94
SEQUENCE_EXCLUDED_REGION=47372, 94
SEQUENCE_EXCLUDED_REGION=47495, 134
SEQUENCE_EXCLUDED_REGION=47854, 285
SEQUENCE_EXCLUDED_REGION=48185, 122
SEQUENCE_EXCLUDED_REGION=48310, 284
SEQUENCE_EXCLUDED_REGION=48598, 142
SEQUENCE_EXCLUDED_REGION=49033, 286
SEQUENCE_EXCLUDED_REGION=49926, 273
SEQUENCE_EXCLUDED_REGION=50195, 72
SEQUENCE_EXCLUDED_REGION=50218, 286
SEQUENCE_EXCLUDED_REGION=50994, 262
SEQUENCE_EXCLUDED_REGION=51822, 171
SEQUENCE_EXCLUDED_REGION=51999, 280
SEQUENCE_EXCLUDED_REGION=52294, 284
SEQUENCE_EXCLUDED_REGION=52581, 132
SEQUENCE_EXCLUDED_REGION=53675, 287
SEQUENCE_EXCLUDED_REGION=54157, 128
SEQUENCE_EXCLUDED_REGION=54796, 1260
SEQUENCE_EXCLUDED_REGION=54910, 466
SEQUENCE_EXCLUDED_REGION=56696, 288
SEQUENCE_EXCLUDED_REGION=57124, 275
SEQUENCE_EXCLUDED_REGION=57404, 166
SEQUENCE_EXCLUDED_REGION=0,58
SEQUENCE_EXCLUDED_REGION=5975,51652
PRIMER_OPT_TM=65
PRIMER_MIN_TM=60
PRIMER_MAX_TM=70
[encoded data, 57645 characters]
PRIMER_OPT_SIZE=27
PRIMER_MIN_SIZE=25
PRIMER_MAX_SIZE=30
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.021135
PRIMER_LEFT_0_PENALTY=0.016014
PRIMER_RIGHT_0_PENALTY=0.005121
PRIMER_LEFT_0_SEQUENCE=CTCCTCCACTCTCAGTACACATGGTTT
PRIMER_RIGHT_0_SEQUENCE=GTTTACTATGGCACACACATTCCCATT
PRIMER_LEFT_0=3159,27
PRIMER_RIGHT_0=3425,27
PRIMER_LEFT_0_TM=64.984
PRIMER_RIGHT_0_TM=65.005
PRIMER_LEFT_0_GC_PERCENT=48.148
PRIMER_RIGHT_0_GC_PERCENT=40.741
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=267
PRIMER_PAIR_0_PRODUCT_TM=77.8
PRIMER_PAIR_1_PENALTY=0.028467
PRIMER_LEFT_1_PENALTY=0.012719
PRIMER_RIGHT_1_PENALTY=0.015747
PRIMER_LEFT_1_SEQUENCE=CAAGGATTAATAACAAAGGTGGCATCA
PRIMER_RIGHT_1_SEQUENCE=AAACCCATCATGAGACTGCAAAAACTA
PRIMER_LEFT_1=4171,27
PRIMER_RIGHT_1=4344,27
PRIMER_LEFT_1_TM=65.013
PRIMER_RIGHT_1_TM=64.984
PRIMER_LEFT_1_GC_PERCENT=37.037
PRIMER_RIGHT_1_GC_PERCENT=37.037
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=174
PRIMER_PAIR_1_PRODUCT_TM=73.4
PRIMER_PAIR_2_PENALTY=0.031051
PRIMER_LEFT_2_PENALTY=0.016014
PRIMER_RIGHT_2_PENALTY=0.015037
PRIMER_LEFT_2_SEQUENCE=CTCCTCCACTCTCAGTACACATGGTTT
PRIMER_RIGHT_2_SEQUENCE=GACTTAGGATGGCATTCCCAATACTTC
PRIMER_LEFT_2=3159,27
PRIMER_RIGHT_2=3348,27
PRIMER_LEFT_2_TM=64.984
PRIMER_RIGHT_2_TM=64.985
PRIMER_LEFT_2_GC_PERCENT=48.148
PRIMER_RIGHT_2_GC_PERCENT=44.444
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=190
PRIMER_PAIR_2_PRODUCT_TM=78.5
PRIMER_PAIR_3_PENALTY=0.067964
PRIMER_LEFT_3_PENALTY=0.057575
PRIMER_RIGHT_3_PENALTY=0.010389
PRIMER_LEFT_3_SEQUENCE=TCAGTAAAATTCACCTGGAACTAGCCA
PRIMER_RIGHT_3_SEQUENCE=AAGCCACCAAATTCTTCATGTCAGATA
PRIMER_LEFT_3=1752,27
PRIMER_RIGHT_3=1928,27
PRIMER_LEFT_3_TM=65.058
PRIMER_RIGHT_3_TM=64.990
PRIMER_LEFT_3_GC_PERCENT=40.741
PRIMER_RIGHT_3_GC_PERCENT=37.037
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=177
PRIMER_PAIR_3_PRODUCT_TM=75.5
PRIMER_PAIR_4_PENALTY=0.071844
PRIMER_LEFT_4_PENALTY=0.056807
PRIMER_RIGHT_4_PENALTY=0.015037
PRIMER_LEFT_4_SEQUENCE=ATGAGGTACATTGAGTATCCACAGGGA
PRIMER_RIGHT_4_SEQUENCE=GACTTAGGATGGCATTCCCAATACTTC
PRIMER_LEFT_4=3056,27
PRIMER_RIGHT_4=3348,27
PRIMER_LEFT_4_TM=64.943
PRIMER_RIGHT_4_TM=64.985
PRIMER_LEFT_4_GC_PERCENT=44.444
PRIMER_RIGHT_4_GC_PERCENT=44.444
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=293
PRIMER_PAIR_4_PRODUCT_TM=79.1
=
[encoded data, 57645 characters]
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.029383
PRIMER_LEFT_0_PENALTY=0.016035
PRIMER_RIGHT_0_PENALTY=0.013348
PRIMER_LEFT_0_SEQUENCE=CCTGTAATCCCAGCTATTCAGGAGACT
PRIMER_RIGHT_0_SEQUENCE=TCTTGCCTTACTGCACTTTTCTTGTTC
PRIMER_LEFT_0=46922,27
PRIMER_RIGHT_0=47193,27
PRIMER_LEFT_0_TM=65.016
PRIMER_RIGHT_0_TM=64.987
PRIMER_LEFT_0_GC_PERCENT=48.148
PRIMER_RIGHT_0_GC_PERCENT=40.741
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=272
PRIMER_PAIR_0_PRODUCT_TM=75.0
PRIMER_PAIR_1_PENALTY=0.037967
PRIMER_LEFT_1_PENALTY=0.019571
PRIMER_RIGHT_1_PENALTY=0.018396
PRIMER_LEFT_1_SEQUENCE=ATTCCCTTTTGGCTCTCCTTTTCTAAG
PRIMER_RIGHT_1_SEQUENCE=GCTCATTACTGTATGACCATCTTTGCC
PRIMER_LEFT_1=52888,27
PRIMER_RIGHT_1=53112,27
PRIMER_LEFT_1_TM=65.020
PRIMER_RIGHT_1_TM=65.018
PRIMER_LEFT_1_GC_PERCENT=40.741
PRIMER_RIGHT_1_GC_PERCENT=44.444
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=225
PRIMER_PAIR_1_PRODUCT_TM=75.5
PRIMER_PAIR_2_PENALTY=0.045517
PRIMER_LEFT_2_PENALTY=0.029770
PRIMER_RIGHT_2_PENALTY=0.015747
PRIMER_LEFT_2_SEQUENCE=ATTGAGGCAAAGAGACAGTGTTCAAAG
PRIMER_RIGHT_2_SEQUENCE=CTGTTCATGCCTTAACAAATGAGTTCC
PRIMER_LEFT_2=53408,27
PRIMER_RIGHT_2=53537,27
PRIMER_LEFT_2_TM=65.030
PRIMER_RIGHT_2_TM=64.984
PRIMER_LEFT_2_GC_PERCENT=40.741
PRIMER_RIGHT_2_GC_PERCENT=40.741
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=130
PRIMER_PAIR_2_PRODUCT_TM=72.9
PRIMER_PAIR_3_PENALTY=0.045517
PRIMER_LEFT_3_PENALTY=0.029770
PRIMER_RIGHT_3_PENALTY=0.015747
PRIMER_LEFT_3_SEQUENCE=ATTGAGGCAAAGAGACAGTGTTCAAAG
PRIMER_RIGHT_3_SEQUENCE=CCTGTTCATGCCTTAACAAATGAGTTC
PRIMER_LEFT_3=53408,27
PRIMER_RIGHT_3=53538,27
PRIMER_LEFT_3_TM=65.030
PRIMER_RIGHT_3_TM=64.984
PRIMER_LEFT_3_GC_PERCENT=40.741
PRIMER_RIGHT_3_GC_PERCENT=40.741
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=131
PRIMER_PAIR_3_PRODUCT_TM=73.2
PRIMER_PAIR_4_PENALTY=0.056639
PRIMER_LEFT_4_PENALTY=0.043292
PRIMER_RIGHT_4_PENALTY=0.013348
PRIMER_LEFT_4_SEQUENCE=CCCCAAACCATCATCCTTACAAATACT
PRIMER_RIGHT_4_SEQUENCE=TCTTGCCTTACTGCACTTTTCTTGTTC
PRIMER_LEFT_4=47083,27
PRIMER_RIGHT_4=47193,27
PRIMER_LEFT_4_TM=65.043
PRIMER_RIGHT_4_TM=64.987
PRIMER_LEFT_4_GC_PERCENT=40.741
PRIMER_RIGHT_4_GC_PERCENT=40.741
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=111
PRIMER_PAIR_4_PRODUCT_TM=68.9
=
