PRIMER_FIRST_BASE_INDEX=1
SEQUENCE_ID=boundary_test_00
PRIMER_MAX_POLY_X=0
PRIMER_INTERNAL_MAX_POLY_X=0
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PRODUCT_SIZE_RANGE=50-100
PRIMER_MIN_TM=0.0
PRIMER_MAX_TM=100.0
PRIMER_MIN_SIZE=25
PRIMER_MAX_SIZE=25
PRIMER_INTERNAL_MIN_SIZE=25
PRIMER_INTERNAL_MAX_SIZE=25
PRIMER_INTERNAL_OPT_SIZE=25
PRIMER_OPT_SIZE=25
PRIMER_MAX_GC=100
PRIMER_MIN_GC=0
PRIMER_MAX_SELF_END=100
PRIMER_MAX_SELF_ANY=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_INTERNAL_MAX_SELF_ANY=100
PRIMER_INTERNAL_MAX_SELF_END=100 
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_TM=30
PRIMER_INTERNAL_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=11.495378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_01
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PRODUCT_SIZE_RANGE=55-100
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 30, too many Ns 28, ok 2
PRIMER_RIGHT_EXPLAIN=considered 30, too many Ns 29, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=2.731673
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=62.732
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=10.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=66.2
=
SEQUENCE_ID=boundary_test_02
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PRODUCT_SIZE_RANGE=80-80
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=2.731673
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=62.732
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=10.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=66.2
=
SEQUENCE_ID=boundary_test_05
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PRODUCT_SIZE_RANGE=80-80
PRIMER_MIN_SIZE=24
PRIMER_MAX_SIZE=24
PRIMER_OPT_SIZE=24
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=0.330996
PRIMER_LEFT_0_PENALTY=0.243965
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=2.731673
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_LEFT_0=3,24
PRIMER_RIGHT_0=82,24
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=62.732
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=10.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=66.2
=
SEQUENCE_ID=boundary_test_11
PRIMER_MAX_NS_ACCEPTED=1
SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 1, ok 4
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 59, ok 1
PRIMER_PAIR_EXPLAIN=considered 8, unacceptable product size 6, no internal oligo 2, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=boundary_test_11
PRIMER_MAX_NS_ACCEPTED=2
PRIMER_INTERNAL_MAX_NS_ACCEPTED=2
SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 52, ok 8
PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=2
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=0.893850
PRIMER_LEFT_0_PENALTY=0.806819
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=3.221174
PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATT
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
PRIMER_LEFT_0=3,24
PRIMER_RIGHT_0=82,24
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=60.807
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=63.221
PRIMER_LEFT_0_GC_PERCENT=31.818
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=11.50
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=11.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=65.2
PRIMER_PAIR_1_PENALTY=2.546959
PRIMER_LEFT_1_PENALTY=0.806819
PRIMER_RIGHT_1_PENALTY=1.740140
PRIMER_INTERNAL_1_PENALTY=3.221174
PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG
PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA
PRIMER_INTERNAL_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
PRIMER_LEFT_1=4,24
PRIMER_RIGHT_1=83,24
PRIMER_INTERNAL_1=30,25
PRIMER_LEFT_1_TM=60.807
PRIMER_RIGHT_1_TM=61.740
PRIMER_INTERNAL_1_TM=63.221
PRIMER_LEFT_1_GC_PERCENT=31.818
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_INTERNAL_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=11.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=11.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_1_PRODUCT_TM=64.7
=
SEQUENCE_ID=boundary_test_12
PRIMER_MAX_NS_ACCEPTED=2
SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_RIGHT_EXPLAIN=considered 5, ok 5
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 52, ok 8
PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=2
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=0.893850
PRIMER_LEFT_0_PENALTY=0.806819
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=3.221174
PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATT
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
PRIMER_LEFT_0=3,24
PRIMER_RIGHT_0=82,24
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=60.807
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=63.221
PRIMER_LEFT_0_GC_PERCENT=31.818
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=11.50
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=11.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=65.2
PRIMER_PAIR_1_PENALTY=2.546959
PRIMER_LEFT_1_PENALTY=0.806819
PRIMER_RIGHT_1_PENALTY=1.740140
PRIMER_INTERNAL_1_PENALTY=3.221174
PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG
PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA
PRIMER_INTERNAL_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG
PRIMER_LEFT_1=4,24
PRIMER_RIGHT_1=83,24
PRIMER_INTERNAL_1=30,25
PRIMER_LEFT_1_TM=60.807
PRIMER_RIGHT_1_TM=61.740
PRIMER_INTERNAL_1_TM=63.221
PRIMER_LEFT_1_GC_PERCENT=31.818
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_INTERNAL_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=11.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=11.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_1_PRODUCT_TM=64.7
=
SEQUENCE_ID=boundary_test_13
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=0
SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 23, ok 37
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=5.394230
PRIMER_LEFT_0_PENALTY=5.307199
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=3.800256
PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN
PRIMER_LEFT_0=3,24
PRIMER_RIGHT_0=82,24
PRIMER_INTERNAL_0=32,25
PRIMER_LEFT_0_TM=54.693
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=56.200
PRIMER_LEFT_0_GC_PERCENT=0.000
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=0.000
PRIMER_LEFT_0_SELF_ANY=0.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=57.0
=
SEQUENCE_ID=boundary_test_14
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=.0001
SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 25, ok 35
PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=4.843553
PRIMER_LEFT_0_PENALTY=4.756523
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=3.800256
PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAACAAAAAA
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN
PRIMER_LEFT_0=3,24
PRIMER_RIGHT_0=82,24
PRIMER_INTERNAL_0=30,25
PRIMER_LEFT_0_TM=55.243
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=56.200
PRIMER_LEFT_0_GC_PERCENT=4.167
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=0.000
PRIMER_LEFT_0_SELF_ANY=0.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=57.5
=
SEQUENCE_ID=boundary_test_15
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=20
PRIMER_MAX_GC=100
SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 25, ok 35
PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=29.395694
PRIMER_LEFT_0_PENALTY=29.308664
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=4.161950
PRIMER_LEFT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTTT
PRIMER_LEFT_0=3,24
PRIMER_RIGHT_0=82,24
PRIMER_INTERNAL_0=33,25
PRIMER_LEFT_0_TM=89.309
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=55.838
PRIMER_LEFT_0_GC_PERCENT=100.000
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=0.000
PRIMER_LEFT_0_SELF_ANY=0.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=1.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=69.8
=
SEQUENCE_ID=boundary_test_16
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_PRODUCT_SIZE_RANGE=50-200
PRIMER_INTERNAL_MAX_NS_ACCEPTED=5
SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 8, ok 2
PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 8, ok 2
PRIMER_INTERNAL_EXPLAIN=considered 35, ok 35
PRIMER_PAIR_EXPLAIN=considered 4, no internal oligo 4, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=boundary_test_17
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=20
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 31, ok 4
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 31, ok 4
PRIMER_INTERNAL_EXPLAIN=considered 60, GC content failed 29, ok 31
PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 4, no internal oligo 12, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=boundary_test_18
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
SEQUENCE_INTERNAL_EXCLUDED_REGION=11,50
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9
PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9
PRIMER_INTERNAL_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8
PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=boundary_test_19
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
PRIMER_INTERNAL_MAX_NS_ACCEPTED=0
SEQUENCE_INTERNAL_EXCLUDED_REGION=11,50
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 66, too many Ns 57, ok 9
PRIMER_RIGHT_EXPLAIN=considered 66, too many Ns 57, ok 9
PRIMER_INTERNAL_EXPLAIN=considered 91, too many Ns 84, overlap excluded region 5, ok 2
PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 3, no internal oligo 8, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.330996
PRIMER_LEFT_0_PENALTY=0.243965
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=5.578209
PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
PRIMER_LEFT_0=4,24
PRIMER_RIGHT_0=113,24
PRIMER_INTERNAL_0=61,25
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=65.578
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=52.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=110
PRIMER_PAIR_0_PRODUCT_TM=71.2
PRIMER_PAIR_1_PENALTY=0.330996
PRIMER_LEFT_1_PENALTY=0.243965
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_INTERNAL_1_PENALTY=5.578209
PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_1_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
PRIMER_LEFT_1=3,24
PRIMER_RIGHT_1=113,24
PRIMER_INTERNAL_1=61,25
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=59.913
PRIMER_INTERNAL_1_TM=65.578
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=37.500
PRIMER_INTERNAL_1_GC_PERCENT=52.000
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=10.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=111
PRIMER_PAIR_1_PRODUCT_TM=71.5
PRIMER_PAIR_2_PENALTY=1.261475
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=1.017510
PRIMER_INTERNAL_2_PENALTY=5.578209
PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_2_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
PRIMER_LEFT_2=4,24
PRIMER_RIGHT_2=112,24
PRIMER_INTERNAL_2=61,25
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=58.982
PRIMER_INTERNAL_2_TM=65.578
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=33.333
PRIMER_INTERNAL_2_GC_PERCENT=52.000
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=10.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=109
PRIMER_PAIR_2_PRODUCT_TM=70.9
PRIMER_PAIR_3_PENALTY=1.261475
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=1.017510
PRIMER_INTERNAL_3_PENALTY=5.578209
PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_3_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
PRIMER_LEFT_3=3,24
PRIMER_RIGHT_3=112,24
PRIMER_INTERNAL_3=61,25
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=58.982
PRIMER_INTERNAL_3_TM=65.578
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=52.000
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=10.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=110
PRIMER_PAIR_3_PRODUCT_TM=71.2
PRIMER_PAIR_4_PENALTY=8.005859
PRIMER_LEFT_4_PENALTY=7.918829
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_INTERNAL_4_PENALTY=5.578209
PRIMER_LEFT_4_SEQUENCE=AAGCTAAGCTTGCCCGTATCCCCT
PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_4_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT
PRIMER_LEFT_4=30,24
PRIMER_RIGHT_4=113,24
PRIMER_INTERNAL_4=61,25
PRIMER_LEFT_4_TM=67.919
PRIMER_RIGHT_4_TM=59.913
PRIMER_INTERNAL_4_TM=65.578
PRIMER_LEFT_4_GC_PERCENT=54.167
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_INTERNAL_4_GC_PERCENT=52.000
PRIMER_LEFT_4_SELF_ANY=9.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
PRIMER_PAIR_4_PRODUCT_TM=70.8
=
SEQUENCE_ID=boundary_test_20
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
PRIMER_PRODUCT_SIZE_RANGE=100-300
SEQUENCE_INTERNAL_EXCLUDED_REGION=6,50
SEQUENCE_INTERNAL_EXCLUDED_REGION=61,15
SEQUENCE_INCLUDED_REGION=3,250
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT
PRIMER_LEFT_EXPLAIN=considered 151, too many Ns 99, ok 52
PRIMER_RIGHT_EXPLAIN=considered 151, too many Ns 148, ok 3
PRIMER_INTERNAL_EXPLAIN=considered 226, too many Ns 175, overlap excluded region 47, ok 4
PRIMER_PAIR_EXPLAIN=considered 14, no internal oligo 8, ok 6
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.330996
PRIMER_LEFT_0_PENALTY=0.243965
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=3.811648
PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
PRIMER_LEFT_0=4,24
PRIMER_RIGHT_0=252,24
PRIMER_INTERNAL_0=76,25
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=63.812
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=52.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=12.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=249
PRIMER_PAIR_0_PRODUCT_TM=66.4
PRIMER_PAIR_1_PENALTY=0.330996
PRIMER_LEFT_1_PENALTY=0.243965
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_INTERNAL_1_PENALTY=3.811648
PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_1_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
PRIMER_LEFT_1=3,24
PRIMER_RIGHT_1=252,24
PRIMER_INTERNAL_1=76,25
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=59.913
PRIMER_INTERNAL_1_TM=63.812
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=37.500
PRIMER_INTERNAL_1_GC_PERCENT=52.000
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=10.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=12.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=250
PRIMER_PAIR_1_PRODUCT_TM=66.5
PRIMER_PAIR_2_PENALTY=0.386435
PRIMER_LEFT_2_PENALTY=0.299404
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_INTERNAL_2_PENALTY=3.811648
PRIMER_LEFT_2_SEQUENCE=GGAGACGATACGGTAAGCTAGCTA
PRIMER_RIGHT_2_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_2_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
PRIMER_LEFT_2=51,24
PRIMER_RIGHT_2=252,24
PRIMER_INTERNAL_2=76,25
PRIMER_LEFT_2_TM=60.299
PRIMER_RIGHT_2_TM=59.913
PRIMER_INTERNAL_2_TM=63.812
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=37.500
PRIMER_INTERNAL_2_GC_PERCENT=52.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=12.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=12.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=202
PRIMER_PAIR_2_PRODUCT_TM=64.0
PRIMER_PAIR_3_PENALTY=1.261475
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=1.017510
PRIMER_INTERNAL_3_PENALTY=3.811648
PRIMER_LEFT_3_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_3_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
PRIMER_LEFT_3=4,24
PRIMER_RIGHT_3=251,24
PRIMER_INTERNAL_3=76,25
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=58.982
PRIMER_INTERNAL_3_TM=63.812
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=52.000
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=12.00
PRIMER_LEFT_3_SELF_END=10.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=12.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=248
PRIMER_PAIR_3_PRODUCT_TM=66.2
PRIMER_PAIR_4_PENALTY=1.261475
PRIMER_LEFT_4_PENALTY=0.243965
PRIMER_RIGHT_4_PENALTY=1.017510
PRIMER_INTERNAL_4_PENALTY=3.811648
PRIMER_LEFT_4_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_4_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_4_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG
PRIMER_LEFT_4=3,24
PRIMER_RIGHT_4=251,24
PRIMER_INTERNAL_4=76,25
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=58.982
PRIMER_INTERNAL_4_TM=63.812
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=33.333
PRIMER_INTERNAL_4_GC_PERCENT=52.000
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=12.00
PRIMER_LEFT_4_SELF_END=10.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=12.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=249
PRIMER_PAIR_4_PRODUCT_TM=66.4
=
SEQUENCE_ID=boundary_test_21
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=0
PRIMER_INTERNAL_MIN_TM=55.7
PRIMER_INTERNAL_MAX_TM=55.9
PRIMER_INTERNAL_OPT_TM=55.8
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_EXPLAIN=considered 77, too many Ns 27, ok 50
PRIMER_RIGHT_EXPLAIN=considered 77, too many Ns 28, ok 49
PRIMER_INTERNAL_EXPLAIN=considered 152, too many Ns 57, low tm 1, high tm 93, ok 1
PRIMER_PAIR_EXPLAIN=considered 323, unacceptable product size 223, no internal oligo 87, ok 13
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.330996
PRIMER_LEFT_0_PENALTY=0.243965
PRIMER_RIGHT_0_PENALTY=0.087031
PRIMER_INTERNAL_0_PENALTY=0.038050
PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_LEFT_0=4,24
PRIMER_RIGHT_0=174,24
PRIMER_INTERNAL_0=62,25
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=59.913
PRIMER_INTERNAL_0_TM=55.838
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=0.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=0.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=82.5
PRIMER_PAIR_1_PENALTY=0.330996
PRIMER_LEFT_1_PENALTY=0.243965
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_INTERNAL_1_PENALTY=0.038050
PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_1_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_LEFT_1=3,24
PRIMER_RIGHT_1=174,24
PRIMER_INTERNAL_1=62,25
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=59.913
PRIMER_INTERNAL_1_TM=55.838
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=37.500
PRIMER_INTERNAL_1_GC_PERCENT=0.000
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=10.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=172
PRIMER_PAIR_1_PRODUCT_TM=82.6
PRIMER_PAIR_2_PENALTY=1.261475
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=1.017510
PRIMER_INTERNAL_2_PENALTY=0.038050
PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_2_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_LEFT_2=4,24
PRIMER_RIGHT_2=173,24
PRIMER_INTERNAL_2=62,25
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=58.982
PRIMER_INTERNAL_2_TM=55.838
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=33.333
PRIMER_INTERNAL_2_GC_PERCENT=0.000
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=10.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=170
PRIMER_PAIR_2_PRODUCT_TM=82.4
PRIMER_PAIR_3_PENALTY=1.261475
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=1.017510
PRIMER_INTERNAL_3_PENALTY=0.038050
PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT
PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_3_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_LEFT_3=3,24
PRIMER_RIGHT_3=173,24
PRIMER_INTERNAL_3=62,25
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=58.982
PRIMER_INTERNAL_3_TM=55.838
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=0.000
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=10.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=171
PRIMER_PAIR_3_PRODUCT_TM=82.5
PRIMER_PAIR_4_PENALTY=29.395694
PRIMER_LEFT_4_PENALTY=29.308664
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_INTERNAL_4_PENALTY=0.038050
PRIMER_LEFT_4_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG
PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG
PRIMER_INTERNAL_4_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_LEFT_4=38,24
PRIMER_RIGHT_4=174,24
PRIMER_INTERNAL_4=62,25
PRIMER_LEFT_4_TM=89.309
PRIMER_RIGHT_4_TM=59.913
PRIMER_INTERNAL_4_TM=55.838
PRIMER_LEFT_4_GC_PERCENT=100.000
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_INTERNAL_4_GC_PERCENT=0.000
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=1.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=137
PRIMER_PAIR_4_PRODUCT_TM=83.4
=
SEQUENCE_ID=boundary_test_22
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_PRODUCT_SIZE_RANGE=80-80
PRIMER_MIN_TM=0.0
PRIMER_MAX_TM=100.0
PRIMER_MIN_SIZE=25
PRIMER_MAX_SIZE=25
PRIMER_INTERNAL_MIN_SIZE=25
PRIMER_INTERNAL_MAX_SIZE=25
PRIMER_INTERNAL_OPT_SIZE=25
PRIMER_OPT_SIZE=25
PRIMER_MAX_GC=100
PRIMER_MIN_GC=0
PRIMER_MAX_SELF_END=100
PRIMER_MAX_SELF_ANY=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_INTERNAL_MAX_SELF_ANY=100
PRIMER_INTERNAL_MAX_SELF_END=100 
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_TM=30
PRIMER_INTERNAL_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=0
PRIMER_PICK_INTERNAL_OLIGO=1
P3_COMMENT=should succeed
PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1
PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_23
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=2,1
P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_24
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=3,1
P3_COMMENT=should succeed
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_25
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=1,3
P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region)
PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_26
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=30,1
P3_COMMENT=should succeed
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_27
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=31,1
P3_COMMENT=should fail w/ no legal internal oligo
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=boundary_test_28
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=55,1
P3_COMMENT=should fail w/ no legal internal oligo
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=boundary_test_29
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=56,1
P3_COMMENT=should succeed
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_30
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1
P3_COMMENT=should succeed
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_31
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=83,1
P3_COMMENT=should generate warning (external oligo excluded region outside of included region)
PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_32
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=82,2
P3_COMMENT=should generate warning (external oligo excluded region outside of included region)
PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_33
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
P3_COMMENT=should succeed (testing number of internal oligo excluded regions)
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.381274
PRIMER_LEFT_0_PENALTY=2.731673
PRIMER_RIGHT_0_PENALTY=0.649600
PRIMER_INTERNAL_0_PENALTY=15.695378
PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT_0=3,25
PRIMER_RIGHT_0=82,25
PRIMER_INTERNAL_0=31,25
PRIMER_LEFT_0_TM=62.732
PRIMER_RIGHT_0_TM=60.650
PRIMER_INTERNAL_0_TM=71.495
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=36.000
PRIMER_INTERNAL_0_GC_PERCENT=56.000
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=10.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_0_PRODUCT_TM=68.8
=
SEQUENCE_ID=boundary_test_34
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INCLUDED_REGION=3,80
SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1
P3_COMMENT=should fail (testing number of internal oligo excluded regions)
PRIMER_ERROR=Too many elements for tag SEQUENCE_INTERNAL_EXCLUDED_REGION
=
