SEQUENCE_ID=ok_regions_test1
SEQUENCE_TEMPLATE=GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCAAAAAAAAAAAAAAAA
PRIMER_NUM_RETURN=200
PRIMER_PRODUCT_SIZE_RANGE=200-1000
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_THREE_PRIME_DISTANCE=0
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,216,,;,,238,216
SEQUENCE_OVERLAP_JUNCTION_LIST=256 424 554 668 861 1064 1232 1289 1423 1498 1657 1786 1886 2108 2162 2263 2410
PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=4
PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=7
PRIMER_LEFT_EXPLAIN=considered 34490, GC content failed 759, low tm 295, high tm 2741, high any compl 17, high end compl 93, not in any ok left region 30165, ok 420
PRIMER_RIGHT_EXPLAIN=considered 34490, GC content failed 15, low tm 2036, high tm 6898, high any compl 4, high end compl 47, not in any ok right region 22715, ok 2775
PRIMER_PAIR_EXPLAIN=considered 839536, unacceptable product size 21069, high end compl 38, no overlap of required point 818172, primer in pair overlaps a primer in a better pair 264217, ok 257
PRIMER_LEFT_NUM_RETURNED=200
PRIMER_RIGHT_NUM_RETURNED=200
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=200
PRIMER_PAIR_0_PENALTY=0.120586
PRIMER_LEFT_0_PENALTY=0.112124
PRIMER_RIGHT_0_PENALTY=0.008461
PRIMER_LEFT_0_SEQUENCE=CAGATTGCCATGTTCTGTGG
PRIMER_RIGHT_0_SEQUENCE=TCACTGCATGTCTCTTTGGC
PRIMER_LEFT_0=296,20
PRIMER_RIGHT_0=678,20
PRIMER_LEFT_0_TM=60.112
PRIMER_RIGHT_0_TM=59.992
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=383
PRIMER_PAIR_0_PRODUCT_TM=78.9
PRIMER_PAIR_1_PENALTY=0.290503
PRIMER_LEFT_1_PENALTY=0.112124
PRIMER_RIGHT_1_PENALTY=0.178378
PRIMER_LEFT_1_SEQUENCE=CCAGATTGCCATGTTCTGTG
PRIMER_RIGHT_1_SEQUENCE=TGTTCAGAGCACACCTCTCG
PRIMER_LEFT_1=295,20
PRIMER_RIGHT_1=1080,20
PRIMER_LEFT_1_TM=60.112
PRIMER_RIGHT_1_TM=60.178
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=786
PRIMER_PAIR_1_PRODUCT_TM=80.3
PRIMER_PAIR_2_PENALTY=0.502773
PRIMER_LEFT_2_PENALTY=0.137602
PRIMER_RIGHT_2_PENALTY=0.365170
PRIMER_LEFT_2_SEQUENCE=GGAAGTGGGATTCAGATCCA
PRIMER_RIGHT_2_SEQUENCE=GTAAACTTTGGGACATGGCG
PRIMER_LEFT_2=348,20
PRIMER_RIGHT_2=1247,20
PRIMER_LEFT_2_TM=59.862
PRIMER_RIGHT_2_TM=60.365
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=11.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=900
PRIMER_PAIR_2_PRODUCT_TM=80.9
PRIMER_PAIR_3_PENALTY=0.554615
PRIMER_LEFT_3_PENALTY=0.532607
PRIMER_RIGHT_3_PENALTY=0.022009
PRIMER_LEFT_3_SEQUENCE=GCTGGAGGTACCCACTGATG
PRIMER_RIGHT_3_SEQUENCE=TGCTGGTGGTTCTCTCTGTG
PRIMER_LEFT_3=250,20
PRIMER_RIGHT_3=1013,20
PRIMER_LEFT_3_TM=60.533
PRIMER_RIGHT_3_TM=60.022
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=764
PRIMER_PAIR_3_PRODUCT_TM=80.3
PRIMER_PAIR_4_PENALTY=0.673790
PRIMER_LEFT_4_PENALTY=0.220845
PRIMER_RIGHT_4_PENALTY=0.452945
PRIMER_LEFT_4_SEQUENCE=ATGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_4_SEQUENCE=CTCACCAACTAAGCAGCGGT
PRIMER_LEFT_4=336,20
PRIMER_RIGHT_4=562,20
PRIMER_LEFT_4_TM=59.779
PRIMER_RIGHT_4_TM=60.453
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=227
PRIMER_PAIR_4_PRODUCT_TM=78.6
PRIMER_PAIR_5_PENALTY=0.690561
PRIMER_LEFT_5_PENALTY=0.237616
PRIMER_RIGHT_5_PENALTY=0.452945
PRIMER_LEFT_5_SEQUENCE=TGGAGGTACCCACTGATGGT
PRIMER_RIGHT_5_SEQUENCE=ACTCACCAACTAAGCAGCGG
PRIMER_LEFT_5=252,20
PRIMER_RIGHT_5=563,20
PRIMER_LEFT_5_TM=60.238
PRIMER_RIGHT_5_TM=60.453
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_END_STABILITY=7.8000
PRIMER_RIGHT_5_END_STABILITY=11.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=312
PRIMER_PAIR_5_PRODUCT_TM=79.4
PRIMER_PAIR_6_PENALTY=0.840127
PRIMER_LEFT_6_PENALTY=0.327840
PRIMER_RIGHT_6_PENALTY=0.512286
PRIMER_LEFT_6_SEQUENCE=GTGGCAGACTGAACATGCAC
PRIMER_RIGHT_6_SEQUENCE=TCACCAACTAAGCAGCGGTA
PRIMER_LEFT_6=312,20
PRIMER_RIGHT_6=561,20
PRIMER_LEFT_6_TM=60.328
PRIMER_RIGHT_6_TM=59.488
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=250
PRIMER_PAIR_6_PRODUCT_TM=78.7
PRIMER_PAIR_7_PENALTY=0.916059
PRIMER_LEFT_7_PENALTY=0.203367
PRIMER_RIGHT_7_PENALTY=0.712692
PRIMER_LEFT_7_SEQUENCE=GGACCAAAACCTGCATTGAT
PRIMER_RIGHT_7_SEQUENCE=CACACCTCTCGAACCACCTC
PRIMER_LEFT_7=372,20
PRIMER_RIGHT_7=1071,20
PRIMER_LEFT_7_TM=59.797
PRIMER_RIGHT_7_TM=60.713
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=60.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=700
PRIMER_PAIR_7_PRODUCT_TM=80.4
PRIMER_PAIR_8_PENALTY=1.179338
PRIMER_LEFT_8_PENALTY=1.139192
PRIMER_RIGHT_8_PENALTY=0.040146
PRIMER_LEFT_8_SEQUENCE=TGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_8_SEQUENCE=ACATCCGCCGTAAAAGAATG
PRIMER_LEFT_8=416,21
PRIMER_RIGHT_8=1171,20
PRIMER_LEFT_8_TM=59.861
PRIMER_RIGHT_8_TM=59.960
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=756
PRIMER_PAIR_8_PRODUCT_TM=80.8
PRIMER_PAIR_9_PENALTY=1.179338
PRIMER_LEFT_9_PENALTY=1.139192
PRIMER_RIGHT_9_PENALTY=0.040146
PRIMER_LEFT_9_SEQUENCE=TTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_9_SEQUENCE=CACATCCGCCGTAAAAGAAT
PRIMER_LEFT_9=415,21
PRIMER_RIGHT_9=1172,20
PRIMER_LEFT_9_TM=59.861
PRIMER_RIGHT_9_TM=59.960
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=758
PRIMER_PAIR_9_PRODUCT_TM=80.8
PRIMER_PAIR_10_PENALTY=1.248189
PRIMER_LEFT_10_PENALTY=0.376749
PRIMER_RIGHT_10_PENALTY=0.871441
PRIMER_LEFT_10_SEQUENCE=CTGGAGGTACCCACTGATGG
PRIMER_RIGHT_10_SEQUENCE=TCAGAGCACACCTCTCGAAC
PRIMER_LEFT_10=251,20
PRIMER_RIGHT_10=1077,20
PRIMER_LEFT_10_TM=60.377
PRIMER_RIGHT_10_TM=59.129
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=827
PRIMER_PAIR_10_PRODUCT_TM=80.5
PRIMER_PAIR_11_PENALTY=1.293286
PRIMER_LEFT_11_PENALTY=0.420643
PRIMER_RIGHT_11_PENALTY=0.872644
PRIMER_LEFT_11_SEQUENCE=ACTGATGGTAATGCTGGCCT
PRIMER_RIGHT_11_SEQUENCE=GAGCACACCTCTCGAACCAC
PRIMER_LEFT_11=263,20
PRIMER_RIGHT_11=1074,20
PRIMER_LEFT_11_TM=59.579
PRIMER_RIGHT_11_TM=60.873
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=60.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=812
PRIMER_PAIR_11_PRODUCT_TM=80.5
PRIMER_PAIR_12_PENALTY=1.467010
PRIMER_LEFT_12_PENALTY=0.466916
PRIMER_RIGHT_12_PENALTY=1.000094
PRIMER_LEFT_12_SEQUENCE=GCATTGATACCAAGGAAGGC
PRIMER_RIGHT_12_SEQUENCE=ACTGCATGTCTCTTTGGCG
PRIMER_LEFT_12=384,20
PRIMER_RIGHT_12=676,19
PRIMER_LEFT_12_TM=59.533
PRIMER_RIGHT_12_TM=60.000
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=52.632
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=11.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=293
PRIMER_PAIR_12_PRODUCT_TM=78.7
PRIMER_PAIR_13_PENALTY=1.482050
PRIMER_LEFT_13_PENALTY=0.473276
PRIMER_RIGHT_13_PENALTY=1.008773
PRIMER_LEFT_13_SEQUENCE=CTGTGGCAGACTGAACATGC
PRIMER_RIGHT_13_SEQUENCE=AGAGCACACCTCTCGAACCA
PRIMER_LEFT_13=310,20
PRIMER_RIGHT_13=1075,20
PRIMER_LEFT_13_TM=60.473
PRIMER_RIGHT_13_TM=61.009
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=766
PRIMER_PAIR_13_PRODUCT_TM=80.4
PRIMER_PAIR_14_PENALTY=1.606490
PRIMER_LEFT_14_PENALTY=1.558232
PRIMER_RIGHT_14_PENALTY=0.048259
PRIMER_LEFT_14_SEQUENCE=TGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_14_SEQUENCE=TTCTCATCCCCAGGTGTCTC
PRIMER_LEFT_14=416,20
PRIMER_RIGHT_14=1356,20
PRIMER_LEFT_14_TM=58.442
PRIMER_RIGHT_14_TM=60.048
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=941
PRIMER_PAIR_14_PRODUCT_TM=81.0
PRIMER_PAIR_15_PENALTY=1.672321
PRIMER_LEFT_15_PENALTY=0.663548
PRIMER_RIGHT_15_PENALTY=1.008773
PRIMER_LEFT_15_SEQUENCE=AGATCCATCAGGGACCAAAA
PRIMER_RIGHT_15_SEQUENCE=CAGAGCACACCTCTCGAACC
PRIMER_LEFT_15=361,20
PRIMER_RIGHT_15=1076,20
PRIMER_LEFT_15_TM=59.336
PRIMER_RIGHT_15_TM=61.009
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=60.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=716
PRIMER_PAIR_15_PRODUCT_TM=80.4
PRIMER_PAIR_16_PENALTY=1.692682
PRIMER_LEFT_16_PENALTY=0.663548
PRIMER_RIGHT_16_PENALTY=1.029134
PRIMER_LEFT_16_SEQUENCE=AATGGGAAGTGGGATTCAGA
PRIMER_RIGHT_16_SEQUENCE=GAGCACACCTCTCGAACCA
PRIMER_LEFT_16=344,20
PRIMER_RIGHT_16=1074,19
PRIMER_LEFT_16_TM=59.336
PRIMER_RIGHT_16_TM=59.971
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=57.895
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=731
PRIMER_PAIR_16_PRODUCT_TM=80.4
PRIMER_PAIR_17_PENALTY=2.005503
PRIMER_LEFT_17_PENALTY=0.807658
PRIMER_RIGHT_17_PENALTY=1.197845
PRIMER_LEFT_17_SEQUENCE=GATCCATCAGGGACCAAAAC
PRIMER_RIGHT_17_SEQUENCE=TTGTCTTCACTCCCATCTGC
PRIMER_LEFT_17=362,20
PRIMER_RIGHT_17=870,20
PRIMER_LEFT_17_TM=59.192
PRIMER_RIGHT_17_TM=58.802
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=509
PRIMER_PAIR_17_PRODUCT_TM=79.8
PRIMER_PAIR_18_PENALTY=2.063532
PRIMER_LEFT_18_PENALTY=0.663548
PRIMER_RIGHT_18_PENALTY=1.399985
PRIMER_LEFT_18_SEQUENCE=GAATGGGAAGTGGGATTCAG
PRIMER_RIGHT_18_SEQUENCE=CTCACCAACTAAGCAGCGG
PRIMER_LEFT_18=343,20
PRIMER_RIGHT_18=562,19
PRIMER_LEFT_18_TM=59.336
PRIMER_RIGHT_18_TM=59.600
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=57.895
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=11.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=220
PRIMER_PAIR_18_PRODUCT_TM=78.6
PRIMER_PAIR_19_PENALTY=2.075759
PRIMER_LEFT_19_PENALTY=0.828193
PRIMER_RIGHT_19_PENALTY=1.247566
PRIMER_LEFT_19_SEQUENCE=TGGGATTCAGATCCATCAGG
PRIMER_RIGHT_19_SEQUENCE=TTTGTCTTCACTCCCATCTGC
PRIMER_LEFT_19=353,20
PRIMER_RIGHT_19=871,21
PRIMER_LEFT_19_TM=60.828
PRIMER_RIGHT_19_TM=60.248
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=519
PRIMER_PAIR_19_PRODUCT_TM=79.8
PRIMER_PAIR_20_PENALTY=2.092619
PRIMER_LEFT_20_PENALTY=0.663548
PRIMER_RIGHT_20_PENALTY=1.429072
PRIMER_LEFT_20_SEQUENCE=AGAATGGGAAGTGGGATTCA
PRIMER_RIGHT_20_SEQUENCE=AAACTCACCAACTAAGCAGCG
PRIMER_LEFT_20=342,20
PRIMER_RIGHT_20=565,21
PRIMER_LEFT_20_TM=59.336
PRIMER_RIGHT_20_TM=59.571
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=2.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=224
PRIMER_PAIR_20_PRODUCT_TM=78.3
PRIMER_PAIR_21_PENALTY=2.108403
PRIMER_LEFT_21_PENALTY=2.047791
PRIMER_RIGHT_21_PENALTY=0.060612
PRIMER_LEFT_21_SEQUENCE=TTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_21_SEQUENCE=GTTTCGCAAACATCCATCCT
PRIMER_LEFT_21=415,20
PRIMER_RIGHT_21=636,20
PRIMER_LEFT_21_TM=57.952
PRIMER_RIGHT_21_TM=59.939
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=7.8000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=222
PRIMER_PAIR_21_PRODUCT_TM=78.1
PRIMER_PAIR_22_PENALTY=2.110097
PRIMER_LEFT_22_PENALTY=0.663548
PRIMER_RIGHT_22_PENALTY=1.446550
PRIMER_LEFT_22_SEQUENCE=CAGAATGGGAAGTGGGATTC
PRIMER_RIGHT_22_SEQUENCE=TCACCAACTAAGCAGCGGTAG
PRIMER_LEFT_22=341,20
PRIMER_RIGHT_22=561,21
PRIMER_LEFT_22_TM=59.336
PRIMER_RIGHT_22_TM=60.447
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=3.00
PRIMER_RIGHT_22_SELF_END=3.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=221
PRIMER_PAIR_22_PRODUCT_TM=78.5
PRIMER_PAIR_23_PENALTY=2.150754
PRIMER_LEFT_23_PENALTY=0.704204
PRIMER_RIGHT_23_PENALTY=1.446550
PRIMER_LEFT_23_SEQUENCE=CATGAATGTCCAGAATGGGA
PRIMER_RIGHT_23_SEQUENCE=CTCACCAACTAAGCAGCGGTA
PRIMER_LEFT_23=331,20
PRIMER_RIGHT_23=562,21
PRIMER_LEFT_23_TM=59.296
PRIMER_RIGHT_23_TM=60.447
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=2.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=8.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=232
PRIMER_PAIR_23_PRODUCT_TM=78.5
PRIMER_PAIR_24_PENALTY=2.190969
PRIMER_LEFT_24_PENALTY=2.130358
PRIMER_RIGHT_24_PENALTY=0.060612
PRIMER_LEFT_24_SEQUENCE=ATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_24_SEQUENCE=AGTTTCGCAAACATCCATCC
PRIMER_LEFT_24=414,22
PRIMER_RIGHT_24=637,20
PRIMER_LEFT_24_TM=60.130
PRIMER_RIGHT_24_TM=59.939
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=224
PRIMER_PAIR_24_PRODUCT_TM=77.9
PRIMER_PAIR_25_PENALTY=2.333956
PRIMER_LEFT_25_PENALTY=2.096340
PRIMER_RIGHT_25_PENALTY=0.237616
PRIMER_LEFT_25_SEQUENCE=ATCAGCTGACTCGCCTGG
PRIMER_RIGHT_25_SEQUENCE=ACCATCAGTGGGTACCTCCA
PRIMER_LEFT_25=2,18
PRIMER_RIGHT_25=271,20
PRIMER_LEFT_25_TM=60.096
PRIMER_RIGHT_25_TM=60.238
PRIMER_LEFT_25_GC_PERCENT=61.111
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=8.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=3.00
PRIMER_RIGHT_25_SELF_END=2.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=270
PRIMER_PAIR_25_PRODUCT_TM=88.1
PRIMER_PAIR_26_PENALTY=2.396276
PRIMER_LEFT_26_PENALTY=0.923785
PRIMER_RIGHT_26_PENALTY=1.472491
PRIMER_LEFT_26_SEQUENCE=CCCAGATTGCCATGTTCTGT
PRIMER_RIGHT_26_SEQUENCE=TAAACTTTGGGACATGGCG
PRIMER_LEFT_26=294,20
PRIMER_RIGHT_26=1246,19
PRIMER_LEFT_26_TM=60.924
PRIMER_RIGHT_26_TM=59.528
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.368
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=2.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=11.7000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=953
PRIMER_PAIR_26_PRODUCT_TM=80.8
PRIMER_PAIR_27_PENALTY=2.405067
PRIMER_LEFT_27_PENALTY=0.860239
PRIMER_RIGHT_27_PENALTY=1.544828
PRIMER_LEFT_27_SEQUENCE=CAGATCCATCAGGGACCAAA
PRIMER_RIGHT_27_SEQUENCE=TCACCAACTAAGCAGCGGT
PRIMER_LEFT_27=360,20
PRIMER_RIGHT_27=561,19
PRIMER_LEFT_27_TM=60.860
PRIMER_RIGHT_27_TM=59.455
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=52.632
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=2.00
PRIMER_LEFT_27_END_STABILITY=8.8000
PRIMER_RIGHT_27_END_STABILITY=11.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=202
PRIMER_PAIR_27_PRODUCT_TM=78.4
PRIMER_PAIR_28_PENALTY=2.438330
PRIMER_LEFT_28_PENALTY=2.413667
PRIMER_RIGHT_28_PENALTY=0.024663
PRIMER_LEFT_28_SEQUENCE=CGCTGGAGGTACCCACTGAT
PRIMER_RIGHT_28_SEQUENCE=TTGCACTTGTCAGGAACGAG
PRIMER_LEFT_28=249,20
PRIMER_RIGHT_28=600,20
PRIMER_LEFT_28_TM=62.414
PRIMER_RIGHT_28_TM=60.025
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=2.00
PRIMER_RIGHT_28_SELF_END=2.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=352
PRIMER_PAIR_28_PRODUCT_TM=79.5
PRIMER_PAIR_29_PENALTY=2.532768
PRIMER_LEFT_29_PENALTY=0.949861
PRIMER_RIGHT_29_PENALTY=1.582907
PRIMER_LEFT_29_SEQUENCE=ATCCATCAGGGACCAAAACC
PRIMER_RIGHT_29_SEQUENCE=TTCAGAGCACACCTCTCGAAC
PRIMER_LEFT_29=363,20
PRIMER_RIGHT_29=1078,21
PRIMER_LEFT_29_TM=60.950
PRIMER_RIGHT_29_TM=60.583
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=716
PRIMER_PAIR_29_PRODUCT_TM=80.4
PRIMER_PAIR_30_PENALTY=2.686568
PRIMER_LEFT_30_PENALTY=1.014189
PRIMER_RIGHT_30_PENALTY=1.672380
PRIMER_LEFT_30_SEQUENCE=TCAGATCCATCAGGGACCA
PRIMER_RIGHT_30_SEQUENCE=TGTCTTCACTCCCATCTGCAT
PRIMER_LEFT_30=359,19
PRIMER_RIGHT_30=869,21
PRIMER_LEFT_30_TM=59.986
PRIMER_RIGHT_30_TM=60.672
PRIMER_LEFT_30_GC_PERCENT=52.632
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=3.00
PRIMER_RIGHT_30_SELF_END=2.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=511
PRIMER_PAIR_30_PRODUCT_TM=79.8
PRIMER_PAIR_31_PENALTY=2.731923
PRIMER_LEFT_31_PENALTY=2.199316
PRIMER_RIGHT_31_PENALTY=0.532607
PRIMER_LEFT_31_SEQUENCE=GGGCTCCGTCAGTTTCCT
PRIMER_RIGHT_31_SEQUENCE=CATCAGTGGGTACCTCCAGC
PRIMER_LEFT_31=43,18
PRIMER_RIGHT_31=269,20
PRIMER_LEFT_31_TM=60.199
PRIMER_RIGHT_31_TM=60.533
PRIMER_LEFT_31_GC_PERCENT=61.111
PRIMER_RIGHT_31_GC_PERCENT=60.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=2.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=227
PRIMER_PAIR_31_PRODUCT_TM=87.8
PRIMER_PAIR_32_PENALTY=2.732951
PRIMER_LEFT_32_PENALTY=2.660713
PRIMER_RIGHT_32_PENALTY=0.072238
PRIMER_LEFT_32_SEQUENCE=TGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_32_SEQUENCE=CCAGACATCCGAGTCATCCT
PRIMER_LEFT_32=416,22
PRIMER_RIGHT_32=826,20
PRIMER_LEFT_32_TM=60.661
PRIMER_RIGHT_32_TM=60.072
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.8000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=411
PRIMER_PAIR_32_PRODUCT_TM=79.6
PRIMER_PAIR_33_PENALTY=2.741077
PRIMER_LEFT_33_PENALTY=2.668729
PRIMER_RIGHT_33_PENALTY=0.072349
PRIMER_LEFT_33_SEQUENCE=ATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_33_SEQUENCE=ACCAGACATCCGAGTCATCC
PRIMER_LEFT_33=414,21
PRIMER_RIGHT_33=827,20
PRIMER_LEFT_33_TM=58.331
PRIMER_RIGHT_33_TM=59.928
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=8.1000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=414
PRIMER_PAIR_33_PRODUCT_TM=79.4
PRIMER_PAIR_34_PENALTY=2.868697
PRIMER_LEFT_34_PENALTY=1.055809
PRIMER_RIGHT_34_PENALTY=1.812888
PRIMER_LEFT_34_SEQUENCE=CCCAGATTGCCATGTTCTG
PRIMER_RIGHT_34_SEQUENCE=AACTCACCAACTAAGCAGCG
PRIMER_LEFT_34=294,19
PRIMER_RIGHT_34=564,20
PRIMER_LEFT_34_TM=60.056
PRIMER_RIGHT_34_TM=58.187
PRIMER_LEFT_34_GC_PERCENT=52.632
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=2.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=10.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=271
PRIMER_PAIR_34_PRODUCT_TM=78.7
PRIMER_PAIR_35_PENALTY=3.022138
PRIMER_LEFT_35_PENALTY=1.071784
PRIMER_RIGHT_35_PENALTY=1.950354
PRIMER_LEFT_35_SEQUENCE=GATTGCCATGTTCTGTGGC
PRIMER_RIGHT_35_SEQUENCE=CTGCATGTCTCTTTGGCGAC
PRIMER_LEFT_35=298,19
PRIMER_RIGHT_35=675,20
PRIMER_LEFT_35_TM=60.072
PRIMER_RIGHT_35_TM=61.950
PRIMER_LEFT_35_GC_PERCENT=52.632
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=2.00
PRIMER_RIGHT_35_SELF_END=2.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=9.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=378
PRIMER_PAIR_35_PRODUCT_TM=78.9
PRIMER_PAIR_36_PENALTY=3.025966
PRIMER_LEFT_36_PENALTY=1.075611
PRIMER_RIGHT_36_PENALTY=1.950354
PRIMER_LEFT_36_SEQUENCE=CCAGAATGGGAAGTGGGATT
PRIMER_RIGHT_36_SEQUENCE=ACTGCATGTCTCTTTGGCGA
PRIMER_LEFT_36=340,20
PRIMER_RIGHT_36=676,20
PRIMER_LEFT_36_TM=61.076
PRIMER_RIGHT_36_TM=61.950
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=2.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=11.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=337
PRIMER_PAIR_36_PRODUCT_TM=78.9
PRIMER_PAIR_37_PENALTY=3.056272
PRIMER_LEFT_37_PENALTY=1.078278
PRIMER_RIGHT_37_PENALTY=1.977994
PRIMER_LEFT_37_SEQUENCE=AGATTGCCATGTTCTGTGGC
PRIMER_RIGHT_37_SEQUENCE=TCTCACTGCATGTCTCTTTGG
PRIMER_LEFT_37=297,20
PRIMER_RIGHT_37=680,21
PRIMER_LEFT_37_TM=61.078
PRIMER_RIGHT_37_TM=59.022
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=2.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=8.8000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=384
PRIMER_PAIR_37_PRODUCT_TM=78.8
PRIMER_PAIR_38_PENALTY=3.072600
PRIMER_LEFT_38_PENALTY=1.081589
PRIMER_RIGHT_38_PENALTY=1.991012
PRIMER_LEFT_38_SEQUENCE=ATCAGGGACCAAAACCTGC
PRIMER_RIGHT_38_SEQUENCE=CTCACTGCATGTCTCTTTGGC
PRIMER_LEFT_38=367,19
PRIMER_RIGHT_38=679,21
PRIMER_LEFT_38_TM=59.918
PRIMER_RIGHT_38_TM=60.991
PRIMER_LEFT_38_GC_PERCENT=52.632
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=2.00
PRIMER_RIGHT_38_SELF_END=2.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=10.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=313
PRIMER_PAIR_38_PRODUCT_TM=78.8
PRIMER_PAIR_39_PENALTY=3.151416
PRIMER_LEFT_39_PENALTY=3.077802
PRIMER_RIGHT_39_PENALTY=0.073614
PRIMER_LEFT_39_SEQUENCE=GCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_39_SEQUENCE=GTTTAACAGGATCTCGGGCA
PRIMER_LEFT_39=417,21
PRIMER_RIGHT_39=1289,20
PRIMER_LEFT_39_TM=57.922
PRIMER_RIGHT_39_TM=60.074
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=11.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=873
PRIMER_PAIR_39_PRODUCT_TM=81.0
PRIMER_PAIR_40_PENALTY=3.157622
PRIMER_LEFT_40_PENALTY=3.077974
PRIMER_RIGHT_40_PENALTY=0.079648
PRIMER_LEFT_40_SEQUENCE=GCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_40_SEQUENCE=CATCCATCCTCTCCTGGTGT
PRIMER_LEFT_40=417,22
PRIMER_RIGHT_40=626,20
PRIMER_LEFT_40_TM=58.922
PRIMER_RIGHT_40_TM=59.920
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=210
PRIMER_PAIR_40_PRODUCT_TM=78.3
PRIMER_PAIR_41_PENALTY=3.212495
PRIMER_LEFT_41_PENALTY=3.132848
PRIMER_RIGHT_41_PENALTY=0.079648
PRIMER_LEFT_41_SEQUENCE=TTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_41_SEQUENCE=ACATCCATCCTCTCCTGGTG
PRIMER_LEFT_41=415,22
PRIMER_RIGHT_41=627,20
PRIMER_LEFT_41_TM=61.133
PRIMER_RIGHT_41_TM=59.920
PRIMER_LEFT_41_GC_PERCENT=45.455
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=3.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=213
PRIMER_PAIR_41_PRODUCT_TM=78.1
PRIMER_PAIR_42_PENALTY=3.239151
PRIMER_LEFT_42_PENALTY=3.135712
PRIMER_RIGHT_42_PENALTY=0.103439
PRIMER_LEFT_42_SEQUENCE=TATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_42_SEQUENCE=CTACCCCTCGGAACTTGTCA
PRIMER_LEFT_42=413,23
PRIMER_RIGHT_42=746,20
PRIMER_LEFT_42_TM=60.136
PRIMER_RIGHT_42_TM=60.103
PRIMER_LEFT_42_GC_PERCENT=43.478
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=3.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=334
PRIMER_PAIR_42_PRODUCT_TM=79.1
PRIMER_PAIR_43_PENALTY=3.271637
PRIMER_LEFT_43_PENALTY=1.147302
PRIMER_RIGHT_43_PENALTY=2.124336
PRIMER_LEFT_43_SEQUENCE=GGAAGTGGGATTCAGATCCAT
PRIMER_RIGHT_43_SEQUENCE=CTGCATGTCTCTTTGGCGA
PRIMER_LEFT_43=348,21
PRIMER_RIGHT_43=675,19
PRIMER_LEFT_43_TM=60.147
PRIMER_RIGHT_43_TM=61.124
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=52.632
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=2.00
PRIMER_RIGHT_43_SELF_END=2.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=11.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=328
PRIMER_PAIR_43_PRODUCT_TM=78.9
PRIMER_PAIR_44_PENALTY=3.354296
PRIMER_LEFT_44_PENALTY=3.250857
PRIMER_RIGHT_44_PENALTY=0.103439
PRIMER_LEFT_44_SEQUENCE=GCGCTGGAGGTACCCACT
PRIMER_RIGHT_44_SEQUENCE=CACAAACTCTACCCCTCGGA
PRIMER_LEFT_44=248,18
PRIMER_RIGHT_44=754,20
PRIMER_LEFT_44_TM=61.251
PRIMER_RIGHT_44_TM=60.103
PRIMER_LEFT_44_GC_PERCENT=66.667
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=2.00
PRIMER_RIGHT_44_SELF_END=3.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=9.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=507
PRIMER_PAIR_44_PRODUCT_TM=79.8
PRIMER_PAIR_45_PENALTY=3.362562
PRIMER_LEFT_45_PENALTY=1.183296
PRIMER_RIGHT_45_PENALTY=2.179265
PRIMER_LEFT_45_SEQUENCE=AGATCCATCAGGGACCAAAAC
PRIMER_RIGHT_45_SEQUENCE=AGCACACCTCTCGAACCAC
PRIMER_LEFT_45=361,21
PRIMER_RIGHT_45=1073,19
PRIMER_LEFT_45_TM=60.183
PRIMER_RIGHT_45_TM=58.821
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=57.895
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=713
PRIMER_PAIR_45_PRODUCT_TM=80.4
PRIMER_PAIR_46_PENALTY=3.398150
PRIMER_LEFT_46_PENALTY=3.255899
PRIMER_RIGHT_46_PENALTY=0.142251
PRIMER_LEFT_46_SEQUENCE=CGCTGGAGGTACCCACTGA
PRIMER_RIGHT_46_SEQUENCE=AACATGCCGTAGTCATGCAA
PRIMER_LEFT_46=249,19
PRIMER_RIGHT_46=711,20
PRIMER_LEFT_46_TM=62.256
PRIMER_RIGHT_46_TM=60.142
PRIMER_LEFT_46_GC_PERCENT=63.158
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=2.00
PRIMER_RIGHT_46_SELF_END=2.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.8000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=463
PRIMER_PAIR_46_PRODUCT_TM=79.5
PRIMER_PAIR_47_PENALTY=3.428448
PRIMER_LEFT_47_PENALTY=1.188029
PRIMER_RIGHT_47_PENALTY=2.240419
PRIMER_LEFT_47_SEQUENCE=ACATGAATGTCCAGAATGGGA
PRIMER_RIGHT_47_SEQUENCE=ACTCACCAACTAAGCAGCGGT
PRIMER_LEFT_47=330,21
PRIMER_RIGHT_47=563,21
PRIMER_LEFT_47_TM=60.188
PRIMER_RIGHT_47_TM=61.240
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=2.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=11.1000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=234
PRIMER_PAIR_47_PRODUCT_TM=78.4
PRIMER_PAIR_48_PENALTY=3.442199
PRIMER_LEFT_48_PENALTY=1.185045
PRIMER_RIGHT_48_PENALTY=2.257154
PRIMER_LEFT_48_SEQUENCE=GGACCAAAACCTGCATTGATA
PRIMER_RIGHT_48_SEQUENCE=AGTAAACTTTGGGACATGGCG
PRIMER_LEFT_48=372,21
PRIMER_RIGHT_48=1248,21
PRIMER_LEFT_48_TM=59.815
PRIMER_RIGHT_48_TM=61.257
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=2.00
PRIMER_RIGHT_48_SELF_END=2.00
PRIMER_LEFT_48_END_STABILITY=5.9000
PRIMER_RIGHT_48_END_STABILITY=11.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=877
PRIMER_PAIR_48_PRODUCT_TM=80.9
PRIMER_PAIR_49_PENALTY=3.533320
PRIMER_LEFT_49_PENALTY=1.188029
PRIMER_RIGHT_49_PENALTY=2.345290
PRIMER_LEFT_49_SEQUENCE=AACCCCAGATTGCCATGTT
PRIMER_RIGHT_49_SEQUENCE=CACACCTCTCGAACCACCT
PRIMER_LEFT_49=291,19
PRIMER_RIGHT_49=1071,19
PRIMER_LEFT_49_TM=60.188
PRIMER_RIGHT_49_TM=58.655
PRIMER_LEFT_49_GC_PERCENT=47.368
PRIMER_RIGHT_49_GC_PERCENT=57.895
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=2.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.6000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=781
PRIMER_PAIR_49_PRODUCT_TM=80.3
PRIMER_PAIR_50_PENALTY=3.541303
PRIMER_LEFT_50_PENALTY=2.402111
PRIMER_RIGHT_50_PENALTY=1.139192
PRIMER_LEFT_50_SEQUENCE=GGTTTGGCACTGCTCCTG
PRIMER_RIGHT_50_SEQUENCE=TTCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_50=209,18
PRIMER_RIGHT_50=436,21
PRIMER_LEFT_50_TM=60.402
PRIMER_RIGHT_50_TM=59.861
PRIMER_LEFT_50_GC_PERCENT=61.111
PRIMER_RIGHT_50_GC_PERCENT=47.619
PRIMER_LEFT_50_SELF_ANY=5.00
PRIMER_RIGHT_50_SELF_ANY=4.00
PRIMER_LEFT_50_SELF_END=1.00
PRIMER_RIGHT_50_SELF_END=3.00
PRIMER_LEFT_50_END_STABILITY=8.2000
PRIMER_RIGHT_50_END_STABILITY=10.0000
PRIMER_PAIR_50_COMPL_ANY=4.00
PRIMER_PAIR_50_COMPL_END=2.00
PRIMER_PAIR_50_PRODUCT_SIZE=228
PRIMER_PAIR_50_PRODUCT_TM=79.7
PRIMER_PAIR_51_PENALTY=3.591879
PRIMER_LEFT_51_PENALTY=1.236064
PRIMER_RIGHT_51_PENALTY=2.355815
PRIMER_LEFT_51_SEQUENCE=CCATCAGGGACCAAAACCT
PRIMER_RIGHT_51_SEQUENCE=GTCTTCACTCCCATCTGCAT
PRIMER_LEFT_51=365,19
PRIMER_RIGHT_51=868,20
PRIMER_LEFT_51_TM=59.764
PRIMER_RIGHT_51_TM=57.644
PRIMER_LEFT_51_GC_PERCENT=52.632
PRIMER_RIGHT_51_GC_PERCENT=50.000
PRIMER_LEFT_51_SELF_ANY=3.00
PRIMER_RIGHT_51_SELF_ANY=4.00
PRIMER_LEFT_51_SELF_END=0.00
PRIMER_RIGHT_51_SELF_END=2.00
PRIMER_LEFT_51_END_STABILITY=7.9000
PRIMER_RIGHT_51_END_STABILITY=8.4000
PRIMER_PAIR_51_COMPL_ANY=4.00
PRIMER_PAIR_51_COMPL_END=0.00
PRIMER_PAIR_51_PRODUCT_SIZE=504
PRIMER_PAIR_51_PRODUCT_TM=79.9
PRIMER_PAIR_52_PENALTY=3.659168
PRIMER_LEFT_52_PENALTY=3.503107
PRIMER_RIGHT_52_PENALTY=0.156061
PRIMER_LEFT_52_SEQUENCE=GCTGGAGGTACCCACTGAT
PRIMER_RIGHT_52_SEQUENCE=TTCCACCTCAGCCACTTCTT
PRIMER_LEFT_52=250,19
PRIMER_RIGHT_52=913,20
PRIMER_LEFT_52_TM=57.497
PRIMER_RIGHT_52_TM=59.844
PRIMER_LEFT_52_GC_PERCENT=57.895
PRIMER_RIGHT_52_GC_PERCENT=50.000
PRIMER_LEFT_52_SELF_ANY=6.00
PRIMER_RIGHT_52_SELF_ANY=3.00
PRIMER_LEFT_52_SELF_END=2.00
PRIMER_RIGHT_52_SELF_END=0.00
PRIMER_LEFT_52_END_STABILITY=6.6000
PRIMER_RIGHT_52_END_STABILITY=7.0000
PRIMER_PAIR_52_COMPL_ANY=6.00
PRIMER_PAIR_52_COMPL_END=0.00
PRIMER_PAIR_52_PRODUCT_SIZE=664
PRIMER_PAIR_52_PRODUCT_TM=80.1
PRIMER_PAIR_53_PENALTY=3.703097
PRIMER_LEFT_53_PENALTY=1.271611
PRIMER_RIGHT_53_PENALTY=2.431486
PRIMER_LEFT_53_SEQUENCE=GCCATGTTCTGTGGCAGAC
PRIMER_RIGHT_53_SEQUENCE=TTCTCACTGCATGTCTCTTTGG
PRIMER_LEFT_53=302,19
PRIMER_RIGHT_53=681,22
PRIMER_LEFT_53_TM=60.272
PRIMER_RIGHT_53_TM=60.431
PRIMER_LEFT_53_GC_PERCENT=57.895
PRIMER_RIGHT_53_GC_PERCENT=45.455
PRIMER_LEFT_53_SELF_ANY=5.00
PRIMER_RIGHT_53_SELF_ANY=4.00
PRIMER_LEFT_53_SELF_END=3.00
PRIMER_RIGHT_53_SELF_END=0.00
PRIMER_LEFT_53_END_STABILITY=6.4000
PRIMER_RIGHT_53_END_STABILITY=8.8000
PRIMER_PAIR_53_COMPL_ANY=4.00
PRIMER_PAIR_53_COMPL_END=1.00
PRIMER_PAIR_53_PRODUCT_SIZE=380
PRIMER_PAIR_53_PRODUCT_TM=78.9
PRIMER_PAIR_54_PENALTY=3.733965
PRIMER_LEFT_54_PENALTY=3.598066
PRIMER_RIGHT_54_PENALTY=0.135900
PRIMER_LEFT_54_SEQUENCE=TATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_54_SEQUENCE=CGAGATACTTGTCAACGGCA
PRIMER_LEFT_54=413,22
PRIMER_RIGHT_54=1337,20
PRIMER_LEFT_54_TM=58.402
PRIMER_RIGHT_54_TM=59.864
PRIMER_LEFT_54_GC_PERCENT=45.455
PRIMER_RIGHT_54_GC_PERCENT=50.000
PRIMER_LEFT_54_SELF_ANY=5.00
PRIMER_RIGHT_54_SELF_ANY=5.00
PRIMER_LEFT_54_SELF_END=1.00
PRIMER_RIGHT_54_SELF_END=1.00
PRIMER_LEFT_54_END_STABILITY=9.7000
PRIMER_RIGHT_54_END_STABILITY=11.7000
PRIMER_PAIR_54_COMPL_ANY=7.00
PRIMER_PAIR_54_COMPL_END=2.00
PRIMER_PAIR_54_PRODUCT_SIZE=925
PRIMER_PAIR_54_PRODUCT_TM=80.9
PRIMER_PAIR_55_PENALTY=3.734645
PRIMER_LEFT_55_PENALTY=2.495254
PRIMER_RIGHT_55_PENALTY=1.239392
PRIMER_LEFT_55_SEQUENCE=GTCAGTTTCCTCGGCAGC
PRIMER_RIGHT_55_SEQUENCE=TACCATCAGTGGGTACCTCCA
PRIMER_LEFT_55=50,18
PRIMER_RIGHT_55=272,21
PRIMER_LEFT_55_TM=59.505
PRIMER_RIGHT_55_TM=60.239
PRIMER_LEFT_55_GC_PERCENT=61.111
PRIMER_RIGHT_55_GC_PERCENT=52.381
PRIMER_LEFT_55_SELF_ANY=3.00
PRIMER_RIGHT_55_SELF_ANY=6.00
PRIMER_LEFT_55_SELF_END=3.00
PRIMER_RIGHT_55_SELF_END=2.00
PRIMER_LEFT_55_END_STABILITY=9.7000
PRIMER_RIGHT_55_END_STABILITY=8.2000
PRIMER_PAIR_55_COMPL_ANY=3.00
PRIMER_PAIR_55_COMPL_END=0.00
PRIMER_PAIR_55_PRODUCT_SIZE=223
PRIMER_PAIR_55_PRODUCT_TM=87.4
PRIMER_PAIR_56_PENALTY=3.753608
PRIMER_LEFT_56_PENALTY=1.278124
PRIMER_RIGHT_56_PENALTY=2.475484
PRIMER_LEFT_56_SEQUENCE=CCAGAATGGGAAGTGGGAT
PRIMER_RIGHT_56_SEQUENCE=GGCTGCTGTTGTAGGAAGTTT
PRIMER_LEFT_56=340,19
PRIMER_RIGHT_56=1306,21
PRIMER_LEFT_56_TM=59.722
PRIMER_RIGHT_56_TM=58.525
PRIMER_LEFT_56_GC_PERCENT=52.632
PRIMER_RIGHT_56_GC_PERCENT=47.619
PRIMER_LEFT_56_SELF_ANY=3.00
PRIMER_RIGHT_56_SELF_ANY=3.00
PRIMER_LEFT_56_SELF_END=2.00
PRIMER_RIGHT_56_SELF_END=2.00
PRIMER_LEFT_56_END_STABILITY=9.3000
PRIMER_RIGHT_56_END_STABILITY=6.7000
PRIMER_PAIR_56_COMPL_ANY=3.00
PRIMER_PAIR_56_COMPL_END=0.00
PRIMER_PAIR_56_PRODUCT_SIZE=967
PRIMER_PAIR_56_PRODUCT_TM=80.9
PRIMER_PAIR_57_PENALTY=3.795825
PRIMER_LEFT_57_PENALTY=1.292120
PRIMER_RIGHT_57_PENALTY=2.503705
PRIMER_LEFT_57_SEQUENCE=GCCATGTTCTGTGGCAGACT
PRIMER_RIGHT_57_SEQUENCE=AAACTTTGGGACATGGCG
PRIMER_LEFT_57=302,20
PRIMER_RIGHT_57=1245,18
PRIMER_LEFT_57_TM=61.292
PRIMER_RIGHT_57_TM=59.496
PRIMER_LEFT_57_GC_PERCENT=55.000
PRIMER_RIGHT_57_GC_PERCENT=50.000
PRIMER_LEFT_57_SELF_ANY=5.00
PRIMER_RIGHT_57_SELF_ANY=5.00
PRIMER_LEFT_57_SELF_END=3.00
PRIMER_RIGHT_57_SELF_END=2.00
PRIMER_LEFT_57_END_STABILITY=6.1000
PRIMER_RIGHT_57_END_STABILITY=11.7000
PRIMER_PAIR_57_COMPL_ANY=7.00
PRIMER_PAIR_57_COMPL_END=0.00
PRIMER_PAIR_57_PRODUCT_SIZE=944
PRIMER_PAIR_57_PRODUCT_TM=80.8
PRIMER_PAIR_58_PENALTY=3.813730
PRIMER_LEFT_58_PENALTY=3.657669
PRIMER_RIGHT_58_PENALTY=0.156061
PRIMER_LEFT_58_SEQUENCE=GCAGTATTGCCAAGAAGTCTACC
PRIMER_RIGHT_58_SEQUENCE=TTCTTCCACCTCAGCCACTT
PRIMER_LEFT_58=409,23
PRIMER_RIGHT_58=916,20
PRIMER_LEFT_58_TM=59.342
PRIMER_RIGHT_58_TM=59.844
PRIMER_LEFT_58_GC_PERCENT=47.826
PRIMER_RIGHT_58_GC_PERCENT=50.000
PRIMER_LEFT_58_SELF_ANY=6.00
PRIMER_RIGHT_58_SELF_ANY=3.00
PRIMER_LEFT_58_SELF_END=1.00
PRIMER_RIGHT_58_SELF_END=0.00
PRIMER_LEFT_58_END_STABILITY=6.9000
PRIMER_RIGHT_58_END_STABILITY=6.7000
PRIMER_PAIR_58_COMPL_ANY=5.00
PRIMER_PAIR_58_COMPL_END=0.00
PRIMER_PAIR_58_PRODUCT_SIZE=508
PRIMER_PAIR_58_PRODUCT_TM=79.8
PRIMER_PAIR_59_PENALTY=3.884306
PRIMER_LEFT_59_PENALTY=1.309943
PRIMER_RIGHT_59_PENALTY=2.574363
PRIMER_LEFT_59_SEQUENCE=GTCCAGAATGGGAAGTGGG
PRIMER_RIGHT_59_SEQUENCE=TTGTTCAGAGCACACCTCTCG
PRIMER_LEFT_59=338,19
PRIMER_RIGHT_59=1081,21
PRIMER_LEFT_59_TM=60.310
PRIMER_RIGHT_59_TM=61.574
PRIMER_LEFT_59_GC_PERCENT=57.895
PRIMER_RIGHT_59_GC_PERCENT=52.381
PRIMER_LEFT_59_SELF_ANY=3.00
PRIMER_RIGHT_59_SELF_ANY=5.00
PRIMER_LEFT_59_SELF_END=0.00
PRIMER_RIGHT_59_SELF_END=2.00
PRIMER_LEFT_59_END_STABILITY=9.4000
PRIMER_RIGHT_59_END_STABILITY=8.4000
PRIMER_PAIR_59_COMPL_ANY=4.00
PRIMER_PAIR_59_COMPL_END=3.00
PRIMER_PAIR_59_PRODUCT_SIZE=744
PRIMER_PAIR_59_PRODUCT_TM=80.4
PRIMER_PAIR_60_PENALTY=3.967455
PRIMER_LEFT_60_PENALTY=1.312895
PRIMER_RIGHT_60_PENALTY=2.654560
PRIMER_LEFT_60_SEQUENCE=AGAATGGGAAGTGGGATTCAG
PRIMER_RIGHT_60_SEQUENCE=GCACACCTCTCGAACCACCT
PRIMER_LEFT_60=342,21
PRIMER_RIGHT_60=1072,20
PRIMER_LEFT_60_TM=60.313
PRIMER_RIGHT_60_TM=62.655
PRIMER_LEFT_60_GC_PERCENT=47.619
PRIMER_RIGHT_60_GC_PERCENT=60.000
PRIMER_LEFT_60_SELF_ANY=4.00
PRIMER_RIGHT_60_SELF_ANY=4.00
PRIMER_LEFT_60_SELF_END=1.00
PRIMER_RIGHT_60_SELF_END=0.00
PRIMER_LEFT_60_END_STABILITY=7.0000
PRIMER_RIGHT_60_END_STABILITY=7.9000
PRIMER_PAIR_60_COMPL_ANY=4.00
PRIMER_PAIR_60_COMPL_END=3.00
PRIMER_PAIR_60_PRODUCT_SIZE=731
PRIMER_PAIR_60_PRODUCT_TM=80.4
PRIMER_PAIR_61_PENALTY=3.970758
PRIMER_LEFT_61_PENALTY=1.316198
PRIMER_RIGHT_61_PENALTY=2.654560
PRIMER_LEFT_61_SEQUENCE=CCCACTGATGGTAATGCTGG
PRIMER_RIGHT_61_SEQUENCE=AGCACACCTCTCGAACCACC
PRIMER_LEFT_61=260,20
PRIMER_RIGHT_61=1073,20
PRIMER_LEFT_61_TM=61.316
PRIMER_RIGHT_61_TM=62.655
PRIMER_LEFT_61_GC_PERCENT=55.000
PRIMER_RIGHT_61_GC_PERCENT=60.000
PRIMER_LEFT_61_SELF_ANY=3.00
PRIMER_RIGHT_61_SELF_ANY=4.00
PRIMER_LEFT_61_SELF_END=0.00
PRIMER_RIGHT_61_SELF_END=0.00
PRIMER_LEFT_61_END_STABILITY=9.7000
PRIMER_RIGHT_61_END_STABILITY=9.4000
PRIMER_PAIR_61_COMPL_ANY=4.00
PRIMER_PAIR_61_COMPL_END=3.00
PRIMER_PAIR_61_PRODUCT_SIZE=814
PRIMER_PAIR_61_PRODUCT_TM=80.5
PRIMER_PAIR_62_PENALTY=3.989386
PRIMER_LEFT_62_PENALTY=3.791133
PRIMER_RIGHT_62_PENALTY=0.198253
PRIMER_LEFT_62_SEQUENCE=GTATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_62_SEQUENCE=CAGACTCTGTGGTGGTGGTG
PRIMER_LEFT_62=412,23
PRIMER_RIGHT_62=1046,20
PRIMER_LEFT_62_TM=59.209
PRIMER_RIGHT_62_TM=60.198
PRIMER_LEFT_62_GC_PERCENT=47.826
PRIMER_RIGHT_62_GC_PERCENT=60.000
PRIMER_LEFT_62_SELF_ANY=5.00
PRIMER_RIGHT_62_SELF_ANY=7.00
PRIMER_LEFT_62_SELF_END=1.00
PRIMER_RIGHT_62_SELF_END=0.00
PRIMER_LEFT_62_END_STABILITY=9.7000
PRIMER_RIGHT_62_END_STABILITY=8.2000
PRIMER_PAIR_62_COMPL_ANY=6.00
PRIMER_PAIR_62_COMPL_END=0.00
PRIMER_PAIR_62_PRODUCT_SIZE=635
PRIMER_PAIR_62_PRODUCT_TM=80.3
PRIMER_PAIR_63_PENALTY=3.996838
PRIMER_LEFT_63_PENALTY=3.791133
PRIMER_RIGHT_63_PENALTY=0.205704
PRIMER_LEFT_63_SEQUENCE=CAGTATTGCCAAGAAGTCTACCC
PRIMER_RIGHT_63_SEQUENCE=TTAACAGGATCTCGGGCAAG
PRIMER_LEFT_63=410,23
PRIMER_RIGHT_63=1287,20
PRIMER_LEFT_63_TM=59.209
PRIMER_RIGHT_63_TM=60.206
PRIMER_LEFT_63_GC_PERCENT=47.826
PRIMER_RIGHT_63_GC_PERCENT=50.000
PRIMER_LEFT_63_SELF_ANY=5.00
PRIMER_RIGHT_63_SELF_ANY=4.00
PRIMER_LEFT_63_SELF_END=0.00
PRIMER_RIGHT_63_SELF_END=2.00
PRIMER_LEFT_63_END_STABILITY=8.4000
PRIMER_RIGHT_63_END_STABILITY=8.5000
PRIMER_PAIR_63_COMPL_ANY=5.00
PRIMER_PAIR_63_COMPL_END=2.00
PRIMER_PAIR_63_PRODUCT_SIZE=878
PRIMER_PAIR_63_PRODUCT_TM=80.9
PRIMER_PAIR_64_PENALTY=4.059135
PRIMER_LEFT_64_PENALTY=2.500904
PRIMER_RIGHT_64_PENALTY=1.558232
PRIMER_LEFT_64_SEQUENCE=GCTCCGTCAGTTTCCTCG
PRIMER_RIGHT_64_SEQUENCE=TCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_64=45,18
PRIMER_RIGHT_64=435,20
PRIMER_LEFT_64_TM=59.499
PRIMER_RIGHT_64_TM=58.442
PRIMER_LEFT_64_GC_PERCENT=61.111
PRIMER_RIGHT_64_GC_PERCENT=50.000
PRIMER_LEFT_64_SELF_ANY=2.00
PRIMER_RIGHT_64_SELF_ANY=4.00
PRIMER_LEFT_64_SELF_END=2.00
PRIMER_RIGHT_64_SELF_END=3.00
PRIMER_LEFT_64_END_STABILITY=9.9000
PRIMER_RIGHT_64_END_STABILITY=10.0000
PRIMER_PAIR_64_COMPL_ANY=3.00
PRIMER_PAIR_64_COMPL_END=1.00
PRIMER_PAIR_64_PRODUCT_SIZE=391
PRIMER_PAIR_64_PRODUCT_TM=84.6
PRIMER_PAIR_65_PENALTY=4.064642
PRIMER_LEFT_65_PENALTY=1.392601
PRIMER_RIGHT_65_PENALTY=2.672041
PRIMER_LEFT_65_SEQUENCE=TCCTGCAGTATTGCCAAGAAG
PRIMER_RIGHT_65_SEQUENCE=CACACCTCTCGAACCACCTCT
PRIMER_LEFT_65=405,21
PRIMER_RIGHT_65=1071,21
PRIMER_LEFT_65_TM=60.393
PRIMER_RIGHT_65_TM=61.672
PRIMER_LEFT_65_GC_PERCENT=47.619
PRIMER_RIGHT_65_GC_PERCENT=57.143
PRIMER_LEFT_65_SELF_ANY=6.00
PRIMER_RIGHT_65_SELF_ANY=4.00
PRIMER_LEFT_65_SELF_END=1.00
PRIMER_RIGHT_65_SELF_END=0.00
PRIMER_LEFT_65_END_STABILITY=6.7000
PRIMER_RIGHT_65_END_STABILITY=7.9000
PRIMER_PAIR_65_COMPL_ANY=3.00
PRIMER_PAIR_65_COMPL_END=3.00
PRIMER_PAIR_65_PRODUCT_SIZE=667
PRIMER_PAIR_65_PRODUCT_TM=80.5
PRIMER_PAIR_66_PENALTY=4.067344
PRIMER_LEFT_66_PENALTY=1.357294
PRIMER_RIGHT_66_PENALTY=2.710050
PRIMER_LEFT_66_SEQUENCE=AATGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_66_SEQUENCE=AACTCACCAACTAAGCAGCGG
PRIMER_LEFT_66=344,21
PRIMER_RIGHT_66=564,21
PRIMER_LEFT_66_TM=59.643
PRIMER_RIGHT_66_TM=61.710
PRIMER_LEFT_66_GC_PERCENT=42.857
PRIMER_RIGHT_66_GC_PERCENT=52.381
PRIMER_LEFT_66_SELF_ANY=3.00
PRIMER_RIGHT_66_SELF_ANY=3.00
PRIMER_LEFT_66_SELF_END=2.00
PRIMER_RIGHT_66_SELF_END=2.00
PRIMER_LEFT_66_END_STABILITY=6.6000
PRIMER_RIGHT_66_END_STABILITY=11.4000
PRIMER_PAIR_66_COMPL_ANY=4.00
PRIMER_PAIR_66_COMPL_END=1.00
PRIMER_PAIR_66_PRODUCT_SIZE=221
PRIMER_PAIR_66_PRODUCT_TM=78.3
PRIMER_PAIR_67_PENALTY=4.156276
PRIMER_LEFT_67_PENALTY=1.427808
PRIMER_RIGHT_67_PENALTY=2.728469
PRIMER_LEFT_67_SEQUENCE=CATTGATACCAAGGAAGGCA
PRIMER_RIGHT_67_SEQUENCE=GCACACCTCTCGAACCACC
PRIMER_LEFT_67=385,20
PRIMER_RIGHT_67=1072,19
PRIMER_LEFT_67_TM=58.572
PRIMER_RIGHT_67_TM=61.728
PRIMER_LEFT_67_GC_PERCENT=45.000
PRIMER_RIGHT_67_GC_PERCENT=63.158
PRIMER_LEFT_67_SELF_ANY=6.00
PRIMER_RIGHT_67_SELF_ANY=4.00
PRIMER_LEFT_67_SELF_END=0.00
PRIMER_RIGHT_67_SELF_END=0.00
PRIMER_LEFT_67_END_STABILITY=9.7000
PRIMER_RIGHT_67_END_STABILITY=9.4000
PRIMER_PAIR_67_COMPL_ANY=3.00
PRIMER_PAIR_67_COMPL_END=0.00
PRIMER_PAIR_67_PRODUCT_SIZE=688
PRIMER_PAIR_67_PRODUCT_TM=80.4
PRIMER_PAIR_68_PENALTY=4.222413
PRIMER_LEFT_68_PENALTY=2.546814
PRIMER_RIGHT_68_PENALTY=1.675600
PRIMER_LEFT_68_SEQUENCE=CGTCAGTTTCCTCGGCAG
PRIMER_RIGHT_68_SEQUENCE=CCATCAGTGGGTACCTCCA
PRIMER_LEFT_68=49,18
PRIMER_RIGHT_68=270,19
PRIMER_LEFT_68_TM=60.547
PRIMER_RIGHT_68_TM=59.324
PRIMER_LEFT_68_GC_PERCENT=61.111
PRIMER_RIGHT_68_GC_PERCENT=57.895
PRIMER_LEFT_68_SELF_ANY=2.00
PRIMER_RIGHT_68_SELF_ANY=6.00
PRIMER_LEFT_68_SELF_END=2.00
PRIMER_RIGHT_68_SELF_END=2.00
PRIMER_LEFT_68_END_STABILITY=9.7000
PRIMER_RIGHT_68_END_STABILITY=8.2000
PRIMER_PAIR_68_COMPL_ANY=3.00
PRIMER_PAIR_68_COMPL_END=1.00
PRIMER_PAIR_68_PRODUCT_SIZE=222
PRIMER_PAIR_68_PRODUCT_TM=87.7
PRIMER_PAIR_69_PENALTY=4.245946
PRIMER_LEFT_69_PENALTY=4.008330
PRIMER_RIGHT_69_PENALTY=0.237616
PRIMER_LEFT_69_SEQUENCE=CCAAGAAGTCTACCCTGAACTG
PRIMER_RIGHT_69_SEQUENCE=TCTCGAACCACCTCTTCCAC
PRIMER_LEFT_69=418,22
PRIMER_RIGHT_69=1065,20
PRIMER_LEFT_69_TM=57.992
PRIMER_RIGHT_69_TM=60.238
PRIMER_LEFT_69_GC_PERCENT=50.000
PRIMER_RIGHT_69_GC_PERCENT=55.000
PRIMER_LEFT_69_SELF_ANY=4.00
PRIMER_RIGHT_69_SELF_ANY=4.00
PRIMER_LEFT_69_SELF_END=1.00
PRIMER_RIGHT_69_SELF_END=0.00
PRIMER_LEFT_69_END_STABILITY=6.7000
PRIMER_RIGHT_69_END_STABILITY=7.9000
PRIMER_PAIR_69_COMPL_ANY=5.00
PRIMER_PAIR_69_COMPL_END=1.00
PRIMER_PAIR_69_PRODUCT_SIZE=648
PRIMER_PAIR_69_PRODUCT_TM=80.4
PRIMER_PAIR_70_PENALTY=4.251723
PRIMER_LEFT_70_PENALTY=1.427808
PRIMER_RIGHT_70_PENALTY=2.823915
PRIMER_LEFT_70_SEQUENCE=TGCATTGATACCAAGGAAGG
PRIMER_RIGHT_70_SEQUENCE=AGAGCACACCTCTCGAACCAC
PRIMER_LEFT_70=383,20
PRIMER_RIGHT_70=1075,21
PRIMER_LEFT_70_TM=58.572
PRIMER_RIGHT_70_TM=61.824
PRIMER_LEFT_70_GC_PERCENT=45.000
PRIMER_RIGHT_70_GC_PERCENT=57.143
PRIMER_LEFT_70_SELF_ANY=6.00
PRIMER_RIGHT_70_SELF_ANY=4.00
PRIMER_LEFT_70_SELF_END=0.00
PRIMER_RIGHT_70_SELF_END=0.00
PRIMER_LEFT_70_END_STABILITY=8.2000
PRIMER_RIGHT_70_END_STABILITY=7.6000
PRIMER_PAIR_70_COMPL_ANY=3.00
PRIMER_PAIR_70_COMPL_END=1.00
PRIMER_PAIR_70_PRODUCT_SIZE=693
PRIMER_PAIR_70_PRODUCT_TM=80.4
PRIMER_PAIR_71_PENALTY=4.316825
PRIMER_LEFT_71_PENALTY=1.440351
PRIMER_RIGHT_71_PENALTY=2.876473
PRIMER_LEFT_71_SEQUENCE=TCAGATCCATCAGGGACCAA
PRIMER_RIGHT_71_SEQUENCE=TCAGAGCACACCTCTCGAA
PRIMER_LEFT_71=359,20
PRIMER_RIGHT_71=1077,19
PRIMER_LEFT_71_TM=61.440
PRIMER_RIGHT_71_TM=58.124
PRIMER_LEFT_71_GC_PERCENT=50.000
PRIMER_RIGHT_71_GC_PERCENT=52.632
PRIMER_LEFT_71_SELF_ANY=5.00
PRIMER_RIGHT_71_SELF_ANY=4.00
PRIMER_LEFT_71_SELF_END=1.00
PRIMER_RIGHT_71_SELF_END=2.00
PRIMER_LEFT_71_END_STABILITY=8.2000
PRIMER_RIGHT_71_END_STABILITY=8.7000
PRIMER_PAIR_71_COMPL_ANY=3.00
PRIMER_PAIR_71_COMPL_END=0.00
PRIMER_PAIR_71_PRODUCT_SIZE=719
PRIMER_PAIR_71_PRODUCT_TM=80.4
PRIMER_PAIR_72_PENALTY=4.329868
PRIMER_LEFT_72_PENALTY=4.076046
PRIMER_RIGHT_72_PENALTY=0.253823
PRIMER_LEFT_72_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_72_SEQUENCE=CATGCCGTAGTCATGCAAGT
PRIMER_LEFT_72=411,24
PRIMER_RIGHT_72=709,20
PRIMER_LEFT_72_TM=60.076
PRIMER_RIGHT_72_TM=59.746
PRIMER_LEFT_72_GC_PERCENT=45.833
PRIMER_RIGHT_72_GC_PERCENT=50.000
PRIMER_LEFT_72_SELF_ANY=5.00
PRIMER_RIGHT_72_SELF_ANY=6.00
PRIMER_LEFT_72_SELF_END=1.00
PRIMER_RIGHT_72_SELF_END=2.00
PRIMER_LEFT_72_END_STABILITY=9.7000
PRIMER_RIGHT_72_END_STABILITY=6.7000
PRIMER_PAIR_72_COMPL_ANY=4.00
PRIMER_PAIR_72_COMPL_END=1.00
PRIMER_PAIR_72_PRODUCT_SIZE=299
PRIMER_PAIR_72_PRODUCT_TM=78.6
PRIMER_PAIR_73_PENALTY=4.360420
PRIMER_LEFT_73_PENALTY=4.076046
PRIMER_RIGHT_73_PENALTY=0.284374
PRIMER_LEFT_73_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCT
PRIMER_RIGHT_73_SEQUENCE=TCACATCAAAGTACCAGCGG
PRIMER_LEFT_73=410,24
PRIMER_RIGHT_73=1133,20
PRIMER_LEFT_73_TM=60.076
PRIMER_RIGHT_73_TM=59.716
PRIMER_LEFT_73_GC_PERCENT=45.833
PRIMER_RIGHT_73_GC_PERCENT=50.000
PRIMER_LEFT_73_SELF_ANY=5.00
PRIMER_RIGHT_73_SELF_ANY=4.00
PRIMER_LEFT_73_SELF_END=2.00
PRIMER_RIGHT_73_SELF_END=2.00
PRIMER_LEFT_73_END_STABILITY=9.1000
PRIMER_RIGHT_73_END_STABILITY=11.4000
PRIMER_PAIR_73_COMPL_ANY=3.00
PRIMER_PAIR_73_COMPL_END=2.00
PRIMER_PAIR_73_PRODUCT_SIZE=724
PRIMER_PAIR_73_PRODUCT_TM=80.7
PRIMER_PAIR_74_PENALTY=4.383084
PRIMER_LEFT_74_PENALTY=1.440351
PRIMER_RIGHT_74_PENALTY=2.942733
PRIMER_LEFT_74_SEQUENCE=TTCAGATCCATCAGGGACCA
PRIMER_RIGHT_74_SEQUENCE=CTGCATGTCTCTTTGGCG
PRIMER_LEFT_74=358,20
PRIMER_RIGHT_74=675,18
PRIMER_LEFT_74_TM=61.440
PRIMER_RIGHT_74_TM=59.057
PRIMER_LEFT_74_GC_PERCENT=50.000
PRIMER_RIGHT_74_GC_PERCENT=55.556
PRIMER_LEFT_74_SELF_ANY=5.00
PRIMER_RIGHT_74_SELF_ANY=4.00
PRIMER_LEFT_74_SELF_END=3.00
PRIMER_RIGHT_74_SELF_END=2.00
PRIMER_LEFT_74_END_STABILITY=7.9000
PRIMER_RIGHT_74_END_STABILITY=11.7000
PRIMER_PAIR_74_COMPL_ANY=5.00
PRIMER_PAIR_74_COMPL_END=1.00
PRIMER_PAIR_74_PRODUCT_SIZE=318
PRIMER_PAIR_74_PRODUCT_TM=78.8
PRIMER_PAIR_75_PENALTY=4.416017
PRIMER_LEFT_75_PENALTY=1.468408
PRIMER_RIGHT_75_PENALTY=2.947608
PRIMER_LEFT_75_SEQUENCE=CCACTGATGGTAATGCTGGC
PRIMER_RIGHT_75_SEQUENCE=CACTGCATGTCTCTTTGGCG
PRIMER_LEFT_75=261,20
PRIMER_RIGHT_75=677,20
PRIMER_LEFT_75_TM=61.468
PRIMER_RIGHT_75_TM=62.948
PRIMER_LEFT_75_GC_PERCENT=55.000
PRIMER_RIGHT_75_GC_PERCENT=55.000
PRIMER_LEFT_75_SELF_ANY=3.00
PRIMER_RIGHT_75_SELF_ANY=4.00
PRIMER_LEFT_75_SELF_END=2.00
PRIMER_RIGHT_75_SELF_END=2.00
PRIMER_LEFT_75_END_STABILITY=9.7000
PRIMER_RIGHT_75_END_STABILITY=11.7000
PRIMER_PAIR_75_COMPL_ANY=4.00
PRIMER_PAIR_75_COMPL_END=1.00
PRIMER_PAIR_75_PRODUCT_SIZE=417
PRIMER_PAIR_75_PRODUCT_TM=79.4
PRIMER_PAIR_76_PENALTY=4.519707
PRIMER_LEFT_76_PENALTY=4.253992
PRIMER_RIGHT_76_PENALTY=0.265715
PRIMER_LEFT_76_SEQUENCE=TGCAGTATTGCCAAGAAGTCTA
PRIMER_RIGHT_76_SEQUENCE=TGTGGCTTCTTCGTAGGGTT
PRIMER_LEFT_76=408,22
PRIMER_RIGHT_76=997,20
PRIMER_LEFT_76_TM=57.746
PRIMER_RIGHT_76_TM=59.734
PRIMER_LEFT_76_GC_PERCENT=40.909
PRIMER_RIGHT_76_GC_PERCENT=50.000
PRIMER_LEFT_76_SELF_ANY=6.00
PRIMER_RIGHT_76_SELF_ANY=4.00
PRIMER_LEFT_76_SELF_END=2.00
PRIMER_RIGHT_76_SELF_END=0.00
PRIMER_LEFT_76_END_STABILITY=5.4000
PRIMER_RIGHT_76_END_STABILITY=9.4000
PRIMER_PAIR_76_COMPL_ANY=6.00
PRIMER_PAIR_76_COMPL_END=3.00
PRIMER_PAIR_76_PRODUCT_SIZE=590
PRIMER_PAIR_76_PRODUCT_TM=80.0
PRIMER_PAIR_77_PENALTY=4.540067
PRIMER_LEFT_77_PENALTY=1.472425
PRIMER_RIGHT_77_PENALTY=3.067642
PRIMER_LEFT_77_SEQUENCE=CCTGCAGTATTGCCAAGAAG
PRIMER_RIGHT_77_SEQUENCE=AGAGCACACCTCTCGAACC
PRIMER_LEFT_77=406,20
PRIMER_RIGHT_77=1075,19
PRIMER_LEFT_77_TM=58.528
PRIMER_RIGHT_77_TM=57.932
PRIMER_LEFT_77_GC_PERCENT=50.000
PRIMER_RIGHT_77_GC_PERCENT=57.895
PRIMER_LEFT_77_SELF_ANY=6.00
PRIMER_RIGHT_77_SELF_ANY=4.00
PRIMER_LEFT_77_SELF_END=1.00
PRIMER_RIGHT_77_SELF_END=0.00
PRIMER_LEFT_77_END_STABILITY=6.7000
PRIMER_RIGHT_77_END_STABILITY=7.9000
PRIMER_PAIR_77_COMPL_ANY=3.00
PRIMER_PAIR_77_COMPL_END=1.00
PRIMER_PAIR_77_PRODUCT_SIZE=670
PRIMER_PAIR_77_PRODUCT_TM=80.5
PRIMER_PAIR_78_PENALTY=4.550427
PRIMER_LEFT_78_PENALTY=4.266053
PRIMER_RIGHT_78_PENALTY=0.284374
PRIMER_LEFT_78_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTA
PRIMER_RIGHT_78_SEQUENCE=TACTTGTCAACGGCATCAGG
PRIMER_LEFT_78=407,23
PRIMER_RIGHT_78=1332,20
PRIMER_LEFT_78_TM=58.734
PRIMER_RIGHT_78_TM=59.716
PRIMER_LEFT_78_GC_PERCENT=43.478
PRIMER_RIGHT_78_GC_PERCENT=50.000
PRIMER_LEFT_78_SELF_ANY=6.00
PRIMER_RIGHT_78_SELF_ANY=5.00
PRIMER_LEFT_78_SELF_END=2.00
PRIMER_RIGHT_78_SELF_END=1.00
PRIMER_LEFT_78_END_STABILITY=5.4000
PRIMER_RIGHT_78_END_STABILITY=8.2000
PRIMER_PAIR_78_COMPL_ANY=6.00
PRIMER_PAIR_78_COMPL_END=2.00
PRIMER_PAIR_78_PRODUCT_SIZE=926
PRIMER_PAIR_78_PRODUCT_TM=80.9
PRIMER_PAIR_79_PENALTY=4.611847
PRIMER_LEFT_79_PENALTY=4.326727
PRIMER_RIGHT_79_PENALTY=0.285121
PRIMER_LEFT_79_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_79_SEQUENCE=ACGGCCATGCAGTACTCTTC
PRIMER_LEFT_79=414,23
PRIMER_RIGHT_79=1218,20
PRIMER_LEFT_79_TM=61.327
PRIMER_RIGHT_79_TM=60.285
PRIMER_LEFT_79_GC_PERCENT=43.478
PRIMER_RIGHT_79_GC_PERCENT=55.000
PRIMER_LEFT_79_SELF_ANY=5.00
PRIMER_RIGHT_79_SELF_ANY=6.00
PRIMER_LEFT_79_SELF_END=0.00
PRIMER_RIGHT_79_SELF_END=2.00
PRIMER_LEFT_79_END_STABILITY=7.0000
PRIMER_RIGHT_79_END_STABILITY=6.7000
PRIMER_PAIR_79_COMPL_ANY=5.00
PRIMER_PAIR_79_COMPL_END=3.00
PRIMER_PAIR_79_PRODUCT_SIZE=805
PRIMER_PAIR_79_PRODUCT_TM=80.9
PRIMER_PAIR_80_PENALTY=4.614912
PRIMER_LEFT_80_PENALTY=1.512710
PRIMER_RIGHT_80_PENALTY=3.102202
PRIMER_LEFT_80_SEQUENCE=TGAATGTCCAGAATGGGAAG
PRIMER_RIGHT_80_SEQUENCE=CACTGCATGTCTCTTTGGC
PRIMER_LEFT_80=333,20
PRIMER_RIGHT_80=677,19
PRIMER_LEFT_80_TM=58.487
PRIMER_RIGHT_80_TM=57.898
PRIMER_LEFT_80_GC_PERCENT=45.000
PRIMER_RIGHT_80_GC_PERCENT=52.632
PRIMER_LEFT_80_SELF_ANY=3.00
PRIMER_RIGHT_80_SELF_ANY=4.00
PRIMER_LEFT_80_SELF_END=0.00
PRIMER_RIGHT_80_SELF_END=2.00
PRIMER_LEFT_80_END_STABILITY=8.2000
PRIMER_RIGHT_80_END_STABILITY=10.0000
PRIMER_PAIR_80_COMPL_ANY=4.00
PRIMER_PAIR_80_COMPL_END=1.00
PRIMER_PAIR_80_PRODUCT_SIZE=345
PRIMER_PAIR_80_PRODUCT_TM=78.8
PRIMER_PAIR_81_PENALTY=4.689041
PRIMER_LEFT_81_PENALTY=4.399603
PRIMER_RIGHT_81_PENALTY=0.289438
PRIMER_LEFT_81_SEQUENCE=TGCAGTATTGCCAAGAAGTCTAC
PRIMER_RIGHT_81_SEQUENCE=GCATGTTCATTCTCATCCCC
PRIMER_LEFT_81=408,23
PRIMER_RIGHT_81=1365,20
PRIMER_LEFT_81_TM=58.600
PRIMER_RIGHT_81_TM=60.289
PRIMER_LEFT_81_GC_PERCENT=43.478
PRIMER_RIGHT_81_GC_PERCENT=50.000
PRIMER_LEFT_81_SELF_ANY=6.00
PRIMER_RIGHT_81_SELF_ANY=4.00
PRIMER_LEFT_81_SELF_END=3.00
PRIMER_RIGHT_81_SELF_END=0.00
PRIMER_LEFT_81_END_STABILITY=5.4000
PRIMER_RIGHT_81_END_STABILITY=10.9000
PRIMER_PAIR_81_COMPL_ANY=4.00
PRIMER_PAIR_81_COMPL_END=0.00
PRIMER_PAIR_81_PRODUCT_SIZE=958
PRIMER_PAIR_81_PRODUCT_TM=80.9
PRIMER_PAIR_82_PENALTY=4.693533
PRIMER_LEFT_82_PENALTY=2.563176
PRIMER_RIGHT_82_PENALTY=2.130358
PRIMER_LEFT_82_SEQUENCE=GTTTGGCACTGCTCCTGCT
PRIMER_RIGHT_82_SEQUENCE=TCAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_82=210,19
PRIMER_RIGHT_82=435,22
PRIMER_LEFT_82_TM=61.563
PRIMER_RIGHT_82_TM=60.130
PRIMER_LEFT_82_GC_PERCENT=57.895
PRIMER_RIGHT_82_GC_PERCENT=45.455
PRIMER_LEFT_82_SELF_ANY=5.00
PRIMER_RIGHT_82_SELF_ANY=5.00
PRIMER_LEFT_82_SELF_END=0.00
PRIMER_RIGHT_82_SELF_END=3.00
PRIMER_LEFT_82_END_STABILITY=8.2000
PRIMER_RIGHT_82_END_STABILITY=8.4000
PRIMER_PAIR_82_COMPL_ANY=4.00
PRIMER_PAIR_82_COMPL_END=0.00
PRIMER_PAIR_82_PRODUCT_SIZE=226
PRIMER_PAIR_82_PRODUCT_TM=79.7
PRIMER_PAIR_83_PENALTY=4.693889
PRIMER_LEFT_83_PENALTY=1.513670
PRIMER_RIGHT_83_PENALTY=3.180219
PRIMER_LEFT_83_SEQUENCE=GGACCAAAACCTGCATTGA
PRIMER_RIGHT_83_SEQUENCE=CTTTGTCTTCACTCCCATCTGC
PRIMER_LEFT_83=372,19
PRIMER_RIGHT_83=872,22
PRIMER_LEFT_83_TM=59.486
PRIMER_RIGHT_83_TM=61.180
PRIMER_LEFT_83_GC_PERCENT=47.368
PRIMER_RIGHT_83_GC_PERCENT=50.000
PRIMER_LEFT_83_SELF_ANY=4.00
PRIMER_RIGHT_83_SELF_ANY=2.00
PRIMER_LEFT_83_SELF_END=1.00
PRIMER_RIGHT_83_SELF_END=2.00
PRIMER_LEFT_83_END_STABILITY=6.9000
PRIMER_RIGHT_83_END_STABILITY=8.2000
PRIMER_PAIR_83_COMPL_ANY=4.00
PRIMER_PAIR_83_COMPL_END=1.00
PRIMER_PAIR_83_PRODUCT_SIZE=501
PRIMER_PAIR_83_PRODUCT_TM=79.8
PRIMER_PAIR_84_PENALTY=4.715844
PRIMER_LEFT_84_PENALTY=1.532916
PRIMER_RIGHT_84_PENALTY=3.182928
PRIMER_LEFT_84_SEQUENCE=TGCACATGAATGTCCAGAATG
PRIMER_RIGHT_84_SEQUENCE=GTCTTCACTCCCATCTGCATAG
PRIMER_LEFT_84=327,21
PRIMER_RIGHT_84=868,22
PRIMER_LEFT_84_TM=60.533
PRIMER_RIGHT_84_TM=58.817
PRIMER_LEFT_84_GC_PERCENT=42.857
PRIMER_RIGHT_84_GC_PERCENT=50.000
PRIMER_LEFT_84_SELF_ANY=8.00
PRIMER_RIGHT_84_SELF_ANY=4.00
PRIMER_LEFT_84_SELF_END=1.00
PRIMER_RIGHT_84_SELF_END=2.00
PRIMER_LEFT_84_END_STABILITY=6.9000
PRIMER_RIGHT_84_END_STABILITY=5.9000
PRIMER_PAIR_84_COMPL_ANY=6.00
PRIMER_PAIR_84_COMPL_END=3.00
PRIMER_PAIR_84_PRODUCT_SIZE=542
PRIMER_PAIR_84_PRODUCT_TM=79.8
PRIMER_PAIR_85_PENALTY=4.720614
PRIMER_LEFT_85_PENALTY=1.532916
PRIMER_RIGHT_85_PENALTY=3.187697
PRIMER_LEFT_85_SEQUENCE=CATGCACATGAATGTCCAGAA
PRIMER_RIGHT_85_SEQUENCE=TGGCTGCTGTTGTAGGAAGTTT
PRIMER_LEFT_85=325,21
PRIMER_RIGHT_85=1307,22
PRIMER_LEFT_85_TM=60.533
PRIMER_RIGHT_85_TM=61.188
PRIMER_LEFT_85_GC_PERCENT=42.857
PRIMER_RIGHT_85_GC_PERCENT=45.455
PRIMER_LEFT_85_SELF_ANY=8.00
PRIMER_RIGHT_85_SELF_ANY=3.00
PRIMER_LEFT_85_SELF_END=3.00
PRIMER_RIGHT_85_SELF_END=2.00
PRIMER_LEFT_85_END_STABILITY=7.0000
PRIMER_RIGHT_85_END_STABILITY=6.7000
PRIMER_PAIR_85_COMPL_ANY=3.00
PRIMER_PAIR_85_COMPL_END=2.00
PRIMER_PAIR_85_PRODUCT_SIZE=983
PRIMER_PAIR_85_PRODUCT_TM=80.8
PRIMER_PAIR_86_PENALTY=4.748900
PRIMER_LEFT_86_PENALTY=1.543091
PRIMER_RIGHT_86_PENALTY=3.205809
PRIMER_LEFT_86_SEQUENCE=TGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_86_SEQUENCE=ACTCACCAACTAAGCAGCGGTA
PRIMER_LEFT_86=337,19
PRIMER_RIGHT_86=563,22
PRIMER_LEFT_86_TM=59.457
PRIMER_RIGHT_86_TM=61.206
PRIMER_LEFT_86_GC_PERCENT=52.632
PRIMER_RIGHT_86_GC_PERCENT=50.000
PRIMER_LEFT_86_SELF_ANY=3.00
PRIMER_RIGHT_86_SELF_ANY=3.00
PRIMER_LEFT_86_SELF_END=0.00
PRIMER_RIGHT_86_SELF_END=2.00
PRIMER_LEFT_86_END_STABILITY=7.9000
PRIMER_RIGHT_86_END_STABILITY=8.9000
PRIMER_PAIR_86_COMPL_ANY=4.00
PRIMER_PAIR_86_COMPL_END=0.00
PRIMER_PAIR_86_PRODUCT_SIZE=227
PRIMER_PAIR_86_PRODUCT_TM=78.6
PRIMER_PAIR_87_PENALTY=4.772082
PRIMER_LEFT_87_PENALTY=4.482644
PRIMER_RIGHT_87_PENALTY=0.289438
PRIMER_LEFT_87_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTAC
PRIMER_RIGHT_87_SEQUENCE=GGGCATGTTCATTCTCATCC
PRIMER_LEFT_87=407,24
PRIMER_RIGHT_87=1367,20
PRIMER_LEFT_87_TM=59.517
PRIMER_RIGHT_87_TM=60.289
PRIMER_LEFT_87_GC_PERCENT=45.833
PRIMER_RIGHT_87_GC_PERCENT=50.000
PRIMER_LEFT_87_SELF_ANY=6.00
PRIMER_RIGHT_87_SELF_ANY=4.00
PRIMER_LEFT_87_SELF_END=3.00
PRIMER_RIGHT_87_SELF_END=2.00
PRIMER_LEFT_87_END_STABILITY=5.4000
PRIMER_RIGHT_87_END_STABILITY=8.1000
PRIMER_PAIR_87_COMPL_ANY=4.00
PRIMER_PAIR_87_COMPL_END=0.00
PRIMER_PAIR_87_PRODUCT_SIZE=961
PRIMER_PAIR_87_PRODUCT_TM=81.0
PRIMER_PAIR_88_PENALTY=4.801409
PRIMER_LEFT_88_PENALTY=4.514227
PRIMER_RIGHT_88_PENALTY=0.287182
PRIMER_LEFT_88_SEQUENCE=CCAAGAAGTCTACCCTGAACTGC
PRIMER_RIGHT_88_SEQUENCE=GGCTTCTTCGTAGGGTTCCT
PRIMER_LEFT_88=418,23
PRIMER_RIGHT_88=994,20
PRIMER_LEFT_88_TM=61.514
PRIMER_RIGHT_88_TM=59.713
PRIMER_LEFT_88_GC_PERCENT=52.174
PRIMER_RIGHT_88_GC_PERCENT=55.000
PRIMER_LEFT_88_SELF_ANY=4.00
PRIMER_RIGHT_88_SELF_ANY=4.00
PRIMER_LEFT_88_SELF_END=2.00
PRIMER_RIGHT_88_SELF_END=3.00
PRIMER_LEFT_88_END_STABILITY=7.9000
PRIMER_RIGHT_88_END_STABILITY=8.2000
PRIMER_PAIR_88_COMPL_ANY=6.00
PRIMER_PAIR_88_COMPL_END=3.00
PRIMER_PAIR_88_PRODUCT_SIZE=577
PRIMER_PAIR_88_PRODUCT_TM=80.1
PRIMER_PAIR_89_PENALTY=4.820376
PRIMER_LEFT_89_PENALTY=4.514227
PRIMER_RIGHT_89_PENALTY=0.306150
PRIMER_LEFT_89_SEQUENCE=GCCAAGAAGTCTACCCTGAACTG
PRIMER_RIGHT_89_SEQUENCE=AAAGTACCAGCGGGAGATCA
PRIMER_LEFT_89=417,23
PRIMER_RIGHT_89=1126,20
PRIMER_LEFT_89_TM=61.514
PRIMER_RIGHT_89_TM=59.694
PRIMER_LEFT_89_GC_PERCENT=52.174
PRIMER_RIGHT_89_GC_PERCENT=50.000
PRIMER_LEFT_89_SELF_ANY=4.00
PRIMER_RIGHT_89_SELF_ANY=4.00
PRIMER_LEFT_89_SELF_END=1.00
PRIMER_RIGHT_89_SELF_END=2.00
PRIMER_LEFT_89_END_STABILITY=6.7000
PRIMER_RIGHT_89_END_STABILITY=6.6000
PRIMER_PAIR_89_COMPL_ANY=5.00
PRIMER_PAIR_89_COMPL_END=3.00
PRIMER_PAIR_89_PRODUCT_SIZE=710
PRIMER_PAIR_89_PRODUCT_TM=80.8
PRIMER_PAIR_90_PENALTY=4.834837
PRIMER_LEFT_90_PENALTY=4.514227
PRIMER_RIGHT_90_PENALTY=0.320610
PRIMER_LEFT_90_SEQUENCE=TGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_90_SEQUENCE=CTTTGGCTTTCTGGAAATGG
PRIMER_LEFT_90=416,23
PRIMER_RIGHT_90=1385,20
PRIMER_LEFT_90_TM=61.514
PRIMER_RIGHT_90_TM=59.679
PRIMER_LEFT_90_GC_PERCENT=47.826
PRIMER_RIGHT_90_GC_PERCENT=45.000
PRIMER_LEFT_90_SELF_ANY=4.00
PRIMER_RIGHT_90_SELF_ANY=6.00
PRIMER_LEFT_90_SELF_END=1.00
PRIMER_RIGHT_90_SELF_END=2.00
PRIMER_LEFT_90_END_STABILITY=6.4000
PRIMER_RIGHT_90_END_STABILITY=8.4000
PRIMER_PAIR_90_COMPL_ANY=5.00
PRIMER_PAIR_90_COMPL_END=0.00
PRIMER_PAIR_90_PRODUCT_SIZE=970
PRIMER_PAIR_90_PRODUCT_TM=80.9
PRIMER_PAIR_91_PENALTY=4.877959
PRIMER_LEFT_91_PENALTY=1.557214
PRIMER_RIGHT_91_PENALTY=3.320745
PRIMER_LEFT_91_SEQUENCE=ACCCACTGATGGTAATGCTG
PRIMER_RIGHT_91_SEQUENCE=CTCACCAACTAAGCAGCGGTAG
PRIMER_LEFT_91=259,20
PRIMER_RIGHT_91=562,22
PRIMER_LEFT_91_TM=58.443
PRIMER_RIGHT_91_TM=61.321
PRIMER_LEFT_91_GC_PERCENT=50.000
PRIMER_RIGHT_91_GC_PERCENT=54.545
PRIMER_LEFT_91_SELF_ANY=3.00
PRIMER_RIGHT_91_SELF_ANY=3.00
PRIMER_LEFT_91_SELF_END=1.00
PRIMER_RIGHT_91_SELF_END=3.00
PRIMER_LEFT_91_END_STABILITY=8.5000
PRIMER_RIGHT_91_END_STABILITY=6.9000
PRIMER_PAIR_91_COMPL_ANY=4.00
PRIMER_PAIR_91_COMPL_END=3.00
PRIMER_PAIR_91_PRODUCT_SIZE=304
PRIMER_PAIR_91_PRODUCT_TM=79.4
PRIMER_PAIR_92_PENALTY=4.884799
PRIMER_LEFT_92_PENALTY=1.590836
PRIMER_RIGHT_92_PENALTY=3.293963
PRIMER_LEFT_92_SEQUENCE=CAGATCCATCAGGGACCAA
PRIMER_RIGHT_92_SEQUENCE=CAAACTCACCAACTAAGCAGC
PRIMER_LEFT_92=360,19
PRIMER_RIGHT_92=566,21
PRIMER_LEFT_92_TM=59.409
PRIMER_RIGHT_92_TM=57.706
PRIMER_LEFT_92_GC_PERCENT=52.632
PRIMER_RIGHT_92_GC_PERCENT=47.619
PRIMER_LEFT_92_SELF_ANY=5.00
PRIMER_RIGHT_92_SELF_ANY=3.00
PRIMER_LEFT_92_SELF_END=1.00
PRIMER_RIGHT_92_SELF_END=3.00
PRIMER_LEFT_92_END_STABILITY=8.2000
PRIMER_RIGHT_92_END_STABILITY=9.7000
PRIMER_PAIR_92_COMPL_ANY=2.00
PRIMER_PAIR_92_COMPL_END=0.00
PRIMER_PAIR_92_PRODUCT_SIZE=207
PRIMER_PAIR_92_PRODUCT_TM=78.4
PRIMER_PAIR_93_PENALTY=4.925851
PRIMER_LEFT_93_PENALTY=2.664693
PRIMER_RIGHT_93_PENALTY=2.261158
PRIMER_LEFT_93_SEQUENCE=CTCCGTCAGTTTCCTCGG
PRIMER_RIGHT_93_SEQUENCE=CAGTGGGTACCTCCAGCG
PRIMER_LEFT_93=46,18
PRIMER_RIGHT_93=266,18
PRIMER_LEFT_93_TM=59.335
PRIMER_RIGHT_93_TM=60.261
PRIMER_LEFT_93_GC_PERCENT=61.111
PRIMER_RIGHT_93_GC_PERCENT=66.667
PRIMER_LEFT_93_SELF_ANY=3.00
PRIMER_RIGHT_93_SELF_ANY=6.00
PRIMER_LEFT_93_SELF_END=2.00
PRIMER_RIGHT_93_SELF_END=2.00
PRIMER_LEFT_93_END_STABILITY=9.9000
PRIMER_RIGHT_93_END_STABILITY=10.2000
PRIMER_PAIR_93_COMPL_ANY=3.00
PRIMER_PAIR_93_COMPL_END=1.00
PRIMER_PAIR_93_PRODUCT_SIZE=221
PRIMER_PAIR_93_PRODUCT_TM=87.8
PRIMER_PAIR_94_PENALTY=4.933547
PRIMER_LEFT_94_PENALTY=1.604227
PRIMER_RIGHT_94_PENALTY=3.329319
PRIMER_LEFT_94_SEQUENCE=GGGATTCAGATCCATCAGGG
PRIMER_RIGHT_94_SEQUENCE=TCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_94=354,20
PRIMER_RIGHT_94=683,23
PRIMER_LEFT_94_TM=61.604
PRIMER_RIGHT_94_TM=59.671
PRIMER_LEFT_94_GC_PERCENT=55.000
PRIMER_RIGHT_94_GC_PERCENT=43.478
PRIMER_LEFT_94_SELF_ANY=5.00
PRIMER_RIGHT_94_SELF_ANY=4.00
PRIMER_LEFT_94_SELF_END=3.00
PRIMER_RIGHT_94_SELF_END=0.00
PRIMER_LEFT_94_END_STABILITY=9.7000
PRIMER_RIGHT_94_END_STABILITY=7.3000
PRIMER_PAIR_94_COMPL_ANY=5.00
PRIMER_PAIR_94_COMPL_END=2.00
PRIMER_PAIR_94_PRODUCT_SIZE=330
PRIMER_PAIR_94_PRODUCT_TM=78.8
PRIMER_PAIR_95_PENALTY=4.947233
PRIMER_LEFT_95_PENALTY=1.604487
PRIMER_RIGHT_95_PENALTY=3.342747
PRIMER_LEFT_95_SEQUENCE=AGGGACCAAAACCTGCATT
PRIMER_RIGHT_95_SEQUENCE=GTCTTCACTCCCATCTGCATAGT
PRIMER_LEFT_95=370,19
PRIMER_RIGHT_95=868,23
PRIMER_LEFT_95_TM=59.396
PRIMER_RIGHT_95_TM=59.657
PRIMER_LEFT_95_GC_PERCENT=47.368
PRIMER_RIGHT_95_GC_PERCENT=47.826
PRIMER_LEFT_95_SELF_ANY=4.00
PRIMER_RIGHT_95_SELF_ANY=4.00
PRIMER_LEFT_95_SELF_END=1.00
PRIMER_RIGHT_95_SELF_END=2.00
PRIMER_LEFT_95_END_STABILITY=8.4000
PRIMER_RIGHT_95_END_STABILITY=5.3000
PRIMER_PAIR_95_COMPL_ANY=4.00
PRIMER_PAIR_95_COMPL_END=1.00
PRIMER_PAIR_95_PRODUCT_SIZE=499
PRIMER_PAIR_95_PRODUCT_TM=79.9
PRIMER_PAIR_96_PENALTY=5.016847
PRIMER_LEFT_96_PENALTY=1.604487
PRIMER_RIGHT_96_PENALTY=3.412360
PRIMER_LEFT_96_SEQUENCE=GAATGTCCAGAATGGGAAGTG
PRIMER_RIGHT_96_SEQUENCE=GGCTGCTGTTGTAGGAAGTTTA
PRIMER_LEFT_96=334,21
PRIMER_RIGHT_96=1306,22
PRIMER_LEFT_96_TM=59.396
PRIMER_RIGHT_96_TM=58.588
PRIMER_LEFT_96_GC_PERCENT=47.619
PRIMER_RIGHT_96_GC_PERCENT=45.455
PRIMER_LEFT_96_SELF_ANY=3.00
PRIMER_RIGHT_96_SELF_ANY=3.00
PRIMER_LEFT_96_SELF_END=0.00
PRIMER_RIGHT_96_SELF_END=2.00
PRIMER_LEFT_96_END_STABILITY=6.7000
PRIMER_RIGHT_96_END_STABILITY=6.0000
PRIMER_PAIR_96_COMPL_ANY=3.00
PRIMER_PAIR_96_COMPL_END=0.00
PRIMER_PAIR_96_PRODUCT_SIZE=973
PRIMER_PAIR_96_PRODUCT_TM=80.8
PRIMER_PAIR_97_PENALTY=5.018264
PRIMER_LEFT_97_PENALTY=1.604487
PRIMER_RIGHT_97_PENALTY=3.413777
PRIMER_LEFT_97_SEQUENCE=TGAATGTCCAGAATGGGAAGT
PRIMER_RIGHT_97_SEQUENCE=ACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_97=333,21
PRIMER_RIGHT_97=567,22
PRIMER_LEFT_97_TM=59.396
PRIMER_RIGHT_97_TM=58.586
PRIMER_LEFT_97_GC_PERCENT=42.857
PRIMER_RIGHT_97_GC_PERCENT=45.455
PRIMER_LEFT_97_SELF_ANY=3.00
PRIMER_RIGHT_97_SELF_ANY=3.00
PRIMER_LEFT_97_SELF_END=1.00
PRIMER_RIGHT_97_SELF_END=3.00
PRIMER_LEFT_97_END_STABILITY=6.4000
PRIMER_RIGHT_97_END_STABILITY=9.7000
PRIMER_PAIR_97_COMPL_ANY=4.00
PRIMER_PAIR_97_COMPL_END=0.00
PRIMER_PAIR_97_PRODUCT_SIZE=235
PRIMER_PAIR_97_PRODUCT_TM=78.3
PRIMER_PAIR_98_PENALTY=5.114433
PRIMER_LEFT_98_PENALTY=1.628165
PRIMER_RIGHT_98_PENALTY=3.486268
PRIMER_LEFT_98_SEQUENCE=TCCAGAATGGGAAGTGGGAT
PRIMER_RIGHT_98_SEQUENCE=TCACCAACTAAGCAGCGG
PRIMER_LEFT_98=339,20
PRIMER_RIGHT_98=561,18
PRIMER_LEFT_98_TM=61.628
PRIMER_RIGHT_98_TM=58.514
PRIMER_LEFT_98_GC_PERCENT=50.000
PRIMER_RIGHT_98_GC_PERCENT=55.556
PRIMER_LEFT_98_SELF_ANY=3.00
PRIMER_RIGHT_98_SELF_ANY=3.00
PRIMER_LEFT_98_SELF_END=2.00
PRIMER_RIGHT_98_SELF_END=2.00
PRIMER_LEFT_98_END_STABILITY=9.3000
PRIMER_RIGHT_98_END_STABILITY=11.4000
PRIMER_PAIR_98_COMPL_ANY=4.00
PRIMER_PAIR_98_COMPL_END=0.00
PRIMER_PAIR_98_PRODUCT_SIZE=223
PRIMER_PAIR_98_PRODUCT_TM=78.5
PRIMER_PAIR_99_PENALTY=5.146917
PRIMER_LEFT_99_PENALTY=4.984244
PRIMER_RIGHT_99_PENALTY=0.162673
PRIMER_LEFT_99_SEQUENCE=GCTGGAGGTACCCACTGA
PRIMER_RIGHT_99_SEQUENCE=CTTGTCAGGAACGAGAAGGG
PRIMER_LEFT_99=250,18
PRIMER_RIGHT_99=595,20
PRIMER_LEFT_99_TM=57.016
PRIMER_RIGHT_99_TM=59.837
PRIMER_LEFT_99_GC_PERCENT=61.111
PRIMER_RIGHT_99_GC_PERCENT=55.000
PRIMER_LEFT_99_SELF_ANY=6.00
PRIMER_RIGHT_99_SELF_ANY=4.00
PRIMER_LEFT_99_SELF_END=2.00
PRIMER_RIGHT_99_SELF_END=0.00
PRIMER_LEFT_99_END_STABILITY=6.4000
PRIMER_RIGHT_99_END_STABILITY=9.7000
PRIMER_PAIR_99_COMPL_ANY=4.00
PRIMER_PAIR_99_COMPL_END=0.00
PRIMER_PAIR_99_PRODUCT_SIZE=346
PRIMER_PAIR_99_PRODUCT_TM=79.5
PRIMER_PAIR_100_PENALTY=5.153178
PRIMER_LEFT_100_PENALTY=4.847028
PRIMER_RIGHT_100_PENALTY=0.306150
PRIMER_LEFT_100_SEQUENCE=TTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_100_SEQUENCE=TCAAAGTACCAGCGGGAGAT
PRIMER_LEFT_100=415,23
PRIMER_RIGHT_100=1128,20
PRIMER_LEFT_100_TM=61.847
PRIMER_RIGHT_100_TM=59.694
PRIMER_LEFT_100_GC_PERCENT=47.826
PRIMER_RIGHT_100_GC_PERCENT=50.000
PRIMER_LEFT_100_SELF_ANY=5.00
PRIMER_RIGHT_100_SELF_ANY=4.00
PRIMER_LEFT_100_SELF_END=0.00
PRIMER_RIGHT_100_SELF_END=2.00
PRIMER_LEFT_100_END_STABILITY=6.7000
PRIMER_RIGHT_100_END_STABILITY=6.3000
PRIMER_PAIR_100_COMPL_ANY=5.00
PRIMER_PAIR_100_COMPL_END=3.00
PRIMER_PAIR_100_PRODUCT_SIZE=714
PRIMER_PAIR_100_PRODUCT_TM=80.8
PRIMER_PAIR_101_PENALTY=5.186407
PRIMER_LEFT_101_PENALTY=4.860451
PRIMER_RIGHT_101_PENALTY=0.325957
PRIMER_LEFT_101_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_101_SEQUENCE=CATTCTCATCCCCAGGTGTC
PRIMER_LEFT_101=412,24
PRIMER_RIGHT_101=1358,20
PRIMER_LEFT_101_TM=60.860
PRIMER_RIGHT_101_TM=60.326
PRIMER_LEFT_101_GC_PERCENT=45.833
PRIMER_RIGHT_101_GC_PERCENT=55.000
PRIMER_LEFT_101_SELF_ANY=5.00
PRIMER_RIGHT_101_SELF_ANY=3.00
PRIMER_LEFT_101_SELF_END=1.00
PRIMER_RIGHT_101_SELF_END=1.00
PRIMER_LEFT_101_END_STABILITY=8.2000
PRIMER_RIGHT_101_END_STABILITY=6.1000
PRIMER_PAIR_101_COMPL_ANY=4.00
PRIMER_PAIR_101_COMPL_END=2.00
PRIMER_PAIR_101_PRODUCT_SIZE=947
PRIMER_PAIR_101_PRODUCT_TM=80.9
PRIMER_PAIR_102_PENALTY=5.228019
PRIMER_LEFT_102_PENALTY=2.567306
PRIMER_RIGHT_102_PENALTY=2.660713
PRIMER_LEFT_102_SEQUENCE=GTTTGGCACTGCTCCTGC
PRIMER_RIGHT_102_SEQUENCE=GTTCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_102=210,18
PRIMER_RIGHT_102=437,22
PRIMER_LEFT_102_TM=60.567
PRIMER_RIGHT_102_TM=60.661
PRIMER_LEFT_102_GC_PERCENT=61.111
PRIMER_RIGHT_102_GC_PERCENT=50.000
PRIMER_LEFT_102_SELF_ANY=5.00
PRIMER_RIGHT_102_SELF_ANY=4.00
PRIMER_LEFT_102_SELF_END=2.00
PRIMER_RIGHT_102_SELF_END=3.00
PRIMER_LEFT_102_END_STABILITY=9.7000
PRIMER_RIGHT_102_END_STABILITY=10.0000
PRIMER_PAIR_102_COMPL_ANY=4.00
PRIMER_PAIR_102_COMPL_END=3.00
PRIMER_PAIR_102_PRODUCT_SIZE=228
PRIMER_PAIR_102_PRODUCT_TM=79.7
PRIMER_PAIR_103_PENALTY=5.234457
PRIMER_LEFT_103_PENALTY=1.608005
PRIMER_RIGHT_103_PENALTY=3.626453
PRIMER_LEFT_103_SEQUENCE=CCTGCAGTATTGCCAAGAAGT
PRIMER_RIGHT_103_SEQUENCE=CTTTGTCTTCACTCCCATCTG
PRIMER_LEFT_103=406,21
PRIMER_RIGHT_103=872,21
PRIMER_LEFT_103_TM=59.392
PRIMER_RIGHT_103_TM=57.374
PRIMER_LEFT_103_GC_PERCENT=47.619
PRIMER_RIGHT_103_GC_PERCENT=47.619
PRIMER_LEFT_103_SELF_ANY=6.00
PRIMER_RIGHT_103_SELF_ANY=2.00
PRIMER_LEFT_103_SELF_END=1.00
PRIMER_RIGHT_103_SELF_END=2.00
PRIMER_LEFT_103_END_STABILITY=6.4000
PRIMER_RIGHT_103_END_STABILITY=6.6000
PRIMER_PAIR_103_COMPL_ANY=4.00
PRIMER_PAIR_103_COMPL_END=2.00
PRIMER_PAIR_103_PRODUCT_SIZE=467
PRIMER_PAIR_103_PRODUCT_TM=79.9
PRIMER_PAIR_104_PENALTY=5.275172
PRIMER_LEFT_104_PENALTY=1.630431
PRIMER_RIGHT_104_PENALTY=3.644741
PRIMER_LEFT_104_SEQUENCE=TGGCAGACTGAACATGCAC
PRIMER_RIGHT_104_SEQUENCE=ACTTTGGGACATGGCGCT
PRIMER_LEFT_104=313,19
PRIMER_RIGHT_104=1243,18
PRIMER_LEFT_104_TM=59.370
PRIMER_RIGHT_104_TM=61.645
PRIMER_LEFT_104_GC_PERCENT=52.632
PRIMER_RIGHT_104_GC_PERCENT=55.556
PRIMER_LEFT_104_SELF_ANY=5.00
PRIMER_RIGHT_104_SELF_ANY=4.00
PRIMER_LEFT_104_SELF_END=2.00
PRIMER_RIGHT_104_SELF_END=2.00
PRIMER_LEFT_104_END_STABILITY=8.2000
PRIMER_RIGHT_104_END_STABILITY=11.4000
PRIMER_PAIR_104_COMPL_ANY=4.00
PRIMER_PAIR_104_COMPL_END=1.00
PRIMER_PAIR_104_PRODUCT_SIZE=931
PRIMER_PAIR_104_PRODUCT_TM=80.8
PRIMER_PAIR_105_PENALTY=5.295677
PRIMER_LEFT_105_PENALTY=2.882010
PRIMER_RIGHT_105_PENALTY=2.413667
PRIMER_LEFT_105_SEQUENCE=CTGACTCGCCTGGCTCTG
PRIMER_RIGHT_105_SEQUENCE=ATCAGTGGGTACCTCCAGCG
PRIMER_LEFT_105=7,18
PRIMER_RIGHT_105=268,20
PRIMER_LEFT_105_TM=60.882
PRIMER_RIGHT_105_TM=62.414
PRIMER_LEFT_105_GC_PERCENT=66.667
PRIMER_RIGHT_105_GC_PERCENT=60.000
PRIMER_LEFT_105_SELF_ANY=5.00
PRIMER_RIGHT_105_SELF_ANY=6.00
PRIMER_LEFT_105_SELF_END=1.00
PRIMER_RIGHT_105_SELF_END=2.00
PRIMER_LEFT_105_END_STABILITY=6.7000
PRIMER_RIGHT_105_END_STABILITY=10.2000
PRIMER_PAIR_105_COMPL_ANY=4.00
PRIMER_PAIR_105_COMPL_END=3.00
PRIMER_PAIR_105_PRODUCT_SIZE=262
PRIMER_PAIR_105_PRODUCT_TM=88.3
PRIMER_PAIR_106_PENALTY=5.322012
PRIMER_LEFT_106_PENALTY=1.630431
PRIMER_RIGHT_106_PENALTY=3.691582
PRIMER_LEFT_106_SEQUENCE=TGTGGCAGACTGAACATGC
PRIMER_RIGHT_106_SEQUENCE=ACTTTGTCTTCACTCCCATCTG
PRIMER_LEFT_106=311,19
PRIMER_RIGHT_106=873,22
PRIMER_LEFT_106_TM=59.370
PRIMER_RIGHT_106_TM=58.308
PRIMER_LEFT_106_GC_PERCENT=52.632
PRIMER_RIGHT_106_GC_PERCENT=45.455
PRIMER_LEFT_106_SELF_ANY=5.00
PRIMER_RIGHT_106_SELF_ANY=2.00
PRIMER_LEFT_106_SELF_END=2.00
PRIMER_RIGHT_106_SELF_END=2.00
PRIMER_LEFT_106_END_STABILITY=8.4000
PRIMER_RIGHT_106_END_STABILITY=6.6000
PRIMER_PAIR_106_COMPL_ANY=5.00
PRIMER_PAIR_106_COMPL_END=1.00
PRIMER_PAIR_106_PRODUCT_SIZE=563
PRIMER_PAIR_106_PRODUCT_TM=79.7
PRIMER_PAIR_107_PENALTY=5.359258
PRIMER_LEFT_107_PENALTY=1.648882
PRIMER_RIGHT_107_PENALTY=3.710377
PRIMER_LEFT_107_SEQUENCE=AAGTGGGATTCAGATCCATCA
PRIMER_RIGHT_107_SEQUENCE=ACTGCATGTCTCTTTGGCGAC
PRIMER_LEFT_107=350,21
PRIMER_RIGHT_107=676,21
PRIMER_LEFT_107_TM=59.351
PRIMER_RIGHT_107_TM=62.710
PRIMER_LEFT_107_GC_PERCENT=42.857
PRIMER_RIGHT_107_GC_PERCENT=52.381
PRIMER_LEFT_107_SELF_ANY=5.00
PRIMER_RIGHT_107_SELF_ANY=4.00
PRIMER_LEFT_107_SELF_END=2.00
PRIMER_RIGHT_107_SELF_END=2.00
PRIMER_LEFT_107_END_STABILITY=6.9000
PRIMER_RIGHT_107_END_STABILITY=9.6000
PRIMER_PAIR_107_COMPL_ANY=5.00
PRIMER_PAIR_107_COMPL_END=0.00
PRIMER_PAIR_107_PRODUCT_SIZE=327
PRIMER_PAIR_107_PRODUCT_TM=78.8
PRIMER_PAIR_108_PENALTY=5.379232
PRIMER_LEFT_108_PENALTY=2.710504
PRIMER_RIGHT_108_PENALTY=2.668729
PRIMER_LEFT_108_SEQUENCE=TTTGGCACTGCTCCTGCT
PRIMER_RIGHT_108_SEQUENCE=CAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_108=211,18
PRIMER_RIGHT_108=434,21
PRIMER_LEFT_108_TM=60.711
PRIMER_RIGHT_108_TM=58.331
PRIMER_LEFT_108_GC_PERCENT=55.556
PRIMER_RIGHT_108_GC_PERCENT=47.619
PRIMER_LEFT_108_SELF_ANY=5.00
PRIMER_RIGHT_108_SELF_ANY=5.00
PRIMER_LEFT_108_SELF_END=0.00
PRIMER_RIGHT_108_SELF_END=3.00
PRIMER_LEFT_108_END_STABILITY=8.2000
PRIMER_RIGHT_108_END_STABILITY=8.4000
PRIMER_PAIR_108_COMPL_ANY=4.00
PRIMER_PAIR_108_COMPL_END=0.00
PRIMER_PAIR_108_PRODUCT_SIZE=224
PRIMER_PAIR_108_PRODUCT_TM=79.7
PRIMER_PAIR_109_PENALTY=5.478782
PRIMER_LEFT_109_PENALTY=1.651669
PRIMER_RIGHT_109_PENALTY=3.827113
PRIMER_LEFT_109_SEQUENCE=TCCATCAGGGACCAAAACCT
PRIMER_RIGHT_109_SEQUENCE=TGTCTTCACTCCCATCTGC
PRIMER_LEFT_109=364,20
PRIMER_RIGHT_109=869,19
PRIMER_LEFT_109_TM=61.652
PRIMER_RIGHT_109_TM=57.173
PRIMER_LEFT_109_GC_PERCENT=50.000
PRIMER_RIGHT_109_GC_PERCENT=52.632
PRIMER_LEFT_109_SELF_ANY=5.00
PRIMER_RIGHT_109_SELF_ANY=2.00
PRIMER_LEFT_109_SELF_END=0.00
PRIMER_RIGHT_109_SELF_END=2.00
PRIMER_LEFT_109_END_STABILITY=7.9000
PRIMER_RIGHT_109_END_STABILITY=8.2000
PRIMER_PAIR_109_COMPL_ANY=4.00
PRIMER_PAIR_109_COMPL_END=0.00
PRIMER_PAIR_109_PRODUCT_SIZE=506
PRIMER_PAIR_109_PRODUCT_TM=79.9
PRIMER_PAIR_110_PENALTY=5.566446
PRIMER_LEFT_110_PENALTY=1.659162
PRIMER_RIGHT_110_PENALTY=3.907284
PRIMER_LEFT_110_SEQUENCE=CATGTTCTGTGGCAGACTGA
PRIMER_RIGHT_110_SEQUENCE=TCAGAGCACACCTCTCGAACC
PRIMER_LEFT_110=304,20
PRIMER_RIGHT_110=1077,21
PRIMER_LEFT_110_TM=58.341
PRIMER_RIGHT_110_TM=62.907
PRIMER_LEFT_110_GC_PERCENT=50.000
PRIMER_RIGHT_110_GC_PERCENT=57.143
PRIMER_LEFT_110_SELF_ANY=5.00
PRIMER_RIGHT_110_SELF_ANY=4.00
PRIMER_LEFT_110_SELF_END=3.00
PRIMER_RIGHT_110_SELF_END=0.00
PRIMER_LEFT_110_END_STABILITY=6.4000
PRIMER_RIGHT_110_END_STABILITY=7.9000
PRIMER_PAIR_110_COMPL_ANY=5.00
PRIMER_PAIR_110_COMPL_END=0.00
PRIMER_PAIR_110_PRODUCT_SIZE=774
PRIMER_PAIR_110_PRODUCT_TM=80.3
PRIMER_PAIR_111_PENALTY=5.584852
PRIMER_LEFT_111_PENALTY=1.675600
PRIMER_RIGHT_111_PENALTY=3.909253
PRIMER_LEFT_111_SEQUENCE=TGGAGGTACCCACTGATGG
PRIMER_RIGHT_111_SEQUENCE=AACTTTGGGACATGGCGCT
PRIMER_LEFT_111=252,19
PRIMER_RIGHT_111=1244,19
PRIMER_LEFT_111_TM=59.324
PRIMER_RIGHT_111_TM=62.909
PRIMER_LEFT_111_GC_PERCENT=57.895
PRIMER_RIGHT_111_GC_PERCENT=52.632
PRIMER_LEFT_111_SELF_ANY=6.00
PRIMER_RIGHT_111_SELF_ANY=4.00
PRIMER_LEFT_111_SELF_END=3.00
PRIMER_RIGHT_111_SELF_END=2.00
PRIMER_LEFT_111_END_STABILITY=8.1000
PRIMER_RIGHT_111_END_STABILITY=11.4000
PRIMER_PAIR_111_COMPL_ANY=4.00
PRIMER_PAIR_111_COMPL_END=0.00
PRIMER_PAIR_111_PRODUCT_SIZE=993
PRIMER_PAIR_111_PRODUCT_TM=80.9
PRIMER_PAIR_112_PENALTY=5.597872
PRIMER_LEFT_112_PENALTY=5.291723
PRIMER_RIGHT_112_PENALTY=0.306150
PRIMER_LEFT_112_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_112_SEQUENCE=ATCAAAGTACCAGCGGGAGA
PRIMER_LEFT_112=413,24
PRIMER_RIGHT_112=1129,20
PRIMER_LEFT_112_TM=61.292
PRIMER_RIGHT_112_TM=59.694
PRIMER_LEFT_112_GC_PERCENT=41.667
PRIMER_RIGHT_112_GC_PERCENT=50.000
PRIMER_LEFT_112_SELF_ANY=5.00
PRIMER_RIGHT_112_SELF_ANY=4.00
PRIMER_LEFT_112_SELF_END=0.00
PRIMER_RIGHT_112_SELF_END=0.00
PRIMER_LEFT_112_END_STABILITY=7.0000
PRIMER_RIGHT_112_END_STABILITY=7.9000
PRIMER_PAIR_112_COMPL_ANY=5.00
PRIMER_PAIR_112_COMPL_END=3.00
PRIMER_PAIR_112_PRODUCT_SIZE=717
PRIMER_PAIR_112_PRODUCT_TM=80.7
PRIMER_PAIR_113_PENALTY=5.649800
PRIMER_LEFT_113_PENALTY=1.676675
PRIMER_RIGHT_113_PENALTY=3.973125
PRIMER_LEFT_113_SEQUENCE=GGATTCAGATCCATCAGGGAC
PRIMER_RIGHT_113_SEQUENCE=TTGTCTTCACTCCCATCTGCAT
PRIMER_LEFT_113=355,21
PRIMER_RIGHT_113=870,22
PRIMER_LEFT_113_TM=60.677
PRIMER_RIGHT_113_TM=61.973
PRIMER_LEFT_113_GC_PERCENT=52.381
PRIMER_RIGHT_113_GC_PERCENT=45.455
PRIMER_LEFT_113_SELF_ANY=5.00
PRIMER_RIGHT_113_SELF_ANY=4.00
PRIMER_LEFT_113_SELF_END=3.00
PRIMER_RIGHT_113_SELF_END=2.00
PRIMER_LEFT_113_END_STABILITY=9.1000
PRIMER_RIGHT_113_END_STABILITY=8.4000
PRIMER_PAIR_113_COMPL_ANY=6.00
PRIMER_PAIR_113_COMPL_END=3.00
PRIMER_PAIR_113_PRODUCT_SIZE=516
PRIMER_PAIR_113_PRODUCT_TM=79.8
PRIMER_PAIR_114_PENALTY=5.735954
PRIMER_LEFT_114_PENALTY=5.409997
PRIMER_RIGHT_114_PENALTY=0.325957
PRIMER_LEFT_114_SEQUENCE=AGTATTGCCAAGAAGTCTACCCT
PRIMER_RIGHT_114_SEQUENCE=TCATTCTCATCCCCAGGTGT
PRIMER_LEFT_114=411,23
PRIMER_RIGHT_114=1359,20
PRIMER_LEFT_114_TM=57.590
PRIMER_RIGHT_114_TM=60.326
PRIMER_LEFT_114_GC_PERCENT=43.478
PRIMER_RIGHT_114_GC_PERCENT=50.000
PRIMER_LEFT_114_SELF_ANY=5.00
PRIMER_RIGHT_114_SELF_ANY=3.00
PRIMER_LEFT_114_SELF_END=2.00
PRIMER_RIGHT_114_SELF_END=1.00
PRIMER_LEFT_114_END_STABILITY=9.1000
PRIMER_RIGHT_114_END_STABILITY=7.6000
PRIMER_PAIR_114_COMPL_ANY=4.00
PRIMER_PAIR_114_COMPL_END=3.00
PRIMER_PAIR_114_PRODUCT_SIZE=949
PRIMER_PAIR_114_PRODUCT_TM=80.9
PRIMER_PAIR_115_PENALTY=5.795712
PRIMER_LEFT_115_PENALTY=1.683804
PRIMER_RIGHT_115_PENALTY=4.111908
PRIMER_LEFT_115_SEQUENCE=ACCCCAGATTGCCATGTTCT
PRIMER_RIGHT_115_SEQUENCE=TTACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_115=292,20
PRIMER_RIGHT_115=569,24
PRIMER_LEFT_115_TM=61.684
PRIMER_RIGHT_115_TM=59.888
PRIMER_LEFT_115_GC_PERCENT=50.000
PRIMER_RIGHT_115_GC_PERCENT=41.667
PRIMER_LEFT_115_SELF_ANY=4.00
PRIMER_RIGHT_115_SELF_ANY=3.00
PRIMER_LEFT_115_SELF_END=0.00
PRIMER_RIGHT_115_SELF_END=3.00
PRIMER_LEFT_115_END_STABILITY=6.4000
PRIMER_RIGHT_115_END_STABILITY=9.7000
PRIMER_PAIR_115_COMPL_ANY=4.00
PRIMER_PAIR_115_COMPL_END=1.00
PRIMER_PAIR_115_PRODUCT_SIZE=278
PRIMER_PAIR_115_PRODUCT_TM=78.5
PRIMER_PAIR_116_PENALTY=5.819620
PRIMER_LEFT_116_PENALTY=1.707711
PRIMER_RIGHT_116_PENALTY=4.111908
PRIMER_LEFT_116_SEQUENCE=CATGAATGTCCAGAATGGGAA
PRIMER_RIGHT_116_SEQUENCE=GCTGCTGTTGTAGGAAGTTTAACA
PRIMER_LEFT_116=331,21
PRIMER_RIGHT_116=1305,24
PRIMER_LEFT_116_TM=60.708
PRIMER_RIGHT_116_TM=59.888
PRIMER_LEFT_116_GC_PERCENT=42.857
PRIMER_RIGHT_116_GC_PERCENT=41.667
PRIMER_LEFT_116_SELF_ANY=4.00
PRIMER_RIGHT_116_SELF_ANY=5.00
PRIMER_LEFT_116_SELF_END=1.00
PRIMER_RIGHT_116_SELF_END=3.00
PRIMER_LEFT_116_END_STABILITY=9.7000
PRIMER_RIGHT_116_END_STABILITY=6.0000
PRIMER_PAIR_116_COMPL_ANY=3.00
PRIMER_PAIR_116_COMPL_END=2.00
PRIMER_PAIR_116_PRODUCT_SIZE=975
PRIMER_PAIR_116_PRODUCT_TM=80.8
PRIMER_PAIR_117_PENALTY=5.865112
PRIMER_LEFT_117_PENALTY=1.709051
PRIMER_RIGHT_117_PENALTY=4.156061
PRIMER_LEFT_117_SEQUENCE=TCCATCAGGGACCAAAACC
PRIMER_RIGHT_117_SEQUENCE=TGGCTGCTGTTGTAGGAAGTTTA
PRIMER_LEFT_117=364,19
PRIMER_RIGHT_117=1307,23
PRIMER_LEFT_117_TM=60.709
PRIMER_RIGHT_117_TM=61.156
PRIMER_LEFT_117_GC_PERCENT=52.632
PRIMER_RIGHT_117_GC_PERCENT=43.478
PRIMER_LEFT_117_SELF_ANY=5.00
PRIMER_RIGHT_117_SELF_ANY=3.00
PRIMER_LEFT_117_SELF_END=0.00
PRIMER_RIGHT_117_SELF_END=2.00
PRIMER_LEFT_117_END_STABILITY=8.2000
PRIMER_RIGHT_117_END_STABILITY=6.0000
PRIMER_PAIR_117_COMPL_ANY=4.00
PRIMER_PAIR_117_COMPL_END=2.00
PRIMER_PAIR_117_PRODUCT_SIZE=944
PRIMER_PAIR_117_PRODUCT_TM=80.9
PRIMER_PAIR_118_PENALTY=5.939864
PRIMER_LEFT_118_PENALTY=1.741437
PRIMER_RIGHT_118_PENALTY=4.198427
PRIMER_LEFT_118_SEQUENCE=ACCCCAGATTGCCATGTTC
PRIMER_RIGHT_118_SEQUENCE=CAAACTCACCAACTAAGCAGCG
PRIMER_LEFT_118=292,19
PRIMER_RIGHT_118=566,22
PRIMER_LEFT_118_TM=60.741
PRIMER_RIGHT_118_TM=62.198
PRIMER_LEFT_118_GC_PERCENT=52.632
PRIMER_RIGHT_118_GC_PERCENT=50.000
PRIMER_LEFT_118_SELF_ANY=4.00
PRIMER_RIGHT_118_SELF_ANY=3.00
PRIMER_LEFT_118_SELF_END=0.00
PRIMER_RIGHT_118_SELF_END=2.00
PRIMER_LEFT_118_END_STABILITY=6.7000
PRIMER_RIGHT_118_END_STABILITY=10.2000
PRIMER_PAIR_118_COMPL_ANY=4.00
PRIMER_PAIR_118_COMPL_END=1.00
PRIMER_PAIR_118_PRODUCT_SIZE=275
PRIMER_PAIR_118_PRODUCT_TM=78.7
PRIMER_PAIR_119_PENALTY=5.966723
PRIMER_LEFT_119_PENALTY=1.757583
PRIMER_RIGHT_119_PENALTY=4.209140
PRIMER_LEFT_119_SEQUENCE=ATGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_119_SEQUENCE=AGCACACCTCTCGAACCA
PRIMER_LEFT_119=345,20
PRIMER_RIGHT_119=1073,18
PRIMER_LEFT_119_TM=58.242
PRIMER_RIGHT_119_TM=57.791
PRIMER_LEFT_119_GC_PERCENT=45.000
PRIMER_RIGHT_119_GC_PERCENT=55.556
PRIMER_LEFT_119_SELF_ANY=3.00
PRIMER_RIGHT_119_SELF_ANY=4.00
PRIMER_LEFT_119_SELF_END=2.00
PRIMER_RIGHT_119_SELF_END=0.00
PRIMER_LEFT_119_END_STABILITY=6.6000
PRIMER_RIGHT_119_END_STABILITY=8.2000
PRIMER_PAIR_119_COMPL_ANY=4.00
PRIMER_PAIR_119_COMPL_END=2.00
PRIMER_PAIR_119_PRODUCT_SIZE=729
PRIMER_PAIR_119_PRODUCT_TM=80.4
PRIMER_PAIR_120_PENALTY=6.039199
PRIMER_LEFT_120_PENALTY=1.798843
PRIMER_RIGHT_120_PENALTY=4.240356
PRIMER_LEFT_120_SEQUENCE=ACATGAATGTCCAGAATGGG
PRIMER_RIGHT_120_SEQUENCE=CTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_120=330,20
PRIMER_RIGHT_120=682,22
PRIMER_LEFT_120_TM=58.201
PRIMER_RIGHT_120_TM=57.760
PRIMER_LEFT_120_GC_PERCENT=45.000
PRIMER_RIGHT_120_GC_PERCENT=45.455
PRIMER_LEFT_120_SELF_ANY=6.00
PRIMER_RIGHT_120_SELF_ANY=4.00
PRIMER_LEFT_120_SELF_END=1.00
PRIMER_RIGHT_120_SELF_END=0.00
PRIMER_LEFT_120_END_STABILITY=9.6000
PRIMER_RIGHT_120_END_STABILITY=7.3000
PRIMER_PAIR_120_COMPL_ANY=6.00
PRIMER_PAIR_120_COMPL_END=1.00
PRIMER_PAIR_120_PRODUCT_SIZE=353
PRIMER_PAIR_120_PRODUCT_TM=78.8
PRIMER_PAIR_121_PENALTY=6.052391
PRIMER_LEFT_121_PENALTY=2.974589
PRIMER_RIGHT_121_PENALTY=3.077802
PRIMER_LEFT_121_SEQUENCE=GGATCAGCTGACTCGCCT
PRIMER_RIGHT_121_SEQUENCE=GTTCAGGGTAGACTTCTTGGC
PRIMER_LEFT_121=0,18
PRIMER_RIGHT_121=437,21
PRIMER_LEFT_121_TM=59.025
PRIMER_RIGHT_121_TM=57.922
PRIMER_LEFT_121_GC_PERCENT=61.111
PRIMER_RIGHT_121_GC_PERCENT=52.381
PRIMER_LEFT_121_SELF_ANY=8.00
PRIMER_RIGHT_121_SELF_ANY=4.00
PRIMER_LEFT_121_SELF_END=3.00
PRIMER_RIGHT_121_SELF_END=2.00
PRIMER_LEFT_121_END_STABILITY=11.4000
PRIMER_RIGHT_121_END_STABILITY=10.0000
PRIMER_PAIR_121_COMPL_ANY=4.00
PRIMER_PAIR_121_COMPL_END=2.00
PRIMER_PAIR_121_PRODUCT_SIZE=438
PRIMER_PAIR_121_PRODUCT_TM=85.2
PRIMER_PAIR_122_PENALTY=6.106046
PRIMER_LEFT_122_PENALTY=3.028072
PRIMER_RIGHT_122_PENALTY=3.077974
PRIMER_LEFT_122_SEQUENCE=ATCCCACTCGCACAGCAG
PRIMER_RIGHT_122_SEQUENCE=AGTTCAGGGTAGACTTCTTGGC
PRIMER_LEFT_122=154,18
PRIMER_RIGHT_122=438,22
PRIMER_LEFT_122_TM=61.028
PRIMER_RIGHT_122_TM=58.922
PRIMER_LEFT_122_GC_PERCENT=61.111
PRIMER_RIGHT_122_GC_PERCENT=50.000
PRIMER_LEFT_122_SELF_ANY=2.00
PRIMER_RIGHT_122_SELF_ANY=4.00
PRIMER_LEFT_122_SELF_END=2.00
PRIMER_RIGHT_122_SELF_END=2.00
PRIMER_LEFT_122_END_STABILITY=8.2000
PRIMER_RIGHT_122_END_STABILITY=10.0000
PRIMER_PAIR_122_COMPL_ANY=3.00
PRIMER_PAIR_122_COMPL_END=3.00
PRIMER_PAIR_122_PRODUCT_SIZE=285
PRIMER_PAIR_122_PRODUCT_TM=81.7
PRIMER_PAIR_123_PENALTY=6.149183
PRIMER_LEFT_123_PENALTY=1.907724
PRIMER_RIGHT_123_PENALTY=4.241458
PRIMER_LEFT_123_SEQUENCE=CAGGGACCAAAACCTGCAT
PRIMER_RIGHT_123_SEQUENCE=TGCTGTTGTAGGAAGTTTAACAGG
PRIMER_LEFT_123=369,19
PRIMER_RIGHT_123=1303,24
PRIMER_LEFT_123_TM=60.908
PRIMER_RIGHT_123_TM=59.759
PRIMER_LEFT_123_GC_PERCENT=52.632
PRIMER_RIGHT_123_GC_PERCENT=41.667
PRIMER_LEFT_123_SELF_ANY=4.00
PRIMER_RIGHT_123_SELF_ANY=5.00
PRIMER_LEFT_123_SELF_END=2.00
PRIMER_RIGHT_123_SELF_END=1.00
PRIMER_LEFT_123_END_STABILITY=8.4000
PRIMER_RIGHT_123_END_STABILITY=7.9000
PRIMER_PAIR_123_COMPL_ANY=4.00
PRIMER_PAIR_123_COMPL_END=2.00
PRIMER_PAIR_123_PRODUCT_SIZE=935
PRIMER_PAIR_123_PRODUCT_TM=80.8
PRIMER_PAIR_124_PENALTY=6.152874
PRIMER_LEFT_124_PENALTY=1.811785
PRIMER_RIGHT_124_PENALTY=4.341090
PRIMER_LEFT_124_SEQUENCE=GGTACCCACTGATGGTAATGC
PRIMER_RIGHT_124_SEQUENCE=CTTCTCACTGCATGTCTCTTTGG
PRIMER_LEFT_124=256,21
PRIMER_RIGHT_124=682,23
PRIMER_LEFT_124_TM=59.188
PRIMER_RIGHT_124_TM=61.341
PRIMER_LEFT_124_GC_PERCENT=52.381
PRIMER_RIGHT_124_GC_PERCENT=47.826
PRIMER_LEFT_124_SELF_ANY=6.00
PRIMER_RIGHT_124_SELF_ANY=4.00
PRIMER_LEFT_124_SELF_END=2.00
PRIMER_RIGHT_124_SELF_END=0.00
PRIMER_LEFT_124_END_STABILITY=8.4000
PRIMER_RIGHT_124_END_STABILITY=8.8000
PRIMER_PAIR_124_COMPL_ANY=4.00
PRIMER_PAIR_124_COMPL_END=2.00
PRIMER_PAIR_124_PRODUCT_SIZE=427
PRIMER_PAIR_124_PRODUCT_TM=79.4
PRIMER_PAIR_125_PENALTY=6.157949
PRIMER_LEFT_125_PENALTY=5.821907
PRIMER_RIGHT_125_PENALTY=0.336042
PRIMER_LEFT_125_SEQUENCE=TGCAGTATTGCCAAGAAGTCTACC
PRIMER_RIGHT_125_SEQUENCE=GGTTCCTCAGCCTCTTCCTC
PRIMER_LEFT_125=408,24
PRIMER_RIGHT_125=981,20
PRIMER_LEFT_125_TM=61.822
PRIMER_RIGHT_125_TM=60.336
PRIMER_LEFT_125_GC_PERCENT=45.833
PRIMER_RIGHT_125_GC_PERCENT=60.000
PRIMER_LEFT_125_SELF_ANY=6.00
PRIMER_RIGHT_125_SELF_ANY=3.00
PRIMER_LEFT_125_SELF_END=1.00
PRIMER_RIGHT_125_SELF_END=0.00
PRIMER_LEFT_125_END_STABILITY=6.9000
PRIMER_RIGHT_125_END_STABILITY=7.9000
PRIMER_PAIR_125_COMPL_ANY=4.00
PRIMER_PAIR_125_COMPL_END=0.00
PRIMER_PAIR_125_PRODUCT_SIZE=574
PRIMER_PAIR_125_PRODUCT_TM=80.0
PRIMER_PAIR_126_PENALTY=6.229736
PRIMER_LEFT_126_PENALTY=1.876405
PRIMER_RIGHT_126_PENALTY=4.353331
PRIMER_LEFT_126_SEQUENCE=CCCCAGATTGCCATGTTCT
PRIMER_RIGHT_126_SEQUENCE=TACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_126=293,19
PRIMER_RIGHT_126=568,23
PRIMER_LEFT_126_TM=60.876
PRIMER_RIGHT_126_TM=58.647
PRIMER_LEFT_126_GC_PERCENT=52.632
PRIMER_RIGHT_126_GC_PERCENT=43.478
PRIMER_LEFT_126_SELF_ANY=4.00
PRIMER_RIGHT_126_SELF_ANY=3.00
PRIMER_LEFT_126_SELF_END=0.00
PRIMER_RIGHT_126_SELF_END=3.00
PRIMER_LEFT_126_END_STABILITY=6.4000
PRIMER_RIGHT_126_END_STABILITY=9.7000
PRIMER_PAIR_126_COMPL_ANY=4.00
PRIMER_PAIR_126_COMPL_END=1.00
PRIMER_PAIR_126_PRODUCT_SIZE=276
PRIMER_PAIR_126_PRODUCT_TM=78.7
PRIMER_PAIR_127_PENALTY=6.284438
PRIMER_LEFT_127_PENALTY=5.925828
PRIMER_RIGHT_127_PENALTY=0.358609
PRIMER_LEFT_127_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTA
PRIMER_RIGHT_127_SEQUENCE=CAGTGGGCAACACACAAACT
PRIMER_LEFT_127=406,24
PRIMER_RIGHT_127=766,20
PRIMER_LEFT_127_TM=61.926
PRIMER_RIGHT_127_TM=59.641
PRIMER_LEFT_127_GC_PERCENT=45.833
PRIMER_RIGHT_127_GC_PERCENT=50.000
PRIMER_LEFT_127_SELF_ANY=6.00
PRIMER_RIGHT_127_SELF_ANY=3.00
PRIMER_LEFT_127_SELF_END=2.00
PRIMER_RIGHT_127_SELF_END=1.00
PRIMER_LEFT_127_END_STABILITY=5.4000
PRIMER_RIGHT_127_END_STABILITY=6.7000
PRIMER_PAIR_127_COMPL_ANY=6.00
PRIMER_PAIR_127_COMPL_END=2.00
PRIMER_PAIR_127_PRODUCT_SIZE=361
PRIMER_PAIR_127_PRODUCT_TM=79.4
PRIMER_PAIR_128_PENALTY=6.362197
PRIMER_LEFT_128_PENALTY=6.000430
PRIMER_RIGHT_128_PENALTY=0.361767
PRIMER_LEFT_128_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_128_SEQUENCE=CGTCATCATCGGCTTCTTCT
PRIMER_LEFT_128=414,24
PRIMER_RIGHT_128=935,20
PRIMER_LEFT_128_TM=62.000
PRIMER_RIGHT_128_TM=60.362
PRIMER_LEFT_128_GC_PERCENT=45.833
PRIMER_RIGHT_128_GC_PERCENT=50.000
PRIMER_LEFT_128_SELF_ANY=5.00
PRIMER_RIGHT_128_SELF_ANY=3.00
PRIMER_LEFT_128_SELF_END=0.00
PRIMER_RIGHT_128_SELF_END=0.00
PRIMER_LEFT_128_END_STABILITY=6.7000
PRIMER_RIGHT_128_END_STABILITY=6.7000
PRIMER_PAIR_128_COMPL_ANY=6.00
PRIMER_PAIR_128_COMPL_END=3.00
PRIMER_PAIR_128_PRODUCT_SIZE=522
PRIMER_PAIR_128_PRODUCT_TM=79.8
PRIMER_PAIR_129_PENALTY=6.380664
PRIMER_LEFT_129_PENALTY=3.124765
PRIMER_RIGHT_129_PENALTY=3.255899
PRIMER_LEFT_129_SEQUENCE=GGGCTCCGTCAGTTTCCTC
PRIMER_RIGHT_129_SEQUENCE=TCAGTGGGTACCTCCAGCG
PRIMER_LEFT_129=43,19
PRIMER_RIGHT_129=267,19
PRIMER_LEFT_129_TM=62.125
PRIMER_RIGHT_129_TM=62.256
PRIMER_LEFT_129_GC_PERCENT=63.158
PRIMER_RIGHT_129_GC_PERCENT=63.158
PRIMER_LEFT_129_SELF_ANY=2.00
PRIMER_RIGHT_129_SELF_ANY=6.00
PRIMER_LEFT_129_SELF_END=0.00
PRIMER_RIGHT_129_SELF_END=2.00
PRIMER_LEFT_129_END_STABILITY=7.9000
PRIMER_RIGHT_129_END_STABILITY=10.2000
PRIMER_PAIR_129_COMPL_ANY=3.00
PRIMER_PAIR_129_COMPL_END=1.00
PRIMER_PAIR_129_PRODUCT_SIZE=225
PRIMER_PAIR_129_PRODUCT_TM=87.8
PRIMER_PAIR_130_PENALTY=6.439739
PRIMER_LEFT_130_PENALTY=2.051254
PRIMER_RIGHT_130_PENALTY=4.388485
PRIMER_LEFT_130_SEQUENCE=AACCCCAGATTGCCATGTTC
PRIMER_RIGHT_130_SEQUENCE=GCACACCTCTCGAACCAC
PRIMER_LEFT_130=291,20
PRIMER_RIGHT_130=1072,18
PRIMER_LEFT_130_TM=62.051
PRIMER_RIGHT_130_TM=57.612
PRIMER_LEFT_130_GC_PERCENT=50.000
PRIMER_RIGHT_130_GC_PERCENT=61.111
PRIMER_LEFT_130_SELF_ANY=4.00
PRIMER_RIGHT_130_SELF_ANY=4.00
PRIMER_LEFT_130_SELF_END=0.00
PRIMER_RIGHT_130_SELF_END=0.00
PRIMER_LEFT_130_END_STABILITY=6.7000
PRIMER_RIGHT_130_END_STABILITY=7.6000
PRIMER_PAIR_130_COMPL_ANY=4.00
PRIMER_PAIR_130_COMPL_END=2.00
PRIMER_PAIR_130_PRODUCT_SIZE=782
PRIMER_PAIR_130_PRODUCT_TM=80.4
PRIMER_PAIR_131_PENALTY=6.463179
PRIMER_LEFT_131_PENALTY=2.051254
PRIMER_RIGHT_131_PENALTY=4.411925
PRIMER_LEFT_131_SEQUENCE=GAACCCCAGATTGCCATGTT
PRIMER_RIGHT_131_SEQUENCE=AACTCACCAACTAAGCAGCGGT
PRIMER_LEFT_131=290,20
PRIMER_RIGHT_131=564,22
PRIMER_LEFT_131_TM=62.051
PRIMER_RIGHT_131_TM=62.412
PRIMER_LEFT_131_GC_PERCENT=50.000
PRIMER_RIGHT_131_GC_PERCENT=50.000
PRIMER_LEFT_131_SELF_ANY=4.00
PRIMER_RIGHT_131_SELF_ANY=3.00
PRIMER_LEFT_131_SELF_END=2.00
PRIMER_RIGHT_131_SELF_END=2.00
PRIMER_LEFT_131_END_STABILITY=6.6000
PRIMER_RIGHT_131_END_STABILITY=11.1000
PRIMER_PAIR_131_COMPL_ANY=4.00
PRIMER_PAIR_131_COMPL_END=1.00
PRIMER_PAIR_131_PRODUCT_SIZE=275
PRIMER_PAIR_131_PRODUCT_TM=78.7
PRIMER_PAIR_132_PENALTY=6.473323
PRIMER_LEFT_132_PENALTY=2.056428
PRIMER_RIGHT_132_PENALTY=4.416895
PRIMER_LEFT_132_SEQUENCE=CCAAAACCTGCATTGATACC
PRIMER_RIGHT_132_SEQUENCE=GTAGGAAGTTTAACAGGATCTCGG
PRIMER_LEFT_132=375,20
PRIMER_RIGHT_132=1296,24
PRIMER_LEFT_132_TM=57.944
PRIMER_RIGHT_132_TM=59.583
PRIMER_LEFT_132_GC_PERCENT=45.000
PRIMER_RIGHT_132_GC_PERCENT=45.833
PRIMER_LEFT_132_SELF_ANY=4.00
PRIMER_RIGHT_132_SELF_ANY=5.00
PRIMER_LEFT_132_SELF_END=2.00
PRIMER_RIGHT_132_SELF_END=2.00
PRIMER_LEFT_132_END_STABILITY=6.8000
PRIMER_RIGHT_132_END_STABILITY=9.9000
PRIMER_PAIR_132_COMPL_ANY=4.00
PRIMER_PAIR_132_COMPL_END=2.00
PRIMER_PAIR_132_PRODUCT_SIZE=922
PRIMER_PAIR_132_PRODUCT_TM=80.8
PRIMER_PAIR_133_PENALTY=6.533439
PRIMER_LEFT_133_PENALTY=2.071163
PRIMER_RIGHT_133_PENALTY=4.462276
PRIMER_LEFT_133_SEQUENCE=ATTGCCATGTTCTGTGGCAG
PRIMER_RIGHT_133_SEQUENCE=CTTGTTCAGAGCACACCTCTCG
PRIMER_LEFT_133=299,20
PRIMER_RIGHT_133=1082,22
PRIMER_LEFT_133_TM=62.071
PRIMER_RIGHT_133_TM=62.462
PRIMER_LEFT_133_GC_PERCENT=50.000
PRIMER_RIGHT_133_GC_PERCENT=54.545
PRIMER_LEFT_133_SELF_ANY=5.00
PRIMER_RIGHT_133_SELF_ANY=5.00
PRIMER_LEFT_133_SELF_END=3.00
PRIMER_RIGHT_133_SELF_END=2.00
PRIMER_LEFT_133_END_STABILITY=9.7000
PRIMER_RIGHT_133_END_STABILITY=8.4000
PRIMER_PAIR_133_COMPL_ANY=5.00
PRIMER_PAIR_133_COMPL_END=1.00
PRIMER_PAIR_133_PRODUCT_SIZE=784
PRIMER_PAIR_133_PRODUCT_TM=80.3
PRIMER_PAIR_134_PENALTY=6.622260
PRIMER_LEFT_134_PENALTY=2.083070
PRIMER_RIGHT_134_PENALTY=4.539189
PRIMER_LEFT_134_SEQUENCE=CATTGATACCAAGGAAGGCAT
PRIMER_RIGHT_134_SEQUENCE=GGTACTCTTCTCACTGCATGTCTC
PRIMER_LEFT_134=385,21
PRIMER_RIGHT_134=688,24
PRIMER_LEFT_134_TM=58.917
PRIMER_RIGHT_134_TM=59.461
PRIMER_LEFT_134_GC_PERCENT=42.857
PRIMER_RIGHT_134_GC_PERCENT=50.000
PRIMER_LEFT_134_SELF_ANY=6.00
PRIMER_RIGHT_134_SELF_ANY=4.00
PRIMER_LEFT_134_SELF_END=2.00
PRIMER_RIGHT_134_SELF_END=0.00
PRIMER_LEFT_134_END_STABILITY=9.6000
PRIMER_RIGHT_134_END_STABILITY=6.1000
PRIMER_PAIR_134_COMPL_ANY=4.00
PRIMER_PAIR_134_COMPL_END=3.00
PRIMER_PAIR_134_PRODUCT_SIZE=304
PRIMER_PAIR_134_PRODUCT_TM=78.7
PRIMER_PAIR_135_PENALTY=6.631312
PRIMER_LEFT_135_PENALTY=3.128205
PRIMER_RIGHT_135_PENALTY=3.503107
PRIMER_LEFT_135_SEQUENCE=GATCAGCTGACTCGCCTGG
PRIMER_RIGHT_135_SEQUENCE=ATCAGTGGGTACCTCCAGC
PRIMER_LEFT_135=1,19
PRIMER_RIGHT_135=268,19
PRIMER_LEFT_135_TM=62.128
PRIMER_RIGHT_135_TM=57.497
PRIMER_LEFT_135_GC_PERCENT=63.158
PRIMER_RIGHT_135_GC_PERCENT=57.895
PRIMER_LEFT_135_SELF_ANY=8.00
PRIMER_RIGHT_135_SELF_ANY=6.00
PRIMER_LEFT_135_SELF_END=3.00
PRIMER_RIGHT_135_SELF_END=2.00
PRIMER_LEFT_135_END_STABILITY=9.7000
PRIMER_RIGHT_135_END_STABILITY=9.7000
PRIMER_PAIR_135_COMPL_ANY=4.00
PRIMER_PAIR_135_COMPL_END=3.00
PRIMER_PAIR_135_PRODUCT_SIZE=268
PRIMER_PAIR_135_PRODUCT_TM=88.1
PRIMER_PAIR_136_PENALTY=6.644573
PRIMER_LEFT_136_PENALTY=2.085443
PRIMER_RIGHT_136_PENALTY=4.559130
PRIMER_LEFT_136_SEQUENCE=CATCAGGGACCAAAACCTG
PRIMER_RIGHT_136_SEQUENCE=TGTCTTCACTCCCATCTGCATAG
PRIMER_LEFT_136=366,19
PRIMER_RIGHT_136=869,23
PRIMER_LEFT_136_TM=58.915
PRIMER_RIGHT_136_TM=61.559
PRIMER_LEFT_136_GC_PERCENT=52.632
PRIMER_RIGHT_136_GC_PERCENT=47.826
PRIMER_LEFT_136_SELF_ANY=4.00
PRIMER_RIGHT_136_SELF_ANY=4.00
PRIMER_LEFT_136_SELF_END=1.00
PRIMER_RIGHT_136_SELF_END=2.00
PRIMER_LEFT_136_END_STABILITY=7.9000
PRIMER_RIGHT_136_END_STABILITY=5.9000
PRIMER_PAIR_136_COMPL_ANY=4.00
PRIMER_PAIR_136_COMPL_END=1.00
PRIMER_PAIR_136_PRODUCT_SIZE=504
PRIMER_PAIR_136_PRODUCT_TM=79.9
PRIMER_PAIR_137_PENALTY=6.682945
PRIMER_LEFT_137_PENALTY=2.118737
PRIMER_RIGHT_137_PENALTY=4.564208
PRIMER_LEFT_137_SEQUENCE=AATGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_137_SEQUENCE=CACACCTCTCGAACCACC
PRIMER_LEFT_137=335,21
PRIMER_RIGHT_137=1071,18
PRIMER_LEFT_137_TM=61.119
PRIMER_RIGHT_137_TM=57.436
PRIMER_LEFT_137_GC_PERCENT=47.619
PRIMER_RIGHT_137_GC_PERCENT=61.111
PRIMER_LEFT_137_SELF_ANY=3.00
PRIMER_RIGHT_137_SELF_ANY=4.00
PRIMER_LEFT_137_SELF_END=0.00
PRIMER_RIGHT_137_SELF_END=0.00
PRIMER_LEFT_137_END_STABILITY=7.9000
PRIMER_RIGHT_137_END_STABILITY=9.4000
PRIMER_PAIR_137_COMPL_ANY=4.00
PRIMER_PAIR_137_COMPL_END=3.00
PRIMER_PAIR_137_PRODUCT_SIZE=737
PRIMER_PAIR_137_PRODUCT_TM=80.3
PRIMER_PAIR_138_PENALTY=6.736909
PRIMER_LEFT_138_PENALTY=2.137489
PRIMER_RIGHT_138_PENALTY=4.599420
PRIMER_LEFT_138_SEQUENCE=GGTACCCACTGATGGTAATGCT
PRIMER_RIGHT_138_SEQUENCE=CTCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_138=256,22
PRIMER_RIGHT_138=684,24
PRIMER_LEFT_138_TM=60.137
PRIMER_RIGHT_138_TM=60.599
PRIMER_LEFT_138_GC_PERCENT=50.000
PRIMER_RIGHT_138_GC_PERCENT=45.833
PRIMER_LEFT_138_SELF_ANY=6.00
PRIMER_RIGHT_138_SELF_ANY=4.00
PRIMER_LEFT_138_SELF_END=0.00
PRIMER_RIGHT_138_SELF_END=0.00
PRIMER_LEFT_138_END_STABILITY=8.1000
PRIMER_RIGHT_138_END_STABILITY=7.3000
PRIMER_PAIR_138_COMPL_ANY=4.00
PRIMER_PAIR_138_COMPL_END=0.00
PRIMER_PAIR_138_PRODUCT_SIZE=429
PRIMER_PAIR_138_PRODUCT_TM=79.4
PRIMER_PAIR_139_PENALTY=6.768315
PRIMER_LEFT_139_PENALTY=2.150273
PRIMER_RIGHT_139_PENALTY=4.618042
PRIMER_LEFT_139_SEQUENCE=CCCCAGATTGCCATGTTC
PRIMER_RIGHT_139_SEQUENCE=TACTTTGTCTTCACTCCCATCTG
PRIMER_LEFT_139=293,18
PRIMER_RIGHT_139=874,23
PRIMER_LEFT_139_TM=59.850
PRIMER_RIGHT_139_TM=58.382
PRIMER_LEFT_139_GC_PERCENT=55.556
PRIMER_RIGHT_139_GC_PERCENT=43.478
PRIMER_LEFT_139_SELF_ANY=4.00
PRIMER_RIGHT_139_SELF_ANY=2.00
PRIMER_LEFT_139_SELF_END=0.00
PRIMER_RIGHT_139_SELF_END=2.00
PRIMER_LEFT_139_END_STABILITY=6.7000
PRIMER_RIGHT_139_END_STABILITY=6.6000
PRIMER_PAIR_139_COMPL_ANY=5.00
PRIMER_PAIR_139_COMPL_END=1.00
PRIMER_PAIR_139_PRODUCT_SIZE=582
PRIMER_PAIR_139_PRODUCT_TM=79.8
PRIMER_PAIR_140_PENALTY=6.800533
PRIMER_LEFT_140_PENALTY=6.438766
PRIMER_RIGHT_140_PENALTY=0.361767
PRIMER_LEFT_140_SEQUENCE=GCAGTATTGCCAAGAAGTCTACCC
PRIMER_RIGHT_140_SEQUENCE=CTCGTCATCATCGGCTTCTT
PRIMER_LEFT_140=409,24
PRIMER_RIGHT_140=937,20
PRIMER_LEFT_140_TM=62.439
PRIMER_RIGHT_140_TM=60.362
PRIMER_LEFT_140_GC_PERCENT=50.000
PRIMER_RIGHT_140_GC_PERCENT=50.000
PRIMER_LEFT_140_SELF_ANY=6.00
PRIMER_RIGHT_140_SELF_ANY=3.00
PRIMER_LEFT_140_SELF_END=0.00
PRIMER_RIGHT_140_SELF_END=0.00
PRIMER_LEFT_140_END_STABILITY=8.4000
PRIMER_RIGHT_140_END_STABILITY=7.0000
PRIMER_PAIR_140_COMPL_ANY=6.00
PRIMER_PAIR_140_COMPL_END=3.00
PRIMER_PAIR_140_PRODUCT_SIZE=529
PRIMER_PAIR_140_PRODUCT_TM=79.8
PRIMER_PAIR_141_PENALTY=6.810499
PRIMER_LEFT_141_PENALTY=2.151267
PRIMER_RIGHT_141_PENALTY=4.659232
PRIMER_LEFT_141_SEQUENCE=AGTGGGATTCAGATCCATCA
PRIMER_RIGHT_141_SEQUENCE=CTACTTTGTCTTCACTCCCATCTG
PRIMER_LEFT_141=351,20
PRIMER_RIGHT_141=875,24
PRIMER_LEFT_141_TM=57.849
PRIMER_RIGHT_141_TM=59.341
PRIMER_LEFT_141_GC_PERCENT=45.000
PRIMER_RIGHT_141_GC_PERCENT=45.833
PRIMER_LEFT_141_SELF_ANY=5.00
PRIMER_RIGHT_141_SELF_ANY=2.00
PRIMER_LEFT_141_SELF_END=2.00
PRIMER_RIGHT_141_SELF_END=2.00
PRIMER_LEFT_141_END_STABILITY=6.9000
PRIMER_RIGHT_141_END_STABILITY=6.6000
PRIMER_PAIR_141_COMPL_ANY=5.00
PRIMER_PAIR_141_COMPL_END=2.00
PRIMER_PAIR_141_PRODUCT_SIZE=525
PRIMER_PAIR_141_PRODUCT_TM=79.8
PRIMER_PAIR_142_PENALTY=6.888757
PRIMER_LEFT_142_PENALTY=3.362783
PRIMER_RIGHT_142_PENALTY=3.525975
PRIMER_LEFT_142_SEQUENCE=CTCGGGCTCCGTCAGTTT
PRIMER_RIGHT_142_SEQUENCE=TTACCATCAGTGGGTACCTCCA
PRIMER_LEFT_142=40,18
PRIMER_RIGHT_142=273,22
PRIMER_LEFT_142_TM=61.363
PRIMER_RIGHT_142_TM=61.526
PRIMER_LEFT_142_GC_PERCENT=61.111
PRIMER_RIGHT_142_GC_PERCENT=50.000
PRIMER_LEFT_142_SELF_ANY=4.00
PRIMER_RIGHT_142_SELF_ANY=6.00
PRIMER_LEFT_142_SELF_END=0.00
PRIMER_RIGHT_142_SELF_END=2.00
PRIMER_LEFT_142_END_STABILITY=6.7000
PRIMER_RIGHT_142_END_STABILITY=8.2000
PRIMER_PAIR_142_COMPL_ANY=2.00
PRIMER_PAIR_142_COMPL_END=1.00
PRIMER_PAIR_142_PRODUCT_SIZE=234
PRIMER_PAIR_142_PRODUCT_TM=87.5
PRIMER_PAIR_143_PENALTY=6.961248
PRIMER_LEFT_143_PENALTY=2.137489
PRIMER_RIGHT_143_PENALTY=4.823759
PRIMER_LEFT_143_SEQUENCE=AGGTACCCACTGATGGTAATGC
PRIMER_RIGHT_143_SEQUENCE=TCTCACTGCATGTCTCTTTGGC
PRIMER_LEFT_143=255,22
PRIMER_RIGHT_143=680,22
PRIMER_LEFT_143_TM=60.137
PRIMER_RIGHT_143_TM=62.824
PRIMER_LEFT_143_GC_PERCENT=50.000
PRIMER_RIGHT_143_GC_PERCENT=50.000
PRIMER_LEFT_143_SELF_ANY=6.00
PRIMER_RIGHT_143_SELF_ANY=4.00
PRIMER_LEFT_143_SELF_END=2.00
PRIMER_RIGHT_143_SELF_END=2.00
PRIMER_LEFT_143_END_STABILITY=8.4000
PRIMER_RIGHT_143_END_STABILITY=10.0000
PRIMER_PAIR_143_COMPL_ANY=4.00
PRIMER_PAIR_143_COMPL_END=2.00
PRIMER_PAIR_143_PRODUCT_SIZE=426
PRIMER_PAIR_143_PRODUCT_TM=79.4
PRIMER_PAIR_144_PENALTY=6.975656
PRIMER_LEFT_144_PENALTY=6.610485
PRIMER_RIGHT_144_PENALTY=0.365170
PRIMER_LEFT_144_SEQUENCE=TTGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_144_SEQUENCE=CGTAAAAGAATGGGGCACAC
PRIMER_LEFT_144=415,24
PRIMER_RIGHT_144=1163,20
PRIMER_LEFT_144_TM=62.610
PRIMER_RIGHT_144_TM=60.365
PRIMER_LEFT_144_GC_PERCENT=45.833
PRIMER_RIGHT_144_GC_PERCENT=50.000
PRIMER_LEFT_144_SELF_ANY=5.00
PRIMER_RIGHT_144_SELF_ANY=3.00
PRIMER_LEFT_144_SELF_END=1.00
PRIMER_RIGHT_144_SELF_END=1.00
PRIMER_LEFT_144_END_STABILITY=6.4000
PRIMER_RIGHT_144_END_STABILITY=6.4000
PRIMER_PAIR_144_COMPL_ANY=4.00
PRIMER_PAIR_144_COMPL_END=2.00
PRIMER_PAIR_144_PRODUCT_SIZE=749
PRIMER_PAIR_144_PRODUCT_TM=80.7
PRIMER_PAIR_145_PENALTY=6.992001
PRIMER_LEFT_145_PENALTY=2.150151
PRIMER_RIGHT_145_PENALTY=4.841851
PRIMER_LEFT_145_SEQUENCE=ATGAATGTCCAGAATGGGAAG
PRIMER_RIGHT_145_SEQUENCE=AAACTCACCAACTAAGCAGCGG
PRIMER_LEFT_145=332,21
PRIMER_RIGHT_145=565,22
PRIMER_LEFT_145_TM=58.850
PRIMER_RIGHT_145_TM=62.842
PRIMER_LEFT_145_GC_PERCENT=42.857
PRIMER_RIGHT_145_GC_PERCENT=50.000
PRIMER_LEFT_145_SELF_ANY=3.00
PRIMER_RIGHT_145_SELF_ANY=3.00
PRIMER_LEFT_145_SELF_END=0.00
PRIMER_RIGHT_145_SELF_END=2.00
PRIMER_LEFT_145_END_STABILITY=8.2000
PRIMER_RIGHT_145_END_STABILITY=11.4000
PRIMER_PAIR_145_COMPL_ANY=3.00
PRIMER_PAIR_145_COMPL_END=0.00
PRIMER_PAIR_145_PRODUCT_SIZE=234
PRIMER_PAIR_145_PRODUCT_TM=78.2
PRIMER_PAIR_146_PENALTY=7.015504
PRIMER_LEFT_146_PENALTY=6.638755
PRIMER_RIGHT_146_PENALTY=0.376749
PRIMER_LEFT_146_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_146_SEQUENCE=CTCTCGAACCACCTCTTCCA
PRIMER_LEFT_146=411,25
PRIMER_RIGHT_146=1066,20
PRIMER_LEFT_146_TM=61.639
PRIMER_RIGHT_146_TM=60.377
PRIMER_LEFT_146_GC_PERCENT=44.000
PRIMER_RIGHT_146_GC_PERCENT=55.000
PRIMER_LEFT_146_SELF_ANY=5.00
PRIMER_RIGHT_146_SELF_ANY=4.00
PRIMER_LEFT_146_SELF_END=1.00
PRIMER_RIGHT_146_SELF_END=0.00
PRIMER_LEFT_146_END_STABILITY=8.2000
PRIMER_RIGHT_146_END_STABILITY=8.5000
PRIMER_PAIR_146_COMPL_ANY=5.00
PRIMER_PAIR_146_COMPL_END=1.00
PRIMER_PAIR_146_PRODUCT_SIZE=656
PRIMER_PAIR_146_PRODUCT_TM=80.4
PRIMER_PAIR_147_PENALTY=7.063424
PRIMER_LEFT_147_PENALTY=2.171836
PRIMER_RIGHT_147_PENALTY=4.891588
PRIMER_LEFT_147_SEQUENCE=CACATGAATGTCCAGAATGGG
PRIMER_RIGHT_147_SEQUENCE=CACACCTCTCGAACCACCTCTT
PRIMER_LEFT_147=329,21
PRIMER_RIGHT_147=1071,22
PRIMER_LEFT_147_TM=61.172
PRIMER_RIGHT_147_TM=62.892
PRIMER_LEFT_147_GC_PERCENT=47.619
PRIMER_RIGHT_147_GC_PERCENT=54.545
PRIMER_LEFT_147_SELF_ANY=6.00
PRIMER_RIGHT_147_SELF_ANY=4.00
PRIMER_LEFT_147_SELF_END=1.00
PRIMER_RIGHT_147_SELF_END=0.00
PRIMER_LEFT_147_END_STABILITY=9.6000
PRIMER_RIGHT_147_END_STABILITY=6.7000
PRIMER_PAIR_147_COMPL_ANY=4.00
PRIMER_PAIR_147_COMPL_END=2.00
PRIMER_PAIR_147_PRODUCT_SIZE=743
PRIMER_PAIR_147_PRODUCT_TM=80.3
PRIMER_PAIR_148_PENALTY=7.093953
PRIMER_LEFT_148_PENALTY=2.174817
PRIMER_RIGHT_148_PENALTY=4.919136
PRIMER_LEFT_148_SEQUENCE=ACCAAAACCTGCATTGATACC
PRIMER_RIGHT_148_SEQUENCE=ACTTTGTCTTCACTCCCATCTGC
PRIMER_LEFT_148=374,21
PRIMER_RIGHT_148=873,23
PRIMER_LEFT_148_TM=58.825
PRIMER_RIGHT_148_TM=61.919
PRIMER_LEFT_148_GC_PERCENT=42.857
PRIMER_RIGHT_148_GC_PERCENT=47.826
PRIMER_LEFT_148_SELF_ANY=4.00
PRIMER_RIGHT_148_SELF_ANY=2.00
PRIMER_LEFT_148_SELF_END=2.00
PRIMER_RIGHT_148_SELF_END=2.00
PRIMER_LEFT_148_END_STABILITY=6.8000
PRIMER_RIGHT_148_END_STABILITY=8.2000
PRIMER_PAIR_148_COMPL_ANY=4.00
PRIMER_PAIR_148_COMPL_END=0.00
PRIMER_PAIR_148_PRODUCT_SIZE=500
PRIMER_PAIR_148_PRODUCT_TM=79.7
PRIMER_PAIR_149_PENALTY=7.107233
PRIMER_LEFT_149_PENALTY=2.186531
PRIMER_RIGHT_149_PENALTY=4.920702
PRIMER_LEFT_149_SEQUENCE=AGGGACCAAAACCTGCATTG
PRIMER_RIGHT_149_SEQUENCE=GAGTAAACTTTGGGACATGGCG
PRIMER_LEFT_149=370,20
PRIMER_RIGHT_149=1249,22
PRIMER_LEFT_149_TM=62.187
PRIMER_RIGHT_149_TM=62.921
PRIMER_LEFT_149_GC_PERCENT=50.000
PRIMER_RIGHT_149_GC_PERCENT=50.000
PRIMER_LEFT_149_SELF_ANY=4.00
PRIMER_RIGHT_149_SELF_ANY=5.00
PRIMER_LEFT_149_SELF_END=3.00
PRIMER_RIGHT_149_SELF_END=2.00
PRIMER_LEFT_149_END_STABILITY=7.2000
PRIMER_RIGHT_149_END_STABILITY=11.7000
PRIMER_PAIR_149_COMPL_ANY=5.00
PRIMER_PAIR_149_COMPL_END=1.00
PRIMER_PAIR_149_PRODUCT_SIZE=880
PRIMER_PAIR_149_PRODUCT_TM=80.9
PRIMER_PAIR_150_PENALTY=7.187746
PRIMER_LEFT_150_PENALTY=2.186531
PRIMER_RIGHT_150_PENALTY=5.001215
PRIMER_LEFT_150_SEQUENCE=CAGGGACCAAAACCTGCATT
PRIMER_RIGHT_150_SEQUENCE=CTGGCTGCTGTTGTAGGAAGTTT
PRIMER_LEFT_150=369,20
PRIMER_RIGHT_150=1308,23
PRIMER_LEFT_150_TM=62.187
PRIMER_RIGHT_150_TM=62.001
PRIMER_LEFT_150_GC_PERCENT=50.000
PRIMER_RIGHT_150_GC_PERCENT=47.826
PRIMER_LEFT_150_SELF_ANY=4.00
PRIMER_RIGHT_150_SELF_ANY=3.00
PRIMER_LEFT_150_SELF_END=1.00
PRIMER_RIGHT_150_SELF_END=2.00
PRIMER_LEFT_150_END_STABILITY=8.4000
PRIMER_RIGHT_150_END_STABILITY=6.7000
PRIMER_PAIR_150_COMPL_ANY=4.00
PRIMER_PAIR_150_COMPL_END=2.00
PRIMER_PAIR_150_PRODUCT_SIZE=940
PRIMER_PAIR_150_PRODUCT_TM=80.9
PRIMER_PAIR_151_PENALTY=7.220368
PRIMER_LEFT_151_PENALTY=2.203644
PRIMER_RIGHT_151_PENALTY=5.016724
PRIMER_LEFT_151_SEQUENCE=CTGGAGGTACCCACTGATGGT
PRIMER_RIGHT_151_SEQUENCE=ACTCACCAACTAAGCAGCGGTAG
PRIMER_LEFT_151=251,21
PRIMER_RIGHT_151=563,23
PRIMER_LEFT_151_TM=61.204
PRIMER_RIGHT_151_TM=62.017
PRIMER_LEFT_151_GC_PERCENT=57.143
PRIMER_RIGHT_151_GC_PERCENT=52.174
PRIMER_LEFT_151_SELF_ANY=6.00
PRIMER_RIGHT_151_SELF_ANY=3.00
PRIMER_LEFT_151_SELF_END=3.00
PRIMER_RIGHT_151_SELF_END=3.00
PRIMER_LEFT_151_END_STABILITY=7.8000
PRIMER_RIGHT_151_END_STABILITY=6.9000
PRIMER_PAIR_151_COMPL_ANY=4.00
PRIMER_PAIR_151_COMPL_END=3.00
PRIMER_PAIR_151_PRODUCT_SIZE=313
PRIMER_PAIR_151_PRODUCT_TM=79.5
PRIMER_PAIR_152_PENALTY=7.271327
PRIMER_LEFT_152_PENALTY=2.207202
PRIMER_RIGHT_152_PENALTY=5.064124
PRIMER_LEFT_152_SEQUENCE=GAATGGGAAGTGGGATTCAGA
PRIMER_RIGHT_152_SEQUENCE=TTGTAGGAAGTTTAACAGGATCTCG
PRIMER_LEFT_152=343,21
PRIMER_RIGHT_152=1298,25
PRIMER_LEFT_152_TM=61.207
PRIMER_RIGHT_152_TM=60.064
PRIMER_LEFT_152_GC_PERCENT=47.619
PRIMER_RIGHT_152_GC_PERCENT=40.000
PRIMER_LEFT_152_SELF_ANY=4.00
PRIMER_RIGHT_152_SELF_ANY=5.00
PRIMER_LEFT_152_SELF_END=3.00
PRIMER_RIGHT_152_SELF_END=2.00
PRIMER_LEFT_152_END_STABILITY=6.7000
PRIMER_RIGHT_152_END_STABILITY=8.4000
PRIMER_PAIR_152_COMPL_ANY=3.00
PRIMER_PAIR_152_COMPL_END=2.00
PRIMER_PAIR_152_PRODUCT_SIZE=956
PRIMER_PAIR_152_PRODUCT_TM=80.8
PRIMER_PAIR_153_PENALTY=7.293981
PRIMER_LEFT_153_PENALTY=2.225062
PRIMER_RIGHT_153_PENALTY=5.068920
PRIMER_LEFT_153_SEQUENCE=ATGAATGTCCAGAATGGGAA
PRIMER_RIGHT_153_SEQUENCE=GCTGTTGTAGGAAGTTTAACAGGA
PRIMER_LEFT_153=332,20
PRIMER_RIGHT_153=1302,24
PRIMER_LEFT_153_TM=57.775
PRIMER_RIGHT_153_TM=58.931
PRIMER_LEFT_153_GC_PERCENT=40.000
PRIMER_RIGHT_153_GC_PERCENT=41.667
PRIMER_LEFT_153_SELF_ANY=3.00
PRIMER_RIGHT_153_SELF_ANY=5.00
PRIMER_LEFT_153_SELF_END=1.00
PRIMER_RIGHT_153_SELF_END=0.00
PRIMER_LEFT_153_END_STABILITY=9.7000
PRIMER_RIGHT_153_END_STABILITY=8.2000
PRIMER_PAIR_153_COMPL_ANY=3.00
PRIMER_PAIR_153_COMPL_END=0.00
PRIMER_PAIR_153_PRODUCT_SIZE=971
PRIMER_PAIR_153_PRODUCT_TM=80.7
PRIMER_PAIR_154_PENALTY=7.330509
PRIMER_LEFT_154_PENALTY=6.944753
PRIMER_RIGHT_154_PENALTY=0.385756
PRIMER_LEFT_154_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_154_SEQUENCE=TCCACCTCAGCCACTTCTTC
PRIMER_LEFT_154=413,25
PRIMER_RIGHT_154=912,20
PRIMER_LEFT_154_TM=61.945
PRIMER_RIGHT_154_TM=60.386
PRIMER_LEFT_154_GC_PERCENT=44.000
PRIMER_RIGHT_154_GC_PERCENT=55.000
PRIMER_LEFT_154_SELF_ANY=5.00
PRIMER_RIGHT_154_SELF_ANY=3.00
PRIMER_LEFT_154_SELF_END=0.00
PRIMER_RIGHT_154_SELF_END=0.00
PRIMER_LEFT_154_END_STABILITY=6.7000
PRIMER_RIGHT_154_END_STABILITY=6.7000
PRIMER_PAIR_154_COMPL_ANY=7.00
PRIMER_PAIR_154_COMPL_END=2.00
PRIMER_PAIR_154_PRODUCT_SIZE=500
PRIMER_PAIR_154_PRODUCT_TM=79.8
PRIMER_PAIR_155_PENALTY=7.330509
PRIMER_LEFT_155_PENALTY=6.944753
PRIMER_RIGHT_155_PENALTY=0.385756
PRIMER_LEFT_155_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_155_SEQUENCE=TCTTCCACCTCAGCCACTTC
PRIMER_LEFT_155=412,25
PRIMER_RIGHT_155=915,20
PRIMER_LEFT_155_TM=61.945
PRIMER_RIGHT_155_TM=60.386
PRIMER_LEFT_155_GC_PERCENT=44.000
PRIMER_RIGHT_155_GC_PERCENT=55.000
PRIMER_LEFT_155_SELF_ANY=5.00
PRIMER_RIGHT_155_SELF_ANY=3.00
PRIMER_LEFT_155_SELF_END=0.00
PRIMER_RIGHT_155_SELF_END=0.00
PRIMER_LEFT_155_END_STABILITY=7.0000
PRIMER_RIGHT_155_END_STABILITY=6.4000
PRIMER_PAIR_155_COMPL_ANY=5.00
PRIMER_PAIR_155_COMPL_END=3.00
PRIMER_PAIR_155_PRODUCT_SIZE=504
PRIMER_PAIR_155_PRODUCT_TM=79.8
PRIMER_PAIR_156_PENALTY=7.336644
PRIMER_LEFT_156_PENALTY=2.222066
PRIMER_RIGHT_156_PENALTY=5.114578
PRIMER_LEFT_156_SEQUENCE=GGAGGTACCCACTGATGGTAA
PRIMER_RIGHT_156_SEQUENCE=CTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_156=253,21
PRIMER_RIGHT_156=684,23
PRIMER_LEFT_156_TM=58.778
PRIMER_RIGHT_156_TM=57.885
PRIMER_LEFT_156_GC_PERCENT=52.381
PRIMER_RIGHT_156_GC_PERCENT=43.478
PRIMER_LEFT_156_SELF_ANY=6.00
PRIMER_RIGHT_156_SELF_ANY=4.00
PRIMER_LEFT_156_SELF_END=3.00
PRIMER_RIGHT_156_SELF_END=0.00
PRIMER_LEFT_156_END_STABILITY=7.2000
PRIMER_RIGHT_156_END_STABILITY=7.0000
PRIMER_PAIR_156_COMPL_ANY=4.00
PRIMER_PAIR_156_COMPL_END=2.00
PRIMER_PAIR_156_PRODUCT_SIZE=432
PRIMER_PAIR_156_PRODUCT_TM=79.5
PRIMER_PAIR_157_PENALTY=7.340305
PRIMER_LEFT_157_PENALTY=2.227266
PRIMER_RIGHT_157_PENALTY=5.113039
PRIMER_LEFT_157_SEQUENCE=GTCCAGAATGGGAAGTGGGA
PRIMER_RIGHT_157_SEQUENCE=TGTAGGAAGTTTAACAGGATCTCG
PRIMER_LEFT_157=338,20
PRIMER_RIGHT_157=1297,24
PRIMER_LEFT_157_TM=62.227
PRIMER_RIGHT_157_TM=58.887
PRIMER_LEFT_157_GC_PERCENT=55.000
PRIMER_RIGHT_157_GC_PERCENT=41.667
PRIMER_LEFT_157_SELF_ANY=3.00
PRIMER_RIGHT_157_SELF_ANY=5.00
PRIMER_LEFT_157_SELF_END=0.00
PRIMER_RIGHT_157_SELF_END=2.00
PRIMER_LEFT_157_END_STABILITY=9.7000
PRIMER_RIGHT_157_END_STABILITY=8.4000
PRIMER_PAIR_157_COMPL_ANY=3.00
PRIMER_PAIR_157_COMPL_END=1.00
PRIMER_PAIR_157_PRODUCT_SIZE=960
PRIMER_PAIR_157_PRODUCT_TM=80.8
PRIMER_PAIR_158_PENALTY=7.394664
PRIMER_LEFT_158_PENALTY=2.292791
PRIMER_RIGHT_158_PENALTY=5.101873
PRIMER_LEFT_158_SEQUENCE=GGGACCAAAACCTGCATTG
PRIMER_RIGHT_158_SEQUENCE=ACTACTTTGTCTTCACTCCCATCTG
PRIMER_LEFT_158=371,19
PRIMER_RIGHT_158=876,25
PRIMER_LEFT_158_TM=61.293
PRIMER_RIGHT_158_TM=60.102
PRIMER_LEFT_158_GC_PERCENT=52.632
PRIMER_RIGHT_158_GC_PERCENT=44.000
PRIMER_LEFT_158_SELF_ANY=4.00
PRIMER_RIGHT_158_SELF_ANY=2.00
PRIMER_LEFT_158_SELF_END=3.00
PRIMER_RIGHT_158_SELF_END=2.00
PRIMER_LEFT_158_END_STABILITY=7.2000
PRIMER_RIGHT_158_END_STABILITY=6.6000
PRIMER_PAIR_158_COMPL_ANY=4.00
PRIMER_PAIR_158_COMPL_END=1.00
PRIMER_PAIR_158_PRODUCT_SIZE=506
PRIMER_PAIR_158_PRODUCT_TM=79.8
PRIMER_PAIR_159_PENALTY=7.474704
PRIMER_LEFT_159_PENALTY=3.683571
PRIMER_RIGHT_159_PENALTY=3.791133
PRIMER_LEFT_159_SEQUENCE=AGCGGTAGGCGAGAGCAC
PRIMER_RIGHT_159_SEQUENCE=CAGGGTAGACTTCTTGGCAATAC
PRIMER_LEFT_159=65,18
PRIMER_RIGHT_159=434,23
PRIMER_LEFT_159_TM=61.684
PRIMER_RIGHT_159_TM=59.209
PRIMER_LEFT_159_GC_PERCENT=66.667
PRIMER_RIGHT_159_GC_PERCENT=47.826
PRIMER_LEFT_159_SELF_ANY=2.00
PRIMER_RIGHT_159_SELF_ANY=5.00
PRIMER_LEFT_159_SELF_END=2.00
PRIMER_RIGHT_159_SELF_END=3.00
PRIMER_LEFT_159_END_STABILITY=7.9000
PRIMER_RIGHT_159_END_STABILITY=5.6000
PRIMER_PAIR_159_COMPL_ANY=3.00
PRIMER_PAIR_159_COMPL_END=1.00
PRIMER_PAIR_159_PRODUCT_SIZE=370
PRIMER_PAIR_159_PRODUCT_TM=84.5
PRIMER_PAIR_160_PENALTY=7.497858
PRIMER_LEFT_160_PENALTY=2.303792
PRIMER_RIGHT_160_PENALTY=5.194065
PRIMER_LEFT_160_SEQUENCE=ATGAATGTCCAGAATGGGAAGT
PRIMER_RIGHT_160_SEQUENCE=CACTTACAAACTCACCAACTAAGCA
PRIMER_LEFT_160=332,22
PRIMER_RIGHT_160=572,25
PRIMER_LEFT_160_TM=59.696
PRIMER_RIGHT_160_TM=59.806
PRIMER_LEFT_160_GC_PERCENT=40.909
PRIMER_RIGHT_160_GC_PERCENT=40.000
PRIMER_LEFT_160_SELF_ANY=3.00
PRIMER_RIGHT_160_SELF_ANY=4.00
PRIMER_LEFT_160_SELF_END=1.00
PRIMER_RIGHT_160_SELF_END=0.00
PRIMER_LEFT_160_END_STABILITY=6.4000
PRIMER_RIGHT_160_END_STABILITY=8.5000
PRIMER_PAIR_160_COMPL_ANY=4.00
PRIMER_PAIR_160_COMPL_END=2.00
PRIMER_PAIR_160_PRODUCT_SIZE=241
PRIMER_PAIR_160_PRODUCT_TM=78.2
PRIMER_PAIR_161_PENALTY=7.534720
PRIMER_LEFT_161_PENALTY=3.708825
PRIMER_RIGHT_161_PENALTY=3.825895
PRIMER_LEFT_161_SEQUENCE=GGCTCCGTCAGTTTCCTC
PRIMER_RIGHT_161_SEQUENCE=TGCAGTTCAGGGTAGACTTCTTG
PRIMER_LEFT_161=44,18
PRIMER_RIGHT_161=441,23
PRIMER_LEFT_161_TM=58.291
PRIMER_RIGHT_161_TM=60.826
PRIMER_LEFT_161_GC_PERCENT=61.111
PRIMER_RIGHT_161_GC_PERCENT=47.826
PRIMER_LEFT_161_SELF_ANY=2.00
PRIMER_RIGHT_161_SELF_ANY=4.00
PRIMER_LEFT_161_SELF_END=0.00
PRIMER_RIGHT_161_SELF_END=1.00
PRIMER_LEFT_161_END_STABILITY=7.9000
PRIMER_RIGHT_161_END_STABILITY=7.0000
PRIMER_PAIR_161_COMPL_ANY=3.00
PRIMER_PAIR_161_COMPL_END=1.00
PRIMER_PAIR_161_PRODUCT_SIZE=398
PRIMER_PAIR_161_PRODUCT_TM=84.6
PRIMER_PAIR_162_PENALTY=7.571660
PRIMER_LEFT_162_PENALTY=2.315468
PRIMER_RIGHT_162_PENALTY=5.256192
PRIMER_LEFT_162_SEQUENCE=CTGATGGTAATGCTGGCCT
PRIMER_RIGHT_162_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_162=264,19
PRIMER_RIGHT_162=685,24
PRIMER_LEFT_162_TM=58.685
PRIMER_RIGHT_162_TM=58.744
PRIMER_LEFT_162_GC_PERCENT=52.632
PRIMER_RIGHT_162_GC_PERCENT=41.667
PRIMER_LEFT_162_SELF_ANY=4.00
PRIMER_RIGHT_162_SELF_ANY=4.00
PRIMER_LEFT_162_SELF_END=2.00
PRIMER_RIGHT_162_SELF_END=0.00
PRIMER_LEFT_162_END_STABILITY=10.9000
PRIMER_RIGHT_162_END_STABILITY=7.0000
PRIMER_PAIR_162_COMPL_ANY=4.00
PRIMER_PAIR_162_COMPL_END=0.00
PRIMER_PAIR_162_PRODUCT_SIZE=422
PRIMER_PAIR_162_PRODUCT_TM=79.3
PRIMER_PAIR_163_PENALTY=7.656291
PRIMER_LEFT_163_PENALTY=3.791523
PRIMER_RIGHT_163_PENALTY=3.864768
PRIMER_LEFT_163_SEQUENCE=AGCTGACTCGCCTGGCTCT
PRIMER_RIGHT_163_SEQUENCE=GCAGTTCAGGGTAGACTTCTTG
PRIMER_LEFT_163=5,19
PRIMER_RIGHT_163=440,22
PRIMER_LEFT_163_TM=62.792
PRIMER_RIGHT_163_TM=58.135
PRIMER_LEFT_163_GC_PERCENT=63.158
PRIMER_RIGHT_163_GC_PERCENT=50.000
PRIMER_LEFT_163_SELF_ANY=5.00
PRIMER_RIGHT_163_SELF_ANY=4.00
PRIMER_LEFT_163_SELF_END=0.00
PRIMER_RIGHT_163_SELF_END=1.00
PRIMER_LEFT_163_END_STABILITY=7.9000
PRIMER_RIGHT_163_END_STABILITY=7.0000
PRIMER_PAIR_163_COMPL_ANY=4.00
PRIMER_PAIR_163_COMPL_END=2.00
PRIMER_PAIR_163_PRODUCT_SIZE=436
PRIMER_PAIR_163_PRODUCT_TM=85.2
PRIMER_PAIR_164_PENALTY=7.663983
PRIMER_LEFT_164_PENALTY=2.325240
PRIMER_RIGHT_164_PENALTY=5.338743
PRIMER_LEFT_164_SEQUENCE=GCACATGAATGTCCAGAATGG
PRIMER_RIGHT_164_SEQUENCE=AACTCACCAACTAAGCAGCGGTA
PRIMER_LEFT_164=328,21
PRIMER_RIGHT_164=564,23
PRIMER_LEFT_164_TM=61.325
PRIMER_RIGHT_164_TM=62.339
PRIMER_LEFT_164_GC_PERCENT=47.619
PRIMER_RIGHT_164_GC_PERCENT=47.826
PRIMER_LEFT_164_SELF_ANY=6.00
PRIMER_RIGHT_164_SELF_ANY=3.00
PRIMER_LEFT_164_SELF_END=3.00
PRIMER_RIGHT_164_SELF_END=2.00
PRIMER_LEFT_164_END_STABILITY=8.4000
PRIMER_RIGHT_164_END_STABILITY=8.9000
PRIMER_PAIR_164_COMPL_ANY=3.00
PRIMER_PAIR_164_COMPL_END=0.00
PRIMER_PAIR_164_PRODUCT_SIZE=237
PRIMER_PAIR_164_PRODUCT_TM=78.5
PRIMER_PAIR_165_PENALTY=7.738336
PRIMER_LEFT_165_PENALTY=3.873568
PRIMER_RIGHT_165_PENALTY=3.864768
PRIMER_LEFT_165_SEQUENCE=GCTGACTCGCCTGGCTCT
PRIMER_RIGHT_165_SEQUENCE=TGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_165=6,18
PRIMER_RIGHT_165=441,22
PRIMER_LEFT_165_TM=61.874
PRIMER_RIGHT_165_TM=58.135
PRIMER_LEFT_165_GC_PERCENT=66.667
PRIMER_RIGHT_165_GC_PERCENT=45.455
PRIMER_LEFT_165_SELF_ANY=5.00
PRIMER_RIGHT_165_SELF_ANY=4.00
PRIMER_LEFT_165_SELF_END=0.00
PRIMER_RIGHT_165_SELF_END=2.00
PRIMER_LEFT_165_END_STABILITY=7.9000
PRIMER_RIGHT_165_END_STABILITY=7.0000
PRIMER_PAIR_165_COMPL_ANY=4.00
PRIMER_PAIR_165_COMPL_END=1.00
PRIMER_PAIR_165_PRODUCT_SIZE=436
PRIMER_PAIR_165_PRODUCT_TM=85.2
PRIMER_PAIR_166_PENALTY=7.741586
PRIMER_LEFT_166_PENALTY=2.376315
PRIMER_RIGHT_166_PENALTY=5.365271
PRIMER_LEFT_166_SEQUENCE=GGTAATGCTGGCCTGCTG
PRIMER_RIGHT_166_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCT
PRIMER_LEFT_166=269,18
PRIMER_RIGHT_166=688,25
PRIMER_LEFT_166_TM=60.376
PRIMER_RIGHT_166_TM=60.365
PRIMER_LEFT_166_GC_PERCENT=61.111
PRIMER_RIGHT_166_GC_PERCENT=48.000
PRIMER_LEFT_166_SELF_ANY=6.00
PRIMER_RIGHT_166_SELF_ANY=4.00
PRIMER_LEFT_166_SELF_END=2.00
PRIMER_RIGHT_166_SELF_END=0.00
PRIMER_LEFT_166_END_STABILITY=8.5000
PRIMER_RIGHT_166_END_STABILITY=6.4000
PRIMER_PAIR_166_COMPL_ANY=4.00
PRIMER_PAIR_166_COMPL_END=0.00
PRIMER_PAIR_166_PRODUCT_SIZE=420
PRIMER_PAIR_166_PRODUCT_TM=79.4
PRIMER_PAIR_167_PENALTY=7.762954
PRIMER_LEFT_167_PENALTY=3.873568
PRIMER_RIGHT_167_PENALTY=3.889386
PRIMER_LEFT_167_SEQUENCE=AGCTGACTCGCCTGGCTC
PRIMER_RIGHT_167_SEQUENCE=CTGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_167=5,18
PRIMER_RIGHT_167=442,23
PRIMER_LEFT_167_TM=61.874
PRIMER_RIGHT_167_TM=59.111
PRIMER_LEFT_167_GC_PERCENT=66.667
PRIMER_RIGHT_167_GC_PERCENT=47.826
PRIMER_LEFT_167_SELF_ANY=5.00
PRIMER_RIGHT_167_SELF_ANY=6.00
PRIMER_LEFT_167_SELF_END=1.00
PRIMER_RIGHT_167_SELF_END=2.00
PRIMER_LEFT_167_END_STABILITY=9.4000
PRIMER_RIGHT_167_END_STABILITY=7.0000
PRIMER_PAIR_167_COMPL_ANY=4.00
PRIMER_PAIR_167_COMPL_END=2.00
PRIMER_PAIR_167_PRODUCT_SIZE=438
PRIMER_PAIR_167_PRODUCT_TM=85.2
PRIMER_PAIR_168_PENALTY=7.783163
PRIMER_LEFT_168_PENALTY=2.389824
PRIMER_RIGHT_168_PENALTY=5.393340
PRIMER_LEFT_168_SEQUENCE=TTGATACCAAGGAAGGCATC
PRIMER_RIGHT_168_SEQUENCE=GTCTTCACTCCCATCTGCATAGTC
PRIMER_LEFT_168=387,20
PRIMER_RIGHT_168=868,24
PRIMER_LEFT_168_TM=57.610
PRIMER_RIGHT_168_TM=61.393
PRIMER_LEFT_168_GC_PERCENT=45.000
PRIMER_RIGHT_168_GC_PERCENT=50.000
PRIMER_LEFT_168_SELF_ANY=6.00
PRIMER_RIGHT_168_SELF_ANY=4.00
PRIMER_LEFT_168_SELF_END=3.00
PRIMER_RIGHT_168_SELF_END=1.00
PRIMER_LEFT_168_END_STABILITY=8.1000
PRIMER_RIGHT_168_END_STABILITY=5.4000
PRIMER_PAIR_168_COMPL_ANY=4.00
PRIMER_PAIR_168_COMPL_END=0.00
PRIMER_PAIR_168_PRODUCT_SIZE=482
PRIMER_PAIR_168_PRODUCT_TM=79.8
PRIMER_PAIR_169_PENALTY=7.823941
PRIMER_LEFT_169_PENALTY=7.438185
PRIMER_RIGHT_169_PENALTY=0.385756
PRIMER_LEFT_169_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_169_SEQUENCE=TCTTCTTCCACCTCAGCCAC
PRIMER_LEFT_169=410,25
PRIMER_RIGHT_169=918,20
PRIMER_LEFT_169_TM=62.438
PRIMER_RIGHT_169_TM=60.386
PRIMER_LEFT_169_GC_PERCENT=48.000
PRIMER_RIGHT_169_GC_PERCENT=55.000
PRIMER_LEFT_169_SELF_ANY=5.00
PRIMER_RIGHT_169_SELF_ANY=3.00
PRIMER_LEFT_169_SELF_END=1.00
PRIMER_RIGHT_169_SELF_END=0.00
PRIMER_LEFT_169_END_STABILITY=9.7000
PRIMER_RIGHT_169_END_STABILITY=9.4000
PRIMER_PAIR_169_COMPL_ANY=6.00
PRIMER_PAIR_169_COMPL_END=1.00
PRIMER_PAIR_169_PRODUCT_SIZE=509
PRIMER_PAIR_169_PRODUCT_TM=79.7
PRIMER_PAIR_170_PENALTY=7.911309
PRIMER_LEFT_170_PENALTY=3.902978
PRIMER_RIGHT_170_PENALTY=4.008330
PRIMER_LEFT_170_SEQUENCE=CTGACTCGCCTGGCTCTGA
PRIMER_RIGHT_170_SEQUENCE=CAGTTCAGGGTAGACTTCTTGG
PRIMER_LEFT_170=7,19
PRIMER_RIGHT_170=439,22
PRIMER_LEFT_170_TM=62.903
PRIMER_RIGHT_170_TM=57.992
PRIMER_LEFT_170_GC_PERCENT=63.158
PRIMER_RIGHT_170_GC_PERCENT=50.000
PRIMER_LEFT_170_SELF_ANY=5.00
PRIMER_RIGHT_170_SELF_ANY=4.00
PRIMER_LEFT_170_SELF_END=3.00
PRIMER_RIGHT_170_SELF_END=0.00
PRIMER_LEFT_170_END_STABILITY=6.7000
PRIMER_RIGHT_170_END_STABILITY=8.5000
PRIMER_PAIR_170_COMPL_ANY=4.00
PRIMER_PAIR_170_COMPL_END=0.00
PRIMER_PAIR_170_PRODUCT_SIZE=433
PRIMER_PAIR_170_PRODUCT_TM=85.2
PRIMER_PAIR_171_PENALTY=7.950578
PRIMER_LEFT_171_PENALTY=7.562338
PRIMER_RIGHT_171_PENALTY=0.388240
PRIMER_LEFT_171_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTACC
PRIMER_RIGHT_171_SEQUENCE=TCTGTGGCTTCTTCGTAGGG
PRIMER_LEFT_171=407,25
PRIMER_RIGHT_171=999,20
PRIMER_LEFT_171_TM=62.562
PRIMER_RIGHT_171_TM=60.388
PRIMER_LEFT_171_GC_PERCENT=48.000
PRIMER_RIGHT_171_GC_PERCENT=55.000
PRIMER_LEFT_171_SELF_ANY=6.00
PRIMER_RIGHT_171_SELF_ANY=4.00
PRIMER_LEFT_171_SELF_END=1.00
PRIMER_RIGHT_171_SELF_END=0.00
PRIMER_LEFT_171_END_STABILITY=6.9000
PRIMER_RIGHT_171_END_STABILITY=8.7000
PRIMER_PAIR_171_COMPL_ANY=6.00
PRIMER_PAIR_171_COMPL_END=2.00
PRIMER_PAIR_171_PRODUCT_SIZE=593
PRIMER_PAIR_171_PRODUCT_TM=80.0
PRIMER_PAIR_172_PENALTY=7.971303
PRIMER_LEFT_172_PENALTY=7.562338
PRIMER_RIGHT_172_PENALTY=0.408965
PRIMER_LEFT_172_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTAC
PRIMER_RIGHT_172_SEQUENCE=TCATCGTCCTCGTCATCATC
PRIMER_LEFT_172=406,25
PRIMER_RIGHT_172=945,20
PRIMER_LEFT_172_TM=62.562
PRIMER_RIGHT_172_TM=59.591
PRIMER_LEFT_172_GC_PERCENT=48.000
PRIMER_RIGHT_172_GC_PERCENT=50.000
PRIMER_LEFT_172_SELF_ANY=6.00
PRIMER_RIGHT_172_SELF_ANY=3.00
PRIMER_LEFT_172_SELF_END=3.00
PRIMER_RIGHT_172_SELF_END=1.00
PRIMER_LEFT_172_END_STABILITY=5.4000
PRIMER_RIGHT_172_END_STABILITY=6.6000
PRIMER_PAIR_172_COMPL_ANY=3.00
PRIMER_PAIR_172_COMPL_END=1.00
PRIMER_PAIR_172_PRODUCT_SIZE=540
PRIMER_PAIR_172_PRODUCT_TM=79.9
PRIMER_PAIR_173_PENALTY=7.975020
PRIMER_LEFT_173_PENALTY=2.395059
PRIMER_RIGHT_173_PENALTY=5.579961
PRIMER_LEFT_173_SEQUENCE=TCAGGGACCAAAACCTGC
PRIMER_RIGHT_173_SEQUENCE=CTGCTGTTGTAGGAAGTTTAACAGG
PRIMER_LEFT_173=368,18
PRIMER_RIGHT_173=1304,25
PRIMER_LEFT_173_TM=59.605
PRIMER_RIGHT_173_TM=60.580
PRIMER_LEFT_173_GC_PERCENT=55.556
PRIMER_RIGHT_173_GC_PERCENT=44.000
PRIMER_LEFT_173_SELF_ANY=4.00
PRIMER_RIGHT_173_SELF_ANY=5.00
PRIMER_LEFT_173_SELF_END=2.00
PRIMER_RIGHT_173_SELF_END=1.00
PRIMER_LEFT_173_END_STABILITY=9.7000
PRIMER_RIGHT_173_END_STABILITY=7.9000
PRIMER_PAIR_173_COMPL_ANY=4.00
PRIMER_PAIR_173_COMPL_END=3.00
PRIMER_PAIR_173_PRODUCT_SIZE=937
PRIMER_PAIR_173_PRODUCT_TM=80.8
PRIMER_PAIR_174_PENALTY=8.015547
PRIMER_LEFT_174_PENALTY=3.939502
PRIMER_RIGHT_174_PENALTY=4.076046
PRIMER_LEFT_174_SEQUENCE=TGACTCGCCTGGCTCTGA
PRIMER_RIGHT_174_SEQUENCE=CAGGGTAGACTTCTTGGCAATACT
PRIMER_LEFT_174=8,18
PRIMER_RIGHT_174=434,24
PRIMER_LEFT_174_TM=61.940
PRIMER_RIGHT_174_TM=60.076
PRIMER_LEFT_174_GC_PERCENT=61.111
PRIMER_RIGHT_174_GC_PERCENT=45.833
PRIMER_LEFT_174_SELF_ANY=5.00
PRIMER_RIGHT_174_SELF_ANY=5.00
PRIMER_LEFT_174_SELF_END=3.00
PRIMER_RIGHT_174_SELF_END=2.00
PRIMER_LEFT_174_END_STABILITY=6.7000
PRIMER_RIGHT_174_END_STABILITY=5.3000
PRIMER_PAIR_174_COMPL_ANY=4.00
PRIMER_PAIR_174_COMPL_END=1.00
PRIMER_PAIR_174_PRODUCT_SIZE=427
PRIMER_PAIR_174_PRODUCT_TM=85.3
PRIMER_PAIR_175_PENALTY=8.072770
PRIMER_LEFT_175_PENALTY=2.420393
PRIMER_RIGHT_175_PENALTY=5.652377
PRIMER_LEFT_175_SEQUENCE=TGCCATGTTCTGTGGCAG
PRIMER_RIGHT_175_SEQUENCE=ACTCTTCTCACTGCATGTCTCTT
PRIMER_LEFT_175=301,18
PRIMER_RIGHT_175=685,23
PRIMER_LEFT_175_TM=60.420
PRIMER_RIGHT_175_TM=57.348
PRIMER_LEFT_175_GC_PERCENT=55.556
PRIMER_RIGHT_175_GC_PERCENT=43.478
PRIMER_LEFT_175_SELF_ANY=5.00
PRIMER_RIGHT_175_SELF_ANY=4.00
PRIMER_LEFT_175_SELF_END=3.00
PRIMER_RIGHT_175_SELF_END=0.00
PRIMER_LEFT_175_END_STABILITY=9.7000
PRIMER_RIGHT_175_END_STABILITY=6.7000
PRIMER_PAIR_175_COMPL_ANY=4.00
PRIMER_PAIR_175_COMPL_END=1.00
PRIMER_PAIR_175_PRODUCT_SIZE=385
PRIMER_PAIR_175_PRODUCT_TM=78.9
PRIMER_PAIR_176_PENALTY=8.127519
PRIMER_LEFT_176_PENALTY=2.423358
PRIMER_RIGHT_176_PENALTY=5.704162
PRIMER_LEFT_176_SEQUENCE=TCCAGAATGGGAAGTGGGA
PRIMER_RIGHT_176_SEQUENCE=CTTACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_176=339,19
PRIMER_RIGHT_176=570,25
PRIMER_LEFT_176_TM=61.423
PRIMER_RIGHT_176_TM=60.704
PRIMER_LEFT_176_GC_PERCENT=52.632
PRIMER_RIGHT_176_GC_PERCENT=44.000
PRIMER_LEFT_176_SELF_ANY=3.00
PRIMER_RIGHT_176_SELF_ANY=4.00
PRIMER_LEFT_176_SELF_END=0.00
PRIMER_RIGHT_176_SELF_END=3.00
PRIMER_LEFT_176_END_STABILITY=9.7000
PRIMER_RIGHT_176_END_STABILITY=9.7000
PRIMER_PAIR_176_COMPL_ANY=4.00
PRIMER_PAIR_176_COMPL_END=0.00
PRIMER_PAIR_176_PRODUCT_SIZE=232
PRIMER_PAIR_176_PRODUCT_TM=78.3
PRIMER_PAIR_177_PENALTY=8.138201
PRIMER_LEFT_177_PENALTY=7.723082
PRIMER_RIGHT_177_PENALTY=0.415119
PRIMER_LEFT_177_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_177_SEQUENCE=TGTCAAAGTTGTTCCGGTTG
PRIMER_LEFT_177=414,25
PRIMER_RIGHT_177=1196,20
PRIMER_LEFT_177_TM=62.723
PRIMER_RIGHT_177_TM=59.585
PRIMER_LEFT_177_GC_PERCENT=44.000
PRIMER_RIGHT_177_GC_PERCENT=45.000
PRIMER_LEFT_177_SELF_ANY=5.00
PRIMER_RIGHT_177_SELF_ANY=4.00
PRIMER_LEFT_177_SELF_END=1.00
PRIMER_RIGHT_177_SELF_END=0.00
PRIMER_LEFT_177_END_STABILITY=6.4000
PRIMER_RIGHT_177_END_STABILITY=8.2000
PRIMER_PAIR_177_COMPL_ANY=5.00
PRIMER_PAIR_177_COMPL_END=3.00
PRIMER_PAIR_177_PRODUCT_SIZE=783
PRIMER_PAIR_177_PRODUCT_TM=80.8
PRIMER_PAIR_178_PENALTY=8.265617
PRIMER_LEFT_178_PENALTY=2.561455
PRIMER_RIGHT_178_PENALTY=5.704162
PRIMER_LEFT_178_SEQUENCE=GCACATGAATGTCCAGAATG
PRIMER_RIGHT_178_SEQUENCE=GCTGCTGTTGTAGGAAGTTTAACAG
PRIMER_LEFT_178=328,20
PRIMER_RIGHT_178=1305,25
PRIMER_LEFT_178_TM=57.439
PRIMER_RIGHT_178_TM=60.704
PRIMER_LEFT_178_GC_PERCENT=45.000
PRIMER_RIGHT_178_GC_PERCENT=44.000
PRIMER_LEFT_178_SELF_ANY=6.00
PRIMER_RIGHT_178_SELF_ANY=5.00
PRIMER_LEFT_178_SELF_END=1.00
PRIMER_RIGHT_178_SELF_END=3.00
PRIMER_LEFT_178_END_STABILITY=6.9000
PRIMER_RIGHT_178_END_STABILITY=6.7000
PRIMER_PAIR_178_COMPL_ANY=3.00
PRIMER_PAIR_178_COMPL_END=1.00
PRIMER_PAIR_178_PRODUCT_SIZE=978
PRIMER_PAIR_178_PRODUCT_TM=80.8
PRIMER_PAIR_179_PENALTY=8.331209
PRIMER_LEFT_179_PENALTY=2.561455
PRIMER_RIGHT_179_PENALTY=5.769754
PRIMER_LEFT_179_SEQUENCE=TGCACATGAATGTCCAGAAT
PRIMER_RIGHT_179_SEQUENCE=GCTGTTGTAGGAAGTTTAACAGG
PRIMER_LEFT_179=327,20
PRIMER_RIGHT_179=1302,23
PRIMER_LEFT_179_TM=57.439
PRIMER_RIGHT_179_TM=57.230
PRIMER_LEFT_179_GC_PERCENT=40.000
PRIMER_RIGHT_179_GC_PERCENT=43.478
PRIMER_LEFT_179_SELF_ANY=8.00
PRIMER_RIGHT_179_SELF_ANY=5.00
PRIMER_LEFT_179_SELF_END=2.00
PRIMER_RIGHT_179_SELF_END=1.00
PRIMER_LEFT_179_END_STABILITY=6.6000
PRIMER_RIGHT_179_END_STABILITY=7.9000
PRIMER_PAIR_179_COMPL_ANY=3.00
PRIMER_PAIR_179_COMPL_END=0.00
PRIMER_PAIR_179_PRODUCT_SIZE=976
PRIMER_PAIR_179_PRODUCT_TM=80.8
PRIMER_PAIR_180_PENALTY=8.348945
PRIMER_LEFT_180_PENALTY=2.561455
PRIMER_RIGHT_180_PENALTY=5.787490
PRIMER_LEFT_180_SEQUENCE=ATGCACATGAATGTCCAGAA
PRIMER_RIGHT_180_SEQUENCE=GCTGTTGTAGGAAGTTTAACAGGAT
PRIMER_LEFT_180=326,20
PRIMER_RIGHT_180=1302,25
PRIMER_LEFT_180_TM=57.439
PRIMER_RIGHT_180_TM=59.213
PRIMER_LEFT_180_GC_PERCENT=40.000
PRIMER_RIGHT_180_GC_PERCENT=40.000
PRIMER_LEFT_180_SELF_ANY=8.00
PRIMER_RIGHT_180_SELF_ANY=5.00
PRIMER_LEFT_180_SELF_END=1.00
PRIMER_RIGHT_180_SELF_END=2.00
PRIMER_LEFT_180_END_STABILITY=7.0000
PRIMER_RIGHT_180_END_STABILITY=7.8000
PRIMER_PAIR_180_COMPL_ANY=3.00
PRIMER_PAIR_180_COMPL_END=2.00
PRIMER_PAIR_180_PRODUCT_SIZE=977
PRIMER_PAIR_180_PRODUCT_TM=80.7
PRIMER_PAIR_181_PENALTY=8.352989
PRIMER_LEFT_181_PENALTY=2.485003
PRIMER_RIGHT_181_PENALTY=5.867986
PRIMER_LEFT_181_SEQUENCE=TACCCACTGATGGTAATGCTG
PRIMER_RIGHT_181_SEQUENCE=ACAAACTCACCAACTAAGCAGCG
PRIMER_LEFT_181=258,21
PRIMER_RIGHT_181=567,23
PRIMER_LEFT_181_TM=58.515
PRIMER_RIGHT_181_TM=62.868
PRIMER_LEFT_181_GC_PERCENT=47.619
PRIMER_RIGHT_181_GC_PERCENT=47.826
PRIMER_LEFT_181_SELF_ANY=5.00
PRIMER_RIGHT_181_SELF_ANY=3.00
PRIMER_LEFT_181_SELF_END=1.00
PRIMER_RIGHT_181_SELF_END=2.00
PRIMER_LEFT_181_END_STABILITY=8.5000
PRIMER_RIGHT_181_END_STABILITY=10.2000
PRIMER_PAIR_181_COMPL_ANY=4.00
PRIMER_PAIR_181_COMPL_END=3.00
PRIMER_PAIR_181_PRODUCT_SIZE=310
PRIMER_PAIR_181_PRODUCT_TM=79.1
PRIMER_PAIR_182_PENALTY=8.417976
PRIMER_LEFT_182_PENALTY=4.091249
PRIMER_RIGHT_182_PENALTY=4.326727
PRIMER_LEFT_182_SEQUENCE=GATCCCACTCGCACAGCA
PRIMER_RIGHT_182_SEQUENCE=TTCAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_182=153,18
PRIMER_RIGHT_182=436,23
PRIMER_LEFT_182_TM=62.091
PRIMER_RIGHT_182_TM=61.327
PRIMER_LEFT_182_GC_PERCENT=61.111
PRIMER_RIGHT_182_GC_PERCENT=43.478
PRIMER_LEFT_182_SELF_ANY=4.00
PRIMER_RIGHT_182_SELF_ANY=5.00
PRIMER_LEFT_182_SELF_END=0.00
PRIMER_RIGHT_182_SELF_END=3.00
PRIMER_LEFT_182_END_STABILITY=8.5000
PRIMER_RIGHT_182_END_STABILITY=8.4000
PRIMER_PAIR_182_COMPL_ANY=3.00
PRIMER_PAIR_182_COMPL_END=1.00
PRIMER_PAIR_182_PRODUCT_SIZE=284
PRIMER_PAIR_182_PRODUCT_TM=81.8
PRIMER_PAIR_183_PENALTY=8.462996
PRIMER_LEFT_183_PENALTY=2.601441
PRIMER_RIGHT_183_PENALTY=5.861555
PRIMER_LEFT_183_SEQUENCE=GACCAAAACCTGCATTGATACC
PRIMER_RIGHT_183_SEQUENCE=TACTTTGTCTTCACTCCCATCTGC
PRIMER_LEFT_183=373,22
PRIMER_RIGHT_183=874,24
PRIMER_LEFT_183_TM=60.601
PRIMER_RIGHT_183_TM=61.862
PRIMER_LEFT_183_GC_PERCENT=45.455
PRIMER_RIGHT_183_GC_PERCENT=45.833
PRIMER_LEFT_183_SELF_ANY=4.00
PRIMER_RIGHT_183_SELF_ANY=2.00
PRIMER_LEFT_183_SELF_END=2.00
PRIMER_RIGHT_183_SELF_END=2.00
PRIMER_LEFT_183_END_STABILITY=6.8000
PRIMER_RIGHT_183_END_STABILITY=8.2000
PRIMER_PAIR_183_COMPL_ANY=4.00
PRIMER_PAIR_183_COMPL_END=0.00
PRIMER_PAIR_183_PRODUCT_SIZE=502
PRIMER_PAIR_183_PRODUCT_TM=79.7
PRIMER_PAIR_184_PENALTY=8.522458
PRIMER_LEFT_184_PENALTY=2.578834
PRIMER_RIGHT_184_PENALTY=5.943624
PRIMER_LEFT_184_SEQUENCE=AATGTCCAGAATGGGAAGTG
PRIMER_RIGHT_184_SEQUENCE=CTGGCTGCTGTTGTAGGAAGTTTA
PRIMER_LEFT_184=335,20
PRIMER_RIGHT_184=1308,24
PRIMER_LEFT_184_TM=57.421
PRIMER_RIGHT_184_TM=61.944
PRIMER_LEFT_184_GC_PERCENT=45.000
PRIMER_RIGHT_184_GC_PERCENT=45.833
PRIMER_LEFT_184_SELF_ANY=3.00
PRIMER_RIGHT_184_SELF_ANY=3.00
PRIMER_LEFT_184_SELF_END=0.00
PRIMER_RIGHT_184_SELF_END=2.00
PRIMER_LEFT_184_END_STABILITY=6.7000
PRIMER_RIGHT_184_END_STABILITY=6.0000
PRIMER_PAIR_184_COMPL_ANY=4.00
PRIMER_PAIR_184_COMPL_END=0.00
PRIMER_PAIR_184_PRODUCT_SIZE=974
PRIMER_PAIR_184_PRODUCT_TM=80.8
PRIMER_PAIR_185_PENALTY=8.617094
PRIMER_LEFT_185_PENALTY=2.601441
PRIMER_RIGHT_185_PENALTY=6.015654
PRIMER_LEFT_185_SEQUENCE=GGACCAAAACCTGCATTGATAC
PRIMER_RIGHT_185_SEQUENCE=TCACTTACAAACTCACCAACTAAGC
PRIMER_LEFT_185=372,22
PRIMER_RIGHT_185=573,25
PRIMER_LEFT_185_TM=60.601
PRIMER_RIGHT_185_TM=58.984
PRIMER_LEFT_185_GC_PERCENT=45.455
PRIMER_RIGHT_185_GC_PERCENT=40.000
PRIMER_LEFT_185_SELF_ANY=4.00
PRIMER_RIGHT_185_SELF_ANY=4.00
PRIMER_LEFT_185_SELF_END=2.00
PRIMER_RIGHT_185_SELF_END=2.00
PRIMER_LEFT_185_END_STABILITY=5.3000
PRIMER_RIGHT_185_END_STABILITY=7.5000
PRIMER_PAIR_185_COMPL_ANY=3.00
PRIMER_PAIR_185_COMPL_END=0.00
PRIMER_PAIR_185_PRODUCT_SIZE=202
PRIMER_PAIR_185_PRODUCT_TM=78.0
PRIMER_PAIR_186_PENALTY=8.720488
PRIMER_LEFT_186_PENALTY=2.612614
PRIMER_RIGHT_186_PENALTY=6.107874
PRIMER_LEFT_186_SEQUENCE=CCATCAGGGACCAAAACCTG
PRIMER_RIGHT_186_SEQUENCE=TACTACTTTGTCTTCACTCCCATCTG
PRIMER_LEFT_186=365,20
PRIMER_RIGHT_186=877,26
PRIMER_LEFT_186_TM=62.613
PRIMER_RIGHT_186_TM=60.108
PRIMER_LEFT_186_GC_PERCENT=55.000
PRIMER_RIGHT_186_GC_PERCENT=42.308
PRIMER_LEFT_186_SELF_ANY=4.00
PRIMER_RIGHT_186_SELF_ANY=3.00
PRIMER_LEFT_186_SELF_END=1.00
PRIMER_RIGHT_186_SELF_END=2.00
PRIMER_LEFT_186_END_STABILITY=7.9000
PRIMER_RIGHT_186_END_STABILITY=6.6000
PRIMER_PAIR_186_COMPL_ANY=4.00
PRIMER_PAIR_186_COMPL_END=1.00
PRIMER_PAIR_186_PRODUCT_SIZE=513
PRIMER_PAIR_186_PRODUCT_TM=79.8
PRIMER_PAIR_187_PENALTY=8.809127
PRIMER_LEFT_187_PENALTY=2.606855
PRIMER_RIGHT_187_PENALTY=6.202271
PRIMER_LEFT_187_SEQUENCE=CCAGAATGGGAAGTGGGA
PRIMER_RIGHT_187_SEQUENCE=TACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_187=340,18
PRIMER_RIGHT_187=686,25
PRIMER_LEFT_187_TM=59.393
PRIMER_RIGHT_187_TM=58.798
PRIMER_LEFT_187_GC_PERCENT=55.556
PRIMER_RIGHT_187_GC_PERCENT=40.000
PRIMER_LEFT_187_SELF_ANY=3.00
PRIMER_RIGHT_187_SELF_ANY=4.00
PRIMER_LEFT_187_SELF_END=0.00
PRIMER_RIGHT_187_SELF_END=0.00
PRIMER_LEFT_187_END_STABILITY=9.7000
PRIMER_RIGHT_187_END_STABILITY=7.0000
PRIMER_PAIR_187_COMPL_ANY=5.00
PRIMER_PAIR_187_COMPL_END=2.00
PRIMER_PAIR_187_PRODUCT_SIZE=347
PRIMER_PAIR_187_PRODUCT_TM=78.9
PRIMER_PAIR_188_PENALTY=8.875619
PRIMER_LEFT_188_PENALTY=4.361392
PRIMER_RIGHT_188_PENALTY=4.514227
PRIMER_LEFT_188_SEQUENCE=TCCGTCAGTTTCCTCGGC
PRIMER_RIGHT_188_SEQUENCE=AGTTCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_188=47,18
PRIMER_RIGHT_188=438,23
PRIMER_LEFT_188_TM=62.361
PRIMER_RIGHT_188_TM=61.514
PRIMER_LEFT_188_GC_PERCENT=61.111
PRIMER_RIGHT_188_GC_PERCENT=47.826
PRIMER_LEFT_188_SELF_ANY=3.00
PRIMER_RIGHT_188_SELF_ANY=4.00
PRIMER_LEFT_188_SELF_END=2.00
PRIMER_RIGHT_188_SELF_END=3.00
PRIMER_LEFT_188_END_STABILITY=11.4000
PRIMER_RIGHT_188_END_STABILITY=10.0000
PRIMER_PAIR_188_COMPL_ANY=3.00
PRIMER_PAIR_188_COMPL_END=1.00
PRIMER_PAIR_188_PRODUCT_SIZE=392
PRIMER_PAIR_188_PRODUCT_TM=84.4
PRIMER_PAIR_189_PENALTY=8.875619
PRIMER_LEFT_189_PENALTY=4.361392
PRIMER_RIGHT_189_PENALTY=4.514227
PRIMER_LEFT_189_SEQUENCE=TCGGGCTCCGTCAGTTTC
PRIMER_RIGHT_189_SEQUENCE=CAGTTCAGGGTAGACTTCTTGGC
PRIMER_LEFT_189=41,18
PRIMER_RIGHT_189=439,23
PRIMER_LEFT_189_TM=62.361
PRIMER_RIGHT_189_TM=61.514
PRIMER_LEFT_189_GC_PERCENT=61.111
PRIMER_RIGHT_189_GC_PERCENT=52.174
PRIMER_LEFT_189_SELF_ANY=4.00
PRIMER_RIGHT_189_SELF_ANY=4.00
PRIMER_LEFT_189_SELF_END=0.00
PRIMER_RIGHT_189_SELF_END=2.00
PRIMER_LEFT_189_END_STABILITY=6.7000
PRIMER_RIGHT_189_END_STABILITY=10.0000
PRIMER_PAIR_189_COMPL_ANY=3.00
PRIMER_PAIR_189_COMPL_END=0.00
PRIMER_PAIR_189_PRODUCT_SIZE=399
PRIMER_PAIR_189_PRODUCT_TM=84.6
PRIMER_PAIR_190_PENALTY=8.888679
PRIMER_LEFT_190_PENALTY=2.613581
PRIMER_RIGHT_190_PENALTY=6.275098
PRIMER_LEFT_190_SEQUENCE=CTGGCTGAACCCCAGATTG
PRIMER_RIGHT_190_SEQUENCE=TGTCTTCACTCCCATCTGCATAGT
PRIMER_LEFT_190=284,19
PRIMER_RIGHT_190=869,24
PRIMER_LEFT_190_TM=61.614
PRIMER_RIGHT_190_TM=62.275
PRIMER_LEFT_190_GC_PERCENT=57.895
PRIMER_RIGHT_190_GC_PERCENT=45.833
PRIMER_LEFT_190_SELF_ANY=4.00
PRIMER_RIGHT_190_SELF_ANY=4.00
PRIMER_LEFT_190_SELF_END=2.00
PRIMER_RIGHT_190_SELF_END=2.00
PRIMER_LEFT_190_END_STABILITY=6.9000
PRIMER_RIGHT_190_END_STABILITY=5.3000
PRIMER_PAIR_190_COMPL_ANY=5.00
PRIMER_PAIR_190_COMPL_END=1.00
PRIMER_PAIR_190_PRODUCT_SIZE=586
PRIMER_PAIR_190_PRODUCT_TM=79.9
PRIMER_PAIR_191_PENALTY=8.911303
PRIMER_LEFT_191_PENALTY=4.397076
PRIMER_RIGHT_191_PENALTY=4.514227
PRIMER_LEFT_191_SEQUENCE=CGGTTTGGCACTGCTCCT
PRIMER_RIGHT_191_SEQUENCE=GCAGTTCAGGGTAGACTTCTTGG
PRIMER_LEFT_191=208,18
PRIMER_RIGHT_191=440,23
PRIMER_LEFT_191_TM=62.397
PRIMER_RIGHT_191_TM=61.514
PRIMER_LEFT_191_GC_PERCENT=61.111
PRIMER_RIGHT_191_GC_PERCENT=52.174
PRIMER_LEFT_191_SELF_ANY=5.00
PRIMER_RIGHT_191_SELF_ANY=4.00
PRIMER_LEFT_191_SELF_END=0.00
PRIMER_RIGHT_191_SELF_END=0.00
PRIMER_LEFT_191_END_STABILITY=7.9000
PRIMER_RIGHT_191_END_STABILITY=8.5000
PRIMER_PAIR_191_COMPL_ANY=5.00
PRIMER_PAIR_191_COMPL_END=1.00
PRIMER_PAIR_191_PRODUCT_SIZE=233
PRIMER_PAIR_191_PRODUCT_TM=80.0
PRIMER_PAIR_192_PENALTY=8.943335
PRIMER_LEFT_192_PENALTY=2.606855
PRIMER_RIGHT_192_PENALTY=6.336480
PRIMER_LEFT_192_SEQUENCE=TCCAGAATGGGAAGTGGG
PRIMER_RIGHT_192_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_192=339,18
PRIMER_RIGHT_192=685,25
PRIMER_LEFT_192_TM=59.393
PRIMER_RIGHT_192_TM=61.336
PRIMER_LEFT_192_GC_PERCENT=55.556
PRIMER_RIGHT_192_GC_PERCENT=44.000
PRIMER_LEFT_192_SELF_ANY=3.00
PRIMER_RIGHT_192_SELF_ANY=4.00
PRIMER_LEFT_192_SELF_END=0.00
PRIMER_RIGHT_192_SELF_END=0.00
PRIMER_LEFT_192_END_STABILITY=9.4000
PRIMER_RIGHT_192_END_STABILITY=7.3000
PRIMER_PAIR_192_COMPL_ANY=5.00
PRIMER_PAIR_192_COMPL_END=1.00
PRIMER_PAIR_192_PRODUCT_SIZE=347
PRIMER_PAIR_192_PRODUCT_TM=78.9
PRIMER_PAIR_193_PENALTY=8.974531
PRIMER_LEFT_193_PENALTY=8.555845
PRIMER_RIGHT_193_PENALTY=0.418685
PRIMER_LEFT_193_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_193_SEQUENCE=CGGCCATGCAGTACTCTTCT
PRIMER_LEFT_193=412,26
PRIMER_RIGHT_193=1217,20
PRIMER_LEFT_193_TM=62.556
PRIMER_RIGHT_193_TM=60.419
PRIMER_LEFT_193_GC_PERCENT=46.154
PRIMER_RIGHT_193_GC_PERCENT=55.000
PRIMER_LEFT_193_SELF_ANY=5.00
PRIMER_RIGHT_193_SELF_ANY=6.00
PRIMER_LEFT_193_SELF_END=0.00
PRIMER_RIGHT_193_SELF_END=1.00
PRIMER_LEFT_193_END_STABILITY=6.7000
PRIMER_RIGHT_193_END_STABILITY=6.7000
PRIMER_PAIR_193_COMPL_ANY=6.00
PRIMER_PAIR_193_COMPL_END=3.00
PRIMER_PAIR_193_PRODUCT_SIZE=806
PRIMER_PAIR_193_PRODUCT_TM=80.9
PRIMER_PAIR_194_PENALTY=8.981355
PRIMER_LEFT_194_PENALTY=2.631568
PRIMER_RIGHT_194_PENALTY=6.349786
PRIMER_LEFT_194_SEQUENCE=ATCCTGCAGTATTGCCAAGAAG
PRIMER_RIGHT_194_SEQUENCE=TGCTGTTGTAGGAAGTTTAACAGGA
PRIMER_LEFT_194=404,22
PRIMER_RIGHT_194=1303,25
PRIMER_LEFT_194_TM=60.632
PRIMER_RIGHT_194_TM=61.350
PRIMER_LEFT_194_GC_PERCENT=45.455
PRIMER_RIGHT_194_GC_PERCENT=40.000
PRIMER_LEFT_194_SELF_ANY=8.00
PRIMER_RIGHT_194_SELF_ANY=5.00
PRIMER_LEFT_194_SELF_END=2.00
PRIMER_RIGHT_194_SELF_END=3.00
PRIMER_LEFT_194_END_STABILITY=6.7000
PRIMER_RIGHT_194_END_STABILITY=8.2000
PRIMER_PAIR_194_COMPL_ANY=5.00
PRIMER_PAIR_194_COMPL_END=3.00
PRIMER_PAIR_194_PRODUCT_SIZE=900
PRIMER_PAIR_194_PRODUCT_TM=80.8
PRIMER_PAIR_195_PENALTY=9.037893
PRIMER_LEFT_195_PENALTY=2.617395
PRIMER_RIGHT_195_PENALTY=6.420498
PRIMER_LEFT_195_SEQUENCE=GTACCCACTGATGGTAATGCTG
PRIMER_RIGHT_195_SEQUENCE=TTCTACTACTTTGTCTTCACTCCCA
PRIMER_LEFT_195=257,22
PRIMER_RIGHT_195=880,25
PRIMER_LEFT_195_TM=59.383
PRIMER_RIGHT_195_TM=58.580
PRIMER_LEFT_195_GC_PERCENT=50.000
PRIMER_RIGHT_195_GC_PERCENT=40.000
PRIMER_LEFT_195_SELF_ANY=5.00
PRIMER_RIGHT_195_SELF_ANY=3.00
PRIMER_LEFT_195_SELF_END=1.00
PRIMER_RIGHT_195_SELF_END=0.00
PRIMER_LEFT_195_END_STABILITY=8.5000
PRIMER_RIGHT_195_END_STABILITY=9.7000
PRIMER_PAIR_195_COMPL_ANY=4.00
PRIMER_PAIR_195_COMPL_END=3.00
PRIMER_PAIR_195_PRODUCT_SIZE=624
PRIMER_PAIR_195_PRODUCT_TM=79.9
PRIMER_PAIR_196_PENALTY=9.052346
PRIMER_LEFT_196_PENALTY=2.618102
PRIMER_RIGHT_196_PENALTY=6.434244
PRIMER_LEFT_196_SEQUENCE=CTGAACCCCAGATTGCCA
PRIMER_RIGHT_196_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_196=288,18
PRIMER_RIGHT_196=687,26
PRIMER_LEFT_196_TM=60.618
PRIMER_RIGHT_196_TM=59.566
PRIMER_LEFT_196_GC_PERCENT=55.556
PRIMER_RIGHT_196_GC_PERCENT=42.308
PRIMER_LEFT_196_SELF_ANY=3.00
PRIMER_RIGHT_196_SELF_ANY=4.00
PRIMER_LEFT_196_SELF_END=3.00
PRIMER_RIGHT_196_SELF_END=0.00
PRIMER_LEFT_196_END_STABILITY=10.0000
PRIMER_RIGHT_196_END_STABILITY=7.0000
PRIMER_PAIR_196_COMPL_ANY=3.00
PRIMER_PAIR_196_COMPL_END=1.00
PRIMER_PAIR_196_PRODUCT_SIZE=400
PRIMER_PAIR_196_PRODUCT_TM=79.0
PRIMER_PAIR_197_PENALTY=9.095316
PRIMER_LEFT_197_PENALTY=2.644351
PRIMER_RIGHT_197_PENALTY=6.450965
PRIMER_LEFT_197_SEQUENCE=ATTCAGATCCATCAGGGACCA
PRIMER_RIGHT_197_SEQUENCE=ACTACTTTGTCTTCACTCCCATCT
PRIMER_LEFT_197=357,21
PRIMER_RIGHT_197=876,24
PRIMER_LEFT_197_TM=61.644
PRIMER_RIGHT_197_TM=57.549
PRIMER_LEFT_197_GC_PERCENT=47.619
PRIMER_RIGHT_197_GC_PERCENT=41.667
PRIMER_LEFT_197_SELF_ANY=5.00
PRIMER_RIGHT_197_SELF_ANY=2.00
PRIMER_LEFT_197_SELF_END=3.00
PRIMER_RIGHT_197_SELF_END=0.00
PRIMER_LEFT_197_END_STABILITY=7.9000
PRIMER_RIGHT_197_END_STABILITY=6.6000
PRIMER_PAIR_197_COMPL_ANY=4.00
PRIMER_PAIR_197_COMPL_END=1.00
PRIMER_PAIR_197_PRODUCT_SIZE=520
PRIMER_PAIR_197_PRODUCT_TM=79.6
PRIMER_PAIR_198_PENALTY=9.096848
PRIMER_LEFT_198_PENALTY=8.647019
PRIMER_RIGHT_198_PENALTY=0.449829
PRIMER_LEFT_198_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_198_SEQUENCE=TCGGCTTCTTCTTCTTCCAC
PRIMER_LEFT_198=413,26
PRIMER_RIGHT_198=927,20
PRIMER_LEFT_198_TM=62.647
PRIMER_RIGHT_198_TM=59.550
PRIMER_LEFT_198_GC_PERCENT=42.308
PRIMER_RIGHT_198_GC_PERCENT=50.000
PRIMER_LEFT_198_SELF_ANY=5.00
PRIMER_RIGHT_198_SELF_ANY=2.00
PRIMER_LEFT_198_SELF_END=1.00
PRIMER_RIGHT_198_SELF_END=0.00
PRIMER_LEFT_198_END_STABILITY=6.4000
PRIMER_RIGHT_198_END_STABILITY=7.9000
PRIMER_PAIR_198_COMPL_ANY=6.00
PRIMER_PAIR_198_COMPL_END=2.00
PRIMER_PAIR_198_PRODUCT_SIZE=515
PRIMER_PAIR_198_PRODUCT_TM=79.8
PRIMER_PAIR_199_PENALTY=9.102169
PRIMER_LEFT_199_PENALTY=8.647019
PRIMER_RIGHT_199_PENALTY=0.455150
PRIMER_LEFT_199_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_199_SEQUENCE=CCTCTCTTTGGCTTTCTGGA
PRIMER_LEFT_199=411,26
PRIMER_RIGHT_199=1390,20
PRIMER_LEFT_199_TM=62.647
PRIMER_RIGHT_199_TM=59.545
PRIMER_LEFT_199_GC_PERCENT=42.308
PRIMER_RIGHT_199_GC_PERCENT=50.000
PRIMER_LEFT_199_SELF_ANY=5.00
PRIMER_RIGHT_199_SELF_ANY=2.00
PRIMER_LEFT_199_SELF_END=0.00
PRIMER_RIGHT_199_SELF_END=2.00
PRIMER_LEFT_199_END_STABILITY=7.0000
PRIMER_RIGHT_199_END_STABILITY=8.2000
PRIMER_PAIR_199_COMPL_ANY=5.00
PRIMER_PAIR_199_COMPL_END=3.00
PRIMER_PAIR_199_PRODUCT_SIZE=980
PRIMER_PAIR_199_PRODUCT_TM=80.8
=
SEQUENCE_ID=ok_regions_test2
[encoded data, 10786 characters]
PRIMER_NUM_RETURN=100
PRIMER_PRODUCT_SIZE_RANGE=75-800
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,251,,;,,1,251;
PRIMER_MIN_THREE_PRIME_DISTANCE=0
PRIMER_LEFT_EXPLAIN=considered 106940, GC content failed 617, low tm 78, high tm 1421, high end compl 39, not in any ok left region 104635, ok 150
PRIMER_RIGHT_EXPLAIN=considered 106876, GC content failed 647, low tm 334, high tm 7627, high any compl 3, high end compl 29, long poly-x seq 10, not in any ok right region 97330, ok 896
PRIMER_PAIR_EXPLAIN=considered 1174, unacceptable product size 987, high any compl 1, high end compl 56, primer in pair overlaps a primer in a better pair 39258, ok 130
PRIMER_LEFT_NUM_RETURNED=100
PRIMER_RIGHT_NUM_RETURNED=100
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=100
PRIMER_PAIR_0_PENALTY=0.085464
PRIMER_LEFT_0_PENALTY=0.042732
PRIMER_RIGHT_0_PENALTY=0.042732
PRIMER_LEFT_0_SEQUENCE=GAGAGCCTGAGTTGGCATTC
PRIMER_RIGHT_0_SEQUENCE=GGAACTTGATCCAGCAGAGC
PRIMER_LEFT_0=188,20
PRIMER_RIGHT_0=754,20
PRIMER_LEFT_0_TM=59.957
PRIMER_RIGHT_0_TM=59.957
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=567
PRIMER_PAIR_0_PRODUCT_TM=84.4
PRIMER_PAIR_1_PENALTY=0.489932
PRIMER_LEFT_1_PENALTY=0.394220
PRIMER_RIGHT_1_PENALTY=0.095713
PRIMER_LEFT_1_SEQUENCE=CAAGGAGATGGGATGGAGAG
PRIMER_RIGHT_1_SEQUENCE=AGGGAGCGGTAGAAGTGGAT
PRIMER_LEFT_1=173,20
PRIMER_RIGHT_1=899,20
PRIMER_LEFT_1_TM=59.606
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=727
PRIMER_PAIR_1_PRODUCT_TM=85.1
PRIMER_PAIR_2_PENALTY=0.696500
PRIMER_LEFT_2_PENALTY=0.562760
PRIMER_RIGHT_2_PENALTY=0.133741
PRIMER_LEFT_2_SEQUENCE=AAGGAGATGGGATGGAGAGC
PRIMER_RIGHT_2_SEQUENCE=AGCGGTAGAAGTGGATGGTG
PRIMER_LEFT_2=174,20
PRIMER_RIGHT_2=895,20
PRIMER_LEFT_2_TM=60.563
PRIMER_RIGHT_2_TM=60.134
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=722
PRIMER_PAIR_2_PRODUCT_TM=85.0
PRIMER_PAIR_3_PENALTY=0.963400
PRIMER_LEFT_3_PENALTY=0.796337
PRIMER_RIGHT_3_PENALTY=0.167063
PRIMER_LEFT_3_SEQUENCE=AGCCTGAGTTGGCATTCGTA
PRIMER_RIGHT_3_SEQUENCE=CGGTAATCCATGCCCTTATG
PRIMER_LEFT_3=191,20
PRIMER_RIGHT_3=314,20
PRIMER_LEFT_3_TM=60.796
PRIMER_RIGHT_3_TM=60.167
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=124
PRIMER_PAIR_3_PRODUCT_TM=78.9
PRIMER_PAIR_4_PENALTY=1.036721
PRIMER_LEFT_4_PENALTY=0.846380
PRIMER_RIGHT_4_PENALTY=0.190341
PRIMER_LEFT_4_SEQUENCE=ATAAATGACCTGCCTGGCTC
PRIMER_RIGHT_4_SEQUENCE=GAAGACCACGAAGATGAGGC
PRIMER_LEFT_4=210,20
PRIMER_RIGHT_4=876,20
PRIMER_LEFT_4_TM=59.154
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=667
PRIMER_PAIR_4_PRODUCT_TM=85.3
PRIMER_PAIR_5_PENALTY=1.216058
PRIMER_LEFT_5_PENALTY=1.022938
PRIMER_RIGHT_5_PENALTY=0.193120
PRIMER_LEFT_5_SEQUENCE=ATGGGATGGAGAGCCTGAGT
PRIMER_RIGHT_5_SEQUENCE=CGGGTACTGGTACTGCGTCT
PRIMER_LEFT_5=180,20
PRIMER_RIGHT_5=504,20
PRIMER_LEFT_5_TM=61.023
PRIMER_RIGHT_5_TM=60.193
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=60.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=325
PRIMER_PAIR_5_PRODUCT_TM=83.4
PRIMER_PAIR_6_PENALTY=1.242484
PRIMER_LEFT_6_PENALTY=1.032614
PRIMER_RIGHT_6_PENALTY=0.209870
PRIMER_LEFT_6_SEQUENCE=TCGTATAAATGACCTGCCTGG
PRIMER_RIGHT_6_SEQUENCE=CCAGGAAGAGTAGGATGCCA
PRIMER_LEFT_6=206,21
PRIMER_RIGHT_6=721,20
PRIMER_LEFT_6_TM=59.967
PRIMER_RIGHT_6_TM=60.210
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=10.0000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=516
PRIMER_PAIR_6_PRODUCT_TM=84.5
PRIMER_PAIR_7_PENALTY=1.364928
PRIMER_LEFT_7_PENALTY=1.168025
PRIMER_RIGHT_7_PENALTY=0.196903
PRIMER_LEFT_7_SEQUENCE=ATGGGATGGAGAGCCTGAG
PRIMER_RIGHT_7_SEQUENCE=GGAGGAACTTGATCCAGCAG
PRIMER_LEFT_7=180,19
PRIMER_RIGHT_7=757,20
PRIMER_LEFT_7_TM=60.168
PRIMER_RIGHT_7_TM=59.803
PRIMER_LEFT_7_GC_PERCENT=57.895
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=578
PRIMER_PAIR_7_PRODUCT_TM=84.4
PRIMER_PAIR_8_PENALTY=1.392367
PRIMER_LEFT_8_PENALTY=1.168025
PRIMER_RIGHT_8_PENALTY=0.224341
PRIMER_LEFT_8_SEQUENCE=AGATGGGATGGAGAGCCTG
PRIMER_RIGHT_8_SEQUENCE=TAGGCACGTTAAGCTCAGCA
PRIMER_LEFT_8=178,19
PRIMER_RIGHT_8=259,20
PRIMER_LEFT_8_TM=60.168
PRIMER_RIGHT_8_TM=59.776
PRIMER_LEFT_8_GC_PERCENT=57.895
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=82
PRIMER_PAIR_8_PRODUCT_TM=74.1
PRIMER_PAIR_9_PENALTY=1.476386
PRIMER_LEFT_9_PENALTY=1.185797
PRIMER_RIGHT_9_PENALTY=0.290589
PRIMER_LEFT_9_SEQUENCE=GCCTGAGTTGGCATTCGTA
PRIMER_RIGHT_9_SEQUENCE=CTGCTCAGGTAGAGCTTCCG
PRIMER_LEFT_9=192,19
PRIMER_RIGHT_9=404,20
PRIMER_LEFT_9_TM=59.814
PRIMER_RIGHT_9_TM=60.291
PRIMER_LEFT_9_GC_PERCENT=52.632
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_END_STABILITY=7.4000
PRIMER_RIGHT_9_END_STABILITY=10.2000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=213
PRIMER_PAIR_9_PRODUCT_TM=81.9
PRIMER_PAIR_10_PENALTY=1.629195
PRIMER_LEFT_10_PENALTY=1.365052
PRIMER_RIGHT_10_PENALTY=0.264143
PRIMER_LEFT_10_SEQUENCE=GGAGAGCCTGAGTTGGCAT
PRIMER_RIGHT_10_SEQUENCE=GGTCGATAGGCACGTTAAGC
PRIMER_LEFT_10=187,19
PRIMER_RIGHT_10=265,20
PRIMER_LEFT_10_TM=60.365
PRIMER_RIGHT_10_TM=59.736
PRIMER_LEFT_10_GC_PERCENT=57.895
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=7.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=79
PRIMER_PAIR_10_PRODUCT_TM=74.6
PRIMER_PAIR_11_PENALTY=1.649114
PRIMER_LEFT_11_PENALTY=1.606382
PRIMER_RIGHT_11_PENALTY=0.042732
PRIMER_LEFT_11_SEQUENCE=AGGGATGGAAGTGCTTGGA
PRIMER_RIGHT_11_SEQUENCE=GAATGCCAACTCAGGCTCTC
PRIMER_LEFT_11=127,19
PRIMER_RIGHT_11=207,20
PRIMER_LEFT_11_TM=60.606
PRIMER_RIGHT_11_TM=59.957
PRIMER_LEFT_11_GC_PERCENT=52.632
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=81
PRIMER_PAIR_11_PRODUCT_TM=76.8
PRIMER_PAIR_12_PENALTY=1.682987
PRIMER_LEFT_12_PENALTY=1.365052
PRIMER_RIGHT_12_PENALTY=0.317935
PRIMER_LEFT_12_SEQUENCE=ATGGAGAGCCTGAGTTGGC
PRIMER_RIGHT_12_SEQUENCE=TCAGGTTGTGGATGTTGCTC
PRIMER_LEFT_12=185,19
PRIMER_RIGHT_12=628,20
PRIMER_LEFT_12_TM=60.365
PRIMER_RIGHT_12_TM=59.682
PRIMER_LEFT_12_GC_PERCENT=57.895
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=444
PRIMER_PAIR_12_PRODUCT_TM=84.1
PRIMER_PAIR_13_PENALTY=1.747525
PRIMER_LEFT_13_PENALTY=1.423250
PRIMER_RIGHT_13_PENALTY=0.324275
PRIMER_LEFT_13_SEQUENCE=CCACCATGAGTGCTGAGCTT
PRIMER_RIGHT_13_SEQUENCE=TGGTGAAGACCACGAAGATG
PRIMER_LEFT_13=230,20
PRIMER_RIGHT_13=880,20
PRIMER_LEFT_13_TM=61.423
PRIMER_RIGHT_13_TM=59.676
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=651
PRIMER_PAIR_13_PRODUCT_TM=85.4
PRIMER_PAIR_14_PENALTY=1.917502
PRIMER_LEFT_14_PENALTY=1.814316
PRIMER_RIGHT_14_PENALTY=0.103187
PRIMER_LEFT_14_SEQUENCE=GATGGAAGTGCTTGGATGC
PRIMER_RIGHT_14_SEQUENCE=ATACGAATGCCAACTCAGGC
PRIMER_LEFT_14=130,19
PRIMER_RIGHT_14=211,20
PRIMER_LEFT_14_TM=59.186
PRIMER_RIGHT_14_TM=60.103
PRIMER_LEFT_14_GC_PERCENT=52.632
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=82
PRIMER_PAIR_14_PRODUCT_TM=76.1
PRIMER_PAIR_15_PENALTY=1.979863
PRIMER_LEFT_15_PENALTY=1.571682
PRIMER_RIGHT_15_PENALTY=0.408180
PRIMER_LEFT_15_SEQUENCE=GCAAGGAGATGGGATGGAG
PRIMER_RIGHT_15_SEQUENCE=GGGTCGATAGGCACGTTAAG
PRIMER_LEFT_15=172,19
PRIMER_RIGHT_15=266,20
PRIMER_LEFT_15_TM=60.572
PRIMER_RIGHT_15_TM=59.592
PRIMER_LEFT_15_GC_PERCENT=57.895
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.3000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=95
PRIMER_PAIR_15_PRODUCT_TM=76.0
PRIMER_PAIR_16_PENALTY=2.110563
PRIMER_LEFT_16_PENALTY=1.702162
PRIMER_RIGHT_16_PENALTY=0.408401
PRIMER_LEFT_16_SEQUENCE=GGAGAGCCTGAGTTGGCATT
PRIMER_RIGHT_16_SEQUENCE=CTCGCTGATGGAGTTCAGGT
PRIMER_LEFT_16=187,20
PRIMER_RIGHT_16=642,20
PRIMER_LEFT_16_TM=61.702
PRIMER_RIGHT_16_TM=60.408
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=456
PRIMER_PAIR_16_PRODUCT_TM=84.2
PRIMER_PAIR_17_PENALTY=2.186862
PRIMER_LEFT_17_PENALTY=1.778461
PRIMER_RIGHT_17_PENALTY=0.408401
PRIMER_LEFT_17_SEQUENCE=TGGGATGGAGAGCCTGAGT
PRIMER_RIGHT_17_SEQUENCE=ACTCGCTGATGGAGTTCAGG
PRIMER_LEFT_17=181,19
PRIMER_RIGHT_17=643,20
PRIMER_LEFT_17_TM=60.778
PRIMER_RIGHT_17_TM=60.408
PRIMER_LEFT_17_GC_PERCENT=57.895
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=463
PRIMER_PAIR_17_PRODUCT_TM=84.1
PRIMER_PAIR_18_PENALTY=2.223373
PRIMER_LEFT_18_PENALTY=1.804687
PRIMER_RIGHT_18_PENALTY=0.418685
PRIMER_LEFT_18_SEQUENCE=GGGATGGAGAGCCTGAGTT
PRIMER_RIGHT_18_SEQUENCE=AGAGTAGGATGCCAAGCACG
PRIMER_LEFT_18=182,19
PRIMER_RIGHT_18=715,20
PRIMER_LEFT_18_TM=59.195
PRIMER_RIGHT_18_TM=60.419
PRIMER_LEFT_18_GC_PERCENT=57.895
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=534
PRIMER_PAIR_18_PRODUCT_TM=84.4
PRIMER_PAIR_19_PENALTY=2.237985
PRIMER_LEFT_19_PENALTY=1.817214
PRIMER_RIGHT_19_PENALTY=0.420772
PRIMER_LEFT_19_SEQUENCE=AGCCTGAGTTGGCATTCGT
PRIMER_RIGHT_19_SEQUENCE=GGTGACCAGTTCCAGGTAGC
PRIMER_LEFT_19=191,19
PRIMER_RIGHT_19=351,20
PRIMER_LEFT_19_TM=60.817
PRIMER_RIGHT_19_TM=59.579
PRIMER_LEFT_19_GC_PERCENT=52.632
PRIMER_RIGHT_19_GC_PERCENT=60.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=161
PRIMER_PAIR_19_PRODUCT_TM=80.9
PRIMER_PAIR_20_PENALTY=2.249787
PRIMER_LEFT_20_PENALTY=1.804465
PRIMER_RIGHT_20_PENALTY=0.445321
PRIMER_LEFT_20_SEQUENCE=TATAAATGACCTGCCTGGCTC
PRIMER_RIGHT_20_SEQUENCE=CTTGTGGAGCTCCTCGATCT
PRIMER_LEFT_20=209,21
PRIMER_RIGHT_20=951,20
PRIMER_LEFT_20_TM=59.196
PRIMER_RIGHT_20_TM=59.555
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=8.00
PRIMER_LEFT_20_SELF_END=3.00
PRIMER_RIGHT_20_SELF_END=3.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.3000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=743
PRIMER_PAIR_20_PRODUCT_TM=85.3
PRIMER_PAIR_21_PENALTY=2.333688
PRIMER_LEFT_21_PENALTY=1.853206
PRIMER_RIGHT_21_PENALTY=0.480482
PRIMER_LEFT_21_SEQUENCE=TCCCACCATGAGTGCTGAG
PRIMER_RIGHT_21_SEQUENCE=TGGAGTTCAGGTTGTGGATG
PRIMER_LEFT_21=228,19
PRIMER_RIGHT_21=634,20
PRIMER_LEFT_21_TM=60.853
PRIMER_RIGHT_21_TM=59.520
PRIMER_LEFT_21_GC_PERCENT=57.895
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=3.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=407
PRIMER_PAIR_21_PRODUCT_TM=84.4
PRIMER_PAIR_22_PENALTY=2.333702
PRIMER_LEFT_22_PENALTY=1.853220
PRIMER_RIGHT_22_PENALTY=0.480482
PRIMER_LEFT_22_SEQUENCE=GAGATGGGATGGAGAGCCT
PRIMER_RIGHT_22_SEQUENCE=TGATGGAGTTCAGGTTGTGG
PRIMER_LEFT_22=177,19
PRIMER_RIGHT_22=637,20
PRIMER_LEFT_22_TM=59.147
PRIMER_RIGHT_22_TM=59.520
PRIMER_LEFT_22_GC_PERCENT=57.895
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=3.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=461
PRIMER_PAIR_22_PRODUCT_TM=84.0
PRIMER_PAIR_23_PENALTY=2.355333
PRIMER_LEFT_23_PENALTY=1.879714
PRIMER_RIGHT_23_PENALTY=0.475619
PRIMER_LEFT_23_SEQUENCE=CGTATAAATGACCTGCCTGG
PRIMER_RIGHT_23_SEQUENCE=ACTGGTACTGCGTCTCCAGC
PRIMER_LEFT_23=207,20
PRIMER_RIGHT_23=499,20
PRIMER_LEFT_23_TM=58.120
PRIMER_RIGHT_23_TM=60.476
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=2.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=293
PRIMER_PAIR_23_PRODUCT_TM=83.5
PRIMER_PAIR_24_PENALTY=2.363551
PRIMER_LEFT_24_PENALTY=1.853220
PRIMER_RIGHT_24_PENALTY=0.510331
PRIMER_LEFT_24_SEQUENCE=AGGAGATGGGATGGAGAGC
PRIMER_RIGHT_24_SEQUENCE=GGATGGTGAAGACCACGAAG
PRIMER_LEFT_24=175,19
PRIMER_RIGHT_24=883,20
PRIMER_LEFT_24_TM=59.147
PRIMER_RIGHT_24_TM=60.510
PRIMER_LEFT_24_GC_PERCENT=57.895
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=8.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=709
PRIMER_PAIR_24_PRODUCT_TM=85.1
PRIMER_PAIR_25_PENALTY=2.533215
PRIMER_LEFT_25_PENALTY=1.975728
PRIMER_RIGHT_25_PENALTY=0.557487
PRIMER_LEFT_25_SEQUENCE=GGATGGAAGTGCTTGGATG
PRIMER_RIGHT_25_SEQUENCE=CCGAGTGGTGGTTAGAGGTG
PRIMER_LEFT_25=129,19
PRIMER_RIGHT_25=367,20
PRIMER_LEFT_25_TM=59.024
PRIMER_RIGHT_25_TM=60.557
PRIMER_LEFT_25_GC_PERCENT=52.632
PRIMER_RIGHT_25_GC_PERCENT=60.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=2.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=239
PRIMER_PAIR_25_PRODUCT_TM=82.1
PRIMER_PAIR_26_PENALTY=2.595918
PRIMER_LEFT_26_PENALTY=2.006951
PRIMER_RIGHT_26_PENALTY=0.588967
PRIMER_LEFT_26_SEQUENCE=GTATAAATGACCTGCCTGGCTC
PRIMER_RIGHT_26_SEQUENCE=AGCTGGACCTTGAGCTTGTG
PRIMER_LEFT_26=208,22
PRIMER_RIGHT_26=965,20
PRIMER_LEFT_26_TM=59.993
PRIMER_RIGHT_26_TM=60.589
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=3.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=758
PRIMER_PAIR_26_PRODUCT_TM=85.2
PRIMER_PAIR_27_PENALTY=2.646531
PRIMER_LEFT_27_PENALTY=2.057564
PRIMER_RIGHT_27_PENALTY=0.588967
PRIMER_LEFT_27_SEQUENCE=AGCCTGAGTTGGCATTCG
PRIMER_RIGHT_27_SEQUENCE=CAGCTGGACCTTGAGCTTGT
PRIMER_LEFT_27=191,18
PRIMER_RIGHT_27=966,20
PRIMER_LEFT_27_TM=59.942
PRIMER_RIGHT_27_TM=60.589
PRIMER_LEFT_27_GC_PERCENT=55.556
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=2.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.6000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=776
PRIMER_PAIR_27_PRODUCT_TM=85.2
PRIMER_PAIR_28_PENALTY=2.707656
PRIMER_LEFT_28_PENALTY=2.106022
PRIMER_RIGHT_28_PENALTY=0.601633
PRIMER_LEFT_28_SEQUENCE=AGATGGGATGGAGAGCCTGA
PRIMER_RIGHT_28_SEQUENCE=AGGCACGTTAAGCTCAGCAC
PRIMER_LEFT_28=178,20
PRIMER_RIGHT_28=258,20
PRIMER_LEFT_28_TM=62.106
PRIMER_RIGHT_28_TM=60.602
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=81
PRIMER_PAIR_28_PRODUCT_TM=74.3
PRIMER_PAIR_29_PENALTY=2.707656
PRIMER_LEFT_29_PENALTY=2.106022
PRIMER_RIGHT_29_PENALTY=0.601633
PRIMER_LEFT_29_SEQUENCE=GAGATGGGATGGAGAGCCTG
PRIMER_RIGHT_29_SEQUENCE=GGCACGTTAAGCTCAGCACT
PRIMER_LEFT_29=177,20
PRIMER_RIGHT_29=257,20
PRIMER_LEFT_29_TM=62.106
PRIMER_RIGHT_29_TM=60.602
PRIMER_LEFT_29_GC_PERCENT=60.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=2.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=81
PRIMER_PAIR_29_PRODUCT_TM=74.8
PRIMER_PAIR_30_PENALTY=2.781272
PRIMER_LEFT_30_PENALTY=2.071143
PRIMER_RIGHT_30_PENALTY=0.710129
PRIMER_LEFT_30_SEQUENCE=GGAGAGCCTGAGTTGGCA
PRIMER_RIGHT_30_SEQUENCE=ACTTGATCCAGCAGAGCAGC
PRIMER_LEFT_30=187,18
PRIMER_RIGHT_30=751,20
PRIMER_LEFT_30_TM=60.071
PRIMER_RIGHT_30_TM=60.710
PRIMER_LEFT_30_GC_PERCENT=61.111
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=3.00
PRIMER_RIGHT_30_SELF_END=3.00
PRIMER_LEFT_30_END_STABILITY=10.0000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=565
PRIMER_PAIR_30_PRODUCT_TM=84.5
PRIMER_PAIR_31_PENALTY=2.793543
PRIMER_LEFT_31_PENALTY=2.071143
PRIMER_RIGHT_31_PENALTY=0.722400
PRIMER_LEFT_31_SEQUENCE=TGGAGAGCCTGAGTTGGC
PRIMER_RIGHT_31_SEQUENCE=CAGGTTGTGGATGTTGCTCA
PRIMER_LEFT_31=186,18
PRIMER_RIGHT_31=627,20
PRIMER_LEFT_31_TM=60.071
PRIMER_RIGHT_31_TM=60.722
PRIMER_LEFT_31_GC_PERCENT=61.111
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=2.00
PRIMER_RIGHT_31_SELF_END=2.00
PRIMER_LEFT_31_END_STABILITY=10.0000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=442
PRIMER_PAIR_31_PRODUCT_TM=84.2
PRIMER_PAIR_32_PENALTY=2.851602
PRIMER_LEFT_32_PENALTY=2.106022
PRIMER_RIGHT_32_PENALTY=0.745579
PRIMER_LEFT_32_SEQUENCE=GATGGGATGGAGAGCCTGAG
PRIMER_RIGHT_32_SEQUENCE=AGGAGCAGAGGGGTCGATAG
PRIMER_LEFT_32=179,20
PRIMER_RIGHT_32=276,20
PRIMER_LEFT_32_TM=62.106
PRIMER_RIGHT_32_TM=60.746
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=3.00
PRIMER_RIGHT_32_SELF_END=2.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=5.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=98
PRIMER_PAIR_32_PRODUCT_TM=76.4
PRIMER_PAIR_33_PENALTY=2.902052
PRIMER_LEFT_33_PENALTY=2.125665
PRIMER_RIGHT_33_PENALTY=0.776387
PRIMER_LEFT_33_SEQUENCE=GGATGGAGAGCCTGAGTTGG
PRIMER_RIGHT_33_SEQUENCE=ACCTCGGCCAGGAAGAGTAG
PRIMER_LEFT_33=183,20
PRIMER_RIGHT_33=728,20
PRIMER_LEFT_33_TM=62.126
PRIMER_RIGHT_33_TM=60.776
PRIMER_LEFT_33_GC_PERCENT=60.000
PRIMER_RIGHT_33_GC_PERCENT=60.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=5.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=546
PRIMER_PAIR_33_PRODUCT_TM=84.5
PRIMER_PAIR_34_PENALTY=2.926857
PRIMER_LEFT_34_PENALTY=2.125665
PRIMER_RIGHT_34_PENALTY=0.801192
PRIMER_LEFT_34_SEQUENCE=GGGATGGAGAGCCTGAGTTG
PRIMER_RIGHT_34_SEQUENCE=TCGCTGATGGAGTTCAGGTT
PRIMER_LEFT_34=182,20
PRIMER_RIGHT_34=641,20
PRIMER_LEFT_34_TM=62.126
PRIMER_RIGHT_34_TM=60.801
PRIMER_LEFT_34_GC_PERCENT=60.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=460
PRIMER_PAIR_34_PRODUCT_TM=84.2
PRIMER_PAIR_35_PENALTY=2.938687
PRIMER_LEFT_35_PENALTY=2.125665
PRIMER_RIGHT_35_PENALTY=0.813021
PRIMER_LEFT_35_SEQUENCE=TGGGATGGAGAGCCTGAGTT
PRIMER_RIGHT_35_SEQUENCE=CCCTGCTCAGGTAGAGCTTC
PRIMER_LEFT_35=181,20
PRIMER_RIGHT_35=406,20
PRIMER_LEFT_35_TM=62.126
PRIMER_RIGHT_35_TM=59.187
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=60.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=2.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=226
PRIMER_PAIR_35_PRODUCT_TM=82.1
PRIMER_PAIR_36_PENALTY=2.975455
PRIMER_LEFT_36_PENALTY=2.141376
PRIMER_RIGHT_36_PENALTY=0.834079
PRIMER_LEFT_36_SEQUENCE=TGGGATGGAGAGCCTGAG
PRIMER_RIGHT_36_SEQUENCE=GAGCGGTAGAAGTGGATGGT
PRIMER_LEFT_36=181,18
PRIMER_RIGHT_36=896,20
PRIMER_LEFT_36_TM=59.859
PRIMER_RIGHT_36_TM=59.166
PRIMER_LEFT_36_GC_PERCENT=61.111
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=3.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.8000
PRIMER_PAIR_36_COMPL_ANY=2.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=716
PRIMER_PAIR_36_PRODUCT_TM=85.1
PRIMER_PAIR_37_PENALTY=3.002161
PRIMER_LEFT_37_PENALTY=2.205825
PRIMER_RIGHT_37_PENALTY=0.796337
PRIMER_LEFT_37_SEQUENCE=GAAGTGCTTGGATGCGGT
PRIMER_RIGHT_37_SEQUENCE=TACGAATGCCAACTCAGGCT
PRIMER_LEFT_37=134,18
PRIMER_RIGHT_37=210,20
PRIMER_LEFT_37_TM=59.794
PRIMER_RIGHT_37_TM=60.796
PRIMER_LEFT_37_GC_PERCENT=55.556
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=3.00
PRIMER_LEFT_37_END_STABILITY=11.1000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=77
PRIMER_PAIR_37_PRODUCT_TM=76.1
PRIMER_PAIR_38_PENALTY=3.007846
PRIMER_LEFT_38_PENALTY=2.146683
PRIMER_RIGHT_38_PENALTY=0.861164
PRIMER_LEFT_38_SEQUENCE=TGGCATTCGTATAAATGACCTG
PRIMER_RIGHT_38_SEQUENCE=TTCAGGTTGTGGATGTTGCT
PRIMER_LEFT_38=200,22
PRIMER_RIGHT_38=629,20
PRIMER_LEFT_38_TM=59.853
PRIMER_RIGHT_38_TM=59.139
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=2.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=430
PRIMER_PAIR_38_PRODUCT_TM=84.2
PRIMER_PAIR_39_PENALTY=3.037638
PRIMER_LEFT_39_PENALTY=2.153955
PRIMER_RIGHT_39_PENALTY=0.883683
PRIMER_LEFT_39_SEQUENCE=GATGGGATGGAGAGCCTGA
PRIMER_RIGHT_39_SEQUENCE=GAACTTGATCCAGCAGAGCA
PRIMER_LEFT_39=179,19
PRIMER_RIGHT_39=753,20
PRIMER_LEFT_39_TM=61.154
PRIMER_RIGHT_39_TM=59.116
PRIMER_LEFT_39_GC_PERCENT=57.895
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=2.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=575
PRIMER_PAIR_39_PRODUCT_TM=84.4
PRIMER_PAIR_40_PENALTY=3.081219
PRIMER_LEFT_40_PENALTY=2.188530
PRIMER_RIGHT_40_PENALTY=0.892689
PRIMER_LEFT_40_SEQUENCE=TAAATGACCTGCCTGGCTC
PRIMER_RIGHT_40_SEQUENCE=CCGGTAATCCATGCCCTTAT
PRIMER_LEFT_40=211,19
PRIMER_RIGHT_40=315,20
PRIMER_LEFT_40_TM=58.811
PRIMER_RIGHT_40_TM=60.893
PRIMER_LEFT_40_GC_PERCENT=52.632
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=3.00
PRIMER_RIGHT_40_SELF_END=2.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=5.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=105
PRIMER_PAIR_40_PRODUCT_TM=78.8
PRIMER_PAIR_41_PENALTY=3.193213
PRIMER_LEFT_41_PENALTY=2.205825
PRIMER_RIGHT_41_PENALTY=0.987388
PRIMER_LEFT_41_SEQUENCE=GCCTGAGTTGGCATTCGT
PRIMER_RIGHT_41_SEQUENCE=AGTGGATGGTGAAGACCACG
PRIMER_LEFT_41=192,18
PRIMER_RIGHT_41=886,20
PRIMER_LEFT_41_TM=59.794
PRIMER_RIGHT_41_TM=60.987
PRIMER_LEFT_41_GC_PERCENT=55.556
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=2.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=695
PRIMER_PAIR_41_PRODUCT_TM=85.2
PRIMER_PAIR_42_PENALTY=3.231013
PRIMER_LEFT_42_PENALTY=2.217968
PRIMER_RIGHT_42_PENALTY=1.013045
PRIMER_LEFT_42_SEQUENCE=GCTCCCACCATGAGTGCT
PRIMER_RIGHT_42_SEQUENCE=GTTCAGGTTGTGGATGTTGC
PRIMER_LEFT_42=226,18
PRIMER_RIGHT_42=630,20
PRIMER_LEFT_42_TM=59.782
PRIMER_RIGHT_42_TM=58.987
PRIMER_LEFT_42_GC_PERCENT=61.111
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=2.00
PRIMER_RIGHT_42_SELF_END=2.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=405
PRIMER_PAIR_42_PRODUCT_TM=84.5
PRIMER_PAIR_43_PENALTY=3.268525
PRIMER_LEFT_43_PENALTY=2.250013
PRIMER_RIGHT_43_PENALTY=1.018512
PRIMER_LEFT_43_SEQUENCE=GGATGTGCGCAAGGAGAT
PRIMER_RIGHT_43_SEQUENCE=ATGGTGAAGACCACGAAGATG
PRIMER_LEFT_43=164,18
PRIMER_RIGHT_43=881,21
PRIMER_LEFT_43_TM=59.750
PRIMER_RIGHT_43_TM=59.981
PRIMER_LEFT_43_GC_PERCENT=55.556
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=2.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.3000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=718
PRIMER_PAIR_43_PRODUCT_TM=85.1
PRIMER_PAIR_44_PENALTY=3.287655
PRIMER_LEFT_44_PENALTY=2.441275
PRIMER_RIGHT_44_PENALTY=0.846380
PRIMER_LEFT_44_SEQUENCE=GGGATGGAAGTGCTTGGA
PRIMER_RIGHT_44_SEQUENCE=GAGCCAGGCAGGTCATTTAT
PRIMER_LEFT_44=128,18
PRIMER_RIGHT_44=229,20
PRIMER_LEFT_44_TM=59.559
PRIMER_RIGHT_44_TM=59.154
PRIMER_LEFT_44_GC_PERCENT=55.556
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=2.00
PRIMER_RIGHT_44_SELF_END=2.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=102
PRIMER_PAIR_44_PRODUCT_TM=77.7
PRIMER_PAIR_45_PENALTY=3.305926
PRIMER_LEFT_45_PENALTY=2.278334
PRIMER_RIGHT_45_PENALTY=1.027592
PRIMER_LEFT_45_SEQUENCE=GATGGAGAGCCTGAGTTGGC
PRIMER_RIGHT_45_SEQUENCE=GTTCAGGTTGTGGATGTTGCT
PRIMER_LEFT_45=184,20
PRIMER_RIGHT_45=630,21
PRIMER_LEFT_45_TM=62.278
PRIMER_RIGHT_45_TM=60.028
PRIMER_LEFT_45_GC_PERCENT=60.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=2.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=10.0000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=447
PRIMER_PAIR_45_PRODUCT_TM=84.2
PRIMER_PAIR_46_PENALTY=3.456958
PRIMER_LEFT_46_PENALTY=2.429366
PRIMER_RIGHT_46_PENALTY=1.027592
PRIMER_LEFT_46_SEQUENCE=TAAATGACCTGCCTGGCTCC
PRIMER_RIGHT_46_SEQUENCE=AGTTCAGGTTGTGGATGTTGC
PRIMER_LEFT_46=211,20
PRIMER_RIGHT_46=631,21
PRIMER_LEFT_46_TM=62.429
PRIMER_RIGHT_46_TM=60.028
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=2.00
PRIMER_RIGHT_46_SELF_END=2.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=421
PRIMER_PAIR_46_PRODUCT_TM=84.3
PRIMER_PAIR_47_PENALTY=3.528337
PRIMER_LEFT_47_PENALTY=2.481969
PRIMER_RIGHT_47_PENALTY=1.046368
PRIMER_LEFT_47_SEQUENCE=GCAAGGAGATGGGATGGAGA
PRIMER_RIGHT_47_SEQUENCE=CGATAGGCACGTTAAGCTCAG
PRIMER_LEFT_47=172,20
PRIMER_RIGHT_47=262,21
PRIMER_LEFT_47_TM=62.482
PRIMER_RIGHT_47_TM=60.046
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=3.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=91
PRIMER_PAIR_47_PRODUCT_TM=75.4
PRIMER_PAIR_48_PENALTY=3.533715
PRIMER_LEFT_48_PENALTY=2.484888
PRIMER_RIGHT_48_PENALTY=1.048827
PRIMER_LEFT_48_SEQUENCE=CAAGGAGATGGGATGGAGA
PRIMER_RIGHT_48_SEQUENCE=CTCGGCCAGGAAGAGTAGG
PRIMER_LEFT_48=173,19
PRIMER_RIGHT_48=726,19
PRIMER_LEFT_48_TM=58.515
PRIMER_RIGHT_48_TM=59.951
PRIMER_LEFT_48_GC_PERCENT=52.632
PRIMER_RIGHT_48_GC_PERCENT=63.158
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=554
PRIMER_PAIR_48_PRODUCT_TM=84.4
PRIMER_PAIR_49_PENALTY=3.537252
PRIMER_LEFT_49_PENALTY=2.488425
PRIMER_RIGHT_49_PENALTY=1.048827
PRIMER_LEFT_49_SEQUENCE=GCAAGGAGATGGGATGGA
PRIMER_RIGHT_49_SEQUENCE=CCTCGGCCAGGAAGAGTAG
PRIMER_LEFT_49=172,18
PRIMER_RIGHT_49=727,19
PRIMER_LEFT_49_TM=59.512
PRIMER_RIGHT_49_TM=59.951
PRIMER_LEFT_49_GC_PERCENT=55.556
PRIMER_RIGHT_49_GC_PERCENT=63.158
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=5.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=556
PRIMER_PAIR_49_PRODUCT_TM=84.5
PRIMER_PAIR_50_PENALTY=3.666838
PRIMER_LEFT_50_PENALTY=2.613581
PRIMER_RIGHT_50_PENALTY=1.053256
PRIMER_LEFT_50_SEQUENCE=CAGGGATGGAAGTGCTTGG
PRIMER_RIGHT_50_SEQUENCE=ATAGGCACGTTAAGCTCAGCA
PRIMER_LEFT_50=126,19
PRIMER_RIGHT_50=260,21
PRIMER_LEFT_50_TM=61.614
PRIMER_RIGHT_50_TM=60.053
PRIMER_LEFT_50_GC_PERCENT=57.895
PRIMER_RIGHT_50_GC_PERCENT=47.619
PRIMER_LEFT_50_SELF_ANY=4.00
PRIMER_RIGHT_50_SELF_ANY=5.00
PRIMER_LEFT_50_SELF_END=0.00
PRIMER_RIGHT_50_SELF_END=3.00
PRIMER_LEFT_50_END_STABILITY=8.5000
PRIMER_RIGHT_50_END_STABILITY=8.5000
PRIMER_PAIR_50_COMPL_ANY=4.00
PRIMER_PAIR_50_COMPL_END=3.00
PRIMER_PAIR_50_PRODUCT_SIZE=135
PRIMER_PAIR_50_PRODUCT_TM=78.5
PRIMER_PAIR_51_PENALTY=3.668226
PRIMER_LEFT_51_PENALTY=2.613581
PRIMER_RIGHT_51_PENALTY=1.054645
PRIMER_LEFT_51_SEQUENCE=CCAGGGATGGAAGTGCTTG
PRIMER_RIGHT_51_SEQUENCE=GTGGATGGTGAAGACCACG
PRIMER_LEFT_51=125,19
PRIMER_RIGHT_51=885,19
PRIMER_LEFT_51_TM=61.614
PRIMER_RIGHT_51_TM=59.945
PRIMER_LEFT_51_GC_PERCENT=57.895
PRIMER_RIGHT_51_GC_PERCENT=57.895
PRIMER_LEFT_51_SELF_ANY=4.00
PRIMER_RIGHT_51_SELF_ANY=4.00
PRIMER_LEFT_51_SELF_END=2.00
PRIMER_RIGHT_51_SELF_END=2.00
PRIMER_LEFT_51_END_STABILITY=8.5000
PRIMER_RIGHT_51_END_STABILITY=9.9000
PRIMER_PAIR_51_COMPL_ANY=3.00
PRIMER_PAIR_51_COMPL_END=2.00
PRIMER_PAIR_51_PRODUCT_SIZE=761
PRIMER_PAIR_51_PRODUCT_TM=85.2
PRIMER_PAIR_52_PENALTY=3.722775
PRIMER_LEFT_52_PENALTY=2.665121
PRIMER_RIGHT_52_PENALTY=1.057654
PRIMER_LEFT_52_SEQUENCE=TATAAATGACCTGCCTGGCT
PRIMER_RIGHT_52_SEQUENCE=GCCCTGCTCAGGTAGAGCTT
PRIMER_LEFT_52=209,20
PRIMER_RIGHT_52=407,20
PRIMER_LEFT_52_TM=57.335
PRIMER_RIGHT_52_TM=61.058
PRIMER_LEFT_52_GC_PERCENT=45.000
PRIMER_RIGHT_52_GC_PERCENT=60.000
PRIMER_LEFT_52_SELF_ANY=5.00
PRIMER_RIGHT_52_SELF_ANY=6.00
PRIMER_LEFT_52_SELF_END=3.00
PRIMER_RIGHT_52_SELF_END=2.00
PRIMER_LEFT_52_END_STABILITY=9.7000
PRIMER_RIGHT_52_END_STABILITY=8.2000
PRIMER_PAIR_52_COMPL_ANY=5.00
PRIMER_PAIR_52_COMPL_END=3.00
PRIMER_PAIR_52_PRODUCT_SIZE=199
PRIMER_PAIR_52_PRODUCT_TM=82.0
PRIMER_PAIR_53_PENALTY=3.746319
PRIMER_LEFT_53_PENALTY=2.679749
PRIMER_RIGHT_53_PENALTY=1.066570
PRIMER_LEFT_53_SEQUENCE=GAGCCTGAGTTGGCATTCGT
PRIMER_RIGHT_53_SEQUENCE=GCGGTAGAAGTGGATGGTGA
PRIMER_LEFT_53=190,20
PRIMER_RIGHT_53=894,20
PRIMER_LEFT_53_TM=62.680
PRIMER_RIGHT_53_TM=61.067
PRIMER_LEFT_53_GC_PERCENT=55.000
PRIMER_RIGHT_53_GC_PERCENT=55.000
PRIMER_LEFT_53_SELF_ANY=3.00
PRIMER_RIGHT_53_SELF_ANY=2.00
PRIMER_LEFT_53_SELF_END=0.00
PRIMER_RIGHT_53_SELF_END=1.00
PRIMER_LEFT_53_END_STABILITY=8.4000
PRIMER_RIGHT_53_END_STABILITY=7.9000
PRIMER_PAIR_53_COMPL_ANY=3.00
PRIMER_PAIR_53_COMPL_END=1.00
PRIMER_PAIR_53_PRODUCT_SIZE=705
PRIMER_PAIR_53_PRODUCT_TM=85.2
PRIMER_PAIR_54_PENALTY=3.797786
PRIMER_LEFT_54_PENALTY=2.694261
PRIMER_RIGHT_54_PENALTY=1.103525
PRIMER_LEFT_54_SEQUENCE=GATGGAGAGCCTGAGTTGG
PRIMER_RIGHT_54_SEQUENCE=CTCAGGTAGAGCTTCCGCC
PRIMER_LEFT_54=184,19
PRIMER_RIGHT_54=401,19
PRIMER_LEFT_54_TM=58.306
PRIMER_RIGHT_54_TM=60.104
PRIMER_LEFT_54_GC_PERCENT=57.895
PRIMER_RIGHT_54_GC_PERCENT=63.158
PRIMER_LEFT_54_SELF_ANY=3.00
PRIMER_RIGHT_54_SELF_ANY=4.00
PRIMER_LEFT_54_SELF_END=0.00
PRIMER_RIGHT_54_SELF_END=2.00
PRIMER_LEFT_54_END_STABILITY=8.2000
PRIMER_RIGHT_54_END_STABILITY=12.9000
PRIMER_PAIR_54_COMPL_ANY=6.00
PRIMER_PAIR_54_COMPL_END=2.00
PRIMER_PAIR_54_PRODUCT_SIZE=218
PRIMER_PAIR_54_PRODUCT_TM=81.8
PRIMER_PAIR_55_PENALTY=3.805118
PRIMER_LEFT_55_PENALTY=2.694261
PRIMER_RIGHT_55_PENALTY=1.110857
PRIMER_LEFT_55_SEQUENCE=GGATGGAGAGCCTGAGTTG
PRIMER_RIGHT_55_SEQUENCE=GGAAGAGTAGGATGCCAAGC
PRIMER_LEFT_55=183,19
PRIMER_RIGHT_55=718,20
PRIMER_LEFT_55_TM=58.306
PRIMER_RIGHT_55_TM=58.889
PRIMER_LEFT_55_GC_PERCENT=57.895
PRIMER_RIGHT_55_GC_PERCENT=55.000
PRIMER_LEFT_55_SELF_ANY=3.00
PRIMER_RIGHT_55_SELF_ANY=3.00
PRIMER_LEFT_55_SELF_END=0.00
PRIMER_RIGHT_55_SELF_END=2.00
PRIMER_LEFT_55_END_STABILITY=6.7000
PRIMER_RIGHT_55_END_STABILITY=8.5000
PRIMER_PAIR_55_COMPL_ANY=3.00
PRIMER_PAIR_55_COMPL_END=2.00
PRIMER_PAIR_55_PRODUCT_SIZE=536
PRIMER_PAIR_55_PRODUCT_TM=84.4
PRIMER_PAIR_56_PENALTY=3.814970
PRIMER_LEFT_56_PENALTY=2.700295
PRIMER_RIGHT_56_PENALTY=1.114674
PRIMER_LEFT_56_SEQUENCE=GCATTCGTATAAATGACCTGCC
PRIMER_RIGHT_56_SEQUENCE=TCGGCCAGGAAGAGTAGGAT
PRIMER_LEFT_56=202,22
PRIMER_RIGHT_56=725,20
PRIMER_LEFT_56_TM=60.700
PRIMER_RIGHT_56_TM=61.115
PRIMER_LEFT_56_GC_PERCENT=45.455
PRIMER_RIGHT_56_GC_PERCENT=55.000
PRIMER_LEFT_56_SELF_ANY=6.00
PRIMER_RIGHT_56_SELF_ANY=4.00
PRIMER_LEFT_56_SELF_END=2.00
PRIMER_RIGHT_56_SELF_END=2.00
PRIMER_LEFT_56_END_STABILITY=9.7000
PRIMER_RIGHT_56_END_STABILITY=7.8000
PRIMER_PAIR_56_COMPL_ANY=4.00
PRIMER_PAIR_56_COMPL_END=1.00
PRIMER_PAIR_56_PRODUCT_SIZE=524
PRIMER_PAIR_56_PRODUCT_TM=84.5
PRIMER_PAIR_57_PENALTY=3.836405
PRIMER_LEFT_57_PENALTY=2.726528
PRIMER_RIGHT_57_PENALTY=1.109878
PRIMER_LEFT_57_SEQUENCE=ACGTCCCAGGGATGGAAGT
PRIMER_RIGHT_57_SEQUENCE=TATACGAATGCCAACTCAGGC
PRIMER_LEFT_57=120,19
PRIMER_RIGHT_57=212,21
PRIMER_LEFT_57_TM=61.727
PRIMER_RIGHT_57_TM=60.110
PRIMER_LEFT_57_GC_PERCENT=57.895
PRIMER_RIGHT_57_GC_PERCENT=47.619
PRIMER_LEFT_57_SELF_ANY=7.00
PRIMER_RIGHT_57_SELF_ANY=4.00
PRIMER_LEFT_57_SELF_END=2.00
PRIMER_RIGHT_57_SELF_END=2.00
PRIMER_LEFT_57_END_STABILITY=6.4000
PRIMER_RIGHT_57_END_STABILITY=9.7000
PRIMER_PAIR_57_COMPL_ANY=4.00
PRIMER_PAIR_57_COMPL_END=3.00
PRIMER_PAIR_57_PRODUCT_SIZE=93
PRIMER_PAIR_57_PRODUCT_TM=77.3
PRIMER_PAIR_58_PENALTY=3.874414
PRIMER_LEFT_58_PENALTY=2.748413
PRIMER_RIGHT_58_PENALTY=1.126001
PRIMER_LEFT_58_SEQUENCE=GGGATGGAAGTGCTTGGATG
PRIMER_RIGHT_58_SEQUENCE=GCCAGGAAGAGTAGGATGCC
PRIMER_LEFT_58=128,20
PRIMER_RIGHT_58=722,20
PRIMER_LEFT_58_TM=62.748
PRIMER_RIGHT_58_TM=61.126
PRIMER_LEFT_58_GC_PERCENT=55.000
PRIMER_RIGHT_58_GC_PERCENT=60.000
PRIMER_LEFT_58_SELF_ANY=4.00
PRIMER_RIGHT_58_SELF_ANY=3.00
PRIMER_LEFT_58_SELF_END=2.00
PRIMER_RIGHT_58_SELF_END=2.00
PRIMER_LEFT_58_END_STABILITY=8.1000
PRIMER_RIGHT_58_END_STABILITY=9.6000
PRIMER_PAIR_58_COMPL_ANY=3.00
PRIMER_PAIR_58_COMPL_END=1.00
PRIMER_PAIR_58_PRODUCT_SIZE=595
PRIMER_PAIR_58_PRODUCT_TM=84.5
PRIMER_PAIR_59_PENALTY=3.900013
PRIMER_LEFT_59_PENALTY=2.774013
PRIMER_RIGHT_59_PENALTY=1.126001
PRIMER_LEFT_59_SEQUENCE=GTATAAATGACCTGCCTGGCT
PRIMER_RIGHT_59_SEQUENCE=GGCCAGGAAGAGTAGGATGC
PRIMER_LEFT_59=208,21
PRIMER_RIGHT_59=723,20
PRIMER_LEFT_59_TM=58.226
PRIMER_RIGHT_59_TM=61.126
PRIMER_LEFT_59_GC_PERCENT=47.619
PRIMER_RIGHT_59_GC_PERCENT=60.000
PRIMER_LEFT_59_SELF_ANY=5.00
PRIMER_RIGHT_59_SELF_ANY=4.00
PRIMER_LEFT_59_SELF_END=3.00
PRIMER_RIGHT_59_SELF_END=2.00
PRIMER_LEFT_59_END_STABILITY=9.7000
PRIMER_RIGHT_59_END_STABILITY=8.1000
PRIMER_PAIR_59_COMPL_ANY=7.00
PRIMER_PAIR_59_COMPL_END=3.00
PRIMER_PAIR_59_PRODUCT_SIZE=516
PRIMER_PAIR_59_PRODUCT_TM=84.6
PRIMER_PAIR_60_PENALTY=3.941862
PRIMER_LEFT_60_PENALTY=2.792938
PRIMER_RIGHT_60_PENALTY=1.148923
PRIMER_LEFT_60_SEQUENCE=AGAGCCTGAGTTGGCATTCG
PRIMER_RIGHT_60_SEQUENCE=GAACTTGATCCAGCAGAGCAG
PRIMER_LEFT_60=189,20
PRIMER_RIGHT_60=753,21
PRIMER_LEFT_60_TM=62.793
PRIMER_RIGHT_60_TM=60.149
PRIMER_LEFT_60_GC_PERCENT=55.000
PRIMER_RIGHT_60_GC_PERCENT=52.381
PRIMER_LEFT_60_SELF_ANY=3.00
PRIMER_RIGHT_60_SELF_ANY=6.00
PRIMER_LEFT_60_SELF_END=2.00
PRIMER_RIGHT_60_SELF_END=1.00
PRIMER_LEFT_60_END_STABILITY=8.6000
PRIMER_RIGHT_60_END_STABILITY=8.2000
PRIMER_PAIR_60_COMPL_ANY=4.00
PRIMER_PAIR_60_COMPL_END=0.00
PRIMER_PAIR_60_PRODUCT_SIZE=565
PRIMER_PAIR_60_PRODUCT_TM=84.4
PRIMER_PAIR_61_PENALTY=3.943966
PRIMER_LEFT_61_PENALTY=2.806404
PRIMER_RIGHT_61_PENALTY=1.137562
PRIMER_LEFT_61_SEQUENCE=ATGTGCGCAAGGAGATGG
PRIMER_RIGHT_61_SEQUENCE=AGGAAGAGTAGGATGCCAAGC
PRIMER_LEFT_61=166,18
PRIMER_RIGHT_61=719,21
PRIMER_LEFT_61_TM=60.806
PRIMER_RIGHT_61_TM=59.862
PRIMER_LEFT_61_GC_PERCENT=55.556
PRIMER_RIGHT_61_GC_PERCENT=52.381
PRIMER_LEFT_61_SELF_ANY=6.00
PRIMER_RIGHT_61_SELF_ANY=3.00
PRIMER_LEFT_61_SELF_END=0.00
PRIMER_RIGHT_61_SELF_END=2.00
PRIMER_LEFT_61_END_STABILITY=8.1000
PRIMER_RIGHT_61_END_STABILITY=8.5000
PRIMER_PAIR_61_COMPL_ANY=3.00
PRIMER_PAIR_61_COMPL_END=1.00
PRIMER_PAIR_61_PRODUCT_SIZE=554
PRIMER_PAIR_61_PRODUCT_TM=84.3
PRIMER_PAIR_62_PENALTY=3.992475
PRIMER_LEFT_62_PENALTY=2.827930
PRIMER_RIGHT_62_PENALTY=1.164545
PRIMER_LEFT_62_SEQUENCE=GGAGATGGGATGGAGAGCCT
PRIMER_RIGHT_62_SEQUENCE=ATGGAGTTCAGGTTGTGGATG
PRIMER_LEFT_62=176,20
PRIMER_RIGHT_62=635,21
PRIMER_LEFT_62_TM=62.828
PRIMER_RIGHT_62_TM=59.835
PRIMER_LEFT_62_GC_PERCENT=60.000
PRIMER_RIGHT_62_GC_PERCENT=47.619
PRIMER_LEFT_62_SELF_ANY=3.00
PRIMER_RIGHT_62_SELF_ANY=3.00
PRIMER_LEFT_62_SELF_END=3.00
PRIMER_RIGHT_62_SELF_END=0.00
PRIMER_LEFT_62_END_STABILITY=9.4000
PRIMER_RIGHT_62_END_STABILITY=8.1000
PRIMER_PAIR_62_COMPL_ANY=3.00
PRIMER_PAIR_62_COMPL_END=2.00
PRIMER_PAIR_62_PRODUCT_SIZE=460
PRIMER_PAIR_62_PRODUCT_TM=84.1
PRIMER_PAIR_63_PENALTY=3.997588
PRIMER_LEFT_63_PENALTY=2.827930
PRIMER_RIGHT_63_PENALTY=1.169658
PRIMER_LEFT_63_SEQUENCE=AGGAGATGGGATGGAGAGCC
PRIMER_RIGHT_63_SEQUENCE=GCACGTTAAGCTCAGCACTC
PRIMER_LEFT_63=175,20
PRIMER_RIGHT_63=256,20
PRIMER_LEFT_63_TM=62.828
PRIMER_RIGHT_63_TM=58.830
PRIMER_LEFT_63_GC_PERCENT=60.000
PRIMER_RIGHT_63_GC_PERCENT=55.000
PRIMER_LEFT_63_SELF_ANY=2.00
PRIMER_RIGHT_63_SELF_ANY=5.00
PRIMER_LEFT_63_SELF_END=1.00
PRIMER_RIGHT_63_SELF_END=0.00
PRIMER_LEFT_63_END_STABILITY=9.4000
PRIMER_RIGHT_63_END_STABILITY=6.4000
PRIMER_PAIR_63_COMPL_ANY=4.00
PRIMER_PAIR_63_COMPL_END=3.00
PRIMER_PAIR_63_PRODUCT_SIZE=82
PRIMER_PAIR_63_PRODUCT_TM=74.6
PRIMER_PAIR_64_PENALTY=4.011724
PRIMER_LEFT_64_PENALTY=2.840217
PRIMER_RIGHT_64_PENALTY=1.171507
PRIMER_LEFT_64_SEQUENCE=AAGTGCTTGGATGCGGTG
PRIMER_RIGHT_64_SEQUENCE=CTTGATCCAGCAGAGCAGC
PRIMER_LEFT_64=135,18
PRIMER_RIGHT_64=750,19
PRIMER_LEFT_64_TM=60.840
PRIMER_RIGHT_64_TM=59.828
PRIMER_LEFT_64_GC_PERCENT=55.556
PRIMER_RIGHT_64_GC_PERCENT=57.895
PRIMER_LEFT_64_SELF_ANY=4.00
PRIMER_RIGHT_64_SELF_ANY=6.00
PRIMER_LEFT_64_SELF_END=0.00
PRIMER_RIGHT_64_SELF_END=3.00
PRIMER_LEFT_64_END_STABILITY=9.9000
PRIMER_RIGHT_64_END_STABILITY=9.7000
PRIMER_PAIR_64_COMPL_ANY=6.00
PRIMER_PAIR_64_COMPL_END=3.00
PRIMER_PAIR_64_PRODUCT_SIZE=616
PRIMER_PAIR_64_PRODUCT_TM=84.5
PRIMER_PAIR_65_PENALTY=4.042943
PRIMER_LEFT_65_PENALTY=2.857001
PRIMER_RIGHT_65_PENALTY=1.185942
PRIMER_LEFT_65_SEQUENCE=CCTGAGTTGGCATTCGTATAAA
PRIMER_RIGHT_65_SEQUENCE=GGTACTGGTACTGCGTCTCCA
PRIMER_LEFT_65=193,22
PRIMER_RIGHT_65=502,21
PRIMER_LEFT_65_TM=59.143
PRIMER_RIGHT_65_TM=60.186
PRIMER_LEFT_65_GC_PERCENT=40.909
PRIMER_RIGHT_65_GC_PERCENT=57.143
PRIMER_LEFT_65_SELF_ANY=4.00
PRIMER_RIGHT_65_SELF_ANY=6.00
PRIMER_LEFT_65_SELF_END=2.00
PRIMER_RIGHT_65_SELF_END=2.00
PRIMER_LEFT_65_END_STABILITY=6.2000
PRIMER_RIGHT_65_END_STABILITY=8.2000
PRIMER_PAIR_65_COMPL_ANY=3.00
PRIMER_PAIR_65_COMPL_END=0.00
PRIMER_PAIR_65_PRODUCT_SIZE=310
PRIMER_PAIR_65_PRODUCT_TM=83.4
PRIMER_PAIR_66_PENALTY=4.075959
PRIMER_LEFT_66_PENALTY=2.890161
PRIMER_RIGHT_66_PENALTY=1.185797
PRIMER_LEFT_66_SEQUENCE=CGTCCCAGGGATGGAAGT
PRIMER_RIGHT_66_SEQUENCE=TACGAATGCCAACTCAGGC
PRIMER_LEFT_66=121,18
PRIMER_RIGHT_66=210,19
PRIMER_LEFT_66_TM=60.890
PRIMER_RIGHT_66_TM=59.814
PRIMER_LEFT_66_GC_PERCENT=61.111
PRIMER_RIGHT_66_GC_PERCENT=52.632
PRIMER_LEFT_66_SELF_ANY=7.00
PRIMER_RIGHT_66_SELF_ANY=3.00
PRIMER_LEFT_66_SELF_END=1.00
PRIMER_RIGHT_66_SELF_END=2.00
PRIMER_LEFT_66_END_STABILITY=6.4000
PRIMER_RIGHT_66_END_STABILITY=9.7000
PRIMER_PAIR_66_COMPL_ANY=4.00
PRIMER_PAIR_66_COMPL_END=3.00
PRIMER_PAIR_66_PRODUCT_SIZE=90
PRIMER_PAIR_66_PRODUCT_TM=77.8
PRIMER_PAIR_67_PENALTY=4.078421
PRIMER_LEFT_67_PENALTY=2.890161
PRIMER_RIGHT_67_PENALTY=1.188260
PRIMER_LEFT_67_SEQUENCE=ACGTCCCAGGGATGGAAG
PRIMER_RIGHT_67_SEQUENCE=ACCTCGGCCAGGAAGAGTA
PRIMER_LEFT_67=120,18
PRIMER_RIGHT_67=728,19
PRIMER_LEFT_67_TM=60.890
PRIMER_RIGHT_67_TM=59.812
PRIMER_LEFT_67_GC_PERCENT=61.111
PRIMER_RIGHT_67_GC_PERCENT=57.895
PRIMER_LEFT_67_SELF_ANY=7.00
PRIMER_RIGHT_67_SELF_ANY=6.00
PRIMER_LEFT_67_SELF_END=0.00
PRIMER_RIGHT_67_SELF_END=2.00
PRIMER_LEFT_67_END_STABILITY=8.2000
PRIMER_RIGHT_67_END_STABILITY=5.4000
PRIMER_PAIR_67_COMPL_ANY=4.00
PRIMER_PAIR_67_COMPL_END=0.00
PRIMER_PAIR_67_PRODUCT_SIZE=609
PRIMER_PAIR_67_PRODUCT_TM=84.6
PRIMER_PAIR_68_PENALTY=4.100063
PRIMER_LEFT_68_PENALTY=2.911803
PRIMER_RIGHT_68_PENALTY=1.188260
PRIMER_LEFT_68_SEQUENCE=GAGCCTGAGTTGGCATTCG
PRIMER_RIGHT_68_SEQUENCE=AGGGAGCGGTAGAAGTGGA
PRIMER_LEFT_68=190,19
PRIMER_RIGHT_68=899,19
PRIMER_LEFT_68_TM=61.912
PRIMER_RIGHT_68_TM=59.812
PRIMER_LEFT_68_GC_PERCENT=57.895
PRIMER_RIGHT_68_GC_PERCENT=57.895
PRIMER_LEFT_68_SELF_ANY=3.00
PRIMER_RIGHT_68_SELF_ANY=2.00
PRIMER_LEFT_68_SELF_END=2.00
PRIMER_RIGHT_68_SELF_END=0.00
PRIMER_LEFT_68_END_STABILITY=8.6000
PRIMER_RIGHT_68_END_STABILITY=7.9000
PRIMER_PAIR_68_COMPL_ANY=3.00
PRIMER_PAIR_68_COMPL_END=1.00
PRIMER_PAIR_68_PRODUCT_SIZE=710
PRIMER_PAIR_68_PRODUCT_TM=85.2
PRIMER_PAIR_69_PENALTY=4.123274
PRIMER_LEFT_69_PENALTY=2.922864
PRIMER_RIGHT_69_PENALTY=1.200410
PRIMER_LEFT_69_SEQUENCE=ATGGGATGGAGAGCCTGA
PRIMER_RIGHT_69_SEQUENCE=TGAAGACCACGAAGATGAGG
PRIMER_LEFT_69=180,18
PRIMER_RIGHT_69=877,20
PRIMER_LEFT_69_TM=59.077
PRIMER_RIGHT_69_TM=58.800
PRIMER_LEFT_69_GC_PERCENT=55.556
PRIMER_RIGHT_69_GC_PERCENT=50.000
PRIMER_LEFT_69_SELF_ANY=3.00
PRIMER_RIGHT_69_SELF_ANY=2.00
PRIMER_LEFT_69_SELF_END=1.00
PRIMER_RIGHT_69_SELF_END=0.00
PRIMER_LEFT_69_END_STABILITY=8.2000
PRIMER_RIGHT_69_END_STABILITY=8.2000
PRIMER_PAIR_69_COMPL_ANY=3.00
PRIMER_PAIR_69_COMPL_END=3.00
PRIMER_PAIR_69_PRODUCT_SIZE=698
PRIMER_PAIR_69_PRODUCT_TM=85.1
PRIMER_PAIR_70_PENALTY=4.138152
PRIMER_LEFT_70_PENALTY=2.922864
PRIMER_RIGHT_70_PENALTY=1.215288
PRIMER_LEFT_70_SEQUENCE=GATGGGATGGAGAGCCTG
PRIMER_RIGHT_70_SEQUENCE=GGGACTCGCTGATGGAGTT
PRIMER_LEFT_70=179,18
PRIMER_RIGHT_70=646,19
PRIMER_LEFT_70_TM=59.077
PRIMER_RIGHT_70_TM=60.215
PRIMER_LEFT_70_GC_PERCENT=61.111
PRIMER_RIGHT_70_GC_PERCENT=57.895
PRIMER_LEFT_70_SELF_ANY=3.00
PRIMER_RIGHT_70_SELF_ANY=5.00
PRIMER_LEFT_70_SELF_END=1.00
PRIMER_RIGHT_70_SELF_END=3.00
PRIMER_LEFT_70_END_STABILITY=9.7000
PRIMER_RIGHT_70_END_STABILITY=6.4000
PRIMER_PAIR_70_COMPL_ANY=3.00
PRIMER_PAIR_70_COMPL_END=0.00
PRIMER_PAIR_70_PRODUCT_SIZE=468
PRIMER_PAIR_70_PRODUCT_TM=84.2
PRIMER_PAIR_71_PENALTY=4.173923
PRIMER_LEFT_71_PENALTY=2.944273
PRIMER_RIGHT_71_PENALTY=1.229650
PRIMER_LEFT_71_SEQUENCE=GGAGATGGGATGGAGAGCC
PRIMER_RIGHT_71_SEQUENCE=GAGCAGAGGGGTCGATAGG
PRIMER_LEFT_71=176,19
PRIMER_RIGHT_71=274,19
PRIMER_LEFT_71_TM=61.944
PRIMER_RIGHT_71_TM=59.770
PRIMER_LEFT_71_GC_PERCENT=63.158
PRIMER_RIGHT_71_GC_PERCENT=63.158
PRIMER_LEFT_71_SELF_ANY=2.00
PRIMER_RIGHT_71_SELF_ANY=4.00
PRIMER_LEFT_71_SELF_END=1.00
PRIMER_RIGHT_71_SELF_END=0.00
PRIMER_LEFT_71_END_STABILITY=9.4000
PRIMER_RIGHT_71_END_STABILITY=7.1000
PRIMER_PAIR_71_COMPL_ANY=3.00
PRIMER_PAIR_71_COMPL_END=2.00
PRIMER_PAIR_71_PRODUCT_SIZE=99
PRIMER_PAIR_71_PRODUCT_TM=76.6
PRIMER_PAIR_72_PENALTY=4.195505
PRIMER_LEFT_72_PENALTY=2.965855
PRIMER_RIGHT_72_PENALTY=1.229650
PRIMER_LEFT_72_SEQUENCE=AACGTCCCAGGGATGGAAGT
PRIMER_RIGHT_72_SEQUENCE=GGAGCAGAGGGGTCGATAG
PRIMER_LEFT_72=119,20
PRIMER_RIGHT_72=275,19
PRIMER_LEFT_72_TM=62.966
PRIMER_RIGHT_72_TM=59.770
PRIMER_LEFT_72_GC_PERCENT=55.000
PRIMER_RIGHT_72_GC_PERCENT=63.158
PRIMER_LEFT_72_SELF_ANY=7.00
PRIMER_RIGHT_72_SELF_ANY=4.00
PRIMER_LEFT_72_SELF_END=2.00
PRIMER_RIGHT_72_SELF_END=2.00
PRIMER_LEFT_72_END_STABILITY=6.4000
PRIMER_RIGHT_72_END_STABILITY=5.6000
PRIMER_PAIR_72_COMPL_ANY=4.00
PRIMER_PAIR_72_COMPL_END=0.00
PRIMER_PAIR_72_PRODUCT_SIZE=157
PRIMER_PAIR_72_PRODUCT_TM=79.8
PRIMER_PAIR_73_PENALTY=4.269459
PRIMER_LEFT_73_PENALTY=3.039809
PRIMER_RIGHT_73_PENALTY=1.229650
PRIMER_LEFT_73_SEQUENCE=CCCAGGGATGGAAGTGCT
PRIMER_RIGHT_73_SEQUENCE=AGGAGCAGAGGGGTCGATA
PRIMER_LEFT_73=124,18
PRIMER_RIGHT_73=276,19
PRIMER_LEFT_73_TM=61.040
PRIMER_RIGHT_73_TM=59.770
PRIMER_LEFT_73_GC_PERCENT=61.111
PRIMER_RIGHT_73_GC_PERCENT=57.895
PRIMER_LEFT_73_SELF_ANY=5.00
PRIMER_RIGHT_73_SELF_ANY=4.00
PRIMER_LEFT_73_SELF_END=2.00
PRIMER_RIGHT_73_SELF_END=2.00
PRIMER_LEFT_73_END_STABILITY=7.9000
PRIMER_RIGHT_73_END_STABILITY=7.6000
PRIMER_PAIR_73_COMPL_ANY=4.00
PRIMER_PAIR_73_COMPL_END=1.00
PRIMER_PAIR_73_PRODUCT_SIZE=153
PRIMER_PAIR_73_PRODUCT_TM=79.8
PRIMER_PAIR_74_PENALTY=4.301436
PRIMER_LEFT_74_PENALTY=3.045630
PRIMER_RIGHT_74_PENALTY=1.255806
PRIMER_LEFT_74_SEQUENCE=AGAGCCTGAGTTGGCATTC
PRIMER_RIGHT_74_SEQUENCE=GCTCAGGTAGAGCTTCCGC
PRIMER_LEFT_74=189,19
PRIMER_RIGHT_74=402,19
PRIMER_LEFT_74_TM=57.954
PRIMER_RIGHT_74_TM=60.256
PRIMER_LEFT_74_GC_PERCENT=52.632
PRIMER_RIGHT_74_GC_PERCENT=63.158
PRIMER_LEFT_74_SELF_ANY=3.00
PRIMER_RIGHT_74_SELF_ANY=4.00
PRIMER_LEFT_74_SELF_END=2.00
PRIMER_RIGHT_74_SELF_END=2.00
PRIMER_LEFT_74_END_STABILITY=6.9000
PRIMER_RIGHT_74_END_STABILITY=11.4000
PRIMER_PAIR_74_COMPL_ANY=6.00
PRIMER_PAIR_74_COMPL_END=0.00
PRIMER_PAIR_74_PRODUCT_SIZE=214
PRIMER_PAIR_74_PRODUCT_TM=81.8
PRIMER_PAIR_75_PENALTY=4.323206
PRIMER_LEFT_75_PENALTY=3.045630
PRIMER_RIGHT_75_PENALTY=1.277576
PRIMER_LEFT_75_SEQUENCE=GAGAGCCTGAGTTGGCATT
PRIMER_RIGHT_75_SEQUENCE=CGAGTGGTGGTTAGAGGTGA
PRIMER_LEFT_75=188,19
PRIMER_RIGHT_75=366,20
PRIMER_LEFT_75_TM=57.954
PRIMER_RIGHT_75_TM=58.722
PRIMER_LEFT_75_GC_PERCENT=52.632
PRIMER_RIGHT_75_GC_PERCENT=55.000
PRIMER_LEFT_75_SELF_ANY=3.00
PRIMER_RIGHT_75_SELF_ANY=2.00
PRIMER_LEFT_75_SELF_END=1.00
PRIMER_RIGHT_75_SELF_END=1.00
PRIMER_LEFT_75_END_STABILITY=8.4000
PRIMER_RIGHT_75_END_STABILITY=7.9000
PRIMER_PAIR_75_COMPL_ANY=3.00
PRIMER_PAIR_75_COMPL_END=1.00
PRIMER_PAIR_75_PRODUCT_SIZE=179
PRIMER_PAIR_75_PRODUCT_TM=81.1
PRIMER_PAIR_76_PENALTY=4.404428
PRIMER_LEFT_76_PENALTY=3.159514
PRIMER_RIGHT_76_PENALTY=1.244914
PRIMER_LEFT_76_SEQUENCE=GATGTGCGCAAGGAGATG
PRIMER_RIGHT_76_SEQUENCE=AGGCACGTTAAGCTCAGCA
PRIMER_LEFT_76=165,18
PRIMER_RIGHT_76=258,19
PRIMER_LEFT_76_TM=58.840
PRIMER_RIGHT_76_TM=59.755
PRIMER_LEFT_76_GC_PERCENT=55.556
PRIMER_RIGHT_76_GC_PERCENT=52.632
PRIMER_LEFT_76_SELF_ANY=6.00
PRIMER_RIGHT_76_SELF_ANY=5.00
PRIMER_LEFT_76_SELF_END=0.00
PRIMER_RIGHT_76_SELF_END=3.00
PRIMER_LEFT_76_END_STABILITY=6.6000
PRIMER_RIGHT_76_END_STABILITY=8.5000
PRIMER_PAIR_76_COMPL_ANY=4.00
PRIMER_PAIR_76_COMPL_END=2.00
PRIMER_PAIR_76_PRODUCT_SIZE=94
PRIMER_PAIR_76_PRODUCT_TM=75.8
PRIMER_PAIR_77_PENALTY=4.460864
PRIMER_LEFT_77_PENALTY=3.173109
PRIMER_RIGHT_77_PENALTY=1.287755
PRIMER_LEFT_77_SEQUENCE=ATGGAAGTGCTTGGATGCG
PRIMER_RIGHT_77_SEQUENCE=GTGAAGACCACGAAGATGAGG
PRIMER_LEFT_77=131,19
PRIMER_RIGHT_77=878,21
PRIMER_LEFT_77_TM=62.173
PRIMER_RIGHT_77_TM=59.712
PRIMER_LEFT_77_GC_PERCENT=52.632
PRIMER_RIGHT_77_GC_PERCENT=52.381
PRIMER_LEFT_77_SELF_ANY=4.00
PRIMER_RIGHT_77_SELF_ANY=3.00
PRIMER_LEFT_77_SELF_END=2.00
PRIMER_RIGHT_77_SELF_END=0.00
PRIMER_LEFT_77_END_STABILITY=10.1000
PRIMER_RIGHT_77_END_STABILITY=8.2000
PRIMER_PAIR_77_COMPL_ANY=3.00
PRIMER_PAIR_77_COMPL_END=0.00
PRIMER_PAIR_77_PRODUCT_SIZE=748
PRIMER_PAIR_77_PRODUCT_TM=85.1
PRIMER_PAIR_78_PENALTY=4.486063
PRIMER_LEFT_78_PENALTY=3.198308
PRIMER_RIGHT_78_PENALTY=1.287755
PRIMER_LEFT_78_SEQUENCE=TTCGTATAAATGACCTGCCTGG
PRIMER_RIGHT_78_SEQUENCE=GGTGAAGACCACGAAGATGAG
PRIMER_LEFT_78=205,22
PRIMER_RIGHT_78=879,21
PRIMER_LEFT_78_TM=61.198
PRIMER_RIGHT_78_TM=59.712
PRIMER_LEFT_78_GC_PERCENT=45.455
PRIMER_RIGHT_78_GC_PERCENT=52.381
PRIMER_LEFT_78_SELF_ANY=4.00
PRIMER_RIGHT_78_SELF_ANY=3.00
PRIMER_LEFT_78_SELF_END=3.00
PRIMER_RIGHT_78_SELF_END=0.00
PRIMER_LEFT_78_END_STABILITY=9.7000
PRIMER_RIGHT_78_END_STABILITY=6.6000
PRIMER_PAIR_78_COMPL_ANY=5.00
PRIMER_PAIR_78_COMPL_END=3.00
PRIMER_PAIR_78_PRODUCT_SIZE=675
PRIMER_PAIR_78_PRODUCT_TM=85.3
PRIMER_PAIR_79_PENALTY=4.490383
PRIMER_LEFT_79_PENALTY=3.200839
PRIMER_RIGHT_79_PENALTY=1.289544
PRIMER_LEFT_79_SEQUENCE=AATGACCTGCCTGGCTCC
PRIMER_RIGHT_79_SEQUENCE=CAGCTGGACCTTGAGCTTG
PRIMER_LEFT_79=213,18
PRIMER_RIGHT_79=966,19
PRIMER_LEFT_79_TM=61.201
PRIMER_RIGHT_79_TM=59.710
PRIMER_LEFT_79_GC_PERCENT=61.111
PRIMER_RIGHT_79_GC_PERCENT=57.895
PRIMER_LEFT_79_SELF_ANY=5.00
PRIMER_RIGHT_79_SELF_ANY=6.00
PRIMER_LEFT_79_SELF_END=2.00
PRIMER_RIGHT_79_SELF_END=2.00
PRIMER_LEFT_79_END_STABILITY=9.4000
PRIMER_RIGHT_79_END_STABILITY=8.5000
PRIMER_PAIR_79_COMPL_ANY=4.00
PRIMER_PAIR_79_COMPL_END=2.00
PRIMER_PAIR_79_PRODUCT_SIZE=754
PRIMER_PAIR_79_PRODUCT_TM=85.4
PRIMER_PAIR_80_PENALTY=4.556874
PRIMER_LEFT_80_PENALTY=3.199824
PRIMER_RIGHT_80_PENALTY=1.357049
PRIMER_LEFT_80_SEQUENCE=GTCCCAGGGATGGAAGTG
PRIMER_RIGHT_80_SEQUENCE=GGTGAAGACCACGAAGATGA
PRIMER_LEFT_80=122,18
PRIMER_RIGHT_80=879,20
PRIMER_LEFT_80_TM=58.800
PRIMER_RIGHT_80_TM=58.643
PRIMER_LEFT_80_GC_PERCENT=61.111
PRIMER_RIGHT_80_GC_PERCENT=50.000
PRIMER_LEFT_80_SELF_ANY=7.00
PRIMER_RIGHT_80_SELF_ANY=3.00
PRIMER_LEFT_80_SELF_END=0.00
PRIMER_RIGHT_80_SELF_END=1.00
PRIMER_LEFT_80_END_STABILITY=6.7000
PRIMER_RIGHT_80_END_STABILITY=6.6000
PRIMER_PAIR_80_COMPL_ANY=3.00
PRIMER_PAIR_80_COMPL_END=0.00
PRIMER_PAIR_80_PRODUCT_SIZE=758
PRIMER_PAIR_80_PRODUCT_TM=85.2
PRIMER_PAIR_81_PENALTY=4.561659
PRIMER_LEFT_81_PENALTY=3.204610
PRIMER_RIGHT_81_PENALTY=1.357049
PRIMER_LEFT_81_SEQUENCE=TTCGTATAAATGACCTGCCTG
PRIMER_RIGHT_81_SEQUENCE=GATGGTGAAGACCACGAAGA
PRIMER_LEFT_81=205,21
PRIMER_RIGHT_81=882,20
PRIMER_LEFT_81_TM=57.795
PRIMER_RIGHT_81_TM=58.643
PRIMER_LEFT_81_GC_PERCENT=42.857
PRIMER_RIGHT_81_GC_PERCENT=50.000
PRIMER_LEFT_81_SELF_ANY=4.00
PRIMER_RIGHT_81_SELF_ANY=4.00
PRIMER_LEFT_81_SELF_END=1.00
PRIMER_RIGHT_81_SELF_END=0.00
PRIMER_LEFT_81_END_STABILITY=9.7000
PRIMER_RIGHT_81_END_STABILITY=6.7000
PRIMER_PAIR_81_COMPL_ANY=5.00
PRIMER_PAIR_81_COMPL_END=2.00
PRIMER_PAIR_81_PRODUCT_SIZE=678
PRIMER_PAIR_81_PRODUCT_TM=85.2
PRIMER_PAIR_82_PENALTY=4.573274
PRIMER_LEFT_82_PENALTY=3.204610
PRIMER_RIGHT_82_PENALTY=1.368664
PRIMER_LEFT_82_SEQUENCE=CCTGAGTTGGCATTCGTATAA
PRIMER_RIGHT_82_SEQUENCE=CGAGTGGTGGTTAGAGGTGAC
PRIMER_LEFT_82=193,21
PRIMER_RIGHT_82=366,21
PRIMER_LEFT_82_TM=57.795
PRIMER_RIGHT_82_TM=59.631
PRIMER_LEFT_82_GC_PERCENT=42.857
PRIMER_RIGHT_82_GC_PERCENT=57.143
PRIMER_LEFT_82_SELF_ANY=4.00
PRIMER_RIGHT_82_SELF_ANY=3.00
PRIMER_LEFT_82_SELF_END=2.00
PRIMER_RIGHT_82_SELF_END=3.00
PRIMER_LEFT_82_END_STABILITY=5.2000
PRIMER_RIGHT_82_END_STABILITY=6.1000
PRIMER_PAIR_82_COMPL_ANY=4.00
PRIMER_PAIR_82_COMPL_END=1.00
PRIMER_PAIR_82_PRODUCT_SIZE=174
PRIMER_PAIR_82_PRODUCT_TM=81.0
PRIMER_PAIR_83_PENALTY=4.592877
PRIMER_LEFT_83_PENALTY=3.227824
PRIMER_RIGHT_83_PENALTY=1.365052
PRIMER_LEFT_83_SEQUENCE=AACGTCCCAGGGATGGAAG
PRIMER_RIGHT_83_SEQUENCE=ATGCCAACTCAGGCTCTCC
PRIMER_LEFT_83=119,19
PRIMER_RIGHT_83=205,19
PRIMER_LEFT_83_TM=62.228
PRIMER_RIGHT_83_TM=60.365
PRIMER_LEFT_83_GC_PERCENT=57.895
PRIMER_RIGHT_83_GC_PERCENT=57.895
PRIMER_LEFT_83_SELF_ANY=7.00
PRIMER_RIGHT_83_SELF_ANY=3.00
PRIMER_LEFT_83_SELF_END=0.00
PRIMER_RIGHT_83_SELF_END=0.00
PRIMER_LEFT_83_END_STABILITY=8.2000
PRIMER_RIGHT_83_END_STABILITY=7.9000
PRIMER_PAIR_83_COMPL_ANY=4.00
PRIMER_PAIR_83_COMPL_END=3.00
PRIMER_PAIR_83_PRODUCT_SIZE=87
PRIMER_PAIR_83_PRODUCT_TM=77.5
PRIMER_PAIR_84_PENALTY=4.627137
PRIMER_LEFT_84_PENALTY=3.251078
PRIMER_RIGHT_84_PENALTY=1.376058
PRIMER_LEFT_84_SEQUENCE=AAATGACCTGCCTGGCTC
PRIMER_RIGHT_84_SEQUENCE=GGACTCGCTGATGGAGTTCA
PRIMER_LEFT_84=212,18
PRIMER_RIGHT_84=645,20
PRIMER_LEFT_84_TM=58.749
PRIMER_RIGHT_84_TM=61.376
PRIMER_LEFT_84_GC_PERCENT=55.556
PRIMER_RIGHT_84_GC_PERCENT=55.000
PRIMER_LEFT_84_SELF_ANY=5.00
PRIMER_RIGHT_84_SELF_ANY=5.00
PRIMER_LEFT_84_SELF_END=3.00
PRIMER_RIGHT_84_SELF_END=3.00
PRIMER_LEFT_84_END_STABILITY=9.4000
PRIMER_RIGHT_84_END_STABILITY=6.7000
PRIMER_PAIR_84_COMPL_ANY=4.00
PRIMER_PAIR_84_COMPL_END=2.00
PRIMER_PAIR_84_PRODUCT_SIZE=434
PRIMER_PAIR_84_PRODUCT_TM=84.4
PRIMER_PAIR_85_PENALTY=4.665035
PRIMER_LEFT_85_PENALTY=3.284613
PRIMER_RIGHT_85_PENALTY=1.380422
PRIMER_LEFT_85_SEQUENCE=ATGGGATGGAGAGCCTGAGTT
PRIMER_RIGHT_85_SEQUENCE=GATGCAGGTGCTGATGAGG
PRIMER_LEFT_85=180,21
PRIMER_RIGHT_85=588,19
PRIMER_LEFT_85_TM=62.285
PRIMER_RIGHT_85_TM=60.380
PRIMER_LEFT_85_GC_PERCENT=52.381
PRIMER_RIGHT_85_GC_PERCENT=57.895
PRIMER_LEFT_85_SELF_ANY=3.00
PRIMER_RIGHT_85_SELF_ANY=4.00
PRIMER_LEFT_85_SELF_END=0.00
PRIMER_RIGHT_85_SELF_END=2.00
PRIMER_LEFT_85_END_STABILITY=6.4000
PRIMER_RIGHT_85_END_STABILITY=8.2000
PRIMER_PAIR_85_COMPL_ANY=4.00
PRIMER_PAIR_85_COMPL_END=1.00
PRIMER_PAIR_85_PRODUCT_SIZE=409
PRIMER_PAIR_85_PRODUCT_TM=84.2
PRIMER_PAIR_86_PENALTY=4.674115
PRIMER_LEFT_86_PENALTY=3.293693
PRIMER_RIGHT_86_PENALTY=1.380422
PRIMER_LEFT_86_SEQUENCE=AACGTCCCAGGGATGGAA
PRIMER_RIGHT_86_SEQUENCE=AGGATGCAGGTGCTGATGA
PRIMER_LEFT_86=119,18
PRIMER_RIGHT_86=590,19
PRIMER_LEFT_86_TM=61.294
PRIMER_RIGHT_86_TM=60.380
PRIMER_LEFT_86_GC_PERCENT=55.556
PRIMER_RIGHT_86_GC_PERCENT=52.632
PRIMER_LEFT_86_SELF_ANY=7.00
PRIMER_RIGHT_86_SELF_ANY=4.00
PRIMER_LEFT_86_SELF_END=2.00
PRIMER_RIGHT_86_SELF_END=2.00
PRIMER_LEFT_86_END_STABILITY=8.5000
PRIMER_RIGHT_86_END_STABILITY=6.6000
PRIMER_PAIR_86_COMPL_ANY=3.00
PRIMER_PAIR_86_COMPL_END=0.00
PRIMER_PAIR_86_PRODUCT_SIZE=472
PRIMER_PAIR_86_PRODUCT_TM=84.3
PRIMER_PAIR_87_PENALTY=4.717135
PRIMER_LEFT_87_PENALTY=3.331781
PRIMER_RIGHT_87_PENALTY=1.385354
PRIMER_LEFT_87_SEQUENCE=CCCACCATGAGTGCTGAG
PRIMER_RIGHT_87_SEQUENCE=TGGACCTTGAGCTTGTGGA
PRIMER_LEFT_87=229,18
PRIMER_RIGHT_87=962,19
PRIMER_LEFT_87_TM=58.668
PRIMER_RIGHT_87_TM=60.385
PRIMER_LEFT_87_GC_PERCENT=61.111
PRIMER_RIGHT_87_GC_PERCENT=52.632
PRIMER_LEFT_87_SELF_ANY=6.00
PRIMER_RIGHT_87_SELF_ANY=4.00
PRIMER_LEFT_87_SELF_END=3.00
PRIMER_RIGHT_87_SELF_END=0.00
PRIMER_LEFT_87_END_STABILITY=6.7000
PRIMER_RIGHT_87_END_STABILITY=7.9000
PRIMER_PAIR_87_COMPL_ANY=4.00
PRIMER_PAIR_87_COMPL_END=0.00
PRIMER_PAIR_87_PRODUCT_SIZE=734
PRIMER_PAIR_87_PRODUCT_TM=85.4
PRIMER_PAIR_88_PENALTY=4.721721
PRIMER_LEFT_88_PENALTY=3.331781
PRIMER_RIGHT_88_PENALTY=1.389940
PRIMER_LEFT_88_SEQUENCE=CTCCCACCATGAGTGCTG
PRIMER_RIGHT_88_SEQUENCE=TGGACCTTGAGCTTGTGGAG
PRIMER_LEFT_88=227,18
PRIMER_RIGHT_88=962,20
PRIMER_LEFT_88_TM=58.668
PRIMER_RIGHT_88_TM=61.390
PRIMER_LEFT_88_GC_PERCENT=61.111
PRIMER_RIGHT_88_GC_PERCENT=55.000
PRIMER_LEFT_88_SELF_ANY=6.00
PRIMER_RIGHT_88_SELF_ANY=4.00
PRIMER_LEFT_88_SELF_END=3.00
PRIMER_RIGHT_88_SELF_END=0.00
PRIMER_LEFT_88_END_STABILITY=8.5000
PRIMER_RIGHT_88_END_STABILITY=8.2000
PRIMER_PAIR_88_COMPL_ANY=4.00
PRIMER_PAIR_88_COMPL_END=1.00
PRIMER_PAIR_88_PRODUCT_SIZE=736
PRIMER_PAIR_88_PRODUCT_TM=85.4
PRIMER_PAIR_89_PENALTY=4.723310
PRIMER_LEFT_89_PENALTY=3.333370
PRIMER_RIGHT_89_PENALTY=1.389940
PRIMER_LEFT_89_SEQUENCE=GCCTGAGTTGGCATTCGTATAA
PRIMER_RIGHT_89_SEQUENCE=CTGGACCTTGAGCTTGTGGA
PRIMER_LEFT_89=192,22
PRIMER_RIGHT_89=963,20
PRIMER_LEFT_89_TM=61.333
PRIMER_RIGHT_89_TM=61.390
PRIMER_LEFT_89_GC_PERCENT=45.455
PRIMER_RIGHT_89_GC_PERCENT=55.000
PRIMER_LEFT_89_SELF_ANY=4.00
PRIMER_RIGHT_89_SELF_ANY=4.00
PRIMER_LEFT_89_SELF_END=2.00
PRIMER_RIGHT_89_SELF_END=0.00
PRIMER_LEFT_89_END_STABILITY=5.2000
PRIMER_RIGHT_89_END_STABILITY=7.9000
PRIMER_PAIR_89_COMPL_ANY=2.00
PRIMER_PAIR_89_COMPL_END=1.00
PRIMER_PAIR_89_PRODUCT_SIZE=772
PRIMER_PAIR_89_PRODUCT_TM=85.2
PRIMER_PAIR_90_PENALTY=4.810150
PRIMER_LEFT_90_PENALTY=3.413779
PRIMER_RIGHT_90_PENALTY=1.396371
PRIMER_LEFT_90_SEQUENCE=AGGGATGGAAGTGCTTGG
PRIMER_RIGHT_90_SEQUENCE=ATGCAGGTGCTGATGAGGAG
PRIMER_LEFT_90=127,18
PRIMER_RIGHT_90=587,20
PRIMER_LEFT_90_TM=58.586
PRIMER_RIGHT_90_TM=61.396
PRIMER_LEFT_90_GC_PERCENT=55.556
PRIMER_RIGHT_90_GC_PERCENT=55.000
PRIMER_LEFT_90_SELF_ANY=4.00
PRIMER_RIGHT_90_SELF_ANY=4.00
PRIMER_LEFT_90_SELF_END=0.00
PRIMER_RIGHT_90_SELF_END=2.00
PRIMER_LEFT_90_END_STABILITY=8.5000
PRIMER_RIGHT_90_END_STABILITY=7.9000
PRIMER_PAIR_90_COMPL_ANY=3.00
PRIMER_PAIR_90_COMPL_END=0.00
PRIMER_PAIR_90_PRODUCT_SIZE=461
PRIMER_PAIR_90_PRODUCT_TM=84.3
PRIMER_PAIR_91_PENALTY=4.913881
PRIMER_LEFT_91_PENALTY=3.514925
PRIMER_RIGHT_91_PENALTY=1.398956
PRIMER_LEFT_91_SEQUENCE=AAATGACCTGCCTGGCTCC
PRIMER_RIGHT_91_SEQUENCE=CGGTAGAAGTGGATGGTGAAG
PRIMER_LEFT_91=212,19
PRIMER_RIGHT_91=893,21
PRIMER_LEFT_91_TM=62.515
PRIMER_RIGHT_91_TM=59.601
PRIMER_LEFT_91_GC_PERCENT=57.895
PRIMER_RIGHT_91_GC_PERCENT=52.381
PRIMER_LEFT_91_SELF_ANY=5.00
PRIMER_RIGHT_91_SELF_ANY=2.00
PRIMER_LEFT_91_SELF_END=2.00
PRIMER_RIGHT_91_SELF_END=0.00
PRIMER_LEFT_91_END_STABILITY=9.4000
PRIMER_RIGHT_91_END_STABILITY=7.0000
PRIMER_PAIR_91_COMPL_ANY=3.00
PRIMER_PAIR_91_COMPL_END=2.00
PRIMER_PAIR_91_PRODUCT_SIZE=682
PRIMER_PAIR_91_PRODUCT_TM=85.3
PRIMER_PAIR_92_PENALTY=4.959245
PRIMER_LEFT_92_PENALTY=3.548727
PRIMER_RIGHT_92_PENALTY=1.410518
PRIMER_LEFT_92_SEQUENCE=CACCATGAGTGCTGAGCTT
PRIMER_RIGHT_92_SEQUENCE=AGAGGTGACCAGTTCCAGGT
PRIMER_LEFT_92=231,19
PRIMER_RIGHT_92=354,20
PRIMER_LEFT_92_TM=57.451
PRIMER_RIGHT_92_TM=58.589
PRIMER_LEFT_92_GC_PERCENT=52.632
PRIMER_RIGHT_92_GC_PERCENT=55.000
PRIMER_LEFT_92_SELF_ANY=6.00
PRIMER_RIGHT_92_SELF_ANY=5.00
PRIMER_LEFT_92_SELF_END=2.00
PRIMER_RIGHT_92_SELF_END=3.00
PRIMER_LEFT_92_END_STABILITY=8.2000
PRIMER_RIGHT_92_END_STABILITY=7.9000
PRIMER_PAIR_92_COMPL_ANY=4.00
PRIMER_PAIR_92_COMPL_END=2.00
PRIMER_PAIR_92_PRODUCT_SIZE=124
PRIMER_PAIR_92_PRODUCT_TM=80.5
PRIMER_PAIR_93_PENALTY=4.979019
PRIMER_LEFT_93_PENALTY=3.565929
PRIMER_RIGHT_93_PENALTY=1.413090
PRIMER_LEFT_93_SEQUENCE=ATAAATGACCTGCCTGGCTCC
PRIMER_RIGHT_93_SEQUENCE=CTGGTACTGCGTCTCCAGC
PRIMER_LEFT_93=210,21
PRIMER_RIGHT_93=498,19
PRIMER_LEFT_93_TM=62.566
PRIMER_RIGHT_93_TM=59.587
PRIMER_LEFT_93_GC_PERCENT=52.381
PRIMER_RIGHT_93_GC_PERCENT=63.158
PRIMER_LEFT_93_SELF_ANY=5.00
PRIMER_RIGHT_93_SELF_ANY=4.00
PRIMER_LEFT_93_SELF_END=2.00
PRIMER_RIGHT_93_SELF_END=2.00
PRIMER_LEFT_93_END_STABILITY=9.4000
PRIMER_RIGHT_93_END_STABILITY=9.7000
PRIMER_PAIR_93_COMPL_ANY=4.00
PRIMER_PAIR_93_COMPL_END=1.00
PRIMER_PAIR_93_PRODUCT_SIZE=289
PRIMER_PAIR_93_PRODUCT_TM=83.5
PRIMER_PAIR_94_PENALTY=5.004099
PRIMER_LEFT_94_PENALTY=3.588984
PRIMER_RIGHT_94_PENALTY=1.415114
PRIMER_LEFT_94_SEQUENCE=GAGCCTGAGTTGGCATTCGTA
PRIMER_RIGHT_94_SEQUENCE=TGGAGTTCAGGTTGTGGATGT
PRIMER_LEFT_94=190,21
PRIMER_RIGHT_94=634,21
PRIMER_LEFT_94_TM=62.589
PRIMER_RIGHT_94_TM=60.415
PRIMER_LEFT_94_GC_PERCENT=52.381
PRIMER_RIGHT_94_GC_PERCENT=47.619
PRIMER_LEFT_94_SELF_ANY=3.00
PRIMER_RIGHT_94_SELF_ANY=3.00
PRIMER_LEFT_94_SELF_END=2.00
PRIMER_RIGHT_94_SELF_END=0.00
PRIMER_LEFT_94_END_STABILITY=7.4000
PRIMER_RIGHT_94_END_STABILITY=6.3000
PRIMER_PAIR_94_COMPL_ANY=5.00
PRIMER_PAIR_94_COMPL_END=1.00
PRIMER_PAIR_94_PRODUCT_SIZE=445
PRIMER_PAIR_94_PRODUCT_TM=84.2
PRIMER_PAIR_95_PENALTY=5.156071
PRIMER_LEFT_95_PENALTY=3.724668
PRIMER_RIGHT_95_PENALTY=1.431404
PRIMER_LEFT_95_SEQUENCE=CATTCGTATAAATGACCTGCCTG
PRIMER_RIGHT_95_SEQUENCE=CGGTAGAAGTGGATGGTGAA
PRIMER_LEFT_95=203,23
PRIMER_RIGHT_95=893,20
PRIMER_LEFT_95_TM=60.725
PRIMER_RIGHT_95_TM=58.569
PRIMER_LEFT_95_GC_PERCENT=43.478
PRIMER_RIGHT_95_GC_PERCENT=50.000
PRIMER_LEFT_95_SELF_ANY=6.00
PRIMER_RIGHT_95_SELF_ANY=2.00
PRIMER_LEFT_95_SELF_END=1.00
PRIMER_RIGHT_95_SELF_END=0.00
PRIMER_LEFT_95_END_STABILITY=9.7000
PRIMER_RIGHT_95_END_STABILITY=6.7000
PRIMER_PAIR_95_COMPL_ANY=3.00
PRIMER_PAIR_95_COMPL_END=0.00
PRIMER_PAIR_95_PRODUCT_SIZE=691
PRIMER_PAIR_95_PRODUCT_TM=85.1
PRIMER_PAIR_96_PENALTY=5.174301
PRIMER_LEFT_96_PENALTY=3.785894
PRIMER_RIGHT_96_PENALTY=1.388407
PRIMER_LEFT_96_SEQUENCE=GATGTGCGCAAGGAGATGG
PRIMER_RIGHT_96_SEQUENCE=GGCACGTTAAGCTCAGCAC
PRIMER_LEFT_96=165,19
PRIMER_RIGHT_96=257,19
PRIMER_LEFT_96_TM=62.786
PRIMER_RIGHT_96_TM=59.612
PRIMER_LEFT_96_GC_PERCENT=57.895
PRIMER_RIGHT_96_GC_PERCENT=57.895
PRIMER_LEFT_96_SELF_ANY=6.00
PRIMER_RIGHT_96_SELF_ANY=5.00
PRIMER_LEFT_96_SELF_END=0.00
PRIMER_RIGHT_96_SELF_END=1.00
PRIMER_LEFT_96_END_STABILITY=8.1000
PRIMER_RIGHT_96_END_STABILITY=7.9000
PRIMER_PAIR_96_COMPL_ANY=4.00
PRIMER_PAIR_96_COMPL_END=1.00
PRIMER_PAIR_96_PRODUCT_SIZE=93
PRIMER_PAIR_96_PRODUCT_TM=75.9
PRIMER_PAIR_97_PENALTY=5.207404
PRIMER_LEFT_97_PENALTY=4.813185
PRIMER_RIGHT_97_PENALTY=0.394220
PRIMER_LEFT_97_SEQUENCE=GGGAAGGAACGTCCCAGG
PRIMER_RIGHT_97_SEQUENCE=CTCTCCATCCCATCTCCTTG
PRIMER_LEFT_97=112,18
PRIMER_RIGHT_97=192,20
PRIMER_LEFT_97_TM=62.813
PRIMER_RIGHT_97_TM=59.606
PRIMER_LEFT_97_GC_PERCENT=66.667
PRIMER_RIGHT_97_GC_PERCENT=55.000
PRIMER_LEFT_97_SELF_ANY=6.00
PRIMER_RIGHT_97_SELF_ANY=2.00
PRIMER_LEFT_97_SELF_END=3.00
PRIMER_RIGHT_97_SELF_END=0.00
PRIMER_LEFT_97_END_STABILITY=9.7000
PRIMER_RIGHT_97_END_STABILITY=8.5000
PRIMER_PAIR_97_COMPL_ANY=6.00
PRIMER_PAIR_97_COMPL_END=3.00
PRIMER_PAIR_97_PRODUCT_SIZE=81
PRIMER_PAIR_97_PRODUCT_TM=77.8
PRIMER_PAIR_98_PENALTY=5.214462
PRIMER_LEFT_98_PENALTY=3.782399
PRIMER_RIGHT_98_PENALTY=1.432063
PRIMER_LEFT_98_SEQUENCE=ATAAATGACCTGCCTGGCT
PRIMER_RIGHT_98_SEQUENCE=AGGTTGTGGATGTTGCTCAC
PRIMER_LEFT_98=210,19
PRIMER_RIGHT_98=626,20
PRIMER_LEFT_98_TM=57.218
PRIMER_RIGHT_98_TM=58.568
PRIMER_LEFT_98_GC_PERCENT=47.368
PRIMER_RIGHT_98_GC_PERCENT=50.000
PRIMER_LEFT_98_SELF_ANY=5.00
PRIMER_RIGHT_98_SELF_ANY=3.00
PRIMER_LEFT_98_SELF_END=3.00
PRIMER_RIGHT_98_SELF_END=0.00
PRIMER_LEFT_98_END_STABILITY=9.7000
PRIMER_RIGHT_98_END_STABILITY=6.4000
PRIMER_PAIR_98_COMPL_ANY=4.00
PRIMER_PAIR_98_COMPL_END=0.00
PRIMER_PAIR_98_PRODUCT_SIZE=417
PRIMER_PAIR_98_PRODUCT_TM=84.3
PRIMER_PAIR_99_PENALTY=5.221100
PRIMER_LEFT_99_PENALTY=3.785894
PRIMER_RIGHT_99_PENALTY=1.435206
PRIMER_LEFT_99_SEQUENCE=GGATGTGCGCAAGGAGATG
PRIMER_RIGHT_99_SEQUENCE=TGGTGGTTAGAGGTGACCAG
PRIMER_LEFT_99=164,19
PRIMER_RIGHT_99=362,20
PRIMER_LEFT_99_TM=62.786
PRIMER_RIGHT_99_TM=58.565
PRIMER_LEFT_99_GC_PERCENT=57.895
PRIMER_RIGHT_99_GC_PERCENT=55.000
PRIMER_LEFT_99_SELF_ANY=6.00
PRIMER_RIGHT_99_SELF_ANY=5.00
PRIMER_LEFT_99_SELF_END=0.00
PRIMER_RIGHT_99_SELF_END=2.00
PRIMER_LEFT_99_END_STABILITY=6.6000
PRIMER_RIGHT_99_END_STABILITY=7.9000
PRIMER_PAIR_99_COMPL_ANY=2.00
PRIMER_PAIR_99_COMPL_END=1.00
PRIMER_PAIR_99_PRODUCT_SIZE=199
PRIMER_PAIR_99_PRODUCT_TM=81.2
=
SEQUENCE_ID=ok_regions_test3
SEQUENCE_TEMPLATE=GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCAAAAAAAAAAAAAAAA
PRIMER_NUM_RETURN=900
PRIMER_PRODUCT_SIZE_RANGE=75-100 150-250 300-400 500-600 650-800
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=237,452,,;,,237,452;238,216,200,1500;0,1000,238,216
PRIMER_MIN_THREE_PRIME_DISTANCE=0
PRIMER_LEFT_EXPLAIN=considered 35740, GC content failed 759, low tm 702, high tm 4088, high any compl 30, high end compl 294, not in any ok left region 29065, ok 802
PRIMER_RIGHT_EXPLAIN=considered 35740, GC content failed 17, low tm 2090, high tm 7100, high any compl 36, high end compl 297, not in any ok right region 23605, ok 2595
PRIMER_PAIR_EXPLAIN=considered 1353277, unacceptable product size 1352154, high any compl 1, high end compl 105, primer in pair overlaps a primer in a better pair 6734138, ok 1017
PRIMER_LEFT_NUM_RETURNED=802
PRIMER_RIGHT_NUM_RETURNED=802
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=802
PRIMER_PAIR_0_PENALTY=0.033124
PRIMER_LEFT_0_PENALTY=0.024663
PRIMER_RIGHT_0_PENALTY=0.008461
PRIMER_LEFT_0_SEQUENCE=CTCGTTCCTGACAAGTGCAA
PRIMER_RIGHT_0_SEQUENCE=TCACTGCATGTCTCTTTGGC
PRIMER_LEFT_0=581,20
PRIMER_RIGHT_0=678,20
PRIMER_LEFT_0_TM=60.025
PRIMER_RIGHT_0_TM=59.992
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_0_PRODUCT_TM=73.9
PRIMER_PAIR_1_PENALTY=0.111900
PRIMER_LEFT_1_PENALTY=0.008461
PRIMER_RIGHT_1_PENALTY=0.103439
PRIMER_LEFT_1_SEQUENCE=GCCAAAGAGACATGCAGTGA
PRIMER_RIGHT_1_SEQUENCE=CTACCCCTCGGAACTTGTCA
PRIMER_LEFT_1=659,20
PRIMER_RIGHT_1=746,20
PRIMER_LEFT_1_TM=59.992
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_1_PRODUCT_TM=74.5
PRIMER_PAIR_2_PENALTY=0.197287
PRIMER_LEFT_2_PENALTY=0.172624
PRIMER_RIGHT_2_PENALTY=0.024663
PRIMER_LEFT_2_SEQUENCE=CATCCCCACTTTGTGATTCC
PRIMER_RIGHT_2_SEQUENCE=TTGCACTTGTCAGGAACGAG
PRIMER_LEFT_2=521,20
PRIMER_RIGHT_2=600,20
PRIMER_LEFT_2_TM=60.173
PRIMER_RIGHT_2_TM=60.025
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_2_PRODUCT_TM=72.4
PRIMER_PAIR_3_PENALTY=0.315491
PRIMER_LEFT_3_PENALTY=0.112124
PRIMER_RIGHT_3_PENALTY=0.203367
PRIMER_LEFT_3_SEQUENCE=CAGATTGCCATGTTCTGTGG
PRIMER_RIGHT_3_SEQUENCE=ATCAATGCAGGTTTTGGTCC
PRIMER_LEFT_3=296,20
PRIMER_RIGHT_3=391,20
PRIMER_LEFT_3_TM=60.112
PRIMER_RIGHT_3_TM=59.797
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_3_PRODUCT_TM=72.9
PRIMER_PAIR_4_PENALTY=0.335297
PRIMER_LEFT_4_PENALTY=0.172624
PRIMER_RIGHT_4_PENALTY=0.162673
PRIMER_LEFT_4_SEQUENCE=CCATCCCCACTTTGTGATTC
PRIMER_RIGHT_4_SEQUENCE=CTTGTCAGGAACGAGAAGGG
PRIMER_LEFT_4=520,20
PRIMER_RIGHT_4=595,20
PRIMER_LEFT_4_TM=60.173
PRIMER_RIGHT_4_TM=59.837
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
PRIMER_PAIR_4_PRODUCT_TM=72.5
PRIMER_PAIR_5_PENALTY=0.459489
PRIMER_LEFT_5_PENALTY=0.398877
PRIMER_RIGHT_5_PENALTY=0.060612
PRIMER_LEFT_5_SEQUENCE=TCCCTACCGCTGCTTAGTTG
PRIMER_RIGHT_5_SEQUENCE=GTTTCGCAAACATCCATCCT
PRIMER_LEFT_5=538,20
PRIMER_RIGHT_5=636,20
PRIMER_LEFT_5_TM=60.399
PRIMER_RIGHT_5_TM=59.939
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.8000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=99
PRIMER_PAIR_5_PRODUCT_TM=73.3
PRIMER_PAIR_6_PENALTY=0.463993
PRIMER_LEFT_6_PENALTY=0.220845
PRIMER_RIGHT_6_PENALTY=0.243148
PRIMER_LEFT_6_SEQUENCE=ATGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_6_SEQUENCE=GCAATACTGCAGGATGCCTT
PRIMER_LEFT_6=336,20
PRIMER_RIGHT_6=418,20
PRIMER_LEFT_6_TM=59.779
PRIMER_RIGHT_6_TM=60.243
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=83
PRIMER_PAIR_6_PRODUCT_TM=72.4
PRIMER_PAIR_7_PENALTY=0.513556
PRIMER_LEFT_7_PENALTY=0.452945
PRIMER_RIGHT_7_PENALTY=0.060612
PRIMER_LEFT_7_SEQUENCE=CCGCTGCTTAGTTGGTGAGT
PRIMER_RIGHT_7_SEQUENCE=AGTTTCGCAAACATCCATCC
PRIMER_LEFT_7=544,20
PRIMER_RIGHT_7=637,20
PRIMER_LEFT_7_TM=60.453
PRIMER_RIGHT_7_TM=59.939
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=94
PRIMER_PAIR_7_PRODUCT_TM=72.7
PRIMER_PAIR_8_PENALTY=0.523640
PRIMER_LEFT_8_PENALTY=0.237616
PRIMER_RIGHT_8_PENALTY=0.286024
PRIMER_LEFT_8_SEQUENCE=TGGAGGTACCCACTGATGGT
PRIMER_RIGHT_8_SEQUENCE=ATGTGCATGTTCAGTCTGCC
PRIMER_LEFT_8=252,20
PRIMER_RIGHT_8=333,20
PRIMER_LEFT_8_TM=60.238
PRIMER_RIGHT_8_TM=59.714
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.8000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=82
PRIMER_PAIR_8_PRODUCT_TM=74.6
PRIMER_PAIR_9_PENALTY=0.532592
PRIMER_LEFT_9_PENALTY=0.452945
PRIMER_RIGHT_9_PENALTY=0.079648
PRIMER_LEFT_9_SEQUENCE=ACCGCTGCTTAGTTGGTGAG
PRIMER_RIGHT_9_SEQUENCE=CATCCATCCTCTCCTGGTGT
PRIMER_LEFT_9=543,20
PRIMER_RIGHT_9=626,20
PRIMER_LEFT_9_TM=60.453
PRIMER_RIGHT_9_TM=59.920
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=84
PRIMER_PAIR_9_PRODUCT_TM=72.3
PRIMER_PAIR_10_PENALTY=0.580565
PRIMER_LEFT_10_PENALTY=0.407941
PRIMER_RIGHT_10_PENALTY=0.172624
PRIMER_LEFT_10_SEQUENCE=GGTAGAAGCCAACCAACCAG
PRIMER_RIGHT_10_SEQUENCE=GAATCACAAAGTGGGGATGG
PRIMER_LEFT_10=454,20
PRIMER_RIGHT_10=539,20
PRIMER_LEFT_10_TM=59.592
PRIMER_RIGHT_10_TM=60.173
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=86
PRIMER_PAIR_10_PRODUCT_TM=76.3
PRIMER_PAIR_11_PENALTY=0.591934
PRIMER_LEFT_11_PENALTY=0.512286
PRIMER_RIGHT_11_PENALTY=0.079648
PRIMER_LEFT_11_SEQUENCE=TACCGCTGCTTAGTTGGTGA
PRIMER_RIGHT_11_SEQUENCE=ACATCCATCCTCTCCTGGTG
PRIMER_LEFT_11=542,20
PRIMER_RIGHT_11=627,20
PRIMER_LEFT_11_TM=59.488
PRIMER_RIGHT_11_TM=59.920
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=86
PRIMER_PAIR_11_PRODUCT_TM=72.0
PRIMER_PAIR_12_PENALTY=0.606764
PRIMER_LEFT_12_PENALTY=0.434141
PRIMER_RIGHT_12_PENALTY=0.172624
PRIMER_LEFT_12_SEQUENCE=CACCAATGTGGTAGAAGCCA
PRIMER_RIGHT_12_SEQUENCE=GGAATCACAAAGTGGGGATG
PRIMER_LEFT_12=445,20
PRIMER_RIGHT_12=540,20
PRIMER_LEFT_12_TM=59.566
PRIMER_RIGHT_12_TM=60.173
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=96
PRIMER_PAIR_12_PRODUCT_TM=76.7
PRIMER_PAIR_13_PENALTY=0.641488
PRIMER_LEFT_13_PENALTY=0.420643
PRIMER_RIGHT_13_PENALTY=0.220845
PRIMER_LEFT_13_SEQUENCE=ACTGATGGTAATGCTGGCCT
PRIMER_RIGHT_13_SEQUENCE=CCACTTCCCATTCTGGACAT
PRIMER_LEFT_13=263,20
PRIMER_RIGHT_13=355,20
PRIMER_LEFT_13_TM=59.579
PRIMER_RIGHT_13_TM=59.779
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_END_STABILITY=10.9000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=93
PRIMER_PAIR_13_PRODUCT_TM=74.6
PRIMER_PAIR_14_PENALTY=0.642681
PRIMER_LEFT_14_PENALTY=0.215902
PRIMER_RIGHT_14_PENALTY=0.426779
PRIMER_LEFT_14_SEQUENCE=CCCCACTTTGTGATTCCCTA
PRIMER_RIGHT_14_SEQUENCE=TTTGCACTTGTCAGGAACGA
PRIMER_LEFT_14=524,20
PRIMER_RIGHT_14=601,20
PRIMER_LEFT_14_TM=59.784
PRIMER_RIGHT_14_TM=60.427
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=78
PRIMER_PAIR_14_PRODUCT_TM=72.2
PRIMER_PAIR_15_PENALTY=0.696384
PRIMER_LEFT_15_PENALTY=0.480482
PRIMER_RIGHT_15_PENALTY=0.215902
PRIMER_LEFT_15_SEQUENCE=CAACCAGTGACCATCCAGAA
PRIMER_RIGHT_15_SEQUENCE=TAGGGAATCACAAAGTGGGG
PRIMER_LEFT_15=467,20
PRIMER_RIGHT_15=543,20
PRIMER_LEFT_15_TM=59.520
PRIMER_RIGHT_15_TM=59.784
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=11.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=77
PRIMER_PAIR_15_PRODUCT_TM=75.5
PRIMER_PAIR_16_PENALTY=0.704589
PRIMER_LEFT_16_PENALTY=0.376749
PRIMER_RIGHT_16_PENALTY=0.327840
PRIMER_LEFT_16_SEQUENCE=CTGGAGGTACCCACTGATGG
PRIMER_RIGHT_16_SEQUENCE=GTGCATGTTCAGTCTGCCAC
PRIMER_LEFT_16=251,20
PRIMER_RIGHT_16=331,20
PRIMER_LEFT_16_TM=60.377
PRIMER_RIGHT_16_TM=60.328
PRIMER_LEFT_16_GC_PERCENT=60.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=81
PRIMER_PAIR_16_PRODUCT_TM=75.3
PRIMER_PAIR_17_PENALTY=0.749777
PRIMER_LEFT_17_PENALTY=0.203367
PRIMER_RIGHT_17_PENALTY=0.546410
PRIMER_LEFT_17_SEQUENCE=GGACCAAAACCTGCATTGAT
PRIMER_RIGHT_17_SEQUENCE=GGTTGGTTGGCTTCTACCAC
PRIMER_LEFT_17=372,20
PRIMER_RIGHT_17=471,20
PRIMER_LEFT_17_TM=59.797
PRIMER_RIGHT_17_TM=59.454
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=100
PRIMER_PAIR_17_PRODUCT_TM=74.0
PRIMER_PAIR_18_PENALTY=0.775672
PRIMER_LEFT_18_PENALTY=0.112124
PRIMER_RIGHT_18_PENALTY=0.663548
PRIMER_LEFT_18_SEQUENCE=CCAGATTGCCATGTTCTGTG
PRIMER_RIGHT_18_SEQUENCE=TTTTGGTCCCTGATGGATCT
PRIMER_LEFT_18=295,20
PRIMER_RIGHT_18=380,20
PRIMER_LEFT_18_TM=60.112
PRIMER_RIGHT_18_TM=59.336
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=86
PRIMER_PAIR_18_PRODUCT_TM=72.5
PRIMER_PAIR_19_PENALTY=0.874857
PRIMER_LEFT_19_PENALTY=0.466916
PRIMER_RIGHT_19_PENALTY=0.407941
PRIMER_LEFT_19_SEQUENCE=GCATTGATACCAAGGAAGGC
PRIMER_RIGHT_19_SEQUENCE=CTGGTTGGTTGGCTTCTACC
PRIMER_LEFT_19=384,20
PRIMER_RIGHT_19=473,20
PRIMER_LEFT_19_TM=59.533
PRIMER_RIGHT_19_TM=59.592
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=90
PRIMER_PAIR_19_PRODUCT_TM=73.3
PRIMER_PAIR_20_PENALTY=0.881003
PRIMER_LEFT_20_PENALTY=0.738752
PRIMER_RIGHT_20_PENALTY=0.142251
PRIMER_LEFT_20_SEQUENCE=CTCATCTTCACTGGCACACC
PRIMER_RIGHT_20_SEQUENCE=AACATGCCGTAGTCATGCAA
PRIMER_LEFT_20=636,20
PRIMER_RIGHT_20=711,20
PRIMER_LEFT_20_TM=59.261
PRIMER_RIGHT_20_TM=60.142
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=2.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=8.8000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=76
PRIMER_PAIR_20_PRODUCT_TM=72.5
PRIMER_PAIR_21_PENALTY=0.901425
PRIMER_LEFT_21_PENALTY=0.162673
PRIMER_RIGHT_21_PENALTY=0.738752
PRIMER_LEFT_21_SEQUENCE=CCCTTCTCGTTCCTGACAAG
PRIMER_RIGHT_21_SEQUENCE=GGTGTGCCAGTGAAGATGAG
PRIMER_LEFT_21=576,20
PRIMER_RIGHT_21=655,20
PRIMER_LEFT_21_TM=59.837
PRIMER_RIGHT_21_TM=59.261
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=3.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=80
PRIMER_PAIR_21_PRODUCT_TM=72.4
PRIMER_PAIR_22_PENALTY=0.953642
PRIMER_LEFT_22_PENALTY=0.554765
PRIMER_RIGHT_22_PENALTY=0.398877
PRIMER_LEFT_22_SEQUENCE=GAAGCCAACCAACCAGTGAC
PRIMER_RIGHT_22_SEQUENCE=CAACTAAGCAGCGGTAGGGA
PRIMER_LEFT_22=458,20
PRIMER_RIGHT_22=557,20
PRIMER_LEFT_22_TM=60.555
PRIMER_RIGHT_22_TM=60.399
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=3.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.1000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=100
PRIMER_PAIR_22_PRODUCT_TM=77.3
PRIMER_PAIR_23_PENALTY=1.005883
PRIMER_LEFT_23_PENALTY=0.532607
PRIMER_RIGHT_23_PENALTY=0.473276
PRIMER_LEFT_23_SEQUENCE=GCTGGAGGTACCCACTGATG
PRIMER_RIGHT_23_SEQUENCE=GCATGTTCAGTCTGCCACAG
PRIMER_LEFT_23=250,20
PRIMER_RIGHT_23=329,20
PRIMER_LEFT_23_TM=60.533
PRIMER_RIGHT_23_TM=60.473
PRIMER_LEFT_23_GC_PERCENT=60.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=3.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=80
PRIMER_PAIR_23_PRODUCT_TM=75.5
PRIMER_PAIR_24_PENALTY=1.153817
PRIMER_LEFT_24_PENALTY=0.546410
PRIMER_RIGHT_24_PENALTY=0.607407
PRIMER_LEFT_24_SEQUENCE=GTGGTAGAAGCCAACCAACC
PRIMER_RIGHT_24_SEQUENCE=ACAAAGTGGGGATGGGTCTT
PRIMER_LEFT_24=452,20
PRIMER_RIGHT_24=534,20
PRIMER_LEFT_24_TM=59.454
PRIMER_RIGHT_24_TM=60.607
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=83
PRIMER_PAIR_24_PRODUCT_TM=76.4
PRIMER_PAIR_25_PENALTY=1.160081
PRIMER_LEFT_25_PENALTY=0.906258
PRIMER_RIGHT_25_PENALTY=0.253823
PRIMER_LEFT_25_SEQUENCE=AGGAGAGGATGGATGTTTGC
PRIMER_RIGHT_25_SEQUENCE=CATGCCGTAGTCATGCAAGT
PRIMER_LEFT_25=612,20
PRIMER_RIGHT_25=709,20
PRIMER_LEFT_25_TM=59.094
PRIMER_RIGHT_25_TM=59.746
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=2.00
PRIMER_RIGHT_25_SELF_END=2.00
PRIMER_LEFT_25_END_STABILITY=8.8000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=98
PRIMER_PAIR_25_PRODUCT_TM=74.3
PRIMER_PAIR_26_PENALTY=1.200572
PRIMER_LEFT_26_PENALTY=0.607407
PRIMER_RIGHT_26_PENALTY=0.593166
PRIMER_LEFT_26_SEQUENCE=AAGACCCATCCCCACTTTGT
PRIMER_RIGHT_26_SEQUENCE=TGCACTTGTCAGGAACGAGA
PRIMER_LEFT_26=515,20
PRIMER_RIGHT_26=599,20
PRIMER_LEFT_26_TM=60.607
PRIMER_RIGHT_26_TM=60.593
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=85
PRIMER_PAIR_26_PRODUCT_TM=73.1
PRIMER_PAIR_27_PENALTY=1.201833
PRIMER_LEFT_27_PENALTY=0.598673
PRIMER_RIGHT_27_PENALTY=0.603160
PRIMER_LEFT_27_SEQUENCE=CCTACCGCTGCTTAGTTGGT
PRIMER_RIGHT_27_SEQUENCE=TTCGCAAACATCCATCCTCT
PRIMER_LEFT_27=540,20
PRIMER_RIGHT_27=634,20
PRIMER_LEFT_27_TM=59.401
PRIMER_RIGHT_27_TM=60.603
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=95
PRIMER_PAIR_27_PRODUCT_TM=73.0
PRIMER_PAIR_28_PENALTY=1.276794
PRIMER_LEFT_28_PENALTY=0.137602
PRIMER_RIGHT_28_PENALTY=1.139192
PRIMER_LEFT_28_SEQUENCE=GGAAGTGGGATTCAGATCCA
PRIMER_RIGHT_28_SEQUENCE=TTCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_28=348,20
PRIMER_RIGHT_28=436,21
PRIMER_LEFT_28_TM=59.862
PRIMER_RIGHT_28_TM=59.861
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=3.00
PRIMER_RIGHT_28_SELF_END=3.00
PRIMER_LEFT_28_END_STABILITY=8.1000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=89
PRIMER_PAIR_28_PRODUCT_TM=73.0
PRIMER_PAIR_29_PENALTY=1.292454
PRIMER_LEFT_29_PENALTY=0.839509
PRIMER_RIGHT_29_PENALTY=0.452945
PRIMER_LEFT_29_SEQUENCE=ACCAACCAGTGACCATCCAG
PRIMER_RIGHT_29_SEQUENCE=CTCACCAACTAAGCAGCGGT
PRIMER_LEFT_29=465,20
PRIMER_RIGHT_29=562,20
PRIMER_LEFT_29_TM=60.840
PRIMER_RIGHT_29_TM=60.453
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=2.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=11.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=98
PRIMER_PAIR_29_PRODUCT_TM=77.2
PRIMER_PAIR_30_PENALTY=1.321678
PRIMER_LEFT_30_PENALTY=0.327840
PRIMER_RIGHT_30_PENALTY=0.993838
PRIMER_LEFT_30_SEQUENCE=GTGGCAGACTGAACATGCAC
PRIMER_RIGHT_30_SEQUENCE=CAGGATGCCTTCCTTGGTAT
PRIMER_LEFT_30=312,20
PRIMER_RIGHT_30=409,20
PRIMER_LEFT_30_TM=60.328
PRIMER_RIGHT_30_TM=59.006
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=7.00
PRIMER_LEFT_30_SELF_END=2.00
PRIMER_RIGHT_30_SELF_END=2.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.8000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=98
PRIMER_PAIR_30_PRODUCT_TM=73.9
PRIMER_PAIR_31_PENALTY=1.325632
PRIMER_LEFT_31_PENALTY=1.188029
PRIMER_RIGHT_31_PENALTY=0.137602
PRIMER_LEFT_31_SEQUENCE=AACCCCAGATTGCCATGTT
PRIMER_RIGHT_31_SEQUENCE=GATCTGAATCCCACTTCCCA
PRIMER_LEFT_31=291,19
PRIMER_RIGHT_31=365,20
PRIMER_LEFT_31_TM=60.188
PRIMER_RIGHT_31_TM=59.862
PRIMER_LEFT_31_GC_PERCENT=47.368
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=2.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=75
PRIMER_PAIR_31_PRODUCT_TM=71.6
PRIMER_PAIR_32_PENALTY=1.421629
PRIMER_LEFT_32_PENALTY=0.689681
PRIMER_RIGHT_32_PENALTY=0.731948
PRIMER_LEFT_32_SEQUENCE=AGAAGCCAACCAACCAGTGA
PRIMER_RIGHT_32_SEQUENCE=TAAGCAGCGGTAGGGAATCA
PRIMER_LEFT_32=457,20
PRIMER_RIGHT_32=553,20
PRIMER_LEFT_32_TM=60.690
PRIMER_RIGHT_32_TM=60.732
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=97
PRIMER_PAIR_32_PRODUCT_TM=77.0
PRIMER_PAIR_33_PENALTY=1.426873
PRIMER_LEFT_33_PENALTY=0.426779
PRIMER_RIGHT_33_PENALTY=1.000094
PRIMER_LEFT_33_SEQUENCE=TCGTTCCTGACAAGTGCAAA
PRIMER_RIGHT_33_SEQUENCE=ACTGCATGTCTCTTTGGCG
PRIMER_LEFT_33=582,20
PRIMER_RIGHT_33=676,19
PRIMER_LEFT_33_TM=60.427
PRIMER_RIGHT_33_TM=60.000
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=52.632
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=2.00
PRIMER_LEFT_33_END_STABILITY=8.8000
PRIMER_RIGHT_33_END_STABILITY=11.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=95
PRIMER_PAIR_33_PRODUCT_TM=73.4
PRIMER_PAIR_34_PENALTY=1.429836
PRIMER_LEFT_34_PENALTY=0.079648
PRIMER_RIGHT_34_PENALTY=1.350189
PRIMER_LEFT_34_SEQUENCE=CACCAGGAGAGGATGGATGT
PRIMER_RIGHT_34_SEQUENCE=CCGTAGTCATGCAAGTTGGT
PRIMER_LEFT_34=608,20
PRIMER_RIGHT_34=705,20
PRIMER_LEFT_34_TM=59.920
PRIMER_RIGHT_34_TM=58.650
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.3000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=98
PRIMER_PAIR_34_PRODUCT_TM=74.7
PRIMER_PAIR_35_PENALTY=1.515297
PRIMER_LEFT_35_PENALTY=1.062353
PRIMER_RIGHT_35_PENALTY=0.452945
PRIMER_LEFT_35_SEQUENCE=CCAACCAGTGACCATCCAG
PRIMER_RIGHT_35_SEQUENCE=ACTCACCAACTAAGCAGCGG
PRIMER_LEFT_35=466,19
PRIMER_RIGHT_35=563,20
PRIMER_LEFT_35_TM=59.938
PRIMER_RIGHT_35_TM=60.453
PRIMER_LEFT_35_GC_PERCENT=57.895
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=2.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=11.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=98
PRIMER_PAIR_35_PRODUCT_TM=77.2
PRIMER_PAIR_36_PENALTY=1.554865
PRIMER_LEFT_36_PENALTY=0.473276
PRIMER_RIGHT_36_PENALTY=1.081589
PRIMER_LEFT_36_SEQUENCE=CTGTGGCAGACTGAACATGC
PRIMER_RIGHT_36_SEQUENCE=GCAGGTTTTGGTCCCTGAT
PRIMER_LEFT_36=310,20
PRIMER_RIGHT_36=385,19
PRIMER_LEFT_36_TM=60.473
PRIMER_RIGHT_36_TM=59.918
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=52.632
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=2.00
PRIMER_RIGHT_36_SELF_END=2.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=6.6000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=76
PRIMER_PAIR_36_PRODUCT_TM=72.5
PRIMER_PAIR_37_PENALTY=1.638206
PRIMER_LEFT_37_PENALTY=0.731948
PRIMER_RIGHT_37_PENALTY=0.906258
PRIMER_LEFT_37_SEQUENCE=TGATTCCCTACCGCTGCTTA
PRIMER_RIGHT_37_SEQUENCE=GCAAACATCCATCCTCTCCT
PRIMER_LEFT_37=534,20
PRIMER_RIGHT_37=631,20
PRIMER_LEFT_37_TM=60.732
PRIMER_RIGHT_37_TM=59.094
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=2.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.5000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=98
PRIMER_PAIR_37_PRODUCT_TM=73.0
PRIMER_PAIR_38_PENALTY=1.710319
PRIMER_LEFT_38_PENALTY=0.079648
PRIMER_RIGHT_38_PENALTY=1.630671
PRIMER_LEFT_38_SEQUENCE=ACACCAGGAGAGGATGGATG
PRIMER_RIGHT_38_SEQUENCE=GCCGTAGTCATGCAAGTTGG
PRIMER_LEFT_38=607,20
PRIMER_RIGHT_38=706,20
PRIMER_LEFT_38_TM=59.920
PRIMER_RIGHT_38_TM=61.631
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=3.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=100
PRIMER_PAIR_38_PRODUCT_TM=74.8
PRIMER_PAIR_39_PENALTY=1.727747
PRIMER_LEFT_39_PENALTY=1.215461
PRIMER_RIGHT_39_PENALTY=0.512286
PRIMER_LEFT_39_SEQUENCE=ACCAACCAGTGACCATCCA
PRIMER_RIGHT_39_SEQUENCE=TCACCAACTAAGCAGCGGTA
PRIMER_LEFT_39=465,19
PRIMER_RIGHT_39=561,20
PRIMER_LEFT_39_TM=59.785
PRIMER_RIGHT_39_TM=59.488
PRIMER_LEFT_39_GC_PERCENT=52.632
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=2.00
PRIMER_LEFT_39_END_STABILITY=8.1000
PRIMER_RIGHT_39_END_STABILITY=8.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=97
PRIMER_PAIR_39_PRODUCT_TM=77.0
PRIMER_PAIR_40_PENALTY=1.751977
PRIMER_LEFT_40_PENALTY=0.923785
PRIMER_RIGHT_40_PENALTY=0.828193
PRIMER_LEFT_40_SEQUENCE=CCCAGATTGCCATGTTCTGT
PRIMER_RIGHT_40_SEQUENCE=CCTGATGGATCTGAATCCCA
PRIMER_LEFT_40=294,20
PRIMER_RIGHT_40=372,20
PRIMER_LEFT_40_TM=60.924
PRIMER_RIGHT_40_TM=60.828
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=3.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=79
PRIMER_PAIR_40_PRODUCT_TM=72.5
PRIMER_PAIR_41_PENALTY=1.782319
PRIMER_LEFT_41_PENALTY=1.146286
PRIMER_RIGHT_41_PENALTY=0.636033
PRIMER_LEFT_41_SEQUENCE=ATCTTCACTGGCACACCGTC
PRIMER_RIGHT_41_SEQUENCE=GAACTTGTCAATTCCGCAGG
PRIMER_LEFT_41=639,20
PRIMER_RIGHT_41=736,20
PRIMER_LEFT_41_TM=61.146
PRIMER_RIGHT_41_TM=60.636
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=7.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=2.00
PRIMER_LEFT_41_END_STABILITY=9.6000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=98
PRIMER_PAIR_41_PRODUCT_TM=74.7
PRIMER_PAIR_42_PENALTY=1.821407
PRIMER_LEFT_42_PENALTY=1.683804
PRIMER_RIGHT_42_PENALTY=0.137602
PRIMER_LEFT_42_SEQUENCE=ACCCCAGATTGCCATGTTCT
PRIMER_RIGHT_42_SEQUENCE=TGGATCTGAATCCCACTTCC
PRIMER_LEFT_42=292,20
PRIMER_RIGHT_42=367,20
PRIMER_LEFT_42_TM=61.684
PRIMER_RIGHT_42_TM=59.862
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=2.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=76
PRIMER_PAIR_42_PRODUCT_TM=72.0
PRIMER_PAIR_43_PENALTY=1.836755
PRIMER_LEFT_43_PENALTY=1.238082
PRIMER_RIGHT_43_PENALTY=0.598673
PRIMER_LEFT_43_SEQUENCE=AACCAACCAGTGACCATCCA
PRIMER_RIGHT_43_SEQUENCE=ACCAACTAAGCAGCGGTAGG
PRIMER_LEFT_43=464,20
PRIMER_RIGHT_43=559,20
PRIMER_LEFT_43_TM=61.238
PRIMER_RIGHT_43_TM=59.401
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=96
PRIMER_PAIR_43_PRODUCT_TM=76.7
PRIMER_PAIR_44_PENALTY=1.858077
PRIMER_LEFT_44_PENALTY=0.711791
PRIMER_RIGHT_44_PENALTY=1.146286
PRIMER_LEFT_44_SEQUENCE=AAGTGATGCCCTTCTCGTTC
PRIMER_RIGHT_44_SEQUENCE=GACGGTGTGCCAGTGAAGAT
PRIMER_LEFT_44=568,20
PRIMER_RIGHT_44=658,20
PRIMER_LEFT_44_TM=59.288
PRIMER_RIGHT_44_TM=61.146
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=2.00
PRIMER_LEFT_44_END_STABILITY=8.4000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=91
PRIMER_PAIR_44_PRODUCT_TM=73.1
PRIMER_PAIR_45_PENALTY=1.870574
PRIMER_LEFT_45_PENALTY=0.805475
PRIMER_RIGHT_45_PENALTY=1.065099
PRIMER_LEFT_45_SEQUENCE=CTTTGTGATTCCCTACCGCT
PRIMER_RIGHT_45_SEQUENCE=CATCCATCCTCTCCTGGTGTA
PRIMER_LEFT_45=529,20
PRIMER_RIGHT_45=626,21
PRIMER_LEFT_45_TM=59.195
PRIMER_RIGHT_45_TM=59.935
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=2.00
PRIMER_RIGHT_45_SELF_END=2.00
PRIMER_LEFT_45_END_STABILITY=11.4000
PRIMER_RIGHT_45_END_STABILITY=5.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=98
PRIMER_PAIR_45_PRODUCT_TM=73.0
PRIMER_PAIR_46_PENALTY=1.873083
PRIMER_LEFT_46_PENALTY=1.392601
PRIMER_RIGHT_46_PENALTY=0.480482
PRIMER_LEFT_46_SEQUENCE=TCCTGCAGTATTGCCAAGAAG
PRIMER_RIGHT_46_SEQUENCE=TTCTGGATGGTCACTGGTTG
PRIMER_LEFT_46=405,21
PRIMER_RIGHT_46=486,20
PRIMER_LEFT_46_TM=60.393
PRIMER_RIGHT_46_TM=59.520
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=82
PRIMER_PAIR_46_PRODUCT_TM=72.6
PRIMER_PAIR_47_PENALTY=1.939458
PRIMER_LEFT_47_PENALTY=0.949861
PRIMER_RIGHT_47_PENALTY=0.989597
PRIMER_LEFT_47_SEQUENCE=ATCCCCACTTTGTGATTCCC
PRIMER_RIGHT_47_SEQUENCE=GCACTTGTCAGGAACGAGAA
PRIMER_LEFT_47=522,20
PRIMER_RIGHT_47=598,20
PRIMER_LEFT_47_TM=60.950
PRIMER_RIGHT_47_TM=59.010
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=3.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=77
PRIMER_PAIR_47_PRODUCT_TM=72.3
PRIMER_PAIR_48_PENALTY=1.982572
PRIMER_LEFT_48_PENALTY=1.427808
PRIMER_RIGHT_48_PENALTY=0.554765
PRIMER_LEFT_48_SEQUENCE=TGCATTGATACCAAGGAAGG
PRIMER_RIGHT_48_SEQUENCE=GTCACTGGTTGGTTGGCTTC
PRIMER_LEFT_48=383,20
PRIMER_RIGHT_48=477,20
PRIMER_LEFT_48_TM=58.572
PRIMER_RIGHT_48_TM=60.555
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=95
PRIMER_PAIR_48_PRODUCT_TM=73.4
PRIMER_PAIR_49_PENALTY=1.985862
PRIMER_LEFT_49_PENALTY=1.003259
PRIMER_RIGHT_49_PENALTY=0.982603
PRIMER_LEFT_49_SEQUENCE=TTCCCTACCGCTGCTTAGTT
PRIMER_RIGHT_49_SEQUENCE=TTTCGCAAACATCCATCCTC
PRIMER_LEFT_49=537,20
PRIMER_RIGHT_49=635,20
PRIMER_LEFT_49_TM=58.997
PRIMER_RIGHT_49_TM=60.983
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.7000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=99
PRIMER_PAIR_49_PRODUCT_TM=72.9
PRIMER_PAIR_50_PENALTY=1.999512
PRIMER_LEFT_50_PENALTY=1.126243
PRIMER_RIGHT_50_PENALTY=0.873270
PRIMER_LEFT_50_SEQUENCE=TGTAAGTGATGCCCTTCTCG
PRIMER_RIGHT_50_SEQUENCE=TGAGTTTCGCAAACATCCAT
PRIMER_LEFT_50=565,20
PRIMER_RIGHT_50=639,20
PRIMER_LEFT_50_TM=58.874
PRIMER_RIGHT_50_TM=59.127
PRIMER_LEFT_50_GC_PERCENT=50.000
PRIMER_RIGHT_50_GC_PERCENT=40.000
PRIMER_LEFT_50_SELF_ANY=4.00
PRIMER_RIGHT_50_SELF_ANY=6.00
PRIMER_LEFT_50_SELF_END=2.00
PRIMER_RIGHT_50_SELF_END=2.00
PRIMER_LEFT_50_END_STABILITY=8.4000
PRIMER_RIGHT_50_END_STABILITY=8.1000
PRIMER_PAIR_50_COMPL_ANY=4.00
PRIMER_PAIR_50_COMPL_END=1.00
PRIMER_PAIR_50_PRODUCT_SIZE=75
PRIMER_PAIR_50_PRODUCT_TM=70.5
PRIMER_PAIR_51_PENALTY=2.005671
PRIMER_LEFT_51_PENALTY=1.055809
PRIMER_RIGHT_51_PENALTY=0.949861
PRIMER_LEFT_51_SEQUENCE=CCCAGATTGCCATGTTCTG
PRIMER_RIGHT_51_SEQUENCE=GGTTTTGGTCCCTGATGGAT
PRIMER_LEFT_51=294,19
PRIMER_RIGHT_51=382,20
PRIMER_LEFT_51_TM=60.056
PRIMER_RIGHT_51_TM=60.950
PRIMER_LEFT_51_GC_PERCENT=52.632
PRIMER_RIGHT_51_GC_PERCENT=50.000
PRIMER_LEFT_51_SELF_ANY=4.00
PRIMER_RIGHT_51_SELF_ANY=5.00
PRIMER_LEFT_51_SELF_END=1.00
PRIMER_RIGHT_51_SELF_END=3.00
PRIMER_LEFT_51_END_STABILITY=7.0000
PRIMER_RIGHT_51_END_STABILITY=8.1000
PRIMER_PAIR_51_COMPL_ANY=4.00
PRIMER_PAIR_51_COMPL_END=0.00
PRIMER_PAIR_51_PRODUCT_SIZE=89
PRIMER_PAIR_51_PRODUCT_TM=73.4
PRIMER_PAIR_52_PENALTY=2.032732
PRIMER_LEFT_52_PENALTY=1.227257
PRIMER_RIGHT_52_PENALTY=0.805475
PRIMER_LEFT_52_SEQUENCE=TAGAAGCCAACCAACCAGTG
PRIMER_RIGHT_52_SEQUENCE=AGCGGTAGGGAATCACAAAG
PRIMER_LEFT_52=456,20
PRIMER_RIGHT_52=548,20
PRIMER_LEFT_52_TM=58.773
PRIMER_RIGHT_52_TM=59.195
PRIMER_LEFT_52_GC_PERCENT=50.000
PRIMER_RIGHT_52_GC_PERCENT=50.000
PRIMER_LEFT_52_SELF_ANY=3.00
PRIMER_RIGHT_52_SELF_ANY=3.00
PRIMER_LEFT_52_SELF_END=3.00
PRIMER_RIGHT_52_SELF_END=0.00
PRIMER_LEFT_52_END_STABILITY=6.7000
PRIMER_RIGHT_52_END_STABILITY=7.3000
PRIMER_PAIR_52_COMPL_ANY=4.00
PRIMER_PAIR_52_COMPL_END=1.00
PRIMER_PAIR_52_PRODUCT_SIZE=93
PRIMER_PAIR_52_PRODUCT_TM=76.8
PRIMER_PAIR_53_PENALTY=2.041918
PRIMER_LEFT_53_PENALTY=1.139192
PRIMER_RIGHT_53_PENALTY=0.902727
PRIMER_LEFT_53_SEQUENCE=TGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_53_SEQUENCE=CCAGTTCTGGATGGTCACTG
PRIMER_LEFT_53=416,21
PRIMER_RIGHT_53=490,20
PRIMER_LEFT_53_TM=59.861
PRIMER_RIGHT_53_TM=59.097
PRIMER_LEFT_53_GC_PERCENT=47.619
PRIMER_RIGHT_53_GC_PERCENT=55.000
PRIMER_LEFT_53_SELF_ANY=4.00
PRIMER_RIGHT_53_SELF_ANY=6.00
PRIMER_LEFT_53_SELF_END=0.00
PRIMER_RIGHT_53_SELF_END=3.00
PRIMER_LEFT_53_END_STABILITY=7.0000
PRIMER_RIGHT_53_END_STABILITY=6.7000
PRIMER_PAIR_53_COMPL_ANY=4.00
PRIMER_PAIR_53_COMPL_END=2.00
PRIMER_PAIR_53_PRODUCT_SIZE=75
PRIMER_PAIR_53_PRODUCT_TM=72.7
PRIMER_PAIR_54_PENALTY=2.041918
PRIMER_LEFT_54_PENALTY=1.139192
PRIMER_RIGHT_54_PENALTY=0.902727
PRIMER_LEFT_54_SEQUENCE=TTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_54_SEQUENCE=CAGTTCTGGATGGTCACTGG
PRIMER_LEFT_54=415,21
PRIMER_RIGHT_54=489,20
PRIMER_LEFT_54_TM=59.861
PRIMER_RIGHT_54_TM=59.097
PRIMER_LEFT_54_GC_PERCENT=47.619
PRIMER_RIGHT_54_GC_PERCENT=55.000
PRIMER_LEFT_54_SELF_ANY=5.00
PRIMER_RIGHT_54_SELF_ANY=4.00
PRIMER_LEFT_54_SELF_END=1.00
PRIMER_RIGHT_54_SELF_END=1.00
PRIMER_LEFT_54_END_STABILITY=8.2000
PRIMER_RIGHT_54_END_STABILITY=7.9000
PRIMER_PAIR_54_COMPL_ANY=4.00
PRIMER_PAIR_54_COMPL_END=2.00
PRIMER_PAIR_54_PRODUCT_SIZE=75
PRIMER_PAIR_54_PRODUCT_TM=72.1
PRIMER_PAIR_55_PENALTY=2.053793
PRIMER_LEFT_55_PENALTY=1.950354
PRIMER_RIGHT_55_PENALTY=0.103439
PRIMER_LEFT_55_SEQUENCE=GTCGCCAAAGAGACATGCAG
PRIMER_RIGHT_55_SEQUENCE=CACAAACTCTACCCCTCGGA
PRIMER_LEFT_55=656,20
PRIMER_RIGHT_55=754,20
PRIMER_LEFT_55_TM=61.950
PRIMER_RIGHT_55_TM=60.103
PRIMER_LEFT_55_GC_PERCENT=55.000
PRIMER_RIGHT_55_GC_PERCENT=55.000
PRIMER_LEFT_55_SELF_ANY=4.00
PRIMER_RIGHT_55_SELF_ANY=3.00
PRIMER_LEFT_55_SELF_END=2.00
PRIMER_RIGHT_55_SELF_END=3.00
PRIMER_LEFT_55_END_STABILITY=8.5000
PRIMER_RIGHT_55_END_STABILITY=9.9000
PRIMER_PAIR_55_COMPL_ANY=4.00
PRIMER_PAIR_55_COMPL_END=1.00
PRIMER_PAIR_55_PRODUCT_SIZE=99
PRIMER_PAIR_55_PRODUCT_TM=75.0
PRIMER_PAIR_56_PENALTY=2.080242
PRIMER_LEFT_56_PENALTY=1.020705
PRIMER_RIGHT_56_PENALTY=1.059537
PRIMER_LEFT_56_SEQUENCE=TTTGTGATTCCCTACCGCTG
PRIMER_RIGHT_56_SEQUENCE=AAACATCCATCCTCTCCTGG
PRIMER_LEFT_56=530,20
PRIMER_RIGHT_56=629,20
PRIMER_LEFT_56_TM=61.021
PRIMER_RIGHT_56_TM=58.940
PRIMER_LEFT_56_GC_PERCENT=50.000
PRIMER_RIGHT_56_GC_PERCENT=50.000
PRIMER_LEFT_56_SELF_ANY=3.00
PRIMER_RIGHT_56_SELF_ANY=3.00
PRIMER_LEFT_56_SELF_END=2.00
PRIMER_RIGHT_56_SELF_END=3.00
PRIMER_LEFT_56_END_STABILITY=10.2000
PRIMER_RIGHT_56_END_STABILITY=9.7000
PRIMER_PAIR_56_COMPL_ANY=3.00
PRIMER_PAIR_56_COMPL_END=0.00
PRIMER_PAIR_56_PRODUCT_SIZE=100
PRIMER_PAIR_56_PRODUCT_TM=72.4
PRIMER_PAIR_57_PENALTY=2.085972
PRIMER_LEFT_57_PENALTY=1.071784
PRIMER_RIGHT_57_PENALTY=1.014189
PRIMER_LEFT_57_SEQUENCE=GATTGCCATGTTCTGTGGC
PRIMER_RIGHT_57_SEQUENCE=TGGTCCCTGATGGATCTGA
PRIMER_LEFT_57=298,19
PRIMER_RIGHT_57=377,19
PRIMER_LEFT_57_TM=60.072
PRIMER_RIGHT_57_TM=59.986
PRIMER_LEFT_57_GC_PERCENT=52.632
PRIMER_RIGHT_57_GC_PERCENT=52.632
PRIMER_LEFT_57_SELF_ANY=4.00
PRIMER_RIGHT_57_SELF_ANY=5.00
PRIMER_LEFT_57_SELF_END=2.00
PRIMER_RIGHT_57_SELF_END=3.00
PRIMER_LEFT_57_END_STABILITY=9.4000
PRIMER_RIGHT_57_END_STABILITY=6.7000
PRIMER_PAIR_57_COMPL_ANY=4.00
PRIMER_PAIR_57_COMPL_END=0.00
PRIMER_PAIR_57_PRODUCT_SIZE=80
PRIMER_PAIR_57_PRODUCT_TM=72.4
PRIMER_PAIR_58_PENALTY=2.105553
PRIMER_LEFT_58_PENALTY=0.944463
PRIMER_RIGHT_58_PENALTY=1.161090
PRIMER_LEFT_58_SEQUENCE=ACTTTGTGATTCCCTACCGC
PRIMER_RIGHT_58_SEQUENCE=AATTTGCACTTGTCAGGAACG
PRIMER_LEFT_58=528,20
PRIMER_RIGHT_58=603,21
PRIMER_LEFT_58_TM=59.056
PRIMER_RIGHT_58_TM=60.161
PRIMER_LEFT_58_GC_PERCENT=50.000
PRIMER_RIGHT_58_GC_PERCENT=42.857
PRIMER_LEFT_58_SELF_ANY=3.00
PRIMER_RIGHT_58_SELF_ANY=4.00
PRIMER_LEFT_58_SELF_END=2.00
PRIMER_RIGHT_58_SELF_END=2.00
PRIMER_LEFT_58_END_STABILITY=11.1000
PRIMER_RIGHT_58_END_STABILITY=8.4000
PRIMER_PAIR_58_COMPL_ANY=4.00
PRIMER_PAIR_58_COMPL_END=1.00
PRIMER_PAIR_58_PRODUCT_SIZE=76
PRIMER_PAIR_58_PRODUCT_TM=70.4
PRIMER_PAIR_59_PENALTY=2.131956
PRIMER_LEFT_59_PENALTY=1.468408
PRIMER_RIGHT_59_PENALTY=0.663548
PRIMER_LEFT_59_SEQUENCE=CCACTGATGGTAATGCTGGC
PRIMER_RIGHT_59_SEQUENCE=GAATCCCACTTCCCATTCTG
PRIMER_LEFT_59=261,20
PRIMER_RIGHT_59=360,20
PRIMER_LEFT_59_TM=61.468
PRIMER_RIGHT_59_TM=59.336
PRIMER_LEFT_59_GC_PERCENT=55.000
PRIMER_RIGHT_59_GC_PERCENT=50.000
PRIMER_LEFT_59_SELF_ANY=3.00
PRIMER_RIGHT_59_SELF_ANY=4.00
PRIMER_LEFT_59_SELF_END=2.00
PRIMER_RIGHT_59_SELF_END=1.00
PRIMER_LEFT_59_END_STABILITY=9.7000
PRIMER_RIGHT_59_END_STABILITY=7.0000
PRIMER_PAIR_59_COMPL_ANY=5.00
PRIMER_PAIR_59_COMPL_END=3.00
PRIMER_PAIR_59_PRODUCT_SIZE=100
PRIMER_PAIR_59_PRODUCT_TM=75.2
PRIMER_PAIR_60_PENALTY=2.164052
PRIMER_LEFT_60_PENALTY=0.989597
PRIMER_RIGHT_60_PENALTY=1.174455
PRIMER_LEFT_60_SEQUENCE=TTCTCGTTCCTGACAAGTGC
PRIMER_RIGHT_60_SEQUENCE=GGTGTGCCAGTGAAGATGAGT
PRIMER_LEFT_60=579,20
PRIMER_RIGHT_60=655,21
PRIMER_LEFT_60_TM=59.010
PRIMER_RIGHT_60_TM=60.174
PRIMER_LEFT_60_GC_PERCENT=50.000
PRIMER_RIGHT_60_GC_PERCENT=52.381
PRIMER_LEFT_60_SELF_ANY=3.00
PRIMER_RIGHT_60_SELF_ANY=3.00
PRIMER_LEFT_60_SELF_END=2.00
PRIMER_RIGHT_60_SELF_END=1.00
PRIMER_LEFT_60_END_STABILITY=7.9000
PRIMER_RIGHT_60_END_STABILITY=6.4000
PRIMER_PAIR_60_COMPL_ANY=5.00
PRIMER_PAIR_60_COMPL_END=0.00
PRIMER_PAIR_60_PRODUCT_SIZE=77
PRIMER_PAIR_60_PRODUCT_TM=71.3
PRIMER_PAIR_61_PENALTY=2.182545
PRIMER_LEFT_61_PENALTY=1.238082
PRIMER_RIGHT_61_PENALTY=0.944463
PRIMER_LEFT_61_SEQUENCE=CAACCAACCAGTGACCATCC
PRIMER_RIGHT_61_SEQUENCE=GCGGTAGGGAATCACAAAGT
PRIMER_LEFT_61=463,20
PRIMER_RIGHT_61=547,20
PRIMER_LEFT_61_TM=61.238
PRIMER_RIGHT_61_TM=59.056
PRIMER_LEFT_61_GC_PERCENT=55.000
PRIMER_RIGHT_61_GC_PERCENT=50.000
PRIMER_LEFT_61_SELF_ANY=3.00
PRIMER_RIGHT_61_SELF_ANY=3.00
PRIMER_LEFT_61_SELF_END=0.00
PRIMER_RIGHT_61_SELF_END=1.00
PRIMER_LEFT_61_END_STABILITY=8.1000
PRIMER_RIGHT_61_END_STABILITY=6.7000
PRIMER_PAIR_61_COMPL_ANY=4.00
PRIMER_PAIR_61_COMPL_END=0.00
PRIMER_PAIR_61_PRODUCT_SIZE=85
PRIMER_PAIR_61_PRODUCT_TM=77.0
PRIMER_PAIR_62_PENALTY=2.196647
PRIMER_LEFT_62_PENALTY=1.153556
PRIMER_RIGHT_62_PENALTY=1.043090
PRIMER_LEFT_62_SEQUENCE=CAAGCAGTGCAAGACCCAT
PRIMER_RIGHT_62_SEQUENCE=GCACTTGTCAGGAACGAGAAG
PRIMER_LEFT_62=505,19
PRIMER_RIGHT_62=598,21
PRIMER_LEFT_62_TM=59.846
PRIMER_RIGHT_62_TM=60.043
PRIMER_LEFT_62_GC_PERCENT=52.632
PRIMER_RIGHT_62_GC_PERCENT=52.381
PRIMER_LEFT_62_SELF_ANY=5.00
PRIMER_RIGHT_62_SELF_ANY=4.00
PRIMER_LEFT_62_SELF_END=2.00
PRIMER_RIGHT_62_SELF_END=3.00
PRIMER_LEFT_62_END_STABILITY=9.6000
PRIMER_RIGHT_62_END_STABILITY=6.7000
PRIMER_PAIR_62_COMPL_ANY=5.00
PRIMER_PAIR_62_COMPL_END=1.00
PRIMER_PAIR_62_PRODUCT_SIZE=94
PRIMER_PAIR_62_PRODUCT_TM=74.5
PRIMER_PAIR_63_PENALTY=2.207651
PRIMER_LEFT_63_PENALTY=1.048185
PRIMER_RIGHT_63_PENALTY=1.159466
PRIMER_LEFT_63_SEQUENCE=GATTCCCTACCGCTGCTTAG
PRIMER_RIGHT_63_SEQUENCE=TCGCAAACATCCATCCTCTC
PRIMER_LEFT_63=535,20
PRIMER_RIGHT_63=633,20
PRIMER_LEFT_63_TM=58.952
PRIMER_RIGHT_63_TM=61.159
PRIMER_LEFT_63_GC_PERCENT=55.000
PRIMER_RIGHT_63_GC_PERCENT=50.000
PRIMER_LEFT_63_SELF_ANY=3.00
PRIMER_RIGHT_63_SELF_ANY=2.00
PRIMER_LEFT_63_SELF_END=3.00
PRIMER_RIGHT_63_SELF_END=0.00
PRIMER_LEFT_63_END_STABILITY=6.0000
PRIMER_RIGHT_63_END_STABILITY=6.4000
PRIMER_PAIR_63_COMPL_ANY=3.00
PRIMER_PAIR_63_COMPL_END=1.00
PRIMER_PAIR_63_PRODUCT_SIZE=99
PRIMER_PAIR_63_PRODUCT_TM=73.3
PRIMER_PAIR_64_PENALTY=2.221779
PRIMER_LEFT_64_PENALTY=0.663548
PRIMER_RIGHT_64_PENALTY=1.558232
PRIMER_LEFT_64_SEQUENCE=AGATCCATCAGGGACCAAAA
PRIMER_RIGHT_64_SEQUENCE=TCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_64=361,20
PRIMER_RIGHT_64=435,20
PRIMER_LEFT_64_TM=59.336
PRIMER_RIGHT_64_TM=58.442
PRIMER_LEFT_64_GC_PERCENT=45.000
PRIMER_RIGHT_64_GC_PERCENT=50.000
PRIMER_LEFT_64_SELF_ANY=5.00
PRIMER_RIGHT_64_SELF_ANY=4.00
PRIMER_LEFT_64_SELF_END=0.00
PRIMER_RIGHT_64_SELF_END=3.00
PRIMER_LEFT_64_END_STABILITY=7.6000
PRIMER_RIGHT_64_END_STABILITY=10.0000
PRIMER_PAIR_64_COMPL_ANY=4.00
PRIMER_PAIR_64_COMPL_END=2.00
PRIMER_PAIR_64_PRODUCT_SIZE=75
PRIMER_PAIR_64_PRODUCT_TM=71.6
PRIMER_PAIR_65_PENALTY=2.236534
PRIMER_LEFT_65_PENALTY=1.011619
PRIMER_RIGHT_65_PENALTY=1.224915
PRIMER_LEFT_65_SEQUENCE=ACTTTGTGATTCCCTACCGCT
PRIMER_RIGHT_65_SEQUENCE=ATTTGCACTTGTCAGGAACG
PRIMER_LEFT_65=528,21
PRIMER_RIGHT_65=602,20
PRIMER_LEFT_65_TM=60.012
PRIMER_RIGHT_65_TM=58.775
PRIMER_LEFT_65_GC_PERCENT=47.619
PRIMER_RIGHT_65_GC_PERCENT=45.000
PRIMER_LEFT_65_SELF_ANY=3.00
PRIMER_RIGHT_65_SELF_ANY=4.00
PRIMER_LEFT_65_SELF_END=2.00
PRIMER_RIGHT_65_SELF_END=2.00
PRIMER_LEFT_65_END_STABILITY=11.4000
PRIMER_RIGHT_65_END_STABILITY=8.4000
PRIMER_PAIR_65_COMPL_ANY=4.00
PRIMER_PAIR_65_COMPL_END=2.00
PRIMER_PAIR_65_PRODUCT_SIZE=75
PRIMER_PAIR_65_PRODUCT_TM=70.5
PRIMER_PAIR_66_PENALTY=2.241341
PRIMER_LEFT_66_PENALTY=1.238082
PRIMER_RIGHT_66_PENALTY=1.003259
PRIMER_LEFT_66_SEQUENCE=CCAACCAACCAGTGACCATC
PRIMER_RIGHT_66_SEQUENCE=AACTAAGCAGCGGTAGGGAA
PRIMER_LEFT_66=462,20
PRIMER_RIGHT_66=556,20
PRIMER_LEFT_66_TM=61.238
PRIMER_RIGHT_66_TM=58.997
PRIMER_LEFT_66_GC_PERCENT=55.000
PRIMER_RIGHT_66_GC_PERCENT=50.000
PRIMER_LEFT_66_SELF_ANY=3.00
PRIMER_RIGHT_66_SELF_ANY=3.00
PRIMER_LEFT_66_SELF_END=1.00
PRIMER_RIGHT_66_SELF_END=0.00
PRIMER_LEFT_66_END_STABILITY=8.1000
PRIMER_RIGHT_66_END_STABILITY=9.7000
PRIMER_PAIR_66_COMPL_ANY=4.00
PRIMER_PAIR_66_COMPL_END=1.00
PRIMER_PAIR_66_PRODUCT_SIZE=95
PRIMER_PAIR_66_PRODUCT_TM=76.9
PRIMER_PAIR_67_PENALTY=2.261575
PRIMER_LEFT_67_PENALTY=1.078278
PRIMER_RIGHT_67_PENALTY=1.183296
PRIMER_LEFT_67_SEQUENCE=AGATTGCCATGTTCTGTGGC
PRIMER_RIGHT_67_SEQUENCE=GTTTTGGTCCCTGATGGATCT
PRIMER_LEFT_67=297,20
PRIMER_RIGHT_67=381,21
PRIMER_LEFT_67_TM=61.078
PRIMER_RIGHT_67_TM=60.183
PRIMER_LEFT_67_GC_PERCENT=50.000
PRIMER_RIGHT_67_GC_PERCENT=47.619
PRIMER_LEFT_67_SELF_ANY=4.00
PRIMER_RIGHT_67_SELF_ANY=5.00
PRIMER_LEFT_67_SELF_END=2.00
PRIMER_RIGHT_67_SELF_END=3.00
PRIMER_LEFT_67_END_STABILITY=9.4000
PRIMER_RIGHT_67_END_STABILITY=6.3000
PRIMER_PAIR_67_COMPL_ANY=4.00
PRIMER_PAIR_67_COMPL_END=0.00
PRIMER_PAIR_67_PRODUCT_SIZE=85
PRIMER_PAIR_67_PRODUCT_TM=72.1
PRIMER_PAIR_68_PENALTY=2.267317
PRIMER_LEFT_68_PENALTY=1.427808
PRIMER_RIGHT_68_PENALTY=0.839509
PRIMER_LEFT_68_SEQUENCE=CATTGATACCAAGGAAGGCA
PRIMER_RIGHT_68_SEQUENCE=CTGGATGGTCACTGGTTGGT
PRIMER_LEFT_68=385,20
PRIMER_RIGHT_68=484,20
PRIMER_LEFT_68_TM=58.572
PRIMER_RIGHT_68_TM=60.840
PRIMER_LEFT_68_GC_PERCENT=45.000
PRIMER_RIGHT_68_GC_PERCENT=55.000
PRIMER_LEFT_68_SELF_ANY=6.00
PRIMER_RIGHT_68_SELF_ANY=3.00
PRIMER_LEFT_68_SELF_END=0.00
PRIMER_RIGHT_68_SELF_END=0.00
PRIMER_LEFT_68_END_STABILITY=9.7000
PRIMER_RIGHT_68_END_STABILITY=8.2000
PRIMER_PAIR_68_COMPL_ANY=5.00
PRIMER_PAIR_68_COMPL_END=2.00
PRIMER_PAIR_68_PRODUCT_SIZE=100
PRIMER_PAIR_68_PRODUCT_TM=74.0
PRIMER_PAIR_69_PENALTY=2.343809
PRIMER_LEFT_69_PENALTY=1.236064
PRIMER_RIGHT_69_PENALTY=1.107745
PRIMER_LEFT_69_SEQUENCE=AAGACCCATCCCCACTTTG
PRIMER_RIGHT_69_SEQUENCE=CACTTGTCAGGAACGAGAAGG
PRIMER_LEFT_69=515,19
PRIMER_RIGHT_69=597,21
PRIMER_LEFT_69_TM=59.764
PRIMER_RIGHT_69_TM=59.892
PRIMER_LEFT_69_GC_PERCENT=52.632
PRIMER_RIGHT_69_GC_PERCENT=52.381
PRIMER_LEFT_69_SELF_ANY=3.00
PRIMER_RIGHT_69_SELF_ANY=4.00
PRIMER_LEFT_69_SELF_END=1.00
PRIMER_RIGHT_69_SELF_END=1.00
PRIMER_LEFT_69_END_STABILITY=7.3000
PRIMER_RIGHT_69_END_STABILITY=8.2000
PRIMER_PAIR_69_COMPL_ANY=3.00
PRIMER_PAIR_69_COMPL_END=1.00
PRIMER_PAIR_69_PRODUCT_SIZE=83
PRIMER_PAIR_69_PRODUCT_TM=72.9
PRIMER_PAIR_70_PENALTY=2.345473
PRIMER_LEFT_70_PENALTY=1.333854
PRIMER_RIGHT_70_PENALTY=1.011619
PRIMER_LEFT_70_SEQUENCE=GAAGCCAACCAACCAGTGA
PRIMER_RIGHT_70_SEQUENCE=AGCGGTAGGGAATCACAAAGT
PRIMER_LEFT_70=458,19
PRIMER_RIGHT_70=548,21
PRIMER_LEFT_70_TM=59.666
PRIMER_RIGHT_70_TM=60.012
PRIMER_LEFT_70_GC_PERCENT=52.632
PRIMER_RIGHT_70_GC_PERCENT=47.619
PRIMER_LEFT_70_SELF_ANY=3.00
PRIMER_RIGHT_70_SELF_ANY=3.00
PRIMER_LEFT_70_SELF_END=1.00
PRIMER_RIGHT_70_SELF_END=1.00
PRIMER_LEFT_70_END_STABILITY=6.4000
PRIMER_RIGHT_70_END_STABILITY=6.7000
PRIMER_PAIR_70_COMPL_ANY=4.00
PRIMER_PAIR_70_COMPL_END=2.00
PRIMER_PAIR_70_PRODUCT_SIZE=91
PRIMER_PAIR_70_PRODUCT_TM=77.2
PRIMER_PAIR_71_PENALTY=2.373120
PRIMER_LEFT_71_PENALTY=1.352415
PRIMER_RIGHT_71_PENALTY=1.020705
PRIMER_LEFT_71_SEQUENCE=GTAGAAGCCAACCAACCAGTG
PRIMER_RIGHT_71_SEQUENCE=CAGCGGTAGGGAATCACAAA
PRIMER_LEFT_71=455,21
PRIMER_RIGHT_71=549,20
PRIMER_LEFT_71_TM=59.648
PRIMER_RIGHT_71_TM=61.021
PRIMER_LEFT_71_GC_PERCENT=52.381
PRIMER_RIGHT_71_GC_PERCENT=50.000
PRIMER_LEFT_71_SELF_ANY=3.00
PRIMER_RIGHT_71_SELF_ANY=3.00
PRIMER_LEFT_71_SELF_END=3.00
PRIMER_RIGHT_71_SELF_END=1.00
PRIMER_LEFT_71_END_STABILITY=6.7000
PRIMER_RIGHT_71_END_STABILITY=7.0000
PRIMER_PAIR_71_COMPL_ANY=4.00
PRIMER_PAIR_71_COMPL_END=0.00
PRIMER_PAIR_71_PRODUCT_SIZE=95
PRIMER_PAIR_71_PRODUCT_TM=77.3
PRIMER_PAIR_72_PENALTY=2.402627
PRIMER_LEFT_72_PENALTY=1.741437
PRIMER_RIGHT_72_PENALTY=0.661190
PRIMER_LEFT_72_SEQUENCE=ACCCCAGATTGCCATGTTC
PRIMER_RIGHT_72_SEQUENCE=GGATCTGAATCCCACTTCCC
PRIMER_LEFT_72=292,19
PRIMER_RIGHT_72=366,20
PRIMER_LEFT_72_TM=60.741
PRIMER_RIGHT_72_TM=60.661
PRIMER_LEFT_72_GC_PERCENT=52.632
PRIMER_RIGHT_72_GC_PERCENT=55.000
PRIMER_LEFT_72_SELF_ANY=4.00
PRIMER_RIGHT_72_SELF_ANY=5.00
PRIMER_LEFT_72_SELF_END=0.00
PRIMER_RIGHT_72_SELF_END=0.00
PRIMER_LEFT_72_END_STABILITY=6.7000
PRIMER_RIGHT_72_END_STABILITY=9.7000
PRIMER_PAIR_72_COMPL_ANY=5.00
PRIMER_PAIR_72_COMPL_END=0.00
PRIMER_PAIR_72_PRODUCT_SIZE=75
PRIMER_PAIR_72_PRODUCT_TM=72.1
PRIMER_PAIR_73_PENALTY=2.417959
PRIMER_LEFT_73_PENALTY=1.672380
PRIMER_RIGHT_73_PENALTY=0.745579
PRIMER_LEFT_73_SEQUENCE=ACCGTCGCCAAAGAGACAT
PRIMER_RIGHT_73_SEQUENCE=ACCCCTCGGAACTTGTCAAT
PRIMER_LEFT_73=653,19
PRIMER_RIGHT_73=744,20
PRIMER_LEFT_73_TM=60.672
PRIMER_RIGHT_73_TM=60.746
PRIMER_LEFT_73_GC_PERCENT=52.632
PRIMER_RIGHT_73_GC_PERCENT=50.000
PRIMER_LEFT_73_SELF_ANY=3.00
PRIMER_RIGHT_73_SELF_ANY=5.00
PRIMER_LEFT_73_SELF_END=2.00
PRIMER_RIGHT_73_SELF_END=3.00
PRIMER_LEFT_73_END_STABILITY=6.3000
PRIMER_RIGHT_73_END_STABILITY=6.9000
PRIMER_PAIR_73_COMPL_ANY=4.00
PRIMER_PAIR_73_COMPL_END=2.00
PRIMER_PAIR_73_PRODUCT_SIZE=92
PRIMER_PAIR_73_PRODUCT_TM=75.2
PRIMER_PAIR_74_PENALTY=2.424239
PRIMER_LEFT_74_PENALTY=1.233331
PRIMER_RIGHT_74_PENALTY=1.190908
PRIMER_LEFT_74_SEQUENCE=GTGATTCCCTACCGCTGCT
PRIMER_RIGHT_74_SEQUENCE=AAACATCCATCCTCTCCTGGT
PRIMER_LEFT_74=533,19
PRIMER_RIGHT_74=629,21
PRIMER_LEFT_74_TM=60.233
PRIMER_RIGHT_74_TM=59.809
PRIMER_LEFT_74_GC_PERCENT=57.895
PRIMER_RIGHT_74_GC_PERCENT=47.619
PRIMER_LEFT_74_SELF_ANY=3.00
PRIMER_RIGHT_74_SELF_ANY=3.00
PRIMER_LEFT_74_SELF_END=1.00
PRIMER_RIGHT_74_SELF_END=1.00
PRIMER_LEFT_74_END_STABILITY=8.2000
PRIMER_RIGHT_74_END_STABILITY=7.9000
PRIMER_PAIR_74_COMPL_ANY=3.00
PRIMER_PAIR_74_COMPL_END=0.00
PRIMER_PAIR_74_PRODUCT_SIZE=97
PRIMER_PAIR_74_PRODUCT_TM=72.7
PRIMER_PAIR_75_PENALTY=2.456656
PRIMER_LEFT_75_PENALTY=1.271611
PRIMER_RIGHT_75_PENALTY=1.185045
PRIMER_LEFT_75_SEQUENCE=GCCATGTTCTGTGGCAGAC
PRIMER_RIGHT_75_SEQUENCE=TATCAATGCAGGTTTTGGTCC
PRIMER_LEFT_75=302,19
PRIMER_RIGHT_75=392,21
PRIMER_LEFT_75_TM=60.272
PRIMER_RIGHT_75_TM=59.815
PRIMER_LEFT_75_GC_PERCENT=57.895
PRIMER_RIGHT_75_GC_PERCENT=42.857
PRIMER_LEFT_75_SELF_ANY=5.00
PRIMER_RIGHT_75_SELF_ANY=4.00
PRIMER_LEFT_75_SELF_END=3.00
PRIMER_RIGHT_75_SELF_END=3.00
PRIMER_LEFT_75_END_STABILITY=6.4000
PRIMER_RIGHT_75_END_STABILITY=9.1000
PRIMER_PAIR_75_COMPL_ANY=3.00
PRIMER_PAIR_75_COMPL_END=2.00
PRIMER_PAIR_75_PRODUCT_SIZE=91
PRIMER_PAIR_75_PRODUCT_TM=72.7
PRIMER_PAIR_76_PENALTY=2.463300
PRIMER_LEFT_76_PENALTY=1.415114
PRIMER_RIGHT_76_PENALTY=1.048185
PRIMER_LEFT_76_SEQUENCE=CAACCAGTGACCATCCAGAAC
PRIMER_RIGHT_76_SEQUENCE=CTAAGCAGCGGTAGGGAATC
PRIMER_LEFT_76=467,21
PRIMER_RIGHT_76=554,20
PRIMER_LEFT_76_TM=60.415
PRIMER_RIGHT_76_TM=58.952
PRIMER_LEFT_76_GC_PERCENT=52.381
PRIMER_RIGHT_76_GC_PERCENT=55.000
PRIMER_LEFT_76_SELF_ANY=3.00
PRIMER_RIGHT_76_SELF_ANY=3.00
PRIMER_LEFT_76_SELF_END=0.00
PRIMER_RIGHT_76_SELF_END=3.00
PRIMER_LEFT_76_END_STABILITY=6.4000
PRIMER_RIGHT_76_END_STABILITY=6.6000
PRIMER_PAIR_76_COMPL_ANY=3.00
PRIMER_PAIR_76_COMPL_END=0.00
PRIMER_PAIR_76_PRODUCT_SIZE=88
PRIMER_PAIR_76_PRODUCT_TM=76.8
PRIMER_PAIR_77_PENALTY=2.490026
PRIMER_LEFT_77_PENALTY=1.472425
PRIMER_RIGHT_77_PENALTY=1.017601
PRIMER_LEFT_77_SEQUENCE=CCTGCAGTATTGCCAAGAAG
PRIMER_RIGHT_77_SEQUENCE=CAGTTCTGGATGGTCACTGGT
PRIMER_LEFT_77=406,20
PRIMER_RIGHT_77=489,21
PRIMER_LEFT_77_TM=58.528
PRIMER_RIGHT_77_TM=60.018
PRIMER_LEFT_77_GC_PERCENT=50.000
PRIMER_RIGHT_77_GC_PERCENT=52.381
PRIMER_LEFT_77_SELF_ANY=6.00
PRIMER_RIGHT_77_SELF_ANY=4.00
PRIMER_LEFT_77_SELF_END=1.00
PRIMER_RIGHT_77_SELF_END=2.00
PRIMER_LEFT_77_END_STABILITY=6.7000
PRIMER_RIGHT_77_END_STABILITY=7.9000
PRIMER_PAIR_77_COMPL_ANY=4.00
PRIMER_PAIR_77_COMPL_END=1.00
PRIMER_PAIR_77_PRODUCT_SIZE=84
PRIMER_PAIR_77_PRODUCT_TM=73.3
PRIMER_PAIR_78_PENALTY=2.500188
PRIMER_LEFT_78_PENALTY=1.316198
PRIMER_RIGHT_78_PENALTY=1.183989
PRIMER_LEFT_78_SEQUENCE=CCCACTGATGGTAATGCTGG
PRIMER_RIGHT_78_SEQUENCE=CATGTGCATGTTCAGTCTGC
PRIMER_LEFT_78=260,20
PRIMER_RIGHT_78=334,20
PRIMER_LEFT_78_TM=61.316
PRIMER_RIGHT_78_TM=58.816
PRIMER_LEFT_78_GC_PERCENT=55.000
PRIMER_RIGHT_78_GC_PERCENT=50.000
PRIMER_LEFT_78_SELF_ANY=3.00
PRIMER_RIGHT_78_SELF_ANY=6.00
PRIMER_LEFT_78_SELF_END=0.00
PRIMER_RIGHT_78_SELF_END=3.00
PRIMER_LEFT_78_END_STABILITY=9.7000
PRIMER_RIGHT_78_END_STABILITY=8.2000
PRIMER_PAIR_78_COMPL_ANY=5.00
PRIMER_PAIR_78_COMPL_END=1.00
PRIMER_PAIR_78_PRODUCT_SIZE=75
PRIMER_PAIR_78_PRODUCT_TM=74.3
PRIMER_PAIR_79_PENALTY=2.528184
PRIMER_LEFT_79_PENALTY=1.292120
PRIMER_RIGHT_79_PENALTY=1.236064
PRIMER_LEFT_79_SEQUENCE=GCCATGTTCTGTGGCAGACT
PRIMER_RIGHT_79_SEQUENCE=AGGTTTTGGTCCCTGATGG
PRIMER_LEFT_79=302,20
PRIMER_RIGHT_79=383,19
PRIMER_LEFT_79_TM=61.292
PRIMER_RIGHT_79_TM=59.764
PRIMER_LEFT_79_GC_PERCENT=55.000
PRIMER_RIGHT_79_GC_PERCENT=52.632
PRIMER_LEFT_79_SELF_ANY=5.00
PRIMER_RIGHT_79_SELF_ANY=3.00
PRIMER_LEFT_79_SELF_END=3.00
PRIMER_RIGHT_79_SELF_END=3.00
PRIMER_LEFT_79_END_STABILITY=6.1000
PRIMER_RIGHT_79_END_STABILITY=8.1000
PRIMER_PAIR_79_COMPL_ANY=4.00
PRIMER_PAIR_79_COMPL_END=0.00
PRIMER_PAIR_79_PRODUCT_SIZE=82
PRIMER_PAIR_79_PRODUCT_TM=72.6
PRIMER_PAIR_80_PENALTY=2.535486
PRIMER_LEFT_80_PENALTY=0.704204
PRIMER_RIGHT_80_PENALTY=1.831282
PRIMER_LEFT_80_SEQUENCE=CATGAATGTCCAGAATGGGA
PRIMER_RIGHT_80_SEQUENCE=CAGGATGCCTTCCTTGGTATC
PRIMER_LEFT_80=331,20
PRIMER_RIGHT_80=409,21
PRIMER_LEFT_80_TM=59.296
PRIMER_RIGHT_80_TM=60.831
PRIMER_LEFT_80_GC_PERCENT=45.000
PRIMER_RIGHT_80_GC_PERCENT=52.381
PRIMER_LEFT_80_SELF_ANY=4.00
PRIMER_RIGHT_80_SELF_ANY=7.00
PRIMER_LEFT_80_SELF_END=1.00
PRIMER_RIGHT_80_SELF_END=2.00
PRIMER_LEFT_80_END_STABILITY=9.7000
PRIMER_RIGHT_80_END_STABILITY=5.3000
PRIMER_PAIR_80_COMPL_ANY=3.00
PRIMER_PAIR_80_COMPL_END=2.00
PRIMER_PAIR_80_PRODUCT_SIZE=79
PRIMER_PAIR_80_PRODUCT_TM=72.0
PRIMER_PAIR_81_PENALTY=2.562163
PRIMER_LEFT_81_PENALTY=1.185045
PRIMER_RIGHT_81_PENALTY=1.377118
PRIMER_LEFT_81_SEQUENCE=GGACCAAAACCTGCATTGATA
PRIMER_RIGHT_81_SEQUENCE=GTTGGTTGGCTTCTACCACA
PRIMER_LEFT_81=372,21
PRIMER_RIGHT_81=470,20
PRIMER_LEFT_81_TM=59.815
PRIMER_RIGHT_81_TM=58.623
PRIMER_LEFT_81_GC_PERCENT=42.857
PRIMER_RIGHT_81_GC_PERCENT=50.000
PRIMER_LEFT_81_SELF_ANY=4.00
PRIMER_RIGHT_81_SELF_ANY=4.00
PRIMER_LEFT_81_SELF_END=2.00
PRIMER_RIGHT_81_SELF_END=2.00
PRIMER_LEFT_81_END_STABILITY=5.9000
PRIMER_RIGHT_81_END_STABILITY=8.2000
PRIMER_PAIR_81_COMPL_ANY=5.00
PRIMER_PAIR_81_COMPL_END=2.00
PRIMER_PAIR_81_PRODUCT_SIZE=99
PRIMER_PAIR_81_PRODUCT_TM=73.7
PRIMER_PAIR_82_PENALTY=2.575832
PRIMER_LEFT_82_PENALTY=1.558232
PRIMER_RIGHT_82_PENALTY=1.017601
PRIMER_LEFT_82_SEQUENCE=TGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_82_SEQUENCE=ACCAGTTCTGGATGGTCACTG
PRIMER_LEFT_82=416,20
PRIMER_RIGHT_82=491,21
PRIMER_LEFT_82_TM=58.442
PRIMER_RIGHT_82_TM=60.018
PRIMER_LEFT_82_GC_PERCENT=50.000
PRIMER_RIGHT_82_GC_PERCENT=52.381
PRIMER_LEFT_82_SELF_ANY=4.00
PRIMER_RIGHT_82_SELF_ANY=6.00
PRIMER_LEFT_82_SELF_END=1.00
PRIMER_RIGHT_82_SELF_END=3.00
PRIMER_LEFT_82_END_STABILITY=8.2000
PRIMER_RIGHT_82_END_STABILITY=6.7000
PRIMER_PAIR_82_COMPL_ANY=4.00
PRIMER_PAIR_82_COMPL_END=2.00
PRIMER_PAIR_82_PRODUCT_SIZE=76
PRIMER_PAIR_82_PRODUCT_TM=72.5
PRIMER_PAIR_83_PENALTY=2.583424
PRIMER_LEFT_83_PENALTY=1.429867
PRIMER_RIGHT_83_PENALTY=1.153556
PRIMER_LEFT_83_SEQUENCE=TCACCAATGTGGTAGAAGCC
PRIMER_RIGHT_83_SEQUENCE=ATGGGTCTTGCACTGCTTG
PRIMER_LEFT_83=444,20
PRIMER_RIGHT_83=523,19
PRIMER_LEFT_83_TM=58.570
PRIMER_RIGHT_83_TM=59.846
PRIMER_LEFT_83_GC_PERCENT=50.000
PRIMER_RIGHT_83_GC_PERCENT=52.632
PRIMER_LEFT_83_SELF_ANY=6.00
PRIMER_RIGHT_83_SELF_ANY=5.00
PRIMER_LEFT_83_SELF_END=1.00
PRIMER_RIGHT_83_SELF_END=3.00
PRIMER_LEFT_83_END_STABILITY=9.7000
PRIMER_RIGHT_83_END_STABILITY=8.5000
PRIMER_PAIR_83_COMPL_ANY=4.00
PRIMER_PAIR_83_COMPL_END=3.00
PRIMER_PAIR_83_PRODUCT_SIZE=80
PRIMER_PAIR_83_PRODUCT_TM=75.5
PRIMER_PAIR_84_PENALTY=2.613176
PRIMER_LEFT_84_PENALTY=1.174455
PRIMER_RIGHT_84_PENALTY=1.438721
PRIMER_LEFT_84_SEQUENCE=ACTCATCTTCACTGGCACACC
PRIMER_RIGHT_84_SEQUENCE=GCAGCAACATGCCGTAGTC
PRIMER_LEFT_84=635,21
PRIMER_RIGHT_84=716,19
PRIMER_LEFT_84_TM=60.174
PRIMER_RIGHT_84_TM=60.439
PRIMER_LEFT_84_GC_PERCENT=52.381
PRIMER_RIGHT_84_GC_PERCENT=57.895
PRIMER_LEFT_84_SELF_ANY=3.00
PRIMER_RIGHT_84_SELF_ANY=4.00
PRIMER_LEFT_84_SELF_END=0.00
PRIMER_RIGHT_84_SELF_END=1.00
PRIMER_LEFT_84_END_STABILITY=7.6000
PRIMER_RIGHT_84_END_STABILITY=5.4000
PRIMER_PAIR_84_COMPL_ANY=4.00
PRIMER_PAIR_84_COMPL_END=0.00
PRIMER_PAIR_84_PRODUCT_SIZE=82
PRIMER_PAIR_84_PRODUCT_TM=73.6
PRIMER_PAIR_85_PENALTY=2.613182
PRIMER_LEFT_85_PENALTY=1.377118
PRIMER_RIGHT_85_PENALTY=1.236064
PRIMER_LEFT_85_SEQUENCE=TGTGGTAGAAGCCAACCAAC
PRIMER_RIGHT_85_SEQUENCE=AAAGTGGGGATGGGTCTTG
PRIMER_LEFT_85=451,20
PRIMER_RIGHT_85=532,19
PRIMER_LEFT_85_TM=58.623
PRIMER_RIGHT_85_TM=59.764
PRIMER_LEFT_85_GC_PERCENT=50.000
PRIMER_RIGHT_85_GC_PERCENT=52.632
PRIMER_LEFT_85_SELF_ANY=4.00
PRIMER_RIGHT_85_SELF_ANY=3.00
PRIMER_LEFT_85_SELF_END=2.00
PRIMER_RIGHT_85_SELF_END=0.00
PRIMER_LEFT_85_END_STABILITY=8.2000
PRIMER_RIGHT_85_END_STABILITY=7.0000
PRIMER_PAIR_85_COMPL_ANY=4.00
PRIMER_PAIR_85_COMPL_END=2.00
PRIMER_PAIR_85_PRODUCT_SIZE=82
PRIMER_PAIR_85_PRODUCT_TM=76.1
PRIMER_PAIR_86_PENALTY=2.625605
PRIMER_LEFT_86_PENALTY=1.608005
PRIMER_RIGHT_86_PENALTY=1.017601
PRIMER_LEFT_86_SEQUENCE=CCTGCAGTATTGCCAAGAAGT
PRIMER_RIGHT_86_SEQUENCE=CACCAGTTCTGGATGGTCACT
PRIMER_LEFT_86=406,21
PRIMER_RIGHT_86=492,21
PRIMER_LEFT_86_TM=59.392
PRIMER_RIGHT_86_TM=60.018
PRIMER_LEFT_86_GC_PERCENT=47.619
PRIMER_RIGHT_86_GC_PERCENT=52.381
PRIMER_LEFT_86_SELF_ANY=6.00
PRIMER_RIGHT_86_SELF_ANY=6.00
PRIMER_LEFT_86_SELF_END=1.00
PRIMER_RIGHT_86_SELF_END=1.00
PRIMER_LEFT_86_END_STABILITY=6.4000
PRIMER_RIGHT_86_END_STABILITY=6.4000
PRIMER_PAIR_86_COMPL_ANY=4.00
PRIMER_PAIR_86_COMPL_END=3.00
PRIMER_PAIR_86_PRODUCT_SIZE=87
PRIMER_PAIR_86_PRODUCT_TM=73.7
PRIMER_PAIR_87_PENALTY=2.642454
PRIMER_LEFT_87_PENALTY=1.409123
PRIMER_RIGHT_87_PENALTY=1.233331
PRIMER_LEFT_87_SEQUENCE=GGTAGAAGCCAACCAACCAGT
PRIMER_RIGHT_87_SEQUENCE=AGCAGCGGTAGGGAATCAC
PRIMER_LEFT_87=454,21
PRIMER_RIGHT_87=551,19
PRIMER_LEFT_87_TM=60.409
PRIMER_RIGHT_87_TM=60.233
PRIMER_LEFT_87_GC_PERCENT=52.381
PRIMER_RIGHT_87_GC_PERCENT=57.895
PRIMER_LEFT_87_SELF_ANY=3.00
PRIMER_RIGHT_87_SELF_ANY=3.00
PRIMER_LEFT_87_SELF_END=2.00
PRIMER_RIGHT_87_SELF_END=3.00
PRIMER_LEFT_87_END_STABILITY=7.9000
PRIMER_RIGHT_87_END_STABILITY=6.3000
PRIMER_PAIR_87_COMPL_ANY=4.00
PRIMER_PAIR_87_COMPL_END=2.00
PRIMER_PAIR_87_PRODUCT_SIZE=98
PRIMER_PAIR_87_PRODUCT_TM=77.6
PRIMER_PAIR_88_PENALTY=2.645233
PRIMER_LEFT_88_PENALTY=1.239865
PRIMER_RIGHT_88_PENALTY=1.405369
PRIMER_LEFT_88_SEQUENCE=GATTCCCTACCGCTGCTTAGT
PRIMER_RIGHT_88_SEQUENCE=TTCGCAAACATCCATCCTC
PRIMER_LEFT_88=535,21
PRIMER_RIGHT_88=634,19
PRIMER_LEFT_88_TM=59.760
PRIMER_RIGHT_88_TM=59.595
PRIMER_LEFT_88_GC_PERCENT=52.381
PRIMER_RIGHT_88_GC_PERCENT=47.368
PRIMER_LEFT_88_SELF_ANY=3.00
PRIMER_RIGHT_88_SELF_ANY=4.00
PRIMER_LEFT_88_SELF_END=1.00
PRIMER_RIGHT_88_SELF_END=0.00
PRIMER_LEFT_88_END_STABILITY=5.7000
PRIMER_RIGHT_88_END_STABILITY=7.9000
PRIMER_PAIR_88_COMPL_ANY=3.00
PRIMER_PAIR_88_COMPL_END=0.00
PRIMER_PAIR_88_PRODUCT_SIZE=100
PRIMER_PAIR_88_PRODUCT_TM=73.2
PRIMER_PAIR_89_PENALTY=2.697526
PRIMER_LEFT_89_PENALTY=1.461462
PRIMER_RIGHT_89_PENALTY=1.236064
PRIMER_LEFT_89_SEQUENCE=TGGTAGAAGCCAACCAACCA
PRIMER_RIGHT_89_SEQUENCE=CAAAGTGGGGATGGGTCTT
PRIMER_LEFT_89=453,20
PRIMER_RIGHT_89=533,19
PRIMER_LEFT_89_TM=61.461
PRIMER_RIGHT_89_TM=59.764
PRIMER_LEFT_89_GC_PERCENT=50.000
PRIMER_RIGHT_89_GC_PERCENT=52.632
PRIMER_LEFT_89_SELF_ANY=4.00
PRIMER_RIGHT_89_SELF_ANY=3.00
PRIMER_LEFT_89_SELF_END=1.00
PRIMER_RIGHT_89_SELF_END=0.00
PRIMER_LEFT_89_END_STABILITY=8.2000
PRIMER_RIGHT_89_END_STABILITY=6.4000
PRIMER_PAIR_89_COMPL_ANY=4.00
PRIMER_PAIR_89_COMPL_END=3.00
PRIMER_PAIR_89_PRODUCT_SIZE=81
PRIMER_PAIR_89_PRODUCT_TM=76.3
PRIMER_PAIR_90_PENALTY=2.715470
PRIMER_LEFT_90_PENALTY=1.043090
PRIMER_RIGHT_90_PENALTY=1.672380
PRIMER_LEFT_90_SEQUENCE=CTTCTCGTTCCTGACAAGTGC
PRIMER_RIGHT_90_SEQUENCE=ATGTCTCTTTGGCGACGGT
PRIMER_LEFT_90=578,21
PRIMER_RIGHT_90=671,19
PRIMER_LEFT_90_TM=60.043
PRIMER_RIGHT_90_TM=60.672
PRIMER_LEFT_90_GC_PERCENT=52.381
PRIMER_RIGHT_90_GC_PERCENT=52.632
PRIMER_LEFT_90_SELF_ANY=4.00
PRIMER_RIGHT_90_SELF_ANY=3.00
PRIMER_LEFT_90_SELF_END=2.00
PRIMER_RIGHT_90_SELF_END=3.00
PRIMER_LEFT_90_END_STABILITY=7.9000
PRIMER_RIGHT_90_END_STABILITY=9.3000
PRIMER_PAIR_90_COMPL_ANY=4.00
PRIMER_PAIR_90_COMPL_END=1.00
PRIMER_PAIR_90_PRODUCT_SIZE=94
PRIMER_PAIR_90_PRODUCT_TM=73.1
PRIMER_PAIR_91_PENALTY=2.751265
PRIMER_LEFT_91_PENALTY=1.515201
PRIMER_RIGHT_91_PENALTY=1.236064
PRIMER_LEFT_91_SEQUENCE=AATGTGGTAGAAGCCAACCAA
PRIMER_RIGHT_91_SEQUENCE=AGGGAATCACAAAGTGGGG
PRIMER_LEFT_91=449,21
PRIMER_RIGHT_91=542,19
PRIMER_LEFT_91_TM=59.485
PRIMER_RIGHT_91_TM=59.764
PRIMER_LEFT_91_GC_PERCENT=42.857
PRIMER_RIGHT_91_GC_PERCENT=52.632
PRIMER_LEFT_91_SELF_ANY=4.00
PRIMER_RIGHT_91_SELF_ANY=3.00
PRIMER_LEFT_91_SELF_END=2.00
PRIMER_RIGHT_91_SELF_END=0.00
PRIMER_LEFT_91_END_STABILITY=8.2000
PRIMER_RIGHT_91_END_STABILITY=11.2000
PRIMER_PAIR_91_COMPL_ANY=4.00
PRIMER_PAIR_91_COMPL_END=1.00
PRIMER_PAIR_91_PRODUCT_SIZE=94
PRIMER_PAIR_91_PRODUCT_TM=76.2
PRIMER_PAIR_92_PENALTY=2.755288
PRIMER_LEFT_92_PENALTY=1.159466
PRIMER_RIGHT_92_PENALTY=1.595822
PRIMER_LEFT_92_SEQUENCE=GAGAGGATGGATGTTTGCGA
PRIMER_RIGHT_92_SEQUENCE=ACATGCCGTAGTCATGCAAGT
PRIMER_LEFT_92=614,20
PRIMER_RIGHT_92=710,21
PRIMER_LEFT_92_TM=61.159
PRIMER_RIGHT_92_TM=60.596
PRIMER_LEFT_92_GC_PERCENT=50.000
PRIMER_RIGHT_92_GC_PERCENT=47.619
PRIMER_LEFT_92_SELF_ANY=2.00
PRIMER_RIGHT_92_SELF_ANY=6.00
PRIMER_LEFT_92_SELF_END=2.00
PRIMER_RIGHT_92_SELF_END=3.00
PRIMER_LEFT_92_END_STABILITY=10.2000
PRIMER_RIGHT_92_END_STABILITY=6.7000
PRIMER_PAIR_92_COMPL_ANY=4.00
PRIMER_PAIR_92_COMPL_END=1.00
PRIMER_PAIR_92_PRODUCT_SIZE=97
PRIMER_PAIR_92_PRODUCT_TM=74.0
PRIMER_PAIR_93_PENALTY=2.766605
PRIMER_LEFT_93_PENALTY=1.526740
PRIMER_RIGHT_93_PENALTY=1.239865
PRIMER_LEFT_93_SEQUENCE=ACCATCCAGAACTGGTGCAA
PRIMER_RIGHT_93_SEQUENCE=ACTAAGCAGCGGTAGGGAATC
PRIMER_LEFT_93=476,20
PRIMER_RIGHT_93=555,21
PRIMER_LEFT_93_TM=61.527
PRIMER_RIGHT_93_TM=59.760
PRIMER_LEFT_93_GC_PERCENT=50.000
PRIMER_RIGHT_93_GC_PERCENT=52.381
PRIMER_LEFT_93_SELF_ANY=6.00
PRIMER_RIGHT_93_SELF_ANY=3.00
PRIMER_LEFT_93_SELF_END=2.00
PRIMER_RIGHT_93_SELF_END=3.00
PRIMER_LEFT_93_END_STABILITY=8.8000
PRIMER_RIGHT_93_END_STABILITY=6.6000
PRIMER_PAIR_93_COMPL_ANY=4.00
PRIMER_PAIR_93_COMPL_END=0.00
PRIMER_PAIR_93_PRODUCT_SIZE=80
PRIMER_PAIR_93_PRODUCT_TM=76.0
PRIMER_PAIR_94_PENALTY=2.772752
PRIMER_LEFT_94_PENALTY=2.083070
PRIMER_RIGHT_94_PENALTY=0.689681
PRIMER_LEFT_94_SEQUENCE=CATTGATACCAAGGAAGGCAT
PRIMER_RIGHT_94_SEQUENCE=TCACTGGTTGGTTGGCTTCT
PRIMER_LEFT_94=385,21
PRIMER_RIGHT_94=476,20
PRIMER_LEFT_94_TM=58.917
PRIMER_RIGHT_94_TM=60.690
PRIMER_LEFT_94_GC_PERCENT=42.857
PRIMER_RIGHT_94_GC_PERCENT=50.000
PRIMER_LEFT_94_SELF_ANY=6.00
PRIMER_RIGHT_94_SELF_ANY=3.00
PRIMER_LEFT_94_SELF_END=2.00
PRIMER_RIGHT_94_SELF_END=0.00
PRIMER_LEFT_94_END_STABILITY=9.6000
PRIMER_RIGHT_94_END_STABILITY=6.7000
PRIMER_PAIR_94_COMPL_ANY=5.00
PRIMER_PAIR_94_COMPL_END=3.00
PRIMER_PAIR_94_PRODUCT_SIZE=92
PRIMER_PAIR_94_PRODUCT_TM=73.0
PRIMER_PAIR_95_PENALTY=2.793905
PRIMER_LEFT_95_PENALTY=0.663548
PRIMER_RIGHT_95_PENALTY=2.130358
PRIMER_LEFT_95_SEQUENCE=AATGGGAAGTGGGATTCAGA
PRIMER_RIGHT_95_SEQUENCE=TCAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_95=344,20
PRIMER_RIGHT_95=435,22
PRIMER_LEFT_95_TM=59.336
PRIMER_RIGHT_95_TM=60.130
PRIMER_LEFT_95_GC_PERCENT=45.000
PRIMER_RIGHT_95_GC_PERCENT=45.455
PRIMER_LEFT_95_SELF_ANY=3.00
PRIMER_RIGHT_95_SELF_ANY=5.00
PRIMER_LEFT_95_SELF_END=3.00
PRIMER_RIGHT_95_SELF_END=3.00
PRIMER_LEFT_95_END_STABILITY=6.7000
PRIMER_RIGHT_95_END_STABILITY=8.4000
PRIMER_PAIR_95_COMPL_ANY=5.00
PRIMER_PAIR_95_COMPL_END=1.00
PRIMER_PAIR_95_PRODUCT_SIZE=92
PRIMER_PAIR_95_PRODUCT_TM=73.0
PRIMER_PAIR_96_PENALTY=2.802419
PRIMER_LEFT_96_PENALTY=1.236064
PRIMER_RIGHT_96_PENALTY=1.566355
PRIMER_LEFT_96_SEQUENCE=CAAGACCCATCCCCACTTT
PRIMER_RIGHT_96_SEQUENCE=GGAACGAGAAGGGCATCACT
PRIMER_LEFT_96=514,19
PRIMER_RIGHT_96=588,20
PRIMER_LEFT_96_TM=59.764
PRIMER_RIGHT_96_TM=61.566
PRIMER_LEFT_96_GC_PERCENT=52.632
PRIMER_RIGHT_96_GC_PERCENT=55.000
PRIMER_LEFT_96_SELF_ANY=3.00
PRIMER_RIGHT_96_SELF_ANY=2.00
PRIMER_LEFT_96_SELF_END=0.00
PRIMER_RIGHT_96_SELF_END=2.00
PRIMER_LEFT_96_END_STABILITY=6.7000
PRIMER_RIGHT_96_END_STABILITY=6.4000
PRIMER_PAIR_96_COMPL_ANY=4.00
PRIMER_PAIR_96_COMPL_END=0.00
PRIMER_PAIR_96_PRODUCT_SIZE=75
PRIMER_PAIR_96_PRODUCT_TM=72.7
PRIMER_PAIR_97_PENALTY=2.803473
PRIMER_LEFT_97_PENALTY=1.059537
PRIMER_RIGHT_97_PENALTY=1.743936
PRIMER_LEFT_97_SEQUENCE=CCAGGAGAGGATGGATGTTT
PRIMER_RIGHT_97_SEQUENCE=ATGCCGTAGTCATGCAAGTT
PRIMER_LEFT_97=610,20
PRIMER_RIGHT_97=708,20
PRIMER_LEFT_97_TM=58.940
PRIMER_RIGHT_97_TM=58.256
PRIMER_LEFT_97_GC_PERCENT=50.000
PRIMER_RIGHT_97_GC_PERCENT=45.000
PRIMER_LEFT_97_SELF_ANY=3.00
PRIMER_RIGHT_97_SELF_ANY=4.00
PRIMER_LEFT_97_SELF_END=0.00
PRIMER_RIGHT_97_SELF_END=3.00
PRIMER_LEFT_97_END_STABILITY=7.0000
PRIMER_RIGHT_97_END_STABILITY=6.7000
PRIMER_PAIR_97_COMPL_ANY=4.00
PRIMER_PAIR_97_COMPL_END=1.00
PRIMER_PAIR_97_PRODUCT_SIZE=99
PRIMER_PAIR_97_PRODUCT_TM=74.5
PRIMER_PAIR_98_PENALTY=2.807694
PRIMER_LEFT_98_PENALTY=0.807658
PRIMER_RIGHT_98_PENALTY=2.000037
PRIMER_LEFT_98_SEQUENCE=GATCCATCAGGGACCAAAAC
PRIMER_RIGHT_98_SEQUENCE=TTCTACCACATTGGTGATCTGC
PRIMER_LEFT_98=362,20
PRIMER_RIGHT_98=460,22
PRIMER_LEFT_98_TM=59.192
PRIMER_RIGHT_98_TM=60.000
PRIMER_LEFT_98_GC_PERCENT=50.000
PRIMER_RIGHT_98_GC_PERCENT=45.455
PRIMER_LEFT_98_SELF_ANY=5.00
PRIMER_RIGHT_98_SELF_ANY=6.00
PRIMER_LEFT_98_SELF_END=0.00
PRIMER_RIGHT_98_SELF_END=2.00
PRIMER_LEFT_98_END_STABILITY=7.0000
PRIMER_RIGHT_98_END_STABILITY=8.2000
PRIMER_PAIR_98_COMPL_ANY=5.00
PRIMER_PAIR_98_COMPL_END=1.00
PRIMER_PAIR_98_PRODUCT_SIZE=99
PRIMER_PAIR_98_PRODUCT_TM=73.3
PRIMER_PAIR_99_PENALTY=2.808409
PRIMER_LEFT_99_PENALTY=1.251196
PRIMER_RIGHT_99_PENALTY=1.557214
PRIMER_LEFT_99_SEQUENCE=TGTAAGTGATGCCCTTCTCGT
PRIMER_RIGHT_99_SEQUENCE=CCAGTGAAGATGAGTTTCGC
PRIMER_LEFT_99=565,21
PRIMER_RIGHT_99=649,20
PRIMER_LEFT_99_TM=59.749
PRIMER_RIGHT_99_TM=58.443
PRIMER_LEFT_99_GC_PERCENT=47.619
PRIMER_RIGHT_99_GC_PERCENT=50.000
PRIMER_LEFT_99_SELF_ANY=4.00
PRIMER_RIGHT_99_SELF_ANY=3.00
PRIMER_LEFT_99_SELF_END=2.00
PRIMER_RIGHT_99_SELF_END=3.00
PRIMER_LEFT_99_END_STABILITY=8.1000
PRIMER_RIGHT_99_END_STABILITY=10.2000
PRIMER_PAIR_99_COMPL_ANY=4.00
PRIMER_PAIR_99_COMPL_END=1.00
PRIMER_PAIR_99_PRODUCT_SIZE=85
PRIMER_PAIR_99_PRODUCT_TM=71.7
PRIMER_PAIR_100_PENALTY=2.812889
PRIMER_LEFT_100_PENALTY=1.255675
PRIMER_RIGHT_100_PENALTY=1.557214
PRIMER_LEFT_100_SEQUENCE=TTGTAAGTGATGCCCTTCTCG
PRIMER_RIGHT_100_SEQUENCE=GCCAGTGAAGATGAGTTTCG
PRIMER_LEFT_100=564,21
PRIMER_RIGHT_100=650,20
PRIMER_LEFT_100_TM=60.256
PRIMER_RIGHT_100_TM=58.443
PRIMER_LEFT_100_GC_PERCENT=47.619
PRIMER_RIGHT_100_GC_PERCENT=50.000
PRIMER_LEFT_100_SELF_ANY=4.00
PRIMER_RIGHT_100_SELF_ANY=3.00
PRIMER_LEFT_100_SELF_END=2.00
PRIMER_RIGHT_100_SELF_END=2.00
PRIMER_LEFT_100_END_STABILITY=8.4000
PRIMER_RIGHT_100_END_STABILITY=9.0000
PRIMER_PAIR_100_COMPL_ANY=4.00
PRIMER_PAIR_100_COMPL_END=2.00
PRIMER_PAIR_100_PRODUCT_SIZE=87
PRIMER_PAIR_100_PRODUCT_TM=71.9
PRIMER_PAIR_101_PENALTY=2.835338
PRIMER_LEFT_101_PENALTY=1.557214
PRIMER_RIGHT_101_PENALTY=1.278124
PRIMER_LEFT_101_SEQUENCE=ACCCACTGATGGTAATGCTG
PRIMER_RIGHT_101_SEQUENCE=ATCCCACTTCCCATTCTGG
PRIMER_LEFT_101=259,20
PRIMER_RIGHT_101=358,19
PRIMER_LEFT_101_TM=58.443
PRIMER_RIGHT_101_TM=59.722
PRIMER_LEFT_101_GC_PERCENT=50.000
PRIMER_RIGHT_101_GC_PERCENT=52.632
PRIMER_LEFT_101_SELF_ANY=3.00
PRIMER_RIGHT_101_SELF_ANY=3.00
PRIMER_LEFT_101_SELF_END=1.00
PRIMER_RIGHT_101_SELF_END=3.00
PRIMER_LEFT_101_END_STABILITY=8.5000
PRIMER_RIGHT_101_END_STABILITY=8.2000
PRIMER_PAIR_101_COMPL_ANY=5.00
PRIMER_PAIR_101_COMPL_END=3.00
PRIMER_PAIR_101_PRODUCT_SIZE=100
PRIMER_PAIR_101_PRODUCT_TM=75.2
PRIMER_PAIR_102_PENALTY=2.840973
PRIMER_LEFT_102_PENALTY=1.977994
PRIMER_RIGHT_102_PENALTY=0.862979
PRIMER_LEFT_102_SEQUENCE=CCAAAGAGACATGCAGTGAGA
PRIMER_RIGHT_102_SEQUENCE=TCTACCCCTCGGAACTTGTC
PRIMER_LEFT_102=660,21
PRIMER_RIGHT_102=747,20
PRIMER_LEFT_102_TM=59.022
PRIMER_RIGHT_102_TM=59.137
PRIMER_LEFT_102_GC_PERCENT=47.619
PRIMER_RIGHT_102_GC_PERCENT=55.000
PRIMER_LEFT_102_SELF_ANY=4.00
PRIMER_RIGHT_102_SELF_ANY=3.00
PRIMER_LEFT_102_SELF_END=0.00
PRIMER_RIGHT_102_SELF_END=1.00
PRIMER_LEFT_102_END_STABILITY=6.7000
PRIMER_RIGHT_102_END_STABILITY=6.7000
PRIMER_PAIR_102_COMPL_ANY=4.00
PRIMER_PAIR_102_COMPL_END=2.00
PRIMER_PAIR_102_PRODUCT_SIZE=88
PRIMER_PAIR_102_PRODUCT_TM=74.1
PRIMER_PAIR_103_PENALTY=2.919658
PRIMER_LEFT_103_PENALTY=1.236064
PRIMER_RIGHT_103_PENALTY=1.683594
PRIMER_LEFT_103_SEQUENCE=CCCCACTTTGTGATTCCCT
PRIMER_RIGHT_103_SEQUENCE=ATTTGCACTTGTCAGGAACGA
PRIMER_LEFT_103=524,19
PRIMER_RIGHT_103=602,21
PRIMER_LEFT_103_TM=59.764
PRIMER_RIGHT_103_TM=60.684
PRIMER_LEFT_103_GC_PERCENT=52.632
PRIMER_RIGHT_103_GC_PERCENT=42.857
PRIMER_LEFT_103_SELF_ANY=3.00
PRIMER_RIGHT_103_SELF_ANY=4.00
PRIMER_LEFT_103_SELF_END=1.00
PRIMER_RIGHT_103_SELF_END=1.00
PRIMER_LEFT_103_END_STABILITY=9.4000
PRIMER_RIGHT_103_END_STABILITY=8.4000
PRIMER_PAIR_103_COMPL_ANY=4.00
PRIMER_PAIR_103_COMPL_END=2.00
PRIMER_PAIR_103_PRODUCT_SIZE=79
PRIMER_PAIR_103_PRODUCT_TM=72.0
PRIMER_PAIR_104_PENALTY=2.929327
PRIMER_LEFT_104_PENALTY=1.991012
PRIMER_RIGHT_104_PENALTY=0.938316
PRIMER_LEFT_104_SEQUENCE=GCCAAAGAGACATGCAGTGAG
PRIMER_RIGHT_104_SEQUENCE=ACACAAACTCTACCCCTCGG
PRIMER_LEFT_104=659,21
PRIMER_RIGHT_104=755,20
PRIMER_LEFT_104_TM=60.991
PRIMER_RIGHT_104_TM=59.062
PRIMER_LEFT_104_GC_PERCENT=52.381
PRIMER_RIGHT_104_GC_PERCENT=55.000
PRIMER_LEFT_104_SELF_ANY=4.00
PRIMER_RIGHT_104_SELF_ANY=2.00
PRIMER_LEFT_104_SELF_END=0.00
PRIMER_RIGHT_104_SELF_END=2.00
PRIMER_LEFT_104_END_STABILITY=6.4000
PRIMER_RIGHT_104_END_STABILITY=9.9000
PRIMER_PAIR_104_COMPL_ANY=4.00
PRIMER_PAIR_104_COMPL_END=1.00
PRIMER_PAIR_104_PRODUCT_SIZE=97
PRIMER_PAIR_104_PRODUCT_TM=74.4
PRIMER_PAIR_105_PENALTY=2.936620
PRIMER_LEFT_105_PENALTY=1.810378
PRIMER_RIGHT_105_PENALTY=1.126243
PRIMER_LEFT_105_SEQUENCE=CAAGCAGTGCAAGACCCATC
PRIMER_RIGHT_105_SEQUENCE=CGAGAAGGGCATCACTTACA
PRIMER_LEFT_105=505,20
PRIMER_RIGHT_105=584,20
PRIMER_LEFT_105_TM=61.810
PRIMER_RIGHT_105_TM=58.874
PRIMER_LEFT_105_GC_PERCENT=55.000
PRIMER_RIGHT_105_GC_PERCENT=50.000
PRIMER_LEFT_105_SELF_ANY=5.00
PRIMER_RIGHT_105_SELF_ANY=4.00
PRIMER_LEFT_105_SELF_END=0.00
PRIMER_RIGHT_105_SELF_END=0.00
PRIMER_LEFT_105_END_STABILITY=8.1000
PRIMER_RIGHT_105_END_STABILITY=6.0000
PRIMER_PAIR_105_COMPL_ANY=5.00
PRIMER_PAIR_105_COMPL_END=2.00
PRIMER_PAIR_105_PRODUCT_SIZE=80
PRIMER_PAIR_105_PRODUCT_TM=73.4
PRIMER_PAIR_106_PENALTY=2.957123
PRIMER_LEFT_106_PENALTY=1.342473
PRIMER_RIGHT_106_PENALTY=1.614650
PRIMER_LEFT_106_SEQUENCE=TTGTGATTCCCTACCGCTG
PRIMER_RIGHT_106_SEQUENCE=AACATCCATCCTCTCCTGGT
PRIMER_LEFT_106=531,19
PRIMER_RIGHT_106=628,20
PRIMER_LEFT_106_TM=59.658
PRIMER_RIGHT_106_TM=58.385
PRIMER_LEFT_106_GC_PERCENT=52.632
PRIMER_RIGHT_106_GC_PERCENT=50.000
PRIMER_LEFT_106_SELF_ANY=3.00
PRIMER_RIGHT_106_SELF_ANY=3.00
PRIMER_LEFT_106_SELF_END=2.00
PRIMER_RIGHT_106_SELF_END=1.00
PRIMER_LEFT_106_END_STABILITY=10.2000
PRIMER_RIGHT_106_END_STABILITY=7.9000
PRIMER_PAIR_106_COMPL_ANY=3.00
PRIMER_PAIR_106_COMPL_END=1.00
PRIMER_PAIR_106_PRODUCT_SIZE=98
PRIMER_PAIR_106_PRODUCT_TM=72.6
PRIMER_PAIR_107_PENALTY=2.960724
PRIMER_LEFT_107_PENALTY=1.532916
PRIMER_RIGHT_107_PENALTY=1.427808
PRIMER_LEFT_107_SEQUENCE=CATGCACATGAATGTCCAGAA
PRIMER_RIGHT_107_SEQUENCE=TTCCTTGGTATCAATGCAGG
PRIMER_LEFT_107=325,21
PRIMER_RIGHT_107=400,20
PRIMER_LEFT_107_TM=60.533
PRIMER_RIGHT_107_TM=58.572
PRIMER_LEFT_107_GC_PERCENT=42.857
PRIMER_RIGHT_107_GC_PERCENT=45.000
PRIMER_LEFT_107_SELF_ANY=8.00
PRIMER_RIGHT_107_SELF_ANY=6.00
PRIMER_LEFT_107_SELF_END=3.00
PRIMER_RIGHT_107_SELF_END=2.00
PRIMER_LEFT_107_END_STABILITY=7.0000
PRIMER_RIGHT_107_END_STABILITY=9.7000
PRIMER_PAIR_107_COMPL_ANY=4.00
PRIMER_PAIR_107_COMPL_END=3.00
PRIMER_PAIR_107_PRODUCT_SIZE=76
PRIMER_PAIR_107_PRODUCT_TM=70.9
PRIMER_PAIR_108_PENALTY=2.979016
PRIMER_LEFT_108_PENALTY=2.315468
PRIMER_RIGHT_108_PENALTY=0.663548
PRIMER_LEFT_108_SEQUENCE=CTGATGGTAATGCTGGCCT
PRIMER_RIGHT_108_SEQUENCE=TGAATCCCACTTCCCATTCT
PRIMER_LEFT_108=264,19
PRIMER_RIGHT_108=361,20
PRIMER_LEFT_108_TM=58.685
PRIMER_RIGHT_108_TM=59.336
PRIMER_LEFT_108_GC_PERCENT=52.632
PRIMER_RIGHT_108_GC_PERCENT=45.000
PRIMER_LEFT_108_SELF_ANY=4.00
PRIMER_RIGHT_108_SELF_ANY=4.00
PRIMER_LEFT_108_SELF_END=2.00
PRIMER_RIGHT_108_SELF_END=0.00
PRIMER_LEFT_108_END_STABILITY=10.9000
PRIMER_RIGHT_108_END_STABILITY=6.6000
PRIMER_PAIR_108_COMPL_ANY=5.00
PRIMER_PAIR_108_COMPL_END=2.00
PRIMER_PAIR_108_PRODUCT_SIZE=98
PRIMER_PAIR_108_PRODUCT_TM=74.7
PRIMER_PAIR_109_PENALTY=2.990855
PRIMER_LEFT_109_PENALTY=1.107745
PRIMER_RIGHT_109_PENALTY=1.883110
PRIMER_LEFT_109_SEQUENCE=CCTTCTCGTTCCTGACAAGTG
PRIMER_RIGHT_109_SEQUENCE=ACGGTGTGCCAGTGAAGAT
PRIMER_LEFT_109=577,21
PRIMER_RIGHT_109=657,19
PRIMER_LEFT_109_TM=59.892
PRIMER_RIGHT_109_TM=59.117
PRIMER_LEFT_109_GC_PERCENT=52.381
PRIMER_RIGHT_109_GC_PERCENT=52.632
PRIMER_LEFT_109_SELF_ANY=4.00
PRIMER_RIGHT_109_SELF_ANY=3.00
PRIMER_LEFT_109_SELF_END=2.00
PRIMER_RIGHT_109_SELF_END=2.00
PRIMER_LEFT_109_END_STABILITY=6.7000
PRIMER_RIGHT_109_END_STABILITY=6.6000
PRIMER_PAIR_109_COMPL_ANY=5.00
PRIMER_PAIR_109_COMPL_END=2.00
PRIMER_PAIR_109_PRODUCT_SIZE=81
PRIMER_PAIR_109_PRODUCT_TM=72.2
PRIMER_PAIR_110_PENALTY=3.016344
PRIMER_LEFT_110_PENALTY=1.673871
PRIMER_RIGHT_110_PENALTY=1.342473
PRIMER_LEFT_110_SEQUENCE=TAGAAGCCAACCAACCAGTGA
PRIMER_RIGHT_110_SEQUENCE=CAGCGGTAGGGAATCACAA
PRIMER_LEFT_110=456,21
PRIMER_RIGHT_110=549,19
PRIMER_LEFT_110_TM=60.674
PRIMER_RIGHT_110_TM=59.658
PRIMER_LEFT_110_GC_PERCENT=47.619
PRIMER_RIGHT_110_GC_PERCENT=52.632
PRIMER_LEFT_110_SELF_ANY=3.00
PRIMER_RIGHT_110_SELF_ANY=3.00
PRIMER_LEFT_110_SELF_END=1.00
PRIMER_RIGHT_110_SELF_END=3.00
PRIMER_LEFT_110_END_STABILITY=6.4000
PRIMER_RIGHT_110_END_STABILITY=7.0000
PRIMER_PAIR_110_COMPL_ANY=4.00
PRIMER_PAIR_110_COMPL_END=2.00
PRIMER_PAIR_110_PRODUCT_SIZE=94
PRIMER_PAIR_110_PRODUCT_TM=77.1
PRIMER_PAIR_111_PENALTY=3.023707
PRIMER_LEFT_111_PENALTY=1.876405
PRIMER_RIGHT_111_PENALTY=1.147302
PRIMER_LEFT_111_SEQUENCE=CCCCAGATTGCCATGTTCT
PRIMER_RIGHT_111_SEQUENCE=ATGGATCTGAATCCCACTTCC
PRIMER_LEFT_111=293,19
PRIMER_RIGHT_111=368,21
PRIMER_LEFT_111_TM=60.876
PRIMER_RIGHT_111_TM=60.147
PRIMER_LEFT_111_GC_PERCENT=52.632
PRIMER_RIGHT_111_GC_PERCENT=47.619
PRIMER_LEFT_111_SELF_ANY=4.00
PRIMER_RIGHT_111_SELF_ANY=5.00
PRIMER_LEFT_111_SELF_END=0.00
PRIMER_RIGHT_111_SELF_END=2.00
PRIMER_LEFT_111_END_STABILITY=6.4000
PRIMER_RIGHT_111_END_STABILITY=8.2000
PRIMER_PAIR_111_COMPL_ANY=5.00
PRIMER_PAIR_111_COMPL_END=0.00
PRIMER_PAIR_111_PRODUCT_SIZE=76
PRIMER_PAIR_111_PRODUCT_TM=72.0
PRIMER_PAIR_112_PENALTY=3.028872
PRIMER_LEFT_112_PENALTY=1.513670
PRIMER_RIGHT_112_PENALTY=1.515201
PRIMER_LEFT_112_SEQUENCE=GGACCAAAACCTGCATTGA
PRIMER_RIGHT_112_SEQUENCE=TTGGTTGGCTTCTACCACATT
PRIMER_LEFT_112=372,19
PRIMER_RIGHT_112=469,21
PRIMER_LEFT_112_TM=59.486
PRIMER_RIGHT_112_TM=59.485
PRIMER_LEFT_112_GC_PERCENT=47.368
PRIMER_RIGHT_112_GC_PERCENT=42.857
PRIMER_LEFT_112_SELF_ANY=4.00
PRIMER_RIGHT_112_SELF_ANY=4.00
PRIMER_LEFT_112_SELF_END=1.00
PRIMER_RIGHT_112_SELF_END=1.00
PRIMER_LEFT_112_END_STABILITY=6.9000
PRIMER_RIGHT_112_END_STABILITY=6.6000
PRIMER_PAIR_112_COMPL_ANY=5.00
PRIMER_PAIR_112_COMPL_END=1.00
PRIMER_PAIR_112_PRODUCT_SIZE=98
PRIMER_PAIR_112_PRODUCT_TM=73.4
PRIMER_PAIR_113_PENALTY=3.039862
PRIMER_LEFT_113_PENALTY=2.376315
PRIMER_RIGHT_113_PENALTY=0.663548
PRIMER_LEFT_113_SEQUENCE=GGTAATGCTGGCCTGCTG
PRIMER_RIGHT_113_SEQUENCE=CTGAATCCCACTTCCCATTC
PRIMER_LEFT_113=269,18
PRIMER_RIGHT_113=362,20
PRIMER_LEFT_113_TM=60.376
PRIMER_RIGHT_113_TM=59.336
PRIMER_LEFT_113_GC_PERCENT=61.111
PRIMER_RIGHT_113_GC_PERCENT=50.000
PRIMER_LEFT_113_SELF_ANY=6.00
PRIMER_RIGHT_113_SELF_ANY=4.00
PRIMER_LEFT_113_SELF_END=2.00
PRIMER_RIGHT_113_SELF_END=2.00
PRIMER_LEFT_113_END_STABILITY=8.5000
PRIMER_RIGHT_113_END_STABILITY=6.9000
PRIMER_PAIR_113_COMPL_ANY=4.00
PRIMER_PAIR_113_COMPL_END=2.00
PRIMER_PAIR_113_PRODUCT_SIZE=94
PRIMER_PAIR_113_PRODUCT_TM=74.9
PRIMER_PAIR_114_PENALTY=3.050373
PRIMER_LEFT_114_PENALTY=1.654904
PRIMER_RIGHT_114_PENALTY=1.395469
PRIMER_LEFT_114_SEQUENCE=CCATCCAGAACTGGTGCAAG
PRIMER_RIGHT_114_SEQUENCE=CACCAACTAAGCAGCGGTAG
PRIMER_LEFT_114=477,20
PRIMER_RIGHT_114=560,20
PRIMER_LEFT_114_TM=61.655
PRIMER_RIGHT_114_TM=58.605
PRIMER_LEFT_114_GC_PERCENT=55.000
PRIMER_RIGHT_114_GC_PERCENT=55.000
PRIMER_LEFT_114_SELF_ANY=6.00
PRIMER_RIGHT_114_SELF_ANY=3.00
PRIMER_LEFT_114_SELF_END=2.00
PRIMER_RIGHT_114_SELF_END=3.00
PRIMER_LEFT_114_END_STABILITY=8.5000
PRIMER_RIGHT_114_END_STABILITY=6.9000
PRIMER_PAIR_114_COMPL_ANY=5.00
PRIMER_PAIR_114_COMPL_END=1.00
PRIMER_PAIR_114_PRODUCT_SIZE=84
PRIMER_PAIR_114_PRODUCT_TM=76.7
PRIMER_PAIR_115_PENALTY=3.070713
PRIMER_LEFT_115_PENALTY=1.670728
PRIMER_RIGHT_115_PENALTY=1.399985
PRIMER_LEFT_115_SEQUENCE=CCATCCAGAACTGGTGCAA
PRIMER_RIGHT_115_SEQUENCE=CTCACCAACTAAGCAGCGG
PRIMER_LEFT_115=477,19
PRIMER_RIGHT_115=562,19
PRIMER_LEFT_115_TM=60.671
PRIMER_RIGHT_115_TM=59.600
PRIMER_LEFT_115_GC_PERCENT=52.632
PRIMER_RIGHT_115_GC_PERCENT=57.895
PRIMER_LEFT_115_SELF_ANY=6.00
PRIMER_RIGHT_115_SELF_ANY=3.00
PRIMER_LEFT_115_SELF_END=2.00
PRIMER_RIGHT_115_SELF_END=2.00
PRIMER_LEFT_115_END_STABILITY=8.8000
PRIMER_RIGHT_115_END_STABILITY=11.4000
PRIMER_PAIR_115_COMPL_ANY=5.00
PRIMER_PAIR_115_COMPL_END=1.00
PRIMER_PAIR_115_PRODUCT_SIZE=86
PRIMER_PAIR_115_PRODUCT_TM=76.8
PRIMER_PAIR_116_PENALTY=3.099513
PRIMER_LEFT_116_PENALTY=1.659162
PRIMER_RIGHT_116_PENALTY=1.440351
PRIMER_LEFT_116_SEQUENCE=CATGTTCTGTGGCAGACTGA
PRIMER_RIGHT_116_SEQUENCE=TTGGTCCCTGATGGATCTGA
PRIMER_LEFT_116=304,20
PRIMER_RIGHT_116=378,20
PRIMER_LEFT_116_TM=58.341
PRIMER_RIGHT_116_TM=61.440
PRIMER_LEFT_116_GC_PERCENT=50.000
PRIMER_RIGHT_116_GC_PERCENT=50.000
PRIMER_LEFT_116_SELF_ANY=5.00
PRIMER_RIGHT_116_SELF_ANY=5.00
PRIMER_LEFT_116_SELF_END=3.00
PRIMER_RIGHT_116_SELF_END=3.00
PRIMER_LEFT_116_END_STABILITY=6.4000
PRIMER_RIGHT_116_END_STABILITY=6.7000
PRIMER_PAIR_116_COMPL_ANY=4.00
PRIMER_PAIR_116_COMPL_END=1.00
PRIMER_PAIR_116_PRODUCT_SIZE=75
PRIMER_PAIR_116_PRODUCT_TM=71.6
PRIMER_PAIR_117_PENALTY=3.108139
PRIMER_LEFT_117_PENALTY=2.047791
PRIMER_RIGHT_117_PENALTY=1.060348
PRIMER_LEFT_117_SEQUENCE=TTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_117_SEQUENCE=CACCAGTTCTGGATGGTCAC
PRIMER_LEFT_117=415,20
PRIMER_RIGHT_117=492,20
PRIMER_LEFT_117_TM=57.952
PRIMER_RIGHT_117_TM=58.940
PRIMER_LEFT_117_GC_PERCENT=50.000
PRIMER_RIGHT_117_GC_PERCENT=55.000
PRIMER_LEFT_117_SELF_ANY=5.00
PRIMER_RIGHT_117_SELF_ANY=6.00
PRIMER_LEFT_117_SELF_END=1.00
PRIMER_RIGHT_117_SELF_END=3.00
PRIMER_LEFT_117_END_STABILITY=9.7000
PRIMER_RIGHT_117_END_STABILITY=6.1000
PRIMER_PAIR_117_COMPL_ANY=4.00
PRIMER_PAIR_117_COMPL_END=1.00
PRIMER_PAIR_117_PRODUCT_SIZE=78
PRIMER_PAIR_117_PRODUCT_TM=72.7
PRIMER_PAIR_118_PENALTY=3.121455
PRIMER_LEFT_118_PENALTY=1.188029
PRIMER_RIGHT_118_PENALTY=1.933425
PRIMER_LEFT_118_SEQUENCE=ACATGAATGTCCAGAATGGGA
PRIMER_RIGHT_118_SEQUENCE=AGGATGCCTTCCTTGGTATC
PRIMER_LEFT_118=330,21
PRIMER_RIGHT_118=408,20
PRIMER_LEFT_118_TM=60.188
PRIMER_RIGHT_118_TM=58.067
PRIMER_LEFT_118_GC_PERCENT=42.857
PRIMER_RIGHT_118_GC_PERCENT=50.000
PRIMER_LEFT_118_SELF_ANY=6.00
PRIMER_RIGHT_118_SELF_ANY=7.00
PRIMER_LEFT_118_SELF_END=1.00
PRIMER_RIGHT_118_SELF_END=2.00
PRIMER_LEFT_118_END_STABILITY=9.7000
PRIMER_RIGHT_118_END_STABILITY=5.3000
PRIMER_PAIR_118_COMPL_ANY=3.00
PRIMER_PAIR_118_COMPL_END=2.00
PRIMER_PAIR_118_PRODUCT_SIZE=79
PRIMER_PAIR_118_PRODUCT_TM=71.5
PRIMER_PAIR_119_PENALTY=3.124455
PRIMER_LEFT_119_PENALTY=1.224915
PRIMER_RIGHT_119_PENALTY=1.899540
PRIMER_LEFT_119_SEQUENCE=CGTTCCTGACAAGTGCAAAT
PRIMER_RIGHT_119_SEQUENCE=GACGGTGTGCCAGTGAAGA
PRIMER_LEFT_119=583,20
PRIMER_RIGHT_119=658,19
PRIMER_LEFT_119_TM=58.775
PRIMER_RIGHT_119_TM=60.900
PRIMER_LEFT_119_GC_PERCENT=45.000
PRIMER_RIGHT_119_GC_PERCENT=57.895
PRIMER_LEFT_119_SELF_ANY=4.00
PRIMER_RIGHT_119_SELF_ANY=3.00
PRIMER_LEFT_119_SELF_END=2.00
PRIMER_RIGHT_119_SELF_END=0.00
PRIMER_LEFT_119_END_STABILITY=7.2000
PRIMER_RIGHT_119_END_STABILITY=6.7000
PRIMER_PAIR_119_COMPL_ANY=5.00
PRIMER_PAIR_119_COMPL_END=1.00
PRIMER_PAIR_119_PRODUCT_SIZE=76
PRIMER_PAIR_119_PRODUCT_TM=72.0
PRIMER_PAIR_120_PENALTY=3.126172
PRIMER_LEFT_120_PENALTY=1.546028
PRIMER_RIGHT_120_PENALTY=1.580144
PRIMER_LEFT_120_SEQUENCE=TGTTTGCGAAACTCATCTTCA
PRIMER_RIGHT_120_SEQUENCE=CAGCAACATGCCGTAGTCA
PRIMER_LEFT_120=625,21
PRIMER_RIGHT_120=715,19
PRIMER_LEFT_120_TM=59.454
PRIMER_RIGHT_120_TM=59.420
PRIMER_LEFT_120_GC_PERCENT=38.095
PRIMER_RIGHT_120_GC_PERCENT=52.632
PRIMER_LEFT_120_SELF_ANY=6.00
PRIMER_RIGHT_120_SELF_ANY=4.00
PRIMER_LEFT_120_SELF_END=1.00
PRIMER_RIGHT_120_SELF_END=2.00
PRIMER_LEFT_120_END_STABILITY=7.0000
PRIMER_RIGHT_120_END_STABILITY=6.4000
PRIMER_PAIR_120_COMPL_ANY=4.00
PRIMER_PAIR_120_COMPL_END=1.00
PRIMER_PAIR_120_PRODUCT_SIZE=91
PRIMER_PAIR_120_PRODUCT_TM=73.6
PRIMER_PAIR_121_PENALTY=3.144101
PRIMER_LEFT_121_PENALTY=1.630431
PRIMER_RIGHT_121_PENALTY=1.513670
PRIMER_LEFT_121_SEQUENCE=TGGCAGACTGAACATGCAC
PRIMER_RIGHT_121_SEQUENCE=TCAATGCAGGTTTTGGTCC
PRIMER_LEFT_121=313,19
PRIMER_RIGHT_121=390,19
PRIMER_LEFT_121_TM=59.370
PRIMER_RIGHT_121_TM=59.486
PRIMER_LEFT_121_GC_PERCENT=52.632
PRIMER_RIGHT_121_GC_PERCENT=47.368
PRIMER_LEFT_121_SELF_ANY=5.00
PRIMER_RIGHT_121_SELF_ANY=4.00
PRIMER_LEFT_121_SELF_END=2.00
PRIMER_RIGHT_121_SELF_END=3.00
PRIMER_LEFT_121_END_STABILITY=8.2000
PRIMER_RIGHT_121_END_STABILITY=9.1000
PRIMER_PAIR_121_COMPL_ANY=6.00
PRIMER_PAIR_121_COMPL_END=3.00
PRIMER_PAIR_121_PRODUCT_SIZE=78
PRIMER_PAIR_121_PRODUCT_TM=71.7
PRIMER_PAIR_122_PENALTY=3.180910
PRIMER_LEFT_122_PENALTY=1.395469
PRIMER_RIGHT_122_PENALTY=1.785441
PRIMER_LEFT_122_SEQUENCE=CTACCGCTGCTTAGTTGGTG
PRIMER_RIGHT_122_SEQUENCE=ACATCCATCCTCTCCTGGTGT
PRIMER_LEFT_122=541,20
PRIMER_RIGHT_122=627,21
PRIMER_LEFT_122_TM=58.605
PRIMER_RIGHT_122_TM=60.785
PRIMER_LEFT_122_GC_PERCENT=55.000
PRIMER_RIGHT_122_GC_PERCENT=52.381
PRIMER_LEFT_122_SELF_ANY=3.00
PRIMER_RIGHT_122_SELF_ANY=4.00
PRIMER_LEFT_122_SELF_END=0.00
PRIMER_RIGHT_122_SELF_END=1.00
PRIMER_LEFT_122_END_STABILITY=8.2000
PRIMER_RIGHT_122_END_STABILITY=7.6000
PRIMER_PAIR_122_COMPL_ANY=3.00
PRIMER_PAIR_122_COMPL_END=0.00
PRIMER_PAIR_122_PRODUCT_SIZE=87
PRIMER_PAIR_122_PRODUCT_TM=72.3
PRIMER_PAIR_123_PENALTY=3.191646
PRIMER_LEFT_123_PENALTY=1.781455
PRIMER_RIGHT_123_PENALTY=1.410191
PRIMER_LEFT_123_SEQUENCE=CCAACCAACCAGTGACCAT
PRIMER_RIGHT_123_SEQUENCE=ATCACAAAGTGGGGATGGGT
PRIMER_LEFT_123=462,19
PRIMER_RIGHT_123=537,20
PRIMER_LEFT_123_TM=59.219
PRIMER_RIGHT_123_TM=61.410
PRIMER_LEFT_123_GC_PERCENT=52.632
PRIMER_RIGHT_123_GC_PERCENT=50.000
PRIMER_LEFT_123_SELF_ANY=3.00
PRIMER_RIGHT_123_SELF_ANY=4.00
PRIMER_LEFT_123_SELF_END=2.00
PRIMER_RIGHT_123_SELF_END=0.00
PRIMER_LEFT_123_END_STABILITY=7.8000
PRIMER_RIGHT_123_END_STABILITY=9.4000
PRIMER_PAIR_123_COMPL_ANY=4.00
PRIMER_PAIR_123_COMPL_END=3.00
PRIMER_PAIR_123_PRODUCT_SIZE=76
PRIMER_PAIR_123_PRODUCT_TM=75.7
PRIMER_PAIR_124_PENALTY=3.198555
PRIMER_LEFT_124_PENALTY=2.051254
PRIMER_RIGHT_124_PENALTY=1.147302
PRIMER_LEFT_124_SEQUENCE=AACCCCAGATTGCCATGTTC
PRIMER_RIGHT_124_SEQUENCE=GATCTGAATCCCACTTCCCAT
PRIMER_LEFT_124=291,20
PRIMER_RIGHT_124=365,21
PRIMER_LEFT_124_TM=62.051
PRIMER_RIGHT_124_TM=60.147
PRIMER_LEFT_124_GC_PERCENT=50.000
PRIMER_RIGHT_124_GC_PERCENT=47.619
PRIMER_LEFT_124_SELF_ANY=4.00
PRIMER_RIGHT_124_SELF_ANY=4.00
PRIMER_LEFT_124_SELF_END=0.00
PRIMER_RIGHT_124_SELF_END=2.00
PRIMER_LEFT_124_END_STABILITY=6.7000
PRIMER_RIGHT_124_END_STABILITY=9.6000
PRIMER_PAIR_124_COMPL_ANY=5.00
PRIMER_PAIR_124_COMPL_END=0.00
PRIMER_PAIR_124_PRODUCT_SIZE=75
PRIMER_PAIR_124_PRODUCT_TM=71.6
PRIMER_PAIR_125_PENALTY=3.212911
PRIMER_LEFT_125_PENALTY=2.124336
PRIMER_RIGHT_125_PENALTY=1.088575
PRIMER_LEFT_125_SEQUENCE=TCGCCAAAGAGACATGCAG
PRIMER_RIGHT_125_SEQUENCE=CCCCTCGGAACTTGTCAAT
PRIMER_LEFT_125=657,19
PRIMER_RIGHT_125=743,19
PRIMER_LEFT_125_TM=61.124
PRIMER_RIGHT_125_TM=59.911
PRIMER_LEFT_125_GC_PERCENT=52.632
PRIMER_RIGHT_125_GC_PERCENT=52.632
PRIMER_LEFT_125_SELF_ANY=4.00
PRIMER_RIGHT_125_SELF_ANY=5.00
PRIMER_LEFT_125_SELF_END=2.00
PRIMER_RIGHT_125_SELF_END=3.00
PRIMER_LEFT_125_END_STABILITY=8.5000
PRIMER_RIGHT_125_END_STABILITY=6.9000
PRIMER_PAIR_125_COMPL_ANY=4.00
PRIMER_PAIR_125_COMPL_END=0.00
PRIMER_PAIR_125_PRODUCT_SIZE=87
PRIMER_PAIR_125_PRODUCT_TM=74.7
PRIMER_PAIR_126_PENALTY=3.218494
PRIMER_LEFT_126_PENALTY=1.399985
PRIMER_RIGHT_126_PENALTY=1.818509
PRIMER_LEFT_126_SEQUENCE=CCGCTGCTTAGTTGGTGAG
PRIMER_RIGHT_126_SEQUENCE=CGCAAACATCCATCCTCTC
PRIMER_LEFT_126=544,19
PRIMER_RIGHT_126=632,19
PRIMER_LEFT_126_TM=59.600
PRIMER_RIGHT_126_TM=59.181
PRIMER_LEFT_126_GC_PERCENT=57.895
PRIMER_RIGHT_126_GC_PERCENT=52.632
PRIMER_LEFT_126_SELF_ANY=3.00
PRIMER_RIGHT_126_SELF_ANY=2.00
PRIMER_LEFT_126_SELF_END=0.00
PRIMER_RIGHT_126_SELF_END=0.00
PRIMER_LEFT_126_END_STABILITY=6.4000
PRIMER_RIGHT_126_END_STABILITY=6.4000
PRIMER_PAIR_126_COMPL_ANY=3.00
PRIMER_PAIR_126_COMPL_END=3.00
PRIMER_PAIR_126_PRODUCT_SIZE=89
PRIMER_PAIR_126_PRODUCT_TM=73.0
PRIMER_PAIR_127_PENALTY=3.234917
PRIMER_LEFT_127_PENALTY=1.630431
PRIMER_RIGHT_127_PENALTY=1.604487
PRIMER_LEFT_127_SEQUENCE=TGTGGCAGACTGAACATGC
PRIMER_RIGHT_127_SEQUENCE=AATGCAGGTTTTGGTCCCT
PRIMER_LEFT_127=311,19
PRIMER_RIGHT_127=388,19
PRIMER_LEFT_127_TM=59.370
PRIMER_RIGHT_127_TM=59.396
PRIMER_LEFT_127_GC_PERCENT=52.632
PRIMER_RIGHT_127_GC_PERCENT=47.368
PRIMER_LEFT_127_SELF_ANY=5.00
PRIMER_RIGHT_127_SELF_ANY=4.00
PRIMER_LEFT_127_SELF_END=2.00
PRIMER_RIGHT_127_SELF_END=0.00
PRIMER_LEFT_127_END_STABILITY=8.4000
PRIMER_RIGHT_127_END_STABILITY=9.4000
PRIMER_PAIR_127_COMPL_ANY=5.00
PRIMER_PAIR_127_COMPL_END=2.00
PRIMER_PAIR_127_PRODUCT_SIZE=78
PRIMER_PAIR_127_PRODUCT_TM=71.7
PRIMER_PAIR_128_PENALTY=3.239449
PRIMER_LEFT_128_PENALTY=1.810378
PRIMER_RIGHT_128_PENALTY=1.429072
PRIMER_LEFT_128_SEQUENCE=CATCCAGAACTGGTGCAAGC
PRIMER_RIGHT_128_SEQUENCE=AAACTCACCAACTAAGCAGCG
PRIMER_LEFT_128=478,20
PRIMER_RIGHT_128=565,21
PRIMER_LEFT_128_TM=61.810
PRIMER_RIGHT_128_TM=59.571
PRIMER_LEFT_128_GC_PERCENT=55.000
PRIMER_RIGHT_128_GC_PERCENT=47.619
PRIMER_LEFT_128_SELF_ANY=6.00
PRIMER_RIGHT_128_SELF_ANY=3.00
PRIMER_LEFT_128_SELF_END=2.00
PRIMER_RIGHT_128_SELF_END=2.00
PRIMER_LEFT_128_END_STABILITY=8.5000
PRIMER_RIGHT_128_END_STABILITY=10.2000
PRIMER_PAIR_128_COMPL_ANY=5.00
PRIMER_PAIR_128_COMPL_END=1.00
PRIMER_PAIR_128_PRODUCT_SIZE=88
PRIMER_PAIR_128_PRODUCT_TM=75.9
PRIMER_PAIR_129_PENALTY=3.247580
PRIMER_LEFT_129_PENALTY=1.429072
PRIMER_RIGHT_129_PENALTY=1.818509
PRIMER_LEFT_129_SEQUENCE=CGCTGCTTAGTTGGTGAGTTT
PRIMER_RIGHT_129_SEQUENCE=TCGCAAACATCCATCCTCT
PRIMER_LEFT_129=545,21
PRIMER_RIGHT_129=633,19
PRIMER_LEFT_129_TM=59.571
PRIMER_RIGHT_129_TM=59.181
PRIMER_LEFT_129_GC_PERCENT=47.619
PRIMER_RIGHT_129_GC_PERCENT=47.368
PRIMER_LEFT_129_SELF_ANY=3.00
PRIMER_RIGHT_129_SELF_ANY=2.00
PRIMER_LEFT_129_SELF_END=0.00
PRIMER_RIGHT_129_SELF_END=0.00
PRIMER_LEFT_129_END_STABILITY=6.7000
PRIMER_RIGHT_129_END_STABILITY=7.9000
PRIMER_PAIR_129_COMPL_ANY=4.00
PRIMER_PAIR_129_COMPL_END=3.00
PRIMER_PAIR_129_PRODUCT_SIZE=89
PRIMER_PAIR_129_PRODUCT_TM=72.5
PRIMER_PAIR_130_PENALTY=3.275628
PRIMER_LEFT_130_PENALTY=0.860239
PRIMER_RIGHT_130_PENALTY=2.415388
PRIMER_LEFT_130_SEQUENCE=CAGATCCATCAGGGACCAAA
PRIMER_RIGHT_130_SEQUENCE=TCTACCACATTGGTGATCTGC
PRIMER_LEFT_130=360,20
PRIMER_RIGHT_130=459,21
PRIMER_LEFT_130_TM=60.860
PRIMER_RIGHT_130_TM=58.585
PRIMER_LEFT_130_GC_PERCENT=50.000
PRIMER_RIGHT_130_GC_PERCENT=47.619
PRIMER_LEFT_130_SELF_ANY=5.00
PRIMER_RIGHT_130_SELF_ANY=6.00
PRIMER_LEFT_130_SELF_END=0.00
PRIMER_RIGHT_130_SELF_END=2.00
PRIMER_LEFT_130_END_STABILITY=8.8000
PRIMER_RIGHT_130_END_STABILITY=8.2000
PRIMER_PAIR_130_COMPL_ANY=6.00
PRIMER_PAIR_130_COMPL_END=2.00
PRIMER_PAIR_130_PRODUCT_SIZE=100
PRIMER_PAIR_130_PRODUCT_TM=73.6
PRIMER_PAIR_131_PENALTY=3.277129
PRIMER_LEFT_131_PENALTY=2.613581
PRIMER_RIGHT_131_PENALTY=0.663548
PRIMER_LEFT_131_SEQUENCE=CTGGCTGAACCCCAGATTG
PRIMER_RIGHT_131_SEQUENCE=TCTGAATCCCACTTCCCATT
PRIMER_LEFT_131=284,19
PRIMER_RIGHT_131=363,20
PRIMER_LEFT_131_TM=61.614
PRIMER_RIGHT_131_TM=59.336
PRIMER_LEFT_131_GC_PERCENT=57.895
PRIMER_RIGHT_131_GC_PERCENT=45.000
PRIMER_LEFT_131_SELF_ANY=4.00
PRIMER_RIGHT_131_SELF_ANY=3.00
PRIMER_LEFT_131_SELF_END=2.00
PRIMER_RIGHT_131_SELF_END=1.00
PRIMER_LEFT_131_END_STABILITY=6.9000
PRIMER_RIGHT_131_END_STABILITY=8.4000
PRIMER_PAIR_131_COMPL_ANY=4.00
PRIMER_PAIR_131_COMPL_END=2.00
PRIMER_PAIR_131_PRODUCT_SIZE=80
PRIMER_PAIR_131_PRODUCT_TM=72.9
PRIMER_PAIR_132_PENALTY=3.298886
PRIMER_LEFT_132_PENALTY=1.446550
PRIMER_RIGHT_132_PENALTY=1.852336
PRIMER_LEFT_132_SEQUENCE=TACCGCTGCTTAGTTGGTGAG
PRIMER_RIGHT_132_SEQUENCE=CCATCCTCTCCTGGTGTAAGA
PRIMER_LEFT_132=542,21
PRIMER_RIGHT_132=623,21
PRIMER_LEFT_132_TM=60.447
PRIMER_RIGHT_132_TM=59.148
PRIMER_LEFT_132_GC_PERCENT=52.381
PRIMER_RIGHT_132_GC_PERCENT=52.381
PRIMER_LEFT_132_SELF_ANY=3.00
PRIMER_RIGHT_132_SELF_ANY=3.00
PRIMER_LEFT_132_SELF_END=0.00
PRIMER_RIGHT_132_SELF_END=3.00
PRIMER_LEFT_132_END_STABILITY=6.4000
PRIMER_RIGHT_132_END_STABILITY=6.0000
PRIMER_PAIR_132_COMPL_ANY=4.00
PRIMER_PAIR_132_COMPL_END=0.00
PRIMER_PAIR_132_PRODUCT_SIZE=82
PRIMER_PAIR_132_PRODUCT_TM=72.1
PRIMER_PAIR_133_PENALTY=3.298886
PRIMER_LEFT_133_PENALTY=1.446550
PRIMER_RIGHT_133_PENALTY=1.852336
PRIMER_LEFT_133_SEQUENCE=CTACCGCTGCTTAGTTGGTGA
PRIMER_RIGHT_133_SEQUENCE=TCCATCCTCTCCTGGTGTAAG
PRIMER_LEFT_133=541,21
PRIMER_RIGHT_133=624,21
PRIMER_LEFT_133_TM=60.447
PRIMER_RIGHT_133_TM=59.148
PRIMER_LEFT_133_GC_PERCENT=52.381
PRIMER_RIGHT_133_GC_PERCENT=52.381
PRIMER_LEFT_133_SELF_ANY=3.00
PRIMER_RIGHT_133_SELF_ANY=3.00
PRIMER_LEFT_133_SELF_END=1.00
PRIMER_RIGHT_133_SELF_END=1.00
PRIMER_LEFT_133_END_STABILITY=7.9000
PRIMER_RIGHT_133_END_STABILITY=5.7000
PRIMER_PAIR_133_COMPL_ANY=4.00
PRIMER_PAIR_133_COMPL_END=0.00
PRIMER_PAIR_133_PRODUCT_SIZE=84
PRIMER_PAIR_133_PRODUCT_TM=72.3
PRIMER_PAIR_134_PENALTY=3.306030
PRIMER_LEFT_134_PENALTY=1.675600
PRIMER_RIGHT_134_PENALTY=1.630431
PRIMER_LEFT_134_SEQUENCE=TGGAGGTACCCACTGATGG
PRIMER_RIGHT_134_SEQUENCE=TGCATGTTCAGTCTGCCAC
PRIMER_LEFT_134=252,19
PRIMER_RIGHT_134=330,19
PRIMER_LEFT_134_TM=59.324
PRIMER_RIGHT_134_TM=59.370
PRIMER_LEFT_134_GC_PERCENT=57.895
PRIMER_RIGHT_134_GC_PERCENT=52.632
PRIMER_LEFT_134_SELF_ANY=6.00
PRIMER_RIGHT_134_SELF_ANY=5.00
PRIMER_LEFT_134_SELF_END=3.00
PRIMER_RIGHT_134_SELF_END=3.00
PRIMER_LEFT_134_END_STABILITY=8.1000
PRIMER_RIGHT_134_END_STABILITY=9.4000
PRIMER_PAIR_134_COMPL_ANY=5.00
PRIMER_PAIR_134_COMPL_END=2.00
PRIMER_PAIR_134_PRODUCT_SIZE=79
PRIMER_PAIR_134_PRODUCT_TM=74.6
PRIMER_PAIR_135_PENALTY=3.310502
PRIMER_LEFT_135_PENALTY=2.051254
PRIMER_RIGHT_135_PENALTY=1.259248
PRIMER_LEFT_135_SEQUENCE=GAACCCCAGATTGCCATGTT
PRIMER_RIGHT_135_SEQUENCE=GGTCCCTGATGGATCTGAAT
PRIMER_LEFT_135=290,20
PRIMER_RIGHT_135=376,20
PRIMER_LEFT_135_TM=62.051
PRIMER_RIGHT_135_TM=58.741
PRIMER_LEFT_135_GC_PERCENT=50.000
PRIMER_RIGHT_135_GC_PERCENT=50.000
PRIMER_LEFT_135_SELF_ANY=4.00
PRIMER_RIGHT_135_SELF_ANY=5.00
PRIMER_LEFT_135_SELF_END=2.00
PRIMER_RIGHT_135_SELF_END=2.00
PRIMER_LEFT_135_END_STABILITY=6.6000
PRIMER_RIGHT_135_END_STABILITY=6.9000
PRIMER_PAIR_135_COMPL_ANY=5.00
PRIMER_PAIR_135_COMPL_END=1.00
PRIMER_PAIR_135_PRODUCT_SIZE=87
PRIMER_PAIR_135_PRODUCT_TM=73.7
PRIMER_PAIR_136_PENALTY=3.324260
PRIMER_LEFT_136_PENALTY=0.663548
PRIMER_RIGHT_136_PENALTY=2.660713
PRIMER_LEFT_136_SEQUENCE=GAATGGGAAGTGGGATTCAG
PRIMER_RIGHT_136_SEQUENCE=GTTCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_136=343,20
PRIMER_RIGHT_136=437,22
PRIMER_LEFT_136_TM=59.336
PRIMER_RIGHT_136_TM=60.661
PRIMER_LEFT_136_GC_PERCENT=50.000
PRIMER_RIGHT_136_GC_PERCENT=50.000
PRIMER_LEFT_136_SELF_ANY=4.00
PRIMER_RIGHT_136_SELF_ANY=4.00
PRIMER_LEFT_136_SELF_END=1.00
PRIMER_RIGHT_136_SELF_END=3.00
PRIMER_LEFT_136_END_STABILITY=7.0000
PRIMER_RIGHT_136_END_STABILITY=10.0000
PRIMER_PAIR_136_COMPL_ANY=5.00
PRIMER_PAIR_136_COMPL_END=0.00
PRIMER_PAIR_136_PRODUCT_SIZE=95
PRIMER_PAIR_136_PRODUCT_TM=73.4
PRIMER_PAIR_137_PENALTY=3.324495
PRIMER_LEFT_137_PENALTY=1.811785
PRIMER_RIGHT_137_PENALTY=1.512710
PRIMER_LEFT_137_SEQUENCE=GGTACCCACTGATGGTAATGC
PRIMER_RIGHT_137_SEQUENCE=CTTCCCATTCTGGACATTCA
PRIMER_LEFT_137=256,21
PRIMER_RIGHT_137=352,20
PRIMER_LEFT_137_TM=59.188
PRIMER_RIGHT_137_TM=58.487
PRIMER_LEFT_137_GC_PERCENT=52.381
PRIMER_RIGHT_137_GC_PERCENT=45.000
PRIMER_LEFT_137_SELF_ANY=6.00
PRIMER_RIGHT_137_SELF_ANY=3.00
PRIMER_LEFT_137_SELF_END=2.00
PRIMER_RIGHT_137_SELF_END=2.00
PRIMER_LEFT_137_END_STABILITY=8.4000
PRIMER_RIGHT_137_END_STABILITY=6.9000
PRIMER_PAIR_137_COMPL_ANY=5.00
PRIMER_PAIR_137_COMPL_END=2.00
PRIMER_PAIR_137_PRODUCT_SIZE=97
PRIMER_PAIR_137_PRODUCT_TM=75.3
PRIMER_PAIR_138_PENALTY=3.329952
PRIMER_LEFT_138_PENALTY=2.212679
PRIMER_RIGHT_138_PENALTY=1.117273
PRIMER_LEFT_138_SEQUENCE=CCGTCGCCAAAGAGACAT
PRIMER_RIGHT_138_SEQUENCE=CTCTACCCCTCGGAACTTGTC
PRIMER_LEFT_138=654,18
PRIMER_RIGHT_138=748,21
PRIMER_LEFT_138_TM=59.787
PRIMER_RIGHT_138_TM=60.117
PRIMER_LEFT_138_GC_PERCENT=55.556
PRIMER_RIGHT_138_GC_PERCENT=57.143
PRIMER_LEFT_138_SELF_ANY=3.00
PRIMER_RIGHT_138_SELF_ANY=3.00
PRIMER_LEFT_138_SELF_END=2.00
PRIMER_RIGHT_138_SELF_END=1.00
PRIMER_LEFT_138_END_STABILITY=6.3000
PRIMER_RIGHT_138_END_STABILITY=6.7000
PRIMER_PAIR_138_COMPL_ANY=4.00
PRIMER_PAIR_138_COMPL_END=3.00
PRIMER_PAIR_138_PRODUCT_SIZE=95
PRIMER_PAIR_138_PRODUCT_TM=75.6
PRIMER_PAIR_139_PENALTY=3.332276
PRIMER_LEFT_139_PENALTY=0.663548
PRIMER_RIGHT_139_PENALTY=2.668729
PRIMER_LEFT_139_SEQUENCE=AGAATGGGAAGTGGGATTCA
PRIMER_RIGHT_139_SEQUENCE=CAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_139=342,20
PRIMER_RIGHT_139=434,21
PRIMER_LEFT_139_TM=59.336
PRIMER_RIGHT_139_TM=58.331
PRIMER_LEFT_139_GC_PERCENT=45.000
PRIMER_RIGHT_139_GC_PERCENT=47.619
PRIMER_LEFT_139_SELF_ANY=4.00
PRIMER_RIGHT_139_SELF_ANY=5.00
PRIMER_LEFT_139_SELF_END=1.00
PRIMER_RIGHT_139_SELF_END=3.00
PRIMER_LEFT_139_END_STABILITY=6.9000
PRIMER_RIGHT_139_END_STABILITY=8.4000
PRIMER_PAIR_139_COMPL_ANY=5.00
PRIMER_PAIR_139_COMPL_END=1.00
PRIMER_PAIR_139_PRODUCT_SIZE=93
PRIMER_PAIR_139_PRODUCT_TM=73.3
PRIMER_PAIR_140_PENALTY=3.349242
PRIMER_LEFT_140_PENALTY=1.782887
PRIMER_RIGHT_140_PENALTY=1.566355
PRIMER_LEFT_140_SEQUENCE=AGACCCATCCCCACTTTGT
PRIMER_RIGHT_140_SEQUENCE=GTCAGGAACGAGAAGGGCAT
PRIMER_LEFT_140=516,19
PRIMER_RIGHT_140=592,20
PRIMER_LEFT_140_TM=59.217
PRIMER_RIGHT_140_TM=61.566
PRIMER_LEFT_140_GC_PERCENT=52.632
PRIMER_RIGHT_140_GC_PERCENT=55.000
PRIMER_LEFT_140_SELF_ANY=2.00
PRIMER_RIGHT_140_SELF_ANY=2.00
PRIMER_LEFT_140_SELF_END=1.00
PRIMER_RIGHT_140_SELF_END=2.00
PRIMER_LEFT_140_END_STABILITY=7.0000
PRIMER_RIGHT_140_END_STABILITY=9.6000
PRIMER_PAIR_140_COMPL_ANY=4.00
PRIMER_PAIR_140_COMPL_END=3.00
PRIMER_PAIR_140_PRODUCT_SIZE=77
PRIMER_PAIR_140_PRODUCT_TM=72.9
PRIMER_PAIR_141_PENALTY=3.352040
PRIMER_LEFT_141_PENALTY=1.065099
PRIMER_RIGHT_141_PENALTY=2.286941
PRIMER_LEFT_141_SEQUENCE=TACACCAGGAGAGGATGGATG
PRIMER_RIGHT_141_SEQUENCE=CCGTAGTCATGCAAGTTGGTA
PRIMER_LEFT_141=606,21
PRIMER_RIGHT_141=705,21
PRIMER_LEFT_141_TM=59.935
PRIMER_RIGHT_141_TM=58.713
PRIMER_LEFT_141_GC_PERCENT=52.381
PRIMER_RIGHT_141_GC_PERCENT=47.619
PRIMER_LEFT_141_SELF_ANY=3.00
PRIMER_RIGHT_141_SELF_ANY=5.00
PRIMER_LEFT_141_SELF_END=0.00
PRIMER_RIGHT_141_SELF_END=2.00
PRIMER_LEFT_141_END_STABILITY=8.1000
PRIMER_RIGHT_141_END_STABILITY=7.2000
PRIMER_PAIR_141_COMPL_ANY=4.00
PRIMER_PAIR_141_COMPL_END=0.00
PRIMER_PAIR_141_PRODUCT_SIZE=100
PRIMER_PAIR_141_PRODUCT_TM=74.4
PRIMER_PAIR_142_PENALTY=3.399919
PRIMER_LEFT_142_PENALTY=1.544828
PRIMER_RIGHT_142_PENALTY=1.855091
PRIMER_LEFT_142_SEQUENCE=ACCGCTGCTTAGTTGGTGA
PRIMER_RIGHT_142_SEQUENCE=GAGTTTCGCAAACATCCATC
PRIMER_LEFT_142=543,19
PRIMER_RIGHT_142=638,20
PRIMER_LEFT_142_TM=59.455
PRIMER_RIGHT_142_TM=58.145
PRIMER_LEFT_142_GC_PERCENT=52.632
PRIMER_RIGHT_142_GC_PERCENT=45.000
PRIMER_LEFT_142_SELF_ANY=3.00
PRIMER_RIGHT_142_SELF_ANY=6.00
PRIMER_LEFT_142_SELF_END=1.00
PRIMER_RIGHT_142_SELF_END=0.00
PRIMER_LEFT_142_END_STABILITY=7.9000
PRIMER_RIGHT_142_END_STABILITY=8.1000
PRIMER_PAIR_142_COMPL_ANY=4.00
PRIMER_PAIR_142_COMPL_END=3.00
PRIMER_PAIR_142_PRODUCT_SIZE=96
PRIMER_PAIR_142_PRODUCT_TM=72.9
PRIMER_PAIR_143_PENALTY=3.414813
PRIMER_LEFT_143_PENALTY=1.968263
PRIMER_RIGHT_143_PENALTY=1.446550
PRIMER_LEFT_143_SEQUENCE=CCAACCAGTGACCATCCAGA
PRIMER_RIGHT_143_SEQUENCE=TCACCAACTAAGCAGCGGTAG
PRIMER_LEFT_143=466,20
PRIMER_RIGHT_143=561,21
PRIMER_LEFT_143_TM=61.968
PRIMER_RIGHT_143_TM=60.447
PRIMER_LEFT_143_GC_PERCENT=55.000
PRIMER_RIGHT_143_GC_PERCENT=52.381
PRIMER_LEFT_143_SELF_ANY=3.00
PRIMER_RIGHT_143_SELF_ANY=3.00
PRIMER_LEFT_143_SELF_END=2.00
PRIMER_RIGHT_143_SELF_END=3.00
PRIMER_LEFT_143_END_STABILITY=8.2000
PRIMER_RIGHT_143_END_STABILITY=6.9000
PRIMER_PAIR_143_COMPL_ANY=4.00
PRIMER_PAIR_143_COMPL_END=0.00
PRIMER_PAIR_143_PRODUCT_SIZE=96
PRIMER_PAIR_143_PRODUCT_TM=77.1
PRIMER_PAIR_144_PENALTY=3.433919
PRIMER_LEFT_144_PENALTY=2.182723
PRIMER_RIGHT_144_PENALTY=1.251196
PRIMER_LEFT_144_SEQUENCE=AAGCAGTGCAAGACCCATC
PRIMER_RIGHT_144_SEQUENCE=ACGAGAAGGGCATCACTTACA
PRIMER_LEFT_144=506,19
PRIMER_RIGHT_144=585,21
PRIMER_LEFT_144_TM=58.817
PRIMER_RIGHT_144_TM=59.749
PRIMER_LEFT_144_GC_PERCENT=52.632
PRIMER_RIGHT_144_GC_PERCENT=47.619
PRIMER_LEFT_144_SELF_ANY=5.00
PRIMER_RIGHT_144_SELF_ANY=4.00
PRIMER_LEFT_144_SELF_END=0.00
PRIMER_RIGHT_144_SELF_END=0.00
PRIMER_LEFT_144_END_STABILITY=8.1000
PRIMER_RIGHT_144_END_STABILITY=6.0000
PRIMER_PAIR_144_COMPL_ANY=5.00
PRIMER_PAIR_144_COMPL_END=2.00
PRIMER_PAIR_144_PRODUCT_SIZE=80
PRIMER_PAIR_144_PRODUCT_TM=72.9
PRIMER_PAIR_145_PENALTY=3.452176
PRIMER_LEFT_145_PENALTY=2.389824
PRIMER_RIGHT_145_PENALTY=1.062353
PRIMER_LEFT_145_SEQUENCE=TTGATACCAAGGAAGGCATC
PRIMER_RIGHT_145_SEQUENCE=CTGGATGGTCACTGGTTGG
PRIMER_LEFT_145=387,20
PRIMER_RIGHT_145=484,19
PRIMER_LEFT_145_TM=57.610
PRIMER_RIGHT_145_TM=59.938
PRIMER_LEFT_145_GC_PERCENT=45.000
PRIMER_RIGHT_145_GC_PERCENT=57.895
PRIMER_LEFT_145_SELF_ANY=6.00
PRIMER_RIGHT_145_SELF_ANY=3.00
PRIMER_LEFT_145_SELF_END=3.00
PRIMER_RIGHT_145_SELF_END=0.00
PRIMER_LEFT_145_END_STABILITY=8.1000
PRIMER_RIGHT_145_END_STABILITY=8.2000
PRIMER_PAIR_145_COMPL_ANY=4.00
PRIMER_PAIR_145_COMPL_END=2.00
PRIMER_PAIR_145_PRODUCT_SIZE=98
PRIMER_PAIR_145_PRODUCT_TM=73.9
PRIMER_PAIR_146_PENALTY=3.461868
PRIMER_LEFT_146_PENALTY=1.533205
PRIMER_RIGHT_146_PENALTY=1.928663
PRIMER_LEFT_146_SEQUENCE=GTGATTCCCTACCGCTGCTT
PRIMER_RIGHT_146_SEQUENCE=TCCATCCTCTCCTGGTGTAA
PRIMER_LEFT_146=533,20
PRIMER_RIGHT_146=624,20
PRIMER_LEFT_146_TM=61.533
PRIMER_RIGHT_146_TM=58.071
PRIMER_LEFT_146_GC_PERCENT=55.000
PRIMER_RIGHT_146_GC_PERCENT=50.000
PRIMER_LEFT_146_SELF_ANY=3.00
PRIMER_RIGHT_146_SELF_ANY=3.00
PRIMER_LEFT_146_SELF_END=0.00
PRIMER_RIGHT_146_SELF_END=1.00
PRIMER_LEFT_146_END_STABILITY=8.5000
PRIMER_RIGHT_146_END_STABILITY=6.0000
PRIMER_PAIR_146_COMPL_ANY=3.00
PRIMER_PAIR_146_COMPL_END=2.00
PRIMER_PAIR_146_PRODUCT_SIZE=92
PRIMER_PAIR_146_PRODUCT_TM=73.0
PRIMER_PAIR_147_PENALTY=3.465550
PRIMER_LEFT_147_PENALTY=2.056428
PRIMER_RIGHT_147_PENALTY=1.409123
PRIMER_LEFT_147_SEQUENCE=CCAAAACCTGCATTGATACC
PRIMER_RIGHT_147_SEQUENCE=ACTGGTTGGTTGGCTTCTACC
PRIMER_LEFT_147=375,20
PRIMER_RIGHT_147=474,21
PRIMER_LEFT_147_TM=57.944
PRIMER_RIGHT_147_TM=60.409
PRIMER_LEFT_147_GC_PERCENT=45.000
PRIMER_RIGHT_147_GC_PERCENT=52.381
PRIMER_LEFT_147_SELF_ANY=4.00
PRIMER_RIGHT_147_SELF_ANY=3.00
PRIMER_LEFT_147_SELF_END=2.00
PRIMER_RIGHT_147_SELF_END=2.00
PRIMER_LEFT_147_END_STABILITY=6.8000
PRIMER_RIGHT_147_END_STABILITY=6.9000
PRIMER_PAIR_147_COMPL_ANY=4.00
PRIMER_PAIR_147_COMPL_END=0.00
PRIMER_PAIR_147_PRODUCT_SIZE=100
PRIMER_PAIR_147_PRODUCT_TM=73.6
PRIMER_PAIR_148_PENALTY=3.469999
PRIMER_LEFT_148_PENALTY=2.214324
PRIMER_RIGHT_148_PENALTY=1.255675
PRIMER_LEFT_148_SEQUENCE=GCAGTGCAAGACCCATCC
PRIMER_RIGHT_148_SEQUENCE=CGAGAAGGGCATCACTTACAA
PRIMER_LEFT_148=508,18
PRIMER_RIGHT_148=584,21
PRIMER_LEFT_148_TM=60.214
PRIMER_RIGHT_148_TM=60.256
PRIMER_LEFT_148_GC_PERCENT=61.111
PRIMER_RIGHT_148_GC_PERCENT=47.619
PRIMER_LEFT_148_SELF_ANY=5.00
PRIMER_RIGHT_148_SELF_ANY=4.00
PRIMER_LEFT_148_SELF_END=0.00
PRIMER_RIGHT_148_SELF_END=2.00
PRIMER_LEFT_148_END_STABILITY=8.1000
PRIMER_RIGHT_148_END_STABILITY=6.0000
PRIMER_PAIR_148_COMPL_ANY=5.00
PRIMER_PAIR_148_COMPL_END=2.00
PRIMER_PAIR_148_PRODUCT_SIZE=77
PRIMER_PAIR_148_PRODUCT_TM=73.4
PRIMER_PAIR_149_PENALTY=3.507898
PRIMER_LEFT_149_PENALTY=1.923751
PRIMER_RIGHT_149_PENALTY=1.584147
PRIMER_LEFT_149_SEQUENCE=ATGTGGTAGAAGCCAACCAA
PRIMER_RIGHT_149_SEQUENCE=ATCACAAAGTGGGGATGGG
PRIMER_LEFT_149=450,20
PRIMER_RIGHT_149=537,19
PRIMER_LEFT_149_TM=58.076
PRIMER_RIGHT_149_TM=60.584
PRIMER_LEFT_149_GC_PERCENT=45.000
PRIMER_RIGHT_149_GC_PERCENT=52.632
PRIMER_LEFT_149_SELF_ANY=4.00
PRIMER_RIGHT_149_SELF_ANY=4.00
PRIMER_LEFT_149_SELF_END=2.00
PRIMER_RIGHT_149_SELF_END=0.00
PRIMER_LEFT_149_END_STABILITY=8.2000
PRIMER_RIGHT_149_END_STABILITY=9.6000
PRIMER_PAIR_149_COMPL_ANY=4.00
PRIMER_PAIR_149_COMPL_END=2.00
PRIMER_PAIR_149_PRODUCT_SIZE=88
PRIMER_PAIR_149_PRODUCT_TM=75.9
PRIMER_PAIR_150_PENALTY=3.511514
PRIMER_LEFT_150_PENALTY=2.071163
PRIMER_RIGHT_150_PENALTY=1.440351
PRIMER_LEFT_150_SEQUENCE=ATTGCCATGTTCTGTGGCAG
PRIMER_RIGHT_150_SEQUENCE=TGGTCCCTGATGGATCTGAA
PRIMER_LEFT_150=299,20
PRIMER_RIGHT_150=377,20
PRIMER_LEFT_150_TM=62.071
PRIMER_RIGHT_150_TM=61.440
PRIMER_LEFT_150_GC_PERCENT=50.000
PRIMER_RIGHT_150_GC_PERCENT=50.000
PRIMER_LEFT_150_SELF_ANY=5.00
PRIMER_RIGHT_150_SELF_ANY=5.00
PRIMER_LEFT_150_SELF_END=3.00
PRIMER_RIGHT_150_SELF_END=1.00
PRIMER_LEFT_150_END_STABILITY=9.7000
PRIMER_RIGHT_150_END_STABILITY=7.0000
PRIMER_PAIR_150_COMPL_ANY=4.00
PRIMER_PAIR_150_COMPL_END=1.00
PRIMER_PAIR_150_PRODUCT_SIZE=79
PRIMER_PAIR_150_PRODUCT_TM=72.0
PRIMER_PAIR_151_PENALTY=3.515712
PRIMER_LEFT_151_PENALTY=1.982507
PRIMER_RIGHT_151_PENALTY=1.533205
PRIMER_LEFT_151_SEQUENCE=AGCCAACCAACCAGTGACC
PRIMER_RIGHT_151_SEQUENCE=AAGCAGCGGTAGGGAATCAC
PRIMER_LEFT_151=460,19
PRIMER_RIGHT_151=552,20
PRIMER_LEFT_151_TM=60.983
PRIMER_RIGHT_151_TM=61.533
PRIMER_LEFT_151_GC_PERCENT=57.895
PRIMER_RIGHT_151_GC_PERCENT=55.000
PRIMER_LEFT_151_SELF_ANY=3.00
PRIMER_RIGHT_151_SELF_ANY=3.00
PRIMER_LEFT_151_SELF_END=1.00
PRIMER_RIGHT_151_SELF_END=3.00
PRIMER_LEFT_151_END_STABILITY=7.9000
PRIMER_RIGHT_151_END_STABILITY=6.3000
PRIMER_PAIR_151_COMPL_ANY=4.00
PRIMER_PAIR_151_COMPL_END=2.00
PRIMER_PAIR_151_PRODUCT_SIZE=93
PRIMER_PAIR_151_PRODUCT_TM=77.3
PRIMER_PAIR_152_PENALTY=3.519008
PRIMER_LEFT_152_PENALTY=1.923751
PRIMER_RIGHT_152_PENALTY=1.595257
PRIMER_LEFT_152_SEQUENCE=CAATGTGGTAGAAGCCAACC
PRIMER_RIGHT_152_SEQUENCE=GTAGGGAATCACAAAGTGGGG
PRIMER_LEFT_152=448,20
PRIMER_RIGHT_152=544,21
PRIMER_LEFT_152_TM=58.076
PRIMER_RIGHT_152_TM=60.595
PRIMER_LEFT_152_GC_PERCENT=50.000
PRIMER_RIGHT_152_GC_PERCENT=52.381
PRIMER_LEFT_152_SELF_ANY=3.00
PRIMER_RIGHT_152_SELF_ANY=3.00
PRIMER_LEFT_152_SELF_END=2.00
PRIMER_RIGHT_152_SELF_END=0.00
PRIMER_LEFT_152_END_STABILITY=8.2000
PRIMER_RIGHT_152_END_STABILITY=11.2000
PRIMER_PAIR_152_COMPL_ANY=4.00
PRIMER_PAIR_152_COMPL_END=2.00
PRIMER_PAIR_152_PRODUCT_SIZE=97
PRIMER_PAIR_152_PRODUCT_TM=76.5
PRIMER_PAIR_153_PENALTY=3.519008
PRIMER_LEFT_153_PENALTY=1.923751
PRIMER_RIGHT_153_PENALTY=1.595257
PRIMER_LEFT_153_SEQUENCE=CCAATGTGGTAGAAGCCAAC
PRIMER_RIGHT_153_SEQUENCE=GGTAGGGAATCACAAAGTGGG
PRIMER_LEFT_153=447,20
PRIMER_RIGHT_153=545,21
PRIMER_LEFT_153_TM=58.076
PRIMER_RIGHT_153_TM=60.595
PRIMER_LEFT_153_GC_PERCENT=50.000
PRIMER_RIGHT_153_GC_PERCENT=52.381
PRIMER_LEFT_153_SELF_ANY=4.00
PRIMER_RIGHT_153_SELF_ANY=3.00
PRIMER_LEFT_153_SELF_END=0.00
PRIMER_RIGHT_153_SELF_END=1.00
PRIMER_LEFT_153_END_STABILITY=8.2000
PRIMER_RIGHT_153_END_STABILITY=9.4000
PRIMER_PAIR_153_COMPL_ANY=4.00
PRIMER_PAIR_153_COMPL_END=3.00
PRIMER_PAIR_153_PRODUCT_SIZE=99
PRIMER_PAIR_153_PRODUCT_TM=77.0
PRIMER_PAIR_154_PENALTY=3.528238
PRIMER_LEFT_154_PENALTY=1.604487
PRIMER_RIGHT_154_PENALTY=1.923751
PRIMER_LEFT_154_SEQUENCE=AGGGACCAAAACCTGCATT
PRIMER_RIGHT_154_SEQUENCE=GGTTGGCTTCTACCACATTG
PRIMER_LEFT_154=370,19
PRIMER_RIGHT_154=467,20
PRIMER_LEFT_154_TM=59.396
PRIMER_RIGHT_154_TM=58.076
PRIMER_LEFT_154_GC_PERCENT=47.368
PRIMER_RIGHT_154_GC_PERCENT=50.000
PRIMER_LEFT_154_SELF_ANY=4.00
PRIMER_RIGHT_154_SELF_ANY=3.00
PRIMER_LEFT_154_SELF_END=1.00
PRIMER_RIGHT_154_SELF_END=3.00
PRIMER_LEFT_154_END_STABILITY=8.4000
PRIMER_RIGHT_154_END_STABILITY=7.2000
PRIMER_PAIR_154_COMPL_ANY=4.00
PRIMER_PAIR_154_COMPL_END=2.00
PRIMER_PAIR_154_PRODUCT_SIZE=98
PRIMER_PAIR_154_PRODUCT_TM=73.9
PRIMER_PAIR_155_PENALTY=3.560259
PRIMER_LEFT_155_PENALTY=3.250857
PRIMER_RIGHT_155_PENALTY=0.309402
PRIMER_LEFT_155_SEQUENCE=GCGCTGGAGGTACCCACT
PRIMER_RIGHT_155_SEQUENCE=CAGTCTGCCACAGAACATGG
PRIMER_LEFT_155=248,18
PRIMER_RIGHT_155=322,20
PRIMER_LEFT_155_TM=61.251
PRIMER_RIGHT_155_TM=60.309
PRIMER_LEFT_155_GC_PERCENT=66.667
PRIMER_RIGHT_155_GC_PERCENT=55.000
PRIMER_LEFT_155_SELF_ANY=6.00
PRIMER_RIGHT_155_SELF_ANY=5.00
PRIMER_LEFT_155_SELF_END=2.00
PRIMER_RIGHT_155_SELF_END=2.00
PRIMER_LEFT_155_END_STABILITY=7.9000
PRIMER_RIGHT_155_END_STABILITY=8.4000
PRIMER_PAIR_155_COMPL_ANY=3.00
PRIMER_PAIR_155_COMPL_END=2.00
PRIMER_PAIR_155_PRODUCT_SIZE=75
PRIMER_PAIR_155_PRODUCT_TM=76.0
PRIMER_PAIR_156_PENALTY=3.568662
PRIMER_LEFT_156_PENALTY=1.902968
PRIMER_RIGHT_156_PENALTY=1.665693
PRIMER_LEFT_156_SEQUENCE=CCCATCCCCACTTTGTGATT
PRIMER_RIGHT_156_SEQUENCE=ACTTGTCAGGAACGAGAAGGG
PRIMER_LEFT_156=519,20
PRIMER_RIGHT_156=596,21
PRIMER_LEFT_156_TM=61.903
PRIMER_RIGHT_156_TM=60.666
PRIMER_LEFT_156_GC_PERCENT=50.000
PRIMER_RIGHT_156_GC_PERCENT=52.381
PRIMER_LEFT_156_SELF_ANY=4.00
PRIMER_RIGHT_156_SELF_ANY=4.00
PRIMER_LEFT_156_SELF_END=2.00
PRIMER_RIGHT_156_SELF_END=0.00
PRIMER_LEFT_156_END_STABILITY=6.9000
PRIMER_RIGHT_156_END_STABILITY=9.7000
PRIMER_PAIR_156_COMPL_ANY=4.00
PRIMER_PAIR_156_COMPL_END=1.00
PRIMER_PAIR_156_PRODUCT_SIZE=78
PRIMER_PAIR_156_PRODUCT_TM=72.7
PRIMER_PAIR_157_PENALTY=3.575421
PRIMER_LEFT_157_PENALTY=1.923751
PRIMER_RIGHT_157_PENALTY=1.651669
PRIMER_LEFT_157_SEQUENCE=ACCAATGTGGTAGAAGCCAA
PRIMER_RIGHT_157_SEQUENCE=AGGGAATCACAAAGTGGGGA
PRIMER_LEFT_157=446,20
PRIMER_RIGHT_157=542,20
PRIMER_LEFT_157_TM=58.076
PRIMER_RIGHT_157_TM=61.652
PRIMER_LEFT_157_GC_PERCENT=45.000
PRIMER_RIGHT_157_GC_PERCENT=50.000
PRIMER_LEFT_157_SELF_ANY=6.00
PRIMER_RIGHT_157_SELF_ANY=3.00
PRIMER_LEFT_157_SELF_END=0.00
PRIMER_RIGHT_157_SELF_END=2.00
PRIMER_LEFT_157_END_STABILITY=10.0000
PRIMER_RIGHT_157_END_STABILITY=10.9000
PRIMER_PAIR_157_COMPL_ANY=4.00
PRIMER_PAIR_157_COMPL_END=0.00
PRIMER_PAIR_157_PRODUCT_SIZE=97
PRIMER_PAIR_157_PRODUCT_TM=76.5
PRIMER_PAIR_158_PENALTY=3.616048
PRIMER_LEFT_158_PENALTY=1.665693
PRIMER_RIGHT_158_PENALTY=1.950354
PRIMER_LEFT_158_SEQUENCE=CCCTTCTCGTTCCTGACAAGT
PRIMER_RIGHT_158_SEQUENCE=CTGCATGTCTCTTTGGCGAC
PRIMER_LEFT_158=576,21
PRIMER_RIGHT_158=675,20
PRIMER_LEFT_158_TM=60.666
PRIMER_RIGHT_158_TM=61.950
PRIMER_LEFT_158_GC_PERCENT=52.381
PRIMER_RIGHT_158_GC_PERCENT=55.000
PRIMER_LEFT_158_SELF_ANY=4.00
PRIMER_RIGHT_158_SELF_ANY=4.00
PRIMER_LEFT_158_SELF_END=2.00
PRIMER_RIGHT_158_SELF_END=2.00
PRIMER_LEFT_158_END_STABILITY=6.7000
PRIMER_RIGHT_158_END_STABILITY=9.6000
PRIMER_PAIR_158_COMPL_ANY=4.00
PRIMER_PAIR_158_COMPL_END=2.00
PRIMER_PAIR_158_PRODUCT_SIZE=100
PRIMER_PAIR_158_PRODUCT_TM=74.4
PRIMER_PAIR_159_PENALTY=3.629273
PRIMER_LEFT_159_PENALTY=2.182723
PRIMER_RIGHT_159_PENALTY=1.446550
PRIMER_LEFT_159_SEQUENCE=ATCCAGAACTGGTGCAAGC
PRIMER_RIGHT_159_SEQUENCE=CTCACCAACTAAGCAGCGGTA
PRIMER_LEFT_159=479,19
PRIMER_RIGHT_159=562,21
PRIMER_LEFT_159_TM=58.817
PRIMER_RIGHT_159_TM=60.447
PRIMER_LEFT_159_GC_PERCENT=52.632
PRIMER_RIGHT_159_GC_PERCENT=52.381
PRIMER_LEFT_159_SELF_ANY=6.00
PRIMER_RIGHT_159_SELF_ANY=3.00
PRIMER_LEFT_159_SELF_END=2.00
PRIMER_RIGHT_159_SELF_END=2.00
PRIMER_LEFT_159_END_STABILITY=8.5000
PRIMER_RIGHT_159_END_STABILITY=8.9000
PRIMER_PAIR_159_COMPL_ANY=5.00
PRIMER_PAIR_159_COMPL_END=1.00
PRIMER_PAIR_159_PRODUCT_SIZE=84
PRIMER_PAIR_159_PRODUCT_TM=76.2
PRIMER_PAIR_160_PENALTY=3.629920
PRIMER_LEFT_160_PENALTY=1.701257
PRIMER_RIGHT_160_PENALTY=1.928663
PRIMER_LEFT_160_SEQUENCE=ATTCCCTACCGCTGCTTAGTT
PRIMER_RIGHT_160_SEQUENCE=CAAACATCCATCCTCTCCTG
PRIMER_LEFT_160=536,21
PRIMER_RIGHT_160=630,20
PRIMER_LEFT_160_TM=59.299
PRIMER_RIGHT_160_TM=58.071
PRIMER_LEFT_160_GC_PERCENT=47.619
PRIMER_RIGHT_160_GC_PERCENT=50.000
PRIMER_LEFT_160_SELF_ANY=3.00
PRIMER_RIGHT_160_SELF_ANY=2.00
PRIMER_LEFT_160_SELF_END=0.00
PRIMER_RIGHT_160_SELF_END=1.00
PRIMER_LEFT_160_END_STABILITY=5.7000
PRIMER_RIGHT_160_END_STABILITY=8.2000
PRIMER_PAIR_160_COMPL_ANY=3.00
PRIMER_PAIR_160_COMPL_END=0.00
PRIMER_PAIR_160_PRODUCT_SIZE=95
PRIMER_PAIR_160_PRODUCT_TM=72.6
PRIMER_PAIR_161_PENALTY=3.633949
PRIMER_LEFT_161_PENALTY=1.683594
PRIMER_RIGHT_161_PENALTY=1.950354
PRIMER_LEFT_161_SEQUENCE=TCGTTCCTGACAAGTGCAAAT
PRIMER_RIGHT_161_SEQUENCE=ACTGCATGTCTCTTTGGCGA
PRIMER_LEFT_161=582,21
PRIMER_RIGHT_161=676,20
PRIMER_LEFT_161_TM=60.684
PRIMER_RIGHT_161_TM=61.950
PRIMER_LEFT_161_GC_PERCENT=42.857
PRIMER_RIGHT_161_GC_PERCENT=50.000
PRIMER_LEFT_161_SELF_ANY=4.00
PRIMER_RIGHT_161_SELF_ANY=4.00
PRIMER_LEFT_161_SELF_END=2.00
PRIMER_RIGHT_161_SELF_END=2.00
PRIMER_LEFT_161_END_STABILITY=7.2000
PRIMER_RIGHT_161_END_STABILITY=11.4000
PRIMER_PAIR_161_COMPL_ANY=4.00
PRIMER_PAIR_161_COMPL_END=1.00
PRIMER_PAIR_161_PRODUCT_SIZE=95
PRIMER_PAIR_161_PRODUCT_TM=73.4
PRIMER_PAIR_162_PENALTY=3.637509
PRIMER_LEFT_162_PENALTY=1.928458
PRIMER_RIGHT_162_PENALTY=1.709051
PRIMER_LEFT_162_SEQUENCE=CACCAATGTGGTAGAAGCCAA
PRIMER_RIGHT_162_SEQUENCE=GGGAATCACAAAGTGGGGA
PRIMER_LEFT_162=445,21
PRIMER_RIGHT_162=541,19
PRIMER_LEFT_162_TM=60.928
PRIMER_RIGHT_162_TM=60.709
PRIMER_LEFT_162_GC_PERCENT=47.619
PRIMER_RIGHT_162_GC_PERCENT=52.632
PRIMER_LEFT_162_SELF_ANY=6.00
PRIMER_RIGHT_162_SELF_ANY=3.00
PRIMER_LEFT_162_SELF_END=0.00
PRIMER_RIGHT_162_SELF_END=2.00
PRIMER_LEFT_162_END_STABILITY=10.0000
PRIMER_RIGHT_162_END_STABILITY=10.9000
PRIMER_PAIR_162_COMPL_ANY=4.00
PRIMER_PAIR_162_COMPL_END=0.00
PRIMER_PAIR_162_PRODUCT_SIZE=97
PRIMER_PAIR_162_PRODUCT_TM=77.0
PRIMER_PAIR_163_PENALTY=3.651147
PRIMER_LEFT_163_PENALTY=2.275406
PRIMER_RIGHT_163_PENALTY=1.375741
PRIMER_LEFT_163_SEQUENCE=ACACCGTCGCCAAAGAGAC
PRIMER_RIGHT_163_SEQUENCE=AACTCTACCCCTCGGAACTTG
PRIMER_LEFT_163=651,19
PRIMER_RIGHT_163=750,21
PRIMER_LEFT_163_TM=61.275
PRIMER_RIGHT_163_TM=59.624
PRIMER_LEFT_163_GC_PERCENT=57.895
PRIMER_RIGHT_163_GC_PERCENT=52.381
PRIMER_LEFT_163_SELF_ANY=3.00
PRIMER_RIGHT_163_SELF_ANY=3.00
PRIMER_LEFT_163_SELF_END=2.00
PRIMER_RIGHT_163_SELF_END=1.00
PRIMER_LEFT_163_END_STABILITY=6.1000
PRIMER_RIGHT_163_END_STABILITY=6.7000
PRIMER_PAIR_163_COMPL_ANY=4.00
PRIMER_PAIR_163_COMPL_END=1.00
PRIMER_PAIR_163_PRODUCT_SIZE=100
PRIMER_PAIR_163_PRODUCT_TM=75.2
PRIMER_PAIR_164_PENALTY=3.652405
PRIMER_LEFT_164_PENALTY=1.557214
PRIMER_RIGHT_164_PENALTY=2.095192
PRIMER_LEFT_164_SEQUENCE=CGAAACTCATCTTCACTGGC
PRIMER_RIGHT_164_SEQUENCE=CATGCCGTAGTCATGCAAGTT
PRIMER_LEFT_164=631,20
PRIMER_RIGHT_164=709,21
PRIMER_LEFT_164_TM=58.443
PRIMER_RIGHT_164_TM=61.095
PRIMER_LEFT_164_GC_PERCENT=50.000
PRIMER_RIGHT_164_GC_PERCENT=47.619
PRIMER_LEFT_164_SELF_ANY=3.00
PRIMER_RIGHT_164_SELF_ANY=6.00
PRIMER_LEFT_164_SELF_END=2.00
PRIMER_RIGHT_164_SELF_END=3.00
PRIMER_LEFT_164_END_STABILITY=9.7000
PRIMER_RIGHT_164_END_STABILITY=6.7000
PRIMER_PAIR_164_COMPL_ANY=4.00
PRIMER_PAIR_164_COMPL_END=3.00
PRIMER_PAIR_164_PRODUCT_SIZE=79
PRIMER_PAIR_164_PRODUCT_TM=73.1
PRIMER_PAIR_165_PENALTY=3.656409
PRIMER_LEFT_165_PENALTY=1.846031
PRIMER_RIGHT_165_PENALTY=1.810378
PRIMER_LEFT_165_SEQUENCE=GAACTGCAGATCACCAATGTG
PRIMER_RIGHT_165_SEQUENCE=GATGGGTCTTGCACTGCTTG
PRIMER_LEFT_165=434,21
PRIMER_RIGHT_165=524,20
PRIMER_LEFT_165_TM=59.154
PRIMER_RIGHT_165_TM=61.810
PRIMER_LEFT_165_GC_PERCENT=47.619
PRIMER_RIGHT_165_GC_PERCENT=55.000
PRIMER_LEFT_165_SELF_ANY=8.00
PRIMER_RIGHT_165_SELF_ANY=5.00
PRIMER_LEFT_165_SELF_END=3.00
PRIMER_RIGHT_165_SELF_END=3.00
PRIMER_LEFT_165_END_STABILITY=6.6000
PRIMER_RIGHT_165_END_STABILITY=8.5000
PRIMER_PAIR_165_COMPL_ANY=4.00
PRIMER_PAIR_165_COMPL_END=1.00
PRIMER_PAIR_165_PRODUCT_SIZE=91
PRIMER_PAIR_165_PRODUCT_TM=76.3
PRIMER_PAIR_166_PENALTY=3.661578
PRIMER_LEFT_166_PENALTY=1.595257
PRIMER_RIGHT_166_PENALTY=2.066321
PRIMER_LEFT_166_SEQUENCE=CCCACTTTGTGATTCCCTACC
PRIMER_RIGHT_166_SEQUENCE=TTGCACTTGTCAGGAACGA
PRIMER_LEFT_166=525,21
PRIMER_RIGHT_166=600,19
PRIMER_LEFT_166_TM=60.595
PRIMER_RIGHT_166_TM=58.934
PRIMER_LEFT_166_GC_PERCENT=52.381
PRIMER_RIGHT_166_GC_PERCENT=47.368
PRIMER_LEFT_166_SELF_ANY=3.00
PRIMER_RIGHT_166_SELF_ANY=4.00
PRIMER_LEFT_166_SELF_END=0.00
PRIMER_RIGHT_166_SELF_END=1.00
PRIMER_LEFT_166_END_STABILITY=6.9000
PRIMER_RIGHT_166_END_STABILITY=8.4000
PRIMER_PAIR_166_COMPL_ANY=4.00
PRIMER_PAIR_166_COMPL_END=2.00
PRIMER_PAIR_166_PRODUCT_SIZE=76
PRIMER_PAIR_166_PRODUCT_TM=72.0
PRIMER_PAIR_167_PENALTY=3.680580
PRIMER_LEFT_167_PENALTY=2.137489
PRIMER_RIGHT_167_PENALTY=1.543091
PRIMER_LEFT_167_SEQUENCE=GGTACCCACTGATGGTAATGCT
PRIMER_RIGHT_167_SEQUENCE=CCACTTCCCATTCTGGACA
PRIMER_LEFT_167=256,22
PRIMER_RIGHT_167=355,19
PRIMER_LEFT_167_TM=60.137
PRIMER_RIGHT_167_TM=59.457
PRIMER_LEFT_167_GC_PERCENT=50.000
PRIMER_RIGHT_167_GC_PERCENT=52.632
PRIMER_LEFT_167_SELF_ANY=6.00
PRIMER_RIGHT_167_SELF_ANY=3.00
PRIMER_LEFT_167_SELF_END=0.00
PRIMER_RIGHT_167_SELF_END=2.00
PRIMER_LEFT_167_END_STABILITY=8.1000
PRIMER_RIGHT_167_END_STABILITY=7.9000
PRIMER_PAIR_167_COMPL_ANY=5.00
PRIMER_PAIR_167_COMPL_END=2.00
PRIMER_PAIR_167_PRODUCT_SIZE=100
PRIMER_PAIR_167_PRODUCT_TM=75.6
PRIMER_PAIR_168_PENALTY=3.683230
PRIMER_LEFT_168_PENALTY=1.812888
PRIMER_RIGHT_168_PENALTY=1.870342
PRIMER_LEFT_168_SEQUENCE=CGCTGCTTAGTTGGTGAGTT
PRIMER_RIGHT_168_SEQUENCE=AGTTTCGCAAACATCCATCCT
PRIMER_LEFT_168=545,20
PRIMER_RIGHT_168=637,21
PRIMER_LEFT_168_TM=58.187
PRIMER_RIGHT_168_TM=60.870
PRIMER_LEFT_168_GC_PERCENT=50.000
PRIMER_RIGHT_168_GC_PERCENT=42.857
PRIMER_LEFT_168_SELF_ANY=3.00
PRIMER_RIGHT_168_SELF_ANY=6.00
PRIMER_LEFT_168_SELF_END=0.00
PRIMER_RIGHT_168_SELF_END=0.00
PRIMER_LEFT_168_END_STABILITY=6.4000
PRIMER_RIGHT_168_END_STABILITY=7.8000
PRIMER_PAIR_168_COMPL_ANY=4.00
PRIMER_PAIR_168_COMPL_END=1.00
PRIMER_PAIR_168_PRODUCT_SIZE=93
PRIMER_PAIR_168_PRODUCT_TM=72.4
PRIMER_PAIR_169_PENALTY=3.734075
PRIMER_LEFT_169_PENALTY=1.566355
PRIMER_RIGHT_169_PENALTY=2.167720
PRIMER_LEFT_169_SEQUENCE=ATGCCCTTCTCGTTCCTGAC
PRIMER_RIGHT_169_SEQUENCE=ACGGTGTGCCAGTGAAGATG
PRIMER_LEFT_169=573,20
PRIMER_RIGHT_169=657,20
PRIMER_LEFT_169_TM=61.566
PRIMER_RIGHT_169_TM=62.168
PRIMER_LEFT_169_GC_PERCENT=55.000
PRIMER_RIGHT_169_GC_PERCENT=55.000
PRIMER_LEFT_169_SELF_ANY=2.00
PRIMER_RIGHT_169_SELF_ANY=3.00
PRIMER_LEFT_169_SELF_END=1.00
PRIMER_RIGHT_169_SELF_END=0.00
PRIMER_LEFT_169_END_STABILITY=6.4000
PRIMER_RIGHT_169_END_STABILITY=6.6000
PRIMER_PAIR_169_COMPL_ANY=4.00
PRIMER_PAIR_169_COMPL_END=3.00
PRIMER_PAIR_169_PRODUCT_SIZE=85
PRIMER_PAIR_169_PRODUCT_TM=72.6
PRIMER_PAIR_170_PENALTY=3.741350
PRIMER_LEFT_170_PENALTY=0.663548
PRIMER_RIGHT_170_PENALTY=3.077802
PRIMER_LEFT_170_SEQUENCE=CAGAATGGGAAGTGGGATTC
PRIMER_RIGHT_170_SEQUENCE=GTTCAGGGTAGACTTCTTGGC
PRIMER_LEFT_170=341,20
PRIMER_RIGHT_170=437,21
PRIMER_LEFT_170_TM=59.336
PRIMER_RIGHT_170_TM=57.922
PRIMER_LEFT_170_GC_PERCENT=50.000
PRIMER_RIGHT_170_GC_PERCENT=52.381
PRIMER_LEFT_170_SELF_ANY=4.00
PRIMER_RIGHT_170_SELF_ANY=4.00
PRIMER_LEFT_170_SELF_END=3.00
PRIMER_RIGHT_170_SELF_END=2.00
PRIMER_LEFT_170_END_STABILITY=6.6000
PRIMER_RIGHT_170_END_STABILITY=10.0000
PRIMER_PAIR_170_COMPL_ANY=5.00
PRIMER_PAIR_170_COMPL_END=0.00
PRIMER_PAIR_170_PRODUCT_SIZE=97
PRIMER_PAIR_170_PRODUCT_TM=73.6
PRIMER_PAIR_171_PENALTY=3.741976
PRIMER_LEFT_171_PENALTY=2.137489
PRIMER_RIGHT_171_PENALTY=1.604487
PRIMER_LEFT_171_SEQUENCE=AGGTACCCACTGATGGTAATGC
PRIMER_RIGHT_171_SEQUENCE=ACTTCCCATTCTGGACATTCA
PRIMER_LEFT_171=255,22
PRIMER_RIGHT_171=353,21
PRIMER_LEFT_171_TM=60.137
PRIMER_RIGHT_171_TM=59.396
PRIMER_LEFT_171_GC_PERCENT=50.000
PRIMER_RIGHT_171_GC_PERCENT=42.857
PRIMER_LEFT_171_SELF_ANY=6.00
PRIMER_RIGHT_171_SELF_ANY=3.00
PRIMER_LEFT_171_SELF_END=2.00
PRIMER_RIGHT_171_SELF_END=2.00
PRIMER_LEFT_171_END_STABILITY=8.4000
PRIMER_RIGHT_171_END_STABILITY=6.9000
PRIMER_PAIR_171_COMPL_ANY=5.00
PRIMER_PAIR_171_COMPL_END=2.00
PRIMER_PAIR_171_PRODUCT_SIZE=99
PRIMER_PAIR_171_PRODUCT_TM=75.0
PRIMER_PAIR_172_PENALTY=3.751205
PRIMER_LEFT_172_PENALTY=2.563176
PRIMER_RIGHT_172_PENALTY=1.188029
PRIMER_LEFT_172_SEQUENCE=GTTTGGCACTGCTCCTGCT
PRIMER_RIGHT_172_SEQUENCE=AACATGGCAATCTGGGGTT
PRIMER_LEFT_172=210,19
PRIMER_RIGHT_172=309,19
PRIMER_LEFT_172_TM=61.563
PRIMER_RIGHT_172_TM=60.188
PRIMER_LEFT_172_GC_PERCENT=57.895
PRIMER_RIGHT_172_GC_PERCENT=47.368
PRIMER_LEFT_172_SELF_ANY=5.00
PRIMER_RIGHT_172_SELF_ANY=4.00
PRIMER_LEFT_172_SELF_END=0.00
PRIMER_RIGHT_172_SELF_END=1.00
PRIMER_LEFT_172_END_STABILITY=8.2000
PRIMER_RIGHT_172_END_STABILITY=9.4000
PRIMER_PAIR_172_COMPL_ANY=4.00
PRIMER_PAIR_172_COMPL_END=0.00
PRIMER_PAIR_172_PRODUCT_SIZE=100
PRIMER_PAIR_172_PRODUCT_TM=79.7
PRIMER_PAIR_173_PENALTY=3.754500
PRIMER_LEFT_173_PENALTY=2.150273
PRIMER_RIGHT_173_PENALTY=1.604227
PRIMER_LEFT_173_SEQUENCE=CCCCAGATTGCCATGTTC
PRIMER_RIGHT_173_SEQUENCE=CCCTGATGGATCTGAATCCC
PRIMER_LEFT_173=293,18
PRIMER_RIGHT_173=373,20
PRIMER_LEFT_173_TM=59.850
PRIMER_RIGHT_173_TM=61.604
PRIMER_LEFT_173_GC_PERCENT=55.556
PRIMER_RIGHT_173_GC_PERCENT=55.000
PRIMER_LEFT_173_SELF_ANY=4.00
PRIMER_RIGHT_173_SELF_ANY=5.00
PRIMER_LEFT_173_SELF_END=0.00
PRIMER_RIGHT_173_SELF_END=3.00
PRIMER_LEFT_173_END_STABILITY=6.7000
PRIMER_RIGHT_173_END_STABILITY=9.3000
PRIMER_PAIR_173_COMPL_ANY=5.00
PRIMER_PAIR_173_COMPL_END=0.00
PRIMER_PAIR_173_PRODUCT_SIZE=81
PRIMER_PAIR_173_PRODUCT_TM=73.2
PRIMER_PAIR_174_PENALTY=3.758018
PRIMER_LEFT_174_PENALTY=2.402111
PRIMER_RIGHT_174_PENALTY=1.355907
PRIMER_LEFT_174_SEQUENCE=ACCGTCGCCAAAGAGACA
PRIMER_RIGHT_174_SEQUENCE=CAAACTCTACCCCTCGGAAC
PRIMER_LEFT_174=653,18
PRIMER_RIGHT_174=752,20
PRIMER_LEFT_174_TM=60.402
PRIMER_RIGHT_174_TM=58.644
PRIMER_LEFT_174_GC_PERCENT=55.556
PRIMER_RIGHT_174_GC_PERCENT=55.000
PRIMER_LEFT_174_SELF_ANY=3.00
PRIMER_RIGHT_174_SELF_ANY=3.00
PRIMER_LEFT_174_SELF_END=0.00
PRIMER_RIGHT_174_SELF_END=0.00
PRIMER_LEFT_174_END_STABILITY=6.4000
PRIMER_RIGHT_174_END_STABILITY=7.9000
PRIMER_PAIR_174_COMPL_ANY=4.00
PRIMER_PAIR_174_COMPL_END=0.00
PRIMER_PAIR_174_PRODUCT_SIZE=100
PRIMER_PAIR_174_PRODUCT_TM=75.2
PRIMER_PAIR_175_PENALTY=3.764855
PRIMER_LEFT_175_PENALTY=2.063598
PRIMER_RIGHT_175_PENALTY=1.701257
PRIMER_LEFT_175_SEQUENCE=GCCAACCAACCAGTGACC
PRIMER_RIGHT_175_SEQUENCE=AACTAAGCAGCGGTAGGGAAT
PRIMER_LEFT_175=461,18
PRIMER_RIGHT_175=556,21
PRIMER_LEFT_175_TM=59.936
PRIMER_RIGHT_175_TM=59.299
PRIMER_LEFT_175_GC_PERCENT=61.111
PRIMER_RIGHT_175_GC_PERCENT=47.619
PRIMER_LEFT_175_SELF_ANY=3.00
PRIMER_RIGHT_175_SELF_ANY=3.00
PRIMER_LEFT_175_SELF_END=1.00
PRIMER_RIGHT_175_SELF_END=2.00
PRIMER_LEFT_175_END_STABILITY=7.9000
PRIMER_RIGHT_175_END_STABILITY=8.1000
PRIMER_PAIR_175_COMPL_ANY=4.00
PRIMER_PAIR_175_COMPL_END=0.00
PRIMER_PAIR_175_PRODUCT_SIZE=96
PRIMER_PAIR_175_PRODUCT_TM=77.1
PRIMER_PAIR_176_PENALTY=3.771832
PRIMER_LEFT_176_PENALTY=1.749643
PRIMER_RIGHT_176_PENALTY=2.022189
PRIMER_LEFT_176_SEQUENCE=TGATTCCCTACCGCTGCTT
PRIMER_RIGHT_176_SEQUENCE=CATCCATCCTCTCCTGGTG
PRIMER_LEFT_176=534,19
PRIMER_RIGHT_176=626,19
PRIMER_LEFT_176_TM=60.750
PRIMER_RIGHT_176_TM=58.978
PRIMER_LEFT_176_GC_PERCENT=52.632
PRIMER_RIGHT_176_GC_PERCENT=57.895
PRIMER_LEFT_176_SELF_ANY=3.00
PRIMER_RIGHT_176_SELF_ANY=3.00
PRIMER_LEFT_176_SELF_END=0.00
PRIMER_RIGHT_176_SELF_END=3.00
PRIMER_LEFT_176_END_STABILITY=8.5000
PRIMER_RIGHT_176_END_STABILITY=8.2000
PRIMER_PAIR_176_COMPL_ANY=3.00
PRIMER_PAIR_176_COMPL_END=0.00
PRIMER_PAIR_176_PRODUCT_SIZE=93
PRIMER_PAIR_176_PRODUCT_TM=72.8
PRIMER_PAIR_177_PENALTY=3.787393
PRIMER_LEFT_177_PENALTY=2.431486
PRIMER_RIGHT_177_PENALTY=1.355907
PRIMER_LEFT_177_SEQUENCE=CCAAAGAGACATGCAGTGAGAA
PRIMER_RIGHT_177_SEQUENCE=ACAAACTCTACCCCTCGGAA
PRIMER_LEFT_177=660,22
PRIMER_RIGHT_177=753,20
PRIMER_LEFT_177_TM=60.431
PRIMER_RIGHT_177_TM=58.644
PRIMER_LEFT_177_GC_PERCENT=45.455
PRIMER_RIGHT_177_GC_PERCENT=50.000
PRIMER_LEFT_177_SELF_ANY=4.00
PRIMER_RIGHT_177_SELF_ANY=3.00
PRIMER_LEFT_177_SELF_END=0.00
PRIMER_RIGHT_177_SELF_END=1.00
PRIMER_LEFT_177_END_STABILITY=6.7000
PRIMER_RIGHT_177_END_STABILITY=10.2000
PRIMER_PAIR_177_COMPL_ANY=4.00
PRIMER_PAIR_177_COMPL_END=0.00
PRIMER_PAIR_177_PRODUCT_SIZE=94
PRIMER_PAIR_177_PRODUCT_TM=74.0
PRIMER_PAIR_178_PENALTY=3.794945
PRIMER_LEFT_178_PENALTY=2.485003
PRIMER_RIGHT_178_PENALTY=1.309943
PRIMER_LEFT_178_SEQUENCE=TACCCACTGATGGTAATGCTG
PRIMER_RIGHT_178_SEQUENCE=CCCACTTCCCATTCTGGAC
PRIMER_LEFT_178=258,21
PRIMER_RIGHT_178=356,19
PRIMER_LEFT_178_TM=58.515
PRIMER_RIGHT_178_TM=60.310
PRIMER_LEFT_178_GC_PERCENT=47.619
PRIMER_RIGHT_178_GC_PERCENT=57.895
PRIMER_LEFT_178_SELF_ANY=5.00
PRIMER_RIGHT_178_SELF_ANY=3.00
PRIMER_LEFT_178_SELF_END=1.00
PRIMER_RIGHT_178_SELF_END=1.00
PRIMER_LEFT_178_END_STABILITY=8.5000
PRIMER_RIGHT_178_END_STABILITY=7.9000
PRIMER_PAIR_178_COMPL_ANY=5.00
PRIMER_PAIR_178_COMPL_END=1.00
PRIMER_PAIR_178_PRODUCT_SIZE=99
PRIMER_PAIR_178_PRODUCT_TM=75.4
PRIMER_PAIR_179_PENALTY=3.798463
PRIMER_LEFT_179_PENALTY=2.045801
PRIMER_RIGHT_179_PENALTY=1.752662
PRIMER_LEFT_179_SEQUENCE=CAGTGCAAGACCCATCCC
PRIMER_RIGHT_179_SEQUENCE=AGGAACGAGAAGGGCATCA
PRIMER_LEFT_179=509,18
PRIMER_RIGHT_179=589,19
PRIMER_LEFT_179_TM=60.046
PRIMER_RIGHT_179_TM=60.753
PRIMER_LEFT_179_GC_PERCENT=61.111
PRIMER_RIGHT_179_GC_PERCENT=52.632
PRIMER_LEFT_179_SELF_ANY=4.00
PRIMER_RIGHT_179_SELF_ANY=2.00
PRIMER_LEFT_179_SELF_END=0.00
PRIMER_RIGHT_179_SELF_END=1.00
PRIMER_LEFT_179_END_STABILITY=9.3000
PRIMER_RIGHT_179_END_STABILITY=6.9000
PRIMER_PAIR_179_COMPL_ANY=4.00
PRIMER_PAIR_179_COMPL_END=3.00
PRIMER_PAIR_179_PRODUCT_SIZE=81
PRIMER_PAIR_179_PRODUCT_TM=73.2
PRIMER_PAIR_180_PENALTY=3.810679
PRIMER_LEFT_180_PENALTY=2.027792
PRIMER_RIGHT_180_PENALTY=1.782887
PRIMER_LEFT_180_SEQUENCE=ATGTGGTAGAAGCCAACCAAC
PRIMER_RIGHT_180_SEQUENCE=ACAAAGTGGGGATGGGTCT
PRIMER_LEFT_180=450,21
PRIMER_RIGHT_180=534,19
PRIMER_LEFT_180_TM=58.972
PRIMER_RIGHT_180_TM=59.217
PRIMER_LEFT_180_GC_PERCENT=47.619
PRIMER_RIGHT_180_GC_PERCENT=52.632
PRIMER_LEFT_180_SELF_ANY=4.00
PRIMER_RIGHT_180_SELF_ANY=2.00
PRIMER_LEFT_180_SELF_END=2.00
PRIMER_RIGHT_180_SELF_END=0.00
PRIMER_LEFT_180_END_STABILITY=8.2000
PRIMER_RIGHT_180_END_STABILITY=7.6000
PRIMER_PAIR_180_COMPL_ANY=4.00
PRIMER_PAIR_180_COMPL_END=3.00
PRIMER_PAIR_180_PRODUCT_SIZE=85
PRIMER_PAIR_180_PRODUCT_TM=76.0
PRIMER_PAIR_181_PENALTY=3.813241
PRIMER_LEFT_181_PENALTY=2.246886
PRIMER_RIGHT_181_PENALTY=1.566355
PRIMER_LEFT_181_SEQUENCE=AGCAGTGCAAGACCCATCC
PRIMER_RIGHT_181_SEQUENCE=AGGAACGAGAAGGGCATCAC
PRIMER_LEFT_181=507,19
PRIMER_RIGHT_181=589,20
PRIMER_LEFT_181_TM=61.247
PRIMER_RIGHT_181_TM=61.566
PRIMER_LEFT_181_GC_PERCENT=57.895
PRIMER_RIGHT_181_GC_PERCENT=55.000
PRIMER_LEFT_181_SELF_ANY=5.00
PRIMER_RIGHT_181_SELF_ANY=2.00
PRIMER_LEFT_181_SELF_END=0.00
PRIMER_RIGHT_181_SELF_END=2.00
PRIMER_LEFT_181_END_STABILITY=8.1000
PRIMER_RIGHT_181_END_STABILITY=6.3000
PRIMER_PAIR_181_COMPL_ANY=4.00
PRIMER_PAIR_181_COMPL_END=3.00
PRIMER_PAIR_181_PRODUCT_SIZE=83
PRIMER_PAIR_181_PRODUCT_TM=73.4
PRIMER_PAIR_182_PENALTY=3.830330
PRIMER_LEFT_182_PENALTY=1.852336
PRIMER_RIGHT_182_PENALTY=1.977994
PRIMER_LEFT_182_SEQUENCE=TCTTACACCAGGAGAGGATGG
PRIMER_RIGHT_182_SEQUENCE=TCTCACTGCATGTCTCTTTGG
PRIMER_LEFT_182=603,21
PRIMER_RIGHT_182=680,21
PRIMER_LEFT_182_TM=59.148
PRIMER_RIGHT_182_TM=59.022
PRIMER_LEFT_182_GC_PERCENT=52.381
PRIMER_RIGHT_182_GC_PERCENT=47.619
PRIMER_LEFT_182_SELF_ANY=3.00
PRIMER_RIGHT_182_SELF_ANY=4.00
PRIMER_LEFT_182_SELF_END=0.00
PRIMER_RIGHT_182_SELF_END=0.00
PRIMER_LEFT_182_END_STABILITY=8.1000
PRIMER_RIGHT_182_END_STABILITY=8.8000
PRIMER_PAIR_182_COMPL_ANY=5.00
PRIMER_PAIR_182_COMPL_END=3.00
PRIMER_PAIR_182_PRODUCT_SIZE=78
PRIMER_PAIR_182_PRODUCT_TM=72.7
PRIMER_PAIR_183_PENALTY=3.831476
PRIMER_LEFT_183_PENALTY=1.907724
PRIMER_RIGHT_183_PENALTY=1.923751
PRIMER_LEFT_183_SEQUENCE=CAGGGACCAAAACCTGCAT
PRIMER_RIGHT_183_SEQUENCE=TGGTTGGCTTCTACCACATT
PRIMER_LEFT_183=369,19
PRIMER_RIGHT_183=468,20
PRIMER_LEFT_183_TM=60.908
PRIMER_RIGHT_183_TM=58.076
PRIMER_LEFT_183_GC_PERCENT=52.632
PRIMER_RIGHT_183_GC_PERCENT=45.000
PRIMER_LEFT_183_SELF_ANY=4.00
PRIMER_RIGHT_183_SELF_ANY=4.00
PRIMER_LEFT_183_SELF_END=2.00
PRIMER_RIGHT_183_SELF_END=1.00
PRIMER_LEFT_183_END_STABILITY=8.4000
PRIMER_RIGHT_183_END_STABILITY=6.6000
PRIMER_PAIR_183_COMPL_ANY=4.00
PRIMER_PAIR_183_COMPL_END=1.00
PRIMER_PAIR_183_PRODUCT_SIZE=100
PRIMER_PAIR_183_PRODUCT_TM=74.0
PRIMER_PAIR_184_PENALTY=3.834074
PRIMER_LEFT_184_PENALTY=2.203644
PRIMER_RIGHT_184_PENALTY=1.630431
PRIMER_LEFT_184_SEQUENCE=CTGGAGGTACCCACTGATGGT
PRIMER_RIGHT_184_SEQUENCE=GTGCATGTTCAGTCTGCCA
PRIMER_LEFT_184=251,21
PRIMER_RIGHT_184=331,19
PRIMER_LEFT_184_TM=61.204
PRIMER_RIGHT_184_TM=59.370
PRIMER_LEFT_184_GC_PERCENT=57.143
PRIMER_RIGHT_184_GC_PERCENT=52.632
PRIMER_LEFT_184_SELF_ANY=6.00
PRIMER_RIGHT_184_SELF_ANY=5.00
PRIMER_LEFT_184_SELF_END=3.00
PRIMER_RIGHT_184_SELF_END=3.00
PRIMER_LEFT_184_END_STABILITY=7.8000
PRIMER_RIGHT_184_END_STABILITY=10.0000
PRIMER_PAIR_184_COMPL_ANY=5.00
PRIMER_PAIR_184_COMPL_END=2.00
PRIMER_PAIR_184_PRODUCT_SIZE=81
PRIMER_PAIR_184_PRODUCT_TM=75.3
PRIMER_PAIR_185_PENALTY=3.841761
PRIMER_LEFT_185_PENALTY=1.566355
PRIMER_RIGHT_185_PENALTY=2.275406
PRIMER_LEFT_185_SEQUENCE=GTGATGCCCTTCTCGTTCCT
PRIMER_RIGHT_185_SEQUENCE=GTCTCTTTGGCGACGGTGT
PRIMER_LEFT_185=570,20
PRIMER_RIGHT_185=669,19
PRIMER_LEFT_185_TM=61.566
PRIMER_RIGHT_185_TM=61.275
PRIMER_LEFT_185_GC_PERCENT=55.000
PRIMER_RIGHT_185_GC_PERCENT=57.895
PRIMER_LEFT_185_SELF_ANY=2.00
PRIMER_RIGHT_185_SELF_ANY=3.00
PRIMER_LEFT_185_SELF_END=0.00
PRIMER_RIGHT_185_SELF_END=1.00
PRIMER_LEFT_185_END_STABILITY=8.2000
PRIMER_RIGHT_185_END_STABILITY=7.6000
PRIMER_PAIR_185_COMPL_ANY=3.00
PRIMER_PAIR_185_COMPL_END=0.00
PRIMER_PAIR_185_PRODUCT_SIZE=100
PRIMER_PAIR_185_PRODUCT_TM=74.4
PRIMER_PAIR_186_PENALTY=3.843348
PRIMER_LEFT_186_PENALTY=1.852336
PRIMER_RIGHT_186_PENALTY=1.991012
PRIMER_LEFT_186_SEQUENCE=CTTACACCAGGAGAGGATGGA
PRIMER_RIGHT_186_SEQUENCE=CTCACTGCATGTCTCTTTGGC
PRIMER_LEFT_186=604,21
PRIMER_RIGHT_186=679,21
PRIMER_LEFT_186_TM=59.148
PRIMER_RIGHT_186_TM=60.991
PRIMER_LEFT_186_GC_PERCENT=52.381
PRIMER_RIGHT_186_GC_PERCENT=52.381
PRIMER_LEFT_186_SELF_ANY=3.00
PRIMER_RIGHT_186_SELF_ANY=4.00
PRIMER_LEFT_186_SELF_END=0.00
PRIMER_RIGHT_186_SELF_END=2.00
PRIMER_LEFT_186_END_STABILITY=8.1000
PRIMER_RIGHT_186_END_STABILITY=10.0000
PRIMER_PAIR_186_COMPL_ANY=4.00
PRIMER_PAIR_186_COMPL_END=3.00
PRIMER_PAIR_186_PRODUCT_SIZE=76
PRIMER_PAIR_186_PRODUCT_TM=73.0
PRIMER_PAIR_187_PENALTY=3.847029
PRIMER_LEFT_187_PENALTY=2.631568
PRIMER_RIGHT_187_PENALTY=1.215461
PRIMER_LEFT_187_SEQUENCE=ATCCTGCAGTATTGCCAAGAAG
PRIMER_RIGHT_187_SEQUENCE=TGGATGGTCACTGGTTGGT
PRIMER_LEFT_187=404,22
PRIMER_RIGHT_187=483,19
PRIMER_LEFT_187_TM=60.632
PRIMER_RIGHT_187_TM=59.785
PRIMER_LEFT_187_GC_PERCENT=45.455
PRIMER_RIGHT_187_GC_PERCENT=52.632
PRIMER_LEFT_187_SELF_ANY=8.00
PRIMER_RIGHT_187_SELF_ANY=3.00
PRIMER_LEFT_187_SELF_END=2.00
PRIMER_RIGHT_187_SELF_END=0.00
PRIMER_LEFT_187_END_STABILITY=6.7000
PRIMER_RIGHT_187_END_STABILITY=8.2000
PRIMER_PAIR_187_COMPL_ANY=4.00
PRIMER_PAIR_187_COMPL_END=3.00
PRIMER_PAIR_187_PRODUCT_SIZE=80
PRIMER_PAIR_187_PRODUCT_TM=72.4
PRIMER_PAIR_188_PENALTY=3.852497
PRIMER_LEFT_188_PENALTY=2.222066
PRIMER_RIGHT_188_PENALTY=1.630431
PRIMER_LEFT_188_SEQUENCE=GGAGGTACCCACTGATGGTAA
PRIMER_RIGHT_188_SEQUENCE=TGTGCATGTTCAGTCTGCC
PRIMER_LEFT_188=253,21
PRIMER_RIGHT_188=332,19
PRIMER_LEFT_188_TM=58.778
PRIMER_RIGHT_188_TM=59.370
PRIMER_LEFT_188_GC_PERCENT=52.381
PRIMER_RIGHT_188_GC_PERCENT=52.632
PRIMER_LEFT_188_SELF_ANY=6.00
PRIMER_RIGHT_188_SELF_ANY=5.00
PRIMER_LEFT_188_SELF_END=3.00
PRIMER_RIGHT_188_SELF_END=1.00
PRIMER_LEFT_188_END_STABILITY=7.2000
PRIMER_RIGHT_188_END_STABILITY=9.7000
PRIMER_PAIR_188_COMPL_ANY=5.00
PRIMER_PAIR_188_COMPL_END=1.00
PRIMER_PAIR_188_PRODUCT_SIZE=80
PRIMER_PAIR_188_PRODUCT_TM=75.0
PRIMER_PAIR_189_PENALTY=3.857288
PRIMER_LEFT_189_PENALTY=1.512710
PRIMER_RIGHT_189_PENALTY=2.344578
PRIMER_LEFT_189_SEQUENCE=TGAATGTCCAGAATGGGAAG
PRIMER_RIGHT_189_SEQUENCE=CAGGATGCCTTCCTTGGTA
PRIMER_LEFT_189=333,20
PRIMER_RIGHT_189=409,19
PRIMER_LEFT_189_TM=58.487
PRIMER_RIGHT_189_TM=58.655
PRIMER_LEFT_189_GC_PERCENT=45.000
PRIMER_RIGHT_189_GC_PERCENT=52.632
PRIMER_LEFT_189_SELF_ANY=3.00
PRIMER_RIGHT_189_SELF_ANY=7.00
PRIMER_LEFT_189_SELF_END=0.00
PRIMER_RIGHT_189_SELF_END=2.00
PRIMER_LEFT_189_END_STABILITY=8.2000
PRIMER_RIGHT_189_END_STABILITY=7.2000
PRIMER_PAIR_189_COMPL_ANY=5.00
PRIMER_PAIR_189_COMPL_END=1.00
PRIMER_PAIR_189_PRODUCT_SIZE=77
PRIMER_PAIR_189_PRODUCT_TM=71.8
PRIMER_PAIR_190_PENALTY=3.865253
PRIMER_LEFT_190_PENALTY=1.566355
PRIMER_RIGHT_190_PENALTY=2.298898
PRIMER_LEFT_190_SEQUENCE=AGTGATGCCCTTCTCGTTCC
PRIMER_RIGHT_190_SEQUENCE=TGTGCCAGTGAAGATGAGTTTC
PRIMER_LEFT_190=569,20
PRIMER_RIGHT_190=653,22
PRIMER_LEFT_190_TM=61.566
PRIMER_RIGHT_190_TM=60.299
PRIMER_LEFT_190_GC_PERCENT=55.000
PRIMER_RIGHT_190_GC_PERCENT=45.455
PRIMER_LEFT_190_SELF_ANY=2.00
PRIMER_RIGHT_190_SELF_ANY=3.00
PRIMER_LEFT_190_SELF_END=0.00
PRIMER_RIGHT_190_SELF_END=3.00
PRIMER_LEFT_190_END_STABILITY=7.9000
PRIMER_RIGHT_190_END_STABILITY=6.7000
PRIMER_PAIR_190_COMPL_ANY=4.00
PRIMER_PAIR_190_COMPL_END=0.00
PRIMER_PAIR_190_PRODUCT_SIZE=85
PRIMER_PAIR_190_PRODUCT_TM=72.1
PRIMER_PAIR_191_PENALTY=3.880111
PRIMER_LEFT_191_PENALTY=2.127449
PRIMER_RIGHT_191_PENALTY=1.752662
PRIMER_LEFT_191_SEQUENCE=AGACCCATCCCCACTTTGTG
PRIMER_RIGHT_191_SEQUENCE=CAGGAACGAGAAGGGCATC
PRIMER_LEFT_191=516,20
PRIMER_RIGHT_191=590,19
PRIMER_LEFT_191_TM=62.127
PRIMER_RIGHT_191_TM=60.753
PRIMER_LEFT_191_GC_PERCENT=55.000
PRIMER_RIGHT_191_GC_PERCENT=57.895
PRIMER_LEFT_191_SELF_ANY=3.00
PRIMER_RIGHT_191_SELF_ANY=2.00
PRIMER_LEFT_191_SELF_END=3.00
PRIMER_RIGHT_191_SELF_END=2.00
PRIMER_LEFT_191_END_STABILITY=7.0000
PRIMER_RIGHT_191_END_STABILITY=8.1000
PRIMER_PAIR_191_COMPL_ANY=4.00
PRIMER_PAIR_191_COMPL_END=1.00
PRIMER_PAIR_191_PRODUCT_SIZE=75
PRIMER_PAIR_191_PRODUCT_TM=72.7
PRIMER_PAIR_192_PENALTY=3.893190
PRIMER_LEFT_192_PENALTY=1.557214
PRIMER_RIGHT_192_PENALTY=2.335976
PRIMER_LEFT_192_SEQUENCE=GCGAAACTCATCTTCACTGG
PRIMER_RIGHT_192_SEQUENCE=ACATGCCGTAGTCATGCAA
PRIMER_LEFT_192=630,20
PRIMER_RIGHT_192=710,19
PRIMER_LEFT_192_TM=58.443
PRIMER_RIGHT_192_TM=58.664
PRIMER_LEFT_192_GC_PERCENT=50.000
PRIMER_RIGHT_192_GC_PERCENT=47.368
PRIMER_LEFT_192_SELF_ANY=3.00
PRIMER_RIGHT_192_SELF_ANY=6.00
PRIMER_LEFT_192_SELF_END=1.00
PRIMER_RIGHT_192_SELF_END=2.00
PRIMER_LEFT_192_END_STABILITY=7.9000
PRIMER_RIGHT_192_END_STABILITY=8.8000
PRIMER_PAIR_192_COMPL_ANY=3.00
PRIMER_PAIR_192_COMPL_END=1.00
PRIMER_PAIR_192_PRODUCT_SIZE=81
PRIMER_PAIR_192_PRODUCT_TM=73.2
PRIMER_PAIR_193_PENALTY=3.905473
PRIMER_LEFT_193_PENALTY=1.532916
PRIMER_RIGHT_193_PENALTY=2.372557
PRIMER_LEFT_193_SEQUENCE=TGCACATGAATGTCCAGAATG
PRIMER_RIGHT_193_SEQUENCE=CTTCCTTGGTATCAATGCAGGT
PRIMER_LEFT_193=327,21
PRIMER_RIGHT_193=401,22
PRIMER_LEFT_193_TM=60.533
PRIMER_RIGHT_193_TM=60.373
PRIMER_LEFT_193_GC_PERCENT=42.857
PRIMER_RIGHT_193_GC_PERCENT=45.455
PRIMER_LEFT_193_SELF_ANY=8.00
PRIMER_RIGHT_193_SELF_ANY=8.00
PRIMER_LEFT_193_SELF_END=1.00
PRIMER_RIGHT_193_SELF_END=2.00
PRIMER_LEFT_193_END_STABILITY=6.9000
PRIMER_RIGHT_193_END_STABILITY=7.9000
PRIMER_PAIR_193_COMPL_ANY=4.00
PRIMER_PAIR_193_COMPL_END=3.00
PRIMER_PAIR_193_PRODUCT_SIZE=75
PRIMER_PAIR_193_PRODUCT_TM=71.0
PRIMER_PAIR_194_PENALTY=3.906167
PRIMER_LEFT_194_PENALTY=0.828193
PRIMER_RIGHT_194_PENALTY=3.077974
PRIMER_LEFT_194_SEQUENCE=TGGGATTCAGATCCATCAGG
PRIMER_RIGHT_194_SEQUENCE=AGTTCAGGGTAGACTTCTTGGC
PRIMER_LEFT_194=353,20
PRIMER_RIGHT_194=438,22
PRIMER_LEFT_194_TM=60.828
PRIMER_RIGHT_194_TM=58.922
PRIMER_LEFT_194_GC_PERCENT=50.000
PRIMER_RIGHT_194_GC_PERCENT=50.000
PRIMER_LEFT_194_SELF_ANY=5.00
PRIMER_RIGHT_194_SELF_ANY=4.00
PRIMER_LEFT_194_SELF_END=2.00
PRIMER_RIGHT_194_SELF_END=2.00
PRIMER_LEFT_194_END_STABILITY=8.2000
PRIMER_RIGHT_194_END_STABILITY=10.0000
PRIMER_PAIR_194_COMPL_ANY=3.00
PRIMER_PAIR_194_COMPL_END=1.00
PRIMER_PAIR_194_PRODUCT_SIZE=86
PRIMER_PAIR_194_PRODUCT_TM=72.5
PRIMER_PAIR_195_PENALTY=3.919309
PRIMER_LEFT_195_PENALTY=2.692052
PRIMER_RIGHT_195_PENALTY=1.227257
PRIMER_LEFT_195_SEQUENCE=CATTGATACCAAGGAAGGCATC
PRIMER_RIGHT_195_SEQUENCE=CACTGGTTGGTTGGCTTCTA
PRIMER_LEFT_195=385,22
PRIMER_RIGHT_195=475,20
PRIMER_LEFT_195_TM=60.692
PRIMER_RIGHT_195_TM=58.773
PRIMER_LEFT_195_GC_PERCENT=45.455
PRIMER_RIGHT_195_GC_PERCENT=50.000
PRIMER_LEFT_195_SELF_ANY=6.00
PRIMER_RIGHT_195_SELF_ANY=3.00
PRIMER_LEFT_195_SELF_END=3.00
PRIMER_RIGHT_195_SELF_END=2.00
PRIMER_LEFT_195_END_STABILITY=8.1000
PRIMER_RIGHT_195_END_STABILITY=6.0000
PRIMER_PAIR_195_COMPL_ANY=5.00
PRIMER_PAIR_195_COMPL_END=3.00
PRIMER_PAIR_195_PRODUCT_SIZE=91
PRIMER_PAIR_195_PRODUCT_TM=73.1
PRIMER_PAIR_196_PENALTY=3.930760
PRIMER_LEFT_196_PENALTY=2.027792
PRIMER_RIGHT_196_PENALTY=1.902968
PRIMER_LEFT_196_SEQUENCE=ACCAATGTGGTAGAAGCCAAC
PRIMER_RIGHT_196_SEQUENCE=AATCACAAAGTGGGGATGGG
PRIMER_LEFT_196=446,21
PRIMER_RIGHT_196=538,20
PRIMER_LEFT_196_TM=58.972
PRIMER_RIGHT_196_TM=61.903
PRIMER_LEFT_196_GC_PERCENT=47.619
PRIMER_RIGHT_196_GC_PERCENT=50.000
PRIMER_LEFT_196_SELF_ANY=6.00
PRIMER_RIGHT_196_SELF_ANY=4.00
PRIMER_LEFT_196_SELF_END=0.00
PRIMER_RIGHT_196_SELF_END=0.00
PRIMER_LEFT_196_END_STABILITY=8.2000
PRIMER_RIGHT_196_END_STABILITY=9.6000
PRIMER_PAIR_196_COMPL_ANY=4.00
PRIMER_PAIR_196_COMPL_END=2.00
PRIMER_PAIR_196_PRODUCT_SIZE=93
PRIMER_PAIR_196_PRODUCT_TM=75.9
PRIMER_PAIR_197_PENALTY=3.930997
PRIMER_LEFT_197_PENALTY=2.618102
PRIMER_RIGHT_197_PENALTY=1.312895
PRIMER_LEFT_197_SEQUENCE=CTGAACCCCAGATTGCCA
PRIMER_RIGHT_197_SEQUENCE=CTGAATCCCACTTCCCATTCT
PRIMER_LEFT_197=288,18
PRIMER_RIGHT_197=362,21
PRIMER_LEFT_197_TM=60.618
PRIMER_RIGHT_197_TM=60.313
PRIMER_LEFT_197_GC_PERCENT=55.556
PRIMER_RIGHT_197_GC_PERCENT=47.619
PRIMER_LEFT_197_SELF_ANY=3.00
PRIMER_RIGHT_197_SELF_ANY=4.00
PRIMER_LEFT_197_SELF_END=3.00
PRIMER_RIGHT_197_SELF_END=0.00
PRIMER_LEFT_197_END_STABILITY=10.0000
PRIMER_RIGHT_197_END_STABILITY=6.6000
PRIMER_PAIR_197_COMPL_ANY=4.00
PRIMER_PAIR_197_COMPL_END=3.00
PRIMER_PAIR_197_PRODUCT_SIZE=75
PRIMER_PAIR_197_PRODUCT_TM=72.1
PRIMER_PAIR_198_PENALTY=3.933587
PRIMER_LEFT_198_PENALTY=1.883110
PRIMER_RIGHT_198_PENALTY=2.050476
PRIMER_LEFT_198_SEQUENCE=ATCTTCACTGGCACACCGT
PRIMER_RIGHT_198_SEQUENCE=ACTTGTCAATTCCGCAGGG
PRIMER_LEFT_198=639,19
PRIMER_RIGHT_198=734,19
PRIMER_LEFT_198_TM=59.117
PRIMER_RIGHT_198_TM=61.050
PRIMER_LEFT_198_GC_PERCENT=52.632
PRIMER_RIGHT_198_GC_PERCENT=52.632
PRIMER_LEFT_198_SELF_ANY=3.00
PRIMER_RIGHT_198_SELF_ANY=5.00
PRIMER_LEFT_198_SELF_END=3.00
PRIMER_RIGHT_198_SELF_END=1.00
PRIMER_LEFT_198_END_STABILITY=9.3000
PRIMER_RIGHT_198_END_STABILITY=9.7000
PRIMER_PAIR_198_COMPL_ANY=3.00
PRIMER_PAIR_198_COMPL_END=2.00
PRIMER_PAIR_198_PRODUCT_SIZE=96
PRIMER_PAIR_198_PRODUCT_TM=74.6
PRIMER_PAIR_199_PENALTY=3.944577
PRIMER_LEFT_199_PENALTY=1.616405
PRIMER_RIGHT_199_PENALTY=2.328173
PRIMER_LEFT_199_SEQUENCE=GTGATGCCCTTCTCGTTCC
PRIMER_RIGHT_199_SEQUENCE=CGGTGTGCCAGTGAAGATG
PRIMER_LEFT_199=570,19
PRIMER_RIGHT_199=656,19
PRIMER_LEFT_199_TM=60.616
PRIMER_RIGHT_199_TM=61.328
PRIMER_LEFT_199_GC_PERCENT=57.895
PRIMER_RIGHT_199_GC_PERCENT=57.895
PRIMER_LEFT_199_SELF_ANY=2.00
PRIMER_RIGHT_199_SELF_ANY=3.00
PRIMER_LEFT_199_SELF_END=0.00
PRIMER_RIGHT_199_SELF_END=0.00
PRIMER_LEFT_199_END_STABILITY=7.9000
PRIMER_RIGHT_199_END_STABILITY=6.6000
PRIMER_PAIR_199_COMPL_ANY=4.00
PRIMER_PAIR_199_COMPL_END=1.00
PRIMER_PAIR_199_PRODUCT_SIZE=87
PRIMER_PAIR_199_PRODUCT_TM=73.3
PRIMER_PAIR_200_PENALTY=3.944972
PRIMER_LEFT_200_PENALTY=2.192310
PRIMER_RIGHT_200_PENALTY=1.752662
PRIMER_LEFT_200_SEQUENCE=GACCCATCCCCACTTTGTG
PRIMER_RIGHT_200_SEQUENCE=TCAGGAACGAGAAGGGCAT
PRIMER_LEFT_200=517,19
PRIMER_RIGHT_200=591,19
PRIMER_LEFT_200_TM=61.192
PRIMER_RIGHT_200_TM=60.753
PRIMER_LEFT_200_GC_PERCENT=57.895
PRIMER_RIGHT_200_GC_PERCENT=52.632
PRIMER_LEFT_200_SELF_ANY=3.00
PRIMER_RIGHT_200_SELF_ANY=2.00
PRIMER_LEFT_200_SELF_END=3.00
PRIMER_RIGHT_200_SELF_END=2.00
PRIMER_LEFT_200_END_STABILITY=7.0000
PRIMER_RIGHT_200_END_STABILITY=9.6000
PRIMER_PAIR_200_COMPL_ANY=4.00
PRIMER_PAIR_200_COMPL_END=2.00
PRIMER_PAIR_200_PRODUCT_SIZE=75
PRIMER_PAIR_200_PRODUCT_TM=72.7
PRIMER_PAIR_201_PENALTY=3.953976
PRIMER_LEFT_201_PENALTY=2.018039
PRIMER_RIGHT_201_PENALTY=1.935937
PRIMER_LEFT_201_SEQUENCE=ATTCCCTACCGCTGCTTAGT
PRIMER_RIGHT_201_SEQUENCE=TTTCGCAAACATCCATCCT
PRIMER_LEFT_201=536,20
PRIMER_RIGHT_201=635,19
PRIMER_LEFT_201_TM=57.982
PRIMER_RIGHT_201_TM=59.064
PRIMER_LEFT_201_GC_PERCENT=50.000
PRIMER_RIGHT_201_GC_PERCENT=42.105
PRIMER_LEFT_201_SELF_ANY=3.00
PRIMER_RIGHT_201_SELF_ANY=4.00
PRIMER_LEFT_201_SELF_END=1.00
PRIMER_RIGHT_201_SELF_END=0.00
PRIMER_LEFT_201_END_STABILITY=5.7000
PRIMER_RIGHT_201_END_STABILITY=7.8000
PRIMER_PAIR_201_COMPL_ANY=3.00
PRIMER_PAIR_201_COMPL_END=1.00
PRIMER_PAIR_201_PRODUCT_SIZE=100
PRIMER_PAIR_201_PRODUCT_TM=72.8
PRIMER_PAIR_202_PENALTY=3.965341
PRIMER_LEFT_202_PENALTY=1.752662
PRIMER_RIGHT_202_PENALTY=2.212679
PRIMER_LEFT_202_SEQUENCE=ATGCCCTTCTCGTTCCTGA
PRIMER_RIGHT_202_SEQUENCE=ATGTCTCTTTGGCGACGG
PRIMER_LEFT_202=573,19
PRIMER_RIGHT_202=671,18
PRIMER_LEFT_202_TM=60.753
PRIMER_RIGHT_202_TM=59.787
PRIMER_LEFT_202_GC_PERCENT=52.632
PRIMER_RIGHT_202_GC_PERCENT=55.556
PRIMER_LEFT_202_SELF_ANY=2.00
PRIMER_RIGHT_202_SELF_ANY=3.00
PRIMER_LEFT_202_SELF_END=2.00
PRIMER_RIGHT_202_SELF_END=1.00
PRIMER_LEFT_202_END_STABILITY=8.2000
PRIMER_RIGHT_202_END_STABILITY=9.6000
PRIMER_PAIR_202_COMPL_ANY=3.00
PRIMER_PAIR_202_COMPL_END=0.00
PRIMER_PAIR_202_PRODUCT_SIZE=99
PRIMER_PAIR_202_PRODUCT_TM=73.7
PRIMER_PAIR_203_PENALTY=3.975396
PRIMER_LEFT_203_PENALTY=2.618102
PRIMER_RIGHT_203_PENALTY=1.357294
PRIMER_LEFT_203_SEQUENCE=TGGCTGAACCCCAGATTG
PRIMER_RIGHT_203_SEQUENCE=ATCTGAATCCCACTTCCCATT
PRIMER_LEFT_203=285,18
PRIMER_RIGHT_203=364,21
PRIMER_LEFT_203_TM=60.618
PRIMER_RIGHT_203_TM=59.643
PRIMER_LEFT_203_GC_PERCENT=55.556
PRIMER_RIGHT_203_GC_PERCENT=42.857
PRIMER_LEFT_203_SELF_ANY=3.00
PRIMER_RIGHT_203_SELF_ANY=3.00
PRIMER_LEFT_203_SELF_END=2.00
PRIMER_RIGHT_203_SELF_END=1.00
PRIMER_LEFT_203_END_STABILITY=6.9000
PRIMER_RIGHT_203_END_STABILITY=8.4000
PRIMER_PAIR_203_COMPL_ANY=5.00
PRIMER_PAIR_203_COMPL_END=2.00
PRIMER_PAIR_203_PRODUCT_SIZE=80
PRIMER_PAIR_203_PRODUCT_TM=72.4
PRIMER_PAIR_204_PENALTY=3.977161
PRIMER_LEFT_204_PENALTY=2.420393
PRIMER_RIGHT_204_PENALTY=1.556768
PRIMER_LEFT_204_SEQUENCE=TGCCATGTTCTGTGGCAG
PRIMER_RIGHT_204_SEQUENCE=TCCTTGGTATCAATGCAGGTT
PRIMER_LEFT_204=301,18
PRIMER_RIGHT_204=399,21
PRIMER_LEFT_204_TM=60.420
PRIMER_RIGHT_204_TM=59.443
PRIMER_LEFT_204_GC_PERCENT=55.556
PRIMER_RIGHT_204_GC_PERCENT=42.857
PRIMER_LEFT_204_SELF_ANY=5.00
PRIMER_RIGHT_204_SELF_ANY=6.00
PRIMER_LEFT_204_SELF_END=3.00
PRIMER_RIGHT_204_SELF_END=0.00
PRIMER_LEFT_204_END_STABILITY=9.7000
PRIMER_RIGHT_204_END_STABILITY=7.9000
PRIMER_PAIR_204_COMPL_ANY=3.00
PRIMER_PAIR_204_COMPL_END=0.00
PRIMER_PAIR_204_PRODUCT_SIZE=99
PRIMER_PAIR_204_PRODUCT_TM=73.3
PRIMER_PAIR_205_PENALTY=3.980046
PRIMER_LEFT_205_PENALTY=1.595257
PRIMER_RIGHT_205_PENALTY=2.384789
PRIMER_LEFT_205_SEQUENCE=CCCCACTTTGTGATTCCCTAC
PRIMER_RIGHT_205_SEQUENCE=TTTGCACTTGTCAGGAACGAG
PRIMER_LEFT_205=524,21
PRIMER_RIGHT_205=601,21
PRIMER_LEFT_205_TM=60.595
PRIMER_RIGHT_205_TM=61.385
PRIMER_LEFT_205_GC_PERCENT=52.381
PRIMER_RIGHT_205_GC_PERCENT=47.619
PRIMER_LEFT_205_SELF_ANY=3.00
PRIMER_RIGHT_205_SELF_ANY=4.00
PRIMER_LEFT_205_SELF_END=1.00
PRIMER_RIGHT_205_SELF_END=2.00
PRIMER_LEFT_205_END_STABILITY=6.9000
PRIMER_RIGHT_205_END_STABILITY=8.1000
PRIMER_PAIR_205_COMPL_ANY=4.00
PRIMER_PAIR_205_COMPL_END=2.00
PRIMER_PAIR_205_PRODUCT_SIZE=78
PRIMER_PAIR_205_PRODUCT_TM=72.2
PRIMER_PAIR_206_PENALTY=3.995346
PRIMER_LEFT_206_PENALTY=2.402111
PRIMER_RIGHT_206_PENALTY=1.593235
PRIMER_LEFT_206_SEQUENCE=GGTTTGGCACTGCTCCTG
PRIMER_RIGHT_206_SEQUENCE=CAGGCCAGCATTACCATCAG
PRIMER_LEFT_206=209,18
PRIMER_RIGHT_206=283,20
PRIMER_LEFT_206_TM=60.402
PRIMER_RIGHT_206_TM=61.593
PRIMER_LEFT_206_GC_PERCENT=61.111
PRIMER_RIGHT_206_GC_PERCENT=55.000
PRIMER_LEFT_206_SELF_ANY=5.00
PRIMER_RIGHT_206_SELF_ANY=4.00
PRIMER_LEFT_206_SELF_END=1.00
PRIMER_RIGHT_206_SELF_END=1.00
PRIMER_LEFT_206_END_STABILITY=8.2000
PRIMER_RIGHT_206_END_STABILITY=6.6000
PRIMER_PAIR_206_COMPL_ANY=6.00
PRIMER_PAIR_206_COMPL_END=3.00
PRIMER_PAIR_206_PRODUCT_SIZE=75
PRIMER_PAIR_206_PRODUCT_TM=79.2
PRIMER_PAIR_207_PENALTY=4.032768
PRIMER_LEFT_207_PENALTY=1.673968
PRIMER_RIGHT_207_PENALTY=2.358800
PRIMER_LEFT_207_SEQUENCE=TAAGTGATGCCCTTCTCGTTC
PRIMER_RIGHT_207_SEQUENCE=CCAGTGAAGATGAGTTTCGCA
PRIMER_LEFT_207=567,21
PRIMER_RIGHT_207=649,21
PRIMER_LEFT_207_TM=59.326
PRIMER_RIGHT_207_TM=61.359
PRIMER_LEFT_207_GC_PERCENT=47.619
PRIMER_RIGHT_207_GC_PERCENT=47.619
PRIMER_LEFT_207_SELF_ANY=4.00
PRIMER_RIGHT_207_SELF_ANY=3.00
PRIMER_LEFT_207_SELF_END=0.00
PRIMER_RIGHT_207_SELF_END=2.00
PRIMER_LEFT_207_END_STABILITY=8.4000
PRIMER_RIGHT_207_END_STABILITY=10.2000
PRIMER_PAIR_207_COMPL_ANY=4.00
PRIMER_PAIR_207_COMPL_END=1.00
PRIMER_PAIR_207_PRODUCT_SIZE=83
PRIMER_PAIR_207_PRODUCT_TM=71.4
PRIMER_PAIR_208_PENALTY=4.033853
PRIMER_LEFT_208_PENALTY=1.604487
PRIMER_RIGHT_208_PENALTY=2.429366
PRIMER_LEFT_208_SEQUENCE=GAATGTCCAGAATGGGAAGTG
PRIMER_RIGHT_208_SEQUENCE=GCAGGATGCCTTCCTTGGTA
PRIMER_LEFT_208=334,21
PRIMER_RIGHT_208=410,20
PRIMER_LEFT_208_TM=59.396
PRIMER_RIGHT_208_TM=62.429
PRIMER_LEFT_208_GC_PERCENT=47.619
PRIMER_RIGHT_208_GC_PERCENT=55.000
PRIMER_LEFT_208_SELF_ANY=3.00
PRIMER_RIGHT_208_SELF_ANY=7.00
PRIMER_LEFT_208_SELF_END=0.00
PRIMER_RIGHT_208_SELF_END=2.00
PRIMER_LEFT_208_END_STABILITY=6.7000
PRIMER_RIGHT_208_END_STABILITY=7.2000
PRIMER_PAIR_208_COMPL_ANY=5.00
PRIMER_PAIR_208_COMPL_END=0.00
PRIMER_PAIR_208_PRODUCT_SIZE=77
PRIMER_PAIR_208_PRODUCT_TM=72.3
PRIMER_PAIR_209_PENALTY=4.037527
PRIMER_LEFT_209_PENALTY=2.492700
PRIMER_RIGHT_209_PENALTY=1.544828
PRIMER_LEFT_209_SEQUENCE=AACCAGTGACCATCCAGAACT
PRIMER_RIGHT_209_SEQUENCE=TCACCAACTAAGCAGCGGT
PRIMER_LEFT_209=468,21
PRIMER_RIGHT_209=561,19
PRIMER_LEFT_209_TM=58.507
PRIMER_RIGHT_209_TM=59.455
PRIMER_LEFT_209_GC_PERCENT=47.619
PRIMER_RIGHT_209_GC_PERCENT=52.632
PRIMER_LEFT_209_SELF_ANY=3.00
PRIMER_RIGHT_209_SELF_ANY=3.00
PRIMER_LEFT_209_SELF_END=2.00
PRIMER_RIGHT_209_SELF_END=2.00
PRIMER_LEFT_209_END_STABILITY=6.4000
PRIMER_RIGHT_209_END_STABILITY=11.1000
PRIMER_PAIR_209_COMPL_ANY=4.00
PRIMER_PAIR_209_COMPL_END=1.00
PRIMER_PAIR_209_PRODUCT_SIZE=94
PRIMER_PAIR_209_PRODUCT_TM=76.6
PRIMER_PAIR_210_PENALTY=4.039921
PRIMER_LEFT_210_PENALTY=2.290278
PRIMER_RIGHT_210_PENALTY=1.749643
PRIMER_LEFT_210_SEQUENCE=AGAAGCCAACCAACCAGTG
PRIMER_RIGHT_210_SEQUENCE=AAGCAGCGGTAGGGAATCA
PRIMER_LEFT_210=457,19
PRIMER_RIGHT_210=552,19
PRIMER_LEFT_210_TM=58.710
PRIMER_RIGHT_210_TM=60.750
PRIMER_LEFT_210_GC_PERCENT=52.632
PRIMER_RIGHT_210_GC_PERCENT=52.632
PRIMER_LEFT_210_SELF_ANY=3.00
PRIMER_RIGHT_210_SELF_ANY=3.00
PRIMER_LEFT_210_SELF_END=3.00
PRIMER_RIGHT_210_SELF_END=1.00
PRIMER_LEFT_210_END_STABILITY=6.7000
PRIMER_RIGHT_210_END_STABILITY=6.9000
PRIMER_PAIR_210_COMPL_ANY=4.00
PRIMER_PAIR_210_COMPL_END=2.00
PRIMER_PAIR_210_PRODUCT_SIZE=96
PRIMER_PAIR_210_PRODUCT_TM=77.1
PRIMER_PAIR_211_PENALTY=4.052795
PRIMER_LEFT_211_PENALTY=2.066321
PRIMER_RIGHT_211_PENALTY=1.986473
PRIMER_LEFT_211_SEQUENCE=TCGTTCCTGACAAGTGCAA
PRIMER_RIGHT_211_SEQUENCE=TGCATGTCTCTTTGGCGAC
PRIMER_LEFT_211=582,19
PRIMER_RIGHT_211=674,19
PRIMER_LEFT_211_TM=58.934
PRIMER_RIGHT_211_TM=60.986
PRIMER_LEFT_211_GC_PERCENT=47.368
PRIMER_RIGHT_211_GC_PERCENT=52.632
PRIMER_LEFT_211_SELF_ANY=4.00
PRIMER_RIGHT_211_SELF_ANY=4.00
PRIMER_LEFT_211_SELF_END=2.00
PRIMER_RIGHT_211_SELF_END=2.00
PRIMER_LEFT_211_END_STABILITY=8.8000
PRIMER_RIGHT_211_END_STABILITY=9.6000
PRIMER_PAIR_211_COMPL_ANY=4.00
PRIMER_PAIR_211_COMPL_END=2.00
PRIMER_PAIR_211_PRODUCT_SIZE=93
PRIMER_PAIR_211_PRODUCT_TM=73.3
PRIMER_PAIR_212_PENALTY=4.064698
PRIMER_LEFT_212_PENALTY=2.037339
PRIMER_RIGHT_212_PENALTY=2.027359
PRIMER_LEFT_212_SEQUENCE=TGGTGAGTTTGTAAGTGATGCC
PRIMER_RIGHT_212_SEQUENCE=TGAGTTTCGCAAACATCCATC
PRIMER_LEFT_212=556,22
PRIMER_RIGHT_212=639,21
PRIMER_LEFT_212_TM=60.037
PRIMER_RIGHT_212_TM=61.027
PRIMER_LEFT_212_GC_PERCENT=45.455
PRIMER_RIGHT_212_GC_PERCENT=42.857
PRIMER_LEFT_212_SELF_ANY=2.00
PRIMER_RIGHT_212_SELF_ANY=6.00
PRIMER_LEFT_212_SELF_END=1.00
PRIMER_RIGHT_212_SELF_END=0.00
PRIMER_LEFT_212_END_STABILITY=9.6000
PRIMER_RIGHT_212_END_STABILITY=8.1000
PRIMER_PAIR_212_COMPL_ANY=5.00
PRIMER_PAIR_212_COMPL_END=2.00
PRIMER_PAIR_212_PRODUCT_SIZE=84
PRIMER_PAIR_212_PRODUCT_TM=71.3
PRIMER_PAIR_213_PENALTY=4.092033
PRIMER_LEFT_213_PENALTY=1.899540
PRIMER_RIGHT_213_PENALTY=2.192493
PRIMER_LEFT_213_SEQUENCE=TCTTCACTGGCACACCGTC
PRIMER_RIGHT_213_SEQUENCE=CTTGTCAATTCCGCAGGG
PRIMER_LEFT_213=640,19
PRIMER_RIGHT_213=733,18
PRIMER_LEFT_213_TM=60.900
PRIMER_RIGHT_213_TM=60.192
PRIMER_LEFT_213_GC_PERCENT=57.895
PRIMER_RIGHT_213_GC_PERCENT=55.556
PRIMER_LEFT_213_SELF_ANY=3.00
PRIMER_RIGHT_213_SELF_ANY=5.00
PRIMER_LEFT_213_SELF_END=1.00
PRIMER_RIGHT_213_SELF_END=1.00
PRIMER_LEFT_213_END_STABILITY=9.6000
PRIMER_RIGHT_213_END_STABILITY=9.7000
PRIMER_PAIR_213_COMPL_ANY=3.00
PRIMER_PAIR_213_COMPL_END=1.00
PRIMER_PAIR_213_PRODUCT_SIZE=94
PRIMER_PAIR_213_PRODUCT_TM=74.9
PRIMER_PAIR_214_PENALTY=4.092753
PRIMER_LEFT_214_PENALTY=2.130358
PRIMER_RIGHT_214_PENALTY=1.962396
PRIMER_LEFT_214_SEQUENCE=ATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_214_SEQUENCE=GCACCAGTTCTGGATGGTC
PRIMER_LEFT_214=414,22
PRIMER_RIGHT_214=493,19
PRIMER_LEFT_214_TM=60.130
PRIMER_RIGHT_214_TM=59.038
PRIMER_LEFT_214_GC_PERCENT=45.455
PRIMER_RIGHT_214_GC_PERCENT=57.895
PRIMER_LEFT_214_SELF_ANY=5.00
PRIMER_RIGHT_214_SELF_ANY=6.00
PRIMER_LEFT_214_SELF_END=1.00
PRIMER_RIGHT_214_SELF_END=3.00
PRIMER_LEFT_214_END_STABILITY=8.2000
PRIMER_RIGHT_214_END_STABILITY=7.9000
PRIMER_PAIR_214_COMPL_ANY=4.00
PRIMER_PAIR_214_COMPL_END=3.00
PRIMER_PAIR_214_PRODUCT_SIZE=80
PRIMER_PAIR_214_PRODUCT_TM=72.9
PRIMER_PAIR_215_PENALTY=4.098568
PRIMER_LEFT_215_PENALTY=2.174817
PRIMER_RIGHT_215_PENALTY=1.923751
PRIMER_LEFT_215_SEQUENCE=ACCAAAACCTGCATTGATACC
PRIMER_RIGHT_215_SEQUENCE=TTGGTTGGCTTCTACCACAT
PRIMER_LEFT_215=374,21
PRIMER_RIGHT_215=469,20
PRIMER_LEFT_215_TM=58.825
PRIMER_RIGHT_215_TM=58.076
PRIMER_LEFT_215_GC_PERCENT=42.857
PRIMER_RIGHT_215_GC_PERCENT=45.000
PRIMER_LEFT_215_SELF_ANY=4.00
PRIMER_RIGHT_215_SELF_ANY=4.00
PRIMER_LEFT_215_SELF_END=2.00
PRIMER_RIGHT_215_SELF_END=2.00
PRIMER_LEFT_215_END_STABILITY=6.8000
PRIMER_RIGHT_215_END_STABILITY=6.6000
PRIMER_PAIR_215_COMPL_ANY=5.00
PRIMER_PAIR_215_COMPL_END=1.00
PRIMER_PAIR_215_PRODUCT_SIZE=96
PRIMER_PAIR_215_PRODUCT_TM=72.9
PRIMER_PAIR_216_PENALTY=4.102484
PRIMER_LEFT_216_PENALTY=2.486079
PRIMER_RIGHT_216_PENALTY=1.616405
PRIMER_LEFT_216_SEQUENCE=CAAGCAGTGCAAGACCCA
PRIMER_RIGHT_216_SEQUENCE=GGAACGAGAAGGGCATCAC
PRIMER_LEFT_216=505,18
PRIMER_RIGHT_216=588,19
PRIMER_LEFT_216_TM=59.514
PRIMER_RIGHT_216_TM=60.616
PRIMER_LEFT_216_GC_PERCENT=55.556
PRIMER_RIGHT_216_GC_PERCENT=57.895
PRIMER_LEFT_216_SELF_ANY=5.00
PRIMER_RIGHT_216_SELF_ANY=2.00
PRIMER_LEFT_216_SELF_END=0.00
PRIMER_RIGHT_216_SELF_END=2.00
PRIMER_LEFT_216_END_STABILITY=9.4000
PRIMER_RIGHT_216_END_STABILITY=6.3000
PRIMER_PAIR_216_COMPL_ANY=3.00
PRIMER_PAIR_216_COMPL_END=2.00
PRIMER_PAIR_216_PRODUCT_SIZE=84
PRIMER_PAIR_216_PRODUCT_TM=73.7
PRIMER_PAIR_217_PENALTY=4.109720
PRIMER_LEFT_217_PENALTY=2.024215
PRIMER_RIGHT_217_PENALTY=2.085505
PRIMER_LEFT_217_SEQUENCE=CCCTACCGCTGCTTAGTTGG
PRIMER_RIGHT_217_SEQUENCE=GTTTCGCAAACATCCATCC
PRIMER_LEFT_217=539,20
PRIMER_RIGHT_217=636,19
PRIMER_LEFT_217_TM=62.024
PRIMER_RIGHT_217_TM=58.914
PRIMER_LEFT_217_GC_PERCENT=60.000
PRIMER_RIGHT_217_GC_PERCENT=47.368
PRIMER_LEFT_217_SELF_ANY=3.00
PRIMER_RIGHT_217_SELF_ANY=6.00
PRIMER_LEFT_217_SELF_END=0.00
PRIMER_RIGHT_217_SELF_END=0.00
PRIMER_LEFT_217_END_STABILITY=8.2000
PRIMER_RIGHT_217_END_STABILITY=8.1000
PRIMER_PAIR_217_COMPL_ANY=3.00
PRIMER_PAIR_217_COMPL_END=2.00
PRIMER_PAIR_217_PRODUCT_SIZE=98
PRIMER_PAIR_217_PRODUCT_TM=73.4
PRIMER_PAIR_218_PENALTY=4.111462
PRIMER_LEFT_218_PENALTY=1.752662
PRIMER_RIGHT_218_PENALTY=2.358800
PRIMER_LEFT_218_SEQUENCE=GATGCCCTTCTCGTTCCTG
PRIMER_RIGHT_218_SEQUENCE=TGCCAGTGAAGATGAGTTTCG
PRIMER_LEFT_218=572,19
PRIMER_RIGHT_218=651,21
PRIMER_LEFT_218_TM=60.753
PRIMER_RIGHT_218_TM=61.359
PRIMER_LEFT_218_GC_PERCENT=57.895
PRIMER_RIGHT_218_GC_PERCENT=47.619
PRIMER_LEFT_218_SELF_ANY=2.00
PRIMER_RIGHT_218_SELF_ANY=3.00
PRIMER_LEFT_218_SELF_END=1.00
PRIMER_RIGHT_218_SELF_END=2.00
PRIMER_LEFT_218_END_STABILITY=8.2000
PRIMER_RIGHT_218_END_STABILITY=9.0000
PRIMER_PAIR_218_COMPL_ANY=4.00
PRIMER_PAIR_218_COMPL_END=0.00
PRIMER_PAIR_218_PRODUCT_SIZE=80
PRIMER_PAIR_218_PRODUCT_TM=71.9
PRIMER_PAIR_219_PENALTY=4.114989
PRIMER_LEFT_219_PENALTY=2.186531
PRIMER_RIGHT_219_PENALTY=1.928458
PRIMER_LEFT_219_SEQUENCE=AGGGACCAAAACCTGCATTG
PRIMER_RIGHT_219_SEQUENCE=TGGTTGGCTTCTACCACATTG
PRIMER_LEFT_219=370,20
PRIMER_RIGHT_219=468,21
PRIMER_LEFT_219_TM=62.187
PRIMER_RIGHT_219_TM=60.928
PRIMER_LEFT_219_GC_PERCENT=50.000
PRIMER_RIGHT_219_GC_PERCENT=47.619
PRIMER_LEFT_219_SELF_ANY=4.00
PRIMER_RIGHT_219_SELF_ANY=4.00
PRIMER_LEFT_219_SELF_END=3.00
PRIMER_RIGHT_219_SELF_END=3.00
PRIMER_LEFT_219_END_STABILITY=7.2000
PRIMER_RIGHT_219_END_STABILITY=7.2000
PRIMER_PAIR_219_COMPL_ANY=4.00
PRIMER_PAIR_219_COMPL_END=3.00
PRIMER_PAIR_219_PRODUCT_SIZE=99
PRIMER_PAIR_219_PRODUCT_TM=73.7
PRIMER_PAIR_220_PENALTY=4.118223
PRIMER_LEFT_220_PENALTY=2.561455
PRIMER_RIGHT_220_PENALTY=1.556768
PRIMER_LEFT_220_SEQUENCE=ATGCACATGAATGTCCAGAA
PRIMER_RIGHT_220_SEQUENCE=TTCCTTGGTATCAATGCAGGT
PRIMER_LEFT_220=326,20
PRIMER_RIGHT_220=400,21
PRIMER_LEFT_220_TM=57.439
PRIMER_RIGHT_220_TM=59.443
PRIMER_LEFT_220_GC_PERCENT=40.000
PRIMER_RIGHT_220_GC_PERCENT=42.857
PRIMER_LEFT_220_SELF_ANY=8.00
PRIMER_RIGHT_220_SELF_ANY=6.00
PRIMER_LEFT_220_SELF_END=1.00
PRIMER_RIGHT_220_SELF_END=0.00
PRIMER_LEFT_220_END_STABILITY=7.0000
PRIMER_RIGHT_220_END_STABILITY=7.9000
PRIMER_PAIR_220_COMPL_ANY=4.00
PRIMER_PAIR_220_COMPL_END=2.00
PRIMER_PAIR_220_PRODUCT_SIZE=75
PRIMER_PAIR_220_PRODUCT_TM=70.5
PRIMER_PAIR_221_PENALTY=4.120357
PRIMER_LEFT_221_PENALTY=2.074556
PRIMER_RIGHT_221_PENALTY=2.045801
PRIMER_LEFT_221_SEQUENCE=ATCACCAATGTGGTAGAAGCC
PRIMER_RIGHT_221_SEQUENCE=GGGATGGGTCTTGCACTG
PRIMER_LEFT_221=443,21
PRIMER_RIGHT_221=526,18
PRIMER_LEFT_221_TM=58.925
PRIMER_RIGHT_221_TM=60.046
PRIMER_LEFT_221_GC_PERCENT=47.619
PRIMER_RIGHT_221_GC_PERCENT=61.111
PRIMER_LEFT_221_SELF_ANY=6.00
PRIMER_RIGHT_221_SELF_ANY=4.00
PRIMER_LEFT_221_SELF_END=1.00
PRIMER_RIGHT_221_SELF_END=3.00
PRIMER_LEFT_221_END_STABILITY=9.7000
PRIMER_RIGHT_221_END_STABILITY=6.7000
PRIMER_PAIR_221_COMPL_ANY=4.00
PRIMER_PAIR_221_COMPL_END=1.00
PRIMER_PAIR_221_PRODUCT_SIZE=84
PRIMER_PAIR_221_PRODUCT_TM=76.2
PRIMER_PAIR_222_PENALTY=4.130443
PRIMER_LEFT_222_PENALTY=2.402111
PRIMER_RIGHT_222_PENALTY=1.728332
PRIMER_LEFT_222_SEQUENCE=CACCGTCGCCAAAGAGAC
PRIMER_RIGHT_222_SEQUENCE=TACCCCTCGGAACTTGTCAAT
PRIMER_LEFT_222=652,18
PRIMER_RIGHT_222=745,21
PRIMER_LEFT_222_TM=60.402
PRIMER_RIGHT_222_TM=60.728
PRIMER_LEFT_222_GC_PERCENT=61.111
PRIMER_RIGHT_222_GC_PERCENT=47.619
PRIMER_LEFT_222_SELF_ANY=3.00
PRIMER_RIGHT_222_SELF_ANY=5.00
PRIMER_LEFT_222_SELF_END=2.00
PRIMER_RIGHT_222_SELF_END=3.00
PRIMER_LEFT_222_END_STABILITY=6.1000
PRIMER_RIGHT_222_END_STABILITY=6.9000
PRIMER_PAIR_222_COMPL_ANY=3.00
PRIMER_PAIR_222_COMPL_END=2.00
PRIMER_PAIR_222_PRODUCT_SIZE=94
PRIMER_PAIR_222_PRODUCT_TM=75.3
PRIMER_PAIR_223_PENALTY=4.147260
PRIMER_LEFT_223_PENALTY=2.402111
PRIMER_RIGHT_223_PENALTY=1.745148
PRIMER_LEFT_223_SEQUENCE=ACACCGTCGCCAAAGAGA
PRIMER_RIGHT_223_SEQUENCE=CTCTACCCCTCGGAACTTGT
PRIMER_LEFT_223=651,18
PRIMER_RIGHT_223=748,20
PRIMER_LEFT_223_TM=60.402
PRIMER_RIGHT_223_TM=58.255
PRIMER_LEFT_223_GC_PERCENT=55.556
PRIMER_RIGHT_223_GC_PERCENT=55.000
PRIMER_LEFT_223_SELF_ANY=3.00
PRIMER_RIGHT_223_SELF_ANY=3.00
PRIMER_LEFT_223_SELF_END=0.00
PRIMER_RIGHT_223_SELF_END=2.00
PRIMER_LEFT_223_END_STABILITY=6.4000
PRIMER_RIGHT_223_END_STABILITY=6.7000
PRIMER_PAIR_223_COMPL_ANY=4.00
PRIMER_PAIR_223_COMPL_END=1.00
PRIMER_PAIR_223_PRODUCT_SIZE=98
PRIMER_PAIR_223_PRODUCT_TM=75.5
PRIMER_PAIR_224_PENALTY=4.154774
PRIMER_LEFT_224_PENALTY=1.752662
PRIMER_RIGHT_224_PENALTY=2.402111
PRIMER_LEFT_224_SEQUENCE=TGATGCCCTTCTCGTTCCT
PRIMER_RIGHT_224_SEQUENCE=TCTCTTTGGCGACGGTGT
PRIMER_LEFT_224=571,19
PRIMER_RIGHT_224=668,18
PRIMER_LEFT_224_TM=60.753
PRIMER_RIGHT_224_TM=60.402
PRIMER_LEFT_224_GC_PERCENT=52.632
PRIMER_RIGHT_224_GC_PERCENT=55.556
PRIMER_LEFT_224_SELF_ANY=2.00
PRIMER_RIGHT_224_SELF_ANY=3.00
PRIMER_LEFT_224_SELF_END=0.00
PRIMER_RIGHT_224_SELF_END=1.00
PRIMER_LEFT_224_END_STABILITY=8.2000
PRIMER_RIGHT_224_END_STABILITY=7.6000
PRIMER_PAIR_224_COMPL_ANY=3.00
PRIMER_PAIR_224_COMPL_END=0.00
PRIMER_PAIR_224_PRODUCT_SIZE=98
PRIMER_PAIR_224_PRODUCT_TM=73.9
PRIMER_PAIR_225_PENALTY=4.157833
PRIMER_LEFT_225_PENALTY=1.651669
PRIMER_RIGHT_225_PENALTY=2.506164
PRIMER_LEFT_225_SEQUENCE=TCCCCACTTTGTGATTCCCT
PRIMER_RIGHT_225_SEQUENCE=TGCACTTGTCAGGAACGAG
PRIMER_LEFT_225=523,20
PRIMER_RIGHT_225=599,19
PRIMER_LEFT_225_TM=61.652
PRIMER_RIGHT_225_TM=58.494
PRIMER_LEFT_225_GC_PERCENT=50.000
PRIMER_RIGHT_225_GC_PERCENT=52.632
PRIMER_LEFT_225_SELF_ANY=3.00
PRIMER_RIGHT_225_SELF_ANY=4.00
PRIMER_LEFT_225_SELF_END=1.00
PRIMER_RIGHT_225_SELF_END=2.00
PRIMER_LEFT_225_END_STABILITY=9.4000
PRIMER_RIGHT_225_END_STABILITY=8.1000
PRIMER_PAIR_225_COMPL_ANY=4.00
PRIMER_PAIR_225_COMPL_END=2.00
PRIMER_PAIR_225_PRODUCT_SIZE=77
PRIMER_PAIR_225_PRODUCT_TM=72.3
PRIMER_PAIR_226_PENALTY=4.205638
PRIMER_LEFT_226_PENALTY=2.282356
PRIMER_RIGHT_226_PENALTY=1.923282
PRIMER_LEFT_226_SEQUENCE=AATGTGGTAGAAGCCAACCAAC
PRIMER_RIGHT_226_SEQUENCE=CGGTAGGGAATCACAAAGTG
PRIMER_LEFT_226=449,22
PRIMER_RIGHT_226=546,20
PRIMER_LEFT_226_TM=60.282
PRIMER_RIGHT_226_TM=58.077
PRIMER_LEFT_226_GC_PERCENT=45.455
PRIMER_RIGHT_226_GC_PERCENT=50.000
PRIMER_LEFT_226_SELF_ANY=4.00
PRIMER_RIGHT_226_SELF_ANY=3.00
PRIMER_LEFT_226_SELF_END=2.00
PRIMER_RIGHT_226_SELF_END=3.00
PRIMER_LEFT_226_END_STABILITY=8.2000
PRIMER_RIGHT_226_END_STABILITY=6.7000
PRIMER_PAIR_226_COMPL_ANY=4.00
PRIMER_PAIR_226_COMPL_END=1.00
PRIMER_PAIR_226_PRODUCT_SIZE=98
PRIMER_PAIR_226_PRODUCT_TM=76.8
PRIMER_PAIR_227_PENALTY=4.221294
PRIMER_LEFT_227_PENALTY=2.547327
PRIMER_RIGHT_227_PENALTY=1.673968
PRIMER_LEFT_227_SEQUENCE=AAGCAGTGCAAGACCCATCC
PRIMER_RIGHT_227_SEQUENCE=GAACGAGAAGGGCATCACTTA
PRIMER_LEFT_227=506,20
PRIMER_RIGHT_227=587,21
PRIMER_LEFT_227_TM=62.547
PRIMER_RIGHT_227_TM=59.326
PRIMER_LEFT_227_GC_PERCENT=55.000
PRIMER_RIGHT_227_GC_PERCENT=47.619
PRIMER_LEFT_227_SELF_ANY=5.00
PRIMER_RIGHT_227_SELF_ANY=4.00
PRIMER_LEFT_227_SELF_END=0.00
PRIMER_RIGHT_227_SELF_END=2.00
PRIMER_LEFT_227_END_STABILITY=8.1000
PRIMER_RIGHT_227_END_STABILITY=5.7000
PRIMER_PAIR_227_COMPL_ANY=5.00
PRIMER_PAIR_227_COMPL_END=2.00
PRIMER_PAIR_227_PRODUCT_SIZE=82
PRIMER_PAIR_227_PRODUCT_TM=73.1
PRIMER_PAIR_228_PENALTY=4.221881
PRIMER_LEFT_228_PENALTY=2.617395
PRIMER_RIGHT_228_PENALTY=1.604487
PRIMER_LEFT_228_SEQUENCE=GTACCCACTGATGGTAATGCTG
PRIMER_RIGHT_228_SEQUENCE=CACTTCCCATTCTGGACATTC
PRIMER_LEFT_228=257,22
PRIMER_RIGHT_228=354,21
PRIMER_LEFT_228_TM=59.383
PRIMER_RIGHT_228_TM=59.396
PRIMER_LEFT_228_GC_PERCENT=50.000
PRIMER_RIGHT_228_GC_PERCENT=47.619
PRIMER_LEFT_228_SELF_ANY=5.00
PRIMER_RIGHT_228_SELF_ANY=3.00
PRIMER_LEFT_228_SELF_END=1.00
PRIMER_RIGHT_228_SELF_END=2.00
PRIMER_LEFT_228_END_STABILITY=8.5000
PRIMER_RIGHT_228_END_STABILITY=6.9000
PRIMER_PAIR_228_COMPL_ANY=5.00
PRIMER_PAIR_228_COMPL_END=1.00
PRIMER_PAIR_228_PRODUCT_SIZE=98
PRIMER_PAIR_228_PRODUCT_TM=75.1
PRIMER_PAIR_229_PENALTY=4.232776
PRIMER_LEFT_229_PENALTY=2.994694
PRIMER_RIGHT_229_PENALTY=1.238082
PRIMER_LEFT_229_SEQUENCE=ATTGATACCAAGGAAGGCATC
PRIMER_RIGHT_229_SEQUENCE=GATGGTCACTGGTTGGTTGG
PRIMER_LEFT_229=386,21
PRIMER_RIGHT_229=481,20
PRIMER_LEFT_229_TM=58.005
PRIMER_RIGHT_229_TM=61.238
PRIMER_LEFT_229_GC_PERCENT=42.857
PRIMER_RIGHT_229_GC_PERCENT=55.000
PRIMER_LEFT_229_SELF_ANY=6.00
PRIMER_RIGHT_229_SELF_ANY=3.00
PRIMER_LEFT_229_SELF_END=3.00
PRIMER_RIGHT_229_SELF_END=0.00
PRIMER_LEFT_229_END_STABILITY=8.1000
PRIMER_RIGHT_229_END_STABILITY=8.2000
PRIMER_PAIR_229_COMPL_ANY=5.00
PRIMER_PAIR_229_COMPL_END=1.00
PRIMER_PAIR_229_PRODUCT_SIZE=96
PRIMER_PAIR_229_PRODUCT_TM=73.3
PRIMER_PAIR_230_PENALTY=4.286251
PRIMER_LEFT_230_PENALTY=2.215444
PRIMER_RIGHT_230_PENALTY=2.070807
PRIMER_LEFT_230_SEQUENCE=CCCTTCTCGTTCCTGACAA
PRIMER_RIGHT_230_SEQUENCE=TGCATGTCTCTTTGGCGA
PRIMER_LEFT_230=576,19
PRIMER_RIGHT_230=674,18
PRIMER_LEFT_230_TM=58.785
PRIMER_RIGHT_230_TM=60.071
PRIMER_LEFT_230_GC_PERCENT=52.632
PRIMER_RIGHT_230_GC_PERCENT=50.000
PRIMER_LEFT_230_SELF_ANY=3.00
PRIMER_RIGHT_230_SELF_ANY=4.00
PRIMER_LEFT_230_SELF_END=1.00
PRIMER_RIGHT_230_SELF_END=2.00
PRIMER_LEFT_230_END_STABILITY=6.7000
PRIMER_RIGHT_230_END_STABILITY=11.4000
PRIMER_PAIR_230_COMPL_ANY=4.00
PRIMER_PAIR_230_COMPL_END=2.00
PRIMER_PAIR_230_PRODUCT_SIZE=99
PRIMER_PAIR_230_PRODUCT_TM=74.1
PRIMER_PAIR_231_PENALTY=4.308743
PRIMER_LEFT_231_PENALTY=2.567306
PRIMER_RIGHT_231_PENALTY=1.741437
PRIMER_LEFT_231_SEQUENCE=GTTTGGCACTGCTCCTGC
PRIMER_RIGHT_231_SEQUENCE=ACATGGCAATCTGGGGTTC
PRIMER_LEFT_231=210,18
PRIMER_RIGHT_231=308,19
PRIMER_LEFT_231_TM=60.567
PRIMER_RIGHT_231_TM=60.741
PRIMER_LEFT_231_GC_PERCENT=61.111
PRIMER_RIGHT_231_GC_PERCENT=52.632
PRIMER_LEFT_231_SELF_ANY=5.00
PRIMER_RIGHT_231_SELF_ANY=4.00
PRIMER_LEFT_231_SELF_END=2.00
PRIMER_RIGHT_231_SELF_END=0.00
PRIMER_LEFT_231_END_STABILITY=9.7000
PRIMER_RIGHT_231_END_STABILITY=7.9000
PRIMER_PAIR_231_COMPL_ANY=4.00
PRIMER_PAIR_231_COMPL_END=0.00
PRIMER_PAIR_231_PRODUCT_SIZE=99
PRIMER_PAIR_231_PRODUCT_TM=79.9
PRIMER_PAIR_232_PENALTY=4.314672
PRIMER_LEFT_232_PENALTY=1.709051
PRIMER_RIGHT_232_PENALTY=2.605621
PRIMER_LEFT_232_SEQUENCE=TCCCCACTTTGTGATTCCC
PRIMER_RIGHT_232_SEQUENCE=TTTGCACTTGTCAGGAACG
PRIMER_LEFT_232=523,19
PRIMER_RIGHT_232=601,19
PRIMER_LEFT_232_TM=60.709
PRIMER_RIGHT_232_TM=58.394
PRIMER_LEFT_232_GC_PERCENT=52.632
PRIMER_RIGHT_232_GC_PERCENT=47.368
PRIMER_LEFT_232_SELF_ANY=3.00
PRIMER_RIGHT_232_SELF_ANY=4.00
PRIMER_LEFT_232_SELF_END=3.00
PRIMER_RIGHT_232_SELF_END=2.00
PRIMER_LEFT_232_END_STABILITY=9.7000
PRIMER_RIGHT_232_END_STABILITY=8.4000
PRIMER_PAIR_232_COMPL_ANY=4.00
PRIMER_PAIR_232_COMPL_END=1.00
PRIMER_PAIR_232_PRODUCT_SIZE=79
PRIMER_PAIR_232_PRODUCT_TM=72.0
PRIMER_PAIR_233_PENALTY=4.314902
PRIMER_LEFT_233_PENALTY=2.296862
PRIMER_RIGHT_233_PENALTY=2.018039
PRIMER_LEFT_233_SEQUENCE=AAGCCAACCAACCAGTGACC
PRIMER_RIGHT_233_SEQUENCE=ACTAAGCAGCGGTAGGGAAT
PRIMER_LEFT_233=459,20
PRIMER_RIGHT_233=555,20
PRIMER_LEFT_233_TM=62.297
PRIMER_RIGHT_233_TM=57.982
PRIMER_LEFT_233_GC_PERCENT=55.000
PRIMER_RIGHT_233_GC_PERCENT=50.000
PRIMER_LEFT_233_SELF_ANY=3.00
PRIMER_RIGHT_233_SELF_ANY=3.00
PRIMER_LEFT_233_SELF_END=1.00
PRIMER_RIGHT_233_SELF_END=2.00
PRIMER_LEFT_233_END_STABILITY=7.9000
PRIMER_RIGHT_233_END_STABILITY=8.1000
PRIMER_PAIR_233_COMPL_ANY=4.00
PRIMER_PAIR_233_COMPL_END=0.00
PRIMER_PAIR_233_PRODUCT_SIZE=97
PRIMER_PAIR_233_PRODUCT_TM=77.0
PRIMER_PAIR_234_PENALTY=4.315389
PRIMER_LEFT_234_PENALTY=2.413667
PRIMER_RIGHT_234_PENALTY=1.901722
PRIMER_LEFT_234_SEQUENCE=CGCTGGAGGTACCCACTGAT
PRIMER_RIGHT_234_SEQUENCE=TCATGTGCATGTTCAGTCTGC
PRIMER_LEFT_234=249,20
PRIMER_RIGHT_234=335,21
PRIMER_LEFT_234_TM=62.414
PRIMER_RIGHT_234_TM=60.902
PRIMER_LEFT_234_GC_PERCENT=60.000
PRIMER_RIGHT_234_GC_PERCENT=47.619
PRIMER_LEFT_234_SELF_ANY=6.00
PRIMER_RIGHT_234_SELF_ANY=6.00
PRIMER_LEFT_234_SELF_END=2.00
PRIMER_RIGHT_234_SELF_END=3.00
PRIMER_LEFT_234_END_STABILITY=6.6000
PRIMER_RIGHT_234_END_STABILITY=8.2000
PRIMER_PAIR_234_COMPL_ANY=5.00
PRIMER_PAIR_234_COMPL_END=2.00
PRIMER_PAIR_234_PRODUCT_SIZE=87
PRIMER_PAIR_234_PRODUCT_TM=75.6
PRIMER_PAIR_235_PENALTY=4.318474
PRIMER_LEFT_235_PENALTY=2.942733
PRIMER_RIGHT_235_PENALTY=1.375741
PRIMER_LEFT_235_SEQUENCE=CGCCAAAGAGACATGCAG
PRIMER_RIGHT_235_SEQUENCE=CAAACTCTACCCCTCGGAACT
PRIMER_LEFT_235=658,18
PRIMER_RIGHT_235=752,21
PRIMER_LEFT_235_TM=59.057
PRIMER_RIGHT_235_TM=59.624
PRIMER_LEFT_235_GC_PERCENT=55.556
PRIMER_RIGHT_235_GC_PERCENT=52.381
PRIMER_LEFT_235_SELF_ANY=4.00
PRIMER_RIGHT_235_SELF_ANY=3.00
PRIMER_LEFT_235_SELF_END=2.00
PRIMER_RIGHT_235_SELF_END=1.00
PRIMER_LEFT_235_END_STABILITY=8.5000
PRIMER_RIGHT_235_END_STABILITY=6.4000
PRIMER_PAIR_235_COMPL_ANY=4.00
PRIMER_PAIR_235_COMPL_END=2.00
PRIMER_PAIR_235_PRODUCT_SIZE=95
PRIMER_PAIR_235_PRODUCT_TM=74.7
PRIMER_PAIR_236_PENALTY=4.320583
PRIMER_LEFT_236_PENALTY=2.292791
PRIMER_RIGHT_236_PENALTY=2.027792
PRIMER_LEFT_236_SEQUENCE=GGGACCAAAACCTGCATTG
PRIMER_RIGHT_236_SEQUENCE=GTTGGTTGGCTTCTACCACAT
PRIMER_LEFT_236=371,19
PRIMER_RIGHT_236=470,21
PRIMER_LEFT_236_TM=61.293
PRIMER_RIGHT_236_TM=58.972
PRIMER_LEFT_236_GC_PERCENT=52.632
PRIMER_RIGHT_236_GC_PERCENT=47.619
PRIMER_LEFT_236_SELF_ANY=4.00
PRIMER_RIGHT_236_SELF_ANY=4.00
PRIMER_LEFT_236_SELF_END=3.00
PRIMER_RIGHT_236_SELF_END=2.00
PRIMER_LEFT_236_END_STABILITY=7.2000
PRIMER_RIGHT_236_END_STABILITY=6.6000
PRIMER_PAIR_236_COMPL_ANY=5.00
PRIMER_PAIR_236_COMPL_END=2.00
PRIMER_PAIR_236_PRODUCT_SIZE=100
PRIMER_PAIR_236_PRODUCT_TM=74.0
PRIMER_PAIR_237_PENALTY=4.340715
PRIMER_LEFT_237_PENALTY=1.190908
PRIMER_RIGHT_237_PENALTY=3.149807
PRIMER_LEFT_237_SEQUENCE=ACCAGGAGAGGATGGATGTTT
PRIMER_RIGHT_237_SEQUENCE=TGCCGTAGTCATGCAAGTT
PRIMER_LEFT_237=609,21
PRIMER_RIGHT_237=707,19
PRIMER_LEFT_237_TM=59.809
PRIMER_RIGHT_237_TM=57.850
PRIMER_LEFT_237_GC_PERCENT=47.619
PRIMER_RIGHT_237_GC_PERCENT=47.368
PRIMER_LEFT_237_SELF_ANY=3.00
PRIMER_RIGHT_237_SELF_ANY=4.00
PRIMER_LEFT_237_SELF_END=0.00
PRIMER_RIGHT_237_SELF_END=3.00
PRIMER_LEFT_237_END_STABILITY=7.0000
PRIMER_RIGHT_237_END_STABILITY=6.7000
PRIMER_PAIR_237_COMPL_ANY=4.00
PRIMER_PAIR_237_COMPL_END=1.00
PRIMER_PAIR_237_PRODUCT_SIZE=99
PRIMER_PAIR_237_PRODUCT_TM=74.5
PRIMER_PAIR_238_PENALTY=4.348500
PRIMER_LEFT_238_PENALTY=0.949861
PRIMER_RIGHT_238_PENALTY=3.398638
PRIMER_LEFT_238_SEQUENCE=ATCCATCAGGGACCAAAACC
PRIMER_RIGHT_238_SEQUENCE=TCTGCAGTTCAGGGTAGACTTC
PRIMER_LEFT_238=363,20
PRIMER_RIGHT_238=443,22
PRIMER_LEFT_238_TM=60.950
PRIMER_RIGHT_238_TM=58.601
PRIMER_LEFT_238_GC_PERCENT=50.000
PRIMER_RIGHT_238_GC_PERCENT=50.000
PRIMER_LEFT_238_SELF_ANY=5.00
PRIMER_RIGHT_238_SELF_ANY=6.00
PRIMER_LEFT_238_SELF_END=0.00
PRIMER_RIGHT_238_SELF_END=3.00
PRIMER_LEFT_238_END_STABILITY=8.2000
PRIMER_RIGHT_238_END_STABILITY=6.4000
PRIMER_PAIR_238_COMPL_ANY=3.00
PRIMER_PAIR_238_COMPL_END=0.00
PRIMER_PAIR_238_PRODUCT_SIZE=81
PRIMER_PAIR_238_PRODUCT_TM=72.2
PRIMER_PAIR_239_PENALTY=4.377818
PRIMER_LEFT_239_PENALTY=2.253482
PRIMER_RIGHT_239_PENALTY=2.124336
PRIMER_LEFT_239_SEQUENCE=TGCAAATTCTTACACCAGGAGA
PRIMER_RIGHT_239_SEQUENCE=CTGCATGTCTCTTTGGCGA
PRIMER_LEFT_239=596,22
PRIMER_RIGHT_239=675,19
PRIMER_LEFT_239_TM=59.747
PRIMER_RIGHT_239_TM=61.124
PRIMER_LEFT_239_GC_PERCENT=40.909
PRIMER_RIGHT_239_GC_PERCENT=52.632
PRIMER_LEFT_239_SELF_ANY=4.00
PRIMER_RIGHT_239_SELF_ANY=4.00
PRIMER_LEFT_239_SELF_END=1.00
PRIMER_RIGHT_239_SELF_END=2.00
PRIMER_LEFT_239_END_STABILITY=7.9000
PRIMER_RIGHT_239_END_STABILITY=11.4000
PRIMER_PAIR_239_COMPL_ANY=4.00
PRIMER_PAIR_239_COMPL_END=3.00
PRIMER_PAIR_239_PRODUCT_SIZE=80
PRIMER_PAIR_239_PRODUCT_TM=72.4
PRIMER_PAIR_240_PENALTY=4.400163
PRIMER_LEFT_240_PENALTY=3.162081
PRIMER_RIGHT_240_PENALTY=1.238082
PRIMER_LEFT_240_SEQUENCE=CCTGCAGTATTGCCAAGAAGTC
PRIMER_RIGHT_240_SEQUENCE=GGATGGTCACTGGTTGGTTG
PRIMER_LEFT_240=406,22
PRIMER_RIGHT_240=482,20
PRIMER_LEFT_240_TM=61.162
PRIMER_RIGHT_240_TM=61.238
PRIMER_LEFT_240_GC_PERCENT=50.000
PRIMER_RIGHT_240_GC_PERCENT=55.000
PRIMER_LEFT_240_SELF_ANY=6.00
PRIMER_RIGHT_240_SELF_ANY=3.00
PRIMER_LEFT_240_SELF_END=2.00
PRIMER_RIGHT_240_SELF_END=0.00
PRIMER_LEFT_240_END_STABILITY=6.4000
PRIMER_RIGHT_240_END_STABILITY=8.2000
PRIMER_PAIR_240_COMPL_ANY=5.00
PRIMER_PAIR_240_COMPL_END=1.00
PRIMER_PAIR_240_PRODUCT_SIZE=77
PRIMER_PAIR_240_PRODUCT_TM=72.9
PRIMER_PAIR_241_PENALTY=4.400163
PRIMER_LEFT_241_PENALTY=3.162081
PRIMER_RIGHT_241_PENALTY=1.238082
PRIMER_LEFT_241_SEQUENCE=TCCTGCAGTATTGCCAAGAAGT
PRIMER_RIGHT_241_SEQUENCE=TGGATGGTCACTGGTTGGTT
PRIMER_LEFT_241=405,22
PRIMER_RIGHT_241=483,20
PRIMER_LEFT_241_TM=61.162
PRIMER_RIGHT_241_TM=61.238
PRIMER_LEFT_241_GC_PERCENT=45.455
PRIMER_RIGHT_241_GC_PERCENT=50.000
PRIMER_LEFT_241_SELF_ANY=6.00
PRIMER_RIGHT_241_SELF_ANY=3.00
PRIMER_LEFT_241_SELF_END=1.00
PRIMER_RIGHT_241_SELF_END=0.00
PRIMER_LEFT_241_END_STABILITY=6.4000
PRIMER_RIGHT_241_END_STABILITY=8.2000
PRIMER_PAIR_241_COMPL_ANY=5.00
PRIMER_PAIR_241_COMPL_END=3.00
PRIMER_PAIR_241_PRODUCT_SIZE=79
PRIMER_PAIR_241_PRODUCT_TM=72.5
PRIMER_PAIR_242_PENALTY=4.407754
PRIMER_LEFT_242_PENALTY=2.192310
PRIMER_RIGHT_242_PENALTY=2.215444
PRIMER_LEFT_242_SEQUENCE=ACCCATCCCCACTTTGTGA
PRIMER_RIGHT_242_SEQUENCE=TTGTCAGGAACGAGAAGGG
PRIMER_LEFT_242=518,19
PRIMER_RIGHT_242=594,19
PRIMER_LEFT_242_TM=61.192
PRIMER_RIGHT_242_TM=58.785
PRIMER_LEFT_242_GC_PERCENT=52.632
PRIMER_RIGHT_242_GC_PERCENT=52.632
PRIMER_LEFT_242_SELF_ANY=3.00
PRIMER_RIGHT_242_SELF_ANY=3.00
PRIMER_LEFT_242_SELF_END=2.00
PRIMER_RIGHT_242_SELF_END=0.00
PRIMER_LEFT_242_END_STABILITY=6.7000
PRIMER_RIGHT_242_END_STABILITY=9.7000
PRIMER_PAIR_242_COMPL_ANY=4.00
PRIMER_PAIR_242_COMPL_END=2.00
PRIMER_PAIR_242_PRODUCT_SIZE=77
PRIMER_PAIR_242_PRODUCT_TM=72.3
PRIMER_PAIR_243_PENALTY=4.410865
PRIMER_LEFT_243_PENALTY=1.923282
PRIMER_RIGHT_243_PENALTY=2.487583
PRIMER_LEFT_243_SEQUENCE=CACTTTGTGATTCCCTACCG
PRIMER_RIGHT_243_SEQUENCE=CCATCCTCTCCTGGTGTAAGAA
PRIMER_LEFT_243=527,20
PRIMER_RIGHT_243=623,22
PRIMER_LEFT_243_TM=58.077
PRIMER_RIGHT_243_TM=60.488
PRIMER_LEFT_243_GC_PERCENT=50.000
PRIMER_RIGHT_243_GC_PERCENT=50.000
PRIMER_LEFT_243_SELF_ANY=3.00
PRIMER_RIGHT_243_SELF_ANY=3.00
PRIMER_LEFT_243_SELF_END=2.00
PRIMER_RIGHT_243_SELF_END=1.00
PRIMER_LEFT_243_END_STABILITY=8.9000
PRIMER_RIGHT_243_END_STABILITY=7.0000
PRIMER_PAIR_243_COMPL_ANY=3.00
PRIMER_PAIR_243_COMPL_END=1.00
PRIMER_PAIR_243_PRODUCT_SIZE=97
PRIMER_PAIR_243_PRODUCT_TM=73.2
PRIMER_PAIR_244_PENALTY=4.424294
PRIMER_LEFT_244_PENALTY=3.348683
PRIMER_RIGHT_244_PENALTY=1.075611
PRIMER_LEFT_244_SEQUENCE=CACTGATGGTAATGCTGGCCT
PRIMER_RIGHT_244_SEQUENCE=AATCCCACTTCCCATTCTGG
PRIMER_LEFT_244=262,21
PRIMER_RIGHT_244=359,20
PRIMER_LEFT_244_TM=62.349
PRIMER_RIGHT_244_TM=61.076
PRIMER_LEFT_244_GC_PERCENT=52.381
PRIMER_RIGHT_244_GC_PERCENT=50.000
PRIMER_LEFT_244_SELF_ANY=4.00
PRIMER_RIGHT_244_SELF_ANY=3.00
PRIMER_LEFT_244_SELF_END=2.00
PRIMER_RIGHT_244_SELF_END=3.00
PRIMER_LEFT_244_END_STABILITY=10.9000
PRIMER_RIGHT_244_END_STABILITY=8.2000
PRIMER_PAIR_244_COMPL_ANY=5.00
PRIMER_PAIR_244_COMPL_END=2.00
PRIMER_PAIR_244_PRODUCT_SIZE=98
PRIMER_PAIR_244_PRODUCT_TM=74.7
PRIMER_PAIR_245_PENALTY=4.431970
PRIMER_LEFT_245_PENALTY=2.619082
PRIMER_RIGHT_245_PENALTY=1.812888
PRIMER_LEFT_245_SEQUENCE=AACCAGTGACCATCCAGAAC
PRIMER_RIGHT_245_SEQUENCE=AACTCACCAACTAAGCAGCG
PRIMER_LEFT_245=468,20
PRIMER_RIGHT_245=564,20
PRIMER_LEFT_245_TM=57.381
PRIMER_RIGHT_245_TM=58.187
PRIMER_LEFT_245_GC_PERCENT=50.000
PRIMER_RIGHT_245_GC_PERCENT=50.000
PRIMER_LEFT_245_SELF_ANY=3.00
PRIMER_RIGHT_245_SELF_ANY=3.00
PRIMER_LEFT_245_SELF_END=0.00
PRIMER_RIGHT_245_SELF_END=2.00
PRIMER_LEFT_245_END_STABILITY=6.4000
PRIMER_RIGHT_245_END_STABILITY=10.2000
PRIMER_PAIR_245_COMPL_ANY=4.00
PRIMER_PAIR_245_COMPL_END=1.00
PRIMER_PAIR_245_PRODUCT_SIZE=97
PRIMER_PAIR_245_PRODUCT_TM=76.5
PRIMER_PAIR_246_PENALTY=4.451941
PRIMER_LEFT_246_PENALTY=2.710504
PRIMER_RIGHT_246_PENALTY=1.741437
PRIMER_LEFT_246_SEQUENCE=TTTGGCACTGCTCCTGCT
PRIMER_RIGHT_246_SEQUENCE=GAACATGGCAATCTGGGGT
PRIMER_LEFT_246=211,18
PRIMER_RIGHT_246=310,19
PRIMER_LEFT_246_TM=60.711
PRIMER_RIGHT_246_TM=60.741
PRIMER_LEFT_246_GC_PERCENT=55.556
PRIMER_RIGHT_246_GC_PERCENT=52.632
PRIMER_LEFT_246_SELF_ANY=5.00
PRIMER_RIGHT_246_SELF_ANY=4.00
PRIMER_LEFT_246_SELF_END=0.00
PRIMER_RIGHT_246_SELF_END=0.00
PRIMER_LEFT_246_END_STABILITY=8.2000
PRIMER_RIGHT_246_END_STABILITY=10.6000
PRIMER_PAIR_246_COMPL_ANY=5.00
PRIMER_PAIR_246_COMPL_END=1.00
PRIMER_PAIR_246_PRODUCT_SIZE=100
PRIMER_PAIR_246_PRODUCT_TM=79.7
PRIMER_PAIR_247_PENALTY=4.458236
PRIMER_LEFT_247_PENALTY=2.167720
PRIMER_RIGHT_247_PENALTY=2.290516
PRIMER_LEFT_247_SEQUENCE=CATCTTCACTGGCACACCGT
PRIMER_RIGHT_247_SEQUENCE=AACTTGTCAATTCCGCAGG
PRIMER_LEFT_247=638,20
PRIMER_RIGHT_247=735,19
PRIMER_LEFT_247_TM=62.168
PRIMER_RIGHT_247_TM=58.709
PRIMER_LEFT_247_GC_PERCENT=55.000
PRIMER_RIGHT_247_GC_PERCENT=47.368
PRIMER_LEFT_247_SELF_ANY=3.00
PRIMER_RIGHT_247_SELF_ANY=5.00
PRIMER_LEFT_247_SELF_END=3.00
PRIMER_RIGHT_247_SELF_END=2.00
PRIMER_LEFT_247_END_STABILITY=9.3000
PRIMER_RIGHT_247_END_STABILITY=9.7000
PRIMER_PAIR_247_COMPL_ANY=3.00
PRIMER_PAIR_247_COMPL_END=1.00
PRIMER_PAIR_247_PRODUCT_SIZE=98
PRIMER_PAIR_247_PRODUCT_TM=74.7
PRIMER_PAIR_248_PENALTY=4.471214
PRIMER_LEFT_248_PENALTY=2.446999
PRIMER_RIGHT_248_PENALTY=2.024215
PRIMER_LEFT_248_SEQUENCE=AAGCCAACCAACCAGTGAC
PRIMER_RIGHT_248_SEQUENCE=CCAACTAAGCAGCGGTAGGG
PRIMER_LEFT_248=459,19
PRIMER_RIGHT_248=558,20
PRIMER_LEFT_248_TM=58.553
PRIMER_RIGHT_248_TM=62.024
PRIMER_LEFT_248_GC_PERCENT=52.632
PRIMER_RIGHT_248_GC_PERCENT=60.000
PRIMER_LEFT_248_SELF_ANY=3.00
PRIMER_RIGHT_248_SELF_ANY=3.00
PRIMER_LEFT_248_SELF_END=3.00
PRIMER_RIGHT_248_SELF_END=0.00
PRIMER_LEFT_248_END_STABILITY=6.1000
PRIMER_RIGHT_248_END_STABILITY=8.7000
PRIMER_PAIR_248_COMPL_ANY=4.00
PRIMER_PAIR_248_COMPL_END=2.00
PRIMER_PAIR_248_PRODUCT_SIZE=100
PRIMER_PAIR_248_PRODUCT_TM=77.3
PRIMER_PAIR_249_PENALTY=4.476003
PRIMER_LEFT_249_PENALTY=2.738851
PRIMER_RIGHT_249_PENALTY=1.737152
PRIMER_LEFT_249_SEQUENCE=CAGACTGAACATGCACATGAA
PRIMER_RIGHT_249_SEQUENCE=TGGTATCAATGCAGGTTTTGG
PRIMER_LEFT_249=316,21
PRIMER_RIGHT_249=395,21
PRIMER_LEFT_249_TM=58.261
PRIMER_RIGHT_249_TM=60.737
PRIMER_LEFT_249_GC_PERCENT=42.857
PRIMER_RIGHT_249_GC_PERCENT=42.857
PRIMER_LEFT_249_SELF_ANY=6.00
PRIMER_RIGHT_249_SELF_ANY=4.00
PRIMER_LEFT_249_SELF_END=2.00
PRIMER_RIGHT_249_SELF_END=0.00
PRIMER_LEFT_249_END_STABILITY=6.9000
PRIMER_RIGHT_249_END_STABILITY=8.8000
PRIMER_PAIR_249_COMPL_ANY=6.00
PRIMER_PAIR_249_COMPL_END=3.00
PRIMER_PAIR_249_PRODUCT_SIZE=80
PRIMER_PAIR_249_PRODUCT_TM=71.4
PRIMER_PAIR_250_PENALTY=4.535334
PRIMER_LEFT_250_PENALTY=2.240419
PRIMER_RIGHT_250_PENALTY=2.294916
PRIMER_LEFT_250_SEQUENCE=ACCGCTGCTTAGTTGGTGAGT
PRIMER_RIGHT_250_SEQUENCE=AACATCCATCCTCTCCTGGTG
PRIMER_LEFT_250=543,21
PRIMER_RIGHT_250=628,21
PRIMER_LEFT_250_TM=61.240
PRIMER_RIGHT_250_TM=61.295
PRIMER_LEFT_250_GC_PERCENT=52.381
PRIMER_RIGHT_250_GC_PERCENT=52.381
PRIMER_LEFT_250_SELF_ANY=3.00
PRIMER_RIGHT_250_SELF_ANY=3.00
PRIMER_LEFT_250_SELF_END=1.00
PRIMER_RIGHT_250_SELF_END=3.00
PRIMER_LEFT_250_END_STABILITY=6.4000
PRIMER_RIGHT_250_END_STABILITY=8.2000
PRIMER_PAIR_250_COMPL_ANY=4.00
PRIMER_PAIR_250_COMPL_END=0.00
PRIMER_PAIR_250_PRODUCT_SIZE=86
PRIMER_PAIR_250_PRODUCT_TM=72.0
PRIMER_PAIR_251_PENALTY=4.550826
PRIMER_LEFT_251_PENALTY=3.135712
PRIMER_RIGHT_251_PENALTY=1.415114
PRIMER_LEFT_251_SEQUENCE=TATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_251_SEQUENCE=GTTCTGGATGGTCACTGGTTG
PRIMER_LEFT_251=413,23
PRIMER_RIGHT_251=487,21
PRIMER_LEFT_251_TM=60.136
PRIMER_RIGHT_251_TM=60.415
PRIMER_LEFT_251_GC_PERCENT=43.478
PRIMER_RIGHT_251_GC_PERCENT=52.381
PRIMER_LEFT_251_SELF_ANY=5.00
PRIMER_RIGHT_251_SELF_ANY=3.00
PRIMER_LEFT_251_SELF_END=1.00
PRIMER_RIGHT_251_SELF_END=0.00
PRIMER_LEFT_251_END_STABILITY=8.2000
PRIMER_RIGHT_251_END_STABILITY=8.2000
PRIMER_PAIR_251_COMPL_ANY=4.00
PRIMER_PAIR_251_COMPL_END=3.00
PRIMER_PAIR_251_PRODUCT_SIZE=75
PRIMER_PAIR_251_PRODUCT_TM=71.6
PRIMER_PAIR_252_PENALTY=4.564315
PRIMER_LEFT_252_PENALTY=2.887640
PRIMER_RIGHT_252_PENALTY=1.676675
PRIMER_LEFT_252_SEQUENCE=TTGCCATGTTCTGTGGCAG
PRIMER_RIGHT_252_SEQUENCE=GGTCCCTGATGGATCTGAATC
PRIMER_LEFT_252=300,19
PRIMER_RIGHT_252=376,21
PRIMER_LEFT_252_TM=61.888
PRIMER_RIGHT_252_TM=60.677
PRIMER_LEFT_252_GC_PERCENT=52.632
PRIMER_RIGHT_252_GC_PERCENT=52.381
PRIMER_LEFT_252_SELF_ANY=5.00
PRIMER_RIGHT_252_SELF_ANY=5.00
PRIMER_LEFT_252_SELF_END=3.00
PRIMER_RIGHT_252_SELF_END=3.00
PRIMER_LEFT_252_END_STABILITY=9.7000
PRIMER_RIGHT_252_END_STABILITY=6.6000
PRIMER_PAIR_252_COMPL_ANY=4.00
PRIMER_PAIR_252_COMPL_END=1.00
PRIMER_PAIR_252_PRODUCT_SIZE=77
PRIMER_PAIR_252_PRODUCT_TM=72.3
PRIMER_PAIR_253_PENALTY=4.571775
PRIMER_LEFT_253_PENALTY=2.444325
PRIMER_RIGHT_253_PENALTY=2.127449
PRIMER_LEFT_253_SEQUENCE=GGTAGAAGCCAACCAACCA
PRIMER_RIGHT_253_SEQUENCE=CACAAAGTGGGGATGGGTCT
PRIMER_LEFT_253=454,19
PRIMER_RIGHT_253=535,20
PRIMER_LEFT_253_TM=58.556
PRIMER_RIGHT_253_TM=62.127
PRIMER_LEFT_253_GC_PERCENT=52.632
PRIMER_RIGHT_253_GC_PERCENT=55.000
PRIMER_LEFT_253_SELF_ANY=3.00
PRIMER_RIGHT_253_SELF_ANY=3.00
PRIMER_LEFT_253_SELF_END=0.00
PRIMER_RIGHT_253_SELF_END=0.00
PRIMER_LEFT_253_END_STABILITY=8.2000
PRIMER_RIGHT_253_END_STABILITY=7.6000
PRIMER_PAIR_253_COMPL_ANY=4.00
PRIMER_PAIR_253_COMPL_END=2.00
PRIMER_PAIR_253_PRODUCT_SIZE=82
PRIMER_PAIR_253_PRODUCT_TM=76.6
PRIMER_PAIR_254_PENALTY=4.592141
PRIMER_LEFT_254_PENALTY=3.239726
PRIMER_RIGHT_254_PENALTY=1.352415
PRIMER_LEFT_254_SEQUENCE=GCATTGATACCAAGGAAGGCA
PRIMER_RIGHT_254_SEQUENCE=CACTGGTTGGTTGGCTTCTAC
PRIMER_LEFT_254=384,21
PRIMER_RIGHT_254=475,21
PRIMER_LEFT_254_TM=62.240
PRIMER_RIGHT_254_TM=59.648
PRIMER_LEFT_254_GC_PERCENT=47.619
PRIMER_RIGHT_254_GC_PERCENT=52.381
PRIMER_LEFT_254_SELF_ANY=6.00
PRIMER_RIGHT_254_SELF_ANY=3.00
PRIMER_LEFT_254_SELF_END=0.00
PRIMER_RIGHT_254_SELF_END=0.00
PRIMER_LEFT_254_END_STABILITY=9.7000
PRIMER_RIGHT_254_END_STABILITY=5.4000
PRIMER_PAIR_254_COMPL_ANY=5.00
PRIMER_PAIR_254_COMPL_END=2.00
PRIMER_PAIR_254_PRODUCT_SIZE=92
PRIMER_PAIR_254_PRODUCT_TM=73.4
PRIMER_PAIR_255_PENALTY=4.598043
PRIMER_LEFT_255_PENALTY=2.137489
PRIMER_RIGHT_255_PENALTY=2.460554
PRIMER_LEFT_255_SEQUENCE=GTAAGTGATGCCCTTCTCGTTC
PRIMER_RIGHT_255_SEQUENCE=CAGTGAAGATGAGTTTCGCA
PRIMER_LEFT_255=566,22
PRIMER_RIGHT_255=648,20
PRIMER_LEFT_255_TM=60.137
PRIMER_RIGHT_255_TM=57.539
PRIMER_LEFT_255_GC_PERCENT=50.000
PRIMER_RIGHT_255_GC_PERCENT=45.000
PRIMER_LEFT_255_SELF_ANY=4.00
PRIMER_RIGHT_255_SELF_ANY=3.00
PRIMER_LEFT_255_SELF_END=2.00
PRIMER_RIGHT_255_SELF_END=2.00
PRIMER_LEFT_255_END_STABILITY=8.4000
PRIMER_RIGHT_255_END_STABILITY=10.2000
PRIMER_PAIR_255_COMPL_ANY=4.00
PRIMER_PAIR_255_COMPL_END=1.00
PRIMER_PAIR_255_PRODUCT_SIZE=83
PRIMER_PAIR_255_PRODUCT_TM=71.4
PRIMER_PAIR_256_PENALTY=4.636635
PRIMER_LEFT_256_PENALTY=2.444325
PRIMER_RIGHT_256_PENALTY=2.192310
PRIMER_LEFT_256_SEQUENCE=TGGTAGAAGCCAACCAACC
PRIMER_RIGHT_256_SEQUENCE=CACAAAGTGGGGATGGGTC
PRIMER_LEFT_256=453,19
PRIMER_RIGHT_256=535,19
PRIMER_LEFT_256_TM=58.556
PRIMER_RIGHT_256_TM=61.192
PRIMER_LEFT_256_GC_PERCENT=52.632
PRIMER_RIGHT_256_GC_PERCENT=57.895
PRIMER_LEFT_256_SELF_ANY=4.00
PRIMER_RIGHT_256_SELF_ANY=3.00
PRIMER_LEFT_256_SELF_END=0.00
PRIMER_RIGHT_256_SELF_END=1.00
PRIMER_LEFT_256_END_STABILITY=8.2000
PRIMER_RIGHT_256_END_STABILITY=9.1000
PRIMER_PAIR_256_COMPL_ANY=4.00
PRIMER_PAIR_256_COMPL_END=3.00
PRIMER_PAIR_256_PRODUCT_SIZE=83
PRIMER_PAIR_256_PRODUCT_TM=76.4
PRIMER_PAIR_257_PENALTY=4.644527
PRIMER_LEFT_257_PENALTY=2.328173
PRIMER_RIGHT_257_PENALTY=2.316355
PRIMER_LEFT_257_SEQUENCE=CATCTTCACTGGCACACCG
PRIMER_RIGHT_257_SEQUENCE=AACTTGTCAATTCCGCAGGG
PRIMER_LEFT_257=638,19
PRIMER_RIGHT_257=735,20
PRIMER_LEFT_257_TM=61.328
PRIMER_RIGHT_257_TM=62.316
PRIMER_LEFT_257_GC_PERCENT=57.895
PRIMER_RIGHT_257_GC_PERCENT=50.000
PRIMER_LEFT_257_SELF_ANY=3.00
PRIMER_RIGHT_257_SELF_ANY=5.00
PRIMER_LEFT_257_SELF_END=2.00
PRIMER_RIGHT_257_SELF_END=1.00
PRIMER_LEFT_257_END_STABILITY=9.9000
PRIMER_RIGHT_257_END_STABILITY=9.7000
PRIMER_PAIR_257_COMPL_ANY=3.00
PRIMER_PAIR_257_COMPL_END=1.00
PRIMER_PAIR_257_PRODUCT_SIZE=98
PRIMER_PAIR_257_PRODUCT_TM=74.7
PRIMER_PAIR_258_PENALTY=4.660248
PRIMER_LEFT_258_PENALTY=2.477524
PRIMER_RIGHT_258_PENALTY=2.182723
PRIMER_LEFT_258_SEQUENCE=TCACCAATGTGGTAGAAGCCA
PRIMER_RIGHT_258_SEQUENCE=GATGGGTCTTGCACTGCTT
PRIMER_LEFT_258=444,21
PRIMER_RIGHT_258=524,19
PRIMER_LEFT_258_TM=61.478
PRIMER_RIGHT_258_TM=58.817
PRIMER_LEFT_258_GC_PERCENT=47.619
PRIMER_RIGHT_258_GC_PERCENT=52.632
PRIMER_LEFT_258_SELF_ANY=6.00
PRIMER_RIGHT_258_SELF_ANY=5.00
PRIMER_LEFT_258_SELF_END=1.00
PRIMER_RIGHT_258_SELF_END=1.00
PRIMER_LEFT_258_END_STABILITY=9.7000
PRIMER_RIGHT_258_END_STABILITY=8.5000
PRIMER_PAIR_258_COMPL_ANY=4.00
PRIMER_PAIR_258_COMPL_END=3.00
PRIMER_PAIR_258_PRODUCT_SIZE=81
PRIMER_PAIR_258_PRODUCT_TM=75.8
PRIMER_PAIR_259_PENALTY=4.664552
PRIMER_LEFT_259_PENALTY=2.305331
PRIMER_RIGHT_259_PENALTY=2.359221
PRIMER_LEFT_259_SEQUENCE=CCCATCCCCACTTTGTGA
PRIMER_RIGHT_259_SEQUENCE=TGTCAGGAACGAGAAGGGC
PRIMER_LEFT_259=519,18
PRIMER_RIGHT_259=593,19
PRIMER_LEFT_259_TM=60.305
PRIMER_RIGHT_259_TM=61.359
PRIMER_LEFT_259_GC_PERCENT=55.556
PRIMER_RIGHT_259_GC_PERCENT=57.895
PRIMER_LEFT_259_SELF_ANY=3.00
PRIMER_RIGHT_259_SELF_ANY=3.00
PRIMER_LEFT_259_SELF_END=2.00
PRIMER_RIGHT_259_SELF_END=2.00
PRIMER_LEFT_259_END_STABILITY=6.7000
PRIMER_RIGHT_259_END_STABILITY=10.9000
PRIMER_PAIR_259_COMPL_ANY=4.00
PRIMER_PAIR_259_COMPL_END=2.00
PRIMER_PAIR_259_PRODUCT_SIZE=75
PRIMER_PAIR_259_PRODUCT_TM=72.7
PRIMER_PAIR_260_PENALTY=4.684530
PRIMER_LEFT_260_PENALTY=2.282418
PRIMER_RIGHT_260_PENALTY=2.402111
PRIMER_LEFT_260_SEQUENCE=TGATGCCCTTCTCGTTCC
PRIMER_RIGHT_260_SEQUENCE=GTCTCTTTGGCGACGGTG
PRIMER_LEFT_260=571,18
PRIMER_RIGHT_260=669,18
PRIMER_LEFT_260_TM=59.718
PRIMER_RIGHT_260_TM=60.402
PRIMER_LEFT_260_GC_PERCENT=55.556
PRIMER_RIGHT_260_GC_PERCENT=61.111
PRIMER_LEFT_260_SELF_ANY=2.00
PRIMER_RIGHT_260_SELF_ANY=3.00
PRIMER_LEFT_260_SELF_END=0.00
PRIMER_RIGHT_260_SELF_END=1.00
PRIMER_LEFT_260_END_STABILITY=7.9000
PRIMER_RIGHT_260_END_STABILITY=9.9000
PRIMER_PAIR_260_COMPL_ANY=3.00
PRIMER_PAIR_260_COMPL_END=1.00
PRIMER_PAIR_260_PRODUCT_SIZE=99
PRIMER_PAIR_260_PRODUCT_TM=74.1
PRIMER_PAIR_261_PENALTY=4.687539
PRIMER_LEFT_261_PENALTY=2.495229
PRIMER_RIGHT_261_PENALTY=2.192310
PRIMER_LEFT_261_SEQUENCE=AGAAGCCAACCAACCAGTGAC
PRIMER_RIGHT_261_SEQUENCE=TCACAAAGTGGGGATGGGT
PRIMER_LEFT_261=457,21
PRIMER_RIGHT_261=536,19
PRIMER_LEFT_261_TM=61.495
PRIMER_RIGHT_261_TM=61.192
PRIMER_LEFT_261_GC_PERCENT=52.381
PRIMER_RIGHT_261_GC_PERCENT=52.632
PRIMER_LEFT_261_SELF_ANY=3.00
PRIMER_RIGHT_261_SELF_ANY=3.00
PRIMER_LEFT_261_SELF_END=3.00
PRIMER_RIGHT_261_SELF_END=0.00
PRIMER_LEFT_261_END_STABILITY=6.1000
PRIMER_RIGHT_261_END_STABILITY=9.4000
PRIMER_PAIR_261_COMPL_ANY=4.00
PRIMER_PAIR_261_COMPL_END=3.00
PRIMER_PAIR_261_PRODUCT_SIZE=80
PRIMER_PAIR_261_PRODUCT_TM=76.0
PRIMER_PAIR_262_PENALTY=4.704795
PRIMER_LEFT_262_PENALTY=2.567306
PRIMER_RIGHT_262_PENALTY=2.137489
PRIMER_LEFT_262_SEQUENCE=GCAAGCAGTGCAAGACCC
PRIMER_RIGHT_262_SEQUENCE=GAACGAGAAGGGCATCACTTAC
PRIMER_LEFT_262=504,18
PRIMER_RIGHT_262=587,22
PRIMER_LEFT_262_TM=60.567
PRIMER_RIGHT_262_TM=60.137
PRIMER_LEFT_262_GC_PERCENT=61.111
PRIMER_RIGHT_262_GC_PERCENT=50.000
PRIMER_LEFT_262_SELF_ANY=5.00
PRIMER_RIGHT_262_SELF_ANY=4.00
PRIMER_LEFT_262_SELF_END=0.00
PRIMER_RIGHT_262_SELF_END=2.00
PRIMER_LEFT_262_END_STABILITY=9.1000
PRIMER_RIGHT_262_END_STABILITY=5.7000
PRIMER_PAIR_262_COMPL_ANY=4.00
PRIMER_PAIR_262_COMPL_END=2.00
PRIMER_PAIR_262_PRODUCT_SIZE=84
PRIMER_PAIR_262_PRODUCT_TM=73.7
PRIMER_PAIR_263_PENALTY=4.716875
PRIMER_LEFT_263_PENALTY=3.088710
PRIMER_RIGHT_263_PENALTY=1.628165
PRIMER_LEFT_263_SEQUENCE=ACCCACTGATGGTAATGCTGG
PRIMER_RIGHT_263_SEQUENCE=ATCCCACTTCCCATTCTGGA
PRIMER_LEFT_263=259,21
PRIMER_RIGHT_263=358,20
PRIMER_LEFT_263_TM=62.089
PRIMER_RIGHT_263_TM=61.628
PRIMER_LEFT_263_GC_PERCENT=52.381
PRIMER_RIGHT_263_GC_PERCENT=50.000
PRIMER_LEFT_263_SELF_ANY=3.00
PRIMER_RIGHT_263_SELF_ANY=3.00
PRIMER_LEFT_263_SELF_END=0.00
PRIMER_RIGHT_263_SELF_END=2.00
PRIMER_LEFT_263_END_STABILITY=9.7000
PRIMER_RIGHT_263_END_STABILITY=8.2000
PRIMER_PAIR_263_COMPL_ANY=5.00
PRIMER_PAIR_263_COMPL_END=3.00
PRIMER_PAIR_263_PRODUCT_SIZE=100
PRIMER_PAIR_263_PRODUCT_TM=75.2
PRIMER_PAIR_264_PENALTY=4.761332
PRIMER_LEFT_264_PENALTY=2.359221
PRIMER_RIGHT_264_PENALTY=2.402111
PRIMER_LEFT_264_SEQUENCE=GCCCTTCTCGTTCCTGACA
PRIMER_RIGHT_264_SEQUENCE=TGTCTCTTTGGCGACGGT
PRIMER_LEFT_264=575,19
PRIMER_RIGHT_264=670,18
PRIMER_LEFT_264_TM=61.359
PRIMER_RIGHT_264_TM=60.402
PRIMER_LEFT_264_GC_PERCENT=57.895
PRIMER_RIGHT_264_GC_PERCENT=55.556
PRIMER_LEFT_264_SELF_ANY=3.00
PRIMER_RIGHT_264_SELF_ANY=3.00
PRIMER_LEFT_264_SELF_END=3.00
PRIMER_RIGHT_264_SELF_END=3.00
PRIMER_LEFT_264_END_STABILITY=6.7000
PRIMER_RIGHT_264_END_STABILITY=9.3000
PRIMER_PAIR_264_COMPL_ANY=4.00
PRIMER_PAIR_264_COMPL_END=1.00
PRIMER_PAIR_264_PRODUCT_SIZE=96
PRIMER_PAIR_264_PRODUCT_TM=74.2
PRIMER_PAIR_265_PENALTY=4.766661
PRIMER_LEFT_265_PENALTY=2.785923
PRIMER_RIGHT_265_PENALTY=1.980738
PRIMER_LEFT_265_SEQUENCE=CATGCACATGAATGTCCAGAAT
PRIMER_RIGHT_265_SEQUENCE=TCCTTGGTATCAATGCAGGT
PRIMER_LEFT_265=325,22
PRIMER_RIGHT_265=399,20
PRIMER_LEFT_265_TM=60.786
PRIMER_RIGHT_265_TM=58.019
PRIMER_LEFT_265_GC_PERCENT=40.909
PRIMER_RIGHT_265_GC_PERCENT=45.000
PRIMER_LEFT_265_SELF_ANY=8.00
PRIMER_RIGHT_265_SELF_ANY=6.00
PRIMER_LEFT_265_SELF_END=2.00
PRIMER_RIGHT_265_SELF_END=0.00
PRIMER_LEFT_265_END_STABILITY=6.6000
PRIMER_RIGHT_265_END_STABILITY=7.9000
PRIMER_PAIR_265_COMPL_ANY=4.00
PRIMER_PAIR_265_COMPL_END=2.00
PRIMER_PAIR_265_PRODUCT_SIZE=75
PRIMER_PAIR_265_PRODUCT_TM=71.0
PRIMER_PAIR_266_PENALTY=4.797870
PRIMER_LEFT_266_PENALTY=2.668729
PRIMER_RIGHT_266_PENALTY=2.129141
PRIMER_LEFT_266_SEQUENCE=ATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_266_SEQUENCE=GCACCAGTTCTGGATGGTCA
PRIMER_LEFT_266=414,21
PRIMER_RIGHT_266=493,20
PRIMER_LEFT_266_TM=58.331
PRIMER_RIGHT_266_TM=62.129
PRIMER_LEFT_266_GC_PERCENT=47.619
PRIMER_RIGHT_266_GC_PERCENT=55.000
PRIMER_LEFT_266_SELF_ANY=5.00
PRIMER_RIGHT_266_SELF_ANY=6.00
PRIMER_LEFT_266_SELF_END=1.00
PRIMER_RIGHT_266_SELF_END=2.00
PRIMER_LEFT_266_END_STABILITY=9.7000
PRIMER_RIGHT_266_END_STABILITY=7.9000
PRIMER_PAIR_266_COMPL_ANY=4.00
PRIMER_PAIR_266_COMPL_END=2.00
PRIMER_PAIR_266_PRODUCT_SIZE=80
PRIMER_PAIR_266_PRODUCT_TM=72.9
PRIMER_PAIR_267_PENALTY=4.805322
PRIMER_LEFT_267_PENALTY=1.014189
PRIMER_RIGHT_267_PENALTY=3.791133
PRIMER_LEFT_267_SEQUENCE=TCAGATCCATCAGGGACCA
PRIMER_RIGHT_267_SEQUENCE=CAGGGTAGACTTCTTGGCAATAC
PRIMER_LEFT_267=359,19
PRIMER_RIGHT_267=434,23
PRIMER_LEFT_267_TM=59.986
PRIMER_RIGHT_267_TM=59.209
PRIMER_LEFT_267_GC_PERCENT=52.632
PRIMER_RIGHT_267_GC_PERCENT=47.826
PRIMER_LEFT_267_SELF_ANY=5.00
PRIMER_RIGHT_267_SELF_ANY=5.00
PRIMER_LEFT_267_SELF_END=3.00
PRIMER_RIGHT_267_SELF_END=3.00
PRIMER_LEFT_267_END_STABILITY=7.9000
PRIMER_RIGHT_267_END_STABILITY=5.6000
PRIMER_PAIR_267_COMPL_ANY=3.00
PRIMER_PAIR_267_COMPL_END=0.00
PRIMER_PAIR_267_PRODUCT_SIZE=76
PRIMER_PAIR_267_PRODUCT_TM=72.0
PRIMER_PAIR_268_PENALTY=4.817718
PRIMER_LEFT_268_PENALTY=4.397076
PRIMER_RIGHT_268_PENALTY=0.420643
PRIMER_LEFT_268_SEQUENCE=CGGTTTGGCACTGCTCCT
PRIMER_RIGHT_268_SEQUENCE=AGGCCAGCATTACCATCAGT
PRIMER_LEFT_268=208,18
PRIMER_RIGHT_268=282,20
PRIMER_LEFT_268_TM=62.397
PRIMER_RIGHT_268_TM=59.579
PRIMER_LEFT_268_GC_PERCENT=61.111
PRIMER_RIGHT_268_GC_PERCENT=50.000
PRIMER_LEFT_268_SELF_ANY=5.00
PRIMER_RIGHT_268_SELF_ANY=4.00
PRIMER_LEFT_268_SELF_END=0.00
PRIMER_RIGHT_268_SELF_END=1.00
PRIMER_LEFT_268_END_STABILITY=7.9000
PRIMER_RIGHT_268_END_STABILITY=6.4000
PRIMER_PAIR_268_COMPL_ANY=6.00
PRIMER_PAIR_268_COMPL_END=3.00
PRIMER_PAIR_268_PRODUCT_SIZE=75
PRIMER_PAIR_268_PRODUCT_TM=79.2
PRIMER_PAIR_269_PENALTY=4.818613
PRIMER_LEFT_269_PENALTY=3.329319
PRIMER_RIGHT_269_PENALTY=1.489294
PRIMER_LEFT_269_SEQUENCE=CAAAGAGACATGCAGTGAGAAGA
PRIMER_RIGHT_269_SEQUENCE=GCAACACACAAACTCTACCCC
PRIMER_LEFT_269=661,23
PRIMER_RIGHT_269=760,21
PRIMER_LEFT_269_TM=59.671
PRIMER_RIGHT_269_TM=59.511
PRIMER_LEFT_269_GC_PERCENT=43.478
PRIMER_RIGHT_269_GC_PERCENT=52.381
PRIMER_LEFT_269_SELF_ANY=4.00
PRIMER_RIGHT_269_SELF_ANY=2.00
PRIMER_LEFT_269_SELF_END=0.00
PRIMER_RIGHT_269_SELF_END=0.00
PRIMER_LEFT_269_END_STABILITY=6.7000
PRIMER_RIGHT_269_END_STABILITY=10.6000
PRIMER_PAIR_269_COMPL_ANY=4.00
PRIMER_PAIR_269_COMPL_END=2.00
PRIMER_PAIR_269_PRODUCT_SIZE=100
PRIMER_PAIR_269_PRODUCT_TM=74.4
PRIMER_PAIR_270_PENALTY=4.849721
PRIMER_LEFT_270_PENALTY=3.200839
PRIMER_RIGHT_270_PENALTY=1.648882
PRIMER_LEFT_270_SEQUENCE=GCTGGCTGAACCCCAGAT
PRIMER_RIGHT_270_SEQUENCE=TGATGGATCTGAATCCCACTT
PRIMER_LEFT_270=283,18
PRIMER_RIGHT_270=370,21
PRIMER_LEFT_270_TM=61.201
PRIMER_RIGHT_270_TM=59.351
PRIMER_LEFT_270_GC_PERCENT=61.111
PRIMER_RIGHT_270_GC_PERCENT=42.857
PRIMER_LEFT_270_SELF_ANY=4.00
PRIMER_RIGHT_270_SELF_ANY=5.00
PRIMER_LEFT_270_SELF_END=2.00
PRIMER_RIGHT_270_SELF_END=0.00
PRIMER_LEFT_270_END_STABILITY=6.6000
PRIMER_RIGHT_270_END_STABILITY=6.7000
PRIMER_PAIR_270_COMPL_ANY=5.00
PRIMER_PAIR_270_COMPL_END=0.00
PRIMER_PAIR_270_PRODUCT_SIZE=88
PRIMER_PAIR_270_PRODUCT_TM=73.6
PRIMER_PAIR_271_PENALTY=4.881052
PRIMER_LEFT_271_PENALTY=1.081589
PRIMER_RIGHT_271_PENALTY=3.799463
PRIMER_LEFT_271_SEQUENCE=ATCAGGGACCAAAACCTGC
PRIMER_RIGHT_271_SEQUENCE=GCTTCTACCACATTGGTGATCT
PRIMER_LEFT_271=367,19
PRIMER_RIGHT_271=462,22
PRIMER_LEFT_271_TM=59.918
PRIMER_RIGHT_271_TM=58.201
PRIMER_LEFT_271_GC_PERCENT=52.632
PRIMER_RIGHT_271_GC_PERCENT=45.455
PRIMER_LEFT_271_SELF_ANY=4.00
PRIMER_RIGHT_271_SELF_ANY=6.00
PRIMER_LEFT_271_SELF_END=2.00
PRIMER_RIGHT_271_SELF_END=2.00
PRIMER_LEFT_271_END_STABILITY=9.7000
PRIMER_RIGHT_271_END_STABILITY=6.3000
PRIMER_PAIR_271_COMPL_ANY=5.00
PRIMER_PAIR_271_COMPL_END=0.00
PRIMER_PAIR_271_PRODUCT_SIZE=96
PRIMER_PAIR_271_PRODUCT_TM=73.3
PRIMER_PAIR_272_PENALTY=4.883797
PRIMER_LEFT_272_PENALTY=2.601441
PRIMER_RIGHT_272_PENALTY=2.282356
PRIMER_LEFT_272_SEQUENCE=GACCAAAACCTGCATTGATACC
PRIMER_RIGHT_272_SEQUENCE=GTTGGTTGGCTTCTACCACATT
PRIMER_LEFT_272=373,22
PRIMER_RIGHT_272=470,22
PRIMER_LEFT_272_TM=60.601
PRIMER_RIGHT_272_TM=60.282
PRIMER_LEFT_272_GC_PERCENT=45.455
PRIMER_RIGHT_272_GC_PERCENT=45.455
PRIMER_LEFT_272_SELF_ANY=4.00
PRIMER_RIGHT_272_SELF_ANY=4.00
PRIMER_LEFT_272_SELF_END=2.00
PRIMER_RIGHT_272_SELF_END=1.00
PRIMER_LEFT_272_END_STABILITY=6.8000
PRIMER_RIGHT_272_END_STABILITY=6.6000
PRIMER_PAIR_272_COMPL_ANY=5.00
PRIMER_PAIR_272_COMPL_END=1.00
PRIMER_PAIR_272_PRODUCT_SIZE=98
PRIMER_PAIR_272_PRODUCT_TM=73.4
PRIMER_PAIR_273_PENALTY=4.895033
PRIMER_LEFT_273_PENALTY=2.612614
PRIMER_RIGHT_273_PENALTY=2.282418
PRIMER_LEFT_273_SEQUENCE=CAAGACCCATCCCCACTTTG
PRIMER_RIGHT_273_SEQUENCE=GGAACGAGAAGGGCATCA
PRIMER_LEFT_273=514,20
PRIMER_RIGHT_273=588,18
PRIMER_LEFT_273_TM=62.613
PRIMER_RIGHT_273_TM=59.718
PRIMER_LEFT_273_GC_PERCENT=55.000
PRIMER_RIGHT_273_GC_PERCENT=55.556
PRIMER_LEFT_273_SELF_ANY=3.00
PRIMER_RIGHT_273_SELF_ANY=2.00
PRIMER_LEFT_273_SELF_END=1.00
PRIMER_RIGHT_273_SELF_END=1.00
PRIMER_LEFT_273_END_STABILITY=7.3000
PRIMER_RIGHT_273_END_STABILITY=6.9000
PRIMER_PAIR_273_COMPL_ANY=4.00
PRIMER_PAIR_273_COMPL_END=2.00
PRIMER_PAIR_273_PRODUCT_SIZE=75
PRIMER_PAIR_273_PRODUCT_TM=72.7
PRIMER_PAIR_274_PENALTY=4.900206
PRIMER_LEFT_274_PENALTY=2.653319
PRIMER_RIGHT_274_PENALTY=2.246886
PRIMER_LEFT_274_SEQUENCE=CCAATGTGGTAGAAGCCAACC
PRIMER_RIGHT_274_SEQUENCE=GGATGGGTCTTGCACTGCT
PRIMER_LEFT_274=447,21
PRIMER_RIGHT_274=525,19
PRIMER_LEFT_274_TM=61.653
PRIMER_RIGHT_274_TM=61.247
PRIMER_LEFT_274_GC_PERCENT=52.381
PRIMER_RIGHT_274_GC_PERCENT=57.895
PRIMER_LEFT_274_SELF_ANY=4.00
PRIMER_RIGHT_274_SELF_ANY=5.00
PRIMER_LEFT_274_SELF_END=2.00
PRIMER_RIGHT_274_SELF_END=3.00
PRIMER_LEFT_274_END_STABILITY=8.2000
PRIMER_RIGHT_274_END_STABILITY=8.2000
PRIMER_PAIR_274_COMPL_ANY=5.00
PRIMER_PAIR_274_COMPL_END=2.00
PRIMER_PAIR_274_PRODUCT_SIZE=79
PRIMER_PAIR_274_PRODUCT_TM=76.2
PRIMER_PAIR_275_PENALTY=4.913597
PRIMER_LEFT_275_PENALTY=3.239726
PRIMER_RIGHT_275_PENALTY=1.673871
PRIMER_LEFT_275_SEQUENCE=TGCATTGATACCAAGGAAGGC
PRIMER_RIGHT_275_SEQUENCE=TCACTGGTTGGTTGGCTTCTA
PRIMER_LEFT_275=383,21
PRIMER_RIGHT_275=476,21
PRIMER_LEFT_275_TM=62.240
PRIMER_RIGHT_275_TM=60.674
PRIMER_LEFT_275_GC_PERCENT=47.619
PRIMER_RIGHT_275_GC_PERCENT=47.619
PRIMER_LEFT_275_SELF_ANY=6.00
PRIMER_RIGHT_275_SELF_ANY=3.00
PRIMER_LEFT_275_SELF_END=2.00
PRIMER_RIGHT_275_SELF_END=2.00
PRIMER_LEFT_275_END_STABILITY=9.7000
PRIMER_RIGHT_275_END_STABILITY=6.0000
PRIMER_PAIR_275_COMPL_ANY=5.00
PRIMER_PAIR_275_COMPL_END=2.00
PRIMER_PAIR_275_PRODUCT_SIZE=94
PRIMER_PAIR_275_PRODUCT_TM=73.1
PRIMER_PAIR_276_PENALTY=4.919069
PRIMER_LEFT_276_PENALTY=2.487583
PRIMER_RIGHT_276_PENALTY=2.431486
PRIMER_LEFT_276_SEQUENCE=TTCTTACACCAGGAGAGGATGG
PRIMER_RIGHT_276_SEQUENCE=TTCTCACTGCATGTCTCTTTGG
PRIMER_LEFT_276=602,22
PRIMER_RIGHT_276=681,22
PRIMER_LEFT_276_TM=60.488
PRIMER_RIGHT_276_TM=60.431
PRIMER_LEFT_276_GC_PERCENT=50.000
PRIMER_RIGHT_276_GC_PERCENT=45.455
PRIMER_LEFT_276_SELF_ANY=3.00
PRIMER_RIGHT_276_SELF_ANY=4.00
PRIMER_LEFT_276_SELF_END=0.00
PRIMER_RIGHT_276_SELF_END=0.00
PRIMER_LEFT_276_END_STABILITY=8.1000
PRIMER_RIGHT_276_END_STABILITY=8.8000
PRIMER_PAIR_276_COMPL_ANY=5.00
PRIMER_PAIR_276_COMPL_END=3.00
PRIMER_PAIR_276_PRODUCT_SIZE=80
PRIMER_PAIR_276_PRODUCT_TM=72.4
PRIMER_PAIR_277_PENALTY=4.925479
PRIMER_LEFT_277_PENALTY=1.614650
PRIMER_RIGHT_277_PENALTY=3.310829
PRIMER_LEFT_277_SEQUENCE=ACCAGGAGAGGATGGATGTT
PRIMER_RIGHT_277_SEQUENCE=CCGTAGTCATGCAAGTTGG
PRIMER_LEFT_277=609,20
PRIMER_RIGHT_277=705,19
PRIMER_LEFT_277_TM=58.385
PRIMER_RIGHT_277_TM=57.689
PRIMER_LEFT_277_GC_PERCENT=50.000
PRIMER_RIGHT_277_GC_PERCENT=52.632
PRIMER_LEFT_277_SELF_ANY=3.00
PRIMER_RIGHT_277_SELF_ANY=5.00
PRIMER_LEFT_277_SELF_END=0.00
PRIMER_RIGHT_277_SELF_END=3.00
PRIMER_LEFT_277_END_STABILITY=6.6000
PRIMER_RIGHT_277_END_STABILITY=8.2000
PRIMER_PAIR_277_COMPL_ANY=4.00
PRIMER_PAIR_277_COMPL_END=0.00
PRIMER_PAIR_277_PRODUCT_SIZE=97
PRIMER_PAIR_277_PRODUCT_TM=74.4
PRIMER_PAIR_278_PENALTY=4.973196
PRIMER_LEFT_278_PENALTY=1.147302
PRIMER_RIGHT_278_PENALTY=3.825895
PRIMER_LEFT_278_SEQUENCE=GGAAGTGGGATTCAGATCCAT
PRIMER_RIGHT_278_SEQUENCE=TGCAGTTCAGGGTAGACTTCTTG
PRIMER_LEFT_278=348,21
PRIMER_RIGHT_278=441,23
PRIMER_LEFT_278_TM=60.147
PRIMER_RIGHT_278_TM=60.826
PRIMER_LEFT_278_GC_PERCENT=47.619
PRIMER_RIGHT_278_GC_PERCENT=47.826
PRIMER_LEFT_278_SELF_ANY=5.00
PRIMER_RIGHT_278_SELF_ANY=4.00
PRIMER_LEFT_278_SELF_END=2.00
PRIMER_RIGHT_278_SELF_END=1.00
PRIMER_LEFT_278_END_STABILITY=8.1000
PRIMER_RIGHT_278_END_STABILITY=7.0000
PRIMER_PAIR_278_COMPL_ANY=5.00
PRIMER_PAIR_278_COMPL_END=1.00
PRIMER_PAIR_278_PRODUCT_SIZE=94
PRIMER_PAIR_278_PRODUCT_TM=73.6
PRIMER_PAIR_279_PENALTY=4.998681
PRIMER_LEFT_279_PENALTY=2.459929
PRIMER_RIGHT_279_PENALTY=2.538752
PRIMER_LEFT_279_SEQUENCE=CCTACCGCTGCTTAGTTGG
PRIMER_RIGHT_279_SEQUENCE=ATCCATCCTCTCCTGGTGTAAG
PRIMER_LEFT_279=540,19
PRIMER_RIGHT_279=625,22
PRIMER_LEFT_279_TM=58.540
PRIMER_RIGHT_279_TM=59.461
PRIMER_LEFT_279_GC_PERCENT=57.895
PRIMER_RIGHT_279_GC_PERCENT=50.000
PRIMER_LEFT_279_SELF_ANY=3.00
PRIMER_RIGHT_279_SELF_ANY=3.00
PRIMER_LEFT_279_SELF_END=0.00
PRIMER_RIGHT_279_SELF_END=1.00
PRIMER_LEFT_279_END_STABILITY=8.2000
PRIMER_RIGHT_279_END_STABILITY=5.7000
PRIMER_PAIR_279_COMPL_ANY=4.00
PRIMER_PAIR_279_COMPL_END=1.00
PRIMER_PAIR_279_PRODUCT_SIZE=86
PRIMER_PAIR_279_PRODUCT_TM=72.5
PRIMER_PAIR_280_PENALTY=5.000320
PRIMER_LEFT_280_PENALTY=2.459929
PRIMER_RIGHT_280_PENALTY=2.540392
PRIMER_LEFT_280_SEQUENCE=CCCTACCGCTGCTTAGTTG
PRIMER_RIGHT_280_SEQUENCE=ATCCATCCTCTCCTGGTGTAA
PRIMER_LEFT_280=539,19
PRIMER_RIGHT_280=625,21
PRIMER_LEFT_280_TM=58.540
PRIMER_RIGHT_280_TM=58.460
PRIMER_LEFT_280_GC_PERCENT=57.895
PRIMER_RIGHT_280_GC_PERCENT=47.619
PRIMER_LEFT_280_SELF_ANY=3.00
PRIMER_RIGHT_280_SELF_ANY=3.00
PRIMER_LEFT_280_SELF_END=1.00
PRIMER_RIGHT_280_SELF_END=1.00
PRIMER_LEFT_280_END_STABILITY=6.7000
PRIMER_RIGHT_280_END_STABILITY=6.0000
PRIMER_PAIR_280_COMPL_ANY=3.00
PRIMER_PAIR_280_COMPL_END=1.00
PRIMER_PAIR_280_PRODUCT_SIZE=87
PRIMER_PAIR_280_PRODUCT_TM=72.8
PRIMER_PAIR_281_PENALTY=5.002464
PRIMER_LEFT_281_PENALTY=2.359221
PRIMER_RIGHT_281_PENALTY=2.643243
PRIMER_LEFT_281_SEQUENCE=TGCCCTTCTCGTTCCTGAC
PRIMER_RIGHT_281_SEQUENCE=TGTGCCAGTGAAGATGAGTTT
PRIMER_LEFT_281=574,19
PRIMER_RIGHT_281=653,21
PRIMER_LEFT_281_TM=61.359
PRIMER_RIGHT_281_TM=58.357
PRIMER_LEFT_281_GC_PERCENT=57.895
PRIMER_RIGHT_281_GC_PERCENT=42.857
PRIMER_LEFT_281_SELF_ANY=2.00
PRIMER_RIGHT_281_SELF_ANY=3.00
PRIMER_LEFT_281_SELF_END=1.00
PRIMER_RIGHT_281_SELF_END=1.00
PRIMER_LEFT_281_END_STABILITY=6.4000
PRIMER_RIGHT_281_END_STABILITY=6.7000
PRIMER_PAIR_281_COMPL_ANY=4.00
PRIMER_PAIR_281_COMPL_END=1.00
PRIMER_PAIR_281_PRODUCT_SIZE=80
PRIMER_PAIR_281_PRODUCT_TM=71.9
PRIMER_PAIR_282_PENALTY=5.018265
PRIMER_LEFT_282_PENALTY=1.604487
PRIMER_RIGHT_282_PENALTY=3.413779
PRIMER_LEFT_282_SEQUENCE=TGAATGTCCAGAATGGGAAGT
PRIMER_RIGHT_282_SEQUENCE=CAGGATGCCTTCCTTGGT
PRIMER_LEFT_282=333,21
PRIMER_RIGHT_282=409,18
PRIMER_LEFT_282_TM=59.396
PRIMER_RIGHT_282_TM=58.586
PRIMER_LEFT_282_GC_PERCENT=42.857
PRIMER_RIGHT_282_GC_PERCENT=55.556
PRIMER_LEFT_282_SELF_ANY=3.00
PRIMER_RIGHT_282_SELF_ANY=7.00
PRIMER_LEFT_282_SELF_END=1.00
PRIMER_RIGHT_282_SELF_END=0.00
PRIMER_LEFT_282_END_STABILITY=6.4000
PRIMER_RIGHT_282_END_STABILITY=8.2000
PRIMER_PAIR_282_COMPL_ANY=5.00
PRIMER_PAIR_282_COMPL_END=1.00
PRIMER_PAIR_282_PRODUCT_SIZE=77
PRIMER_PAIR_282_PRODUCT_TM=71.8
PRIMER_PAIR_283_PENALTY=5.045956
PRIMER_LEFT_283_PENALTY=2.895805
PRIMER_RIGHT_283_PENALTY=2.150151
PRIMER_LEFT_283_SEQUENCE=GGAGGTACCCACTGATGGTAAT
PRIMER_RIGHT_283_SEQUENCE=CTTCCCATTCTGGACATTCAT
PRIMER_LEFT_283=253,22
PRIMER_RIGHT_283=352,21
PRIMER_LEFT_283_TM=59.104
PRIMER_RIGHT_283_TM=58.850
PRIMER_LEFT_283_GC_PERCENT=50.000
PRIMER_RIGHT_283_GC_PERCENT=42.857
PRIMER_LEFT_283_SELF_ANY=6.00
PRIMER_RIGHT_283_SELF_ANY=3.00
PRIMER_LEFT_283_SELF_END=2.00
PRIMER_RIGHT_283_SELF_END=2.00
PRIMER_LEFT_283_END_STABILITY=5.6000
PRIMER_RIGHT_283_END_STABILITY=6.9000
PRIMER_PAIR_283_COMPL_ANY=5.00
PRIMER_PAIR_283_COMPL_END=2.00
PRIMER_PAIR_283_PRODUCT_SIZE=100
PRIMER_PAIR_283_PRODUCT_TM=75.6
PRIMER_PAIR_284_PENALTY=5.048065
PRIMER_LEFT_284_PENALTY=1.183296
PRIMER_RIGHT_284_PENALTY=3.864768
PRIMER_LEFT_284_SEQUENCE=AGATCCATCAGGGACCAAAAC
PRIMER_RIGHT_284_SEQUENCE=GCAGTTCAGGGTAGACTTCTTG
PRIMER_LEFT_284=361,21
PRIMER_RIGHT_284=440,22
PRIMER_LEFT_284_TM=60.183
PRIMER_RIGHT_284_TM=58.135
PRIMER_LEFT_284_GC_PERCENT=47.619
PRIMER_RIGHT_284_GC_PERCENT=50.000
PRIMER_LEFT_284_SELF_ANY=5.00
PRIMER_RIGHT_284_SELF_ANY=4.00
PRIMER_LEFT_284_SELF_END=0.00
PRIMER_RIGHT_284_SELF_END=1.00
PRIMER_LEFT_284_END_STABILITY=7.0000
PRIMER_RIGHT_284_END_STABILITY=7.0000
PRIMER_PAIR_284_COMPL_ANY=3.00
PRIMER_PAIR_284_COMPL_END=2.00
PRIMER_PAIR_284_PRODUCT_SIZE=80
PRIMER_PAIR_284_PRODUCT_TM=72.4
PRIMER_PAIR_285_PENALTY=5.057903
PRIMER_LEFT_285_PENALTY=2.752572
PRIMER_RIGHT_285_PENALTY=2.305331
PRIMER_LEFT_285_SEQUENCE=GATCACCAATGTGGTAGAAGCC
PRIMER_RIGHT_285_SEQUENCE=TCACAAAGTGGGGATGGG
PRIMER_LEFT_285=442,22
PRIMER_RIGHT_285=536,18
PRIMER_LEFT_285_TM=60.753
PRIMER_RIGHT_285_TM=60.305
PRIMER_LEFT_285_GC_PERCENT=50.000
PRIMER_RIGHT_285_GC_PERCENT=55.556
PRIMER_LEFT_285_SELF_ANY=6.00
PRIMER_RIGHT_285_SELF_ANY=3.00
PRIMER_LEFT_285_SELF_END=1.00
PRIMER_RIGHT_285_SELF_END=0.00
PRIMER_LEFT_285_END_STABILITY=9.7000
PRIMER_RIGHT_285_END_STABILITY=9.6000
PRIMER_PAIR_285_COMPL_ANY=6.00
PRIMER_PAIR_285_COMPL_END=2.00
PRIMER_PAIR_285_PRODUCT_SIZE=95
PRIMER_PAIR_285_PRODUCT_TM=76.5
PRIMER_PAIR_286_PENALTY=5.068898
PRIMER_LEFT_286_PENALTY=2.828479
PRIMER_RIGHT_286_PENALTY=2.240419
PRIMER_LEFT_286_SEQUENCE=AACCAACCAGTGACCATCC
PRIMER_RIGHT_286_SEQUENCE=ACTCACCAACTAAGCAGCGGT
PRIMER_LEFT_286=464,19
PRIMER_RIGHT_286=563,21
PRIMER_LEFT_286_TM=58.172
PRIMER_RIGHT_286_TM=61.240
PRIMER_LEFT_286_GC_PERCENT=52.632
PRIMER_RIGHT_286_GC_PERCENT=52.381
PRIMER_LEFT_286_SELF_ANY=3.00
PRIMER_RIGHT_286_SELF_ANY=3.00
PRIMER_LEFT_286_SELF_END=0.00
PRIMER_RIGHT_286_SELF_END=2.00
PRIMER_LEFT_286_END_STABILITY=8.1000
PRIMER_RIGHT_286_END_STABILITY=11.1000
PRIMER_PAIR_286_COMPL_ANY=4.00
PRIMER_PAIR_286_COMPL_END=1.00
PRIMER_PAIR_286_PRODUCT_SIZE=100
PRIMER_PAIR_286_PRODUCT_TM=76.9
PRIMER_PAIR_287_PENALTY=5.083942
PRIMER_LEFT_287_PENALTY=1.075611
PRIMER_RIGHT_287_PENALTY=4.008330
PRIMER_LEFT_287_SEQUENCE=CCAGAATGGGAAGTGGGATT
PRIMER_RIGHT_287_SEQUENCE=CAGTTCAGGGTAGACTTCTTGG
PRIMER_LEFT_287=340,20
PRIMER_RIGHT_287=439,22
PRIMER_LEFT_287_TM=61.076
PRIMER_RIGHT_287_TM=57.992
PRIMER_LEFT_287_GC_PERCENT=50.000
PRIMER_RIGHT_287_GC_PERCENT=50.000
PRIMER_LEFT_287_SELF_ANY=3.00
PRIMER_RIGHT_287_SELF_ANY=4.00
PRIMER_LEFT_287_SELF_END=1.00
PRIMER_RIGHT_287_SELF_END=0.00
PRIMER_LEFT_287_END_STABILITY=8.1000
PRIMER_RIGHT_287_END_STABILITY=8.5000
PRIMER_PAIR_287_COMPL_ANY=5.00
PRIMER_PAIR_287_COMPL_END=0.00
PRIMER_PAIR_287_PRODUCT_SIZE=100
PRIMER_PAIR_287_PRODUCT_TM=74.0
PRIMER_PAIR_288_PENALTY=5.100833
PRIMER_LEFT_288_PENALTY=1.236064
PRIMER_RIGHT_288_PENALTY=3.864768
PRIMER_LEFT_288_SEQUENCE=CCATCAGGGACCAAAACCT
PRIMER_RIGHT_288_SEQUENCE=TGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_288=365,19
PRIMER_RIGHT_288=441,22
PRIMER_LEFT_288_TM=59.764
PRIMER_RIGHT_288_TM=58.135
PRIMER_LEFT_288_GC_PERCENT=52.632
PRIMER_RIGHT_288_GC_PERCENT=45.455
PRIMER_LEFT_288_SELF_ANY=3.00
PRIMER_RIGHT_288_SELF_ANY=4.00
PRIMER_LEFT_288_SELF_END=0.00
PRIMER_RIGHT_288_SELF_END=2.00
PRIMER_LEFT_288_END_STABILITY=7.9000
PRIMER_RIGHT_288_END_STABILITY=7.0000
PRIMER_PAIR_288_COMPL_ANY=3.00
PRIMER_PAIR_288_COMPL_END=1.00
PRIMER_PAIR_288_PRODUCT_SIZE=77
PRIMER_PAIR_288_PRODUCT_TM=72.3
PRIMER_PAIR_289_PENALTY=5.123933
PRIMER_LEFT_289_PENALTY=3.216208
PRIMER_RIGHT_289_PENALTY=1.907724
PRIMER_LEFT_289_SEQUENCE=GTTCTGTGGCAGACTGAACAT
PRIMER_RIGHT_289_SEQUENCE=ATGCAGGTTTTGGTCCCTG
PRIMER_LEFT_289=307,21
PRIMER_RIGHT_289=387,19
PRIMER_LEFT_289_TM=57.784
PRIMER_RIGHT_289_TM=60.908
PRIMER_LEFT_289_GC_PERCENT=47.619
PRIMER_RIGHT_289_GC_PERCENT=52.632
PRIMER_LEFT_289_SELF_ANY=6.00
PRIMER_RIGHT_289_SELF_ANY=4.00
PRIMER_LEFT_289_SELF_END=3.00
PRIMER_RIGHT_289_SELF_END=1.00
PRIMER_LEFT_289_END_STABILITY=6.6000
PRIMER_RIGHT_289_END_STABILITY=9.7000
PRIMER_PAIR_289_COMPL_ANY=3.00
PRIMER_PAIR_289_COMPL_END=1.00
PRIMER_PAIR_289_PRODUCT_SIZE=81
PRIMER_PAIR_289_PRODUCT_TM=72.2
PRIMER_PAIR_290_PENALTY=5.165025
PRIMER_LEFT_290_PENALTY=1.938889
PRIMER_RIGHT_290_PENALTY=3.226137
PRIMER_LEFT_290_SEQUENCE=TTGCGAAACTCATCTTCACTG
PRIMER_RIGHT_290_SEQUENCE=GGCAGCAACATGCCGTAGT
PRIMER_LEFT_290=628,21
PRIMER_RIGHT_290=717,19
PRIMER_LEFT_290_TM=59.061
PRIMER_RIGHT_290_TM=62.226
PRIMER_LEFT_290_GC_PERCENT=42.857
PRIMER_RIGHT_290_GC_PERCENT=57.895
PRIMER_LEFT_290_SELF_ANY=4.00
PRIMER_RIGHT_290_SELF_ANY=4.00
PRIMER_LEFT_290_SELF_END=3.00
PRIMER_RIGHT_290_SELF_END=1.00
PRIMER_LEFT_290_END_STABILITY=6.7000
PRIMER_RIGHT_290_END_STABILITY=5.1000
PRIMER_PAIR_290_COMPL_ANY=4.00
PRIMER_PAIR_290_COMPL_END=2.00
PRIMER_PAIR_290_PRODUCT_SIZE=90
PRIMER_PAIR_290_PRODUCT_TM=74.2
PRIMER_PAIR_291_PENALTY=5.166383
PRIMER_LEFT_291_PENALTY=1.785441
PRIMER_RIGHT_291_PENALTY=3.380942
PRIMER_LEFT_291_SEQUENCE=ACACCAGGAGAGGATGGATGT
PRIMER_RIGHT_291_SEQUENCE=GCCGTAGTCATGCAAGTTGGT
PRIMER_LEFT_291=607,21
PRIMER_RIGHT_291=706,21
PRIMER_LEFT_291_TM=60.785
PRIMER_RIGHT_291_TM=62.381
PRIMER_LEFT_291_GC_PERCENT=52.381
PRIMER_RIGHT_291_GC_PERCENT=52.381
PRIMER_LEFT_291_SELF_ANY=4.00
PRIMER_RIGHT_291_SELF_ANY=5.00
PRIMER_LEFT_291_SELF_END=0.00
PRIMER_RIGHT_291_SELF_END=1.00
PRIMER_LEFT_291_END_STABILITY=6.3000
PRIMER_RIGHT_291_END_STABILITY=8.2000
PRIMER_PAIR_291_COMPL_ANY=4.00
PRIMER_PAIR_291_COMPL_END=0.00
PRIMER_PAIR_291_PRODUCT_SIZE=100
PRIMER_PAIR_291_PRODUCT_TM=74.8
PRIMER_PAIR_292_PENALTY=5.171362
PRIMER_LEFT_292_PENALTY=3.413779
PRIMER_RIGHT_292_PENALTY=1.757583
PRIMER_LEFT_292_SEQUENCE=CTGGCTGAACCCCAGATT
PRIMER_RIGHT_292_SEQUENCE=ATCTGAATCCCACTTCCCAT
PRIMER_LEFT_292=284,18
PRIMER_RIGHT_292=364,20
PRIMER_LEFT_292_TM=58.586
PRIMER_RIGHT_292_TM=58.242
PRIMER_LEFT_292_GC_PERCENT=55.556
PRIMER_RIGHT_292_GC_PERCENT=45.000
PRIMER_LEFT_292_SELF_ANY=4.00
PRIMER_RIGHT_292_SELF_ANY=3.00
PRIMER_LEFT_292_SELF_END=1.00
PRIMER_RIGHT_292_SELF_END=2.00
PRIMER_LEFT_292_END_STABILITY=6.6000
PRIMER_RIGHT_292_END_STABILITY=9.6000
PRIMER_PAIR_292_COMPL_ANY=5.00
PRIMER_PAIR_292_COMPL_END=1.00
PRIMER_PAIR_292_PRODUCT_SIZE=81
PRIMER_PAIR_292_PRODUCT_TM=72.7
PRIMER_PAIR_293_PENALTY=5.206692
PRIMER_LEFT_293_PENALTY=2.847471
PRIMER_RIGHT_293_PENALTY=2.359221
PRIMER_LEFT_293_SEQUENCE=ACCCATCCCCACTTTGTG
PRIMER_RIGHT_293_SEQUENCE=GTCAGGAACGAGAAGGGCA
PRIMER_LEFT_293=518,18
PRIMER_RIGHT_293=592,19
PRIMER_LEFT_293_TM=59.153
PRIMER_RIGHT_293_TM=61.359
PRIMER_LEFT_293_GC_PERCENT=55.556
PRIMER_RIGHT_293_GC_PERCENT=57.895
PRIMER_LEFT_293_SELF_ANY=3.00
PRIMER_RIGHT_293_SELF_ANY=2.00
PRIMER_LEFT_293_SELF_END=3.00
PRIMER_RIGHT_293_SELF_END=0.00
PRIMER_LEFT_293_END_STABILITY=7.0000
PRIMER_RIGHT_293_END_STABILITY=11.2000
PRIMER_PAIR_293_COMPL_ANY=4.00
PRIMER_PAIR_293_COMPL_END=2.00
PRIMER_PAIR_293_PRODUCT_SIZE=75
PRIMER_PAIR_293_PRODUCT_TM=72.7
PRIMER_PAIR_294_PENALTY=5.207392
PRIMER_LEFT_294_PENALTY=3.462244
PRIMER_RIGHT_294_PENALTY=1.745148
PRIMER_LEFT_294_SEQUENCE=CACACCGTCGCCAAAGAG
PRIMER_RIGHT_294_SEQUENCE=ACTCTACCCCTCGGAACTTG
PRIMER_LEFT_294=650,18
PRIMER_RIGHT_294=749,20
PRIMER_LEFT_294_TM=61.462
PRIMER_RIGHT_294_TM=58.255
PRIMER_LEFT_294_GC_PERCENT=61.111
PRIMER_RIGHT_294_GC_PERCENT=55.000
PRIMER_LEFT_294_SELF_ANY=3.00
PRIMER_RIGHT_294_SELF_ANY=3.00
PRIMER_LEFT_294_SELF_END=0.00
PRIMER_RIGHT_294_SELF_END=1.00
PRIMER_LEFT_294_END_STABILITY=6.7000
PRIMER_RIGHT_294_END_STABILITY=6.7000
PRIMER_PAIR_294_COMPL_ANY=4.00
PRIMER_PAIR_294_COMPL_END=0.00
PRIMER_PAIR_294_PRODUCT_SIZE=100
PRIMER_PAIR_294_PRODUCT_TM=75.6
PRIMER_PAIR_295_PENALTY=5.222637
PRIMER_LEFT_295_PENALTY=1.707711
PRIMER_RIGHT_295_PENALTY=3.514925
PRIMER_LEFT_295_SEQUENCE=CATGAATGTCCAGAATGGGAA
PRIMER_RIGHT_295_SEQUENCE=GCAGGATGCCTTCCTTGGT
PRIMER_LEFT_295=331,21
PRIMER_RIGHT_295=410,19
PRIMER_LEFT_295_TM=60.708
PRIMER_RIGHT_295_TM=62.515
PRIMER_LEFT_295_GC_PERCENT=42.857
PRIMER_RIGHT_295_GC_PERCENT=57.895
PRIMER_LEFT_295_SELF_ANY=4.00
PRIMER_RIGHT_295_SELF_ANY=7.00
PRIMER_LEFT_295_SELF_END=1.00
PRIMER_RIGHT_295_SELF_END=1.00
PRIMER_LEFT_295_END_STABILITY=9.7000
PRIMER_RIGHT_295_END_STABILITY=8.2000
PRIMER_PAIR_295_COMPL_ANY=4.00
PRIMER_PAIR_295_COMPL_END=1.00
PRIMER_PAIR_295_PRODUCT_SIZE=80
PRIMER_PAIR_295_PRODUCT_TM=72.4
PRIMER_PAIR_296_PENALTY=5.225323
PRIMER_LEFT_296_PENALTY=2.486287
PRIMER_RIGHT_296_PENALTY=2.739036
PRIMER_LEFT_296_SEQUENCE=TGCCCTTCTCGTTCCTGA
PRIMER_RIGHT_296_SEQUENCE=GTGTGCCAGTGAAGATGAGTTT
PRIMER_LEFT_296=574,18
PRIMER_RIGHT_296=654,22
PRIMER_LEFT_296_TM=60.486
PRIMER_RIGHT_296_TM=59.261
PRIMER_LEFT_296_GC_PERCENT=55.556
PRIMER_RIGHT_296_GC_PERCENT=45.455
PRIMER_LEFT_296_SELF_ANY=2.00
PRIMER_RIGHT_296_SELF_ANY=3.00
PRIMER_LEFT_296_SELF_END=2.00
PRIMER_RIGHT_296_SELF_END=1.00
PRIMER_LEFT_296_END_STABILITY=8.2000
PRIMER_RIGHT_296_END_STABILITY=6.7000
PRIMER_PAIR_296_COMPL_ANY=4.00
PRIMER_PAIR_296_COMPL_END=1.00
PRIMER_PAIR_296_PRODUCT_SIZE=81
PRIMER_PAIR_296_PRODUCT_TM=72.2
PRIMER_PAIR_297_PENALTY=5.246680
PRIMER_LEFT_297_PENALTY=1.357294
PRIMER_RIGHT_297_PENALTY=3.889386
PRIMER_LEFT_297_SEQUENCE=AATGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_297_SEQUENCE=CTGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_297=344,21
PRIMER_RIGHT_297=442,23
PRIMER_LEFT_297_TM=59.643
PRIMER_RIGHT_297_TM=59.111
PRIMER_LEFT_297_GC_PERCENT=42.857
PRIMER_RIGHT_297_GC_PERCENT=47.826
PRIMER_LEFT_297_SELF_ANY=3.00
PRIMER_RIGHT_297_SELF_ANY=6.00
PRIMER_LEFT_297_SELF_END=2.00
PRIMER_RIGHT_297_SELF_END=2.00
PRIMER_LEFT_297_END_STABILITY=6.6000
PRIMER_RIGHT_297_END_STABILITY=7.0000
PRIMER_PAIR_297_COMPL_ANY=5.00
PRIMER_PAIR_297_COMPL_END=1.00
PRIMER_PAIR_297_PRODUCT_SIZE=99
PRIMER_PAIR_297_PRODUCT_TM=73.7
PRIMER_PAIR_298_PENALTY=5.253631
PRIMER_LEFT_298_PENALTY=2.487598
PRIMER_RIGHT_298_PENALTY=2.766033
PRIMER_LEFT_298_SEQUENCE=GTGATTCCCTACCGCTGCTTA
PRIMER_RIGHT_298_SEQUENCE=ACATCCATCCTCTCCTGGTGTA
PRIMER_LEFT_298=533,21
PRIMER_RIGHT_298=627,22
PRIMER_LEFT_298_TM=61.488
PRIMER_RIGHT_298_TM=60.766
PRIMER_LEFT_298_GC_PERCENT=52.381
PRIMER_RIGHT_298_GC_PERCENT=50.000
PRIMER_LEFT_298_SELF_ANY=3.00
PRIMER_RIGHT_298_SELF_ANY=4.00
PRIMER_LEFT_298_SELF_END=2.00
PRIMER_RIGHT_298_SELF_END=2.00
PRIMER_LEFT_298_END_STABILITY=7.5000
PRIMER_RIGHT_298_END_STABILITY=5.4000
PRIMER_PAIR_298_COMPL_ANY=3.00
PRIMER_PAIR_298_COMPL_END=2.00
PRIMER_PAIR_298_PRODUCT_SIZE=95
PRIMER_PAIR_298_PRODUCT_TM=73.0
PRIMER_PAIR_299_PENALTY=5.287977
PRIMER_LEFT_299_PENALTY=1.818509
PRIMER_RIGHT_299_PENALTY=3.469469
PRIMER_LEFT_299_SEQUENCE=AGAGGATGGATGTTTGCGA
PRIMER_RIGHT_299_SEQUENCE=CCGTAGTCATGCAAGTTGGTACT
PRIMER_LEFT_299=615,19
PRIMER_RIGHT_299=705,23
PRIMER_LEFT_299_TM=59.181
PRIMER_RIGHT_299_TM=60.469
PRIMER_LEFT_299_GC_PERCENT=47.368
PRIMER_RIGHT_299_GC_PERCENT=47.826
PRIMER_LEFT_299_SELF_ANY=2.00
PRIMER_RIGHT_299_SELF_ANY=5.00
PRIMER_LEFT_299_SELF_END=2.00
PRIMER_RIGHT_299_SELF_END=3.00
PRIMER_LEFT_299_END_STABILITY=10.2000
PRIMER_RIGHT_299_END_STABILITY=5.1000
PRIMER_PAIR_299_COMPL_ANY=4.00
PRIMER_PAIR_299_COMPL_END=1.00
PRIMER_PAIR_299_PRODUCT_SIZE=91
PRIMER_PAIR_299_PRODUCT_TM=73.6
PRIMER_PAIR_300_PENALTY=5.293310
PRIMER_LEFT_300_PENALTY=2.384789
PRIMER_RIGHT_300_PENALTY=2.908522
PRIMER_LEFT_300_SEQUENCE=CTCGTTCCTGACAAGTGCAAA
PRIMER_RIGHT_300_SEQUENCE=GGTGTGCCAGTGAAGATGA
PRIMER_LEFT_300=581,21
PRIMER_RIGHT_300=655,19
PRIMER_LEFT_300_TM=61.385
PRIMER_RIGHT_300_TM=58.091
PRIMER_LEFT_300_GC_PERCENT=47.619
PRIMER_RIGHT_300_GC_PERCENT=52.632
PRIMER_LEFT_300_SELF_ANY=4.00
PRIMER_RIGHT_300_SELF_ANY=3.00
PRIMER_LEFT_300_SELF_END=0.00
PRIMER_RIGHT_300_SELF_END=1.00
PRIMER_LEFT_300_END_STABILITY=8.8000
PRIMER_RIGHT_300_END_STABILITY=6.6000
PRIMER_PAIR_300_COMPL_ANY=5.00
PRIMER_PAIR_300_COMPL_END=1.00
PRIMER_PAIR_300_PRODUCT_SIZE=75
PRIMER_PAIR_300_PRODUCT_TM=71.6
PRIMER_PAIR_301_PENALTY=5.306705
PRIMER_LEFT_301_PENALTY=3.269366
PRIMER_RIGHT_301_PENALTY=2.037339
PRIMER_LEFT_301_SEQUENCE=CATCCAGAACTGGTGCAAG
PRIMER_RIGHT_301_SEQUENCE=GGCATCACTTACAAACTCACCA
PRIMER_LEFT_301=478,19
PRIMER_RIGHT_301=577,22
PRIMER_LEFT_301_TM=57.731
PRIMER_RIGHT_301_TM=60.037
PRIMER_LEFT_301_GC_PERCENT=52.632
PRIMER_RIGHT_301_GC_PERCENT=45.455
PRIMER_LEFT_301_SELF_ANY=6.00
PRIMER_RIGHT_301_SELF_ANY=2.00
PRIMER_LEFT_301_SELF_END=2.00
PRIMER_RIGHT_301_SELF_END=0.00
PRIMER_LEFT_301_END_STABILITY=8.5000
PRIMER_RIGHT_301_END_STABILITY=8.2000
PRIMER_PAIR_301_COMPL_ANY=5.00
PRIMER_PAIR_301_COMPL_END=3.00
PRIMER_PAIR_301_PRODUCT_SIZE=100
PRIMER_PAIR_301_PRODUCT_TM=76.5
PRIMER_PAIR_302_PENALTY=5.324262
PRIMER_LEFT_302_PENALTY=3.279220
PRIMER_RIGHT_302_PENALTY=2.045042
PRIMER_LEFT_302_SEQUENCE=ACCCCAGATTGCCATGTT
PRIMER_RIGHT_302_SEQUENCE=CTGATGGATCTGAATCCCACT
PRIMER_LEFT_302=292,18
PRIMER_RIGHT_302=371,21
PRIMER_LEFT_302_TM=58.721
PRIMER_RIGHT_302_TM=58.955
PRIMER_LEFT_302_GC_PERCENT=50.000
PRIMER_RIGHT_302_GC_PERCENT=47.619
PRIMER_LEFT_302_SELF_ANY=4.00
PRIMER_RIGHT_302_SELF_ANY=5.00
PRIMER_LEFT_302_SELF_END=2.00
PRIMER_RIGHT_302_SELF_END=1.00
PRIMER_LEFT_302_END_STABILITY=6.6000
PRIMER_RIGHT_302_END_STABILITY=7.9000
PRIMER_PAIR_302_COMPL_ANY=5.00
PRIMER_PAIR_302_COMPL_END=0.00
PRIMER_PAIR_302_PRODUCT_SIZE=80
PRIMER_PAIR_302_PRODUCT_TM=72.4
PRIMER_PAIR_303_PENALTY=5.329358
PRIMER_LEFT_303_PENALTY=3.291759
PRIMER_RIGHT_303_PENALTY=2.037599
PRIMER_LEFT_303_SEQUENCE=CCATGTTCTGTGGCAGACTGA
PRIMER_RIGHT_303_SEQUENCE=CCTTGGTATCAATGCAGGTTT
PRIMER_LEFT_303=303,21
PRIMER_RIGHT_303=398,21
PRIMER_LEFT_303_TM=62.292
PRIMER_RIGHT_303_TM=58.962
PRIMER_LEFT_303_GC_PERCENT=52.381
PRIMER_RIGHT_303_GC_PERCENT=42.857
PRIMER_LEFT_303_SELF_ANY=5.00
PRIMER_RIGHT_303_SELF_ANY=6.00
PRIMER_LEFT_303_SELF_END=3.00
PRIMER_RIGHT_303_SELF_END=1.00
PRIMER_LEFT_303_END_STABILITY=6.4000
PRIMER_RIGHT_303_END_STABILITY=8.2000
PRIMER_PAIR_303_COMPL_ANY=3.00
PRIMER_PAIR_303_COMPL_END=1.00
PRIMER_PAIR_303_PRODUCT_SIZE=96
PRIMER_PAIR_303_PRODUCT_TM=73.3
PRIMER_PAIR_304_PENALTY=5.352938
PRIMER_LEFT_304_PENALTY=3.571483
PRIMER_RIGHT_304_PENALTY=1.781455
PRIMER_LEFT_304_SEQUENCE=CCTGCAGTATTGCCAAGAA
PRIMER_RIGHT_304_SEQUENCE=ATGGTCACTGGTTGGTTGG
PRIMER_LEFT_304=406,19
PRIMER_RIGHT_304=480,19
PRIMER_LEFT_304_TM=57.429
PRIMER_RIGHT_304_TM=59.219
PRIMER_LEFT_304_GC_PERCENT=47.368
PRIMER_RIGHT_304_GC_PERCENT=52.632
PRIMER_LEFT_304_SELF_ANY=6.00
PRIMER_RIGHT_304_SELF_ANY=3.00
PRIMER_LEFT_304_SELF_END=3.00
PRIMER_RIGHT_304_SELF_END=0.00
PRIMER_LEFT_304_END_STABILITY=7.0000
PRIMER_RIGHT_304_END_STABILITY=8.2000
PRIMER_PAIR_304_COMPL_ANY=4.00
PRIMER_PAIR_304_COMPL_END=3.00
PRIMER_PAIR_304_PRODUCT_SIZE=75
PRIMER_PAIR_304_PRODUCT_TM=72.1
PRIMER_PAIR_305_PENALTY=5.354170
PRIMER_LEFT_305_PENALTY=1.278124
PRIMER_RIGHT_305_PENALTY=4.076046
PRIMER_LEFT_305_SEQUENCE=CCAGAATGGGAAGTGGGAT
PRIMER_RIGHT_305_SEQUENCE=CAGGGTAGACTTCTTGGCAATACT
PRIMER_LEFT_305=340,19
PRIMER_RIGHT_305=434,24
PRIMER_LEFT_305_TM=59.722
PRIMER_RIGHT_305_TM=60.076
PRIMER_LEFT_305_GC_PERCENT=52.632
PRIMER_RIGHT_305_GC_PERCENT=45.833
PRIMER_LEFT_305_SELF_ANY=3.00
PRIMER_RIGHT_305_SELF_ANY=5.00
PRIMER_LEFT_305_SELF_END=2.00
PRIMER_RIGHT_305_SELF_END=2.00
PRIMER_LEFT_305_END_STABILITY=9.3000
PRIMER_RIGHT_305_END_STABILITY=5.3000
PRIMER_PAIR_305_COMPL_ANY=5.00
PRIMER_PAIR_305_COMPL_END=1.00
PRIMER_PAIR_305_PRODUCT_SIZE=95
PRIMER_PAIR_305_PRODUCT_TM=73.9
PRIMER_PAIR_306_PENALTY=5.364772
PRIMER_LEFT_306_PENALTY=1.798843
PRIMER_RIGHT_306_PENALTY=3.565929
PRIMER_LEFT_306_SEQUENCE=ACATGAATGTCCAGAATGGG
PRIMER_RIGHT_306_SEQUENCE=GCAGGATGCCTTCCTTGGTAT
PRIMER_LEFT_306=330,20
PRIMER_RIGHT_306=410,21
PRIMER_LEFT_306_TM=58.201
PRIMER_RIGHT_306_TM=62.566
PRIMER_LEFT_306_GC_PERCENT=45.000
PRIMER_RIGHT_306_GC_PERCENT=52.381
PRIMER_LEFT_306_SELF_ANY=6.00
PRIMER_RIGHT_306_SELF_ANY=7.00
PRIMER_LEFT_306_SELF_END=1.00
PRIMER_RIGHT_306_SELF_END=2.00
PRIMER_LEFT_306_END_STABILITY=9.6000
PRIMER_RIGHT_306_END_STABILITY=6.8000
PRIMER_PAIR_306_COMPL_ANY=3.00
PRIMER_PAIR_306_COMPL_END=2.00
PRIMER_PAIR_306_PRODUCT_SIZE=81
PRIMER_PAIR_306_PRODUCT_TM=72.2
PRIMER_PAIR_307_PENALTY=5.380369
PRIMER_LEFT_307_PENALTY=2.623229
PRIMER_RIGHT_307_PENALTY=2.757140
PRIMER_LEFT_307_SEQUENCE=TTCCCTACCGCTGCTTAGTTG
PRIMER_RIGHT_307_SEQUENCE=GTTTCGCAAACATCCATCCTC
PRIMER_LEFT_307=537,21
PRIMER_RIGHT_307=636,21
PRIMER_LEFT_307_TM=61.623
PRIMER_RIGHT_307_TM=61.757
PRIMER_LEFT_307_GC_PERCENT=52.381
PRIMER_RIGHT_307_GC_PERCENT=47.619
PRIMER_LEFT_307_SELF_ANY=3.00
PRIMER_RIGHT_307_SELF_ANY=6.00
PRIMER_LEFT_307_SELF_END=1.00
PRIMER_RIGHT_307_SELF_END=0.00
PRIMER_LEFT_307_END_STABILITY=6.7000
PRIMER_RIGHT_307_END_STABILITY=7.9000
PRIMER_PAIR_307_COMPL_ANY=3.00
PRIMER_PAIR_307_COMPL_END=1.00
PRIMER_PAIR_307_PRODUCT_SIZE=100
PRIMER_PAIR_307_PRODUCT_TM=73.2
PRIMER_PAIR_308_PENALTY=5.384900
PRIMER_LEFT_308_PENALTY=1.818509
PRIMER_RIGHT_308_PENALTY=3.566391
PRIMER_LEFT_308_SEQUENCE=GAGAGGATGGATGTTTGCG
PRIMER_RIGHT_308_SEQUENCE=CATGCAAGTTGGTACTCTTCTCA
PRIMER_LEFT_308=614,19
PRIMER_RIGHT_308=698,23
PRIMER_LEFT_308_TM=59.181
PRIMER_RIGHT_308_TM=59.434
PRIMER_LEFT_308_GC_PERCENT=52.632
PRIMER_RIGHT_308_GC_PERCENT=43.478
PRIMER_LEFT_308_SELF_ANY=2.00
PRIMER_RIGHT_308_SELF_ANY=5.00
PRIMER_LEFT_308_SELF_END=2.00
PRIMER_RIGHT_308_SELF_END=1.00
PRIMER_LEFT_308_END_STABILITY=10.5000
PRIMER_RIGHT_308_END_STABILITY=6.7000
PRIMER_PAIR_308_COMPL_ANY=5.00
PRIMER_PAIR_308_COMPL_END=2.00
PRIMER_PAIR_308_PRODUCT_SIZE=85
PRIMER_PAIR_308_PRODUCT_TM=73.1
PRIMER_PAIR_309_PENALTY=5.388009
PRIMER_LEFT_309_PENALTY=2.901722
PRIMER_RIGHT_309_PENALTY=2.486287
PRIMER_LEFT_309_SEQUENCE=GCAAGACCCATCCCCACT
PRIMER_RIGHT_309_SEQUENCE=TCAGGAACGAGAAGGGCA
PRIMER_LEFT_309=513,18
PRIMER_RIGHT_309=591,18
PRIMER_LEFT_309_TM=60.902
PRIMER_RIGHT_309_TM=60.486
PRIMER_LEFT_309_GC_PERCENT=61.111
PRIMER_RIGHT_309_GC_PERCENT=55.556
PRIMER_LEFT_309_SELF_ANY=2.00
PRIMER_RIGHT_309_SELF_ANY=2.00
PRIMER_LEFT_309_SELF_END=1.00
PRIMER_RIGHT_309_SELF_END=0.00
PRIMER_LEFT_309_END_STABILITY=7.9000
PRIMER_RIGHT_309_END_STABILITY=11.2000
PRIMER_PAIR_309_COMPL_ANY=4.00
PRIMER_PAIR_309_COMPL_END=2.00
PRIMER_PAIR_309_PRODUCT_SIZE=79
PRIMER_PAIR_309_PRODUCT_TM=73.1
PRIMER_PAIR_310_PENALTY=5.390157
PRIMER_LEFT_310_PENALTY=2.601481
PRIMER_RIGHT_310_PENALTY=2.788676
PRIMER_LEFT_310_SEQUENCE=TGATTCCCTACCGCTGCTTAG
PRIMER_RIGHT_310_SEQUENCE=CAAACATCCATCCTCTCCTGG
PRIMER_LEFT_310=534,21
PRIMER_RIGHT_310=630,21
PRIMER_LEFT_310_TM=61.601
PRIMER_RIGHT_310_TM=61.789
PRIMER_LEFT_310_GC_PERCENT=52.381
PRIMER_RIGHT_310_GC_PERCENT=52.381
PRIMER_LEFT_310_SELF_ANY=3.00
PRIMER_RIGHT_310_SELF_ANY=3.00
PRIMER_LEFT_310_SELF_END=3.00
PRIMER_RIGHT_310_SELF_END=3.00
PRIMER_LEFT_310_END_STABILITY=6.0000
PRIMER_RIGHT_310_END_STABILITY=9.7000
PRIMER_PAIR_310_COMPL_ANY=3.00
PRIMER_PAIR_310_COMPL_END=0.00
PRIMER_PAIR_310_PRODUCT_SIZE=97
PRIMER_PAIR_310_PRODUCT_TM=72.7
PRIMER_PAIR_311_PENALTY=5.419477
PRIMER_LEFT_311_PENALTY=2.933397
PRIMER_RIGHT_311_PENALTY=2.486079
PRIMER_LEFT_311_SEQUENCE=AACTGCAGATCACCAATGTG
PRIMER_RIGHT_311_SEQUENCE=TGGGTCTTGCACTGCTTG
PRIMER_LEFT_311=435,20
PRIMER_RIGHT_311=522,18
PRIMER_LEFT_311_TM=57.067
PRIMER_RIGHT_311_TM=59.514
PRIMER_LEFT_311_GC_PERCENT=45.000
PRIMER_RIGHT_311_GC_PERCENT=55.556
PRIMER_LEFT_311_SELF_ANY=6.00
PRIMER_RIGHT_311_SELF_ANY=5.00
PRIMER_LEFT_311_SELF_END=3.00
PRIMER_RIGHT_311_SELF_END=3.00
PRIMER_LEFT_311_END_STABILITY=6.6000
PRIMER_RIGHT_311_END_STABILITY=8.5000
PRIMER_PAIR_311_COMPL_ANY=4.00
PRIMER_PAIR_311_COMPL_END=1.00
PRIMER_PAIR_311_PRODUCT_SIZE=88
PRIMER_PAIR_311_PRODUCT_TM=75.9
PRIMER_PAIR_312_PENALTY=5.444251
PRIMER_LEFT_312_PENALTY=3.937317
PRIMER_RIGHT_312_PENALTY=1.506934
PRIMER_LEFT_312_SEQUENCE=CGTCGCCAAAGAGACATGC
PRIMER_RIGHT_312_SEQUENCE=ACAAACTCTACCCCTCGGAAC
PRIMER_LEFT_312=655,19
PRIMER_RIGHT_312=753,21
PRIMER_LEFT_312_TM=62.937
PRIMER_RIGHT_312_TM=59.493
PRIMER_LEFT_312_GC_PERCENT=57.895
PRIMER_RIGHT_312_GC_PERCENT=52.381
PRIMER_LEFT_312_SELF_ANY=4.00
PRIMER_RIGHT_312_SELF_ANY=3.00
PRIMER_LEFT_312_SELF_END=2.00
PRIMER_RIGHT_312_SELF_END=0.00
PRIMER_LEFT_312_END_STABILITY=8.4000
PRIMER_RIGHT_312_END_STABILITY=7.9000
PRIMER_PAIR_312_COMPL_ANY=4.00
PRIMER_PAIR_312_COMPL_END=0.00
PRIMER_PAIR_312_PRODUCT_SIZE=99
PRIMER_PAIR_312_PRODUCT_TM=75.0
PRIMER_PAIR_313_PENALTY=5.459430
PRIMER_LEFT_313_PENALTY=2.813295
PRIMER_RIGHT_313_PENALTY=2.646135
PRIMER_LEFT_313_SEQUENCE=CCATCCCCACTTTGTGATT
PRIMER_RIGHT_313_SEQUENCE=TTGTCAGGAACGAGAAGGGC
PRIMER_LEFT_313=520,19
PRIMER_RIGHT_313=594,20
PRIMER_LEFT_313_TM=58.187
PRIMER_RIGHT_313_TM=62.646
PRIMER_LEFT_313_GC_PERCENT=47.368
PRIMER_RIGHT_313_GC_PERCENT=55.000
PRIMER_LEFT_313_SELF_ANY=4.00
PRIMER_RIGHT_313_SELF_ANY=3.00
PRIMER_LEFT_313_SELF_END=2.00
PRIMER_RIGHT_313_SELF_END=2.00
PRIMER_LEFT_313_END_STABILITY=6.9000
PRIMER_RIGHT_313_END_STABILITY=10.9000
PRIMER_PAIR_313_COMPL_ANY=3.00
PRIMER_PAIR_313_COMPL_END=0.00
PRIMER_PAIR_313_PRODUCT_SIZE=75
PRIMER_PAIR_313_PRODUCT_TM=72.1
PRIMER_PAIR_314_PENALTY=5.467190
PRIMER_LEFT_314_PENALTY=2.710050
PRIMER_RIGHT_314_PENALTY=2.757140
PRIMER_LEFT_314_SEQUENCE=CCGCTGCTTAGTTGGTGAGTT
PRIMER_RIGHT_314_SEQUENCE=GAGTTTCGCAAACATCCATCC
PRIMER_LEFT_314=544,21
PRIMER_RIGHT_314=638,21
PRIMER_LEFT_314_TM=61.710
PRIMER_RIGHT_314_TM=61.757
PRIMER_LEFT_314_GC_PERCENT=52.381
PRIMER_RIGHT_314_GC_PERCENT=47.619
PRIMER_LEFT_314_SELF_ANY=3.00
PRIMER_RIGHT_314_SELF_ANY=6.00
PRIMER_LEFT_314_SELF_END=0.00
PRIMER_RIGHT_314_SELF_END=0.00
PRIMER_LEFT_314_END_STABILITY=6.4000
PRIMER_RIGHT_314_END_STABILITY=8.1000
PRIMER_PAIR_314_COMPL_ANY=4.00
PRIMER_PAIR_314_COMPL_END=2.00
PRIMER_PAIR_314_PRODUCT_SIZE=95
PRIMER_PAIR_314_PRODUCT_TM=73.0
PRIMER_PAIR_315_PENALTY=5.473658
PRIMER_LEFT_315_PENALTY=2.640342
PRIMER_RIGHT_315_PENALTY=2.833316
PRIMER_LEFT_315_SEQUENCE=CCACTTTGTGATTCCCTACCG
PRIMER_RIGHT_315_SEQUENCE=CCATCCTCTCCTGGTGTAAG
PRIMER_LEFT_315=526,21
PRIMER_RIGHT_315=623,20
PRIMER_LEFT_315_TM=61.640
PRIMER_RIGHT_315_TM=57.167
PRIMER_LEFT_315_GC_PERCENT=52.381
PRIMER_RIGHT_315_GC_PERCENT=55.000
PRIMER_LEFT_315_SELF_ANY=3.00
PRIMER_RIGHT_315_SELF_ANY=3.00
PRIMER_LEFT_315_SELF_END=2.00
PRIMER_RIGHT_315_SELF_END=1.00
PRIMER_LEFT_315_END_STABILITY=8.9000
PRIMER_RIGHT_315_END_STABILITY=5.7000
PRIMER_PAIR_315_COMPL_ANY=3.00
PRIMER_PAIR_315_COMPL_END=1.00
PRIMER_PAIR_315_PRODUCT_SIZE=98
PRIMER_PAIR_315_PRODUCT_TM=73.4
PRIMER_PAIR_316_PENALTY=5.481415
PRIMER_LEFT_316_PENALTY=3.352274
PRIMER_RIGHT_316_PENALTY=2.129141
PRIMER_LEFT_316_SEQUENCE=CTGCAGTATTGCCAAGAAGTC
PRIMER_RIGHT_316_SEQUENCE=TGCACCAGTTCTGGATGGTC
PRIMER_LEFT_316=407,21
PRIMER_RIGHT_316=494,20
PRIMER_LEFT_316_TM=57.648
PRIMER_RIGHT_316_TM=62.129
PRIMER_LEFT_316_GC_PERCENT=47.619
PRIMER_RIGHT_316_GC_PERCENT=55.000
PRIMER_LEFT_316_SELF_ANY=6.00
PRIMER_RIGHT_316_SELF_ANY=6.00
PRIMER_LEFT_316_SELF_END=2.00
PRIMER_RIGHT_316_SELF_END=3.00
PRIMER_LEFT_316_END_STABILITY=6.4000
PRIMER_RIGHT_316_END_STABILITY=7.9000
PRIMER_PAIR_316_COMPL_ANY=4.00
PRIMER_PAIR_316_COMPL_END=1.00
PRIMER_PAIR_316_PRODUCT_SIZE=88
PRIMER_PAIR_316_PRODUCT_TM=73.6
PRIMER_PAIR_317_PENALTY=5.496047
PRIMER_LEFT_317_PENALTY=3.377310
PRIMER_RIGHT_317_PENALTY=2.118737
PRIMER_LEFT_317_SEQUENCE=CACTGATGGTAATGCTGGC
PRIMER_RIGHT_317_SEQUENCE=CCACTTCCCATTCTGGACATT
PRIMER_LEFT_317=262,19
PRIMER_RIGHT_317=355,21
PRIMER_LEFT_317_TM=57.623
PRIMER_RIGHT_317_TM=61.119
PRIMER_LEFT_317_GC_PERCENT=52.632
PRIMER_RIGHT_317_GC_PERCENT=47.619
PRIMER_LEFT_317_SELF_ANY=3.00
PRIMER_RIGHT_317_SELF_ANY=3.00
PRIMER_LEFT_317_SELF_END=2.00
PRIMER_RIGHT_317_SELF_END=1.00
PRIMER_LEFT_317_END_STABILITY=9.7000
PRIMER_RIGHT_317_END_STABILITY=6.6000
PRIMER_PAIR_317_COMPL_ANY=5.00
PRIMER_PAIR_317_COMPL_END=1.00
PRIMER_PAIR_317_PRODUCT_SIZE=94
PRIMER_PAIR_317_PRODUCT_TM=74.9
PRIMER_PAIR_318_PENALTY=5.505184
PRIMER_LEFT_318_PENALTY=1.440351
PRIMER_RIGHT_318_PENALTY=4.064833
PRIMER_LEFT_318_SEQUENCE=TCAGATCCATCAGGGACCAA
PRIMER_RIGHT_318_SEQUENCE=ATCTGCAGTTCAGGGTAGACTTC
PRIMER_LEFT_318=359,20
PRIMER_RIGHT_318=444,23
PRIMER_LEFT_318_TM=61.440
PRIMER_RIGHT_318_TM=58.935
PRIMER_LEFT_318_GC_PERCENT=50.000
PRIMER_RIGHT_318_GC_PERCENT=47.826
PRIMER_LEFT_318_SELF_ANY=5.00
PRIMER_RIGHT_318_SELF_ANY=6.00
PRIMER_LEFT_318_SELF_END=1.00
PRIMER_RIGHT_318_SELF_END=3.00
PRIMER_LEFT_318_END_STABILITY=8.2000
PRIMER_RIGHT_318_END_STABILITY=6.4000
PRIMER_PAIR_318_COMPL_ANY=5.00
PRIMER_PAIR_318_COMPL_END=1.00
PRIMER_PAIR_318_PRODUCT_SIZE=86
PRIMER_PAIR_318_PRODUCT_TM=72.5
PRIMER_PAIR_319_PENALTY=5.505184
PRIMER_LEFT_319_PENALTY=1.440351
PRIMER_RIGHT_319_PENALTY=4.064833
PRIMER_LEFT_319_SEQUENCE=TTCAGATCCATCAGGGACCA
PRIMER_RIGHT_319_SEQUENCE=GATCTGCAGTTCAGGGTAGACTT
PRIMER_LEFT_319=358,20
PRIMER_RIGHT_319=445,23
PRIMER_LEFT_319_TM=61.440
PRIMER_RIGHT_319_TM=58.935
PRIMER_LEFT_319_GC_PERCENT=50.000
PRIMER_RIGHT_319_GC_PERCENT=47.826
PRIMER_LEFT_319_SELF_ANY=5.00
PRIMER_RIGHT_319_SELF_ANY=8.00
PRIMER_LEFT_319_SELF_END=3.00
PRIMER_RIGHT_319_SELF_END=1.00
PRIMER_LEFT_319_END_STABILITY=7.9000
PRIMER_RIGHT_319_END_STABILITY=6.4000
PRIMER_PAIR_319_COMPL_ANY=6.00
PRIMER_PAIR_319_COMPL_END=1.00
PRIMER_PAIR_319_PRODUCT_SIZE=88
PRIMER_PAIR_319_PRODUCT_TM=72.7
PRIMER_PAIR_320_PENALTY=5.506140
PRIMER_LEFT_320_PENALTY=3.449712
PRIMER_RIGHT_320_PENALTY=2.056428
PRIMER_LEFT_320_SEQUENCE=TCTGTGGCAGACTGAACATGC
PRIMER_RIGHT_320_SEQUENCE=GGTATCAATGCAGGTTTTGG
PRIMER_LEFT_320=309,21
PRIMER_RIGHT_320=394,20
PRIMER_LEFT_320_TM=62.450
PRIMER_RIGHT_320_TM=57.944
PRIMER_LEFT_320_GC_PERCENT=52.381
PRIMER_RIGHT_320_GC_PERCENT=45.000
PRIMER_LEFT_320_SELF_ANY=5.00
PRIMER_RIGHT_320_SELF_ANY=4.00
PRIMER_LEFT_320_SELF_END=2.00
PRIMER_RIGHT_320_SELF_END=0.00
PRIMER_LEFT_320_END_STABILITY=8.4000
PRIMER_RIGHT_320_END_STABILITY=8.8000
PRIMER_PAIR_320_COMPL_ANY=5.00
PRIMER_PAIR_320_COMPL_END=3.00
PRIMER_PAIR_320_PRODUCT_SIZE=86
PRIMER_PAIR_320_PRODUCT_TM=72.5
PRIMER_PAIR_321_PENALTY=5.507375
PRIMER_LEFT_321_PENALTY=2.785923
PRIMER_RIGHT_321_PENALTY=2.721451
PRIMER_LEFT_321_SEQUENCE=ATGCACATGAATGTCCAGAATG
PRIMER_RIGHT_321_SEQUENCE=TTCCTTGGTATCAATGCAGGTT
PRIMER_LEFT_321=326,22
PRIMER_RIGHT_321=400,22
PRIMER_LEFT_321_TM=60.786
PRIMER_RIGHT_321_TM=60.721
PRIMER_LEFT_321_GC_PERCENT=40.909
PRIMER_RIGHT_321_GC_PERCENT=40.909
PRIMER_LEFT_321_SELF_ANY=8.00
PRIMER_RIGHT_321_SELF_ANY=6.00
PRIMER_LEFT_321_SELF_END=1.00
PRIMER_RIGHT_321_SELF_END=0.00
PRIMER_LEFT_321_END_STABILITY=6.9000
PRIMER_RIGHT_321_END_STABILITY=7.9000
PRIMER_PAIR_321_COMPL_ANY=4.00
PRIMER_PAIR_321_COMPL_END=1.00
PRIMER_PAIR_321_PRODUCT_SIZE=75
PRIMER_PAIR_321_PRODUCT_TM=70.5
PRIMER_PAIR_322_PENALTY=5.513615
PRIMER_LEFT_322_PENALTY=1.928663
PRIMER_RIGHT_322_PENALTY=3.584952
PRIMER_LEFT_322_SEQUENCE=CAGGAGAGGATGGATGTTTG
PRIMER_RIGHT_322_SEQUENCE=TGGTACTCTTCTCACTGCATGTC
PRIMER_LEFT_322=611,20
PRIMER_RIGHT_322=689,23
PRIMER_LEFT_322_TM=58.071
PRIMER_RIGHT_322_TM=59.415
PRIMER_LEFT_322_GC_PERCENT=50.000
PRIMER_RIGHT_322_GC_PERCENT=47.826
PRIMER_LEFT_322_SELF_ANY=2.00
PRIMER_RIGHT_322_SELF_ANY=4.00
PRIMER_LEFT_322_SELF_END=0.00
PRIMER_RIGHT_322_SELF_END=2.00
PRIMER_LEFT_322_END_STABILITY=7.0000
PRIMER_RIGHT_322_END_STABILITY=6.3000
PRIMER_PAIR_322_COMPL_ANY=6.00
PRIMER_PAIR_322_COMPL_END=1.00
PRIMER_PAIR_322_PRODUCT_SIZE=79
PRIMER_PAIR_322_PRODUCT_TM=73.1
PRIMER_PAIR_323_PENALTY=5.532363
PRIMER_LEFT_323_PENALTY=2.652125
PRIMER_RIGHT_323_PENALTY=2.880239
PRIMER_LEFT_323_SEQUENCE=TGATTCCCTACCGCTGCT
PRIMER_RIGHT_323_SEQUENCE=CGCAAACATCCATCCTCTCC
PRIMER_LEFT_323=534,18
PRIMER_RIGHT_323=632,20
PRIMER_LEFT_323_TM=59.348
PRIMER_RIGHT_323_TM=62.880
PRIMER_LEFT_323_GC_PERCENT=55.556
PRIMER_RIGHT_323_GC_PERCENT=55.000
PRIMER_LEFT_323_SELF_ANY=3.00
PRIMER_RIGHT_323_SELF_ANY=2.00
PRIMER_LEFT_323_SELF_END=1.00
PRIMER_RIGHT_323_SELF_END=0.00
PRIMER_LEFT_323_END_STABILITY=8.2000
PRIMER_RIGHT_323_END_STABILITY=7.9000
PRIMER_PAIR_323_COMPL_ANY=3.00
PRIMER_PAIR_323_COMPL_END=0.00
PRIMER_PAIR_323_PRODUCT_SIZE=99
PRIMER_PAIR_323_PRODUCT_TM=73.3
PRIMER_PAIR_324_PENALTY=5.533388
PRIMER_LEFT_324_PENALTY=2.901722
PRIMER_RIGHT_324_PENALTY=2.631665
PRIMER_LEFT_324_SEQUENCE=AGTGCAAGACCCATCCCC
PRIMER_RIGHT_324_SEQUENCE=TCAGGAACGAGAAGGGCATC
PRIMER_LEFT_324=510,18
PRIMER_RIGHT_324=591,20
PRIMER_LEFT_324_TM=60.902
PRIMER_RIGHT_324_TM=62.632
PRIMER_LEFT_324_GC_PERCENT=61.111
PRIMER_RIGHT_324_GC_PERCENT=55.000
PRIMER_LEFT_324_SELF_ANY=4.00
PRIMER_RIGHT_324_SELF_ANY=2.00
PRIMER_LEFT_324_SELF_END=0.00
PRIMER_RIGHT_324_SELF_END=2.00
PRIMER_LEFT_324_END_STABILITY=10.9000
PRIMER_RIGHT_324_END_STABILITY=8.1000
PRIMER_PAIR_324_COMPL_ANY=4.00
PRIMER_PAIR_324_COMPL_END=3.00
PRIMER_PAIR_324_PRODUCT_SIZE=82
PRIMER_PAIR_324_PRODUCT_TM=73.1
PRIMER_PAIR_325_PENALTY=5.541878
PRIMER_LEFT_325_PENALTY=3.081950
PRIMER_RIGHT_325_PENALTY=2.459929
PRIMER_LEFT_325_SEQUENCE=CAACCAGTGACCATCCAGA
PRIMER_RIGHT_325_SEQUENCE=CAACTAAGCAGCGGTAGGG
PRIMER_LEFT_325=467,19
PRIMER_RIGHT_325=557,19
PRIMER_LEFT_325_TM=57.918
PRIMER_RIGHT_325_TM=58.540
PRIMER_LEFT_325_GC_PERCENT=52.632
PRIMER_RIGHT_325_GC_PERCENT=57.895
PRIMER_LEFT_325_SELF_ANY=3.00
PRIMER_RIGHT_325_SELF_ANY=3.00
PRIMER_LEFT_325_SELF_END=2.00
PRIMER_RIGHT_325_SELF_END=0.00
PRIMER_LEFT_325_END_STABILITY=8.2000
PRIMER_RIGHT_325_END_STABILITY=8.7000
PRIMER_PAIR_325_COMPL_ANY=3.00
PRIMER_PAIR_325_COMPL_END=1.00
PRIMER_PAIR_325_PRODUCT_SIZE=91
PRIMER_PAIR_325_PRODUCT_TM=76.7
PRIMER_PAIR_326_PENALTY=5.548354
PRIMER_LEFT_326_PENALTY=2.605621
PRIMER_RIGHT_326_PENALTY=2.942733
PRIMER_LEFT_326_SEQUENCE=CGTTCCTGACAAGTGCAAA
PRIMER_RIGHT_326_SEQUENCE=CTGCATGTCTCTTTGGCG
PRIMER_LEFT_326=583,19
PRIMER_RIGHT_326=675,18
PRIMER_LEFT_326_TM=58.394
PRIMER_RIGHT_326_TM=59.057
PRIMER_LEFT_326_GC_PERCENT=47.368
PRIMER_RIGHT_326_GC_PERCENT=55.556
PRIMER_LEFT_326_SELF_ANY=4.00
PRIMER_RIGHT_326_SELF_ANY=4.00
PRIMER_LEFT_326_SELF_END=0.00
PRIMER_RIGHT_326_SELF_END=2.00
PRIMER_LEFT_326_END_STABILITY=8.8000
PRIMER_RIGHT_326_END_STABILITY=11.7000
PRIMER_PAIR_326_COMPL_ANY=4.00
PRIMER_PAIR_326_COMPL_END=2.00
PRIMER_PAIR_326_PRODUCT_SIZE=93
PRIMER_PAIR_326_PRODUCT_TM=73.7
PRIMER_PAIR_327_PENALTY=5.554846
PRIMER_LEFT_327_PENALTY=2.600038
PRIMER_RIGHT_327_PENALTY=2.954809
PRIMER_LEFT_327_SEQUENCE=TCCCCACTTTGTGATTCCCTA
PRIMER_RIGHT_327_SEQUENCE=TGCACTTGTCAGGAACGAGAA
PRIMER_LEFT_327=523,21
PRIMER_RIGHT_327=599,21
PRIMER_LEFT_327_TM=61.600
PRIMER_RIGHT_327_TM=61.955
PRIMER_LEFT_327_GC_PERCENT=47.619
PRIMER_RIGHT_327_GC_PERCENT=47.619
PRIMER_LEFT_327_SELF_ANY=3.00
PRIMER_RIGHT_327_SELF_ANY=4.00
PRIMER_LEFT_327_SELF_END=3.00
PRIMER_RIGHT_327_SELF_END=1.00
PRIMER_LEFT_327_END_STABILITY=8.7000
PRIMER_RIGHT_327_END_STABILITY=6.7000
PRIMER_PAIR_327_COMPL_ANY=4.00
PRIMER_PAIR_327_COMPL_END=3.00
PRIMER_PAIR_327_PRODUCT_SIZE=77
PRIMER_PAIR_327_PRODUCT_TM=72.3
PRIMER_PAIR_328_PENALTY=5.569766
PRIMER_LEFT_328_PENALTY=1.651669
PRIMER_RIGHT_328_PENALTY=3.918097
PRIMER_LEFT_328_SEQUENCE=TCCATCAGGGACCAAAACCT
PRIMER_RIGHT_328_SEQUENCE=CTTCTACCACATTGGTGATCTGC
PRIMER_LEFT_328=364,20
PRIMER_RIGHT_328=461,23
PRIMER_LEFT_328_TM=61.652
PRIMER_RIGHT_328_TM=60.918
PRIMER_LEFT_328_GC_PERCENT=50.000
PRIMER_RIGHT_328_GC_PERCENT=47.826
PRIMER_LEFT_328_SELF_ANY=5.00
PRIMER_RIGHT_328_SELF_ANY=6.00
PRIMER_LEFT_328_SELF_END=0.00
PRIMER_RIGHT_328_SELF_END=2.00
PRIMER_LEFT_328_END_STABILITY=7.9000
PRIMER_RIGHT_328_END_STABILITY=8.2000
PRIMER_PAIR_328_COMPL_ANY=5.00
PRIMER_PAIR_328_COMPL_END=0.00
PRIMER_PAIR_328_PRODUCT_SIZE=98
PRIMER_PAIR_328_PRODUCT_TM=73.4
PRIMER_PAIR_329_PENALTY=5.604774
PRIMER_LEFT_329_PENALTY=3.368077
PRIMER_RIGHT_329_PENALTY=2.236697
PRIMER_LEFT_329_SEQUENCE=ACATGCACATGAATGTCCAGAA
PRIMER_RIGHT_329_SEQUENCE=CCTTGGTATCAATGCAGGTTTT
PRIMER_LEFT_329=324,22
PRIMER_RIGHT_329=398,22
PRIMER_LEFT_329_TM=61.368
PRIMER_RIGHT_329_TM=60.237
PRIMER_LEFT_329_GC_PERCENT=40.909
PRIMER_RIGHT_329_GC_PERCENT=40.909
PRIMER_LEFT_329_SELF_ANY=8.00
PRIMER_RIGHT_329_SELF_ANY=6.00
PRIMER_LEFT_329_SELF_END=3.00
PRIMER_RIGHT_329_SELF_END=0.00
PRIMER_LEFT_329_END_STABILITY=7.0000
PRIMER_RIGHT_329_END_STABILITY=7.0000
PRIMER_PAIR_329_COMPL_ANY=5.00
PRIMER_PAIR_329_COMPL_END=2.00
PRIMER_PAIR_329_PRODUCT_SIZE=75
PRIMER_PAIR_329_PRODUCT_TM=71.0
PRIMER_PAIR_330_PENALTY=5.621018
PRIMER_LEFT_330_PENALTY=3.161089
PRIMER_RIGHT_330_PENALTY=2.459929
PRIMER_LEFT_330_SEQUENCE=CCAACCAACCAGTGACCA
PRIMER_RIGHT_330_SEQUENCE=CCAACTAAGCAGCGGTAGG
PRIMER_LEFT_330=462,18
PRIMER_RIGHT_330=558,19
PRIMER_LEFT_330_TM=58.839
PRIMER_RIGHT_330_TM=58.540
PRIMER_LEFT_330_GC_PERCENT=55.556
PRIMER_RIGHT_330_GC_PERCENT=57.895
PRIMER_LEFT_330_SELF_ANY=3.00
PRIMER_RIGHT_330_SELF_ANY=3.00
PRIMER_LEFT_330_SELF_END=2.00
PRIMER_RIGHT_330_SELF_END=1.00
PRIMER_LEFT_330_END_STABILITY=7.9000
PRIMER_RIGHT_330_END_STABILITY=6.9000
PRIMER_PAIR_330_COMPL_ANY=4.00
PRIMER_PAIR_330_COMPL_END=2.00
PRIMER_PAIR_330_PRODUCT_SIZE=97
PRIMER_PAIR_330_PRODUCT_TM=77.4
PRIMER_PAIR_331_PENALTY=5.624235
PRIMER_LEFT_331_PENALTY=2.294916
PRIMER_RIGHT_331_PENALTY=3.329319
PRIMER_LEFT_331_SEQUENCE=CACCAGGAGAGGATGGATGTT
PRIMER_RIGHT_331_SEQUENCE=TCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_331=608,21
PRIMER_RIGHT_331=683,23
PRIMER_LEFT_331_TM=61.295
PRIMER_RIGHT_331_TM=59.671
PRIMER_LEFT_331_GC_PERCENT=52.381
PRIMER_RIGHT_331_GC_PERCENT=43.478
PRIMER_LEFT_331_SELF_ANY=3.00
PRIMER_RIGHT_331_SELF_ANY=4.00
PRIMER_LEFT_331_SELF_END=0.00
PRIMER_RIGHT_331_SELF_END=0.00
PRIMER_LEFT_331_END_STABILITY=6.6000
PRIMER_RIGHT_331_END_STABILITY=7.3000
PRIMER_PAIR_331_COMPL_ANY=6.00
PRIMER_PAIR_331_COMPL_END=3.00
PRIMER_PAIR_331_PRODUCT_SIZE=76
PRIMER_PAIR_331_PRODUCT_TM=73.0
PRIMER_PAIR_332_PENALTY=5.627148
PRIMER_LEFT_332_PENALTY=1.709051
PRIMER_RIGHT_332_PENALTY=3.918097
PRIMER_LEFT_332_SEQUENCE=TCCATCAGGGACCAAAACC
PRIMER_RIGHT_332_SEQUENCE=GCTTCTACCACATTGGTGATCTG
PRIMER_LEFT_332=364,19
PRIMER_RIGHT_332=462,23
PRIMER_LEFT_332_TM=60.709
PRIMER_RIGHT_332_TM=60.918
PRIMER_LEFT_332_GC_PERCENT=52.632
PRIMER_RIGHT_332_GC_PERCENT=47.826
PRIMER_LEFT_332_SELF_ANY=5.00
PRIMER_RIGHT_332_SELF_ANY=6.00
PRIMER_LEFT_332_SELF_END=0.00
PRIMER_RIGHT_332_SELF_END=1.00
PRIMER_LEFT_332_END_STABILITY=8.2000
PRIMER_RIGHT_332_END_STABILITY=6.6000
PRIMER_PAIR_332_COMPL_ANY=5.00
PRIMER_PAIR_332_COMPL_END=1.00
PRIMER_PAIR_332_PRODUCT_SIZE=99
PRIMER_PAIR_332_PRODUCT_TM=73.7
PRIMER_PAIR_333_PENALTY=5.636669
PRIMER_LEFT_333_PENALTY=1.309943
PRIMER_RIGHT_333_PENALTY=4.326727
PRIMER_LEFT_333_SEQUENCE=GTCCAGAATGGGAAGTGGG
PRIMER_RIGHT_333_SEQUENCE=TTCAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_333=338,19
PRIMER_RIGHT_333=436,23
PRIMER_LEFT_333_TM=60.310
PRIMER_RIGHT_333_TM=61.327
PRIMER_LEFT_333_GC_PERCENT=57.895
PRIMER_RIGHT_333_GC_PERCENT=43.478
PRIMER_LEFT_333_SELF_ANY=3.00
PRIMER_RIGHT_333_SELF_ANY=5.00
PRIMER_LEFT_333_SELF_END=0.00
PRIMER_RIGHT_333_SELF_END=3.00
PRIMER_LEFT_333_END_STABILITY=9.4000
PRIMER_RIGHT_333_END_STABILITY=8.4000
PRIMER_PAIR_333_COMPL_ANY=5.00
PRIMER_PAIR_333_COMPL_END=1.00
PRIMER_PAIR_333_PRODUCT_SIZE=99
PRIMER_PAIR_333_PRODUCT_TM=73.7
PRIMER_PAIR_334_PENALTY=5.650718
PRIMER_LEFT_334_PENALTY=3.163121
PRIMER_RIGHT_334_PENALTY=2.487598
PRIMER_LEFT_334_SEQUENCE=AACCAACCAGTGACCATCCAG
PRIMER_RIGHT_334_SEQUENCE=TAAGCAGCGGTAGGGAATCAC
PRIMER_LEFT_334=464,21
PRIMER_RIGHT_334=553,21
PRIMER_LEFT_334_TM=62.163
PRIMER_RIGHT_334_TM=61.488
PRIMER_LEFT_334_GC_PERCENT=52.381
PRIMER_RIGHT_334_GC_PERCENT=52.381
PRIMER_LEFT_334_SELF_ANY=3.00
PRIMER_RIGHT_334_SELF_ANY=3.00
PRIMER_LEFT_334_SELF_END=1.00
PRIMER_RIGHT_334_SELF_END=3.00
PRIMER_LEFT_334_END_STABILITY=8.2000
PRIMER_RIGHT_334_END_STABILITY=6.3000
PRIMER_PAIR_334_COMPL_ANY=4.00
PRIMER_PAIR_334_COMPL_END=1.00
PRIMER_PAIR_334_PRODUCT_SIZE=90
PRIMER_PAIR_334_PRODUCT_TM=76.5
PRIMER_PAIR_335_PENALTY=5.655515
PRIMER_LEFT_335_PENALTY=2.782312
PRIMER_RIGHT_335_PENALTY=2.873203
PRIMER_LEFT_335_SEQUENCE=GGTGAGTTTGTAAGTGATGCCC
PRIMER_RIGHT_335_SEQUENCE=TTTCGCAAACATCCATCCTCT
PRIMER_LEFT_335=557,22
PRIMER_RIGHT_335=635,21
PRIMER_LEFT_335_TM=60.782
PRIMER_RIGHT_335_TM=61.873
PRIMER_LEFT_335_GC_PERCENT=50.000
PRIMER_RIGHT_335_GC_PERCENT=42.857
PRIMER_LEFT_335_SELF_ANY=2.00
PRIMER_RIGHT_335_SELF_ANY=4.00
PRIMER_LEFT_335_SELF_END=0.00
PRIMER_RIGHT_335_SELF_END=0.00
PRIMER_LEFT_335_END_STABILITY=11.2000
PRIMER_RIGHT_335_END_STABILITY=7.9000
PRIMER_PAIR_335_COMPL_ANY=5.00
PRIMER_PAIR_335_COMPL_END=1.00
PRIMER_PAIR_335_PRODUCT_SIZE=79
PRIMER_PAIR_335_PRODUCT_TM=71.0
PRIMER_PAIR_336_PENALTY=5.664193
PRIMER_LEFT_336_PENALTY=2.247631
PRIMER_RIGHT_336_PENALTY=3.416562
PRIMER_LEFT_336_SEQUENCE=ATGTTTGCGAAACTCATCTTCA
PRIMER_RIGHT_336_SEQUENCE=GGCAGCAACATGCCGTAG
PRIMER_LEFT_336=624,22
PRIMER_RIGHT_336=717,18
PRIMER_LEFT_336_TM=59.752
PRIMER_RIGHT_336_TM=61.417
PRIMER_LEFT_336_GC_PERCENT=36.364
PRIMER_RIGHT_336_GC_PERCENT=61.111
PRIMER_LEFT_336_SELF_ANY=6.00
PRIMER_RIGHT_336_SELF_ANY=4.00
PRIMER_LEFT_336_SELF_END=1.00
PRIMER_RIGHT_336_SELF_END=0.00
PRIMER_LEFT_336_END_STABILITY=7.0000
PRIMER_RIGHT_336_END_STABILITY=7.4000
PRIMER_PAIR_336_COMPL_ANY=5.00
PRIMER_PAIR_336_COMPL_END=1.00
PRIMER_PAIR_336_PRODUCT_SIZE=94
PRIMER_PAIR_336_PRODUCT_TM=74.0
PRIMER_PAIR_337_PENALTY=5.666192
PRIMER_LEFT_337_PENALTY=3.514925
PRIMER_RIGHT_337_PENALTY=2.151267
PRIMER_LEFT_337_SEQUENCE=GCTGGCTGAACCCCAGATT
PRIMER_RIGHT_337_SEQUENCE=TGATGGATCTGAATCCCACT
PRIMER_LEFT_337=283,19
PRIMER_RIGHT_337=370,20
PRIMER_LEFT_337_TM=62.515
PRIMER_RIGHT_337_TM=57.849
PRIMER_LEFT_337_GC_PERCENT=57.895
PRIMER_RIGHT_337_GC_PERCENT=45.000
PRIMER_LEFT_337_SELF_ANY=4.00
PRIMER_RIGHT_337_SELF_ANY=5.00
PRIMER_LEFT_337_SELF_END=1.00
PRIMER_RIGHT_337_SELF_END=1.00
PRIMER_LEFT_337_END_STABILITY=6.6000
PRIMER_RIGHT_337_END_STABILITY=7.9000
PRIMER_PAIR_337_COMPL_ANY=5.00
PRIMER_PAIR_337_COMPL_END=1.00
PRIMER_PAIR_337_PRODUCT_SIZE=88
PRIMER_PAIR_337_PRODUCT_TM=73.6
PRIMER_PAIR_338_PENALTY=5.685447
PRIMER_LEFT_338_PENALTY=3.600004
PRIMER_RIGHT_338_PENALTY=2.085443
PRIMER_LEFT_338_SEQUENCE=GCTGAACCCCAGATTGCC
PRIMER_RIGHT_338_SEQUENCE=CAGGTTTTGGTCCCTGATG
PRIMER_LEFT_338=287,18
PRIMER_RIGHT_338=384,19
PRIMER_LEFT_338_TM=61.600
PRIMER_RIGHT_338_TM=58.915
PRIMER_LEFT_338_GC_PERCENT=61.111
PRIMER_RIGHT_338_GC_PERCENT=52.632
PRIMER_LEFT_338_SELF_ANY=3.00
PRIMER_RIGHT_338_SELF_ANY=4.00
PRIMER_LEFT_338_SELF_END=1.00
PRIMER_RIGHT_338_SELF_END=1.00
PRIMER_LEFT_338_END_STABILITY=10.0000
PRIMER_RIGHT_338_END_STABILITY=6.9000
PRIMER_PAIR_338_COMPL_ANY=4.00
PRIMER_PAIR_338_COMPL_END=1.00
PRIMER_PAIR_338_PRODUCT_SIZE=98
PRIMER_PAIR_338_PRODUCT_TM=74.3
PRIMER_PAIR_339_PENALTY=5.739879
PRIMER_LEFT_339_PENALTY=2.803130
PRIMER_RIGHT_339_PENALTY=2.936750
PRIMER_LEFT_339_SEQUENCE=GTGATTCCCTACCGCTGC
PRIMER_RIGHT_339_SEQUENCE=GCAAACATCCATCCTCTCCTG
PRIMER_LEFT_339=533,18
PRIMER_RIGHT_339=631,21
PRIMER_LEFT_339_TM=59.197
PRIMER_RIGHT_339_TM=61.937
PRIMER_LEFT_339_GC_PERCENT=61.111
PRIMER_RIGHT_339_GC_PERCENT=52.381
PRIMER_LEFT_339_SELF_ANY=3.00
PRIMER_RIGHT_339_SELF_ANY=2.00
PRIMER_LEFT_339_SELF_END=3.00
PRIMER_RIGHT_339_SELF_END=1.00
PRIMER_LEFT_339_END_STABILITY=9.7000
PRIMER_RIGHT_339_END_STABILITY=8.2000
PRIMER_PAIR_339_COMPL_ANY=3.00
PRIMER_PAIR_339_COMPL_END=1.00
PRIMER_PAIR_339_PRODUCT_SIZE=99
PRIMER_PAIR_339_PRODUCT_TM=73.3
PRIMER_PAIR_340_PENALTY=5.751036
PRIMER_LEFT_340_PENALTY=3.525975
PRIMER_RIGHT_340_PENALTY=2.225062
PRIMER_LEFT_340_SEQUENCE=TGGAGGTACCCACTGATGGTAA
PRIMER_RIGHT_340_SEQUENCE=TTCCCATTCTGGACATTCAT
PRIMER_LEFT_340=252,22
PRIMER_RIGHT_340=351,20
PRIMER_LEFT_340_TM=61.526
PRIMER_RIGHT_340_TM=57.775
PRIMER_LEFT_340_GC_PERCENT=50.000
PRIMER_RIGHT_340_GC_PERCENT=40.000
PRIMER_LEFT_340_SELF_ANY=6.00
PRIMER_RIGHT_340_SELF_ANY=3.00
PRIMER_LEFT_340_SELF_END=3.00
PRIMER_RIGHT_340_SELF_END=2.00
PRIMER_LEFT_340_END_STABILITY=7.2000
PRIMER_RIGHT_340_END_STABILITY=6.9000
PRIMER_PAIR_340_COMPL_ANY=5.00
PRIMER_PAIR_340_COMPL_END=3.00
PRIMER_PAIR_340_PRODUCT_SIZE=100
PRIMER_PAIR_340_PRODUCT_TM=75.2
PRIMER_PAIR_341_PENALTY=5.751270
PRIMER_LEFT_341_PENALTY=3.600004
PRIMER_RIGHT_341_PENALTY=2.151267
PRIMER_LEFT_341_SEQUENCE=GGCTGAACCCCAGATTGC
PRIMER_RIGHT_341_SEQUENCE=CTGATGGATCTGAATCCCAC
PRIMER_LEFT_341=286,18
PRIMER_RIGHT_341=371,20
PRIMER_LEFT_341_TM=61.600
PRIMER_RIGHT_341_TM=57.849
PRIMER_LEFT_341_GC_PERCENT=61.111
PRIMER_RIGHT_341_GC_PERCENT=50.000
PRIMER_LEFT_341_SELF_ANY=3.00
PRIMER_RIGHT_341_SELF_ANY=5.00
PRIMER_LEFT_341_SELF_END=2.00
PRIMER_RIGHT_341_SELF_END=3.00
PRIMER_LEFT_341_END_STABILITY=8.4000
PRIMER_RIGHT_341_END_STABILITY=9.4000
PRIMER_PAIR_341_COMPL_ANY=5.00
PRIMER_PAIR_341_COMPL_END=0.00
PRIMER_PAIR_341_PRODUCT_SIZE=86
PRIMER_PAIR_341_PRODUCT_TM=73.4
PRIMER_PAIR_342_PENALTY=5.753688
PRIMER_LEFT_342_PENALTY=2.646135
PRIMER_RIGHT_342_PENALTY=3.107553
PRIMER_LEFT_342_SEQUENCE=GCCCTTCTCGTTCCTGACAA
PRIMER_RIGHT_342_SEQUENCE=CATGTCTCTTTGGCGACG
PRIMER_LEFT_342=575,20
PRIMER_RIGHT_342=672,18
PRIMER_LEFT_342_TM=62.646
PRIMER_RIGHT_342_TM=58.892
PRIMER_LEFT_342_GC_PERCENT=55.000
PRIMER_RIGHT_342_GC_PERCENT=55.556
PRIMER_LEFT_342_SELF_ANY=3.00
PRIMER_RIGHT_342_SELF_ANY=4.00
PRIMER_LEFT_342_SELF_END=1.00
PRIMER_RIGHT_342_SELF_END=3.00
PRIMER_LEFT_342_END_STABILITY=6.7000
PRIMER_RIGHT_342_END_STABILITY=10.1000
PRIMER_PAIR_342_COMPL_ANY=4.00
PRIMER_PAIR_342_COMPL_END=2.00
PRIMER_PAIR_342_PRODUCT_SIZE=98
PRIMER_PAIR_342_PRODUCT_TM=74.3
PRIMER_PAIR_343_PENALTY=5.767664
PRIMER_LEFT_343_PENALTY=3.220337
PRIMER_RIGHT_343_PENALTY=2.547327
PRIMER_LEFT_343_SEQUENCE=TGTGGTAGAAGCCAACCAACC
PRIMER_RIGHT_343_SEQUENCE=GGATGGGTCTTGCACTGCTT
PRIMER_LEFT_343=451,21
PRIMER_RIGHT_343=525,20
PRIMER_LEFT_343_TM=62.220
PRIMER_RIGHT_343_TM=62.547
PRIMER_LEFT_343_GC_PERCENT=52.381
PRIMER_RIGHT_343_GC_PERCENT=55.000
PRIMER_LEFT_343_SELF_ANY=4.00
PRIMER_RIGHT_343_SELF_ANY=5.00
PRIMER_LEFT_343_SELF_END=1.00
PRIMER_RIGHT_343_SELF_END=1.00
PRIMER_LEFT_343_END_STABILITY=8.2000
PRIMER_RIGHT_343_END_STABILITY=8.5000
PRIMER_PAIR_343_COMPL_ANY=5.00
PRIMER_PAIR_343_COMPL_END=2.00
PRIMER_PAIR_343_PRODUCT_SIZE=75
PRIMER_PAIR_343_PRODUCT_TM=76.0
PRIMER_PAIR_344_PENALTY=5.767970
PRIMER_LEFT_344_PENALTY=3.540705
PRIMER_RIGHT_344_PENALTY=2.227266
PRIMER_LEFT_344_SEQUENCE=CCCACTGATGGTAATGCTG
PRIMER_RIGHT_344_SEQUENCE=TCCCACTTCCCATTCTGGAC
PRIMER_LEFT_344=260,19
PRIMER_RIGHT_344=357,20
PRIMER_LEFT_344_TM=57.459
PRIMER_RIGHT_344_TM=62.227
PRIMER_LEFT_344_GC_PERCENT=52.632
PRIMER_RIGHT_344_GC_PERCENT=55.000
PRIMER_LEFT_344_SELF_ANY=3.00
PRIMER_RIGHT_344_SELF_ANY=3.00
PRIMER_LEFT_344_SELF_END=1.00
PRIMER_RIGHT_344_SELF_END=1.00
PRIMER_LEFT_344_END_STABILITY=8.5000
PRIMER_RIGHT_344_END_STABILITY=7.9000
PRIMER_PAIR_344_COMPL_ANY=5.00
PRIMER_PAIR_344_COMPL_END=2.00
PRIMER_PAIR_344_PRODUCT_SIZE=98
PRIMER_PAIR_344_PRODUCT_TM=75.5
PRIMER_PAIR_345_PENALTY=5.786788
PRIMER_LEFT_345_PENALTY=2.831979
PRIMER_RIGHT_345_PENALTY=2.954809
PRIMER_LEFT_345_SEQUENCE=ATCCCCACTTTGTGATTCCCT
PRIMER_RIGHT_345_SEQUENCE=TTGCACTTGTCAGGAACGAGA
PRIMER_LEFT_345=522,21
PRIMER_RIGHT_345=600,21
PRIMER_LEFT_345_TM=61.832
PRIMER_RIGHT_345_TM=61.955
PRIMER_LEFT_345_GC_PERCENT=47.619
PRIMER_RIGHT_345_GC_PERCENT=47.619
PRIMER_LEFT_345_SELF_ANY=4.00
PRIMER_RIGHT_345_SELF_ANY=4.00
PRIMER_LEFT_345_SELF_END=1.00
PRIMER_RIGHT_345_SELF_END=0.00
PRIMER_LEFT_345_END_STABILITY=9.4000
PRIMER_RIGHT_345_END_STABILITY=8.4000
PRIMER_PAIR_345_COMPL_ANY=4.00
PRIMER_PAIR_345_COMPL_END=2.00
PRIMER_PAIR_345_PRODUCT_SIZE=79
PRIMER_PAIR_345_PRODUCT_TM=72.0
PRIMER_PAIR_346_PENALTY=5.790016
PRIMER_LEFT_346_PENALTY=2.782339
PRIMER_RIGHT_346_PENALTY=3.007677
PRIMER_LEFT_346_SEQUENCE=CACTTTGTGATTCCCTACCGC
PRIMER_RIGHT_346_SEQUENCE=TCCATCCTCTCCTGGTGTAAGA
PRIMER_LEFT_346=527,21
PRIMER_RIGHT_346=624,22
PRIMER_LEFT_346_TM=61.782
PRIMER_RIGHT_346_TM=61.008
PRIMER_LEFT_346_GC_PERCENT=52.381
PRIMER_RIGHT_346_GC_PERCENT=50.000
PRIMER_LEFT_346_SELF_ANY=3.00
PRIMER_RIGHT_346_SELF_ANY=3.00
PRIMER_LEFT_346_SELF_END=2.00
PRIMER_RIGHT_346_SELF_END=3.00
PRIMER_LEFT_346_END_STABILITY=11.1000
PRIMER_RIGHT_346_END_STABILITY=6.0000
PRIMER_PAIR_346_COMPL_ANY=3.00
PRIMER_PAIR_346_COMPL_END=1.00
PRIMER_PAIR_346_PRODUCT_SIZE=98
PRIMER_PAIR_346_PRODUCT_TM=73.0
PRIMER_PAIR_347_PENALTY=5.793824
PRIMER_LEFT_347_PENALTY=2.577616
PRIMER_RIGHT_347_PENALTY=3.216208
PRIMER_LEFT_347_SEQUENCE=TAAGTGATGCCCTTCTCGTT
PRIMER_RIGHT_347_SEQUENCE=GTGTGCCAGTGAAGATGAGTT
PRIMER_LEFT_347=567,20
PRIMER_RIGHT_347=654,21
PRIMER_LEFT_347_TM=57.422
PRIMER_RIGHT_347_TM=57.784
PRIMER_LEFT_347_GC_PERCENT=45.000
PRIMER_RIGHT_347_GC_PERCENT=47.619
PRIMER_LEFT_347_SELF_ANY=4.00
PRIMER_RIGHT_347_SELF_ANY=3.00
PRIMER_LEFT_347_SELF_END=2.00
PRIMER_RIGHT_347_SELF_END=0.00
PRIMER_LEFT_347_END_STABILITY=8.4000
PRIMER_RIGHT_347_END_STABILITY=6.4000
PRIMER_PAIR_347_COMPL_ANY=4.00
PRIMER_PAIR_347_COMPL_END=0.00
PRIMER_PAIR_347_PRODUCT_SIZE=88
PRIMER_PAIR_347_PRODUCT_TM=72.2
PRIMER_PAIR_348_PENALTY=5.818629
PRIMER_LEFT_348_PENALTY=3.218591
PRIMER_RIGHT_348_PENALTY=2.600038
PRIMER_LEFT_348_SEQUENCE=CCAACCAGTGACCATCCA
PRIMER_RIGHT_348_SEQUENCE=TAGGGAATCACAAAGTGGGGA
PRIMER_LEFT_348=466,18
PRIMER_RIGHT_348=543,21
PRIMER_LEFT_348_TM=58.781
PRIMER_RIGHT_348_TM=61.600
PRIMER_LEFT_348_GC_PERCENT=55.556
PRIMER_RIGHT_348_GC_PERCENT=47.619
PRIMER_LEFT_348_SELF_ANY=3.00
PRIMER_RIGHT_348_SELF_ANY=3.00
PRIMER_LEFT_348_SELF_END=0.00
PRIMER_RIGHT_348_SELF_END=2.00
PRIMER_LEFT_348_END_STABILITY=8.1000
PRIMER_RIGHT_348_END_STABILITY=10.9000
PRIMER_PAIR_348_COMPL_ANY=4.00
PRIMER_PAIR_348_COMPL_END=2.00
PRIMER_PAIR_348_PRODUCT_SIZE=78
PRIMER_PAIR_348_PRODUCT_TM=75.9
PRIMER_PAIR_349_PENALTY=5.819733
PRIMER_LEFT_349_PENALTY=3.255899
PRIMER_RIGHT_349_PENALTY=2.563833
PRIMER_LEFT_349_SEQUENCE=CGCTGGAGGTACCCACTGA
PRIMER_RIGHT_349_SEQUENCE=TGTGCATGTTCAGTCTGCCA
PRIMER_LEFT_349=249,19
PRIMER_RIGHT_349=332,20
PRIMER_LEFT_349_TM=62.256
PRIMER_RIGHT_349_TM=62.564
PRIMER_LEFT_349_GC_PERCENT=63.158
PRIMER_RIGHT_349_GC_PERCENT=50.000
PRIMER_LEFT_349_SELF_ANY=6.00
PRIMER_RIGHT_349_SELF_ANY=5.00
PRIMER_LEFT_349_SELF_END=2.00
PRIMER_RIGHT_349_SELF_END=3.00
PRIMER_LEFT_349_END_STABILITY=6.4000
PRIMER_RIGHT_349_END_STABILITY=10.0000
PRIMER_PAIR_349_COMPL_ANY=5.00
PRIMER_PAIR_349_COMPL_END=2.00
PRIMER_PAIR_349_PRODUCT_SIZE=84
PRIMER_PAIR_349_PRODUCT_TM=75.7
PRIMER_PAIR_350_PENALTY=5.821778
PRIMER_LEFT_350_PENALTY=2.601441
PRIMER_RIGHT_350_PENALTY=3.220337
PRIMER_LEFT_350_SEQUENCE=GGACCAAAACCTGCATTGATAC
PRIMER_RIGHT_350_SEQUENCE=GGTTGGTTGGCTTCTACCACA
PRIMER_LEFT_350=372,22
PRIMER_RIGHT_350=471,21
PRIMER_LEFT_350_TM=60.601
PRIMER_RIGHT_350_TM=62.220
PRIMER_LEFT_350_GC_PERCENT=45.455
PRIMER_RIGHT_350_GC_PERCENT=52.381
PRIMER_LEFT_350_SELF_ANY=4.00
PRIMER_RIGHT_350_SELF_ANY=4.00
PRIMER_LEFT_350_SELF_END=2.00
PRIMER_RIGHT_350_SELF_END=2.00
PRIMER_LEFT_350_END_STABILITY=5.3000
PRIMER_RIGHT_350_END_STABILITY=8.2000
PRIMER_PAIR_350_COMPL_ANY=5.00
PRIMER_PAIR_350_COMPL_END=1.00
PRIMER_PAIR_350_PRODUCT_SIZE=100
PRIMER_PAIR_350_PRODUCT_TM=74.0
PRIMER_PAIR_351_PENALTY=5.827122
PRIMER_LEFT_351_PENALTY=1.312895
PRIMER_RIGHT_351_PENALTY=4.514227
PRIMER_LEFT_351_SEQUENCE=AGAATGGGAAGTGGGATTCAG
PRIMER_RIGHT_351_SEQUENCE=AGTTCAGGGTAGACTTCTTGGCA
PRIMER_LEFT_351=342,21
PRIMER_RIGHT_351=438,23
PRIMER_LEFT_351_TM=60.313
PRIMER_RIGHT_351_TM=61.514
PRIMER_LEFT_351_GC_PERCENT=47.619
PRIMER_RIGHT_351_GC_PERCENT=47.826
PRIMER_LEFT_351_SELF_ANY=4.00
PRIMER_RIGHT_351_SELF_ANY=4.00
PRIMER_LEFT_351_SELF_END=1.00
PRIMER_RIGHT_351_SELF_END=3.00
PRIMER_LEFT_351_END_STABILITY=7.0000
PRIMER_RIGHT_351_END_STABILITY=10.0000
PRIMER_PAIR_351_COMPL_ANY=5.00
PRIMER_PAIR_351_COMPL_END=0.00
PRIMER_PAIR_351_PRODUCT_SIZE=97
PRIMER_PAIR_351_PRODUCT_TM=73.2
PRIMER_PAIR_352_PENALTY=5.832952
PRIMER_LEFT_352_PENALTY=3.220337
PRIMER_RIGHT_352_PENALTY=2.612614
PRIMER_LEFT_352_SEQUENCE=GTGGTAGAAGCCAACCAACCA
PRIMER_RIGHT_352_SEQUENCE=CAAAGTGGGGATGGGTCTTG
PRIMER_LEFT_352=452,21
PRIMER_RIGHT_352=533,20
PRIMER_LEFT_352_TM=62.220
PRIMER_RIGHT_352_TM=62.613
PRIMER_LEFT_352_GC_PERCENT=52.381
PRIMER_RIGHT_352_GC_PERCENT=55.000
PRIMER_LEFT_352_SELF_ANY=4.00
PRIMER_RIGHT_352_SELF_ANY=3.00
PRIMER_LEFT_352_SELF_END=1.00
PRIMER_RIGHT_352_SELF_END=0.00
PRIMER_LEFT_352_END_STABILITY=8.2000
PRIMER_RIGHT_352_END_STABILITY=7.0000
PRIMER_PAIR_352_COMPL_ANY=4.00
PRIMER_PAIR_352_COMPL_END=3.00
PRIMER_PAIR_352_PRODUCT_SIZE=82
PRIMER_PAIR_352_PRODUCT_TM=76.6
PRIMER_PAIR_353_PENALTY=5.844497
PRIMER_LEFT_353_PENALTY=3.540705
PRIMER_RIGHT_353_PENALTY=2.303792
PRIMER_LEFT_353_SEQUENCE=CCACTGATGGTAATGCTGG
PRIMER_RIGHT_353_SEQUENCE=ACTTCCCATTCTGGACATTCAT
PRIMER_LEFT_353=261,19
PRIMER_RIGHT_353=353,22
PRIMER_LEFT_353_TM=57.459
PRIMER_RIGHT_353_TM=59.696
PRIMER_LEFT_353_GC_PERCENT=52.632
PRIMER_RIGHT_353_GC_PERCENT=40.909
PRIMER_LEFT_353_SELF_ANY=3.00
PRIMER_RIGHT_353_SELF_ANY=3.00
PRIMER_LEFT_353_SELF_END=0.00
PRIMER_RIGHT_353_SELF_END=2.00
PRIMER_LEFT_353_END_STABILITY=9.7000
PRIMER_RIGHT_353_END_STABILITY=6.9000
PRIMER_PAIR_353_COMPL_ANY=5.00
PRIMER_PAIR_353_COMPL_END=1.00
PRIMER_PAIR_353_PRODUCT_SIZE=93
PRIMER_PAIR_353_PRODUCT_TM=74.6
PRIMER_PAIR_354_PENALTY=5.848511
PRIMER_LEFT_354_PENALTY=2.118737
PRIMER_RIGHT_354_PENALTY=3.729774
PRIMER_LEFT_354_SEQUENCE=AATGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_354_SEQUENCE=GCAGGATGCCTTCCTTGG
PRIMER_LEFT_354=335,21
PRIMER_RIGHT_354=410,18
PRIMER_LEFT_354_TM=61.119
PRIMER_RIGHT_354_TM=61.730
PRIMER_LEFT_354_GC_PERCENT=47.619
PRIMER_RIGHT_354_GC_PERCENT=61.111
PRIMER_LEFT_354_SELF_ANY=3.00
PRIMER_RIGHT_354_SELF_ANY=7.00
PRIMER_LEFT_354_SELF_END=0.00
PRIMER_RIGHT_354_SELF_END=3.00
PRIMER_LEFT_354_END_STABILITY=7.9000
PRIMER_RIGHT_354_END_STABILITY=8.5000
PRIMER_PAIR_354_COMPL_ANY=5.00
PRIMER_PAIR_354_COMPL_END=0.00
PRIMER_PAIR_354_PRODUCT_SIZE=76
PRIMER_PAIR_354_PRODUCT_TM=72.0
PRIMER_PAIR_355_PENALTY=5.866430
PRIMER_LEFT_355_PENALTY=2.631665
PRIMER_RIGHT_355_PENALTY=3.234765
PRIMER_LEFT_355_SEQUENCE=GATGCCCTTCTCGTTCCTGA
PRIMER_RIGHT_355_SEQUENCE=GCCAGTGAAGATGAGTTTCGC
PRIMER_LEFT_355=572,20
PRIMER_RIGHT_355=650,21
PRIMER_LEFT_355_TM=62.632
PRIMER_RIGHT_355_TM=62.235
PRIMER_LEFT_355_GC_PERCENT=55.000
PRIMER_RIGHT_355_GC_PERCENT=52.381
PRIMER_LEFT_355_SELF_ANY=2.00
PRIMER_RIGHT_355_SELF_ANY=3.00
PRIMER_LEFT_355_SELF_END=2.00
PRIMER_RIGHT_355_SELF_END=3.00
PRIMER_LEFT_355_END_STABILITY=8.2000
PRIMER_RIGHT_355_END_STABILITY=10.2000
PRIMER_PAIR_355_COMPL_ANY=4.00
PRIMER_PAIR_355_COMPL_END=0.00
PRIMER_PAIR_355_PRODUCT_SIZE=79
PRIMER_PAIR_355_PRODUCT_TM=72.0
PRIMER_PAIR_356_PENALTY=5.889238
PRIMER_LEFT_356_PENALTY=2.840827
PRIMER_RIGHT_356_PENALTY=3.048411
PRIMER_LEFT_356_SEQUENCE=TTTGTGATTCCCTACCGCT
PRIMER_RIGHT_356_SEQUENCE=TCCTGGTGTAAGAATTTGCACTT
PRIMER_LEFT_356=530,19
PRIMER_RIGHT_356=615,23
PRIMER_LEFT_356_TM=58.159
PRIMER_RIGHT_356_TM=60.048
PRIMER_LEFT_356_GC_PERCENT=47.368
PRIMER_RIGHT_356_GC_PERCENT=39.130
PRIMER_LEFT_356_SELF_ANY=3.00
PRIMER_RIGHT_356_SELF_ANY=5.00
PRIMER_LEFT_356_SELF_END=2.00
PRIMER_RIGHT_356_SELF_END=2.00
PRIMER_LEFT_356_END_STABILITY=11.4000
PRIMER_RIGHT_356_END_STABILITY=6.7000
PRIMER_PAIR_356_COMPL_ANY=4.00
PRIMER_PAIR_356_COMPL_END=0.00
PRIMER_PAIR_356_PRODUCT_SIZE=86
PRIMER_PAIR_356_PRODUCT_TM=71.5
PRIMER_PAIR_357_PENALTY=5.902417
PRIMER_LEFT_357_PENALTY=2.954809
PRIMER_RIGHT_357_PENALTY=2.947608
PRIMER_LEFT_357_SEQUENCE=TCTCGTTCCTGACAAGTGCAA
PRIMER_RIGHT_357_SEQUENCE=CACTGCATGTCTCTTTGGCG
PRIMER_LEFT_357=580,21
PRIMER_RIGHT_357=677,20
PRIMER_LEFT_357_TM=61.955
PRIMER_RIGHT_357_TM=62.948
PRIMER_LEFT_357_GC_PERCENT=47.619
PRIMER_RIGHT_357_GC_PERCENT=55.000
PRIMER_LEFT_357_SELF_ANY=4.00
PRIMER_RIGHT_357_SELF_ANY=4.00
PRIMER_LEFT_357_SELF_END=2.00
PRIMER_RIGHT_357_SELF_END=2.00
PRIMER_LEFT_357_END_STABILITY=8.8000
PRIMER_RIGHT_357_END_STABILITY=11.7000
PRIMER_PAIR_357_COMPL_ANY=4.00
PRIMER_PAIR_357_COMPL_END=1.00
PRIMER_PAIR_357_PRODUCT_SIZE=98
PRIMER_PAIR_357_PRODUCT_TM=73.9
PRIMER_PAIR_358_PENALTY=5.907912
PRIMER_LEFT_358_PENALTY=2.668208
PRIMER_RIGHT_358_PENALTY=3.239704
PRIMER_LEFT_358_SEQUENCE=GTAAGTGATGCCCTTCTCGTT
PRIMER_RIGHT_358_SEQUENCE=ATGAGTTTCGCAAACATCCATC
PRIMER_LEFT_358=566,21
PRIMER_RIGHT_358=640,22
PRIMER_LEFT_358_TM=58.332
PRIMER_RIGHT_358_TM=61.240
PRIMER_LEFT_358_GC_PERCENT=47.619
PRIMER_RIGHT_358_GC_PERCENT=40.909
PRIMER_LEFT_358_SELF_ANY=4.00
PRIMER_RIGHT_358_SELF_ANY=6.00
PRIMER_LEFT_358_SELF_END=2.00
PRIMER_RIGHT_358_SELF_END=2.00
PRIMER_LEFT_358_END_STABILITY=8.4000
PRIMER_RIGHT_358_END_STABILITY=8.1000
PRIMER_PAIR_358_COMPL_ANY=4.00
PRIMER_PAIR_358_COMPL_END=1.00
PRIMER_PAIR_358_PRODUCT_SIZE=75
PRIMER_PAIR_358_PRODUCT_TM=70.5
PRIMER_PAIR_359_PENALTY=5.909864
PRIMER_LEFT_359_PENALTY=2.291669
PRIMER_RIGHT_359_PENALTY=3.618195
PRIMER_LEFT_359_SEQUENCE=TGTTTGCGAAACTCATCTTCAC
PRIMER_RIGHT_359_SEQUENCE=GCAGCAACATGCCGTAGT
PRIMER_LEFT_359=625,22
PRIMER_RIGHT_359=716,18
PRIMER_LEFT_359_TM=60.292
PRIMER_RIGHT_359_TM=58.382
PRIMER_LEFT_359_GC_PERCENT=40.909
PRIMER_RIGHT_359_GC_PERCENT=55.556
PRIMER_LEFT_359_SELF_ANY=6.00
PRIMER_RIGHT_359_SELF_ANY=4.00
PRIMER_LEFT_359_SELF_END=3.00
PRIMER_RIGHT_359_SELF_END=1.00
PRIMER_LEFT_359_END_STABILITY=6.7000
PRIMER_RIGHT_359_END_STABILITY=5.1000
PRIMER_PAIR_359_COMPL_ANY=4.00
PRIMER_PAIR_359_COMPL_END=2.00
PRIMER_PAIR_359_PRODUCT_SIZE=92
PRIMER_PAIR_359_PRODUCT_TM=73.9
PRIMER_PAIR_360_PENALTY=5.916750
PRIMER_LEFT_360_PENALTY=4.539189
PRIMER_RIGHT_360_PENALTY=1.377561
PRIMER_LEFT_360_SEQUENCE=GAGACATGCAGTGAGAAGAGTACC
PRIMER_RIGHT_360_SEQUENCE=GTGGGCAACACACAAACTCT
PRIMER_LEFT_360=665,24
PRIMER_RIGHT_360=764,20
PRIMER_LEFT_360_TM=59.461
PRIMER_RIGHT_360_TM=58.622
PRIMER_LEFT_360_GC_PERCENT=50.000
PRIMER_RIGHT_360_GC_PERCENT=50.000
PRIMER_LEFT_360_SELF_ANY=4.00
PRIMER_RIGHT_360_SELF_ANY=3.00
PRIMER_LEFT_360_SELF_END=2.00
PRIMER_RIGHT_360_SELF_END=1.00
PRIMER_LEFT_360_END_STABILITY=6.6000
PRIMER_RIGHT_360_END_STABILITY=6.1000
PRIMER_PAIR_360_COMPL_ANY=5.00
PRIMER_PAIR_360_COMPL_END=2.00
PRIMER_PAIR_360_PRODUCT_SIZE=100
PRIMER_PAIR_360_PRODUCT_TM=75.2
PRIMER_PAIR_361_PENALTY=5.958637
PRIMER_LEFT_361_PENALTY=2.840827
PRIMER_RIGHT_361_PENALTY=3.117810
PRIMER_LEFT_361_SEQUENCE=CTTTGTGATTCCCTACCGC
PRIMER_RIGHT_361_SEQUENCE=AAGAATTTGCACTTGTCAGGAA
PRIMER_LEFT_361=529,19
PRIMER_RIGHT_361=606,22
PRIMER_LEFT_361_TM=58.159
PRIMER_RIGHT_361_TM=58.882
PRIMER_LEFT_361_GC_PERCENT=52.632
PRIMER_RIGHT_361_GC_PERCENT=36.364
PRIMER_LEFT_361_SELF_ANY=3.00
PRIMER_RIGHT_361_SELF_ANY=4.00
PRIMER_LEFT_361_SELF_END=2.00
PRIMER_RIGHT_361_SELF_END=0.00
PRIMER_LEFT_361_END_STABILITY=11.1000
PRIMER_RIGHT_361_END_STABILITY=8.2000
PRIMER_PAIR_361_COMPL_ANY=4.00
PRIMER_PAIR_361_COMPL_END=1.00
PRIMER_PAIR_361_PRODUCT_SIZE=78
PRIMER_PAIR_361_PRODUCT_TM=70.6
PRIMER_PAIR_362_PENALTY=5.959057
PRIMER_LEFT_362_PENALTY=2.954879
PRIMER_RIGHT_362_PENALTY=3.004178
PRIMER_LEFT_362_SEQUENCE=GATTCCCTACCGCTGCTTAGTT
PRIMER_RIGHT_362_SEQUENCE=GCAAACATCCATCCTCTCC
PRIMER_LEFT_362=535,22
PRIMER_RIGHT_362=631,19
PRIMER_LEFT_362_TM=60.955
PRIMER_RIGHT_362_TM=57.996
PRIMER_LEFT_362_GC_PERCENT=50.000
PRIMER_RIGHT_362_GC_PERCENT=52.632
PRIMER_LEFT_362_SELF_ANY=3.00
PRIMER_RIGHT_362_SELF_ANY=2.00
PRIMER_LEFT_362_SELF_END=0.00
PRIMER_RIGHT_362_SELF_END=0.00
PRIMER_LEFT_362_END_STABILITY=5.7000
PRIMER_RIGHT_362_END_STABILITY=7.9000
PRIMER_PAIR_362_COMPL_ANY=3.00
PRIMER_PAIR_362_COMPL_END=0.00
PRIMER_PAIR_362_PRODUCT_SIZE=97
PRIMER_PAIR_362_PRODUCT_TM=73.2
PRIMER_PAIR_363_PENALTY=5.964597
PRIMER_LEFT_363_PENALTY=3.363116
PRIMER_RIGHT_363_PENALTY=2.601481
PRIMER_LEFT_363_SEQUENCE=CAACCAGTGACCATCCAGAACT
PRIMER_RIGHT_363_SEQUENCE=CTAAGCAGCGGTAGGGAATCA
PRIMER_LEFT_363=467,22
PRIMER_RIGHT_363=554,21
PRIMER_LEFT_363_TM=61.363
PRIMER_RIGHT_363_TM=61.601
PRIMER_LEFT_363_GC_PERCENT=50.000
PRIMER_RIGHT_363_GC_PERCENT=52.381
PRIMER_LEFT_363_SELF_ANY=3.00
PRIMER_RIGHT_363_SELF_ANY=3.00
PRIMER_LEFT_363_SELF_END=2.00
PRIMER_RIGHT_363_SELF_END=1.00
PRIMER_LEFT_363_END_STABILITY=6.4000
PRIMER_RIGHT_363_END_STABILITY=6.9000
PRIMER_PAIR_363_COMPL_ANY=3.00
PRIMER_PAIR_363_COMPL_END=1.00
PRIMER_PAIR_363_PRODUCT_SIZE=88
PRIMER_PAIR_363_PRODUCT_TM=76.8
PRIMER_PAIR_364_PENALTY=5.973566
PRIMER_LEFT_364_PENALTY=3.333224
PRIMER_RIGHT_364_PENALTY=2.640342
PRIMER_LEFT_364_SEQUENCE=TGGTAGAAGCCAACCAACCAG
PRIMER_RIGHT_364_SEQUENCE=CGGTAGGGAATCACAAAGTGG
PRIMER_LEFT_364=453,21
PRIMER_RIGHT_364=546,21
PRIMER_LEFT_364_TM=62.333
PRIMER_RIGHT_364_TM=61.640
PRIMER_LEFT_364_GC_PERCENT=52.381
PRIMER_RIGHT_364_GC_PERCENT=52.381
PRIMER_LEFT_364_SELF_ANY=4.00
PRIMER_RIGHT_364_SELF_ANY=3.00
PRIMER_LEFT_364_SELF_END=1.00
PRIMER_RIGHT_364_SELF_END=1.00
PRIMER_LEFT_364_END_STABILITY=7.9000
PRIMER_RIGHT_364_END_STABILITY=7.9000
PRIMER_PAIR_364_COMPL_ANY=4.00
PRIMER_PAIR_364_COMPL_END=2.00
PRIMER_PAIR_364_PRODUCT_SIZE=94
PRIMER_PAIR_364_PRODUCT_TM=77.1
PRIMER_PAIR_365_PENALTY=6.025252
PRIMER_LEFT_365_PENALTY=4.240356
PRIMER_RIGHT_365_PENALTY=1.784896
PRIMER_LEFT_365_SEQUENCE=CAAAGAGACATGCAGTGAGAAG
PRIMER_RIGHT_365_SEQUENCE=CACACAAACTCTACCCCTCG
PRIMER_LEFT_365=661,22
PRIMER_RIGHT_365=756,20
PRIMER_LEFT_365_TM=57.760
PRIMER_RIGHT_365_TM=58.215
PRIMER_LEFT_365_GC_PERCENT=45.455
PRIMER_RIGHT_365_GC_PERCENT=55.000
PRIMER_LEFT_365_SELF_ANY=4.00
PRIMER_RIGHT_365_SELF_ANY=2.00
PRIMER_LEFT_365_SELF_END=0.00
PRIMER_RIGHT_365_SELF_END=2.00
PRIMER_LEFT_365_END_STABILITY=6.7000
PRIMER_RIGHT_365_END_STABILITY=9.9000
PRIMER_PAIR_365_COMPL_ANY=4.00
PRIMER_PAIR_365_COMPL_END=1.00
PRIMER_PAIR_365_PRODUCT_SIZE=96
PRIMER_PAIR_365_PRODUCT_TM=74.2
PRIMER_PAIR_366_PENALTY=6.044911
PRIMER_LEFT_366_PENALTY=1.590836
PRIMER_RIGHT_366_PENALTY=4.454075
PRIMER_LEFT_366_SEQUENCE=CAGATCCATCAGGGACCAA
PRIMER_RIGHT_366_SEQUENCE=GATCTGCAGTTCAGGGTAGACT
PRIMER_LEFT_366=360,19
PRIMER_RIGHT_366=445,22
PRIMER_LEFT_366_TM=59.409
PRIMER_RIGHT_366_TM=57.546
PRIMER_LEFT_366_GC_PERCENT=52.632
PRIMER_RIGHT_366_GC_PERCENT=50.000
PRIMER_LEFT_366_SELF_ANY=5.00
PRIMER_RIGHT_366_SELF_ANY=8.00
PRIMER_LEFT_366_SELF_END=1.00
PRIMER_RIGHT_366_SELF_END=3.00
PRIMER_LEFT_366_END_STABILITY=8.2000
PRIMER_RIGHT_366_END_STABILITY=6.1000
PRIMER_PAIR_366_COMPL_ANY=6.00
PRIMER_PAIR_366_COMPL_END=1.00
PRIMER_PAIR_366_PRODUCT_SIZE=86
PRIMER_PAIR_366_PRODUCT_TM=72.9
PRIMER_PAIR_367_PENALTY=6.053359
PRIMER_LEFT_367_PENALTY=3.430130
PRIMER_RIGHT_367_PENALTY=2.623229
PRIMER_LEFT_367_SEQUENCE=ACCATCCAGAACTGGTGCAAG
PRIMER_RIGHT_367_SEQUENCE=CAACTAAGCAGCGGTAGGGAA
PRIMER_LEFT_367=476,21
PRIMER_RIGHT_367=557,21
PRIMER_LEFT_367_TM=62.430
PRIMER_RIGHT_367_TM=61.623
PRIMER_LEFT_367_GC_PERCENT=52.381
PRIMER_RIGHT_367_GC_PERCENT=52.381
PRIMER_LEFT_367_SELF_ANY=6.00
PRIMER_RIGHT_367_SELF_ANY=3.00
PRIMER_LEFT_367_SELF_END=2.00
PRIMER_RIGHT_367_SELF_END=0.00
PRIMER_LEFT_367_END_STABILITY=8.5000
PRIMER_RIGHT_367_END_STABILITY=9.7000
PRIMER_PAIR_367_COMPL_ANY=4.00
PRIMER_PAIR_367_COMPL_END=1.00
PRIMER_PAIR_367_PRODUCT_SIZE=82
PRIMER_PAIR_367_PRODUCT_TM=76.1
PRIMER_PAIR_368_PENALTY=6.058555
PRIMER_LEFT_368_PENALTY=4.183295
PRIMER_RIGHT_368_PENALTY=1.875260
PRIMER_LEFT_368_SEQUENCE=GTCGCCAAAGAGACATGC
PRIMER_RIGHT_368_SEQUENCE=ACTCTACCCCTCGGAACTTGT
PRIMER_LEFT_368=656,18
PRIMER_RIGHT_368=749,21
PRIMER_LEFT_368_TM=57.817
PRIMER_RIGHT_368_TM=59.125
PRIMER_LEFT_368_GC_PERCENT=55.556
PRIMER_RIGHT_368_GC_PERCENT=52.381
PRIMER_LEFT_368_SELF_ANY=4.00
PRIMER_RIGHT_368_SELF_ANY=3.00
PRIMER_LEFT_368_SELF_END=2.00
PRIMER_RIGHT_368_SELF_END=2.00
PRIMER_LEFT_368_END_STABILITY=8.4000
PRIMER_RIGHT_368_END_STABILITY=6.7000
PRIMER_PAIR_368_COMPL_ANY=4.00
PRIMER_PAIR_368_COMPL_END=2.00
PRIMER_PAIR_368_PRODUCT_SIZE=94
PRIMER_PAIR_368_PRODUCT_TM=74.9
PRIMER_PAIR_369_PENALTY=6.070485
PRIMER_LEFT_369_PENALTY=3.883954
PRIMER_RIGHT_369_PENALTY=2.186531
PRIMER_LEFT_369_SEQUENCE=AACCCCAGATTGCCATGTTCT
PRIMER_RIGHT_369_SEQUENCE=AATGCAGGTTTTGGTCCCTG
PRIMER_LEFT_369=291,21
PRIMER_RIGHT_369=388,20
PRIMER_LEFT_369_TM=62.884
PRIMER_RIGHT_369_TM=62.187
PRIMER_LEFT_369_GC_PERCENT=47.619
PRIMER_RIGHT_369_GC_PERCENT=50.000
PRIMER_LEFT_369_SELF_ANY=4.00
PRIMER_RIGHT_369_SELF_ANY=4.00
PRIMER_LEFT_369_SELF_END=0.00
PRIMER_RIGHT_369_SELF_END=1.00
PRIMER_LEFT_369_END_STABILITY=6.4000
PRIMER_RIGHT_369_END_STABILITY=9.7000
PRIMER_PAIR_369_COMPL_ANY=4.00
PRIMER_PAIR_369_COMPL_END=0.00
PRIMER_PAIR_369_PRODUCT_SIZE=98
PRIMER_PAIR_369_PRODUCT_TM=73.4
PRIMER_PAIR_370_PENALTY=6.070629
PRIMER_LEFT_370_PENALTY=3.895812
PRIMER_RIGHT_370_PENALTY=2.174817
PRIMER_LEFT_370_SEQUENCE=CCATGTTCTGTGGCAGACT
PRIMER_RIGHT_370_SEQUENCE=GGTATCAATGCAGGTTTTGGT
PRIMER_LEFT_370=303,19
PRIMER_RIGHT_370=394,21
PRIMER_LEFT_370_TM=57.104
PRIMER_RIGHT_370_TM=58.825
PRIMER_LEFT_370_GC_PERCENT=52.632
PRIMER_RIGHT_370_GC_PERCENT=42.857
PRIMER_LEFT_370_SELF_ANY=5.00
PRIMER_RIGHT_370_SELF_ANY=4.00
PRIMER_LEFT_370_SELF_END=3.00
PRIMER_RIGHT_370_SELF_END=2.00
PRIMER_LEFT_370_END_STABILITY=6.1000
PRIMER_RIGHT_370_END_STABILITY=8.2000
PRIMER_PAIR_370_COMPL_ANY=3.00
PRIMER_PAIR_370_COMPL_END=1.00
PRIMER_PAIR_370_PRODUCT_SIZE=92
PRIMER_PAIR_370_PRODUCT_TM=73.0
PRIMER_PAIR_371_PENALTY=6.077946
PRIMER_LEFT_371_PENALTY=1.928663
PRIMER_RIGHT_371_PENALTY=4.149283
PRIMER_LEFT_371_SEQUENCE=TTACACCAGGAGAGGATGGA
PRIMER_RIGHT_371_SEQUENCE=CGTAGTCATGCAAGTTGGTACTC
PRIMER_LEFT_371=605,20
PRIMER_RIGHT_371=704,23
PRIMER_LEFT_371_TM=58.071
PRIMER_RIGHT_371_TM=58.851
PRIMER_LEFT_371_GC_PERCENT=50.000
PRIMER_RIGHT_371_GC_PERCENT=47.826
PRIMER_LEFT_371_SELF_ANY=3.00
PRIMER_RIGHT_371_SELF_ANY=5.00
PRIMER_LEFT_371_SELF_END=0.00
PRIMER_RIGHT_371_SELF_END=2.00
PRIMER_LEFT_371_END_STABILITY=8.1000
PRIMER_RIGHT_371_END_STABILITY=5.4000
PRIMER_PAIR_371_COMPL_ANY=4.00
PRIMER_PAIR_371_COMPL_END=2.00
PRIMER_PAIR_371_PRODUCT_SIZE=100
PRIMER_PAIR_371_PRODUCT_TM=74.0
PRIMER_PAIR_372_PENALTY=6.080957
PRIMER_LEFT_372_PENALTY=4.397153
PRIMER_RIGHT_372_PENALTY=1.683804
PRIMER_LEFT_372_SEQUENCE=TTGGCACTGCTCCTGCTG
PRIMER_RIGHT_372_SEQUENCE=AGAACATGGCAATCTGGGGT
PRIMER_LEFT_372=212,18
PRIMER_RIGHT_372=311,20
PRIMER_LEFT_372_TM=62.397
PRIMER_RIGHT_372_TM=61.684
PRIMER_LEFT_372_GC_PERCENT=61.111
PRIMER_RIGHT_372_GC_PERCENT=50.000
PRIMER_LEFT_372_SELF_ANY=5.00
PRIMER_RIGHT_372_SELF_ANY=4.00
PRIMER_LEFT_372_SELF_END=2.00
PRIMER_RIGHT_372_SELF_END=0.00
PRIMER_LEFT_372_END_STABILITY=8.5000
PRIMER_RIGHT_372_END_STABILITY=10.6000
PRIMER_PAIR_372_COMPL_ANY=5.00
PRIMER_PAIR_372_COMPL_END=0.00
PRIMER_PAIR_372_PRODUCT_SIZE=100
PRIMER_PAIR_372_PRODUCT_TM=79.7
PRIMER_PAIR_373_PENALTY=6.090765
PRIMER_LEFT_373_PENALTY=3.598066
PRIMER_RIGHT_373_PENALTY=2.492700
PRIMER_LEFT_373_SEQUENCE=TATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_373_SEQUENCE=AGTTCTGGATGGTCACTGGTT
PRIMER_LEFT_373=413,22
PRIMER_RIGHT_373=488,21
PRIMER_LEFT_373_TM=58.402
PRIMER_RIGHT_373_TM=58.507
PRIMER_LEFT_373_GC_PERCENT=45.455
PRIMER_RIGHT_373_GC_PERCENT=47.619
PRIMER_LEFT_373_SELF_ANY=5.00
PRIMER_RIGHT_373_SELF_ANY=3.00
PRIMER_LEFT_373_SELF_END=1.00
PRIMER_RIGHT_373_SELF_END=0.00
PRIMER_LEFT_373_END_STABILITY=9.7000
PRIMER_RIGHT_373_END_STABILITY=8.2000
PRIMER_PAIR_373_COMPL_ANY=4.00
PRIMER_PAIR_373_COMPL_END=2.00
PRIMER_PAIR_373_PRODUCT_SIZE=76
PRIMER_PAIR_373_PRODUCT_TM=71.4
PRIMER_PAIR_374_PENALTY=6.102066
PRIMER_LEFT_374_PENALTY=3.449941
PRIMER_RIGHT_374_PENALTY=2.652125
PRIMER_LEFT_374_SEQUENCE=TAGAAGCCAACCAACCAGTGAC
PRIMER_RIGHT_374_SEQUENCE=AGCAGCGGTAGGGAATCA
PRIMER_LEFT_374=456,22
PRIMER_RIGHT_374=551,18
PRIMER_LEFT_374_TM=61.450
PRIMER_RIGHT_374_TM=59.348
PRIMER_LEFT_374_GC_PERCENT=50.000
PRIMER_RIGHT_374_GC_PERCENT=55.556
PRIMER_LEFT_374_SELF_ANY=3.00
PRIMER_RIGHT_374_SELF_ANY=3.00
PRIMER_LEFT_374_SELF_END=3.00
PRIMER_RIGHT_374_SELF_END=1.00
PRIMER_LEFT_374_END_STABILITY=6.1000
PRIMER_RIGHT_374_END_STABILITY=6.9000
PRIMER_PAIR_374_COMPL_ANY=4.00
PRIMER_PAIR_374_COMPL_END=2.00
PRIMER_PAIR_374_PRODUCT_SIZE=96
PRIMER_PAIR_374_PRODUCT_TM=77.1
PRIMER_PAIR_375_PENALTY=6.109880
PRIMER_LEFT_375_PENALTY=3.007677
PRIMER_RIGHT_375_PENALTY=3.102202
PRIMER_LEFT_375_SEQUENCE=TCTTACACCAGGAGAGGATGGA
PRIMER_RIGHT_375_SEQUENCE=CACTGCATGTCTCTTTGGC
PRIMER_LEFT_375=603,22
PRIMER_RIGHT_375=677,19
PRIMER_LEFT_375_TM=61.008
PRIMER_RIGHT_375_TM=57.898
PRIMER_LEFT_375_GC_PERCENT=50.000
PRIMER_RIGHT_375_GC_PERCENT=52.632
PRIMER_LEFT_375_SELF_ANY=3.00
PRIMER_RIGHT_375_SELF_ANY=4.00
PRIMER_LEFT_375_SELF_END=0.00
PRIMER_RIGHT_375_SELF_END=2.00
PRIMER_LEFT_375_END_STABILITY=8.1000
PRIMER_RIGHT_375_END_STABILITY=10.0000
PRIMER_PAIR_375_COMPL_ANY=4.00
PRIMER_PAIR_375_COMPL_END=3.00
PRIMER_PAIR_375_PRODUCT_SIZE=75
PRIMER_PAIR_375_PRODUCT_TM=72.7
PRIMER_PAIR_376_PENALTY=6.118454
PRIMER_LEFT_376_PENALTY=1.604227
PRIMER_RIGHT_376_PENALTY=4.514227
PRIMER_LEFT_376_SEQUENCE=GGGATTCAGATCCATCAGGG
PRIMER_RIGHT_376_SEQUENCE=CAGTTCAGGGTAGACTTCTTGGC
PRIMER_LEFT_376=354,20
PRIMER_RIGHT_376=439,23
PRIMER_LEFT_376_TM=61.604
PRIMER_RIGHT_376_TM=61.514
PRIMER_LEFT_376_GC_PERCENT=55.000
PRIMER_RIGHT_376_GC_PERCENT=52.174
PRIMER_LEFT_376_SELF_ANY=5.00
PRIMER_RIGHT_376_SELF_ANY=4.00
PRIMER_LEFT_376_SELF_END=3.00
PRIMER_RIGHT_376_SELF_END=2.00
PRIMER_LEFT_376_END_STABILITY=9.7000
PRIMER_RIGHT_376_END_STABILITY=10.0000
PRIMER_PAIR_376_COMPL_ANY=3.00
PRIMER_PAIR_376_COMPL_END=1.00
PRIMER_PAIR_376_PRODUCT_SIZE=86
PRIMER_PAIR_376_PRODUCT_TM=72.9
PRIMER_PAIR_377_PENALTY=6.123894
PRIMER_LEFT_377_PENALTY=3.341555
PRIMER_RIGHT_377_PENALTY=2.782339
PRIMER_LEFT_377_SEQUENCE=TGTGGTAGAAGCCAACCAA
PRIMER_RIGHT_377_SEQUENCE=GCGGTAGGGAATCACAAAGTG
PRIMER_LEFT_377=451,19
PRIMER_RIGHT_377=547,21
PRIMER_LEFT_377_TM=57.658
PRIMER_RIGHT_377_TM=61.782
PRIMER_LEFT_377_GC_PERCENT=47.368
PRIMER_RIGHT_377_GC_PERCENT=52.381
PRIMER_LEFT_377_SELF_ANY=4.00
PRIMER_RIGHT_377_SELF_ANY=3.00
PRIMER_LEFT_377_SELF_END=2.00
PRIMER_RIGHT_377_SELF_END=3.00
PRIMER_LEFT_377_END_STABILITY=8.2000
PRIMER_RIGHT_377_END_STABILITY=6.7000
PRIMER_PAIR_377_COMPL_ANY=4.00
PRIMER_PAIR_377_COMPL_END=1.00
PRIMER_PAIR_377_PRODUCT_SIZE=97
PRIMER_PAIR_377_PRODUCT_TM=77.4
PRIMER_PAIR_378_PENALTY=6.129218
PRIMER_LEFT_378_PENALTY=2.896506
PRIMER_RIGHT_378_PENALTY=3.232712
PRIMER_LEFT_378_SEQUENCE=CTTTGTGATTCCCTACCGCTG
PRIMER_RIGHT_378_SEQUENCE=TCCTGGTGTAAGAATTTGCACT
PRIMER_LEFT_378=529,21
PRIMER_RIGHT_378=615,22
PRIMER_LEFT_378_TM=61.897
PRIMER_RIGHT_378_TM=58.767
PRIMER_LEFT_378_GC_PERCENT=52.381
PRIMER_RIGHT_378_GC_PERCENT=40.909
PRIMER_LEFT_378_SELF_ANY=3.00
PRIMER_RIGHT_378_SELF_ANY=5.00
PRIMER_LEFT_378_SELF_END=2.00
PRIMER_RIGHT_378_SELF_END=3.00
PRIMER_LEFT_378_END_STABILITY=10.2000
PRIMER_RIGHT_378_END_STABILITY=7.9000
PRIMER_PAIR_378_COMPL_ANY=4.00
PRIMER_PAIR_378_COMPL_END=0.00
PRIMER_PAIR_378_PRODUCT_SIZE=87
PRIMER_PAIR_378_PRODUCT_TM=71.9
PRIMER_PAIR_379_PENALTY=6.147891
PRIMER_LEFT_379_PENALTY=2.298898
PRIMER_RIGHT_379_PENALTY=3.848993
PRIMER_LEFT_379_SEQUENCE=GAAACTCATCTTCACTGGCACA
PRIMER_RIGHT_379_SEQUENCE=ACATGCCGTAGTCATGCAAGTT
PRIMER_LEFT_379=632,22
PRIMER_RIGHT_379=710,22
PRIMER_LEFT_379_TM=60.299
PRIMER_RIGHT_379_TM=61.849
PRIMER_LEFT_379_GC_PERCENT=45.455
PRIMER_RIGHT_379_GC_PERCENT=45.455
PRIMER_LEFT_379_SELF_ANY=3.00
PRIMER_RIGHT_379_SELF_ANY=6.00
PRIMER_LEFT_379_SELF_END=2.00
PRIMER_RIGHT_379_SELF_END=3.00
PRIMER_LEFT_379_END_STABILITY=8.2000
PRIMER_RIGHT_379_END_STABILITY=6.7000
PRIMER_PAIR_379_COMPL_ANY=5.00
PRIMER_PAIR_379_COMPL_END=2.00
PRIMER_PAIR_379_PRODUCT_SIZE=79
PRIMER_PAIR_379_PRODUCT_TM=72.5
PRIMER_PAIR_380_PENALTY=6.163108
PRIMER_LEFT_380_PENALTY=1.648882
PRIMER_RIGHT_380_PENALTY=4.514227
PRIMER_LEFT_380_SEQUENCE=AAGTGGGATTCAGATCCATCA
PRIMER_RIGHT_380_SEQUENCE=GCAGTTCAGGGTAGACTTCTTGG
PRIMER_LEFT_380=350,21
PRIMER_RIGHT_380=440,23
PRIMER_LEFT_380_TM=59.351
PRIMER_RIGHT_380_TM=61.514
PRIMER_LEFT_380_GC_PERCENT=42.857
PRIMER_RIGHT_380_GC_PERCENT=52.174
PRIMER_LEFT_380_SELF_ANY=5.00
PRIMER_RIGHT_380_SELF_ANY=4.00
PRIMER_LEFT_380_SELF_END=2.00
PRIMER_RIGHT_380_SELF_END=0.00
PRIMER_LEFT_380_END_STABILITY=6.9000
PRIMER_RIGHT_380_END_STABILITY=8.5000
PRIMER_PAIR_380_COMPL_ANY=4.00
PRIMER_PAIR_380_COMPL_END=2.00
PRIMER_PAIR_380_PRODUCT_SIZE=91
PRIMER_PAIR_380_PRODUCT_TM=73.1
PRIMER_PAIR_381_PENALTY=6.172759
PRIMER_LEFT_381_PENALTY=3.503107
PRIMER_RIGHT_381_PENALTY=2.669652
PRIMER_LEFT_381_SEQUENCE=GCTGGAGGTACCCACTGAT
PRIMER_RIGHT_381_SEQUENCE=GTTCAGTCTGCCACAGAACA
PRIMER_LEFT_381=250,19
PRIMER_RIGHT_381=325,20
PRIMER_LEFT_381_TM=57.497
PRIMER_RIGHT_381_TM=57.330
PRIMER_LEFT_381_GC_PERCENT=57.895
PRIMER_RIGHT_381_GC_PERCENT=50.000
PRIMER_LEFT_381_SELF_ANY=6.00
PRIMER_RIGHT_381_SELF_ANY=6.00
PRIMER_LEFT_381_SELF_END=2.00
PRIMER_RIGHT_381_SELF_END=2.00
PRIMER_LEFT_381_END_STABILITY=6.6000
PRIMER_RIGHT_381_END_STABILITY=6.7000
PRIMER_PAIR_381_COMPL_ANY=5.00
PRIMER_PAIR_381_COMPL_END=2.00
PRIMER_PAIR_381_PRODUCT_SIZE=76
PRIMER_PAIR_381_PRODUCT_TM=75.2
PRIMER_PAIR_382_PENALTY=6.207793
PRIMER_LEFT_382_PENALTY=2.358800
PRIMER_RIGHT_382_PENALTY=3.848993
PRIMER_LEFT_382_SEQUENCE=TGCGAAACTCATCTTCACTGG
PRIMER_RIGHT_382_SEQUENCE=AACATGCCGTAGTCATGCAAGT
PRIMER_LEFT_382=629,21
PRIMER_RIGHT_382=711,22
PRIMER_LEFT_382_TM=61.359
PRIMER_RIGHT_382_TM=61.849
PRIMER_LEFT_382_GC_PERCENT=47.619
PRIMER_RIGHT_382_GC_PERCENT=45.455
PRIMER_LEFT_382_SELF_ANY=3.00
PRIMER_RIGHT_382_SELF_ANY=6.00
PRIMER_LEFT_382_SELF_END=1.00
PRIMER_RIGHT_382_SELF_END=3.00
PRIMER_LEFT_382_END_STABILITY=7.9000
PRIMER_RIGHT_382_END_STABILITY=6.7000
PRIMER_PAIR_382_COMPL_ANY=4.00
PRIMER_PAIR_382_COMPL_END=3.00
PRIMER_PAIR_382_PRODUCT_SIZE=83
PRIMER_PAIR_382_PRODUCT_TM=72.9
PRIMER_PAIR_383_PENALTY=6.214797
PRIMER_LEFT_383_PENALTY=3.132848
PRIMER_RIGHT_383_PENALTY=3.081950
PRIMER_LEFT_383_SEQUENCE=TTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_383_SEQUENCE=CACCAGTTCTGGATGGTCA
PRIMER_LEFT_383=415,22
PRIMER_RIGHT_383=492,19
PRIMER_LEFT_383_TM=61.133
PRIMER_RIGHT_383_TM=57.918
PRIMER_LEFT_383_GC_PERCENT=45.455
PRIMER_RIGHT_383_GC_PERCENT=52.632
PRIMER_LEFT_383_SELF_ANY=5.00
PRIMER_RIGHT_383_SELF_ANY=6.00
PRIMER_LEFT_383_SELF_END=0.00
PRIMER_RIGHT_383_SELF_END=2.00
PRIMER_LEFT_383_END_STABILITY=7.0000
PRIMER_RIGHT_383_END_STABILITY=7.9000
PRIMER_PAIR_383_COMPL_ANY=4.00
PRIMER_PAIR_383_COMPL_END=3.00
PRIMER_PAIR_383_PRODUCT_SIZE=78
PRIMER_PAIR_383_PRODUCT_TM=72.7
PRIMER_PAIR_384_PENALTY=6.218841
PRIMER_LEFT_384_PENALTY=4.341090
PRIMER_RIGHT_384_PENALTY=1.877751
PRIMER_LEFT_384_SEQUENCE=CCAAAGAGACATGCAGTGAGAAG
PRIMER_RIGHT_384_SEQUENCE=ACACACAAACTCTACCCCTCG
PRIMER_LEFT_384=660,23
PRIMER_RIGHT_384=757,21
PRIMER_LEFT_384_TM=61.341
PRIMER_RIGHT_384_TM=59.122
PRIMER_LEFT_384_GC_PERCENT=47.826
PRIMER_RIGHT_384_GC_PERCENT=52.381
PRIMER_LEFT_384_SELF_ANY=4.00
PRIMER_RIGHT_384_SELF_ANY=2.00
PRIMER_LEFT_384_SELF_END=0.00
PRIMER_RIGHT_384_SELF_END=2.00
PRIMER_LEFT_384_END_STABILITY=6.7000
PRIMER_RIGHT_384_END_STABILITY=9.9000
PRIMER_PAIR_384_COMPL_ANY=4.00
PRIMER_PAIR_384_COMPL_END=1.00
PRIMER_PAIR_384_PRODUCT_SIZE=98
PRIMER_PAIR_384_PRODUCT_TM=74.3
PRIMER_PAIR_385_PENALTY=6.222255
PRIMER_LEFT_385_PENALTY=4.253992
PRIMER_RIGHT_385_PENALTY=1.968263
PRIMER_LEFT_385_SEQUENCE=TGCAGTATTGCCAAGAAGTCTA
PRIMER_RIGHT_385_SEQUENCE=TCTGGATGGTCACTGGTTGG
PRIMER_LEFT_385=408,22
PRIMER_RIGHT_385=485,20
PRIMER_LEFT_385_TM=57.746
PRIMER_RIGHT_385_TM=61.968
PRIMER_LEFT_385_GC_PERCENT=40.909
PRIMER_RIGHT_385_GC_PERCENT=55.000
PRIMER_LEFT_385_SELF_ANY=6.00
PRIMER_RIGHT_385_SELF_ANY=3.00
PRIMER_LEFT_385_SELF_END=2.00
PRIMER_RIGHT_385_SELF_END=0.00
PRIMER_LEFT_385_END_STABILITY=5.4000
PRIMER_RIGHT_385_END_STABILITY=8.2000
PRIMER_PAIR_385_COMPL_ANY=5.00
PRIMER_PAIR_385_COMPL_END=3.00
PRIMER_PAIR_385_PRODUCT_SIZE=78
PRIMER_PAIR_385_PRODUCT_TM=72.2
PRIMER_PAIR_386_PENALTY=6.230821
PRIMER_LEFT_386_PENALTY=4.044290
PRIMER_RIGHT_386_PENALTY=2.186531
PRIMER_LEFT_386_SEQUENCE=ATTGCCATGTTCTGTGGC
PRIMER_RIGHT_386_SEQUENCE=CAATGCAGGTTTTGGTCCCT
PRIMER_LEFT_386=299,18
PRIMER_RIGHT_386=389,20
PRIMER_LEFT_386_TM=57.956
PRIMER_RIGHT_386_TM=62.187
PRIMER_LEFT_386_GC_PERCENT=50.000
PRIMER_RIGHT_386_GC_PERCENT=50.000
PRIMER_LEFT_386_SELF_ANY=4.00
PRIMER_RIGHT_386_SELF_ANY=4.00
PRIMER_LEFT_386_SELF_END=2.00
PRIMER_RIGHT_386_SELF_END=0.00
PRIMER_LEFT_386_END_STABILITY=9.4000
PRIMER_RIGHT_386_END_STABILITY=9.4000
PRIMER_PAIR_386_COMPL_ANY=4.00
PRIMER_PAIR_386_COMPL_END=0.00
PRIMER_PAIR_386_PRODUCT_SIZE=91
PRIMER_PAIR_386_PRODUCT_TM=72.7
PRIMER_PAIR_387_PENALTY=6.235964
PRIMER_LEFT_387_PENALTY=3.668658
PRIMER_RIGHT_387_PENALTY=2.567306
PRIMER_LEFT_387_SEQUENCE=ATCACCAATGTGGTAGAAGCCA
PRIMER_RIGHT_387_SEQUENCE=GGGTCTTGCACTGCTTGC
PRIMER_LEFT_387=443,22
PRIMER_RIGHT_387=521,18
PRIMER_LEFT_387_TM=61.669
PRIMER_RIGHT_387_TM=60.567
PRIMER_LEFT_387_GC_PERCENT=45.455
PRIMER_RIGHT_387_GC_PERCENT=61.111
PRIMER_LEFT_387_SELF_ANY=6.00
PRIMER_RIGHT_387_SELF_ANY=5.00
PRIMER_LEFT_387_SELF_END=1.00
PRIMER_RIGHT_387_SELF_END=2.00
PRIMER_LEFT_387_END_STABILITY=9.7000
PRIMER_RIGHT_387_END_STABILITY=8.5000
PRIMER_PAIR_387_COMPL_ANY=4.00
PRIMER_PAIR_387_COMPL_END=2.00
PRIMER_PAIR_387_PRODUCT_SIZE=79
PRIMER_PAIR_387_PRODUCT_TM=75.7
PRIMER_PAIR_388_PENALTY=6.247948
PRIMER_LEFT_388_PENALTY=2.022189
PRIMER_RIGHT_388_PENALTY=4.225759
PRIMER_LEFT_388_SEQUENCE=CACCAGGAGAGGATGGATG
PRIMER_RIGHT_388_SEQUENCE=CATGCAAGTTGGTACTCTTCTCAC
PRIMER_LEFT_388=608,19
PRIMER_RIGHT_388=698,24
PRIMER_LEFT_388_TM=58.978
PRIMER_RIGHT_388_TM=60.226
PRIMER_LEFT_388_GC_PERCENT=57.895
PRIMER_RIGHT_388_GC_PERCENT=45.833
PRIMER_LEFT_388_SELF_ANY=3.00
PRIMER_RIGHT_388_SELF_ANY=5.00
PRIMER_LEFT_388_SELF_END=0.00
PRIMER_RIGHT_388_SELF_END=0.00
PRIMER_LEFT_388_END_STABILITY=8.1000
PRIMER_RIGHT_388_END_STABILITY=6.4000
PRIMER_PAIR_388_COMPL_ANY=5.00
PRIMER_PAIR_388_COMPL_END=1.00
PRIMER_PAIR_388_PRODUCT_SIZE=91
PRIMER_PAIR_388_PRODUCT_TM=74.0
PRIMER_PAIR_389_PENALTY=6.263236
PRIMER_LEFT_389_PENALTY=3.449941
PRIMER_RIGHT_389_PENALTY=2.813295
PRIMER_LEFT_389_SEQUENCE=GTAGAAGCCAACCAACCAGTGA
PRIMER_RIGHT_389_SEQUENCE=AATCACAAAGTGGGGATGG
PRIMER_LEFT_389=455,22
PRIMER_RIGHT_389=538,19
PRIMER_LEFT_389_TM=61.450
PRIMER_RIGHT_389_TM=58.187
PRIMER_LEFT_389_GC_PERCENT=50.000
PRIMER_RIGHT_389_GC_PERCENT=47.368
PRIMER_LEFT_389_SELF_ANY=3.00
PRIMER_RIGHT_389_SELF_ANY=4.00
PRIMER_LEFT_389_SELF_END=1.00
PRIMER_RIGHT_389_SELF_END=0.00
PRIMER_LEFT_389_END_STABILITY=6.4000
PRIMER_RIGHT_389_END_STABILITY=8.1000
PRIMER_PAIR_389_COMPL_ANY=4.00
PRIMER_PAIR_389_COMPL_END=3.00
PRIMER_PAIR_389_PRODUCT_SIZE=84
PRIMER_PAIR_389_PRODUCT_TM=75.7
PRIMER_PAIR_390_PENALTY=6.276752
PRIMER_LEFT_390_PENALTY=3.657669
PRIMER_RIGHT_390_PENALTY=2.619082
PRIMER_LEFT_390_SEQUENCE=GCAGTATTGCCAAGAAGTCTACC
PRIMER_RIGHT_390_SEQUENCE=GTTCTGGATGGTCACTGGTT
PRIMER_LEFT_390=409,23
PRIMER_RIGHT_390=487,20
PRIMER_LEFT_390_TM=59.342
PRIMER_RIGHT_390_TM=57.381
PRIMER_LEFT_390_GC_PERCENT=47.826
PRIMER_RIGHT_390_GC_PERCENT=50.000
PRIMER_LEFT_390_SELF_ANY=6.00
PRIMER_RIGHT_390_SELF_ANY=3.00
PRIMER_LEFT_390_SELF_END=1.00
PRIMER_RIGHT_390_SELF_END=0.00
PRIMER_LEFT_390_END_STABILITY=6.9000
PRIMER_RIGHT_390_END_STABILITY=8.2000
PRIMER_PAIR_390_COMPL_ANY=4.00
PRIMER_PAIR_390_COMPL_END=3.00
PRIMER_PAIR_390_PRODUCT_SIZE=79
PRIMER_PAIR_390_PRODUCT_TM=72.5
PRIMER_PAIR_391_PENALTY=6.289984
PRIMER_LEFT_391_PENALTY=3.579934
PRIMER_RIGHT_391_PENALTY=2.710050
PRIMER_LEFT_391_SEQUENCE=TCCAGAACTGGTGCAAGC
PRIMER_RIGHT_391_SEQUENCE=AACTCACCAACTAAGCAGCGG
PRIMER_LEFT_391=480,18
PRIMER_RIGHT_391=564,21
PRIMER_LEFT_391_TM=58.420
PRIMER_RIGHT_391_TM=61.710
PRIMER_LEFT_391_GC_PERCENT=55.556
PRIMER_RIGHT_391_GC_PERCENT=52.381
PRIMER_LEFT_391_SELF_ANY=6.00
PRIMER_RIGHT_391_SELF_ANY=3.00
PRIMER_LEFT_391_SELF_END=2.00
PRIMER_RIGHT_391_SELF_END=2.00
PRIMER_LEFT_391_END_STABILITY=8.5000
PRIMER_RIGHT_391_END_STABILITY=11.4000
PRIMER_PAIR_391_COMPL_ANY=5.00
PRIMER_PAIR_391_COMPL_END=1.00
PRIMER_PAIR_391_PRODUCT_SIZE=85
PRIMER_PAIR_391_PRODUCT_TM=76.0
PRIMER_PAIR_392_PENALTY=6.303985
PRIMER_LEFT_392_PENALTY=3.033298
PRIMER_RIGHT_392_PENALTY=3.270687
PRIMER_LEFT_392_SEQUENCE=TGTAAGTGATGCCCTTCTCGTT
PRIMER_RIGHT_392_SEQUENCE=ACGGTGTGCCAGTGAAGA
PRIMER_LEFT_392=565,22
PRIMER_RIGHT_392=657,18
PRIMER_LEFT_392_TM=61.033
PRIMER_RIGHT_392_TM=58.729
PRIMER_LEFT_392_GC_PERCENT=45.455
PRIMER_RIGHT_392_GC_PERCENT=55.556
PRIMER_LEFT_392_SELF_ANY=4.00
PRIMER_RIGHT_392_SELF_ANY=3.00
PRIMER_LEFT_392_SELF_END=2.00
PRIMER_RIGHT_392_SELF_END=0.00
PRIMER_LEFT_392_END_STABILITY=8.4000
PRIMER_RIGHT_392_END_STABILITY=6.7000
PRIMER_PAIR_392_COMPL_ANY=4.00
PRIMER_PAIR_392_COMPL_END=2.00
PRIMER_PAIR_392_PRODUCT_SIZE=93
PRIMER_PAIR_392_PRODUCT_TM=72.8
PRIMER_PAIR_393_PENALTY=6.303985
PRIMER_LEFT_393_PENALTY=3.033298
PRIMER_RIGHT_393_PENALTY=3.270687
PRIMER_LEFT_393_SEQUENCE=TTGTAAGTGATGCCCTTCTCGT
PRIMER_RIGHT_393_SEQUENCE=GACGGTGTGCCAGTGAAG
PRIMER_LEFT_393=564,22
PRIMER_RIGHT_393=658,18
PRIMER_LEFT_393_TM=61.033
PRIMER_RIGHT_393_TM=58.729
PRIMER_LEFT_393_GC_PERCENT=45.455
PRIMER_RIGHT_393_GC_PERCENT=61.111
PRIMER_LEFT_393_SELF_ANY=4.00
PRIMER_RIGHT_393_SELF_ANY=3.00
PRIMER_LEFT_393_SELF_END=2.00
PRIMER_RIGHT_393_SELF_END=0.00
PRIMER_LEFT_393_END_STABILITY=8.1000
PRIMER_RIGHT_393_END_STABILITY=7.0000
PRIMER_PAIR_393_COMPL_ANY=4.00
PRIMER_PAIR_393_COMPL_END=3.00
PRIMER_PAIR_393_PRODUCT_SIZE=95
PRIMER_PAIR_393_PRODUCT_TM=73.0
PRIMER_PAIR_394_PENALTY=6.314354
PRIMER_LEFT_394_PENALTY=3.057917
PRIMER_RIGHT_394_PENALTY=3.256437
PRIMER_LEFT_394_SEQUENCE=CCTACCGCTGCTTAGTTGGTG
PRIMER_RIGHT_394_SEQUENCE=CATCCATCCTCTCCTGGTGTAA
PRIMER_LEFT_394=540,21
PRIMER_RIGHT_394=626,22
PRIMER_LEFT_394_TM=62.058
PRIMER_RIGHT_394_TM=61.256
PRIMER_LEFT_394_GC_PERCENT=57.143
PRIMER_RIGHT_394_GC_PERCENT=50.000
PRIMER_LEFT_394_SELF_ANY=3.00
PRIMER_RIGHT_394_SELF_ANY=3.00
PRIMER_LEFT_394_SELF_END=0.00
PRIMER_RIGHT_394_SELF_END=1.00
PRIMER_LEFT_394_END_STABILITY=8.2000
PRIMER_RIGHT_394_END_STABILITY=6.0000
PRIMER_PAIR_394_COMPL_ANY=3.00
PRIMER_PAIR_394_COMPL_END=0.00
PRIMER_PAIR_394_PRODUCT_SIZE=87
PRIMER_PAIR_394_PRODUCT_TM=72.8
PRIMER_PAIR_395_PENALTY=6.335765
PRIMER_LEFT_395_PENALTY=2.358800
PRIMER_RIGHT_395_PENALTY=3.976964
PRIMER_LEFT_395_SEQUENCE=CGAAACTCATCTTCACTGGCA
PRIMER_RIGHT_395_SEQUENCE=CAACATGCCGTAGTCATGCAA
PRIMER_LEFT_395=631,21
PRIMER_RIGHT_395=712,21
PRIMER_LEFT_395_TM=61.359
PRIMER_RIGHT_395_TM=62.977
PRIMER_LEFT_395_GC_PERCENT=47.619
PRIMER_RIGHT_395_GC_PERCENT=47.619
PRIMER_LEFT_395_SELF_ANY=3.00
PRIMER_RIGHT_395_SELF_ANY=6.00
PRIMER_LEFT_395_SELF_END=3.00
PRIMER_RIGHT_395_SELF_END=2.00
PRIMER_LEFT_395_END_STABILITY=10.0000
PRIMER_RIGHT_395_END_STABILITY=8.8000
PRIMER_PAIR_395_COMPL_ANY=4.00
PRIMER_PAIR_395_COMPL_END=3.00
PRIMER_PAIR_395_PRODUCT_SIZE=82
PRIMER_PAIR_395_PRODUCT_TM=73.1
PRIMER_PAIR_396_PENALTY=6.337271
PRIMER_LEFT_396_PENALTY=4.354763
PRIMER_RIGHT_396_PENALTY=1.982507
PRIMER_LEFT_396_SEQUENCE=ATCCTGCAGTATTGCCAAGAAGT
PRIMER_RIGHT_396_SEQUENCE=GGTCACTGGTTGGTTGGCT
PRIMER_LEFT_396=404,23
PRIMER_RIGHT_396=478,19
PRIMER_LEFT_396_TM=61.355
PRIMER_RIGHT_396_TM=60.983
PRIMER_LEFT_396_GC_PERCENT=43.478
PRIMER_RIGHT_396_GC_PERCENT=57.895
PRIMER_LEFT_396_SELF_ANY=8.00
PRIMER_RIGHT_396_SELF_ANY=3.00
PRIMER_LEFT_396_SELF_END=2.00
PRIMER_RIGHT_396_SELF_END=0.00
PRIMER_LEFT_396_END_STABILITY=6.4000
PRIMER_RIGHT_396_END_STABILITY=9.7000
PRIMER_PAIR_396_COMPL_ANY=5.00
PRIMER_PAIR_396_COMPL_END=1.00
PRIMER_PAIR_396_PRODUCT_SIZE=75
PRIMER_PAIR_396_PRODUCT_TM=72.1
PRIMER_PAIR_397_PENALTY=6.339262
PRIMER_LEFT_397_PENALTY=1.676675
PRIMER_RIGHT_397_PENALTY=4.662587
PRIMER_LEFT_397_SEQUENCE=GGATTCAGATCCATCAGGGAC
PRIMER_RIGHT_397_SEQUENCE=GATCTGCAGTTCAGGGTAGACTTC
PRIMER_LEFT_397=355,21
PRIMER_RIGHT_397=445,24
PRIMER_LEFT_397_TM=60.677
PRIMER_RIGHT_397_TM=60.663
PRIMER_LEFT_397_GC_PERCENT=52.381
PRIMER_RIGHT_397_GC_PERCENT=50.000
PRIMER_LEFT_397_SELF_ANY=5.00
PRIMER_RIGHT_397_SELF_ANY=8.00
PRIMER_LEFT_397_SELF_END=3.00
PRIMER_RIGHT_397_SELF_END=3.00
PRIMER_LEFT_397_END_STABILITY=9.1000
PRIMER_RIGHT_397_END_STABILITY=6.4000
PRIMER_PAIR_397_COMPL_ANY=6.00
PRIMER_PAIR_397_COMPL_END=1.00
PRIMER_PAIR_397_PRODUCT_SIZE=91
PRIMER_PAIR_397_PRODUCT_TM=73.1
PRIMER_PAIR_398_PENALTY=6.354506
PRIMER_LEFT_398_PENALTY=3.776889
PRIMER_RIGHT_398_PENALTY=2.577616
PRIMER_LEFT_398_SEQUENCE=CGCAAGCAGTGCAAGACC
PRIMER_RIGHT_398_SEQUENCE=AACGAGAAGGGCATCACTTA
PRIMER_LEFT_398=503,18
PRIMER_RIGHT_398=586,20
PRIMER_LEFT_398_TM=61.777
PRIMER_RIGHT_398_TM=57.422
PRIMER_LEFT_398_GC_PERCENT=61.111
PRIMER_RIGHT_398_GC_PERCENT=45.000
PRIMER_LEFT_398_SELF_ANY=5.00
PRIMER_RIGHT_398_SELF_ANY=4.00
PRIMER_LEFT_398_SELF_END=0.00
PRIMER_RIGHT_398_SELF_END=2.00
PRIMER_LEFT_398_END_STABILITY=7.6000
PRIMER_RIGHT_398_END_STABILITY=5.7000
PRIMER_PAIR_398_COMPL_ANY=4.00
PRIMER_PAIR_398_COMPL_END=2.00
PRIMER_PAIR_398_PRODUCT_SIZE=84
PRIMER_PAIR_398_PRODUCT_TM=73.7
PRIMER_PAIR_399_PENALTY=6.367331
PRIMER_LEFT_399_PENALTY=3.070814
PRIMER_RIGHT_399_PENALTY=3.296517
PRIMER_LEFT_399_SEQUENCE=GATTCCCTACCGCTGCTTA
PRIMER_RIGHT_399_SEQUENCE=CTCCTGGTGTAAGAATTTGCACT
PRIMER_LEFT_399=535,19
PRIMER_RIGHT_399=616,23
PRIMER_LEFT_399_TM=57.929
PRIMER_RIGHT_399_TM=59.703
PRIMER_LEFT_399_GC_PERCENT=52.632
PRIMER_RIGHT_399_GC_PERCENT=43.478
PRIMER_LEFT_399_SELF_ANY=3.00
PRIMER_RIGHT_399_SELF_ANY=5.00
PRIMER_LEFT_399_SELF_END=2.00
PRIMER_RIGHT_399_SELF_END=3.00
PRIMER_LEFT_399_END_STABILITY=7.5000
PRIMER_RIGHT_399_END_STABILITY=7.9000
PRIMER_PAIR_399_COMPL_ANY=4.00
PRIMER_PAIR_399_COMPL_END=1.00
PRIMER_PAIR_399_PRODUCT_SIZE=82
PRIMER_PAIR_399_PRODUCT_TM=72.1
PRIMER_PAIR_400_PENALTY=6.369600
PRIMER_LEFT_400_PENALTY=3.925274
PRIMER_RIGHT_400_PENALTY=2.444325
PRIMER_LEFT_400_SEQUENCE=GACCAAAACCTGCATTGATAC
PRIMER_RIGHT_400_SEQUENCE=TGGTTGGTTGGCTTCTACC
PRIMER_LEFT_400=373,21
PRIMER_RIGHT_400=472,19
PRIMER_LEFT_400_TM=57.075
PRIMER_RIGHT_400_TM=58.556
PRIMER_LEFT_400_GC_PERCENT=42.857
PRIMER_RIGHT_400_GC_PERCENT=52.632
PRIMER_LEFT_400_SELF_ANY=4.00
PRIMER_RIGHT_400_SELF_ANY=3.00
PRIMER_LEFT_400_SELF_END=2.00
PRIMER_RIGHT_400_SELF_END=2.00
PRIMER_LEFT_400_END_STABILITY=5.3000
PRIMER_RIGHT_400_END_STABILITY=6.9000
PRIMER_PAIR_400_COMPL_ANY=5.00
PRIMER_PAIR_400_COMPL_END=1.00
PRIMER_PAIR_400_PRODUCT_SIZE=100
PRIMER_PAIR_400_PRODUCT_TM=73.6
PRIMER_PAIR_401_PENALTY=6.394823
PRIMER_LEFT_401_PENALTY=3.612510
PRIMER_RIGHT_401_PENALTY=2.782312
PRIMER_LEFT_401_SEQUENCE=CAGAACTGGTGCAAGCGG
PRIMER_RIGHT_401_SEQUENCE=GGGCATCACTTACAAACTCACC
PRIMER_LEFT_401=482,18
PRIMER_RIGHT_401=578,22
PRIMER_LEFT_401_TM=61.613
PRIMER_RIGHT_401_TM=60.782
PRIMER_LEFT_401_GC_PERCENT=61.111
PRIMER_RIGHT_401_GC_PERCENT=50.000
PRIMER_LEFT_401_SELF_ANY=4.00
PRIMER_RIGHT_401_SELF_ANY=2.00
PRIMER_LEFT_401_SELF_END=1.00
PRIMER_RIGHT_401_SELF_END=0.00
PRIMER_LEFT_401_END_STABILITY=11.4000
PRIMER_RIGHT_401_END_STABILITY=7.9000
PRIMER_PAIR_401_COMPL_ANY=5.00
PRIMER_PAIR_401_COMPL_END=2.00
PRIMER_PAIR_401_PRODUCT_SIZE=97
PRIMER_PAIR_401_PRODUCT_TM=76.5
PRIMER_PAIR_402_PENALTY=6.403542
PRIMER_LEFT_402_PENALTY=1.543091
PRIMER_RIGHT_402_PENALTY=4.860451
PRIMER_LEFT_402_SEQUENCE=TGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_402_SEQUENCE=TCAGGGTAGACTTCTTGGCAATAC
PRIMER_LEFT_402=337,19
PRIMER_RIGHT_402=435,24
PRIMER_LEFT_402_TM=59.457
PRIMER_RIGHT_402_TM=60.860
PRIMER_LEFT_402_GC_PERCENT=52.632
PRIMER_RIGHT_402_GC_PERCENT=45.833
PRIMER_LEFT_402_SELF_ANY=3.00
PRIMER_RIGHT_402_SELF_ANY=5.00
PRIMER_LEFT_402_SELF_END=0.00
PRIMER_RIGHT_402_SELF_END=3.00
PRIMER_LEFT_402_END_STABILITY=7.9000
PRIMER_RIGHT_402_END_STABILITY=5.6000
PRIMER_PAIR_402_COMPL_ANY=5.00
PRIMER_PAIR_402_COMPL_END=1.00
PRIMER_PAIR_402_PRODUCT_SIZE=99
PRIMER_PAIR_402_PRODUCT_TM=73.7
PRIMER_PAIR_403_PENALTY=6.445098
PRIMER_LEFT_403_PENALTY=3.776889
PRIMER_RIGHT_403_PENALTY=2.668208
PRIMER_LEFT_403_SEQUENCE=CCGCAAGCAGTGCAAGAC
PRIMER_RIGHT_403_SEQUENCE=AACGAGAAGGGCATCACTTAC
PRIMER_LEFT_403=502,18
PRIMER_RIGHT_403=586,21
PRIMER_LEFT_403_TM=61.777
PRIMER_RIGHT_403_TM=58.332
PRIMER_LEFT_403_GC_PERCENT=61.111
PRIMER_RIGHT_403_GC_PERCENT=47.619
PRIMER_LEFT_403_SELF_ANY=5.00
PRIMER_RIGHT_403_SELF_ANY=4.00
PRIMER_LEFT_403_SELF_END=1.00
PRIMER_RIGHT_403_SELF_END=2.00
PRIMER_LEFT_403_END_STABILITY=6.4000
PRIMER_RIGHT_403_END_STABILITY=5.7000
PRIMER_PAIR_403_COMPL_ANY=4.00
PRIMER_PAIR_403_COMPL_END=1.00
PRIMER_PAIR_403_PRODUCT_SIZE=85
PRIMER_PAIR_403_PRODUCT_TM=74.1
PRIMER_PAIR_404_PENALTY=6.445296
PRIMER_LEFT_404_PENALTY=4.021939
PRIMER_RIGHT_404_PENALTY=2.423358
PRIMER_LEFT_404_SEQUENCE=TACCCACTGATGGTAATGCTGG
PRIMER_RIGHT_404_SEQUENCE=TCCCACTTCCCATTCTGGA
PRIMER_LEFT_404=258,22
PRIMER_RIGHT_404=357,19
PRIMER_LEFT_404_TM=62.022
PRIMER_RIGHT_404_TM=61.423
PRIMER_LEFT_404_GC_PERCENT=50.000
PRIMER_RIGHT_404_GC_PERCENT=52.632
PRIMER_LEFT_404_SELF_ANY=5.00
PRIMER_RIGHT_404_SELF_ANY=3.00
PRIMER_LEFT_404_SELF_END=0.00
PRIMER_RIGHT_404_SELF_END=2.00
PRIMER_LEFT_404_END_STABILITY=9.7000
PRIMER_RIGHT_404_END_STABILITY=8.2000
PRIMER_PAIR_404_COMPL_ANY=5.00
PRIMER_PAIR_404_COMPL_END=3.00
PRIMER_PAIR_404_PRODUCT_SIZE=100
PRIMER_PAIR_404_PRODUCT_TM=75.2
PRIMER_PAIR_405_PENALTY=6.445538
PRIMER_LEFT_405_PENALTY=4.381939
PRIMER_RIGHT_405_PENALTY=2.063598
PRIMER_LEFT_405_SEQUENCE=GCATTGATACCAAGGAAGGCAT
PRIMER_RIGHT_405_SEQUENCE=GGTCACTGGTTGGTTGGC
PRIMER_LEFT_405=384,22
PRIMER_RIGHT_405=478,18
PRIMER_LEFT_405_TM=62.382
PRIMER_RIGHT_405_TM=59.936
PRIMER_LEFT_405_GC_PERCENT=45.455
PRIMER_RIGHT_405_GC_PERCENT=61.111
PRIMER_LEFT_405_SELF_ANY=6.00
PRIMER_RIGHT_405_SELF_ANY=3.00
PRIMER_LEFT_405_SELF_END=2.00
PRIMER_RIGHT_405_SELF_END=2.00
PRIMER_LEFT_405_END_STABILITY=9.6000
PRIMER_RIGHT_405_END_STABILITY=10.0000
PRIMER_PAIR_405_COMPL_ANY=5.00
PRIMER_PAIR_405_COMPL_END=1.00
PRIMER_PAIR_405_PRODUCT_SIZE=95
PRIMER_PAIR_405_PRODUCT_TM=73.9
PRIMER_PAIR_406_PENALTY=6.451251
PRIMER_LEFT_406_PENALTY=3.244134
PRIMER_RIGHT_406_PENALTY=3.207117
PRIMER_LEFT_406_SEQUENCE=GCAAGACCCATCCCCACTT
PRIMER_RIGHT_406_SEQUENCE=GAACGAGAAGGGCATCACT
PRIMER_LEFT_406=513,19
PRIMER_RIGHT_406=587,19
PRIMER_LEFT_406_TM=62.244
PRIMER_RIGHT_406_TM=57.793
PRIMER_LEFT_406_GC_PERCENT=57.895
PRIMER_RIGHT_406_GC_PERCENT=52.632
PRIMER_LEFT_406_SELF_ANY=3.00
PRIMER_RIGHT_406_SELF_ANY=2.00
PRIMER_LEFT_406_SELF_END=0.00
PRIMER_RIGHT_406_SELF_END=2.00
PRIMER_LEFT_406_END_STABILITY=6.7000
PRIMER_RIGHT_406_END_STABILITY=6.4000
PRIMER_PAIR_406_COMPL_ANY=4.00
PRIMER_PAIR_406_COMPL_END=0.00
PRIMER_PAIR_406_PRODUCT_SIZE=75
PRIMER_PAIR_406_PRODUCT_TM=72.7
PRIMER_PAIR_407_PENALTY=6.485511
PRIMER_LEFT_407_PENALTY=3.173109
PRIMER_RIGHT_407_PENALTY=3.312402
PRIMER_LEFT_407_SEQUENCE=TGTGATTCCCTACCGCTGC
PRIMER_RIGHT_407_SEQUENCE=AAGAATTTGCACTTGTCAGGAAC
PRIMER_LEFT_407=532,19
PRIMER_RIGHT_407=606,23
PRIMER_LEFT_407_TM=62.173
PRIMER_RIGHT_407_TM=59.688
PRIMER_LEFT_407_GC_PERCENT=57.895
PRIMER_RIGHT_407_GC_PERCENT=39.130
PRIMER_LEFT_407_SELF_ANY=3.00
PRIMER_RIGHT_407_SELF_ANY=4.00
PRIMER_LEFT_407_SELF_END=3.00
PRIMER_RIGHT_407_SELF_END=1.00
PRIMER_LEFT_407_END_STABILITY=9.7000
PRIMER_RIGHT_407_END_STABILITY=7.9000
PRIMER_PAIR_407_COMPL_ANY=4.00
PRIMER_PAIR_407_COMPL_END=3.00
PRIMER_PAIR_407_PRODUCT_SIZE=75
PRIMER_PAIR_407_PRODUCT_TM=70.5
PRIMER_PAIR_408_PENALTY=6.510655
PRIMER_LEFT_408_PENALTY=3.048411
PRIMER_RIGHT_408_PENALTY=3.462244
PRIMER_LEFT_408_SEQUENCE=AAGTGCAAATTCTTACACCAGGA
PRIMER_RIGHT_408_SEQUENCE=CTCTTTGGCGACGGTGTG
PRIMER_LEFT_408=593,23
PRIMER_RIGHT_408=667,18
PRIMER_LEFT_408_TM=60.048
PRIMER_RIGHT_408_TM=61.462
PRIMER_LEFT_408_GC_PERCENT=39.130
PRIMER_RIGHT_408_GC_PERCENT=61.111
PRIMER_LEFT_408_SELF_ANY=5.00
PRIMER_RIGHT_408_SELF_ANY=3.00
PRIMER_LEFT_408_SELF_END=1.00
PRIMER_RIGHT_408_SELF_END=0.00
PRIMER_LEFT_408_END_STABILITY=8.2000
PRIMER_RIGHT_408_END_STABILITY=6.4000
PRIMER_PAIR_408_COMPL_ANY=5.00
PRIMER_PAIR_408_COMPL_END=1.00
PRIMER_PAIR_408_PRODUCT_SIZE=75
PRIMER_PAIR_408_PRODUCT_TM=71.6
PRIMER_PAIR_409_PENALTY=6.516191
PRIMER_LEFT_409_PENALTY=4.599420
PRIMER_RIGHT_409_PENALTY=1.916771
PRIMER_LEFT_409_SEQUENCE=CAAAGAGACATGCAGTGAGAAGAG
PRIMER_RIGHT_409_SEQUENCE=ACACAAACTCTACCCCTCGGA
PRIMER_LEFT_409=661,24
PRIMER_RIGHT_409=755,21
PRIMER_LEFT_409_TM=60.599
PRIMER_RIGHT_409_TM=60.917
PRIMER_LEFT_409_GC_PERCENT=45.833
PRIMER_RIGHT_409_GC_PERCENT=52.381
PRIMER_LEFT_409_SELF_ANY=4.00
PRIMER_RIGHT_409_SELF_ANY=3.00
PRIMER_LEFT_409_SELF_END=0.00
PRIMER_RIGHT_409_SELF_END=3.00
PRIMER_LEFT_409_END_STABILITY=6.7000
PRIMER_RIGHT_409_END_STABILITY=9.9000
PRIMER_PAIR_409_COMPL_ANY=4.00
PRIMER_PAIR_409_COMPL_END=3.00
PRIMER_PAIR_409_PRODUCT_SIZE=95
PRIMER_PAIR_409_PRODUCT_TM=73.9
PRIMER_PAIR_410_PENALTY=6.536925
PRIMER_LEFT_410_PENALTY=4.244134
PRIMER_RIGHT_410_PENALTY=2.292791
PRIMER_LEFT_410_SEQUENCE=GCAGACTGAACATGCACATGAA
PRIMER_RIGHT_410_SEQUENCE=CAATGCAGGTTTTGGTCCC
PRIMER_LEFT_410=315,22
PRIMER_RIGHT_410=389,19
PRIMER_LEFT_410_TM=62.244
PRIMER_RIGHT_410_TM=61.293
PRIMER_LEFT_410_GC_PERCENT=45.455
PRIMER_RIGHT_410_GC_PERCENT=52.632
PRIMER_LEFT_410_SELF_ANY=6.00
PRIMER_RIGHT_410_SELF_ANY=4.00
PRIMER_LEFT_410_SELF_END=2.00
PRIMER_RIGHT_410_SELF_END=2.00
PRIMER_LEFT_410_END_STABILITY=6.9000
PRIMER_RIGHT_410_END_STABILITY=9.1000
PRIMER_PAIR_410_COMPL_ANY=6.00
PRIMER_PAIR_410_COMPL_END=2.00
PRIMER_PAIR_410_PRODUCT_SIZE=75
PRIMER_PAIR_410_PRODUCT_TM=71.6
PRIMER_PAIR_411_PENALTY=6.538057
PRIMER_LEFT_411_PENALTY=3.690586
PRIMER_RIGHT_411_PENALTY=2.847471
PRIMER_LEFT_411_SEQUENCE=CACCAATGTGGTAGAAGCCAAC
PRIMER_RIGHT_411_SEQUENCE=CACAAAGTGGGGATGGGT
PRIMER_LEFT_411=445,22
PRIMER_RIGHT_411=535,18
PRIMER_LEFT_411_TM=61.691
PRIMER_RIGHT_411_TM=59.153
PRIMER_LEFT_411_GC_PERCENT=50.000
PRIMER_RIGHT_411_GC_PERCENT=55.556
PRIMER_LEFT_411_SELF_ANY=6.00
PRIMER_RIGHT_411_SELF_ANY=3.00
PRIMER_LEFT_411_SELF_END=0.00
PRIMER_RIGHT_411_SELF_END=0.00
PRIMER_LEFT_411_END_STABILITY=8.2000
PRIMER_RIGHT_411_END_STABILITY=9.4000
PRIMER_PAIR_411_COMPL_ANY=4.00
PRIMER_PAIR_411_COMPL_END=3.00
PRIMER_PAIR_411_PRODUCT_SIZE=91
PRIMER_PAIR_411_PRODUCT_TM=76.7
PRIMER_PAIR_412_PENALTY=6.559830
PRIMER_LEFT_412_PENALTY=3.756700
PRIMER_RIGHT_412_PENALTY=2.803130
PRIMER_LEFT_412_SEQUENCE=ACCAACCAGTGACCATCCAGA
PRIMER_RIGHT_412_SEQUENCE=GCAGCGGTAGGGAATCAC
PRIMER_LEFT_412=465,21
PRIMER_RIGHT_412=550,18
PRIMER_LEFT_412_TM=62.757
PRIMER_RIGHT_412_TM=59.197
PRIMER_LEFT_412_GC_PERCENT=52.381
PRIMER_RIGHT_412_GC_PERCENT=61.111
PRIMER_LEFT_412_SELF_ANY=3.00
PRIMER_RIGHT_412_SELF_ANY=3.00
PRIMER_LEFT_412_SELF_END=2.00
PRIMER_RIGHT_412_SELF_END=3.00
PRIMER_LEFT_412_END_STABILITY=8.2000
PRIMER_RIGHT_412_END_STABILITY=6.3000
PRIMER_PAIR_412_COMPL_ANY=4.00
PRIMER_PAIR_412_COMPL_END=1.00
PRIMER_PAIR_412_PRODUCT_SIZE=86
PRIMER_PAIR_412_PRODUCT_TM=77.2
PRIMER_PAIR_413_PENALTY=6.564387
PRIMER_LEFT_413_PENALTY=1.757583
PRIMER_RIGHT_413_PENALTY=4.806804
PRIMER_LEFT_413_SEQUENCE=ATGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_413_SEQUENCE=TCTGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_413=345,20
PRIMER_RIGHT_413=443,24
PRIMER_LEFT_413_TM=58.242
PRIMER_RIGHT_413_TM=60.807
PRIMER_LEFT_413_GC_PERCENT=45.000
PRIMER_RIGHT_413_GC_PERCENT=45.833
PRIMER_LEFT_413_SELF_ANY=3.00
PRIMER_RIGHT_413_SELF_ANY=6.00
PRIMER_LEFT_413_SELF_END=2.00
PRIMER_RIGHT_413_SELF_END=2.00
PRIMER_LEFT_413_END_STABILITY=6.6000
PRIMER_RIGHT_413_END_STABILITY=7.0000
PRIMER_PAIR_413_COMPL_ANY=5.00
PRIMER_PAIR_413_COMPL_END=1.00
PRIMER_PAIR_413_PRODUCT_SIZE=99
PRIMER_PAIR_413_PRODUCT_TM=73.7
PRIMER_PAIR_414_PENALTY=6.566269
PRIMER_LEFT_414_PENALTY=3.205809
PRIMER_RIGHT_414_PENALTY=3.360461
PRIMER_LEFT_414_SEQUENCE=TACCGCTGCTTAGTTGGTGAGT
PRIMER_RIGHT_414_SEQUENCE=TCCTCTCCTGGTGTAAGAATTTG
PRIMER_LEFT_414=542,22
PRIMER_RIGHT_414=620,23
PRIMER_LEFT_414_TM=61.206
PRIMER_RIGHT_414_TM=59.640
PRIMER_LEFT_414_GC_PERCENT=50.000
PRIMER_RIGHT_414_GC_PERCENT=43.478
PRIMER_LEFT_414_SELF_ANY=3.00
PRIMER_RIGHT_414_SELF_ANY=4.00
PRIMER_LEFT_414_SELF_END=1.00
PRIMER_RIGHT_414_SELF_END=1.00
PRIMER_LEFT_414_END_STABILITY=6.4000
PRIMER_RIGHT_414_END_STABILITY=7.2000
PRIMER_PAIR_414_COMPL_ANY=4.00
PRIMER_PAIR_414_COMPL_END=0.00
PRIMER_PAIR_414_PRODUCT_SIZE=79
PRIMER_PAIR_414_PRODUCT_TM=71.5
PRIMER_PAIR_415_PENALTY=6.567389
PRIMER_LEFT_415_PENALTY=3.612510
PRIMER_RIGHT_415_PENALTY=2.954879
PRIMER_LEFT_415_SEQUENCE=CCAGAACTGGTGCAAGCG
PRIMER_RIGHT_415_SEQUENCE=AACTAAGCAGCGGTAGGGAATC
PRIMER_LEFT_415=481,18
PRIMER_RIGHT_415=556,22
PRIMER_LEFT_415_TM=61.613
PRIMER_RIGHT_415_TM=60.955
PRIMER_LEFT_415_GC_PERCENT=61.111
PRIMER_RIGHT_415_GC_PERCENT=50.000
PRIMER_LEFT_415_SELF_ANY=6.00
PRIMER_RIGHT_415_SELF_ANY=3.00
PRIMER_LEFT_415_SELF_END=2.00
PRIMER_RIGHT_415_SELF_END=3.00
PRIMER_LEFT_415_END_STABILITY=10.2000
PRIMER_RIGHT_415_END_STABILITY=6.6000
PRIMER_PAIR_415_COMPL_ANY=4.00
PRIMER_PAIR_415_COMPL_END=1.00
PRIMER_PAIR_415_PRODUCT_SIZE=76
PRIMER_PAIR_415_PRODUCT_TM=75.7
PRIMER_PAIR_416_PENALTY=6.571430
PRIMER_LEFT_416_PENALTY=2.660713
PRIMER_RIGHT_416_PENALTY=3.910717
PRIMER_LEFT_416_SEQUENCE=TGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_416_SEQUENCE=GCACCAGTTCTGGATGGTCAC
PRIMER_LEFT_416=416,22
PRIMER_RIGHT_416=493,21
PRIMER_LEFT_416_TM=60.661
PRIMER_RIGHT_416_TM=62.911
PRIMER_LEFT_416_GC_PERCENT=50.000
PRIMER_RIGHT_416_GC_PERCENT=57.143
PRIMER_LEFT_416_SELF_ANY=4.00
PRIMER_RIGHT_416_SELF_ANY=6.00
PRIMER_LEFT_416_SELF_END=0.00
PRIMER_RIGHT_416_SELF_END=3.00
PRIMER_LEFT_416_END_STABILITY=6.7000
PRIMER_RIGHT_416_END_STABILITY=6.1000
PRIMER_PAIR_416_COMPL_ANY=5.00
PRIMER_PAIR_416_COMPL_END=3.00
PRIMER_PAIR_416_PRODUCT_SIZE=78
PRIMER_PAIR_416_PRODUCT_TM=73.2
PRIMER_PAIR_417_PENALTY=6.583729
PRIMER_LEFT_417_PENALTY=4.004895
PRIMER_RIGHT_417_PENALTY=2.578834
PRIMER_LEFT_417_SEQUENCE=AGGTACCCACTGATGGTAATGCT
PRIMER_RIGHT_417_SEQUENCE=CACTTCCCATTCTGGACATT
PRIMER_LEFT_417=255,23
PRIMER_RIGHT_417=354,20
PRIMER_LEFT_417_TM=61.005
PRIMER_RIGHT_417_TM=57.421
PRIMER_LEFT_417_GC_PERCENT=47.826
PRIMER_RIGHT_417_GC_PERCENT=45.000
PRIMER_LEFT_417_SELF_ANY=6.00
PRIMER_RIGHT_417_SELF_ANY=3.00
PRIMER_LEFT_417_SELF_END=0.00
PRIMER_RIGHT_417_SELF_END=1.00
PRIMER_LEFT_417_END_STABILITY=8.1000
PRIMER_RIGHT_417_END_STABILITY=6.6000
PRIMER_PAIR_417_COMPL_ANY=5.00
PRIMER_PAIR_417_COMPL_END=3.00
PRIMER_PAIR_417_PRODUCT_SIZE=100
PRIMER_PAIR_417_PRODUCT_TM=75.2
PRIMER_PAIR_418_PENALTY=6.629471
PRIMER_LEFT_418_PENALTY=3.788644
PRIMER_RIGHT_418_PENALTY=2.840827
PRIMER_LEFT_418_SEQUENCE=CAACCAACCAGTGACCATC
PRIMER_RIGHT_418_SEQUENCE=GCGGTAGGGAATCACAAAG
PRIMER_LEFT_418=463,19
PRIMER_RIGHT_418=547,19
PRIMER_LEFT_418_TM=57.211
PRIMER_RIGHT_418_TM=58.159
PRIMER_LEFT_418_GC_PERCENT=52.632
PRIMER_RIGHT_418_GC_PERCENT=52.632
PRIMER_LEFT_418_SELF_ANY=3.00
PRIMER_RIGHT_418_SELF_ANY=3.00
PRIMER_LEFT_418_SELF_END=1.00
PRIMER_RIGHT_418_SELF_END=0.00
PRIMER_LEFT_418_END_STABILITY=8.1000
PRIMER_RIGHT_418_END_STABILITY=7.3000
PRIMER_PAIR_418_COMPL_ANY=4.00
PRIMER_PAIR_418_COMPL_END=1.00
PRIMER_PAIR_418_PRODUCT_SIZE=85
PRIMER_PAIR_418_PRODUCT_TM=77.0
PRIMER_PAIR_419_PENALTY=6.645541
PRIMER_LEFT_419_PENALTY=2.419726
PRIMER_RIGHT_419_PENALTY=4.225815
PRIMER_LEFT_419_SEQUENCE=TTTGCGAAACTCATCTTCACTG
PRIMER_RIGHT_419_SEQUENCE=CGTAGTCATGCAAGTTGGTACTCT
PRIMER_LEFT_419=627,22
PRIMER_RIGHT_419=704,24
PRIMER_LEFT_419_TM=60.420
PRIMER_RIGHT_419_TM=59.774
PRIMER_LEFT_419_GC_PERCENT=40.909
PRIMER_RIGHT_419_GC_PERCENT=45.833
PRIMER_LEFT_419_SELF_ANY=4.00
PRIMER_RIGHT_419_SELF_ANY=5.00
PRIMER_LEFT_419_SELF_END=3.00
PRIMER_RIGHT_419_SELF_END=0.00
PRIMER_LEFT_419_END_STABILITY=6.7000
PRIMER_RIGHT_419_END_STABILITY=6.1000
PRIMER_PAIR_419_COMPL_ANY=4.00
PRIMER_PAIR_419_COMPL_END=2.00
PRIMER_PAIR_419_PRODUCT_SIZE=78
PRIMER_PAIR_419_PRODUCT_TM=72.2
PRIMER_PAIR_420_PENALTY=6.664614
PRIMER_LEFT_420_PENALTY=4.457412
PRIMER_RIGHT_420_PENALTY=2.207202
PRIMER_LEFT_420_SEQUENCE=TGATGGTAATGCTGGCCT
PRIMER_RIGHT_420_SEQUENCE=TCTGAATCCCACTTCCCATTC
PRIMER_LEFT_420=265,18
PRIMER_RIGHT_420=363,21
PRIMER_LEFT_420_TM=57.543
PRIMER_RIGHT_420_TM=61.207
PRIMER_LEFT_420_GC_PERCENT=50.000
PRIMER_RIGHT_420_GC_PERCENT=47.619
PRIMER_LEFT_420_SELF_ANY=4.00
PRIMER_RIGHT_420_SELF_ANY=4.00
PRIMER_LEFT_420_SELF_END=2.00
PRIMER_RIGHT_420_SELF_END=2.00
PRIMER_LEFT_420_END_STABILITY=10.9000
PRIMER_RIGHT_420_END_STABILITY=6.9000
PRIMER_PAIR_420_COMPL_ANY=5.00
PRIMER_PAIR_420_COMPL_END=3.00
PRIMER_PAIR_420_PRODUCT_SIZE=99
PRIMER_PAIR_420_PRODUCT_TM=74.5
PRIMER_PAIR_421_PENALTY=6.696336
PRIMER_LEFT_421_PENALTY=2.085443
PRIMER_RIGHT_421_PENALTY=4.610893
PRIMER_LEFT_421_SEQUENCE=CATCAGGGACCAAAACCTG
PRIMER_RIGHT_421_SEQUENCE=GGCTTCTACCACATTGGTGATCT
PRIMER_LEFT_421=366,19
PRIMER_RIGHT_421=463,23
PRIMER_LEFT_421_TM=58.915
PRIMER_RIGHT_421_TM=61.611
PRIMER_LEFT_421_GC_PERCENT=52.632
PRIMER_RIGHT_421_GC_PERCENT=47.826
PRIMER_LEFT_421_SELF_ANY=4.00
PRIMER_RIGHT_421_SELF_ANY=6.00
PRIMER_LEFT_421_SELF_END=1.00
PRIMER_RIGHT_421_SELF_END=2.00
PRIMER_LEFT_421_END_STABILITY=7.9000
PRIMER_RIGHT_421_END_STABILITY=6.3000
PRIMER_PAIR_421_COMPL_ANY=5.00
PRIMER_PAIR_421_COMPL_END=1.00
PRIMER_PAIR_421_PRODUCT_SIZE=98
PRIMER_PAIR_421_PRODUCT_TM=73.9
PRIMER_PAIR_422_PENALTY=6.701185
PRIMER_LEFT_422_PENALTY=3.799463
PRIMER_RIGHT_422_PENALTY=2.901722
PRIMER_LEFT_422_SEQUENCE=AGATCACCAATGTGGTAGAAGC
PRIMER_RIGHT_422_SEQUENCE=GGGGATGGGTCTTGCACT
PRIMER_LEFT_422=441,22
PRIMER_RIGHT_422=527,18
PRIMER_LEFT_422_TM=58.201
PRIMER_RIGHT_422_TM=60.902
PRIMER_LEFT_422_GC_PERCENT=45.455
PRIMER_RIGHT_422_GC_PERCENT=61.111
PRIMER_LEFT_422_SELF_ANY=6.00
PRIMER_RIGHT_422_SELF_ANY=4.00
PRIMER_LEFT_422_SELF_END=2.00
PRIMER_RIGHT_422_SELF_END=1.00
PRIMER_LEFT_422_END_STABILITY=8.2000
PRIMER_RIGHT_422_END_STABILITY=7.9000
PRIMER_PAIR_422_COMPL_ANY=4.00
PRIMER_PAIR_422_COMPL_END=2.00
PRIMER_PAIR_422_PRODUCT_SIZE=87
PRIMER_PAIR_422_PRODUCT_TM=76.6
PRIMER_PAIR_423_PENALTY=6.703381
PRIMER_LEFT_423_PENALTY=5.382554
PRIMER_RIGHT_423_PENALTY=1.320826
PRIMER_LEFT_423_SEQUENCE=AGACATGCAGTGAGAAGAGTACC
PRIMER_RIGHT_423_SEQUENCE=AGTGGGCAACACACAAACTCT
PRIMER_LEFT_423=666,23
PRIMER_RIGHT_423=765,21
PRIMER_LEFT_423_TM=57.617
PRIMER_RIGHT_423_TM=59.679
PRIMER_LEFT_423_GC_PERCENT=47.826
PRIMER_RIGHT_423_GC_PERCENT=47.619
PRIMER_LEFT_423_SELF_ANY=4.00
PRIMER_RIGHT_423_SELF_ANY=3.00
PRIMER_LEFT_423_SELF_END=2.00
PRIMER_RIGHT_423_SELF_END=1.00
PRIMER_LEFT_423_END_STABILITY=6.6000
PRIMER_RIGHT_423_END_STABILITY=6.1000
PRIMER_PAIR_423_COMPL_ANY=5.00
PRIMER_PAIR_423_COMPL_END=2.00
PRIMER_PAIR_423_PRODUCT_SIZE=100
PRIMER_PAIR_423_PRODUCT_TM=74.8
PRIMER_PAIR_424_PENALTY=6.718133
PRIMER_LEFT_424_PENALTY=3.207117
PRIMER_RIGHT_424_PENALTY=3.511015
PRIMER_LEFT_424_SEQUENCE=AGTGATGCCCTTCTCGTTC
PRIMER_RIGHT_424_SEQUENCE=GGTGTGCCAGTGAAGATGAGTT
PRIMER_LEFT_424=569,19
PRIMER_RIGHT_424=655,22
PRIMER_LEFT_424_TM=57.793
PRIMER_RIGHT_424_TM=61.511
PRIMER_LEFT_424_GC_PERCENT=52.632
PRIMER_RIGHT_424_GC_PERCENT=50.000
PRIMER_LEFT_424_SELF_ANY=2.00
PRIMER_RIGHT_424_SELF_ANY=3.00
PRIMER_LEFT_424_SELF_END=0.00
PRIMER_RIGHT_424_SELF_END=0.00
PRIMER_LEFT_424_END_STABILITY=8.4000
PRIMER_RIGHT_424_END_STABILITY=6.4000
PRIMER_PAIR_424_COMPL_ANY=4.00
PRIMER_PAIR_424_COMPL_END=1.00
PRIMER_PAIR_424_PRODUCT_SIZE=87
PRIMER_PAIR_424_PRODUCT_TM=72.8
PRIMER_PAIR_425_PENALTY=6.723436
PRIMER_LEFT_425_PENALTY=3.293963
PRIMER_RIGHT_425_PENALTY=3.429473
PRIMER_LEFT_425_SEQUENCE=GCTGCTTAGTTGGTGAGTTTG
PRIMER_RIGHT_425_SEQUENCE=TTCGCAAACATCCATCCTCTC
PRIMER_LEFT_425=546,21
PRIMER_RIGHT_425=634,21
PRIMER_LEFT_425_TM=57.706
PRIMER_RIGHT_425_TM=62.429
PRIMER_LEFT_425_GC_PERCENT=47.619
PRIMER_RIGHT_425_GC_PERCENT=47.619
PRIMER_LEFT_425_SELF_ANY=3.00
PRIMER_RIGHT_425_SELF_ANY=4.00
PRIMER_LEFT_425_SELF_END=0.00
PRIMER_RIGHT_425_SELF_END=0.00
PRIMER_LEFT_425_END_STABILITY=7.0000
PRIMER_RIGHT_425_END_STABILITY=6.4000
PRIMER_PAIR_425_COMPL_ANY=5.00
PRIMER_PAIR_425_COMPL_END=3.00
PRIMER_PAIR_425_PRODUCT_SIZE=89
PRIMER_PAIR_425_PRODUCT_TM=72.0
PRIMER_PAIR_426_PENALTY=6.752968
PRIMER_LEFT_426_PENALTY=4.823759
PRIMER_RIGHT_426_PENALTY=1.929209
PRIMER_LEFT_426_SEQUENCE=GCCAAAGAGACATGCAGTGAGA
PRIMER_RIGHT_426_SEQUENCE=TACCCCTCGGAACTTGTCA
PRIMER_LEFT_426=659,22
PRIMER_RIGHT_426=745,19
PRIMER_LEFT_426_TM=62.824
PRIMER_RIGHT_426_TM=59.071
PRIMER_LEFT_426_GC_PERCENT=50.000
PRIMER_RIGHT_426_GC_PERCENT=52.632
PRIMER_LEFT_426_SELF_ANY=4.00
PRIMER_RIGHT_426_SELF_ANY=3.00
PRIMER_LEFT_426_SELF_END=0.00
PRIMER_RIGHT_426_SELF_END=3.00
PRIMER_LEFT_426_END_STABILITY=6.7000
PRIMER_RIGHT_426_END_STABILITY=6.7000
PRIMER_PAIR_426_COMPL_ANY=4.00
PRIMER_PAIR_426_COMPL_END=3.00
PRIMER_PAIR_426_PRODUCT_SIZE=87
PRIMER_PAIR_426_PRODUCT_TM=74.2
PRIMER_PAIR_427_PENALTY=6.764893
PRIMER_LEFT_427_PENALTY=2.460554
PRIMER_RIGHT_427_PENALTY=4.304339
PRIMER_LEFT_427_SEQUENCE=TGCGAAACTCATCTTCACTG
PRIMER_RIGHT_427_SEQUENCE=GCCGTAGTCATGCAAGTTGGTA
PRIMER_LEFT_427=629,20
PRIMER_RIGHT_427=706,22
PRIMER_LEFT_427_TM=57.539
PRIMER_RIGHT_427_TM=62.304
PRIMER_LEFT_427_GC_PERCENT=45.000
PRIMER_RIGHT_427_GC_PERCENT=50.000
PRIMER_LEFT_427_SELF_ANY=3.00
PRIMER_RIGHT_427_SELF_ANY=5.00
PRIMER_LEFT_427_SELF_END=3.00
PRIMER_RIGHT_427_SELF_END=2.00
PRIMER_LEFT_427_END_STABILITY=6.7000
PRIMER_RIGHT_427_END_STABILITY=7.2000
PRIMER_PAIR_427_COMPL_ANY=4.00
PRIMER_PAIR_427_COMPL_END=3.00
PRIMER_PAIR_427_PRODUCT_SIZE=78
PRIMER_PAIR_427_PRODUCT_TM=73.2
PRIMER_PAIR_428_PENALTY=6.779108
PRIMER_LEFT_428_PENALTY=2.538752
PRIMER_RIGHT_428_PENALTY=4.240356
PRIMER_LEFT_428_SEQUENCE=CTTACACCAGGAGAGGATGGAT
PRIMER_RIGHT_428_SEQUENCE=CTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_428=604,22
PRIMER_RIGHT_428=682,22
PRIMER_LEFT_428_TM=59.461
PRIMER_RIGHT_428_TM=57.760
PRIMER_LEFT_428_GC_PERCENT=50.000
PRIMER_RIGHT_428_GC_PERCENT=45.455
PRIMER_LEFT_428_SELF_ANY=3.00
PRIMER_RIGHT_428_SELF_ANY=4.00
PRIMER_LEFT_428_SELF_END=2.00
PRIMER_RIGHT_428_SELF_END=0.00
PRIMER_LEFT_428_END_STABILITY=8.1000
PRIMER_RIGHT_428_END_STABILITY=7.3000
PRIMER_PAIR_428_COMPL_ANY=5.00
PRIMER_PAIR_428_COMPL_END=3.00
PRIMER_PAIR_428_PRODUCT_SIZE=79
PRIMER_PAIR_428_PRODUCT_TM=73.1
PRIMER_PAIR_429_PENALTY=6.780052
PRIMER_LEFT_429_PENALTY=3.878330
PRIMER_RIGHT_429_PENALTY=2.901722
PRIMER_LEFT_429_SEQUENCE=GATCACCAATGTGGTAGAAGC
PRIMER_RIGHT_429_SEQUENCE=AGTGGGGATGGGTCTTGC
PRIMER_LEFT_429=442,21
PRIMER_RIGHT_429=530,18
PRIMER_LEFT_429_TM=57.122
PRIMER_RIGHT_429_TM=60.902
PRIMER_LEFT_429_GC_PERCENT=47.619
PRIMER_RIGHT_429_GC_PERCENT=61.111
PRIMER_LEFT_429_SELF_ANY=6.00
PRIMER_RIGHT_429_SELF_ANY=2.00
PRIMER_LEFT_429_SELF_END=2.00
PRIMER_RIGHT_429_SELF_END=2.00
PRIMER_LEFT_429_END_STABILITY=8.2000
PRIMER_RIGHT_429_END_STABILITY=8.5000
PRIMER_PAIR_429_COMPL_ANY=5.00
PRIMER_PAIR_429_COMPL_END=2.00
PRIMER_PAIR_429_PRODUCT_SIZE=89
PRIMER_PAIR_429_PRODUCT_TM=76.7
PRIMER_PAIR_430_PENALTY=6.817747
PRIMER_LEFT_430_PENALTY=3.107370
PRIMER_RIGHT_430_PENALTY=3.710377
PRIMER_LEFT_430_SEQUENCE=TGCAAATTCTTACACCAGGAG
PRIMER_RIGHT_430_SEQUENCE=ACTGCATGTCTCTTTGGCGAC
PRIMER_LEFT_430=596,21
PRIMER_RIGHT_430=676,21
PRIMER_LEFT_430_TM=57.893
PRIMER_RIGHT_430_TM=62.710
PRIMER_LEFT_430_GC_PERCENT=42.857
PRIMER_RIGHT_430_GC_PERCENT=52.381
PRIMER_LEFT_430_SELF_ANY=4.00
PRIMER_RIGHT_430_SELF_ANY=4.00
PRIMER_LEFT_430_SELF_END=3.00
PRIMER_RIGHT_430_SELF_END=2.00
PRIMER_LEFT_430_END_STABILITY=7.9000
PRIMER_RIGHT_430_END_STABILITY=9.6000
PRIMER_PAIR_430_COMPL_ANY=4.00
PRIMER_PAIR_430_COMPL_END=1.00
PRIMER_PAIR_430_PRODUCT_SIZE=81
PRIMER_PAIR_430_PRODUCT_TM=72.2
PRIMER_PAIR_431_PENALTY=6.842086
PRIMER_LEFT_431_PENALTY=3.309654
PRIMER_RIGHT_431_PENALTY=3.532432
PRIMER_LEFT_431_SEQUENCE=CCCCACTTTGTGATTCCC
PRIMER_RIGHT_431_SEQUENCE=AGAATTTGCACTTGTCAGGAA
PRIMER_LEFT_431=524,18
PRIMER_RIGHT_431=605,21
PRIMER_LEFT_431_TM=58.690
PRIMER_RIGHT_431_TM=57.468
PRIMER_LEFT_431_GC_PERCENT=55.556
PRIMER_RIGHT_431_GC_PERCENT=38.095
PRIMER_LEFT_431_SELF_ANY=3.00
PRIMER_RIGHT_431_SELF_ANY=4.00
PRIMER_LEFT_431_SELF_END=3.00
PRIMER_RIGHT_431_SELF_END=0.00
PRIMER_LEFT_431_END_STABILITY=9.7000
PRIMER_RIGHT_431_END_STABILITY=8.2000
PRIMER_PAIR_431_COMPL_ANY=4.00
PRIMER_PAIR_431_COMPL_END=3.00
PRIMER_PAIR_431_PRODUCT_SIZE=82
PRIMER_PAIR_431_PRODUCT_TM=72.1
PRIMER_PAIR_432_PENALTY=6.851355
PRIMER_LEFT_432_PENALTY=2.491155
PRIMER_RIGHT_432_PENALTY=4.360200
PRIMER_LEFT_432_SEQUENCE=GGATGTTTGCGAAACTCATCTT
PRIMER_RIGHT_432_SEQUENCE=CATGCCGTAGTCATGCAA
PRIMER_LEFT_432=622,22
PRIMER_RIGHT_432=709,18
PRIMER_LEFT_432_TM=60.491
PRIMER_RIGHT_432_TM=57.640
PRIMER_LEFT_432_GC_PERCENT=40.909
PRIMER_RIGHT_432_GC_PERCENT=50.000
PRIMER_LEFT_432_SELF_ANY=6.00
PRIMER_RIGHT_432_SELF_ANY=6.00
PRIMER_LEFT_432_SELF_END=2.00
PRIMER_RIGHT_432_SELF_END=2.00
PRIMER_LEFT_432_END_STABILITY=6.6000
PRIMER_RIGHT_432_END_STABILITY=8.8000
PRIMER_PAIR_432_COMPL_ANY=4.00
PRIMER_PAIR_432_COMPL_END=2.00
PRIMER_PAIR_432_PRODUCT_SIZE=88
PRIMER_PAIR_432_PRODUCT_TM=73.6
PRIMER_PAIR_433_PENALTY=6.854565
PRIMER_LEFT_433_PENALTY=5.365271
PRIMER_RIGHT_433_PENALTY=1.489294
PRIMER_LEFT_433_SEQUENCE=AGAGACATGCAGTGAGAAGAGTACC
PRIMER_RIGHT_433_SEQUENCE=GGCAACACACAAACTCTACCC
PRIMER_LEFT_433=664,25
PRIMER_RIGHT_433=761,21
PRIMER_LEFT_433_TM=60.365
PRIMER_RIGHT_433_TM=59.511
PRIMER_LEFT_433_GC_PERCENT=48.000
PRIMER_RIGHT_433_GC_PERCENT=52.381
PRIMER_LEFT_433_SELF_ANY=4.00
PRIMER_RIGHT_433_SELF_ANY=2.00
PRIMER_LEFT_433_SELF_END=2.00
PRIMER_RIGHT_433_SELF_END=0.00
PRIMER_LEFT_433_END_STABILITY=6.6000
PRIMER_RIGHT_433_END_STABILITY=8.4000
PRIMER_PAIR_433_COMPL_ANY=5.00
PRIMER_PAIR_433_COMPL_END=2.00
PRIMER_PAIR_433_PRODUCT_SIZE=98
PRIMER_PAIR_433_PRODUCT_TM=74.7
PRIMER_PAIR_434_PENALTY=6.873083
PRIMER_LEFT_434_PENALTY=3.791133
PRIMER_RIGHT_434_PENALTY=3.081950
PRIMER_LEFT_434_SEQUENCE=CAGTATTGCCAAGAAGTCTACCC
PRIMER_RIGHT_434_SEQUENCE=TCTGGATGGTCACTGGTTG
PRIMER_LEFT_434=410,23
PRIMER_RIGHT_434=485,19
PRIMER_LEFT_434_TM=59.209
PRIMER_RIGHT_434_TM=57.918
PRIMER_LEFT_434_GC_PERCENT=47.826
PRIMER_RIGHT_434_GC_PERCENT=52.632
PRIMER_LEFT_434_SELF_ANY=5.00
PRIMER_RIGHT_434_SELF_ANY=3.00
PRIMER_LEFT_434_SELF_END=0.00
PRIMER_RIGHT_434_SELF_END=0.00
PRIMER_LEFT_434_END_STABILITY=8.4000
PRIMER_RIGHT_434_END_STABILITY=8.2000
PRIMER_PAIR_434_COMPL_ANY=4.00
PRIMER_PAIR_434_COMPL_END=3.00
PRIMER_PAIR_434_PRODUCT_SIZE=76
PRIMER_PAIR_434_PRODUCT_TM=72.0
PRIMER_PAIR_435_PENALTY=6.881481
PRIMER_LEFT_435_PENALTY=2.540392
PRIMER_RIGHT_435_PENALTY=4.341090
PRIMER_LEFT_435_SEQUENCE=TTACACCAGGAGAGGATGGAT
PRIMER_RIGHT_435_SEQUENCE=CTTCTCACTGCATGTCTCTTTGG
PRIMER_LEFT_435=605,21
PRIMER_RIGHT_435=682,23
PRIMER_LEFT_435_TM=58.460
PRIMER_RIGHT_435_TM=61.341
PRIMER_LEFT_435_GC_PERCENT=47.619
PRIMER_RIGHT_435_GC_PERCENT=47.826
PRIMER_LEFT_435_SELF_ANY=3.00
PRIMER_RIGHT_435_SELF_ANY=4.00
PRIMER_LEFT_435_SELF_END=2.00
PRIMER_RIGHT_435_SELF_END=0.00
PRIMER_LEFT_435_END_STABILITY=8.1000
PRIMER_RIGHT_435_END_STABILITY=8.8000
PRIMER_PAIR_435_COMPL_ANY=5.00
PRIMER_PAIR_435_COMPL_END=3.00
PRIMER_PAIR_435_PRODUCT_SIZE=78
PRIMER_PAIR_435_PRODUCT_TM=72.7
PRIMER_PAIR_436_PENALTY=6.886599
PRIMER_LEFT_436_PENALTY=3.253441
PRIMER_RIGHT_436_PENALTY=3.633158
PRIMER_LEFT_436_SEQUENCE=ATGCCCTTCTCGTTCCTG
PRIMER_RIGHT_436_SEQUENCE=GTGCCAGTGAAGATGAGTTTC
PRIMER_LEFT_436=573,18
PRIMER_RIGHT_436=652,21
PRIMER_LEFT_436_TM=58.747
PRIMER_RIGHT_436_TM=57.367
PRIMER_LEFT_436_GC_PERCENT=55.556
PRIMER_RIGHT_436_GC_PERCENT=47.619
PRIMER_LEFT_436_SELF_ANY=2.00
PRIMER_RIGHT_436_SELF_ANY=3.00
PRIMER_LEFT_436_SELF_END=1.00
PRIMER_RIGHT_436_SELF_END=3.00
PRIMER_LEFT_436_END_STABILITY=8.2000
PRIMER_RIGHT_436_END_STABILITY=6.7000
PRIMER_PAIR_436_COMPL_ANY=4.00
PRIMER_PAIR_436_COMPL_END=1.00
PRIMER_PAIR_436_PRODUCT_SIZE=80
PRIMER_PAIR_436_PRODUCT_TM=71.9
PRIMER_PAIR_437_PENALTY=6.898372
PRIMER_LEFT_437_PENALTY=3.117810
PRIMER_RIGHT_437_PENALTY=3.780562
PRIMER_LEFT_437_SEQUENCE=TTCCTGACAAGTGCAAATTCTT
PRIMER_RIGHT_437_SEQUENCE=CATGTCTCTTTGGCGACGG
PRIMER_LEFT_437=585,22
PRIMER_RIGHT_437=672,19
PRIMER_LEFT_437_TM=58.882
PRIMER_RIGHT_437_TM=62.781
PRIMER_LEFT_437_GC_PERCENT=36.364
PRIMER_RIGHT_437_GC_PERCENT=57.895
PRIMER_LEFT_437_SELF_ANY=4.00
PRIMER_RIGHT_437_SELF_ANY=4.00
PRIMER_LEFT_437_SELF_END=1.00
PRIMER_RIGHT_437_SELF_END=1.00
PRIMER_LEFT_437_END_STABILITY=7.0000
PRIMER_RIGHT_437_END_STABILITY=9.6000
PRIMER_PAIR_437_COMPL_ANY=4.00
PRIMER_PAIR_437_COMPL_END=0.00
PRIMER_PAIR_437_PRODUCT_SIZE=88
PRIMER_PAIR_437_PRODUCT_TM=72.7
PRIMER_PAIR_438_PENALTY=6.918417
PRIMER_LEFT_438_PENALTY=3.320745
PRIMER_RIGHT_438_PENALTY=3.597672
PRIMER_LEFT_438_SEQUENCE=CTACCGCTGCTTAGTTGGTGAG
PRIMER_RIGHT_438_SEQUENCE=AACATCCATCCTCTCCTGG
PRIMER_LEFT_438=541,22
PRIMER_RIGHT_438=628,19
PRIMER_LEFT_438_TM=61.321
PRIMER_RIGHT_438_TM=57.402
PRIMER_LEFT_438_GC_PERCENT=54.545
PRIMER_RIGHT_438_GC_PERCENT=52.632
PRIMER_LEFT_438_SELF_ANY=3.00
PRIMER_RIGHT_438_SELF_ANY=3.00
PRIMER_LEFT_438_SELF_END=0.00
PRIMER_RIGHT_438_SELF_END=3.00
PRIMER_LEFT_438_END_STABILITY=6.4000
PRIMER_RIGHT_438_END_STABILITY=9.7000
PRIMER_PAIR_438_COMPL_ANY=4.00
PRIMER_PAIR_438_COMPL_END=1.00
PRIMER_PAIR_438_PRODUCT_SIZE=88
PRIMER_PAIR_438_PRODUCT_TM=72.2
PRIMER_PAIR_439_PENALTY=6.979432
PRIMER_LEFT_439_PENALTY=2.186531
PRIMER_RIGHT_439_PENALTY=4.792901
PRIMER_LEFT_439_SEQUENCE=CAGGGACCAAAACCTGCATT
PRIMER_RIGHT_439_SEQUENCE=CTTCTACCACATTGGTGATCTG
PRIMER_LEFT_439=369,20
PRIMER_RIGHT_439=461,22
PRIMER_LEFT_439_TM=62.187
PRIMER_RIGHT_439_TM=57.207
PRIMER_LEFT_439_GC_PERCENT=50.000
PRIMER_RIGHT_439_GC_PERCENT=45.455
PRIMER_LEFT_439_SELF_ANY=4.00
PRIMER_RIGHT_439_SELF_ANY=6.00
PRIMER_LEFT_439_SELF_END=1.00
PRIMER_RIGHT_439_SELF_END=1.00
PRIMER_LEFT_439_END_STABILITY=8.4000
PRIMER_RIGHT_439_END_STABILITY=6.6000
PRIMER_PAIR_439_COMPL_ANY=5.00
PRIMER_PAIR_439_COMPL_END=0.00
PRIMER_PAIR_439_PRODUCT_SIZE=93
PRIMER_PAIR_439_PRODUCT_TM=73.3
PRIMER_PAIR_440_PENALTY=6.992292
PRIMER_LEFT_440_PENALTY=3.738851
PRIMER_RIGHT_440_PENALTY=3.253441
PRIMER_LEFT_440_SEQUENCE=AGACCCATCCCCACTTTG
PRIMER_RIGHT_440_SEQUENCE=CAGGAACGAGAAGGGCAT
PRIMER_LEFT_440=516,18
PRIMER_RIGHT_440=590,18
PRIMER_LEFT_440_TM=58.261
PRIMER_RIGHT_440_TM=58.747
PRIMER_LEFT_440_GC_PERCENT=55.556
PRIMER_RIGHT_440_GC_PERCENT=55.556
PRIMER_LEFT_440_SELF_ANY=2.00
PRIMER_RIGHT_440_SELF_ANY=2.00
PRIMER_LEFT_440_SELF_END=1.00
PRIMER_RIGHT_440_SELF_END=2.00
PRIMER_LEFT_440_END_STABILITY=7.3000
PRIMER_RIGHT_440_END_STABILITY=9.6000
PRIMER_PAIR_440_COMPL_ANY=4.00
PRIMER_PAIR_440_COMPL_END=2.00
PRIMER_PAIR_440_PRODUCT_SIZE=75
PRIMER_PAIR_440_PRODUCT_TM=72.7
PRIMER_PAIR_441_PENALTY=7.010512
PRIMER_LEFT_441_PENALTY=3.668956
PRIMER_RIGHT_441_PENALTY=3.341555
PRIMER_LEFT_441_SEQUENCE=GGGACCAAAACCTGCATT
PRIMER_RIGHT_441_SEQUENCE=TTGGTTGGCTTCTACCACA
PRIMER_LEFT_441=371,18
PRIMER_RIGHT_441=469,19
PRIMER_LEFT_441_TM=58.331
PRIMER_RIGHT_441_TM=57.658
PRIMER_LEFT_441_GC_PERCENT=50.000
PRIMER_RIGHT_441_GC_PERCENT=47.368
PRIMER_LEFT_441_SELF_ANY=4.00
PRIMER_RIGHT_441_SELF_ANY=4.00
PRIMER_LEFT_441_SELF_END=1.00
PRIMER_RIGHT_441_SELF_END=2.00
PRIMER_LEFT_441_END_STABILITY=8.4000
PRIMER_RIGHT_441_END_STABILITY=8.2000
PRIMER_PAIR_441_COMPL_ANY=5.00
PRIMER_PAIR_441_COMPL_END=1.00
PRIMER_PAIR_441_PRODUCT_SIZE=99
PRIMER_PAIR_441_PRODUCT_TM=73.7
PRIMER_PAIR_442_PENALTY=7.020342
PRIMER_LEFT_442_PENALTY=4.566681
PRIMER_RIGHT_442_PENALTY=2.453661
PRIMER_LEFT_442_SEQUENCE=ACATGCACATGAATGTCCAGAAT
PRIMER_RIGHT_442_SEQUENCE=CCTTGGTATCAATGCAGGTT
PRIMER_LEFT_442=324,23
PRIMER_RIGHT_442=398,20
PRIMER_LEFT_442_TM=61.567
PRIMER_RIGHT_442_TM=57.546
PRIMER_LEFT_442_GC_PERCENT=39.130
PRIMER_RIGHT_442_GC_PERCENT=45.000
PRIMER_LEFT_442_SELF_ANY=8.00
PRIMER_RIGHT_442_SELF_ANY=6.00
PRIMER_LEFT_442_SELF_END=3.00
PRIMER_RIGHT_442_SELF_END=0.00
PRIMER_LEFT_442_END_STABILITY=6.6000
PRIMER_RIGHT_442_END_STABILITY=7.9000
PRIMER_PAIR_442_COMPL_ANY=5.00
PRIMER_PAIR_442_COMPL_END=1.00
PRIMER_PAIR_442_PRODUCT_SIZE=75
PRIMER_PAIR_442_PRODUCT_TM=71.0
PRIMER_PAIR_443_PENALTY=7.029754
PRIMER_LEFT_443_PENALTY=3.681420
PRIMER_RIGHT_443_PENALTY=3.348334
PRIMER_LEFT_443_SEQUENCE=GAGGTACCCACTGATGGTAATG
PRIMER_RIGHT_443_SEQUENCE=CATTCATGTGCATGTTCAGTCT
PRIMER_LEFT_443=254,22
PRIMER_RIGHT_443=338,22
PRIMER_LEFT_443_TM=58.319
PRIMER_RIGHT_443_TM=58.652
PRIMER_LEFT_443_GC_PERCENT=50.000
PRIMER_RIGHT_443_GC_PERCENT=40.909
PRIMER_LEFT_443_SELF_ANY=6.00
PRIMER_RIGHT_443_SELF_ANY=6.00
PRIMER_LEFT_443_SELF_END=0.00
PRIMER_RIGHT_443_SELF_END=3.00
PRIMER_LEFT_443_END_STABILITY=6.2000
PRIMER_RIGHT_443_END_STABILITY=6.1000
PRIMER_PAIR_443_COMPL_ANY=5.00
PRIMER_PAIR_443_COMPL_END=1.00
PRIMER_PAIR_443_PRODUCT_SIZE=85
PRIMER_PAIR_443_PRODUCT_TM=74.5
PRIMER_PAIR_444_PENALTY=7.038162
PRIMER_LEFT_444_PENALTY=1.628165
PRIMER_RIGHT_444_PENALTY=5.409997
PRIMER_LEFT_444_SEQUENCE=TCCAGAATGGGAAGTGGGAT
PRIMER_RIGHT_444_SEQUENCE=AGGGTAGACTTCTTGGCAATACT
PRIMER_LEFT_444=339,20
PRIMER_RIGHT_444=433,23
PRIMER_LEFT_444_TM=61.628
PRIMER_RIGHT_444_TM=57.590
PRIMER_LEFT_444_GC_PERCENT=50.000
PRIMER_RIGHT_444_GC_PERCENT=43.478
PRIMER_LEFT_444_SELF_ANY=3.00
PRIMER_RIGHT_444_SELF_ANY=5.00
PRIMER_LEFT_444_SELF_END=2.00
PRIMER_RIGHT_444_SELF_END=2.00
PRIMER_LEFT_444_END_STABILITY=9.3000
PRIMER_RIGHT_444_END_STABILITY=5.3000
PRIMER_PAIR_444_COMPL_ANY=5.00
PRIMER_PAIR_444_COMPL_END=1.00
PRIMER_PAIR_444_PRODUCT_SIZE=95
PRIMER_PAIR_444_PRODUCT_TM=73.4
PRIMER_PAIR_445_PENALTY=7.046990
PRIMER_LEFT_445_PENALTY=2.151267
PRIMER_RIGHT_445_PENALTY=4.895724
PRIMER_LEFT_445_SEQUENCE=AGTGGGATTCAGATCCATCA
PRIMER_RIGHT_445_SEQUENCE=ATCTGCAGTTCAGGGTAGACTT
PRIMER_LEFT_445=351,20
PRIMER_RIGHT_445=444,22
PRIMER_LEFT_445_TM=57.849
PRIMER_RIGHT_445_TM=57.104
PRIMER_LEFT_445_GC_PERCENT=45.000
PRIMER_RIGHT_445_GC_PERCENT=45.455
PRIMER_LEFT_445_SELF_ANY=5.00
PRIMER_RIGHT_445_SELF_ANY=6.00
PRIMER_LEFT_445_SELF_END=2.00
PRIMER_RIGHT_445_SELF_END=1.00
PRIMER_LEFT_445_END_STABILITY=6.9000
PRIMER_RIGHT_445_END_STABILITY=6.4000
PRIMER_PAIR_445_COMPL_ANY=5.00
PRIMER_PAIR_445_COMPL_END=1.00
PRIMER_PAIR_445_PRODUCT_SIZE=94
PRIMER_PAIR_445_PRODUCT_TM=73.1
PRIMER_PAIR_446_PENALTY=7.094532
PRIMER_LEFT_446_PENALTY=4.266053
PRIMER_RIGHT_446_PENALTY=2.828479
PRIMER_LEFT_446_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTA
PRIMER_RIGHT_446_SEQUENCE=GGATGGTCACTGGTTGGTT
PRIMER_LEFT_446=407,23
PRIMER_RIGHT_446=482,19
PRIMER_LEFT_446_TM=58.734
PRIMER_RIGHT_446_TM=58.172
PRIMER_LEFT_446_GC_PERCENT=43.478
PRIMER_RIGHT_446_GC_PERCENT=52.632
PRIMER_LEFT_446_SELF_ANY=6.00
PRIMER_RIGHT_446_SELF_ANY=3.00
PRIMER_LEFT_446_SELF_END=2.00
PRIMER_RIGHT_446_SELF_END=0.00
PRIMER_LEFT_446_END_STABILITY=5.4000
PRIMER_RIGHT_446_END_STABILITY=8.2000
PRIMER_PAIR_446_COMPL_ANY=5.00
PRIMER_PAIR_446_COMPL_END=1.00
PRIMER_PAIR_446_PRODUCT_SIZE=76
PRIMER_PAIR_446_PRODUCT_TM=72.5
PRIMER_PAIR_447_PENALTY=7.095669
PRIMER_LEFT_447_PENALTY=3.409264
PRIMER_RIGHT_447_PENALTY=3.686405
PRIMER_LEFT_447_SEQUENCE=TCCCTACCGCTGCTTAGTT
PRIMER_RIGHT_447_SEQUENCE=CCTGGTGTAAGAATTTGCACTT
PRIMER_LEFT_447=538,19
PRIMER_RIGHT_447=614,22
PRIMER_LEFT_447_TM=57.591
PRIMER_RIGHT_447_TM=58.314
PRIMER_LEFT_447_GC_PERCENT=52.632
PRIMER_RIGHT_447_GC_PERCENT=40.909
PRIMER_LEFT_447_SELF_ANY=3.00
PRIMER_RIGHT_447_SELF_ANY=5.00
PRIMER_LEFT_447_SELF_END=0.00
PRIMER_RIGHT_447_SELF_END=2.00
PRIMER_LEFT_447_END_STABILITY=5.7000
PRIMER_RIGHT_447_END_STABILITY=6.7000
PRIMER_PAIR_447_COMPL_ANY=4.00
PRIMER_PAIR_447_COMPL_END=1.00
PRIMER_PAIR_447_PRODUCT_SIZE=77
PRIMER_PAIR_447_PRODUCT_TM=71.8
PRIMER_PAIR_448_PENALTY=7.112405
PRIMER_LEFT_448_PENALTY=2.561455
PRIMER_RIGHT_448_PENALTY=4.550950
PRIMER_LEFT_448_SEQUENCE=TGCACATGAATGTCCAGAAT
PRIMER_RIGHT_448_SEQUENCE=CTTCCTTGGTATCAATGCAGGTT
PRIMER_LEFT_448=327,20
PRIMER_RIGHT_448=401,23
PRIMER_LEFT_448_TM=57.439
PRIMER_RIGHT_448_TM=61.551
PRIMER_LEFT_448_GC_PERCENT=40.000
PRIMER_RIGHT_448_GC_PERCENT=43.478
PRIMER_LEFT_448_SELF_ANY=8.00
PRIMER_RIGHT_448_SELF_ANY=8.00
PRIMER_LEFT_448_SELF_END=2.00
PRIMER_RIGHT_448_SELF_END=0.00
PRIMER_LEFT_448_END_STABILITY=6.6000
PRIMER_RIGHT_448_END_STABILITY=7.9000
PRIMER_PAIR_448_COMPL_ANY=4.00
PRIMER_PAIR_448_COMPL_END=2.00
PRIMER_PAIR_448_PRODUCT_SIZE=75
PRIMER_PAIR_448_PRODUCT_TM=71.0
PRIMER_PAIR_449_PENALTY=7.127440
PRIMER_LEFT_449_PENALTY=3.409264
PRIMER_RIGHT_449_PENALTY=3.718176
PRIMER_LEFT_449_SEQUENCE=TTCCCTACCGCTGCTTAGT
PRIMER_RIGHT_449_SEQUENCE=CCATCCTCTCCTGGTGTAAGAAT
PRIMER_LEFT_449=537,19
PRIMER_RIGHT_449=623,23
PRIMER_LEFT_449_TM=57.591
PRIMER_RIGHT_449_TM=60.718
PRIMER_LEFT_449_GC_PERCENT=52.632
PRIMER_RIGHT_449_GC_PERCENT=47.826
PRIMER_LEFT_449_SELF_ANY=3.00
PRIMER_RIGHT_449_SELF_ANY=3.00
PRIMER_LEFT_449_SELF_END=1.00
PRIMER_RIGHT_449_SELF_END=2.00
PRIMER_LEFT_449_END_STABILITY=5.7000
PRIMER_RIGHT_449_END_STABILITY=6.6000
PRIMER_PAIR_449_COMPL_ANY=4.00
PRIMER_PAIR_449_COMPL_END=1.00
PRIMER_PAIR_449_PRODUCT_SIZE=87
PRIMER_PAIR_449_PRODUCT_TM=72.8
PRIMER_PAIR_450_PENALTY=7.130001
PRIMER_LEFT_450_PENALTY=2.766033
PRIMER_RIGHT_450_PENALTY=4.363968
PRIMER_LEFT_450_SEQUENCE=TACACCAGGAGAGGATGGATGT
PRIMER_RIGHT_450_SEQUENCE=TGGTACTCTTCTCACTGCATGTCT
PRIMER_LEFT_450=606,22
PRIMER_RIGHT_450=689,24
PRIMER_LEFT_450_TM=60.766
PRIMER_RIGHT_450_TM=60.364
PRIMER_LEFT_450_GC_PERCENT=50.000
PRIMER_RIGHT_450_GC_PERCENT=45.833
PRIMER_LEFT_450_SELF_ANY=4.00
PRIMER_RIGHT_450_SELF_ANY=4.00
PRIMER_LEFT_450_SELF_END=0.00
PRIMER_RIGHT_450_SELF_END=0.00
PRIMER_LEFT_450_END_STABILITY=6.3000
PRIMER_RIGHT_450_END_STABILITY=6.4000
PRIMER_PAIR_450_COMPL_ANY=6.00
PRIMER_PAIR_450_COMPL_END=3.00
PRIMER_PAIR_450_PRODUCT_SIZE=84
PRIMER_PAIR_450_PRODUCT_TM=73.3
PRIMER_PAIR_451_PENALTY=7.139908
PRIMER_LEFT_451_PENALTY=3.895774
PRIMER_RIGHT_451_PENALTY=3.244134
PRIMER_LEFT_451_SEQUENCE=CCAATGTGGTAGAAGCCAA
PRIMER_RIGHT_451_SEQUENCE=AAGTGGGGATGGGTCTTGC
PRIMER_LEFT_451=447,19
PRIMER_RIGHT_451=531,19
PRIMER_LEFT_451_TM=57.104
PRIMER_RIGHT_451_TM=62.244
PRIMER_LEFT_451_GC_PERCENT=47.368
PRIMER_RIGHT_451_GC_PERCENT=57.895
PRIMER_LEFT_451_SELF_ANY=4.00
PRIMER_RIGHT_451_SELF_ANY=3.00
PRIMER_LEFT_451_SELF_END=0.00
PRIMER_RIGHT_451_SELF_END=2.00
PRIMER_LEFT_451_END_STABILITY=10.0000
PRIMER_RIGHT_451_END_STABILITY=8.5000
PRIMER_PAIR_451_COMPL_ANY=3.00
PRIMER_PAIR_451_COMPL_END=2.00
PRIMER_PAIR_451_PRODUCT_SIZE=85
PRIMER_PAIR_451_PRODUCT_TM=76.5
PRIMER_PAIR_452_PENALTY=7.154250
PRIMER_LEFT_452_PENALTY=3.791133
PRIMER_RIGHT_452_PENALTY=3.363116
PRIMER_LEFT_452_SEQUENCE=GTATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_452_SEQUENCE=AGTTCTGGATGGTCACTGGTTG
PRIMER_LEFT_452=412,23
PRIMER_RIGHT_452=488,22
PRIMER_LEFT_452_TM=59.209
PRIMER_RIGHT_452_TM=61.363
PRIMER_LEFT_452_GC_PERCENT=47.826
PRIMER_RIGHT_452_GC_PERCENT=50.000
PRIMER_LEFT_452_SELF_ANY=5.00
PRIMER_RIGHT_452_SELF_ANY=3.00
PRIMER_LEFT_452_SELF_END=1.00
PRIMER_RIGHT_452_SELF_END=0.00
PRIMER_LEFT_452_END_STABILITY=9.7000
PRIMER_RIGHT_452_END_STABILITY=8.2000
PRIMER_PAIR_452_COMPL_ANY=4.00
PRIMER_PAIR_452_COMPL_END=3.00
PRIMER_PAIR_452_PRODUCT_SIZE=77
PRIMER_PAIR_452_PRODUCT_TM=71.8
PRIMER_PAIR_453_PENALTY=7.166911
PRIMER_LEFT_453_PENALTY=3.634478
PRIMER_RIGHT_453_PENALTY=3.532432
PRIMER_LEFT_453_SEQUENCE=ACCCATCCCCACTTTGTGATT
PRIMER_RIGHT_453_SEQUENCE=AAGAATTTGCACTTGTCAGGA
PRIMER_LEFT_453=518,21
PRIMER_RIGHT_453=606,21
PRIMER_LEFT_453_TM=62.634
PRIMER_RIGHT_453_TM=57.468
PRIMER_LEFT_453_GC_PERCENT=47.619
PRIMER_RIGHT_453_GC_PERCENT=38.095
PRIMER_LEFT_453_SELF_ANY=4.00
PRIMER_RIGHT_453_SELF_ANY=4.00
PRIMER_LEFT_453_SELF_END=2.00
PRIMER_RIGHT_453_SELF_END=2.00
PRIMER_LEFT_453_END_STABILITY=6.9000
PRIMER_RIGHT_453_END_STABILITY=8.2000
PRIMER_PAIR_453_COMPL_ANY=3.00
PRIMER_PAIR_453_COMPL_END=1.00
PRIMER_PAIR_453_PRODUCT_SIZE=89
PRIMER_PAIR_453_PRODUCT_TM=72.5
PRIMER_PAIR_454_PENALTY=7.167486
PRIMER_LEFT_454_PENALTY=3.738851
PRIMER_RIGHT_454_PENALTY=3.428635
PRIMER_LEFT_454_SEQUENCE=CAAGACCCATCCCCACTT
PRIMER_RIGHT_454_SEQUENCE=AGGAACGAGAAGGGCATCACT
PRIMER_LEFT_454=514,18
PRIMER_RIGHT_454=589,21
PRIMER_LEFT_454_TM=58.261
PRIMER_RIGHT_454_TM=62.429
PRIMER_LEFT_454_GC_PERCENT=55.556
PRIMER_RIGHT_454_GC_PERCENT=52.381
PRIMER_LEFT_454_SELF_ANY=3.00
PRIMER_RIGHT_454_SELF_ANY=2.00
PRIMER_LEFT_454_SELF_END=0.00
PRIMER_RIGHT_454_SELF_END=2.00
PRIMER_LEFT_454_END_STABILITY=6.7000
PRIMER_RIGHT_454_END_STABILITY=6.4000
PRIMER_PAIR_454_COMPL_ANY=4.00
PRIMER_PAIR_454_COMPL_END=0.00
PRIMER_PAIR_454_PRODUCT_SIZE=76
PRIMER_PAIR_454_PRODUCT_TM=72.5
PRIMER_PAIR_455_PENALTY=7.170029
PRIMER_LEFT_455_PENALTY=3.232712
PRIMER_RIGHT_455_PENALTY=3.937317
PRIMER_LEFT_455_SEQUENCE=GTGCAAATTCTTACACCAGGAG
PRIMER_RIGHT_455_SEQUENCE=GCATGTCTCTTTGGCGACG
PRIMER_LEFT_455=595,22
PRIMER_RIGHT_455=673,19
PRIMER_LEFT_455_TM=58.767
PRIMER_RIGHT_455_TM=62.937
PRIMER_LEFT_455_GC_PERCENT=45.455
PRIMER_RIGHT_455_GC_PERCENT=57.895
PRIMER_LEFT_455_SELF_ANY=5.00
PRIMER_RIGHT_455_SELF_ANY=4.00
PRIMER_LEFT_455_SELF_END=3.00
PRIMER_RIGHT_455_SELF_END=3.00
PRIMER_LEFT_455_END_STABILITY=7.9000
PRIMER_RIGHT_455_END_STABILITY=10.1000
PRIMER_PAIR_455_COMPL_ANY=4.00
PRIMER_PAIR_455_COMPL_END=2.00
PRIMER_PAIR_455_PRODUCT_SIZE=79
PRIMER_PAIR_455_PRODUCT_TM=72.5
PRIMER_PAIR_456_PENALTY=7.207248
PRIMER_LEFT_456_PENALTY=4.366422
PRIMER_RIGHT_456_PENALTY=2.840827
PRIMER_LEFT_456_SEQUENCE=ATGTGGTAGAAGCCAACCAACC
PRIMER_RIGHT_456_SEQUENCE=AGCGGTAGGGAATCACAAA
PRIMER_LEFT_456=450,22
PRIMER_RIGHT_456=548,19
PRIMER_LEFT_456_TM=62.366
PRIMER_RIGHT_456_TM=58.159
PRIMER_LEFT_456_GC_PERCENT=50.000
PRIMER_RIGHT_456_GC_PERCENT=47.368
PRIMER_LEFT_456_SELF_ANY=4.00
PRIMER_RIGHT_456_SELF_ANY=3.00
PRIMER_LEFT_456_SELF_END=0.00
PRIMER_RIGHT_456_SELF_END=1.00
PRIMER_LEFT_456_END_STABILITY=8.2000
PRIMER_RIGHT_456_END_STABILITY=7.0000
PRIMER_PAIR_456_COMPL_ANY=4.00
PRIMER_PAIR_456_COMPL_END=0.00
PRIMER_PAIR_456_PRODUCT_SIZE=99
PRIMER_PAIR_456_PRODUCT_TM=77.0
PRIMER_PAIR_457_PENALTY=7.239166
PRIMER_LEFT_457_PENALTY=4.076046
PRIMER_RIGHT_457_PENALTY=3.163121
PRIMER_LEFT_457_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCT
PRIMER_RIGHT_457_SEQUENCE=CTGGATGGTCACTGGTTGGTT
PRIMER_LEFT_457=410,24
PRIMER_RIGHT_457=484,21
PRIMER_LEFT_457_TM=60.076
PRIMER_RIGHT_457_TM=62.163
PRIMER_LEFT_457_GC_PERCENT=45.833
PRIMER_RIGHT_457_GC_PERCENT=52.381
PRIMER_LEFT_457_SELF_ANY=5.00
PRIMER_RIGHT_457_SELF_ANY=3.00
PRIMER_LEFT_457_SELF_END=2.00
PRIMER_RIGHT_457_SELF_END=0.00
PRIMER_LEFT_457_END_STABILITY=9.1000
PRIMER_RIGHT_457_END_STABILITY=8.2000
PRIMER_PAIR_457_COMPL_ANY=5.00
PRIMER_PAIR_457_COMPL_END=3.00
PRIMER_PAIR_457_PRODUCT_SIZE=75
PRIMER_PAIR_457_PRODUCT_TM=72.1
PRIMER_PAIR_458_PENALTY=7.264160
PRIMER_LEFT_458_PENALTY=2.788676
PRIMER_RIGHT_458_PENALTY=4.475484
PRIMER_LEFT_458_SEQUENCE=CCAGGAGAGGATGGATGTTTG
PRIMER_RIGHT_458_SEQUENCE=TCATGCAAGTTGGTACTCTTCTC
PRIMER_LEFT_458=610,21
PRIMER_RIGHT_458=699,23
PRIMER_LEFT_458_TM=61.789
PRIMER_RIGHT_458_TM=58.525
PRIMER_LEFT_458_GC_PERCENT=52.381
PRIMER_RIGHT_458_GC_PERCENT=43.478
PRIMER_LEFT_458_SELF_ANY=3.00
PRIMER_RIGHT_458_SELF_ANY=5.00
PRIMER_LEFT_458_SELF_END=0.00
PRIMER_RIGHT_458_SELF_END=0.00
PRIMER_LEFT_458_END_STABILITY=7.0000
PRIMER_RIGHT_458_END_STABILITY=6.7000
PRIMER_PAIR_458_COMPL_ANY=5.00
PRIMER_PAIR_458_COMPL_END=1.00
PRIMER_PAIR_458_PRODUCT_SIZE=90
PRIMER_PAIR_458_PRODUCT_TM=73.7
PRIMER_PAIR_459_PENALTY=7.278031
PRIMER_LEFT_459_PENALTY=3.835187
PRIMER_RIGHT_459_PENALTY=3.442844
PRIMER_LEFT_459_SEQUENCE=GGAGGTACCCACTGATGGT
PRIMER_RIGHT_459_SEQUENCE=GACATTCATGTGCATGTTCAGTC
PRIMER_LEFT_459=253,19
PRIMER_RIGHT_459=340,23
PRIMER_LEFT_459_TM=57.165
PRIMER_RIGHT_459_TM=60.443
PRIMER_LEFT_459_GC_PERCENT=57.895
PRIMER_RIGHT_459_GC_PERCENT=43.478
PRIMER_LEFT_459_SELF_ANY=6.00
PRIMER_RIGHT_459_SELF_ANY=8.00
PRIMER_LEFT_459_SELF_END=3.00
PRIMER_RIGHT_459_SELF_END=2.00
PRIMER_LEFT_459_END_STABILITY=7.8000
PRIMER_RIGHT_459_END_STABILITY=6.4000
PRIMER_PAIR_459_COMPL_ANY=5.00
PRIMER_PAIR_459_COMPL_END=2.00
PRIMER_PAIR_459_PRODUCT_SIZE=88
PRIMER_PAIR_459_PRODUCT_TM=75.0
PRIMER_PAIR_460_PENALTY=7.321073
PRIMER_LEFT_460_PENALTY=3.428635
PRIMER_RIGHT_460_PENALTY=3.892438
PRIMER_LEFT_460_SEQUENCE=AGTGATGCCCTTCTCGTTCCT
PRIMER_RIGHT_460_SEQUENCE=CGGTGTGCCAGTGAAGAT
PRIMER_LEFT_460=569,21
PRIMER_RIGHT_460=656,18
PRIMER_LEFT_460_TM=62.429
PRIMER_RIGHT_460_TM=58.108
PRIMER_LEFT_460_GC_PERCENT=52.381
PRIMER_RIGHT_460_GC_PERCENT=55.556
PRIMER_LEFT_460_SELF_ANY=2.00
PRIMER_RIGHT_460_SELF_ANY=3.00
PRIMER_LEFT_460_SELF_END=0.00
PRIMER_RIGHT_460_SELF_END=2.00
PRIMER_LEFT_460_END_STABILITY=8.2000
PRIMER_RIGHT_460_END_STABILITY=6.6000
PRIMER_PAIR_460_COMPL_ANY=4.00
PRIMER_PAIR_460_COMPL_END=2.00
PRIMER_PAIR_460_PRODUCT_SIZE=88
PRIMER_PAIR_460_PRODUCT_TM=73.1
PRIMER_PAIR_461_PENALTY=7.353529
PRIMER_LEFT_461_PENALTY=4.457023
PRIMER_RIGHT_461_PENALTY=2.896506
PRIMER_LEFT_461_SEQUENCE=AAGCCAACCAACCAGTGA
PRIMER_RIGHT_461_SEQUENCE=CAGCGGTAGGGAATCACAAAG
PRIMER_LEFT_461=459,18
PRIMER_RIGHT_461=549,21
PRIMER_LEFT_461_TM=57.543
PRIMER_RIGHT_461_TM=61.897
PRIMER_LEFT_461_GC_PERCENT=50.000
PRIMER_RIGHT_461_GC_PERCENT=52.381
PRIMER_LEFT_461_SELF_ANY=3.00
PRIMER_RIGHT_461_SELF_ANY=3.00
PRIMER_LEFT_461_SELF_END=1.00
PRIMER_RIGHT_461_SELF_END=0.00
PRIMER_LEFT_461_END_STABILITY=6.4000
PRIMER_RIGHT_461_END_STABILITY=7.3000
PRIMER_PAIR_461_COMPL_ANY=4.00
PRIMER_PAIR_461_COMPL_END=1.00
PRIMER_PAIR_461_PRODUCT_SIZE=91
PRIMER_PAIR_461_PRODUCT_TM=77.2
PRIMER_PAIR_462_PENALTY=7.355960
PRIMER_LEFT_462_PENALTY=3.776506
PRIMER_RIGHT_462_PENALTY=3.579454
PRIMER_LEFT_462_SEQUENCE=ATCCCCACTTTGTGATTCCCTA
PRIMER_RIGHT_462_SEQUENCE=ATTTGCACTTGTCAGGAACGAG
PRIMER_LEFT_462=522,22
PRIMER_RIGHT_462=602,22
PRIMER_LEFT_462_TM=61.777
PRIMER_RIGHT_462_TM=61.579
PRIMER_LEFT_462_GC_PERCENT=45.455
PRIMER_RIGHT_462_GC_PERCENT=45.455
PRIMER_LEFT_462_SELF_ANY=4.00
PRIMER_RIGHT_462_SELF_ANY=4.00
PRIMER_LEFT_462_SELF_END=3.00
PRIMER_RIGHT_462_SELF_END=2.00
PRIMER_LEFT_462_END_STABILITY=8.7000
PRIMER_RIGHT_462_END_STABILITY=8.1000
PRIMER_PAIR_462_COMPL_ANY=4.00
PRIMER_PAIR_462_COMPL_END=1.00
PRIMER_PAIR_462_PRODUCT_SIZE=81
PRIMER_PAIR_462_PRODUCT_TM=71.7
PRIMER_PAIR_463_PENALTY=7.372506
PRIMER_LEFT_463_PENALTY=2.833316
PRIMER_RIGHT_463_PENALTY=4.539189
PRIMER_LEFT_463_SEQUENCE=CTTACACCAGGAGAGGATGG
PRIMER_RIGHT_463_SEQUENCE=GGTACTCTTCTCACTGCATGTCTC
PRIMER_LEFT_463=604,20
PRIMER_RIGHT_463=688,24
PRIMER_LEFT_463_TM=57.167
PRIMER_RIGHT_463_TM=59.461
PRIMER_LEFT_463_GC_PERCENT=55.000
PRIMER_RIGHT_463_GC_PERCENT=50.000
PRIMER_LEFT_463_SELF_ANY=3.00
PRIMER_RIGHT_463_SELF_ANY=4.00
PRIMER_LEFT_463_SELF_END=0.00
PRIMER_RIGHT_463_SELF_END=0.00
PRIMER_LEFT_463_END_STABILITY=8.1000
PRIMER_RIGHT_463_END_STABILITY=6.1000
PRIMER_PAIR_463_COMPL_ANY=6.00
PRIMER_PAIR_463_COMPL_END=3.00
PRIMER_PAIR_463_PRODUCT_SIZE=85
PRIMER_PAIR_463_PRODUCT_TM=73.6
PRIMER_PAIR_464_PENALTY=7.381861
PRIMER_LEFT_464_PENALTY=3.413777
PRIMER_RIGHT_464_PENALTY=3.968084
PRIMER_LEFT_464_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGT
PRIMER_RIGHT_464_SEQUENCE=TCGCAAACATCCATCCTC
PRIMER_LEFT_464=546,22
PRIMER_RIGHT_464=633,18
PRIMER_LEFT_464_TM=58.586
PRIMER_RIGHT_464_TM=58.032
PRIMER_LEFT_464_GC_PERCENT=45.455
PRIMER_RIGHT_464_GC_PERCENT=50.000
PRIMER_LEFT_464_SELF_ANY=3.00
PRIMER_RIGHT_464_SELF_ANY=2.00
PRIMER_LEFT_464_SELF_END=0.00
PRIMER_RIGHT_464_SELF_END=0.00
PRIMER_LEFT_464_END_STABILITY=7.0000
PRIMER_RIGHT_464_END_STABILITY=7.9000
PRIMER_PAIR_464_COMPL_ANY=5.00
PRIMER_PAIR_464_COMPL_END=2.00
PRIMER_PAIR_464_PRODUCT_SIZE=88
PRIMER_PAIR_464_PRODUCT_TM=72.2
PRIMER_PAIR_465_PENALTY=7.404682
PRIMER_LEFT_465_PENALTY=3.823109
PRIMER_RIGHT_465_PENALTY=3.581573
PRIMER_LEFT_465_SEQUENCE=ATCCCCACTTTGTGATTCC
PRIMER_RIGHT_465_SEQUENCE=GCACTTGTCAGGAACGAGA
PRIMER_LEFT_465=522,19
PRIMER_RIGHT_465=598,19
PRIMER_LEFT_465_TM=57.177
PRIMER_RIGHT_465_TM=57.418
PRIMER_LEFT_465_GC_PERCENT=47.368
PRIMER_RIGHT_465_GC_PERCENT=52.632
PRIMER_LEFT_465_SELF_ANY=4.00
PRIMER_RIGHT_465_SELF_ANY=3.00
PRIMER_LEFT_465_SELF_END=1.00
PRIMER_RIGHT_465_SELF_END=0.00
PRIMER_LEFT_465_END_STABILITY=8.1000
PRIMER_RIGHT_465_END_STABILITY=8.4000
PRIMER_PAIR_465_COMPL_ANY=4.00
PRIMER_PAIR_465_COMPL_END=3.00
PRIMER_PAIR_465_PRODUCT_SIZE=77
PRIMER_PAIR_465_PRODUCT_TM=72.3
PRIMER_PAIR_466_PENALTY=7.411188
PRIMER_LEFT_466_PENALTY=2.643243
PRIMER_RIGHT_466_PENALTY=4.767944
PRIMER_LEFT_466_SEQUENCE=AAACTCATCTTCACTGGCACA
PRIMER_RIGHT_466_SEQUENCE=GTCAATTCCGCAGGGCAG
PRIMER_LEFT_466=633,21
PRIMER_RIGHT_466=730,18
PRIMER_LEFT_466_TM=58.357
PRIMER_RIGHT_466_TM=62.768
PRIMER_LEFT_466_GC_PERCENT=42.857
PRIMER_RIGHT_466_GC_PERCENT=61.111
PRIMER_LEFT_466_SELF_ANY=3.00
PRIMER_RIGHT_466_SELF_ANY=4.00
PRIMER_LEFT_466_SELF_END=2.00
PRIMER_RIGHT_466_SELF_END=1.00
PRIMER_LEFT_466_END_STABILITY=8.2000
PRIMER_RIGHT_466_END_STABILITY=9.7000
PRIMER_PAIR_466_COMPL_ANY=4.00
PRIMER_PAIR_466_COMPL_END=2.00
PRIMER_PAIR_466_PRODUCT_SIZE=98
PRIMER_PAIR_466_PRODUCT_TM=74.7
PRIMER_PAIR_467_PENALTY=7.416007
PRIMER_LEFT_467_PENALTY=3.232712
PRIMER_RIGHT_467_PENALTY=4.183295
PRIMER_LEFT_467_SEQUENCE=AGTGCAAATTCTTACACCAGGA
PRIMER_RIGHT_467_SEQUENCE=GCATGTCTCTTTGGCGAC
PRIMER_LEFT_467=594,22
PRIMER_RIGHT_467=673,18
PRIMER_LEFT_467_TM=58.767
PRIMER_RIGHT_467_TM=57.817
PRIMER_LEFT_467_GC_PERCENT=40.909
PRIMER_RIGHT_467_GC_PERCENT=55.556
PRIMER_LEFT_467_SELF_ANY=5.00
PRIMER_RIGHT_467_SELF_ANY=4.00
PRIMER_LEFT_467_SELF_END=1.00
PRIMER_RIGHT_467_SELF_END=2.00
PRIMER_LEFT_467_END_STABILITY=8.2000
PRIMER_RIGHT_467_END_STABILITY=9.6000
PRIMER_PAIR_467_COMPL_ANY=4.00
PRIMER_PAIR_467_COMPL_END=0.00
PRIMER_PAIR_467_PRODUCT_SIZE=80
PRIMER_PAIR_467_PRODUCT_TM=72.4
PRIMER_PAIR_468_PENALTY=7.439162
PRIMER_LEFT_468_PENALTY=4.076046
PRIMER_RIGHT_468_PENALTY=3.363116
PRIMER_LEFT_468_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_468_SEQUENCE=CAGTTCTGGATGGTCACTGGTT
PRIMER_LEFT_468=411,24
PRIMER_RIGHT_468=489,22
PRIMER_LEFT_468_TM=60.076
PRIMER_RIGHT_468_TM=61.363
PRIMER_LEFT_468_GC_PERCENT=45.833
PRIMER_RIGHT_468_GC_PERCENT=50.000
PRIMER_LEFT_468_SELF_ANY=5.00
PRIMER_RIGHT_468_SELF_ANY=4.00
PRIMER_LEFT_468_SELF_END=1.00
PRIMER_RIGHT_468_SELF_END=0.00
PRIMER_LEFT_468_END_STABILITY=9.7000
PRIMER_RIGHT_468_END_STABILITY=8.2000
PRIMER_PAIR_468_COMPL_ANY=4.00
PRIMER_PAIR_468_COMPL_END=2.00
PRIMER_PAIR_468_PRODUCT_SIZE=79
PRIMER_PAIR_468_PRODUCT_TM=72.0
PRIMER_PAIR_469_PENALTY=7.454993
PRIMER_LEFT_469_PENALTY=4.397076
PRIMER_RIGHT_469_PENALTY=3.057917
PRIMER_LEFT_469_SEQUENCE=AGAACTGGTGCAAGCGGG
PRIMER_RIGHT_469_SEQUENCE=CACCAACTAAGCAGCGGTAGG
PRIMER_LEFT_469=483,18
PRIMER_RIGHT_469=560,21
PRIMER_LEFT_469_TM=62.397
PRIMER_RIGHT_469_TM=62.058
PRIMER_LEFT_469_GC_PERCENT=61.111
PRIMER_RIGHT_469_GC_PERCENT=57.143
PRIMER_LEFT_469_SELF_ANY=4.00
PRIMER_RIGHT_469_SELF_ANY=3.00
PRIMER_LEFT_469_SELF_END=0.00
PRIMER_RIGHT_469_SELF_END=1.00
PRIMER_LEFT_469_END_STABILITY=12.9000
PRIMER_RIGHT_469_END_STABILITY=6.9000
PRIMER_PAIR_469_COMPL_ANY=5.00
PRIMER_PAIR_469_COMPL_END=0.00
PRIMER_PAIR_469_PRODUCT_SIZE=78
PRIMER_PAIR_469_PRODUCT_TM=75.9
PRIMER_PAIR_470_PENALTY=7.459067
PRIMER_LEFT_470_PENALTY=4.149413
PRIMER_RIGHT_470_PENALTY=3.309654
PRIMER_LEFT_470_SEQUENCE=CAATGTGGTAGAAGCCAACCAA
PRIMER_RIGHT_470_SEQUENCE=GGGAATCACAAAGTGGGG
PRIMER_LEFT_470=448,22
PRIMER_RIGHT_470=541,18
PRIMER_LEFT_470_TM=62.149
PRIMER_RIGHT_470_TM=58.690
PRIMER_LEFT_470_GC_PERCENT=45.455
PRIMER_RIGHT_470_GC_PERCENT=55.556
PRIMER_LEFT_470_SELF_ANY=4.00
PRIMER_RIGHT_470_SELF_ANY=3.00
PRIMER_LEFT_470_SELF_END=2.00
PRIMER_RIGHT_470_SELF_END=0.00
PRIMER_LEFT_470_END_STABILITY=8.2000
PRIMER_RIGHT_470_END_STABILITY=11.2000
PRIMER_PAIR_470_COMPL_ANY=4.00
PRIMER_PAIR_470_COMPL_END=1.00
PRIMER_PAIR_470_PRODUCT_SIZE=94
PRIMER_PAIR_470_PRODUCT_TM=76.6
PRIMER_PAIR_471_PENALTY=7.468686
PRIMER_LEFT_471_PENALTY=3.477475
PRIMER_RIGHT_471_PENALTY=3.991211
PRIMER_LEFT_471_SEQUENCE=TACCGCTGCTTAGTTGGTG
PRIMER_RIGHT_471_SEQUENCE=CTCTCCTGGTGTAAGAATTTGC
PRIMER_LEFT_471=542,19
PRIMER_RIGHT_471=618,22
PRIMER_LEFT_471_TM=57.523
PRIMER_RIGHT_471_TM=58.009
PRIMER_LEFT_471_GC_PERCENT=52.632
PRIMER_RIGHT_471_GC_PERCENT=45.455
PRIMER_LEFT_471_SELF_ANY=3.00
PRIMER_RIGHT_471_SELF_ANY=4.00
PRIMER_LEFT_471_SELF_END=0.00
PRIMER_RIGHT_471_SELF_END=2.00
PRIMER_LEFT_471_END_STABILITY=8.2000
PRIMER_RIGHT_471_END_STABILITY=8.8000
PRIMER_PAIR_471_COMPL_ANY=4.00
PRIMER_PAIR_471_COMPL_END=1.00
PRIMER_PAIR_471_PRODUCT_SIZE=77
PRIMER_PAIR_471_PRODUCT_TM=71.3
PRIMER_PAIR_472_PENALTY=7.474878
PRIMER_LEFT_472_PENALTY=3.077802
PRIMER_RIGHT_472_PENALTY=4.397076
PRIMER_LEFT_472_SEQUENCE=GCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_472_SEQUENCE=CCCGCTTGCACCAGTTCT
PRIMER_LEFT_472=417,21
PRIMER_RIGHT_472=500,18
PRIMER_LEFT_472_TM=57.922
PRIMER_RIGHT_472_TM=62.397
PRIMER_LEFT_472_GC_PERCENT=52.381
PRIMER_RIGHT_472_GC_PERCENT=61.111
PRIMER_LEFT_472_SELF_ANY=4.00
PRIMER_RIGHT_472_SELF_ANY=4.00
PRIMER_LEFT_472_SELF_END=0.00
PRIMER_RIGHT_472_SELF_END=2.00
PRIMER_LEFT_472_END_STABILITY=6.7000
PRIMER_RIGHT_472_END_STABILITY=6.4000
PRIMER_PAIR_472_COMPL_ANY=4.00
PRIMER_PAIR_472_COMPL_END=3.00
PRIMER_PAIR_472_PRODUCT_SIZE=84
PRIMER_PAIR_472_PRODUCT_TM=74.7
PRIMER_PAIR_473_PENALTY=7.512983
PRIMER_LEFT_473_PENALTY=5.114578
PRIMER_RIGHT_473_PENALTY=2.398404
PRIMER_LEFT_473_SEQUENCE=AAAGAGACATGCAGTGAGAAGAG
PRIMER_RIGHT_473_SEQUENCE=CACAAACTCTACCCCTCGGAA
PRIMER_LEFT_473=662,23
PRIMER_RIGHT_473=754,21
PRIMER_LEFT_473_TM=57.885
PRIMER_RIGHT_473_TM=61.398
PRIMER_LEFT_473_GC_PERCENT=43.478
PRIMER_RIGHT_473_GC_PERCENT=52.381
PRIMER_LEFT_473_SELF_ANY=4.00
PRIMER_RIGHT_473_SELF_ANY=3.00
PRIMER_LEFT_473_SELF_END=0.00
PRIMER_RIGHT_473_SELF_END=1.00
PRIMER_LEFT_473_END_STABILITY=6.7000
PRIMER_RIGHT_473_END_STABILITY=10.2000
PRIMER_PAIR_473_COMPL_ANY=4.00
PRIMER_PAIR_473_COMPL_END=2.00
PRIMER_PAIR_473_PRODUCT_SIZE=93
PRIMER_PAIR_473_PRODUCT_TM=73.7
PRIMER_PAIR_474_PENALTY=7.527837
PRIMER_LEFT_474_PENALTY=4.457023
PRIMER_RIGHT_474_PENALTY=3.070814
PRIMER_LEFT_474_SEQUENCE=GAAGCCAACCAACCAGTG
PRIMER_RIGHT_474_SEQUENCE=TAAGCAGCGGTAGGGAATC
PRIMER_LEFT_474=458,18
PRIMER_RIGHT_474=553,19
PRIMER_LEFT_474_TM=57.543
PRIMER_RIGHT_474_TM=57.929
PRIMER_LEFT_474_GC_PERCENT=55.556
PRIMER_RIGHT_474_GC_PERCENT=52.632
PRIMER_LEFT_474_SELF_ANY=3.00
PRIMER_RIGHT_474_SELF_ANY=3.00
PRIMER_LEFT_474_SELF_END=3.00
PRIMER_RIGHT_474_SELF_END=3.00
PRIMER_LEFT_474_END_STABILITY=6.7000
PRIMER_RIGHT_474_END_STABILITY=6.6000
PRIMER_PAIR_474_COMPL_ANY=4.00
PRIMER_PAIR_474_COMPL_END=1.00
PRIMER_PAIR_474_PRODUCT_SIZE=96
PRIMER_PAIR_474_PRODUCT_TM=77.1
PRIMER_PAIR_475_PENALTY=7.531194
PRIMER_LEFT_475_PENALTY=2.880239
PRIMER_RIGHT_475_PENALTY=4.650955
PRIMER_LEFT_475_SEQUENCE=GGAGAGGATGGATGTTTGCG
PRIMER_RIGHT_475_SEQUENCE=GTCATGCAAGTTGGTACTCTTCTC
PRIMER_LEFT_475=613,20
PRIMER_RIGHT_475=700,24
PRIMER_LEFT_475_TM=62.880
PRIMER_RIGHT_475_TM=59.349
PRIMER_LEFT_475_GC_PERCENT=55.000
PRIMER_RIGHT_475_GC_PERCENT=45.833
PRIMER_LEFT_475_SELF_ANY=2.00
PRIMER_RIGHT_475_SELF_ANY=5.00
PRIMER_LEFT_475_SELF_END=2.00
PRIMER_RIGHT_475_SELF_END=0.00
PRIMER_LEFT_475_END_STABILITY=10.5000
PRIMER_RIGHT_475_END_STABILITY=6.7000
PRIMER_PAIR_475_COMPL_ANY=5.00
PRIMER_PAIR_475_COMPL_END=1.00
PRIMER_PAIR_475_PRODUCT_SIZE=88
PRIMER_PAIR_475_PRODUCT_TM=73.6
PRIMER_PAIR_476_PENALTY=7.533857
PRIMER_LEFT_476_PENALTY=3.904052
PRIMER_RIGHT_476_PENALTY=3.629805
PRIMER_LEFT_476_SEQUENCE=GACCCATCCCCACTTTGT
PRIMER_RIGHT_476_SEQUENCE=AGAATTTGCACTTGTCAGGAAC
PRIMER_LEFT_476=517,18
PRIMER_RIGHT_476=605,22
PRIMER_LEFT_476_TM=58.096
PRIMER_RIGHT_476_TM=58.370
PRIMER_LEFT_476_GC_PERCENT=55.556
PRIMER_RIGHT_476_GC_PERCENT=40.909
PRIMER_LEFT_476_SELF_ANY=2.00
PRIMER_RIGHT_476_SELF_ANY=4.00
PRIMER_LEFT_476_SELF_END=1.00
PRIMER_RIGHT_476_SELF_END=1.00
PRIMER_LEFT_476_END_STABILITY=7.0000
PRIMER_RIGHT_476_END_STABILITY=7.9000
PRIMER_PAIR_476_COMPL_ANY=3.00
PRIMER_PAIR_476_COMPL_END=2.00
PRIMER_PAIR_476_PRODUCT_SIZE=89
PRIMER_PAIR_476_PRODUCT_TM=73.0
PRIMER_PAIR_477_PENALTY=7.538089
PRIMER_LEFT_477_PENALTY=3.486268
PRIMER_RIGHT_477_PENALTY=4.051820
PRIMER_LEFT_477_SEQUENCE=CCGCTGCTTAGTTGGTGA
PRIMER_RIGHT_477_SEQUENCE=TTTCGCAAACATCCATCC
PRIMER_LEFT_477=544,18
PRIMER_RIGHT_477=635,18
PRIMER_LEFT_477_TM=58.514
PRIMER_RIGHT_477_TM=57.948
PRIMER_LEFT_477_GC_PERCENT=55.556
PRIMER_RIGHT_477_GC_PERCENT=44.444
PRIMER_LEFT_477_SELF_ANY=3.00
PRIMER_RIGHT_477_SELF_ANY=4.00
PRIMER_LEFT_477_SELF_END=1.00
PRIMER_RIGHT_477_SELF_END=0.00
PRIMER_LEFT_477_END_STABILITY=7.9000
PRIMER_RIGHT_477_END_STABILITY=8.1000
PRIMER_PAIR_477_COMPL_ANY=4.00
PRIMER_PAIR_477_COMPL_END=2.00
PRIMER_PAIR_477_PRODUCT_SIZE=92
PRIMER_PAIR_477_PRODUCT_TM=72.5
PRIMER_PAIR_478_PENALTY=7.552575
PRIMER_LEFT_478_PENALTY=3.918097
PRIMER_RIGHT_478_PENALTY=3.634478
PRIMER_LEFT_478_SEQUENCE=CAGATCACCAATGTGGTAGAAGC
PRIMER_RIGHT_478_SEQUENCE=AATCACAAAGTGGGGATGGGT
PRIMER_LEFT_478=440,23
PRIMER_RIGHT_478=538,21
PRIMER_LEFT_478_TM=60.918
PRIMER_RIGHT_478_TM=62.634
PRIMER_LEFT_478_GC_PERCENT=47.826
PRIMER_RIGHT_478_GC_PERCENT=47.619
PRIMER_LEFT_478_SELF_ANY=6.00
PRIMER_RIGHT_478_SELF_ANY=4.00
PRIMER_LEFT_478_SELF_END=2.00
PRIMER_RIGHT_478_SELF_END=0.00
PRIMER_LEFT_478_END_STABILITY=8.2000
PRIMER_RIGHT_478_END_STABILITY=9.4000
PRIMER_PAIR_478_COMPL_ANY=6.00
PRIMER_PAIR_478_COMPL_END=1.00
PRIMER_PAIR_478_PRODUCT_SIZE=99
PRIMER_PAIR_478_PRODUCT_TM=76.2
PRIMER_PAIR_479_PENALTY=7.552674
PRIMER_LEFT_479_PENALTY=3.874309
PRIMER_RIGHT_479_PENALTY=3.678365
PRIMER_LEFT_479_SEQUENCE=TGTGATTCCCTACCGCTG
PRIMER_RIGHT_479_SEQUENCE=TGTAAGAATTTGCACTTGTCAGG
PRIMER_LEFT_479=532,18
PRIMER_RIGHT_479=609,23
PRIMER_LEFT_479_TM=58.126
PRIMER_RIGHT_479_TM=59.322
PRIMER_LEFT_479_GC_PERCENT=55.556
PRIMER_RIGHT_479_GC_PERCENT=39.130
PRIMER_LEFT_479_SELF_ANY=3.00
PRIMER_RIGHT_479_SELF_ANY=4.00
PRIMER_LEFT_479_SELF_END=2.00
PRIMER_RIGHT_479_SELF_END=3.00
PRIMER_LEFT_479_END_STABILITY=10.2000
PRIMER_RIGHT_479_END_STABILITY=8.2000
PRIMER_PAIR_479_COMPL_ANY=4.00
PRIMER_PAIR_479_COMPL_END=2.00
PRIMER_PAIR_479_PRODUCT_SIZE=78
PRIMER_PAIR_479_PRODUCT_TM=70.6
PRIMER_PAIR_480_PENALTY=7.596215
PRIMER_LEFT_480_PENALTY=3.495754
PRIMER_RIGHT_480_PENALTY=4.100461
PRIMER_LEFT_480_SEQUENCE=TTTGTAAGTGATGCCCTTCTCG
PRIMER_RIGHT_480_SEQUENCE=GTGTGCCAGTGAAGATGAGTTTC
PRIMER_LEFT_480=563,22
PRIMER_RIGHT_480=654,23
PRIMER_LEFT_480_TM=61.496
PRIMER_RIGHT_480_TM=61.100
PRIMER_LEFT_480_GC_PERCENT=45.455
PRIMER_RIGHT_480_GC_PERCENT=47.826
PRIMER_LEFT_480_SELF_ANY=4.00
PRIMER_RIGHT_480_SELF_ANY=3.00
PRIMER_LEFT_480_SELF_END=2.00
PRIMER_RIGHT_480_SELF_END=3.00
PRIMER_LEFT_480_END_STABILITY=8.4000
PRIMER_RIGHT_480_END_STABILITY=6.7000
PRIMER_PAIR_480_COMPL_ANY=4.00
PRIMER_PAIR_480_COMPL_END=1.00
PRIMER_PAIR_480_PRODUCT_SIZE=92
PRIMER_PAIR_480_PRODUCT_TM=72.1
PRIMER_PAIR_481_PENALTY=7.599974
PRIMER_LEFT_481_PENALTY=3.790481
PRIMER_RIGHT_481_PENALTY=3.809493
PRIMER_LEFT_481_SEQUENCE=ATTCCCTACCGCTGCTTAGTTG
PRIMER_RIGHT_481_SEQUENCE=GGTGTAAGAATTTGCACTTGTCA
PRIMER_LEFT_481=536,22
PRIMER_RIGHT_481=611,23
PRIMER_LEFT_481_TM=61.790
PRIMER_RIGHT_481_TM=59.191
PRIMER_LEFT_481_GC_PERCENT=50.000
PRIMER_RIGHT_481_GC_PERCENT=39.130
PRIMER_LEFT_481_SELF_ANY=3.00
PRIMER_RIGHT_481_SELF_ANY=5.00
PRIMER_LEFT_481_SELF_END=1.00
PRIMER_RIGHT_481_SELF_END=3.00
PRIMER_LEFT_481_END_STABILITY=6.7000
PRIMER_RIGHT_481_END_STABILITY=6.7000
PRIMER_PAIR_481_COMPL_ANY=4.00
PRIMER_PAIR_481_COMPL_END=2.00
PRIMER_PAIR_481_PRODUCT_SIZE=76
PRIMER_PAIR_481_PRODUCT_TM=70.9
PRIMER_PAIR_482_PENALTY=7.619819
PRIMER_LEFT_482_PENALTY=3.711172
PRIMER_RIGHT_482_PENALTY=3.908647
PRIMER_LEFT_482_SEQUENCE=CCCTACCGCTGCTTAGTTGGT
PRIMER_RIGHT_482_SEQUENCE=CCTGGTGTAAGAATTTGCACTTG
PRIMER_LEFT_482=539,21
PRIMER_RIGHT_482=614,23
PRIMER_LEFT_482_TM=62.711
PRIMER_RIGHT_482_TM=60.909
PRIMER_LEFT_482_GC_PERCENT=57.143
PRIMER_RIGHT_482_GC_PERCENT=43.478
PRIMER_LEFT_482_SELF_ANY=3.00
PRIMER_RIGHT_482_SELF_ANY=5.00
PRIMER_LEFT_482_SELF_END=0.00
PRIMER_RIGHT_482_SELF_END=3.00
PRIMER_LEFT_482_END_STABILITY=8.2000
PRIMER_RIGHT_482_END_STABILITY=6.7000
PRIMER_PAIR_482_COMPL_ANY=4.00
PRIMER_PAIR_482_COMPL_END=0.00
PRIMER_PAIR_482_PRODUCT_SIZE=76
PRIMER_PAIR_482_PRODUCT_TM=72.0
PRIMER_PAIR_483_PENALTY=7.651212
PRIMER_LEFT_483_PENALTY=3.527826
PRIMER_RIGHT_483_PENALTY=4.123386
PRIMER_LEFT_483_SEQUENCE=TGAGTTTGTAAGTGATGCCCTTC
PRIMER_RIGHT_483_SEQUENCE=GTGCCAGTGAAGATGAGTTTCG
PRIMER_LEFT_483=559,23
PRIMER_RIGHT_483=652,22
PRIMER_LEFT_483_TM=60.528
PRIMER_RIGHT_483_TM=62.123
PRIMER_LEFT_483_GC_PERCENT=43.478
PRIMER_RIGHT_483_GC_PERCENT=50.000
PRIMER_LEFT_483_SELF_ANY=4.00
PRIMER_RIGHT_483_SELF_ANY=3.00
PRIMER_LEFT_483_SELF_END=2.00
PRIMER_RIGHT_483_SELF_END=2.00
PRIMER_LEFT_483_END_STABILITY=8.2000
PRIMER_RIGHT_483_END_STABILITY=9.0000
PRIMER_PAIR_483_COMPL_ANY=4.00
PRIMER_PAIR_483_COMPL_END=1.00
PRIMER_PAIR_483_PRODUCT_SIZE=94
PRIMER_PAIR_483_PRODUCT_TM=72.3
PRIMER_PAIR_484_PENALTY=7.656499
PRIMER_LEFT_484_PENALTY=2.936750
PRIMER_RIGHT_484_PENALTY=4.719749
PRIMER_LEFT_484_SEQUENCE=CAGGAGAGGATGGATGTTTGC
PRIMER_RIGHT_484_SEQUENCE=TTGGTACTCTTCTCACTGCATGTC
PRIMER_LEFT_484=611,21
PRIMER_RIGHT_484=690,24
PRIMER_LEFT_484_TM=61.937
PRIMER_RIGHT_484_TM=60.720
PRIMER_LEFT_484_GC_PERCENT=52.381
PRIMER_RIGHT_484_GC_PERCENT=45.833
PRIMER_LEFT_484_SELF_ANY=2.00
PRIMER_RIGHT_484_SELF_ANY=4.00
PRIMER_LEFT_484_SELF_END=2.00
PRIMER_RIGHT_484_SELF_END=2.00
PRIMER_LEFT_484_END_STABILITY=8.8000
PRIMER_RIGHT_484_END_STABILITY=6.3000
PRIMER_PAIR_484_COMPL_ANY=6.00
PRIMER_PAIR_484_COMPL_END=0.00
PRIMER_PAIR_484_PRODUCT_SIZE=80
PRIMER_PAIR_484_PRODUCT_TM=72.9
PRIMER_PAIR_485_PENALTY=7.656680
PRIMER_LEFT_485_PENALTY=5.256192
PRIMER_RIGHT_485_PENALTY=2.400488
PRIMER_LEFT_485_SEQUENCE=AAAGAGACATGCAGTGAGAAGAGT
PRIMER_RIGHT_485_SEQUENCE=AACTCTACCCCTCGGAACTTGT
PRIMER_LEFT_485=662,24
PRIMER_RIGHT_485=750,22
PRIMER_LEFT_485_TM=58.744
PRIMER_RIGHT_485_TM=60.400
PRIMER_LEFT_485_GC_PERCENT=41.667
PRIMER_RIGHT_485_GC_PERCENT=50.000
PRIMER_LEFT_485_SELF_ANY=4.00
PRIMER_RIGHT_485_SELF_ANY=3.00
PRIMER_LEFT_485_SELF_END=1.00
PRIMER_RIGHT_485_SELF_END=2.00
PRIMER_LEFT_485_END_STABILITY=6.1000
PRIMER_RIGHT_485_END_STABILITY=6.7000
PRIMER_PAIR_485_COMPL_ANY=5.00
PRIMER_PAIR_485_COMPL_END=2.00
PRIMER_PAIR_485_PRODUCT_SIZE=89
PRIMER_PAIR_485_PRODUCT_TM=73.4
PRIMER_PAIR_486_PENALTY=7.656948
PRIMER_LEFT_486_PENALTY=3.918097
PRIMER_RIGHT_486_PENALTY=3.738851
PRIMER_LEFT_486_SEQUENCE=GCAGATCACCAATGTGGTAGAAG
PRIMER_RIGHT_486_SEQUENCE=AAGTGGGGATGGGTCTTG
PRIMER_LEFT_486=439,23
PRIMER_RIGHT_486=531,18
PRIMER_LEFT_486_TM=60.918
PRIMER_RIGHT_486_TM=58.261
PRIMER_LEFT_486_GC_PERCENT=47.826
PRIMER_RIGHT_486_GC_PERCENT=55.556
PRIMER_LEFT_486_SELF_ANY=6.00
PRIMER_RIGHT_486_SELF_ANY=3.00
PRIMER_LEFT_486_SELF_END=2.00
PRIMER_RIGHT_486_SELF_END=0.00
PRIMER_LEFT_486_END_STABILITY=6.7000
PRIMER_RIGHT_486_END_STABILITY=7.0000
PRIMER_PAIR_486_COMPL_ANY=5.00
PRIMER_PAIR_486_COMPL_END=2.00
PRIMER_PAIR_486_PRODUCT_SIZE=93
PRIMER_PAIR_486_PRODUCT_TM=76.8
PRIMER_PAIR_487_PENALTY=7.730448
PRIMER_LEFT_487_PENALTY=3.920955
PRIMER_RIGHT_487_PENALTY=3.809493
PRIMER_LEFT_487_SEQUENCE=ATTCCCTACCGCTGCTTAG
PRIMER_RIGHT_487_SEQUENCE=TGGTGTAAGAATTTGCACTTGTC
PRIMER_LEFT_487=536,19
PRIMER_RIGHT_487=612,23
PRIMER_LEFT_487_TM=57.079
PRIMER_RIGHT_487_TM=59.191
PRIMER_LEFT_487_GC_PERCENT=52.632
PRIMER_RIGHT_487_GC_PERCENT=39.130
PRIMER_LEFT_487_SELF_ANY=3.00
PRIMER_RIGHT_487_SELF_ANY=5.00
PRIMER_LEFT_487_SELF_END=3.00
PRIMER_RIGHT_487_SELF_END=2.00
PRIMER_LEFT_487_END_STABILITY=6.0000
PRIMER_RIGHT_487_END_STABILITY=6.7000
PRIMER_PAIR_487_COMPL_ANY=4.00
PRIMER_PAIR_487_COMPL_END=1.00
PRIMER_PAIR_487_PRODUCT_SIZE=77
PRIMER_PAIR_487_PRODUCT_TM=70.7
PRIMER_PAIR_488_PENALTY=7.771286
PRIMER_LEFT_488_PENALTY=2.908522
PRIMER_RIGHT_488_PENALTY=4.862764
PRIMER_LEFT_488_SEQUENCE=TCATCTTCACTGGCACACC
PRIMER_RIGHT_488_SEQUENCE=ACTTGTCAATTCCGCAGG
PRIMER_LEFT_488=637,19
PRIMER_RIGHT_488=734,18
PRIMER_LEFT_488_TM=58.091
PRIMER_RIGHT_488_TM=57.137
PRIMER_LEFT_488_GC_PERCENT=52.632
PRIMER_RIGHT_488_GC_PERCENT=50.000
PRIMER_LEFT_488_SELF_ANY=3.00
PRIMER_RIGHT_488_SELF_ANY=5.00
PRIMER_LEFT_488_SELF_END=0.00
PRIMER_RIGHT_488_SELF_END=2.00
PRIMER_LEFT_488_END_STABILITY=7.6000
PRIMER_RIGHT_488_END_STABILITY=9.7000
PRIMER_PAIR_488_COMPL_ANY=3.00
PRIMER_PAIR_488_COMPL_END=2.00
PRIMER_PAIR_488_PRODUCT_SIZE=98
PRIMER_PAIR_488_PRODUCT_TM=74.7
PRIMER_PAIR_489_PENALTY=7.803762
PRIMER_LEFT_489_PENALTY=3.004178
PRIMER_RIGHT_489_PENALTY=4.799584
PRIMER_LEFT_489_SEQUENCE=GGAGAGGATGGATGTTTGC
PRIMER_RIGHT_489_SEQUENCE=CAAGTTGGTACTCTTCTCACTGC
PRIMER_LEFT_489=613,19
PRIMER_RIGHT_489=694,23
PRIMER_LEFT_489_TM=57.996
PRIMER_RIGHT_489_TM=58.200
PRIMER_LEFT_489_GC_PERCENT=52.632
PRIMER_RIGHT_489_GC_PERCENT=47.826
PRIMER_LEFT_489_SELF_ANY=2.00
PRIMER_RIGHT_489_SELF_ANY=5.00
PRIMER_LEFT_489_SELF_END=2.00
PRIMER_RIGHT_489_SELF_END=2.00
PRIMER_LEFT_489_END_STABILITY=8.8000
PRIMER_RIGHT_489_END_STABILITY=7.9000
PRIMER_PAIR_489_COMPL_ANY=5.00
PRIMER_PAIR_489_COMPL_END=2.00
PRIMER_PAIR_489_PRODUCT_SIZE=82
PRIMER_PAIR_489_PRODUCT_TM=73.1
PRIMER_PAIR_490_PENALTY=7.845623
PRIMER_LEFT_490_PENALTY=4.812325
PRIMER_RIGHT_490_PENALTY=3.033298
PRIMER_LEFT_490_SEQUENCE=AGCAGTGCAAGACCCATC
PRIMER_RIGHT_490_SEQUENCE=ACGAGAAGGGCATCACTTACAA
PRIMER_LEFT_490=507,18
PRIMER_RIGHT_490=585,22
PRIMER_LEFT_490_TM=57.188
PRIMER_RIGHT_490_TM=61.033
PRIMER_LEFT_490_GC_PERCENT=55.556
PRIMER_RIGHT_490_GC_PERCENT=45.455
PRIMER_LEFT_490_SELF_ANY=5.00
PRIMER_RIGHT_490_SELF_ANY=4.00
PRIMER_LEFT_490_SELF_END=0.00
PRIMER_RIGHT_490_SELF_END=2.00
PRIMER_LEFT_490_END_STABILITY=8.1000
PRIMER_RIGHT_490_END_STABILITY=6.0000
PRIMER_PAIR_490_COMPL_ANY=5.00
PRIMER_PAIR_490_COMPL_END=2.00
PRIMER_PAIR_490_PRODUCT_SIZE=79
PRIMER_PAIR_490_PRODUCT_TM=73.1
PRIMER_PAIR_491_PENALTY=7.857702
PRIMER_LEFT_491_PENALTY=3.258282
PRIMER_RIGHT_491_PENALTY=4.599420
PRIMER_LEFT_491_SEQUENCE=GTGCAAATTCTTACACCAGGA
PRIMER_RIGHT_491_SEQUENCE=CTCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_491=595,21
PRIMER_RIGHT_491=684,24
PRIMER_LEFT_491_TM=57.742
PRIMER_RIGHT_491_TM=60.599
PRIMER_LEFT_491_GC_PERCENT=42.857
PRIMER_RIGHT_491_GC_PERCENT=45.833
PRIMER_LEFT_491_SELF_ANY=5.00
PRIMER_RIGHT_491_SELF_ANY=4.00
PRIMER_LEFT_491_SELF_END=1.00
PRIMER_RIGHT_491_SELF_END=0.00
PRIMER_LEFT_491_END_STABILITY=8.2000
PRIMER_RIGHT_491_END_STABILITY=7.3000
PRIMER_PAIR_491_COMPL_ANY=4.00
PRIMER_PAIR_491_COMPL_END=2.00
PRIMER_PAIR_491_PRODUCT_SIZE=90
PRIMER_PAIR_491_PRODUCT_TM=73.3
PRIMER_PAIR_492_PENALTY=7.867014
PRIMER_LEFT_492_PENALTY=2.207202
PRIMER_RIGHT_492_PENALTY=5.659812
PRIMER_LEFT_492_SEQUENCE=GAATGGGAAGTGGGATTCAGA
PRIMER_RIGHT_492_SEQUENCE=CTGCAGTTCAGGGTAGACTTCTTG
PRIMER_LEFT_492=343,21
PRIMER_RIGHT_492=442,24
PRIMER_LEFT_492_TM=61.207
PRIMER_RIGHT_492_TM=61.660
PRIMER_LEFT_492_GC_PERCENT=47.619
PRIMER_RIGHT_492_GC_PERCENT=50.000
PRIMER_LEFT_492_SELF_ANY=4.00
PRIMER_RIGHT_492_SELF_ANY=6.00
PRIMER_LEFT_492_SELF_END=3.00
PRIMER_RIGHT_492_SELF_END=1.00
PRIMER_LEFT_492_END_STABILITY=6.7000
PRIMER_RIGHT_492_END_STABILITY=7.0000
PRIMER_PAIR_492_COMPL_ANY=5.00
PRIMER_PAIR_492_COMPL_END=2.00
PRIMER_PAIR_492_PRODUCT_SIZE=100
PRIMER_PAIR_492_PRODUCT_TM=74.0
PRIMER_PAIR_493_PENALTY=7.998550
PRIMER_LEFT_493_PENALTY=3.123340
PRIMER_RIGHT_493_PENALTY=4.875209
PRIMER_LEFT_493_SEQUENCE=ATGCACATGAATGTCCAGAAT
PRIMER_RIGHT_493_SEQUENCE=TTCCTTGGTATCAATGCAGGTTT
PRIMER_LEFT_493=326,21
PRIMER_RIGHT_493=400,23
PRIMER_LEFT_493_TM=57.877
PRIMER_RIGHT_493_TM=61.875
PRIMER_LEFT_493_GC_PERCENT=38.095
PRIMER_RIGHT_493_GC_PERCENT=39.130
PRIMER_LEFT_493_SELF_ANY=8.00
PRIMER_RIGHT_493_SELF_ANY=6.00
PRIMER_LEFT_493_SELF_END=2.00
PRIMER_RIGHT_493_SELF_END=1.00
PRIMER_LEFT_493_END_STABILITY=6.6000
PRIMER_RIGHT_493_END_STABILITY=8.2000
PRIMER_PAIR_493_COMPL_ANY=4.00
PRIMER_PAIR_493_COMPL_END=1.00
PRIMER_PAIR_493_PRODUCT_SIZE=75
PRIMER_PAIR_493_PRODUCT_TM=70.5
PRIMER_PAIR_494_PENALTY=8.009088
PRIMER_LEFT_494_PENALTY=4.320176
PRIMER_RIGHT_494_PENALTY=3.688912
PRIMER_LEFT_494_SEQUENCE=TCCCCACTTTGTGATTCCCTAC
PRIMER_RIGHT_494_SEQUENCE=GAATTTGCACTTGTCAGGAAC
PRIMER_LEFT_494=523,22
PRIMER_RIGHT_494=604,21
PRIMER_LEFT_494_TM=62.320
PRIMER_RIGHT_494_TM=57.311
PRIMER_LEFT_494_GC_PERCENT=50.000
PRIMER_RIGHT_494_GC_PERCENT=42.857
PRIMER_LEFT_494_SELF_ANY=3.00
PRIMER_RIGHT_494_SELF_ANY=4.00
PRIMER_LEFT_494_SELF_END=1.00
PRIMER_RIGHT_494_SELF_END=1.00
PRIMER_LEFT_494_END_STABILITY=6.9000
PRIMER_RIGHT_494_END_STABILITY=7.9000
PRIMER_PAIR_494_COMPL_ANY=4.00
PRIMER_PAIR_494_COMPL_END=3.00
PRIMER_PAIR_494_PRODUCT_SIZE=82
PRIMER_PAIR_494_PRODUCT_TM=72.1
PRIMER_PAIR_495_PENALTY=8.018685
PRIMER_LEFT_495_PENALTY=4.985387
PRIMER_RIGHT_495_PENALTY=3.033298
PRIMER_LEFT_495_SEQUENCE=TGCAAGACCCATCCCCAC
PRIMER_RIGHT_495_SEQUENCE=AACGAGAAGGGCATCACTTACA
PRIMER_LEFT_495=512,18
PRIMER_RIGHT_495=586,22
PRIMER_LEFT_495_TM=62.985
PRIMER_RIGHT_495_TM=61.033
PRIMER_LEFT_495_GC_PERCENT=61.111
PRIMER_RIGHT_495_GC_PERCENT=45.455
PRIMER_LEFT_495_SELF_ANY=4.00
PRIMER_RIGHT_495_SELF_ANY=4.00
PRIMER_LEFT_495_SELF_END=0.00
PRIMER_RIGHT_495_SELF_END=0.00
PRIMER_LEFT_495_END_STABILITY=9.4000
PRIMER_RIGHT_495_END_STABILITY=6.0000
PRIMER_PAIR_495_COMPL_ANY=4.00
PRIMER_PAIR_495_COMPL_END=0.00
PRIMER_PAIR_495_PRODUCT_SIZE=75
PRIMER_PAIR_495_PRODUCT_TM=72.1
PRIMER_PAIR_496_PENALTY=8.033437
PRIMER_LEFT_496_PENALTY=6.544143
PRIMER_RIGHT_496_PENALTY=1.489294
PRIMER_LEFT_496_SEQUENCE=AAGAGACATGCAGTGAGAAGAGTA
PRIMER_RIGHT_496_SEQUENCE=GGGCAACACACAAACTCTACC
PRIMER_LEFT_496=663,24
PRIMER_RIGHT_496=762,21
PRIMER_LEFT_496_TM=57.456
PRIMER_RIGHT_496_TM=59.511
PRIMER_LEFT_496_GC_PERCENT=41.667
PRIMER_RIGHT_496_GC_PERCENT=52.381
PRIMER_LEFT_496_SELF_ANY=4.00
PRIMER_RIGHT_496_SELF_ANY=2.00
PRIMER_LEFT_496_SELF_END=2.00
PRIMER_RIGHT_496_SELF_END=0.00
PRIMER_LEFT_496_END_STABILITY=5.4000
PRIMER_RIGHT_496_END_STABILITY=6.9000
PRIMER_PAIR_496_COMPL_ANY=5.00
PRIMER_PAIR_496_COMPL_END=3.00
PRIMER_PAIR_496_PRODUCT_SIZE=100
PRIMER_PAIR_496_PRODUCT_TM=74.8
PRIMER_PAIR_497_PENALTY=8.052866
PRIMER_LEFT_497_PENALTY=5.652377
PRIMER_RIGHT_497_PENALTY=2.400488
PRIMER_LEFT_497_SEQUENCE=AAGAGACATGCAGTGAGAAGAGT
PRIMER_RIGHT_497_SEQUENCE=ACAAACTCTACCCCTCGGAACT
PRIMER_LEFT_497=663,23
PRIMER_RIGHT_497=753,22
PRIMER_LEFT_497_TM=57.348
PRIMER_RIGHT_497_TM=60.400
PRIMER_LEFT_497_GC_PERCENT=43.478
PRIMER_RIGHT_497_GC_PERCENT=50.000
PRIMER_LEFT_497_SELF_ANY=4.00
PRIMER_RIGHT_497_SELF_ANY=3.00
PRIMER_LEFT_497_SELF_END=1.00
PRIMER_RIGHT_497_SELF_END=1.00
PRIMER_LEFT_497_END_STABILITY=6.1000
PRIMER_RIGHT_497_END_STABILITY=6.4000
PRIMER_PAIR_497_COMPL_ANY=5.00
PRIMER_PAIR_497_COMPL_END=3.00
PRIMER_PAIR_497_PRODUCT_SIZE=91
PRIMER_PAIR_497_PRODUCT_TM=73.6
PRIMER_PAIR_498_PENALTY=8.056021
PRIMER_LEFT_498_PENALTY=3.256437
PRIMER_RIGHT_498_PENALTY=4.799584
PRIMER_LEFT_498_SEQUENCE=TTACACCAGGAGAGGATGGATG
PRIMER_RIGHT_498_SEQUENCE=GCAAGTTGGTACTCTTCTCACTG
PRIMER_LEFT_498=605,22
PRIMER_RIGHT_498=695,23
PRIMER_LEFT_498_TM=61.256
PRIMER_RIGHT_498_TM=58.200
PRIMER_LEFT_498_GC_PERCENT=50.000
PRIMER_RIGHT_498_GC_PERCENT=47.826
PRIMER_LEFT_498_SELF_ANY=3.00
PRIMER_RIGHT_498_SELF_ANY=5.00
PRIMER_LEFT_498_SELF_END=0.00
PRIMER_RIGHT_498_SELF_END=3.00
PRIMER_LEFT_498_END_STABILITY=8.1000
PRIMER_RIGHT_498_END_STABILITY=6.7000
PRIMER_PAIR_498_COMPL_ANY=6.00
PRIMER_PAIR_498_COMPL_END=3.00
PRIMER_PAIR_498_PRODUCT_SIZE=91
PRIMER_PAIR_498_PRODUCT_TM=73.6
PRIMER_PAIR_499_PENALTY=8.090730
PRIMER_LEFT_499_PENALTY=5.604473
PRIMER_RIGHT_499_PENALTY=2.486257
PRIMER_LEFT_499_SEQUENCE=AACATGCACATGAATGTCCAGAA
PRIMER_RIGHT_499_SEQUENCE=CTTGGTATCAATGCAGGTTTTG
PRIMER_LEFT_499=323,23
PRIMER_RIGHT_499=397,22
PRIMER_LEFT_499_TM=62.604
PRIMER_RIGHT_499_TM=59.514
PRIMER_LEFT_499_GC_PERCENT=39.130
PRIMER_RIGHT_499_GC_PERCENT=40.909
PRIMER_LEFT_499_SELF_ANY=8.00
PRIMER_RIGHT_499_SELF_ANY=6.00
PRIMER_LEFT_499_SELF_END=2.00
PRIMER_RIGHT_499_SELF_END=2.00
PRIMER_LEFT_499_END_STABILITY=7.0000
PRIMER_RIGHT_499_END_STABILITY=7.6000
PRIMER_PAIR_499_COMPL_ANY=6.00
PRIMER_PAIR_499_COMPL_END=3.00
PRIMER_PAIR_499_PRODUCT_SIZE=75
PRIMER_PAIR_499_PRODUCT_TM=70.5
PRIMER_PAIR_500_PENALTY=8.120276
PRIMER_LEFT_500_PENALTY=3.296517
PRIMER_RIGHT_500_PENALTY=4.823759
PRIMER_LEFT_500_SEQUENCE=AGTGCAAATTCTTACACCAGGAG
PRIMER_RIGHT_500_SEQUENCE=TCTCACTGCATGTCTCTTTGGC
PRIMER_LEFT_500=594,23
PRIMER_RIGHT_500=680,22
PRIMER_LEFT_500_TM=59.703
PRIMER_RIGHT_500_TM=62.824
PRIMER_LEFT_500_GC_PERCENT=43.478
PRIMER_RIGHT_500_GC_PERCENT=50.000
PRIMER_LEFT_500_SELF_ANY=5.00
PRIMER_RIGHT_500_SELF_ANY=4.00
PRIMER_LEFT_500_SELF_END=3.00
PRIMER_RIGHT_500_SELF_END=2.00
PRIMER_LEFT_500_END_STABILITY=7.9000
PRIMER_RIGHT_500_END_STABILITY=10.0000
PRIMER_PAIR_500_COMPL_ANY=4.00
PRIMER_PAIR_500_COMPL_END=2.00
PRIMER_PAIR_500_PRODUCT_SIZE=87
PRIMER_PAIR_500_PRODUCT_TM=72.8
PRIMER_PAIR_501_PENALTY=8.143311
PRIMER_LEFT_501_PENALTY=4.366422
PRIMER_RIGHT_501_PENALTY=3.776889
PRIMER_LEFT_501_SEQUENCE=ACCAATGTGGTAGAAGCCAACC
PRIMER_RIGHT_501_SEQUENCE=GGTCTTGCACTGCTTGCG
PRIMER_LEFT_501=446,22
PRIMER_RIGHT_501=520,18
PRIMER_LEFT_501_TM=62.366
PRIMER_RIGHT_501_TM=61.777
PRIMER_LEFT_501_GC_PERCENT=50.000
PRIMER_RIGHT_501_GC_PERCENT=61.111
PRIMER_LEFT_501_SELF_ANY=6.00
PRIMER_RIGHT_501_SELF_ANY=5.00
PRIMER_LEFT_501_SELF_END=2.00
PRIMER_RIGHT_501_SELF_END=2.00
PRIMER_LEFT_501_END_STABILITY=8.2000
PRIMER_RIGHT_501_END_STABILITY=10.5000
PRIMER_PAIR_501_COMPL_ANY=4.00
PRIMER_PAIR_501_COMPL_END=2.00
PRIMER_PAIR_501_PRODUCT_SIZE=75
PRIMER_PAIR_501_PRODUCT_TM=75.4
PRIMER_PAIR_502_PENALTY=8.145270
PRIMER_LEFT_502_PENALTY=3.655999
PRIMER_RIGHT_502_PENALTY=4.489271
PRIMER_LEFT_502_SEQUENCE=TGAGTTTGTAAGTGATGCCCT
PRIMER_RIGHT_502_SEQUENCE=TTCGCAAACATCCATCCT
PRIMER_LEFT_502=559,21
PRIMER_RIGHT_502=634,18
PRIMER_LEFT_502_TM=57.344
PRIMER_RIGHT_502_TM=57.511
PRIMER_LEFT_502_GC_PERCENT=42.857
PRIMER_RIGHT_502_GC_PERCENT=44.444
PRIMER_LEFT_502_SELF_ANY=2.00
PRIMER_RIGHT_502_SELF_ANY=4.00
PRIMER_LEFT_502_SELF_END=2.00
PRIMER_RIGHT_502_SELF_END=0.00
PRIMER_LEFT_502_END_STABILITY=10.9000
PRIMER_RIGHT_502_END_STABILITY=7.8000
PRIMER_PAIR_502_COMPL_ANY=5.00
PRIMER_PAIR_502_COMPL_END=1.00
PRIMER_PAIR_502_PRODUCT_SIZE=76
PRIMER_PAIR_502_PRODUCT_TM=70.4
PRIMER_PAIR_503_PENALTY=8.156303
PRIMER_LEFT_503_PENALTY=4.399603
PRIMER_RIGHT_503_PENALTY=3.756700
PRIMER_LEFT_503_SEQUENCE=TGCAGTATTGCCAAGAAGTCTAC
PRIMER_RIGHT_503_SEQUENCE=TCTGGATGGTCACTGGTTGGT
PRIMER_LEFT_503=408,23
PRIMER_RIGHT_503=485,21
PRIMER_LEFT_503_TM=58.600
PRIMER_RIGHT_503_TM=62.757
PRIMER_LEFT_503_GC_PERCENT=43.478
PRIMER_RIGHT_503_GC_PERCENT=52.381
PRIMER_LEFT_503_SELF_ANY=6.00
PRIMER_RIGHT_503_SELF_ANY=3.00
PRIMER_LEFT_503_SELF_END=3.00
PRIMER_RIGHT_503_SELF_END=0.00
PRIMER_LEFT_503_END_STABILITY=5.4000
PRIMER_RIGHT_503_END_STABILITY=8.2000
PRIMER_PAIR_503_COMPL_ANY=5.00
PRIMER_PAIR_503_COMPL_END=3.00
PRIMER_PAIR_503_PRODUCT_SIZE=78
PRIMER_PAIR_503_PRODUCT_TM=72.2
PRIMER_PAIR_504_PENALTY=8.156360
PRIMER_LEFT_504_PENALTY=3.161374
PRIMER_RIGHT_504_PENALTY=4.994987
PRIMER_LEFT_504_SEQUENCE=GATGTTTGCGAAACTCATCTTC
PRIMER_RIGHT_504_SEQUENCE=CGTAGTCATGCAAGTTGGTACT
PRIMER_LEFT_504=623,22
PRIMER_RIGHT_504=704,22
PRIMER_LEFT_504_TM=58.839
PRIMER_RIGHT_504_TM=57.005
PRIMER_LEFT_504_GC_PERCENT=40.909
PRIMER_RIGHT_504_GC_PERCENT=45.455
PRIMER_LEFT_504_SELF_ANY=6.00
PRIMER_RIGHT_504_SELF_ANY=5.00
PRIMER_LEFT_504_SELF_END=3.00
PRIMER_RIGHT_504_SELF_END=3.00
PRIMER_LEFT_504_END_STABILITY=6.7000
PRIMER_RIGHT_504_END_STABILITY=5.1000
PRIMER_PAIR_504_COMPL_ANY=4.00
PRIMER_PAIR_504_COMPL_END=1.00
PRIMER_PAIR_504_PRODUCT_SIZE=82
PRIMER_PAIR_504_PRODUCT_TM=72.6
PRIMER_PAIR_505_PENALTY=8.165522
PRIMER_LEFT_505_PENALTY=4.705093
PRIMER_RIGHT_505_PENALTY=3.460430
PRIMER_LEFT_505_SEQUENCE=GGAGGTACCCACTGATGGTAATG
PRIMER_RIGHT_505_SEQUENCE=ACATTCATGTGCATGTTCAGTCT
PRIMER_LEFT_505=253,23
PRIMER_RIGHT_505=339,23
PRIMER_LEFT_505_TM=61.705
PRIMER_RIGHT_505_TM=59.540
PRIMER_LEFT_505_GC_PERCENT=52.174
PRIMER_RIGHT_505_GC_PERCENT=39.130
PRIMER_LEFT_505_SELF_ANY=6.00
PRIMER_RIGHT_505_SELF_ANY=8.00
PRIMER_LEFT_505_SELF_END=0.00
PRIMER_RIGHT_505_SELF_END=3.00
PRIMER_LEFT_505_END_STABILITY=6.2000
PRIMER_RIGHT_505_END_STABILITY=6.1000
PRIMER_PAIR_505_COMPL_ANY=5.00
PRIMER_PAIR_505_COMPL_END=1.00
PRIMER_PAIR_505_PRODUCT_SIZE=87
PRIMER_PAIR_505_PRODUCT_TM=74.7
PRIMER_PAIR_506_PENALTY=8.171832
PRIMER_LEFT_506_PENALTY=4.111908
PRIMER_RIGHT_506_PENALTY=4.059923
PRIMER_LEFT_506_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTAA
PRIMER_RIGHT_506_SEQUENCE=AACATCCATCCTCTCCTGGTGT
PRIMER_LEFT_506=546,24
PRIMER_RIGHT_506=628,22
PRIMER_LEFT_506_TM=59.888
PRIMER_RIGHT_506_TM=62.060
PRIMER_LEFT_506_GC_PERCENT=41.667
PRIMER_RIGHT_506_GC_PERCENT=50.000
PRIMER_LEFT_506_SELF_ANY=3.00
PRIMER_RIGHT_506_SELF_ANY=4.00
PRIMER_LEFT_506_SELF_END=2.00
PRIMER_RIGHT_506_SELF_END=1.00
PRIMER_LEFT_506_END_STABILITY=6.0000
PRIMER_RIGHT_506_END_STABILITY=7.6000
PRIMER_PAIR_506_COMPL_ANY=4.00
PRIMER_PAIR_506_COMPL_END=1.00
PRIMER_PAIR_506_PRODUCT_SIZE=83
PRIMER_PAIR_506_PRODUCT_TM=71.4
PRIMER_PAIR_507_PENALTY=8.185582
PRIMER_LEFT_507_PENALTY=4.705093
PRIMER_RIGHT_507_PENALTY=3.480490
PRIMER_LEFT_507_SEQUENCE=TGGAGGTACCCACTGATGGTAAT
PRIMER_RIGHT_507_SEQUENCE=CATTCATGTGCATGTTCAGTC
PRIMER_LEFT_507=252,23
PRIMER_RIGHT_507=338,21
PRIMER_LEFT_507_TM=61.705
PRIMER_RIGHT_507_TM=57.520
PRIMER_LEFT_507_GC_PERCENT=47.826
PRIMER_RIGHT_507_GC_PERCENT=42.857
PRIMER_LEFT_507_SELF_ANY=6.00
PRIMER_RIGHT_507_SELF_ANY=6.00
PRIMER_LEFT_507_SELF_END=2.00
PRIMER_RIGHT_507_SELF_END=1.00
PRIMER_LEFT_507_END_STABILITY=5.6000
PRIMER_RIGHT_507_END_STABILITY=6.4000
PRIMER_PAIR_507_COMPL_ANY=5.00
PRIMER_PAIR_507_COMPL_END=1.00
PRIMER_PAIR_507_PRODUCT_SIZE=87
PRIMER_PAIR_507_PRODUCT_TM=74.7
PRIMER_PAIR_508_PENALTY=8.199312
PRIMER_LEFT_508_PENALTY=4.326727
PRIMER_RIGHT_508_PENALTY=3.872586
PRIMER_LEFT_508_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_508_SEQUENCE=CCAGTTCTGGATGGTCACTGG
PRIMER_LEFT_508=414,23
PRIMER_RIGHT_508=490,21
PRIMER_LEFT_508_TM=61.327
PRIMER_RIGHT_508_TM=62.873
PRIMER_LEFT_508_GC_PERCENT=43.478
PRIMER_RIGHT_508_GC_PERCENT=57.143
PRIMER_LEFT_508_SELF_ANY=5.00
PRIMER_RIGHT_508_SELF_ANY=6.00
PRIMER_LEFT_508_SELF_END=0.00
PRIMER_RIGHT_508_SELF_END=1.00
PRIMER_LEFT_508_END_STABILITY=7.0000
PRIMER_RIGHT_508_END_STABILITY=7.9000
PRIMER_PAIR_508_COMPL_ANY=4.00
PRIMER_PAIR_508_COMPL_END=3.00
PRIMER_PAIR_508_PRODUCT_SIZE=77
PRIMER_PAIR_508_PRODUCT_TM=72.3
PRIMER_PAIR_509_PENALTY=8.200452
PRIMER_LEFT_509_PENALTY=4.984244
PRIMER_RIGHT_509_PENALTY=3.216208
PRIMER_LEFT_509_SEQUENCE=GCTGGAGGTACCCACTGA
PRIMER_RIGHT_509_SEQUENCE=GTTCAGTCTGCCACAGAACAT
PRIMER_LEFT_509=250,18
PRIMER_RIGHT_509=325,21
PRIMER_LEFT_509_TM=57.016
PRIMER_RIGHT_509_TM=57.784
PRIMER_LEFT_509_GC_PERCENT=61.111
PRIMER_RIGHT_509_GC_PERCENT=47.619
PRIMER_LEFT_509_SELF_ANY=6.00
PRIMER_RIGHT_509_SELF_ANY=6.00
PRIMER_LEFT_509_SELF_END=2.00
PRIMER_RIGHT_509_SELF_END=2.00
PRIMER_LEFT_509_END_STABILITY=6.4000
PRIMER_RIGHT_509_END_STABILITY=6.6000
PRIMER_PAIR_509_COMPL_ANY=5.00
PRIMER_PAIR_509_COMPL_END=1.00
PRIMER_PAIR_509_PRODUCT_SIZE=76
PRIMER_PAIR_509_PRODUCT_TM=75.2
PRIMER_PAIR_510_PENALTY=8.227696
PRIMER_LEFT_510_PENALTY=2.227266
PRIMER_RIGHT_510_PENALTY=6.000430
PRIMER_LEFT_510_SEQUENCE=GTCCAGAATGGGAAGTGGGA
PRIMER_RIGHT_510_SEQUENCE=GTTCAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_510=338,20
PRIMER_RIGHT_510=437,24
PRIMER_LEFT_510_TM=62.227
PRIMER_RIGHT_510_TM=62.000
PRIMER_LEFT_510_GC_PERCENT=55.000
PRIMER_RIGHT_510_GC_PERCENT=45.833
PRIMER_LEFT_510_SELF_ANY=3.00
PRIMER_RIGHT_510_SELF_ANY=5.00
PRIMER_LEFT_510_SELF_END=0.00
PRIMER_RIGHT_510_SELF_END=3.00
PRIMER_LEFT_510_END_STABILITY=9.7000
PRIMER_RIGHT_510_END_STABILITY=8.4000
PRIMER_PAIR_510_COMPL_ANY=5.00
PRIMER_PAIR_510_COMPL_END=1.00
PRIMER_PAIR_510_PRODUCT_SIZE=100
PRIMER_PAIR_510_PRODUCT_TM=74.0
PRIMER_PAIR_511_PENALTY=8.239160
PRIMER_LEFT_511_PENALTY=4.089746
PRIMER_RIGHT_511_PENALTY=4.149413
PRIMER_LEFT_511_SEQUENCE=AGGGACCAAAACCTGCAT
PRIMER_RIGHT_511_SEQUENCE=TTGGTTGGCTTCTACCACATTG
PRIMER_LEFT_511=370,18
PRIMER_RIGHT_511=469,22
PRIMER_LEFT_511_TM=57.910
PRIMER_RIGHT_511_TM=62.149
PRIMER_LEFT_511_GC_PERCENT=50.000
PRIMER_RIGHT_511_GC_PERCENT=45.455
PRIMER_LEFT_511_SELF_ANY=4.00
PRIMER_RIGHT_511_SELF_ANY=4.00
PRIMER_LEFT_511_SELF_END=2.00
PRIMER_RIGHT_511_SELF_END=3.00
PRIMER_LEFT_511_END_STABILITY=8.4000
PRIMER_RIGHT_511_END_STABILITY=7.2000
PRIMER_PAIR_511_COMPL_ANY=5.00
PRIMER_PAIR_511_COMPL_END=1.00
PRIMER_PAIR_511_PRODUCT_SIZE=100
PRIMER_PAIR_511_PRODUCT_TM=73.6
PRIMER_PAIR_512_PENALTY=8.261706
PRIMER_LEFT_512_PENALTY=4.166335
PRIMER_RIGHT_512_PENALTY=4.095371
PRIMER_LEFT_512_SEQUENCE=GATTCCCTACCGCTGCTT
PRIMER_RIGHT_512_SEQUENCE=GTGTAAGAATTTGCACTTGTCAGG
PRIMER_LEFT_512=535,18
PRIMER_RIGHT_512=610,24
PRIMER_LEFT_512_TM=57.834
PRIMER_RIGHT_512_TM=60.095
PRIMER_LEFT_512_GC_PERCENT=55.556
PRIMER_RIGHT_512_GC_PERCENT=41.667
PRIMER_LEFT_512_SELF_ANY=3.00
PRIMER_RIGHT_512_SELF_ANY=5.00
PRIMER_LEFT_512_SELF_END=0.00
PRIMER_RIGHT_512_SELF_END=3.00
PRIMER_LEFT_512_END_STABILITY=8.5000
PRIMER_RIGHT_512_END_STABILITY=8.2000
PRIMER_PAIR_512_COMPL_ANY=4.00
PRIMER_PAIR_512_COMPL_END=1.00
PRIMER_PAIR_512_PRODUCT_SIZE=76
PRIMER_PAIR_512_PRODUCT_TM=70.9
PRIMER_PAIR_513_PENALTY=8.271289
PRIMER_LEFT_513_PENALTY=4.482644
PRIMER_RIGHT_513_PENALTY=3.788644
PRIMER_LEFT_513_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTAC
PRIMER_RIGHT_513_SEQUENCE=GATGGTCACTGGTTGGTTG
PRIMER_LEFT_513=407,24
PRIMER_RIGHT_513=481,19
PRIMER_LEFT_513_TM=59.517
PRIMER_RIGHT_513_TM=57.211
PRIMER_LEFT_513_GC_PERCENT=45.833
PRIMER_RIGHT_513_GC_PERCENT=52.632
PRIMER_LEFT_513_SELF_ANY=6.00
PRIMER_RIGHT_513_SELF_ANY=3.00
PRIMER_LEFT_513_SELF_END=3.00
PRIMER_RIGHT_513_SELF_END=0.00
PRIMER_LEFT_513_END_STABILITY=5.4000
PRIMER_RIGHT_513_END_STABILITY=8.2000
PRIMER_PAIR_513_COMPL_ANY=5.00
PRIMER_PAIR_513_COMPL_END=2.00
PRIMER_PAIR_513_PRODUCT_SIZE=75
PRIMER_PAIR_513_PRODUCT_TM=72.1
PRIMER_PAIR_514_PENALTY=8.297501
PRIMER_LEFT_514_PENALTY=4.198427
PRIMER_RIGHT_514_PENALTY=4.099075
PRIMER_LEFT_514_SEQUENCE=CGCTGCTTAGTTGGTGAGTTTG
PRIMER_RIGHT_514_SEQUENCE=ATCCTCTCCTGGTGTAAGAATTTG
PRIMER_LEFT_514=545,22
PRIMER_RIGHT_514=621,24
PRIMER_LEFT_514_TM=62.198
PRIMER_RIGHT_514_TM=59.901
PRIMER_LEFT_514_GC_PERCENT=50.000
PRIMER_RIGHT_514_GC_PERCENT=41.667
PRIMER_LEFT_514_SELF_ANY=3.00
PRIMER_RIGHT_514_SELF_ANY=4.00
PRIMER_LEFT_514_SELF_END=0.00
PRIMER_RIGHT_514_SELF_END=1.00
PRIMER_LEFT_514_END_STABILITY=7.0000
PRIMER_RIGHT_514_END_STABILITY=7.2000
PRIMER_PAIR_514_COMPL_ANY=4.00
PRIMER_PAIR_514_COMPL_END=0.00
PRIMER_PAIR_514_PRODUCT_SIZE=77
PRIMER_PAIR_514_PRODUCT_TM=71.3
PRIMER_PAIR_515_PENALTY=8.319889
PRIMER_LEFT_515_PENALTY=5.713033
PRIMER_RIGHT_515_PENALTY=2.606855
PRIMER_LEFT_515_SEQUENCE=GTACCCACTGATGGTAATGCTGG
PRIMER_RIGHT_515_SEQUENCE=CCCACTTCCCATTCTGGA
PRIMER_LEFT_515=257,23
PRIMER_RIGHT_515=356,18
PRIMER_LEFT_515_TM=62.713
PRIMER_RIGHT_515_TM=59.393
PRIMER_LEFT_515_GC_PERCENT=52.174
PRIMER_RIGHT_515_GC_PERCENT=55.556
PRIMER_LEFT_515_SELF_ANY=5.00
PRIMER_RIGHT_515_SELF_ANY=3.00
PRIMER_LEFT_515_SELF_END=0.00
PRIMER_RIGHT_515_SELF_END=2.00
PRIMER_LEFT_515_END_STABILITY=9.7000
PRIMER_RIGHT_515_END_STABILITY=8.2000
PRIMER_PAIR_515_COMPL_ANY=5.00
PRIMER_PAIR_515_COMPL_END=3.00
PRIMER_PAIR_515_PRODUCT_SIZE=100
PRIMER_PAIR_515_PRODUCT_TM=75.6
PRIMER_PAIR_516_PENALTY=8.321049
PRIMER_LEFT_516_PENALTY=4.840560
PRIMER_RIGHT_516_PENALTY=3.480490
PRIMER_LEFT_516_SEQUENCE=GAGGTACCCACTGATGGTAATGC
PRIMER_RIGHT_516_SEQUENCE=GACATTCATGTGCATGTTCAG
PRIMER_LEFT_516=254,23
PRIMER_RIGHT_516=340,21
PRIMER_LEFT_516_TM=61.841
PRIMER_RIGHT_516_TM=57.520
PRIMER_LEFT_516_GC_PERCENT=52.174
PRIMER_RIGHT_516_GC_PERCENT=42.857
PRIMER_LEFT_516_SELF_ANY=6.00
PRIMER_RIGHT_516_SELF_ANY=8.00
PRIMER_LEFT_516_SELF_END=2.00
PRIMER_RIGHT_516_SELF_END=2.00
PRIMER_LEFT_516_END_STABILITY=8.4000
PRIMER_RIGHT_516_END_STABILITY=7.0000
PRIMER_PAIR_516_COMPL_ANY=4.00
PRIMER_PAIR_516_COMPL_END=2.00
PRIMER_PAIR_516_PRODUCT_SIZE=87
PRIMER_PAIR_516_PRODUCT_TM=74.7
PRIMER_PAIR_517_PENALTY=8.327517
PRIMER_LEFT_517_PENALTY=3.747215
PRIMER_RIGHT_517_PENALTY=4.580302
PRIMER_LEFT_517_SEQUENCE=GTGAGTTTGTAAGTGATGCCCT
PRIMER_RIGHT_517_SEQUENCE=GTTTCGCAAACATCCATCCTCT
PRIMER_LEFT_517=558,22
PRIMER_RIGHT_517=636,22
PRIMER_LEFT_517_TM=58.253
PRIMER_RIGHT_517_TM=62.580
PRIMER_LEFT_517_GC_PERCENT=45.455
PRIMER_RIGHT_517_GC_PERCENT=45.455
PRIMER_LEFT_517_SELF_ANY=2.00
PRIMER_RIGHT_517_SELF_ANY=6.00
PRIMER_LEFT_517_SELF_END=2.00
PRIMER_RIGHT_517_SELF_END=0.00
PRIMER_LEFT_517_END_STABILITY=10.9000
PRIMER_RIGHT_517_END_STABILITY=7.9000
PRIMER_PAIR_517_COMPL_ANY=5.00
PRIMER_PAIR_517_COMPL_END=0.00
PRIMER_PAIR_517_PRODUCT_SIZE=79
PRIMER_PAIR_517_PRODUCT_TM=71.0
PRIMER_PAIR_518_PENALTY=8.341972
PRIMER_LEFT_518_PENALTY=5.168862
PRIMER_RIGHT_518_PENALTY=3.173109
PRIMER_LEFT_518_SEQUENCE=GTAGAAGCCAACCAACCAGTGAC
PRIMER_RIGHT_518_SEQUENCE=GCAGCGGTAGGGAATCACA
PRIMER_LEFT_518=455,23
PRIMER_RIGHT_518=550,19
PRIMER_LEFT_518_TM=62.169
PRIMER_RIGHT_518_TM=62.173
PRIMER_LEFT_518_GC_PERCENT=52.174
PRIMER_RIGHT_518_GC_PERCENT=57.895
PRIMER_LEFT_518_SELF_ANY=3.00
PRIMER_RIGHT_518_SELF_ANY=3.00
PRIMER_LEFT_518_SELF_END=3.00
PRIMER_RIGHT_518_SELF_END=1.00
PRIMER_LEFT_518_END_STABILITY=6.1000
PRIMER_RIGHT_518_END_STABILITY=6.7000
PRIMER_PAIR_518_COMPL_ANY=4.00
PRIMER_PAIR_518_COMPL_END=2.00
PRIMER_PAIR_518_PRODUCT_SIZE=96
PRIMER_PAIR_518_PRODUCT_TM=77.6
PRIMER_PAIR_519_PENALTY=8.349744
PRIMER_LEFT_519_PENALTY=4.610893
PRIMER_RIGHT_519_PENALTY=3.738851
PRIMER_LEFT_519_SEQUENCE=AGATCACCAATGTGGTAGAAGCC
PRIMER_RIGHT_519_SEQUENCE=CAAAGTGGGGATGGGTCT
PRIMER_LEFT_519=441,23
PRIMER_RIGHT_519=533,18
PRIMER_LEFT_519_TM=61.611
PRIMER_RIGHT_519_TM=58.261
PRIMER_LEFT_519_GC_PERCENT=47.826
PRIMER_RIGHT_519_GC_PERCENT=55.556
PRIMER_LEFT_519_SELF_ANY=6.00
PRIMER_RIGHT_519_SELF_ANY=2.00
PRIMER_LEFT_519_SELF_END=1.00
PRIMER_RIGHT_519_SELF_END=0.00
PRIMER_LEFT_519_END_STABILITY=9.7000
PRIMER_RIGHT_519_END_STABILITY=7.6000
PRIMER_PAIR_519_COMPL_ANY=6.00
PRIMER_PAIR_519_COMPL_END=2.00
PRIMER_PAIR_519_PRODUCT_SIZE=93
PRIMER_PAIR_519_PRODUCT_TM=76.4
PRIMER_PAIR_520_PENALTY=8.361511
PRIMER_LEFT_520_PENALTY=4.293610
PRIMER_RIGHT_520_PENALTY=4.067900
PRIMER_LEFT_520_SEQUENCE=GTGATTCCCTACCGCTGCTTAG
PRIMER_RIGHT_520_SEQUENCE=TAAGAATTTGCACTTGTCAGGAA
PRIMER_LEFT_520=533,22
PRIMER_RIGHT_520=607,23
PRIMER_LEFT_520_TM=62.294
PRIMER_RIGHT_520_TM=58.932
PRIMER_LEFT_520_GC_PERCENT=54.545
PRIMER_RIGHT_520_GC_PERCENT=34.783
PRIMER_LEFT_520_SELF_ANY=3.00
PRIMER_RIGHT_520_SELF_ANY=4.00
PRIMER_LEFT_520_SELF_END=3.00
PRIMER_RIGHT_520_SELF_END=0.00
PRIMER_LEFT_520_END_STABILITY=6.0000
PRIMER_RIGHT_520_END_STABILITY=8.2000
PRIMER_PAIR_520_COMPL_ANY=4.00
PRIMER_PAIR_520_COMPL_END=2.00
PRIMER_PAIR_520_PRODUCT_SIZE=75
PRIMER_PAIR_520_PRODUCT_TM=70.5
PRIMER_PAIR_521_PENALTY=8.388982
PRIMER_LEFT_521_PENALTY=4.293610
PRIMER_RIGHT_521_PENALTY=4.095371
PRIMER_LEFT_521_SEQUENCE=TGATTCCCTACCGCTGCTTAGT
PRIMER_RIGHT_521_SEQUENCE=GGTGTAAGAATTTGCACTTGTCAG
PRIMER_LEFT_521=534,22
PRIMER_RIGHT_521=611,24
PRIMER_LEFT_521_TM=62.294
PRIMER_RIGHT_521_TM=60.095
PRIMER_LEFT_521_GC_PERCENT=50.000
PRIMER_RIGHT_521_GC_PERCENT=41.667
PRIMER_LEFT_521_SELF_ANY=3.00
PRIMER_RIGHT_521_SELF_ANY=5.00
PRIMER_LEFT_521_SELF_END=1.00
PRIMER_RIGHT_521_SELF_END=1.00
PRIMER_LEFT_521_END_STABILITY=5.7000
PRIMER_RIGHT_521_END_STABILITY=6.4000
PRIMER_PAIR_521_COMPL_ANY=4.00
PRIMER_PAIR_521_COMPL_END=3.00
PRIMER_PAIR_521_PRODUCT_SIZE=78
PRIMER_PAIR_521_PRODUCT_TM=71.1
PRIMER_PAIR_522_PENALTY=8.394044
PRIMER_LEFT_522_PENALTY=3.813742
PRIMER_RIGHT_522_PENALTY=4.580302
PRIMER_LEFT_522_SEQUENCE=GTGAGTTTGTAAGTGATGCCC
PRIMER_RIGHT_522_SEQUENCE=AGTTTCGCAAACATCCATCCTC
PRIMER_LEFT_522=558,21
PRIMER_RIGHT_522=637,22
PRIMER_LEFT_522_TM=57.186
PRIMER_RIGHT_522_TM=62.580
PRIMER_LEFT_522_GC_PERCENT=47.619
PRIMER_RIGHT_522_GC_PERCENT=45.455
PRIMER_LEFT_522_SELF_ANY=2.00
PRIMER_RIGHT_522_SELF_ANY=6.00
PRIMER_LEFT_522_SELF_END=0.00
PRIMER_RIGHT_522_SELF_END=0.00
PRIMER_LEFT_522_END_STABILITY=11.2000
PRIMER_RIGHT_522_END_STABILITY=7.9000
PRIMER_PAIR_522_COMPL_ANY=5.00
PRIMER_PAIR_522_COMPL_END=2.00
PRIMER_PAIR_522_PRODUCT_SIZE=80
PRIMER_PAIR_522_PRODUCT_TM=70.9
PRIMER_PAIR_523_PENALTY=8.394044
PRIMER_LEFT_523_PENALTY=3.813742
PRIMER_RIGHT_523_PENALTY=4.580302
PRIMER_LEFT_523_SEQUENCE=GGTGAGTTTGTAAGTGATGCC
PRIMER_RIGHT_523_SEQUENCE=GAGTTTCGCAAACATCCATCCT
PRIMER_LEFT_523=557,21
PRIMER_RIGHT_523=638,22
PRIMER_LEFT_523_TM=57.186
PRIMER_RIGHT_523_TM=62.580
PRIMER_LEFT_523_GC_PERCENT=47.619
PRIMER_RIGHT_523_GC_PERCENT=45.455
PRIMER_LEFT_523_SELF_ANY=2.00
PRIMER_RIGHT_523_SELF_ANY=6.00
PRIMER_LEFT_523_SELF_END=1.00
PRIMER_RIGHT_523_SELF_END=0.00
PRIMER_LEFT_523_END_STABILITY=9.6000
PRIMER_RIGHT_523_END_STABILITY=7.8000
PRIMER_PAIR_523_COMPL_ANY=5.00
PRIMER_PAIR_523_COMPL_END=2.00
PRIMER_PAIR_523_PRODUCT_SIZE=82
PRIMER_PAIR_523_PRODUCT_TM=71.6
PRIMER_PAIR_524_PENALTY=8.397256
PRIMER_LEFT_524_PENALTY=3.597672
PRIMER_RIGHT_524_PENALTY=4.799584
PRIMER_LEFT_524_SEQUENCE=CCAGGAGAGGATGGATGTT
PRIMER_RIGHT_524_SEQUENCE=TGCAAGTTGGTACTCTTCTCACT
PRIMER_LEFT_524=610,19
PRIMER_RIGHT_524=696,23
PRIMER_LEFT_524_TM=57.402
PRIMER_RIGHT_524_TM=58.200
PRIMER_LEFT_524_GC_PERCENT=52.632
PRIMER_RIGHT_524_GC_PERCENT=43.478
PRIMER_LEFT_524_SELF_ANY=3.00
PRIMER_RIGHT_524_SELF_ANY=5.00
PRIMER_LEFT_524_SELF_END=0.00
PRIMER_RIGHT_524_SELF_END=1.00
PRIMER_LEFT_524_END_STABILITY=6.6000
PRIMER_RIGHT_524_END_STABILITY=6.4000
PRIMER_PAIR_524_COMPL_ANY=5.00
PRIMER_PAIR_524_COMPL_END=1.00
PRIMER_PAIR_524_PRODUCT_SIZE=87
PRIMER_PAIR_524_PRODUCT_TM=73.7
PRIMER_PAIR_525_PENALTY=8.402747
PRIMER_LEFT_525_PENALTY=2.395059
PRIMER_RIGHT_525_PENALTY=6.007688
PRIMER_LEFT_525_SEQUENCE=TCAGGGACCAAAACCTGC
PRIMER_RIGHT_525_SEQUENCE=ATCTGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_525=368,18
PRIMER_RIGHT_525=444,25
PRIMER_LEFT_525_TM=59.605
PRIMER_RIGHT_525_TM=61.008
PRIMER_LEFT_525_GC_PERCENT=55.556
PRIMER_RIGHT_525_GC_PERCENT=44.000
PRIMER_LEFT_525_SELF_ANY=4.00
PRIMER_RIGHT_525_SELF_ANY=6.00
PRIMER_LEFT_525_SELF_END=2.00
PRIMER_RIGHT_525_SELF_END=2.00
PRIMER_LEFT_525_END_STABILITY=9.7000
PRIMER_RIGHT_525_END_STABILITY=7.0000
PRIMER_PAIR_525_COMPL_ANY=4.00
PRIMER_PAIR_525_COMPL_END=1.00
PRIMER_PAIR_525_PRODUCT_SIZE=77
PRIMER_PAIR_525_PRODUCT_TM=71.8
PRIMER_PAIR_526_PENALTY=8.452406
PRIMER_LEFT_526_PENALTY=4.353331
PRIMER_RIGHT_526_PENALTY=4.099075
PRIMER_LEFT_526_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTA
PRIMER_RIGHT_526_SEQUENCE=CATCCTCTCCTGGTGTAAGAATTT
PRIMER_LEFT_526=546,23
PRIMER_RIGHT_526=622,24
PRIMER_LEFT_526_TM=58.647
PRIMER_RIGHT_526_TM=59.901
PRIMER_LEFT_526_GC_PERCENT=43.478
PRIMER_RIGHT_526_GC_PERCENT=41.667
PRIMER_LEFT_526_SELF_ANY=3.00
PRIMER_RIGHT_526_SELF_ANY=4.00
PRIMER_LEFT_526_SELF_END=2.00
PRIMER_RIGHT_526_SELF_END=3.00
PRIMER_LEFT_526_END_STABILITY=6.0000
PRIMER_RIGHT_526_END_STABILITY=7.2000
PRIMER_PAIR_526_COMPL_ANY=4.00
PRIMER_PAIR_526_COMPL_END=1.00
PRIMER_PAIR_526_PRODUCT_SIZE=77
PRIMER_PAIR_526_PRODUCT_TM=71.3
PRIMER_PAIR_527_PENALTY=8.474577
PRIMER_LEFT_527_PENALTY=4.697688
PRIMER_RIGHT_527_PENALTY=3.776889
PRIMER_LEFT_527_SEQUENCE=TCACCAATGTGGTAGAAGCCAA
PRIMER_RIGHT_527_SEQUENCE=GTCTTGCACTGCTTGCGG
PRIMER_LEFT_527=444,22
PRIMER_RIGHT_527=519,18
PRIMER_LEFT_527_TM=62.698
PRIMER_RIGHT_527_TM=61.777
PRIMER_LEFT_527_GC_PERCENT=45.455
PRIMER_RIGHT_527_GC_PERCENT=61.111
PRIMER_LEFT_527_SELF_ANY=6.00
PRIMER_RIGHT_527_SELF_ANY=5.00
PRIMER_LEFT_527_SELF_END=0.00
PRIMER_RIGHT_527_SELF_END=2.00
PRIMER_LEFT_527_END_STABILITY=10.0000
PRIMER_RIGHT_527_END_STABILITY=11.7000
PRIMER_PAIR_527_COMPL_ANY=4.00
PRIMER_PAIR_527_COMPL_END=2.00
PRIMER_PAIR_527_PRODUCT_SIZE=76
PRIMER_PAIR_527_PRODUCT_TM=75.2
PRIMER_PAIR_528_PENALTY=8.475039
PRIMER_LEFT_528_PENALTY=3.360461
PRIMER_RIGHT_528_PENALTY=5.114578
PRIMER_LEFT_528_SEQUENCE=CAAATTCTTACACCAGGAGAGGA
PRIMER_RIGHT_528_SEQUENCE=CTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_528=598,23
PRIMER_RIGHT_528=684,23
PRIMER_LEFT_528_TM=59.640
PRIMER_RIGHT_528_TM=57.885
PRIMER_LEFT_528_GC_PERCENT=43.478
PRIMER_RIGHT_528_GC_PERCENT=43.478
PRIMER_LEFT_528_SELF_ANY=4.00
PRIMER_RIGHT_528_SELF_ANY=4.00
PRIMER_LEFT_528_SELF_END=3.00
PRIMER_RIGHT_528_SELF_END=0.00
PRIMER_LEFT_528_END_STABILITY=7.9000
PRIMER_RIGHT_528_END_STABILITY=7.0000
PRIMER_PAIR_528_COMPL_ANY=6.00
PRIMER_PAIR_528_COMPL_END=2.00
PRIMER_PAIR_528_PRODUCT_SIZE=87
PRIMER_PAIR_528_PRODUCT_TM=72.8
PRIMER_PAIR_529_PENALTY=8.521362
PRIMER_LEFT_529_PENALTY=4.592761
PRIMER_RIGHT_529_PENALTY=3.928601
PRIMER_LEFT_529_SEQUENCE=ACTTTGTGATTCCCTACCGCTG
PRIMER_RIGHT_529_SEQUENCE=AATTTGCACTTGTCAGGAACGA
PRIMER_LEFT_529=528,22
PRIMER_RIGHT_529=603,22
PRIMER_LEFT_529_TM=62.593
PRIMER_RIGHT_529_TM=61.929
PRIMER_LEFT_529_GC_PERCENT=50.000
PRIMER_RIGHT_529_GC_PERCENT=40.909
PRIMER_LEFT_529_SELF_ANY=3.00
PRIMER_RIGHT_529_SELF_ANY=4.00
PRIMER_LEFT_529_SELF_END=2.00
PRIMER_RIGHT_529_SELF_END=1.00
PRIMER_LEFT_529_END_STABILITY=10.2000
PRIMER_RIGHT_529_END_STABILITY=8.4000
PRIMER_PAIR_529_COMPL_ANY=4.00
PRIMER_PAIR_529_COMPL_END=2.00
PRIMER_PAIR_529_PRODUCT_SIZE=76
PRIMER_PAIR_529_PRODUCT_TM=70.4
PRIMER_PAIR_530_PENALTY=8.521362
PRIMER_LEFT_530_PENALTY=4.592761
PRIMER_RIGHT_530_PENALTY=3.928601
PRIMER_LEFT_530_SEQUENCE=CACTTTGTGATTCCCTACCGCT
PRIMER_RIGHT_530_SEQUENCE=GAATTTGCACTTGTCAGGAACG
PRIMER_LEFT_530=527,22
PRIMER_RIGHT_530=604,22
PRIMER_LEFT_530_TM=62.593
PRIMER_RIGHT_530_TM=61.929
PRIMER_LEFT_530_GC_PERCENT=50.000
PRIMER_RIGHT_530_GC_PERCENT=45.455
PRIMER_LEFT_530_SELF_ANY=3.00
PRIMER_RIGHT_530_SELF_ANY=4.00
PRIMER_LEFT_530_SELF_END=2.00
PRIMER_RIGHT_530_SELF_END=2.00
PRIMER_LEFT_530_END_STABILITY=11.4000
PRIMER_RIGHT_530_END_STABILITY=8.4000
PRIMER_PAIR_530_COMPL_ANY=4.00
PRIMER_PAIR_530_COMPL_END=2.00
PRIMER_PAIR_530_PRODUCT_SIZE=78
PRIMER_PAIR_530_PRODUCT_TM=71.1
PRIMER_PAIR_531_PENALTY=8.543670
PRIMER_LEFT_531_PENALTY=4.411925
PRIMER_RIGHT_531_PENALTY=4.131746
PRIMER_LEFT_531_SEQUENCE=ACCGCTGCTTAGTTGGTGAGTT
PRIMER_RIGHT_531_SEQUENCE=CCTCTCCTGGTGTAAGAATTTG
PRIMER_LEFT_531=543,22
PRIMER_RIGHT_531=619,22
PRIMER_LEFT_531_TM=62.412
PRIMER_RIGHT_531_TM=57.868
PRIMER_LEFT_531_GC_PERCENT=50.000
PRIMER_RIGHT_531_GC_PERCENT=45.455
PRIMER_LEFT_531_SELF_ANY=3.00
PRIMER_RIGHT_531_SELF_ANY=4.00
PRIMER_LEFT_531_SELF_END=0.00
PRIMER_RIGHT_531_SELF_END=1.00
PRIMER_LEFT_531_END_STABILITY=6.4000
PRIMER_RIGHT_531_END_STABILITY=7.2000
PRIMER_PAIR_531_COMPL_ANY=4.00
PRIMER_PAIR_531_COMPL_END=0.00
PRIMER_PAIR_531_PRODUCT_SIZE=77
PRIMER_PAIR_531_PRODUCT_TM=71.8
PRIMER_PAIR_532_PENALTY=8.575486
PRIMER_LEFT_532_PENALTY=3.718176
PRIMER_RIGHT_532_PENALTY=4.857310
PRIMER_LEFT_532_SEQUENCE=ATTCTTACACCAGGAGAGGATGG
PRIMER_RIGHT_532_SEQUENCE=TGCAAGTTGGTACTCTTCTCAC
PRIMER_LEFT_532=601,23
PRIMER_RIGHT_532=696,22
PRIMER_LEFT_532_TM=60.718
PRIMER_RIGHT_532_TM=57.143
PRIMER_LEFT_532_GC_PERCENT=47.826
PRIMER_RIGHT_532_GC_PERCENT=45.455
PRIMER_LEFT_532_SELF_ANY=3.00
PRIMER_RIGHT_532_SELF_ANY=5.00
PRIMER_LEFT_532_SELF_END=0.00
PRIMER_RIGHT_532_SELF_END=0.00
PRIMER_LEFT_532_END_STABILITY=8.1000
PRIMER_RIGHT_532_END_STABILITY=6.4000
PRIMER_PAIR_532_COMPL_ANY=5.00
PRIMER_PAIR_532_COMPL_END=2.00
PRIMER_PAIR_532_PRODUCT_SIZE=96
PRIMER_PAIR_532_PRODUCT_TM=73.3
PRIMER_PAIR_533_PENALTY=8.613736
PRIMER_LEFT_533_PENALTY=3.380697
PRIMER_RIGHT_533_PENALTY=5.233039
PRIMER_LEFT_533_SEQUENCE=TTTGCGAAACTCATCTTCACT
PRIMER_RIGHT_533_SEQUENCE=AGTCATGCAAGTTGGTACTCTTCTC
PRIMER_LEFT_533=627,21
PRIMER_RIGHT_533=701,25
PRIMER_LEFT_533_TM=57.619
PRIMER_RIGHT_533_TM=60.233
PRIMER_LEFT_533_GC_PERCENT=38.095
PRIMER_RIGHT_533_GC_PERCENT=44.000
PRIMER_LEFT_533_SELF_ANY=4.00
PRIMER_RIGHT_533_SELF_ANY=5.00
PRIMER_LEFT_533_SELF_END=1.00
PRIMER_RIGHT_533_SELF_END=0.00
PRIMER_LEFT_533_END_STABILITY=6.4000
PRIMER_RIGHT_533_END_STABILITY=6.7000
PRIMER_PAIR_533_COMPL_ANY=4.00
PRIMER_PAIR_533_COMPL_END=1.00
PRIMER_PAIR_533_PRODUCT_SIZE=75
PRIMER_PAIR_533_PRODUCT_TM=71.6
PRIMER_PAIR_534_PENALTY=8.649974
PRIMER_LEFT_534_PENALTY=6.202271
PRIMER_RIGHT_534_PENALTY=2.447703
PRIMER_LEFT_534_SEQUENCE=AAAGAGACATGCAGTGAGAAGAGTA
PRIMER_RIGHT_534_SEQUENCE=AACACACAAACTCTACCCCTCG
PRIMER_LEFT_534=662,25
PRIMER_RIGHT_534=758,22
PRIMER_LEFT_534_TM=58.798
PRIMER_RIGHT_534_TM=60.448
PRIMER_LEFT_534_GC_PERCENT=40.000
PRIMER_RIGHT_534_GC_PERCENT=50.000
PRIMER_LEFT_534_SELF_ANY=4.00
PRIMER_RIGHT_534_SELF_ANY=2.00
PRIMER_LEFT_534_SELF_END=2.00
PRIMER_RIGHT_534_SELF_END=2.00
PRIMER_LEFT_534_END_STABILITY=5.4000
PRIMER_RIGHT_534_END_STABILITY=9.9000
PRIMER_PAIR_534_COMPL_ANY=5.00
PRIMER_PAIR_534_COMPL_END=3.00
PRIMER_PAIR_534_PRODUCT_SIZE=97
PRIMER_PAIR_534_PRODUCT_TM=73.6
PRIMER_PAIR_535_PENALTY=8.694212
PRIMER_LEFT_535_PENALTY=4.985387
PRIMER_RIGHT_535_PENALTY=3.708825
PRIMER_LEFT_535_SEQUENCE=GTGCAAGACCCATCCCCA
PRIMER_RIGHT_535_SEQUENCE=GTCAGGAACGAGAAGGGC
PRIMER_LEFT_535=511,18
PRIMER_RIGHT_535=592,18
PRIMER_LEFT_535_TM=62.985
PRIMER_RIGHT_535_TM=58.291
PRIMER_LEFT_535_GC_PERCENT=61.111
PRIMER_RIGHT_535_GC_PERCENT=61.111
PRIMER_LEFT_535_SELF_ANY=4.00
PRIMER_RIGHT_535_SELF_ANY=2.00
PRIMER_LEFT_535_SELF_END=0.00
PRIMER_RIGHT_535_SELF_END=2.00
PRIMER_LEFT_535_END_STABILITY=11.2000
PRIMER_RIGHT_535_END_STABILITY=10.9000
PRIMER_PAIR_535_COMPL_ANY=4.00
PRIMER_PAIR_535_COMPL_END=1.00
PRIMER_PAIR_535_PRODUCT_SIZE=82
PRIMER_PAIR_535_PRODUCT_TM=73.6
PRIMER_PAIR_536_PENALTY=8.696953
PRIMER_LEFT_536_PENALTY=4.792901
PRIMER_RIGHT_536_PENALTY=3.904052
PRIMER_LEFT_536_SEQUENCE=CAGATCACCAATGTGGTAGAAG
PRIMER_RIGHT_536_SEQUENCE=ACAAAGTGGGGATGGGTC
PRIMER_LEFT_536=440,22
PRIMER_RIGHT_536=534,18
PRIMER_LEFT_536_TM=57.207
PRIMER_RIGHT_536_TM=58.096
PRIMER_LEFT_536_GC_PERCENT=45.455
PRIMER_RIGHT_536_GC_PERCENT=55.556
PRIMER_LEFT_536_SELF_ANY=6.00
PRIMER_RIGHT_536_SELF_ANY=2.00
PRIMER_LEFT_536_SELF_END=2.00
PRIMER_RIGHT_536_SELF_END=1.00
PRIMER_LEFT_536_END_STABILITY=6.7000
PRIMER_RIGHT_536_END_STABILITY=9.1000
PRIMER_PAIR_536_COMPL_ANY=6.00
PRIMER_PAIR_536_COMPL_END=1.00
PRIMER_PAIR_536_PRODUCT_SIZE=95
PRIMER_PAIR_536_PRODUCT_TM=76.5
PRIMER_PAIR_537_PENALTY=8.711063
PRIMER_LEFT_537_PENALTY=4.187633
PRIMER_RIGHT_537_PENALTY=4.523430
PRIMER_LEFT_537_SEQUENCE=TTGGTGAGTTTGTAAGTGATGC
PRIMER_RIGHT_537_SEQUENCE=AAACATCCATCCTCTCCTGGTG
PRIMER_LEFT_537=555,22
PRIMER_RIGHT_537=629,22
PRIMER_LEFT_537_TM=57.812
PRIMER_RIGHT_537_TM=62.523
PRIMER_LEFT_537_GC_PERCENT=40.909
PRIMER_RIGHT_537_GC_PERCENT=50.000
PRIMER_LEFT_537_SELF_ANY=2.00
PRIMER_RIGHT_537_SELF_ANY=3.00
PRIMER_LEFT_537_SELF_END=2.00
PRIMER_RIGHT_537_SELF_END=3.00
PRIMER_LEFT_537_END_STABILITY=8.1000
PRIMER_RIGHT_537_END_STABILITY=8.2000
PRIMER_PAIR_537_COMPL_ANY=5.00
PRIMER_PAIR_537_COMPL_END=1.00
PRIMER_PAIR_537_PRODUCT_SIZE=75
PRIMER_PAIR_537_PRODUCT_TM=69.9
PRIMER_PAIR_538_PENALTY=8.723994
PRIMER_LEFT_538_PENALTY=3.536297
PRIMER_RIGHT_538_PENALTY=5.187697
PRIMER_LEFT_538_SEQUENCE=ATGTTTGCGAAACTCATCTTCAC
PRIMER_RIGHT_538_SEQUENCE=TCATGCAAGTTGGTACTCTTCTCA
PRIMER_LEFT_538=624,23
PRIMER_RIGHT_538=699,24
PRIMER_LEFT_538_TM=60.536
PRIMER_RIGHT_538_TM=61.188
PRIMER_LEFT_538_GC_PERCENT=39.130
PRIMER_RIGHT_538_GC_PERCENT=41.667
PRIMER_LEFT_538_SELF_ANY=6.00
PRIMER_RIGHT_538_SELF_ANY=5.00
PRIMER_LEFT_538_SELF_END=3.00
PRIMER_RIGHT_538_SELF_END=1.00
PRIMER_LEFT_538_END_STABILITY=6.7000
PRIMER_RIGHT_538_END_STABILITY=6.7000
PRIMER_PAIR_538_COMPL_ANY=4.00
PRIMER_PAIR_538_COMPL_END=2.00
PRIMER_PAIR_538_PRODUCT_SIZE=76
PRIMER_PAIR_538_PRODUCT_TM=71.4
PRIMER_PAIR_539_PENALTY=8.784018
PRIMER_LEFT_539_PENALTY=3.527826
PRIMER_RIGHT_539_PENALTY=5.256192
PRIMER_LEFT_539_SEQUENCE=GTGCAAATTCTTACACCAGGAGA
PRIMER_RIGHT_539_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_539=595,23
PRIMER_RIGHT_539=685,24
PRIMER_LEFT_539_TM=60.528
PRIMER_RIGHT_539_TM=58.744
PRIMER_LEFT_539_GC_PERCENT=43.478
PRIMER_RIGHT_539_GC_PERCENT=41.667
PRIMER_LEFT_539_SELF_ANY=5.00
PRIMER_RIGHT_539_SELF_ANY=4.00
PRIMER_LEFT_539_SELF_END=1.00
PRIMER_RIGHT_539_SELF_END=0.00
PRIMER_LEFT_539_END_STABILITY=7.9000
PRIMER_RIGHT_539_END_STABILITY=7.0000
PRIMER_PAIR_539_COMPL_ANY=5.00
PRIMER_PAIR_539_COMPL_END=3.00
PRIMER_PAIR_539_PRODUCT_SIZE=91
PRIMER_PAIR_539_PRODUCT_TM=73.1
PRIMER_PAIR_540_PENALTY=8.811095
PRIMER_LEFT_540_PENALTY=4.594300
PRIMER_RIGHT_540_PENALTY=4.216794
PRIMER_LEFT_540_SEQUENCE=ACCGCTGCTTAGTTGGTG
PRIMER_RIGHT_540_SEQUENCE=ATCCATCCTCTCCTGGTGTAAGA
PRIMER_LEFT_540=543,18
PRIMER_RIGHT_540=625,23
PRIMER_LEFT_540_TM=57.406
PRIMER_RIGHT_540_TM=61.217
PRIMER_LEFT_540_GC_PERCENT=55.556
PRIMER_RIGHT_540_GC_PERCENT=47.826
PRIMER_LEFT_540_SELF_ANY=3.00
PRIMER_RIGHT_540_SELF_ANY=3.00
PRIMER_LEFT_540_SELF_END=0.00
PRIMER_RIGHT_540_SELF_END=3.00
PRIMER_LEFT_540_END_STABILITY=8.2000
PRIMER_RIGHT_540_END_STABILITY=6.0000
PRIMER_PAIR_540_COMPL_ANY=4.00
PRIMER_PAIR_540_COMPL_END=0.00
PRIMER_PAIR_540_PRODUCT_SIZE=83
PRIMER_PAIR_540_PRODUCT_TM=71.9
PRIMER_PAIR_541_PENALTY=8.816618
PRIMER_LEFT_541_PENALTY=4.293188
PRIMER_RIGHT_541_PENALTY=4.523430
PRIMER_LEFT_541_SEQUENCE=TTGGTGAGTTTGTAAGTGATGCC
PRIMER_RIGHT_541_SEQUENCE=CAAACATCCATCCTCTCCTGGT
PRIMER_LEFT_541=555,23
PRIMER_RIGHT_541=630,22
PRIMER_LEFT_541_TM=61.293
PRIMER_RIGHT_541_TM=62.523
PRIMER_LEFT_541_GC_PERCENT=43.478
PRIMER_RIGHT_541_GC_PERCENT=50.000
PRIMER_LEFT_541_SELF_ANY=2.00
PRIMER_RIGHT_541_SELF_ANY=3.00
PRIMER_LEFT_541_SELF_END=1.00
PRIMER_RIGHT_541_SELF_END=1.00
PRIMER_LEFT_541_END_STABILITY=9.6000
PRIMER_RIGHT_541_END_STABILITY=7.9000
PRIMER_PAIR_541_COMPL_ANY=5.00
PRIMER_PAIR_541_COMPL_END=1.00
PRIMER_PAIR_541_PRODUCT_SIZE=76
PRIMER_PAIR_541_PRODUCT_TM=70.4
PRIMER_PAIR_542_PENALTY=8.836638
PRIMER_LEFT_542_PENALTY=3.968084
PRIMER_RIGHT_542_PENALTY=4.868554
PRIMER_LEFT_542_SEQUENCE=GAGGATGGATGTTTGCGA
PRIMER_RIGHT_542_SEQUENCE=TGCAAGTTGGTACTCTTCTCACTG
PRIMER_LEFT_542=616,18
PRIMER_RIGHT_542=696,24
PRIMER_LEFT_542_TM=58.032
PRIMER_RIGHT_542_TM=60.869
PRIMER_LEFT_542_GC_PERCENT=50.000
PRIMER_RIGHT_542_GC_PERCENT=45.833
PRIMER_LEFT_542_SELF_ANY=2.00
PRIMER_RIGHT_542_SELF_ANY=5.00
PRIMER_LEFT_542_SELF_END=2.00
PRIMER_RIGHT_542_SELF_END=3.00
PRIMER_LEFT_542_END_STABILITY=10.2000
PRIMER_RIGHT_542_END_STABILITY=6.7000
PRIMER_PAIR_542_COMPL_ANY=4.00
PRIMER_PAIR_542_COMPL_END=0.00
PRIMER_PAIR_542_PRODUCT_SIZE=81
PRIMER_PAIR_542_PRODUCT_TM=72.7
PRIMER_PAIR_543_PENALTY=8.852178
PRIMER_LEFT_543_PENALTY=5.351477
PRIMER_RIGHT_543_PENALTY=3.500701
PRIMER_LEFT_543_SEQUENCE=CTGGAGGTACCCACTGATGGTAA
PRIMER_RIGHT_543_SEQUENCE=ACATTCATGTGCATGTTCAGTC
PRIMER_LEFT_543=251,23
PRIMER_RIGHT_543=339,22
PRIMER_LEFT_543_TM=62.351
PRIMER_RIGHT_543_TM=58.499
PRIMER_LEFT_543_GC_PERCENT=52.174
PRIMER_RIGHT_543_GC_PERCENT=40.909
PRIMER_LEFT_543_SELF_ANY=6.00
PRIMER_RIGHT_543_SELF_ANY=8.00
PRIMER_LEFT_543_SELF_END=3.00
PRIMER_RIGHT_543_SELF_END=1.00
PRIMER_LEFT_543_END_STABILITY=7.2000
PRIMER_RIGHT_543_END_STABILITY=6.4000
PRIMER_PAIR_543_COMPL_ANY=5.00
PRIMER_PAIR_543_COMPL_END=1.00
PRIMER_PAIR_543_PRODUCT_SIZE=89
PRIMER_PAIR_543_PRODUCT_TM=74.8
PRIMER_PAIR_544_PENALTY=8.929890
PRIMER_LEFT_544_PENALTY=3.487465
PRIMER_RIGHT_544_PENALTY=5.442425
PRIMER_LEFT_544_SEQUENCE=GTTTGCGAAACTCATCTTCACT
PRIMER_RIGHT_544_SEQUENCE=GTCATGCAAGTTGGTACTCTTCT
PRIMER_LEFT_544=626,22
PRIMER_RIGHT_544=700,23
PRIMER_LEFT_544_TM=58.513
PRIMER_RIGHT_544_TM=57.558
PRIMER_LEFT_544_GC_PERCENT=40.909
PRIMER_RIGHT_544_GC_PERCENT=43.478
PRIMER_LEFT_544_SELF_ANY=6.00
PRIMER_RIGHT_544_SELF_ANY=5.00
PRIMER_LEFT_544_SELF_END=1.00
PRIMER_RIGHT_544_SELF_END=2.00
PRIMER_LEFT_544_END_STABILITY=6.4000
PRIMER_RIGHT_544_END_STABILITY=6.7000
PRIMER_PAIR_544_COMPL_ANY=4.00
PRIMER_PAIR_544_COMPL_END=0.00
PRIMER_PAIR_544_PRODUCT_SIZE=75
PRIMER_PAIR_544_PRODUCT_TM=72.1
PRIMER_PAIR_545_PENALTY=8.956596
PRIMER_LEFT_545_PENALTY=4.590174
PRIMER_RIGHT_545_PENALTY=4.366422
PRIMER_LEFT_545_SEQUENCE=GGACCAAAACCTGCATTG
PRIMER_RIGHT_545_SEQUENCE=GGTTGGTTGGCTTCTACCACAT
PRIMER_LEFT_545=372,18
PRIMER_RIGHT_545=471,22
PRIMER_LEFT_545_TM=57.410
PRIMER_RIGHT_545_TM=62.366
PRIMER_LEFT_545_GC_PERCENT=50.000
PRIMER_RIGHT_545_GC_PERCENT=50.000
PRIMER_LEFT_545_SELF_ANY=4.00
PRIMER_RIGHT_545_SELF_ANY=4.00
PRIMER_LEFT_545_SELF_END=3.00
PRIMER_RIGHT_545_SELF_END=2.00
PRIMER_LEFT_545_END_STABILITY=7.2000
PRIMER_RIGHT_545_END_STABILITY=6.6000
PRIMER_PAIR_545_COMPL_ANY=5.00
PRIMER_PAIR_545_COMPL_END=2.00
PRIMER_PAIR_545_PRODUCT_SIZE=100
PRIMER_PAIR_545_PRODUCT_TM=74.0
PRIMER_PAIR_546_PENALTY=8.977719
PRIMER_LEFT_546_PENALTY=3.535294
PRIMER_RIGHT_546_PENALTY=5.442425
PRIMER_LEFT_546_SEQUENCE=GTTTGCGAAACTCATCTTCAC
PRIMER_RIGHT_546_SEQUENCE=AGTCATGCAAGTTGGTACTCTTC
PRIMER_LEFT_546=626,21
PRIMER_RIGHT_546=701,23
PRIMER_LEFT_546_TM=57.465
PRIMER_RIGHT_546_TM=57.558
PRIMER_LEFT_546_GC_PERCENT=42.857
PRIMER_RIGHT_546_GC_PERCENT=43.478
PRIMER_LEFT_546_SELF_ANY=6.00
PRIMER_RIGHT_546_SELF_ANY=5.00
PRIMER_LEFT_546_SELF_END=3.00
PRIMER_RIGHT_546_SELF_END=0.00
PRIMER_LEFT_546_END_STABILITY=6.7000
PRIMER_RIGHT_546_END_STABILITY=6.7000
PRIMER_PAIR_546_COMPL_ANY=4.00
PRIMER_PAIR_546_COMPL_END=1.00
PRIMER_PAIR_546_PRODUCT_SIZE=76
PRIMER_PAIR_546_PRODUCT_TM=72.0
PRIMER_PAIR_547_PENALTY=8.992907
PRIMER_LEFT_547_PENALTY=5.831817
PRIMER_RIGHT_547_PENALTY=3.161089
PRIMER_LEFT_547_SEQUENCE=TCCTGCAGTATTGCCAAGAAGTC
PRIMER_RIGHT_547_SEQUENCE=TGGTCACTGGTTGGTTGG
PRIMER_LEFT_547=405,23
PRIMER_RIGHT_547=479,18
PRIMER_LEFT_547_TM=62.832
PRIMER_RIGHT_547_TM=58.839
PRIMER_LEFT_547_GC_PERCENT=47.826
PRIMER_RIGHT_547_GC_PERCENT=55.556
PRIMER_LEFT_547_SELF_ANY=6.00
PRIMER_RIGHT_547_SELF_ANY=3.00
PRIMER_LEFT_547_SELF_END=2.00
PRIMER_RIGHT_547_SELF_END=0.00
PRIMER_LEFT_547_END_STABILITY=6.4000
PRIMER_RIGHT_547_END_STABILITY=8.2000
PRIMER_PAIR_547_COMPL_ANY=5.00
PRIMER_PAIR_547_COMPL_END=2.00
PRIMER_PAIR_547_PRODUCT_SIZE=75
PRIMER_PAIR_547_PRODUCT_TM=72.1
PRIMER_PAIR_548_PENALTY=9.029769
PRIMER_LEFT_548_PENALTY=3.647215
PRIMER_RIGHT_548_PENALTY=5.382554
PRIMER_LEFT_548_SEQUENCE=TGCAAATTCTTACACCAGGAGAG
PRIMER_RIGHT_548_SEQUENCE=GGTACTCTTCTCACTGCATGTCT
PRIMER_LEFT_548=596,23
PRIMER_RIGHT_548=688,23
PRIMER_LEFT_548_TM=60.647
PRIMER_RIGHT_548_TM=57.617
PRIMER_LEFT_548_GC_PERCENT=43.478
PRIMER_RIGHT_548_GC_PERCENT=47.826
PRIMER_LEFT_548_SELF_ANY=4.00
PRIMER_RIGHT_548_SELF_ANY=4.00
PRIMER_LEFT_548_SELF_END=0.00
PRIMER_RIGHT_548_SELF_END=0.00
PRIMER_LEFT_548_END_STABILITY=6.4000
PRIMER_RIGHT_548_END_STABILITY=6.4000
PRIMER_PAIR_548_COMPL_ANY=5.00
PRIMER_PAIR_548_COMPL_END=2.00
PRIMER_PAIR_548_PRODUCT_SIZE=93
PRIMER_PAIR_548_PRODUCT_TM=73.3
PRIMER_PAIR_549_PENALTY=9.051676
PRIMER_LEFT_549_PENALTY=3.686405
PRIMER_RIGHT_549_PENALTY=5.365271
PRIMER_LEFT_549_SEQUENCE=AAGTGCAAATTCTTACACCAGG
PRIMER_RIGHT_549_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCT
PRIMER_LEFT_549=593,22
PRIMER_RIGHT_549=688,25
PRIMER_LEFT_549_TM=58.314
PRIMER_RIGHT_549_TM=60.365
PRIMER_LEFT_549_GC_PERCENT=40.909
PRIMER_RIGHT_549_GC_PERCENT=48.000
PRIMER_LEFT_549_SELF_ANY=5.00
PRIMER_RIGHT_549_SELF_ANY=4.00
PRIMER_LEFT_549_SELF_END=3.00
PRIMER_RIGHT_549_SELF_END=0.00
PRIMER_LEFT_549_END_STABILITY=9.7000
PRIMER_RIGHT_549_END_STABILITY=6.4000
PRIMER_PAIR_549_COMPL_ANY=4.00
PRIMER_PAIR_549_COMPL_END=1.00
PRIMER_PAIR_549_PRODUCT_SIZE=96
PRIMER_PAIR_549_PRODUCT_TM=73.3
PRIMER_PAIR_550_PENALTY=9.061120
PRIMER_LEFT_550_PENALTY=6.336480
PRIMER_RIGHT_550_PENALTY=2.724641
PRIMER_LEFT_550_SEQUENCE=CAAAGAGACATGCAGTGAGAAGAGT
PRIMER_RIGHT_550_SEQUENCE=AAACTCTACCCCTCGGAACTT
PRIMER_LEFT_550=661,25
PRIMER_RIGHT_550=751,21
PRIMER_LEFT_550_TM=61.336
PRIMER_RIGHT_550_TM=58.275
PRIMER_LEFT_550_GC_PERCENT=44.000
PRIMER_RIGHT_550_GC_PERCENT=47.619
PRIMER_LEFT_550_SELF_ANY=4.00
PRIMER_RIGHT_550_SELF_ANY=3.00
PRIMER_LEFT_550_SELF_END=1.00
PRIMER_RIGHT_550_SELF_END=3.00
PRIMER_LEFT_550_END_STABILITY=6.1000
PRIMER_RIGHT_550_END_STABILITY=6.7000
PRIMER_PAIR_550_COMPL_ANY=5.00
PRIMER_PAIR_550_COMPL_END=2.00
PRIMER_PAIR_550_PRODUCT_SIZE=91
PRIMER_PAIR_550_PRODUCT_TM=73.6
PRIMER_PAIR_551_PENALTY=9.062113
PRIMER_LEFT_551_PENALTY=2.423358
PRIMER_RIGHT_551_PENALTY=6.638755
PRIMER_LEFT_551_SEQUENCE=TCCAGAATGGGAAGTGGGA
PRIMER_RIGHT_551_SEQUENCE=TCAGGGTAGACTTCTTGGCAATACT
PRIMER_LEFT_551=339,19
PRIMER_RIGHT_551=435,25
PRIMER_LEFT_551_TM=61.423
PRIMER_RIGHT_551_TM=61.639
PRIMER_LEFT_551_GC_PERCENT=52.632
PRIMER_RIGHT_551_GC_PERCENT=44.000
PRIMER_LEFT_551_SELF_ANY=3.00
PRIMER_RIGHT_551_SELF_ANY=5.00
PRIMER_LEFT_551_SELF_END=0.00
PRIMER_RIGHT_551_SELF_END=2.00
PRIMER_LEFT_551_END_STABILITY=9.7000
PRIMER_RIGHT_551_END_STABILITY=5.3000
PRIMER_PAIR_551_COMPL_ANY=5.00
PRIMER_PAIR_551_COMPL_END=1.00
PRIMER_PAIR_551_PRODUCT_SIZE=97
PRIMER_PAIR_551_PRODUCT_TM=73.6
PRIMER_PAIR_552_PENALTY=9.078622
PRIMER_LEFT_552_PENALTY=4.983250
PRIMER_RIGHT_552_PENALTY=4.095371
PRIMER_LEFT_552_SEQUENCE=TTTGTGATTCCCTACCGC
PRIMER_RIGHT_552_SEQUENCE=CTGGTGTAAGAATTTGCACTTGTC
PRIMER_LEFT_552=530,18
PRIMER_RIGHT_552=613,24
PRIMER_LEFT_552_TM=57.017
PRIMER_RIGHT_552_TM=60.095
PRIMER_LEFT_552_GC_PERCENT=50.000
PRIMER_RIGHT_552_GC_PERCENT=41.667
PRIMER_LEFT_552_SELF_ANY=3.00
PRIMER_RIGHT_552_SELF_ANY=5.00
PRIMER_LEFT_552_SELF_END=2.00
PRIMER_RIGHT_552_SELF_END=2.00
PRIMER_LEFT_552_END_STABILITY=11.1000
PRIMER_RIGHT_552_END_STABILITY=6.7000
PRIMER_PAIR_552_COMPL_ANY=4.00
PRIMER_PAIR_552_COMPL_END=0.00
PRIMER_PAIR_552_PRODUCT_SIZE=84
PRIMER_PAIR_552_PRODUCT_TM=71.3
PRIMER_PAIR_553_PENALTY=9.144420
PRIMER_LEFT_553_PENALTY=5.925828
PRIMER_RIGHT_553_PENALTY=3.218591
PRIMER_LEFT_553_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTA
PRIMER_RIGHT_553_SEQUENCE=TGGATGGTCACTGGTTGG
PRIMER_LEFT_553=406,24
PRIMER_RIGHT_553=483,18
PRIMER_LEFT_553_TM=61.926
PRIMER_RIGHT_553_TM=58.781
PRIMER_LEFT_553_GC_PERCENT=45.833
PRIMER_RIGHT_553_GC_PERCENT=55.556
PRIMER_LEFT_553_SELF_ANY=6.00
PRIMER_RIGHT_553_SELF_ANY=3.00
PRIMER_LEFT_553_SELF_END=2.00
PRIMER_RIGHT_553_SELF_END=0.00
PRIMER_LEFT_553_END_STABILITY=5.4000
PRIMER_RIGHT_553_END_STABILITY=8.2000
PRIMER_PAIR_553_COMPL_ANY=5.00
PRIMER_PAIR_553_COMPL_END=3.00
PRIMER_PAIR_553_PRODUCT_SIZE=78
PRIMER_PAIR_553_PRODUCT_TM=72.7
PRIMER_PAIR_554_PENALTY=9.168577
PRIMER_LEFT_554_PENALTY=4.841851
PRIMER_RIGHT_554_PENALTY=4.326727
PRIMER_LEFT_554_SEQUENCE=CCGCTGCTTAGTTGGTGAGTTT
PRIMER_RIGHT_554_SEQUENCE=CCTCTCCTGGTGTAAGAATTTGC
PRIMER_LEFT_554=544,22
PRIMER_RIGHT_554=619,23
PRIMER_LEFT_554_TM=62.842
PRIMER_RIGHT_554_TM=61.327
PRIMER_LEFT_554_GC_PERCENT=50.000
PRIMER_RIGHT_554_GC_PERCENT=47.826
PRIMER_LEFT_554_SELF_ANY=3.00
PRIMER_RIGHT_554_SELF_ANY=4.00
PRIMER_LEFT_554_SELF_END=0.00
PRIMER_RIGHT_554_SELF_END=2.00
PRIMER_LEFT_554_END_STABILITY=6.7000
PRIMER_RIGHT_554_END_STABILITY=8.8000
PRIMER_PAIR_554_COMPL_ANY=4.00
PRIMER_PAIR_554_COMPL_END=0.00
PRIMER_PAIR_554_PRODUCT_SIZE=76
PRIMER_PAIR_554_PRODUCT_TM=72.0
PRIMER_PAIR_555_PENALTY=9.177766
PRIMER_LEFT_555_PENALTY=3.234765
PRIMER_RIGHT_555_PENALTY=5.943001
PRIMER_LEFT_555_SEQUENCE=GCGAAACTCATCTTCACTGGC
PRIMER_RIGHT_555_SEQUENCE=CGTAGTCATGCAAGTTGGTACTCTT
PRIMER_LEFT_555=630,21
PRIMER_RIGHT_555=704,25
PRIMER_LEFT_555_TM=62.235
PRIMER_RIGHT_555_TM=60.943
PRIMER_LEFT_555_GC_PERCENT=52.381
PRIMER_RIGHT_555_GC_PERCENT=44.000
PRIMER_LEFT_555_SELF_ANY=3.00
PRIMER_RIGHT_555_SELF_ANY=5.00
PRIMER_LEFT_555_SELF_END=2.00
PRIMER_RIGHT_555_SELF_END=0.00
PRIMER_LEFT_555_END_STABILITY=9.7000
PRIMER_RIGHT_555_END_STABILITY=6.7000
PRIMER_PAIR_555_COMPL_ANY=4.00
PRIMER_PAIR_555_COMPL_END=2.00
PRIMER_PAIR_555_PRODUCT_SIZE=75
PRIMER_PAIR_555_PRODUCT_TM=72.7
PRIMER_PAIR_556_PENALTY=9.184810
PRIMER_LEFT_556_PENALTY=3.532432
PRIMER_RIGHT_556_PENALTY=5.652377
PRIMER_LEFT_556_SEQUENCE=TCCTGACAAGTGCAAATTCTT
PRIMER_RIGHT_556_SEQUENCE=ACTCTTCTCACTGCATGTCTCTT
PRIMER_LEFT_556=586,21
PRIMER_RIGHT_556=685,23
PRIMER_LEFT_556_TM=57.468
PRIMER_RIGHT_556_TM=57.348
PRIMER_LEFT_556_GC_PERCENT=38.095
PRIMER_RIGHT_556_GC_PERCENT=43.478
PRIMER_LEFT_556_SELF_ANY=4.00
PRIMER_RIGHT_556_SELF_ANY=4.00
PRIMER_LEFT_556_SELF_END=1.00
PRIMER_RIGHT_556_SELF_END=0.00
PRIMER_LEFT_556_END_STABILITY=7.0000
PRIMER_RIGHT_556_END_STABILITY=6.7000
PRIMER_PAIR_556_COMPL_ANY=5.00
PRIMER_PAIR_556_COMPL_END=1.00
PRIMER_PAIR_556_PRODUCT_SIZE=100
PRIMER_PAIR_556_PRODUCT_TM=73.6
PRIMER_PAIR_557_PENALTY=9.213735
PRIMER_LEFT_557_PENALTY=5.713033
PRIMER_RIGHT_557_PENALTY=3.500701
PRIMER_LEFT_557_SEQUENCE=GGTACCCACTGATGGTAATGCTG
PRIMER_RIGHT_557_SEQUENCE=GACATTCATGTGCATGTTCAGT
PRIMER_LEFT_557=256,23
PRIMER_RIGHT_557=340,22
PRIMER_LEFT_557_TM=62.713
PRIMER_RIGHT_557_TM=58.499
PRIMER_LEFT_557_GC_PERCENT=52.174
PRIMER_RIGHT_557_GC_PERCENT=40.909
PRIMER_LEFT_557_SELF_ANY=6.00
PRIMER_RIGHT_557_SELF_ANY=8.00
PRIMER_LEFT_557_SELF_END=1.00
PRIMER_RIGHT_557_SELF_END=2.00
PRIMER_LEFT_557_END_STABILITY=8.5000
PRIMER_RIGHT_557_END_STABILITY=6.4000
PRIMER_PAIR_557_COMPL_ANY=5.00
PRIMER_PAIR_557_COMPL_END=2.00
PRIMER_PAIR_557_PRODUCT_SIZE=85
PRIMER_PAIR_557_PRODUCT_TM=74.5
PRIMER_PAIR_558_PENALTY=9.236373
PRIMER_LEFT_558_PENALTY=6.939511
PRIMER_RIGHT_558_PENALTY=2.296862
PRIMER_LEFT_558_SEQUENCE=ATCCTGCAGTATTGCCAAGAAGTC
PRIMER_RIGHT_558_SEQUENCE=GGTCACTGGTTGGTTGGCTT
PRIMER_LEFT_558=404,24
PRIMER_RIGHT_558=478,20
PRIMER_LEFT_558_TM=62.940
PRIMER_RIGHT_558_TM=62.297
PRIMER_LEFT_558_GC_PERCENT=45.833
PRIMER_RIGHT_558_GC_PERCENT=55.000
PRIMER_LEFT_558_SELF_ANY=8.00
PRIMER_RIGHT_558_SELF_ANY=3.00
PRIMER_LEFT_558_SELF_END=2.00
PRIMER_RIGHT_558_SELF_END=0.00
PRIMER_LEFT_558_END_STABILITY=6.4000
PRIMER_RIGHT_558_END_STABILITY=9.7000
PRIMER_PAIR_558_COMPL_ANY=5.00
PRIMER_PAIR_558_COMPL_END=3.00
PRIMER_PAIR_558_PRODUCT_SIZE=75
PRIMER_PAIR_558_PRODUCT_TM=72.1
PRIMER_PAIR_559_PENALTY=9.244053
PRIMER_LEFT_559_PENALTY=4.310125
PRIMER_RIGHT_559_PENALTY=4.933928
PRIMER_LEFT_559_SEQUENCE=GTTGGTGAGTTTGTAAGTGATGC
PRIMER_RIGHT_559_SEQUENCE=CGCAAACATCCATCCTCT
PRIMER_LEFT_559=554,23
PRIMER_RIGHT_559=632,18
PRIMER_LEFT_559_TM=58.690
PRIMER_RIGHT_559_TM=57.066
PRIMER_LEFT_559_GC_PERCENT=43.478
PRIMER_RIGHT_559_GC_PERCENT=50.000
PRIMER_LEFT_559_SELF_ANY=2.00
PRIMER_RIGHT_559_SELF_ANY=2.00
PRIMER_LEFT_559_SELF_END=2.00
PRIMER_RIGHT_559_SELF_END=0.00
PRIMER_LEFT_559_END_STABILITY=8.1000
PRIMER_RIGHT_559_END_STABILITY=7.9000
PRIMER_PAIR_559_COMPL_ANY=5.00
PRIMER_PAIR_559_COMPL_END=3.00
PRIMER_PAIR_559_PRODUCT_SIZE=79
PRIMER_PAIR_559_PRODUCT_TM=71.5
PRIMER_PAIR_560_PENALTY=9.287367
PRIMER_LEFT_560_PENALTY=3.579454
PRIMER_RIGHT_560_PENALTY=5.707913
PRIMER_LEFT_560_SEQUENCE=CTCGTTCCTGACAAGTGCAAAT
PRIMER_RIGHT_560_SEQUENCE=GGTGTGCCAGTGAAGATGAGTTT
PRIMER_LEFT_560=581,22
PRIMER_RIGHT_560=655,23
PRIMER_LEFT_560_TM=61.579
PRIMER_RIGHT_560_TM=62.708
PRIMER_LEFT_560_GC_PERCENT=45.455
PRIMER_RIGHT_560_GC_PERCENT=47.826
PRIMER_LEFT_560_SELF_ANY=4.00
PRIMER_RIGHT_560_SELF_ANY=3.00
PRIMER_LEFT_560_SELF_END=2.00
PRIMER_RIGHT_560_SELF_END=1.00
PRIMER_LEFT_560_END_STABILITY=7.2000
PRIMER_RIGHT_560_END_STABILITY=6.7000
PRIMER_PAIR_560_COMPL_ANY=5.00
PRIMER_PAIR_560_COMPL_END=2.00
PRIMER_PAIR_560_PRODUCT_SIZE=75
PRIMER_PAIR_560_PRODUCT_TM=71.6
PRIMER_PAIR_561_PENALTY=9.365653
PRIMER_LEFT_561_PENALTY=4.370754
PRIMER_RIGHT_561_PENALTY=4.994899
PRIMER_LEFT_561_SEQUENCE=AGTTGGTGAGTTTGTAAGTGATGC
PRIMER_RIGHT_561_SEQUENCE=ACATCCATCCTCTCCTGGTGTAA
PRIMER_LEFT_561=553,24
PRIMER_RIGHT_561=627,23
PRIMER_LEFT_561_TM=59.629
PRIMER_RIGHT_561_TM=61.995
PRIMER_LEFT_561_GC_PERCENT=41.667
PRIMER_RIGHT_561_GC_PERCENT=47.826
PRIMER_LEFT_561_SELF_ANY=2.00
PRIMER_RIGHT_561_SELF_ANY=4.00
PRIMER_LEFT_561_SELF_END=2.00
PRIMER_RIGHT_561_SELF_END=1.00
PRIMER_LEFT_561_END_STABILITY=8.1000
PRIMER_RIGHT_561_END_STABILITY=6.0000
PRIMER_PAIR_561_COMPL_ANY=5.00
PRIMER_PAIR_561_COMPL_END=0.00
PRIMER_PAIR_561_PRODUCT_SIZE=75
PRIMER_PAIR_561_PRODUCT_TM=70.5
PRIMER_PAIR_562_PENALTY=9.446797
PRIMER_LEFT_562_PENALTY=3.991211
PRIMER_RIGHT_562_PENALTY=5.455586
PRIMER_LEFT_562_SEQUENCE=GCAAATTCTTACACCAGGAGAG
PRIMER_RIGHT_562_SEQUENCE=AAGTTGGTACTCTTCTCACTGCAT
PRIMER_LEFT_562=597,22
PRIMER_RIGHT_562=693,24
PRIMER_LEFT_562_TM=58.009
PRIMER_RIGHT_562_TM=58.544
PRIMER_LEFT_562_GC_PERCENT=45.455
PRIMER_RIGHT_562_GC_PERCENT=41.667
PRIMER_LEFT_562_SELF_ANY=4.00
PRIMER_RIGHT_562_SELF_ANY=4.00
PRIMER_LEFT_562_SELF_END=0.00
PRIMER_RIGHT_562_SELF_END=2.00
PRIMER_LEFT_562_END_STABILITY=6.4000
PRIMER_RIGHT_562_END_STABILITY=8.4000
PRIMER_PAIR_562_COMPL_ANY=5.00
PRIMER_PAIR_562_COMPL_END=2.00
PRIMER_PAIR_562_PRODUCT_SIZE=97
PRIMER_PAIR_562_PRODUCT_TM=73.2
PRIMER_PAIR_563_PENALTY=9.502348
PRIMER_LEFT_563_PENALTY=4.059923
PRIMER_RIGHT_563_PENALTY=5.442425
PRIMER_LEFT_563_SEQUENCE=ACACCAGGAGAGGATGGATGTT
PRIMER_RIGHT_563_SEQUENCE=ATGCAAGTTGGTACTCTTCTCAC
PRIMER_LEFT_563=607,22
PRIMER_RIGHT_563=697,23
PRIMER_LEFT_563_TM=62.060
PRIMER_RIGHT_563_TM=57.558
PRIMER_LEFT_563_GC_PERCENT=50.000
PRIMER_RIGHT_563_GC_PERCENT=43.478
PRIMER_LEFT_563_SELF_ANY=4.00
PRIMER_RIGHT_563_SELF_ANY=5.00
PRIMER_LEFT_563_SELF_END=0.00
PRIMER_RIGHT_563_SELF_END=0.00
PRIMER_LEFT_563_END_STABILITY=6.6000
PRIMER_RIGHT_563_END_STABILITY=6.4000
PRIMER_PAIR_563_COMPL_ANY=5.00
PRIMER_PAIR_563_COMPL_END=1.00
PRIMER_PAIR_563_PRODUCT_SIZE=91
PRIMER_PAIR_563_PRODUCT_TM=73.6
PRIMER_PAIR_564_PENALTY=9.551609
PRIMER_LEFT_564_PENALTY=2.606855
PRIMER_RIGHT_564_PENALTY=6.944753
PRIMER_LEFT_564_SEQUENCE=CCAGAATGGGAAGTGGGA
PRIMER_RIGHT_564_SEQUENCE=TTCAGGGTAGACTTCTTGGCAATAC
PRIMER_LEFT_564=340,18
PRIMER_RIGHT_564=436,25
PRIMER_LEFT_564_TM=59.393
PRIMER_RIGHT_564_TM=61.945
PRIMER_LEFT_564_GC_PERCENT=55.556
PRIMER_RIGHT_564_GC_PERCENT=44.000
PRIMER_LEFT_564_SELF_ANY=3.00
PRIMER_RIGHT_564_SELF_ANY=5.00
PRIMER_LEFT_564_SELF_END=0.00
PRIMER_RIGHT_564_SELF_END=3.00
PRIMER_LEFT_564_END_STABILITY=9.7000
PRIMER_RIGHT_564_END_STABILITY=5.6000
PRIMER_PAIR_564_COMPL_ANY=5.00
PRIMER_PAIR_564_COMPL_END=1.00
PRIMER_PAIR_564_PRODUCT_SIZE=97
PRIMER_PAIR_564_PRODUCT_TM=73.6
PRIMER_PAIR_565_PENALTY=9.639648
PRIMER_LEFT_565_PENALTY=4.184062
PRIMER_RIGHT_565_PENALTY=5.455586
PRIMER_LEFT_565_SEQUENCE=GTTTGCGAAACTCATCTTCACTG
PRIMER_RIGHT_565_SEQUENCE=AGTCATGCAAGTTGGTACTCTTCT
PRIMER_LEFT_565=626,23
PRIMER_RIGHT_565=701,24
PRIMER_LEFT_565_TM=61.184
PRIMER_RIGHT_565_TM=58.544
PRIMER_LEFT_565_GC_PERCENT=43.478
PRIMER_RIGHT_565_GC_PERCENT=41.667
PRIMER_LEFT_565_SELF_ANY=6.00
PRIMER_RIGHT_565_SELF_ANY=5.00
PRIMER_LEFT_565_SELF_END=3.00
PRIMER_RIGHT_565_SELF_END=2.00
PRIMER_LEFT_565_END_STABILITY=6.7000
PRIMER_RIGHT_565_END_STABILITY=6.7000
PRIMER_PAIR_565_COMPL_ANY=4.00
PRIMER_PAIR_565_COMPL_END=1.00
PRIMER_PAIR_565_PRODUCT_SIZE=76
PRIMER_PAIR_565_PRODUCT_TM=72.0
PRIMER_PAIR_566_PENALTY=9.672380
PRIMER_LEFT_566_PENALTY=4.216794
PRIMER_RIGHT_566_PENALTY=5.455586
PRIMER_LEFT_566_SEQUENCE=TCTTACACCAGGAGAGGATGGAT
PRIMER_RIGHT_566_SEQUENCE=ATGCAAGTTGGTACTCTTCTCACT
PRIMER_LEFT_566=603,23
PRIMER_RIGHT_566=697,24
PRIMER_LEFT_566_TM=61.217
PRIMER_RIGHT_566_TM=58.544
PRIMER_LEFT_566_GC_PERCENT=47.826
PRIMER_RIGHT_566_GC_PERCENT=41.667
PRIMER_LEFT_566_SELF_ANY=3.00
PRIMER_RIGHT_566_SELF_ANY=5.00
PRIMER_LEFT_566_SELF_END=2.00
PRIMER_RIGHT_566_SELF_END=1.00
PRIMER_LEFT_566_END_STABILITY=8.1000
PRIMER_RIGHT_566_END_STABILITY=6.4000
PRIMER_PAIR_566_COMPL_ANY=5.00
PRIMER_PAIR_566_COMPL_END=3.00
PRIMER_PAIR_566_PRODUCT_SIZE=95
PRIMER_PAIR_566_PRODUCT_TM=73.4
PRIMER_PAIR_567_PENALTY=9.731362
PRIMER_LEFT_567_PENALTY=5.016724
PRIMER_RIGHT_567_PENALTY=4.714638
PRIMER_LEFT_567_SEQUENCE=CTACCGCTGCTTAGTTGGTGAGT
PRIMER_RIGHT_567_SEQUENCE=CATCCTCTCCTGGTGTAAGAAT
PRIMER_LEFT_567=541,23
PRIMER_RIGHT_567=622,22
PRIMER_LEFT_567_TM=62.017
PRIMER_RIGHT_567_TM=57.285
PRIMER_LEFT_567_GC_PERCENT=52.174
PRIMER_RIGHT_567_GC_PERCENT=45.455
PRIMER_LEFT_567_SELF_ANY=3.00
PRIMER_RIGHT_567_SELF_ANY=3.00
PRIMER_LEFT_567_SELF_END=1.00
PRIMER_RIGHT_567_SELF_END=2.00
PRIMER_LEFT_567_END_STABILITY=6.4000
PRIMER_RIGHT_567_END_STABILITY=6.6000
PRIMER_PAIR_567_COMPL_ANY=4.00
PRIMER_PAIR_567_COMPL_END=1.00
PRIMER_PAIR_567_PRODUCT_SIZE=82
PRIMER_PAIR_567_PRODUCT_TM=72.1
PRIMER_PAIR_568_PENALTY=9.747180
PRIMER_LEFT_568_PENALTY=5.455235
PRIMER_RIGHT_568_PENALTY=4.291945
PRIMER_LEFT_568_SEQUENCE=ATCCCCACTTTGTGATTCCCTAC
PRIMER_RIGHT_568_SEQUENCE=TAAGAATTTGCACTTGTCAGGAAC
PRIMER_LEFT_568=522,23
PRIMER_RIGHT_568=607,24
PRIMER_LEFT_568_TM=62.455
PRIMER_RIGHT_568_TM=59.708
PRIMER_LEFT_568_GC_PERCENT=47.826
PRIMER_RIGHT_568_GC_PERCENT=37.500
PRIMER_LEFT_568_SELF_ANY=4.00
PRIMER_RIGHT_568_SELF_ANY=4.00
PRIMER_LEFT_568_SELF_END=1.00
PRIMER_RIGHT_568_SELF_END=1.00
PRIMER_LEFT_568_END_STABILITY=6.9000
PRIMER_RIGHT_568_END_STABILITY=7.9000
PRIMER_PAIR_568_COMPL_ANY=4.00
PRIMER_PAIR_568_COMPL_END=3.00
PRIMER_PAIR_568_PRODUCT_SIZE=86
PRIMER_PAIR_568_PRODUCT_TM=71.5
PRIMER_PAIR_569_PENALTY=9.749247
PRIMER_LEFT_569_PENALTY=4.847028
PRIMER_RIGHT_569_PENALTY=4.902219
PRIMER_LEFT_569_SEQUENCE=TTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_569_SEQUENCE=CACCAGTTCTGGATGGTCACTG
PRIMER_LEFT_569=415,23
PRIMER_RIGHT_569=492,22
PRIMER_LEFT_569_TM=61.847
PRIMER_RIGHT_569_TM=62.902
PRIMER_LEFT_569_GC_PERCENT=47.826
PRIMER_RIGHT_569_GC_PERCENT=54.545
PRIMER_LEFT_569_SELF_ANY=5.00
PRIMER_RIGHT_569_SELF_ANY=6.00
PRIMER_LEFT_569_SELF_END=0.00
PRIMER_RIGHT_569_SELF_END=3.00
PRIMER_LEFT_569_END_STABILITY=6.7000
PRIMER_RIGHT_569_END_STABILITY=6.7000
PRIMER_PAIR_569_COMPL_ANY=5.00
PRIMER_PAIR_569_COMPL_END=3.00
PRIMER_PAIR_569_PRODUCT_SIZE=78
PRIMER_PAIR_569_PRODUCT_TM=72.7
PRIMER_PAIR_570_PENALTY=9.880636
PRIMER_LEFT_570_PENALTY=3.678365
PRIMER_RIGHT_570_PENALTY=6.202271
PRIMER_LEFT_570_SEQUENCE=CCTGACAAGTGCAAATTCTTACA
PRIMER_RIGHT_570_SEQUENCE=TACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_570=587,23
PRIMER_RIGHT_570=686,25
PRIMER_LEFT_570_TM=59.322
PRIMER_RIGHT_570_TM=58.798
PRIMER_LEFT_570_GC_PERCENT=39.130
PRIMER_RIGHT_570_GC_PERCENT=40.000
PRIMER_LEFT_570_SELF_ANY=4.00
PRIMER_RIGHT_570_SELF_ANY=4.00
PRIMER_LEFT_570_SELF_END=3.00
PRIMER_RIGHT_570_SELF_END=0.00
PRIMER_LEFT_570_END_STABILITY=6.0000
PRIMER_RIGHT_570_END_STABILITY=7.0000
PRIMER_PAIR_570_COMPL_ANY=5.00
PRIMER_PAIR_570_COMPL_END=1.00
PRIMER_PAIR_570_PRODUCT_SIZE=100
PRIMER_PAIR_570_PRODUCT_TM=73.6
PRIMER_PAIR_571_PENALTY=9.921431
PRIMER_LEFT_571_PENALTY=4.099075
PRIMER_RIGHT_571_PENALTY=5.822356
PRIMER_LEFT_571_SEQUENCE=AAATTCTTACACCAGGAGAGGATG
PRIMER_RIGHT_571_SEQUENCE=AGTTGGTACTCTTCTCACTGCAT
PRIMER_LEFT_571=599,24
PRIMER_RIGHT_571=692,23
PRIMER_LEFT_571_TM=59.901
PRIMER_RIGHT_571_TM=57.178
PRIMER_LEFT_571_GC_PERCENT=41.667
PRIMER_RIGHT_571_GC_PERCENT=43.478
PRIMER_LEFT_571_SELF_ANY=4.00
PRIMER_RIGHT_571_SELF_ANY=4.00
PRIMER_LEFT_571_SELF_END=0.00
PRIMER_RIGHT_571_SELF_END=2.00
PRIMER_LEFT_571_END_STABILITY=8.1000
PRIMER_RIGHT_571_END_STABILITY=8.4000
PRIMER_PAIR_571_COMPL_ANY=6.00
PRIMER_PAIR_571_COMPL_END=3.00
PRIMER_PAIR_571_PRODUCT_SIZE=94
PRIMER_PAIR_571_PRODUCT_TM=72.7
PRIMER_PAIR_572_PENALTY=9.971012
PRIMER_LEFT_572_PENALTY=7.514914
PRIMER_RIGHT_572_PENALTY=2.456098
PRIMER_LEFT_572_SEQUENCE=AAGAGACATGCAGTGAGAAGAGTACC
PRIMER_RIGHT_572_SEQUENCE=GCAACACACAAACTCTACCCCT
PRIMER_LEFT_572=663,26
PRIMER_RIGHT_572=760,22
PRIMER_LEFT_572_TM=61.515
PRIMER_RIGHT_572_TM=60.456
PRIMER_LEFT_572_GC_PERCENT=46.154
PRIMER_RIGHT_572_GC_PERCENT=50.000
PRIMER_LEFT_572_SELF_ANY=4.00
PRIMER_RIGHT_572_SELF_ANY=2.00
PRIMER_LEFT_572_SELF_END=2.00
PRIMER_RIGHT_572_SELF_END=0.00
PRIMER_LEFT_572_END_STABILITY=6.6000
PRIMER_RIGHT_572_END_STABILITY=10.9000
PRIMER_PAIR_572_COMPL_ANY=5.00
PRIMER_PAIR_572_COMPL_END=2.00
PRIMER_PAIR_572_PRODUCT_SIZE=98
PRIMER_PAIR_572_PRODUCT_TM=74.3
PRIMER_PAIR_573_PENALTY=10.145973
PRIMER_LEFT_573_PENALTY=3.809493
PRIMER_RIGHT_573_PENALTY=6.336480
PRIMER_LEFT_573_SEQUENCE=GACAAGTGCAAATTCTTACACCA
PRIMER_RIGHT_573_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_573=590,23
PRIMER_RIGHT_573=685,25
PRIMER_LEFT_573_TM=59.191
PRIMER_RIGHT_573_TM=61.336
PRIMER_LEFT_573_GC_PERCENT=39.130
PRIMER_RIGHT_573_GC_PERCENT=44.000
PRIMER_LEFT_573_SELF_ANY=5.00
PRIMER_RIGHT_573_SELF_ANY=4.00
PRIMER_LEFT_573_SELF_END=1.00
PRIMER_RIGHT_573_SELF_END=0.00
PRIMER_LEFT_573_END_STABILITY=8.2000
PRIMER_RIGHT_573_END_STABILITY=7.3000
PRIMER_PAIR_573_COMPL_ANY=4.00
PRIMER_PAIR_573_COMPL_END=2.00
PRIMER_PAIR_573_PRODUCT_SIZE=96
PRIMER_PAIR_573_PRODUCT_TM=73.3
PRIMER_PAIR_574_PENALTY=10.149444
PRIMER_LEFT_574_PENALTY=5.829268
PRIMER_RIGHT_574_PENALTY=4.320176
PRIMER_LEFT_574_SEQUENCE=CAATGTGGTAGAAGCCAACCAAC
PRIMER_RIGHT_574_SEQUENCE=GTAGGGAATCACAAAGTGGGGA
PRIMER_LEFT_574=448,23
PRIMER_RIGHT_574=544,22
PRIMER_LEFT_574_TM=62.829
PRIMER_RIGHT_574_TM=62.320
PRIMER_LEFT_574_GC_PERCENT=47.826
PRIMER_RIGHT_574_GC_PERCENT=50.000
PRIMER_LEFT_574_SELF_ANY=4.00
PRIMER_RIGHT_574_SELF_ANY=3.00
PRIMER_LEFT_574_SELF_END=2.00
PRIMER_RIGHT_574_SELF_END=2.00
PRIMER_LEFT_574_END_STABILITY=8.2000
PRIMER_RIGHT_574_END_STABILITY=10.9000
PRIMER_PAIR_574_COMPL_ANY=4.00
PRIMER_PAIR_574_COMPL_END=1.00
PRIMER_PAIR_574_PRODUCT_SIZE=97
PRIMER_PAIR_574_PRODUCT_TM=76.5
PRIMER_PAIR_575_PENALTY=10.149702
PRIMER_LEFT_575_PENALTY=7.300506
PRIMER_RIGHT_575_PENALTY=2.849195
PRIMER_LEFT_575_SEQUENCE=CAAAGAGACATGCAGTGAGAAGAGTA
PRIMER_RIGHT_575_SEQUENCE=CACACAAACTCTACCCCTCGG
PRIMER_LEFT_575=661,26
PRIMER_RIGHT_575=756,21
PRIMER_LEFT_575_TM=61.301
PRIMER_RIGHT_575_TM=61.849
PRIMER_LEFT_575_GC_PERCENT=42.308
PRIMER_RIGHT_575_GC_PERCENT=57.143
PRIMER_LEFT_575_SELF_ANY=4.00
PRIMER_RIGHT_575_SELF_ANY=2.00
PRIMER_LEFT_575_SELF_END=2.00
PRIMER_RIGHT_575_SELF_END=2.00
PRIMER_LEFT_575_END_STABILITY=5.4000
PRIMER_RIGHT_575_END_STABILITY=9.9000
PRIMER_PAIR_575_COMPL_ANY=5.00
PRIMER_PAIR_575_COMPL_END=2.00
PRIMER_PAIR_575_PRODUCT_SIZE=96
PRIMER_PAIR_575_PRODUCT_TM=74.2
PRIMER_PAIR_576_PENALTY=10.165261
PRIMER_LEFT_576_PENALTY=6.483283
PRIMER_RIGHT_576_PENALTY=3.681979
PRIMER_LEFT_576_SEQUENCE=CTGGAGGTACCCACTGATGGTAAT
PRIMER_RIGHT_576_SEQUENCE=TGCATGTTCAGTCTGCCA
PRIMER_LEFT_576=251,24
PRIMER_RIGHT_576=330,18
PRIMER_LEFT_576_TM=62.483
PRIMER_RIGHT_576_TM=58.318
PRIMER_LEFT_576_GC_PERCENT=50.000
PRIMER_RIGHT_576_GC_PERCENT=50.000
PRIMER_LEFT_576_SELF_ANY=6.00
PRIMER_RIGHT_576_SELF_ANY=5.00
PRIMER_LEFT_576_SELF_END=2.00
PRIMER_RIGHT_576_SELF_END=3.00
PRIMER_LEFT_576_END_STABILITY=5.6000
PRIMER_RIGHT_576_END_STABILITY=10.0000
PRIMER_PAIR_576_COMPL_ANY=5.00
PRIMER_PAIR_576_COMPL_END=1.00
PRIMER_PAIR_576_PRODUCT_SIZE=80
PRIMER_PAIR_576_PRODUCT_TM=75.0
PRIMER_PAIR_577_PENALTY=10.172787
PRIMER_LEFT_577_PENALTY=4.099075
PRIMER_RIGHT_577_PENALTY=6.073713
PRIMER_LEFT_577_SEQUENCE=CAAATTCTTACACCAGGAGAGGAT
PRIMER_RIGHT_577_SEQUENCE=CAAGTTGGTACTCTTCTCACTGCAT
PRIMER_LEFT_577=598,24
PRIMER_RIGHT_577=694,25
PRIMER_LEFT_577_TM=59.901
PRIMER_RIGHT_577_TM=61.074
PRIMER_LEFT_577_GC_PERCENT=41.667
PRIMER_RIGHT_577_GC_PERCENT=44.000
PRIMER_LEFT_577_SELF_ANY=4.00
PRIMER_RIGHT_577_SELF_ANY=5.00
PRIMER_LEFT_577_SELF_END=2.00
PRIMER_RIGHT_577_SELF_END=2.00
PRIMER_LEFT_577_END_STABILITY=7.8000
PRIMER_RIGHT_577_END_STABILITY=8.4000
PRIMER_PAIR_577_COMPL_ANY=6.00
PRIMER_PAIR_577_COMPL_END=3.00
PRIMER_PAIR_577_PRODUCT_SIZE=97
PRIMER_PAIR_577_PRODUCT_TM=73.2
PRIMER_PAIR_578_PENALTY=10.188964
PRIMER_LEFT_578_PENALTY=5.194065
PRIMER_RIGHT_578_PENALTY=4.994899
PRIMER_LEFT_578_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGTG
PRIMER_RIGHT_578_SEQUENCE=AACATCCATCCTCTCCTGGTGTA
PRIMER_LEFT_578=548,25
PRIMER_RIGHT_578=628,23
PRIMER_LEFT_578_TM=59.806
PRIMER_RIGHT_578_TM=61.995
PRIMER_LEFT_578_GC_PERCENT=40.000
PRIMER_RIGHT_578_GC_PERCENT=47.826
PRIMER_LEFT_578_SELF_ANY=4.00
PRIMER_RIGHT_578_SELF_ANY=4.00
PRIMER_LEFT_578_SELF_END=0.00
PRIMER_RIGHT_578_SELF_END=2.00
PRIMER_LEFT_578_END_STABILITY=6.7000
PRIMER_RIGHT_578_END_STABILITY=5.4000
PRIMER_PAIR_578_COMPL_ANY=5.00
PRIMER_PAIR_578_COMPL_END=0.00
PRIMER_PAIR_578_PRODUCT_SIZE=81
PRIMER_PAIR_578_PRODUCT_TM=70.7
PRIMER_PAIR_579_PENALTY=10.207732
PRIMER_LEFT_579_PENALTY=4.523430
PRIMER_RIGHT_579_PENALTY=5.684302
PRIMER_LEFT_579_SEQUENCE=ACCAGGAGAGGATGGATGTTTG
PRIMER_RIGHT_579_SEQUENCE=GCAAGTTGGTACTCTTCTCACTGC
PRIMER_LEFT_579=609,22
PRIMER_RIGHT_579=695,24
PRIMER_LEFT_579_TM=62.523
PRIMER_RIGHT_579_TM=61.684
PRIMER_LEFT_579_GC_PERCENT=50.000
PRIMER_RIGHT_579_GC_PERCENT=50.000
PRIMER_LEFT_579_SELF_ANY=3.00
PRIMER_RIGHT_579_SELF_ANY=5.00
PRIMER_LEFT_579_SELF_END=0.00
PRIMER_RIGHT_579_SELF_END=2.00
PRIMER_LEFT_579_END_STABILITY=7.0000
PRIMER_RIGHT_579_END_STABILITY=7.9000
PRIMER_PAIR_579_COMPL_ANY=6.00
PRIMER_PAIR_579_COMPL_END=1.00
PRIMER_PAIR_579_PRODUCT_SIZE=87
PRIMER_PAIR_579_PRODUCT_TM=73.7
PRIMER_PAIR_580_PENALTY=10.225384
PRIMER_LEFT_580_PENALTY=5.338743
PRIMER_RIGHT_580_PENALTY=4.886641
PRIMER_LEFT_580_SEQUENCE=TACCGCTGCTTAGTTGGTGAGTT
PRIMER_RIGHT_580_SEQUENCE=CTCCTGGTGTAAGAATTTGCACTT
PRIMER_LEFT_580=542,23
PRIMER_RIGHT_580=616,24
PRIMER_LEFT_580_TM=62.339
PRIMER_RIGHT_580_TM=60.887
PRIMER_LEFT_580_GC_PERCENT=47.826
PRIMER_RIGHT_580_GC_PERCENT=41.667
PRIMER_LEFT_580_SELF_ANY=3.00
PRIMER_RIGHT_580_SELF_ANY=5.00
PRIMER_LEFT_580_SELF_END=0.00
PRIMER_RIGHT_580_SELF_END=2.00
PRIMER_LEFT_580_END_STABILITY=6.4000
PRIMER_RIGHT_580_END_STABILITY=6.7000
PRIMER_PAIR_580_COMPL_ANY=4.00
PRIMER_PAIR_580_COMPL_END=1.00
PRIMER_PAIR_580_PRODUCT_SIZE=75
PRIMER_PAIR_580_PRODUCT_TM=71.0
PRIMER_PAIR_581_PENALTY=10.229938
PRIMER_LEFT_581_PENALTY=5.937993
PRIMER_RIGHT_581_PENALTY=4.291945
PRIMER_LEFT_581_SEQUENCE=GTGATTCCCTACCGCTGCTTAGT
PRIMER_RIGHT_581_SEQUENCE=GTAAGAATTTGCACTTGTCAGGAA
PRIMER_LEFT_581=533,23
PRIMER_RIGHT_581=608,24
PRIMER_LEFT_581_TM=62.938
PRIMER_RIGHT_581_TM=59.708
PRIMER_LEFT_581_GC_PERCENT=52.174
PRIMER_RIGHT_581_GC_PERCENT=37.500
PRIMER_LEFT_581_SELF_ANY=3.00
PRIMER_RIGHT_581_SELF_ANY=4.00
PRIMER_LEFT_581_SELF_END=1.00
PRIMER_RIGHT_581_SELF_END=0.00
PRIMER_LEFT_581_END_STABILITY=5.7000
PRIMER_RIGHT_581_END_STABILITY=8.2000
PRIMER_PAIR_581_COMPL_ANY=4.00
PRIMER_PAIR_581_COMPL_END=3.00
PRIMER_PAIR_581_PRODUCT_SIZE=76
PRIMER_PAIR_581_PRODUCT_TM=70.9
PRIMER_PAIR_582_PENALTY=10.242055
PRIMER_LEFT_582_PENALTY=4.123386
PRIMER_RIGHT_582_PENALTY=6.118669
PRIMER_LEFT_582_SEQUENCE=CGAAACTCATCTTCACTGGCAC
PRIMER_RIGHT_582_SEQUENCE=CCGTAGTCATGCAAGTTGGTACTC
PRIMER_LEFT_582=631,22
PRIMER_RIGHT_582=705,24
PRIMER_LEFT_582_TM=62.123
PRIMER_RIGHT_582_TM=62.119
PRIMER_LEFT_582_GC_PERCENT=50.000
PRIMER_RIGHT_582_GC_PERCENT=50.000
PRIMER_LEFT_582_SELF_ANY=3.00
PRIMER_RIGHT_582_SELF_ANY=5.00
PRIMER_LEFT_582_SELF_END=1.00
PRIMER_RIGHT_582_SELF_END=2.00
PRIMER_LEFT_582_END_STABILITY=9.4000
PRIMER_RIGHT_582_END_STABILITY=5.4000
PRIMER_PAIR_582_COMPL_ANY=5.00
PRIMER_PAIR_582_COMPL_END=3.00
PRIMER_PAIR_582_PRODUCT_SIZE=75
PRIMER_PAIR_582_PRODUCT_TM=72.7
PRIMER_PAIR_583_PENALTY=10.243737
PRIMER_LEFT_583_PENALTY=3.809493
PRIMER_RIGHT_583_PENALTY=6.434244
PRIMER_LEFT_583_SEQUENCE=TGACAAGTGCAAATTCTTACACC
PRIMER_RIGHT_583_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_583=589,23
PRIMER_RIGHT_583=687,26
PRIMER_LEFT_583_TM=59.191
PRIMER_RIGHT_583_TM=59.566
PRIMER_LEFT_583_GC_PERCENT=39.130
PRIMER_RIGHT_583_GC_PERCENT=42.308
PRIMER_LEFT_583_SELF_ANY=5.00
PRIMER_RIGHT_583_SELF_ANY=4.00
PRIMER_LEFT_583_SELF_END=3.00
PRIMER_RIGHT_583_SELF_END=0.00
PRIMER_LEFT_583_END_STABILITY=7.6000
PRIMER_RIGHT_583_END_STABILITY=7.0000
PRIMER_PAIR_583_COMPL_ANY=5.00
PRIMER_PAIR_583_COMPL_END=0.00
PRIMER_PAIR_583_PRODUCT_SIZE=99
PRIMER_PAIR_583_PRODUCT_TM=73.3
PRIMER_PAIR_584_PENALTY=10.285684
PRIMER_LEFT_584_PENALTY=6.612910
PRIMER_RIGHT_584_PENALTY=3.672773
PRIMER_LEFT_584_SEQUENCE=GAGGTACCCACTGATGGTAATGCT
PRIMER_RIGHT_584_SEQUENCE=TTCCCATTCTGGACATTCA
PRIMER_LEFT_584=254,24
PRIMER_RIGHT_584=351,19
PRIMER_LEFT_584_TM=62.613
PRIMER_RIGHT_584_TM=57.327
PRIMER_LEFT_584_GC_PERCENT=50.000
PRIMER_RIGHT_584_GC_PERCENT=42.105
PRIMER_LEFT_584_SELF_ANY=6.00
PRIMER_RIGHT_584_SELF_ANY=3.00
PRIMER_LEFT_584_SELF_END=0.00
PRIMER_RIGHT_584_SELF_END=2.00
PRIMER_LEFT_584_END_STABILITY=8.1000
PRIMER_RIGHT_584_END_STABILITY=6.9000
PRIMER_PAIR_584_COMPL_ANY=5.00
PRIMER_PAIR_584_COMPL_END=2.00
PRIMER_PAIR_584_PRODUCT_SIZE=98
PRIMER_PAIR_584_PRODUCT_TM=75.1
PRIMER_PAIR_585_PENALTY=10.329938
PRIMER_LEFT_585_PENALTY=2.606855
PRIMER_RIGHT_585_PENALTY=7.723082
PRIMER_LEFT_585_SEQUENCE=TCCAGAATGGGAAGTGGG
PRIMER_RIGHT_585_SEQUENCE=AGTTCAGGGTAGACTTCTTGGCAAT
PRIMER_LEFT_585=339,18
PRIMER_RIGHT_585=438,25
PRIMER_LEFT_585_TM=59.393
PRIMER_RIGHT_585_TM=62.723
PRIMER_LEFT_585_GC_PERCENT=55.556
PRIMER_RIGHT_585_GC_PERCENT=44.000
PRIMER_LEFT_585_SELF_ANY=3.00
PRIMER_RIGHT_585_SELF_ANY=5.00
PRIMER_LEFT_585_SELF_END=0.00
PRIMER_RIGHT_585_SELF_END=3.00
PRIMER_LEFT_585_END_STABILITY=9.4000
PRIMER_RIGHT_585_END_STABILITY=8.4000
PRIMER_PAIR_585_COMPL_ANY=5.00
PRIMER_PAIR_585_COMPL_END=1.00
PRIMER_PAIR_585_PRODUCT_SIZE=100
PRIMER_PAIR_585_PRODUCT_TM=73.6
PRIMER_PAIR_586_PENALTY=10.362638
PRIMER_LEFT_586_PENALTY=3.908647
PRIMER_RIGHT_586_PENALTY=6.453992
PRIMER_LEFT_586_SEQUENCE=CAAGTGCAAATTCTTACACCAGG
PRIMER_RIGHT_586_SEQUENCE=GTTGGTACTCTTCTCACTGCATGTC
PRIMER_LEFT_586=592,23
PRIMER_RIGHT_586=691,25
PRIMER_LEFT_586_TM=60.909
PRIMER_RIGHT_586_TM=61.454
PRIMER_LEFT_586_GC_PERCENT=43.478
PRIMER_RIGHT_586_GC_PERCENT=48.000
PRIMER_LEFT_586_SELF_ANY=5.00
PRIMER_RIGHT_586_SELF_ANY=4.00
PRIMER_LEFT_586_SELF_END=3.00
PRIMER_RIGHT_586_SELF_END=2.00
PRIMER_LEFT_586_END_STABILITY=9.7000
PRIMER_RIGHT_586_END_STABILITY=6.3000
PRIMER_PAIR_586_COMPL_ANY=4.00
PRIMER_PAIR_586_COMPL_END=1.00
PRIMER_PAIR_586_PRODUCT_SIZE=100
PRIMER_PAIR_586_PRODUCT_TM=73.6
PRIMER_PAIR_587_PENALTY=10.418744
PRIMER_LEFT_587_PENALTY=4.184062
PRIMER_RIGHT_587_PENALTY=6.234682
PRIMER_LEFT_587_SEQUENCE=TGTTTGCGAAACTCATCTTCACT
PRIMER_RIGHT_587_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTCTC
PRIMER_LEFT_587=625,23
PRIMER_RIGHT_587=702,26
PRIMER_LEFT_587_TM=61.184
PRIMER_RIGHT_587_TM=60.235
PRIMER_LEFT_587_GC_PERCENT=39.130
PRIMER_RIGHT_587_GC_PERCENT=42.308
PRIMER_LEFT_587_SELF_ANY=6.00
PRIMER_RIGHT_587_SELF_ANY=5.00
PRIMER_LEFT_587_SELF_END=1.00
PRIMER_RIGHT_587_SELF_END=0.00
PRIMER_LEFT_587_END_STABILITY=6.4000
PRIMER_RIGHT_587_END_STABILITY=6.7000
PRIMER_PAIR_587_COMPL_ANY=4.00
PRIMER_PAIR_587_COMPL_END=1.00
PRIMER_PAIR_587_PRODUCT_SIZE=78
PRIMER_PAIR_587_PRODUCT_TM=71.7
PRIMER_PAIR_588_PENALTY=10.453756
PRIMER_LEFT_588_PENALTY=5.348422
PRIMER_RIGHT_588_PENALTY=5.105334
PRIMER_LEFT_588_SEQUENCE=TAGTTGGTGAGTTTGTAAGTGATGC
PRIMER_RIGHT_588_SEQUENCE=CATCCATCCTCTCCTGGTGTAAG
PRIMER_LEFT_588=552,25
PRIMER_RIGHT_588=626,23
PRIMER_LEFT_588_TM=59.652
PRIMER_RIGHT_588_TM=62.105
PRIMER_LEFT_588_GC_PERCENT=40.000
PRIMER_RIGHT_588_GC_PERCENT=52.174
PRIMER_LEFT_588_SELF_ANY=2.00
PRIMER_RIGHT_588_SELF_ANY=3.00
PRIMER_LEFT_588_SELF_END=2.00
PRIMER_RIGHT_588_SELF_END=1.00
PRIMER_LEFT_588_END_STABILITY=8.1000
PRIMER_RIGHT_588_END_STABILITY=5.7000
PRIMER_PAIR_588_COMPL_ANY=4.00
PRIMER_PAIR_588_COMPL_END=1.00
PRIMER_PAIR_588_PRODUCT_SIZE=75
PRIMER_PAIR_588_PRODUCT_TM=70.5
PRIMER_PAIR_589_PENALTY=10.578836
PRIMER_LEFT_589_PENALTY=4.505124
PRIMER_RIGHT_589_PENALTY=6.073713
PRIMER_LEFT_589_SEQUENCE=GATGTTTGCGAAACTCATCTTCA
PRIMER_RIGHT_589_SEQUENCE=ATGCAAGTTGGTACTCTTCTCACTG
PRIMER_LEFT_589=623,23
PRIMER_RIGHT_589=697,25
PRIMER_LEFT_589_TM=61.505
PRIMER_RIGHT_589_TM=61.074
PRIMER_LEFT_589_GC_PERCENT=39.130
PRIMER_RIGHT_589_GC_PERCENT=44.000
PRIMER_LEFT_589_SELF_ANY=6.00
PRIMER_RIGHT_589_SELF_ANY=5.00
PRIMER_LEFT_589_SELF_END=1.00
PRIMER_RIGHT_589_SELF_END=3.00
PRIMER_LEFT_589_END_STABILITY=7.0000
PRIMER_RIGHT_589_END_STABILITY=6.7000
PRIMER_PAIR_589_COMPL_ANY=4.00
PRIMER_PAIR_589_COMPL_END=2.00
PRIMER_PAIR_589_PRODUCT_SIZE=75
PRIMER_PAIR_589_PRODUCT_TM=71.6
PRIMER_PAIR_590_PENALTY=10.597143
PRIMER_LEFT_590_PENALTY=4.523430
PRIMER_RIGHT_590_PENALTY=6.073713
PRIMER_LEFT_590_SEQUENCE=CACCAGGAGAGGATGGATGTTT
PRIMER_RIGHT_590_SEQUENCE=CATGCAAGTTGGTACTCTTCTCACT
PRIMER_LEFT_590=608,22
PRIMER_RIGHT_590=698,25
PRIMER_LEFT_590_TM=62.523
PRIMER_RIGHT_590_TM=61.074
PRIMER_LEFT_590_GC_PERCENT=50.000
PRIMER_RIGHT_590_GC_PERCENT=44.000
PRIMER_LEFT_590_SELF_ANY=3.00
PRIMER_RIGHT_590_SELF_ANY=5.00
PRIMER_LEFT_590_SELF_END=0.00
PRIMER_RIGHT_590_SELF_END=1.00
PRIMER_LEFT_590_END_STABILITY=7.0000
PRIMER_RIGHT_590_END_STABILITY=6.4000
PRIMER_PAIR_590_COMPL_ANY=5.00
PRIMER_PAIR_590_COMPL_END=0.00
PRIMER_PAIR_590_PRODUCT_SIZE=91
PRIMER_PAIR_590_PRODUCT_TM=74.0
PRIMER_PAIR_591_PENALTY=10.630119
PRIMER_LEFT_591_PENALTY=5.817794
PRIMER_RIGHT_591_PENALTY=4.812325
PRIMER_LEFT_591_SEQUENCE=ATCACCAATGTGGTAGAAGCCAA
PRIMER_RIGHT_591_SEQUENCE=GATGGGTCTTGCACTGCT
PRIMER_LEFT_591=443,23
PRIMER_RIGHT_591=524,18
PRIMER_LEFT_591_TM=62.818
PRIMER_RIGHT_591_TM=57.188
PRIMER_LEFT_591_GC_PERCENT=43.478
PRIMER_RIGHT_591_GC_PERCENT=55.556
PRIMER_LEFT_591_SELF_ANY=6.00
PRIMER_RIGHT_591_SELF_ANY=5.00
PRIMER_LEFT_591_SELF_END=0.00
PRIMER_RIGHT_591_SELF_END=3.00
PRIMER_LEFT_591_END_STABILITY=10.0000
PRIMER_RIGHT_591_END_STABILITY=8.2000
PRIMER_PAIR_591_COMPL_ANY=4.00
PRIMER_PAIR_591_COMPL_END=3.00
PRIMER_PAIR_591_PRODUCT_SIZE=82
PRIMER_PAIR_591_PRODUCT_TM=75.6
PRIMER_PAIR_592_PENALTY=10.639515
PRIMER_LEFT_592_PENALTY=4.095371
PRIMER_RIGHT_592_PENALTY=6.544143
PRIMER_LEFT_592_SEQUENCE=GACAAGTGCAAATTCTTACACCAG
PRIMER_RIGHT_592_SEQUENCE=TACTCTTCTCACTGCATGTCTCTT
PRIMER_LEFT_592=590,24
PRIMER_RIGHT_592=686,24
PRIMER_LEFT_592_TM=60.095
PRIMER_RIGHT_592_TM=57.456
PRIMER_LEFT_592_GC_PERCENT=41.667
PRIMER_RIGHT_592_GC_PERCENT=41.667
PRIMER_LEFT_592_SELF_ANY=5.00
PRIMER_RIGHT_592_SELF_ANY=4.00
PRIMER_LEFT_592_SELF_END=3.00
PRIMER_RIGHT_592_SELF_END=0.00
PRIMER_LEFT_592_END_STABILITY=7.9000
PRIMER_RIGHT_592_END_STABILITY=6.7000
PRIMER_PAIR_592_COMPL_ANY=4.00
PRIMER_PAIR_592_COMPL_END=1.00
PRIMER_PAIR_592_PRODUCT_SIZE=97
PRIMER_PAIR_592_PRODUCT_TM=73.2
PRIMER_PAIR_593_PENALTY=10.696596
PRIMER_LEFT_593_PENALTY=4.131746
PRIMER_RIGHT_593_PENALTY=6.564850
PRIMER_LEFT_593_SEQUENCE=CAAATTCTTACACCAGGAGAGG
PRIMER_RIGHT_593_SEQUENCE=TTGGTACTCTTCTCACTGCATGTCT
PRIMER_LEFT_593=598,22
PRIMER_RIGHT_593=690,25
PRIMER_LEFT_593_TM=57.868
PRIMER_RIGHT_593_TM=61.565
PRIMER_LEFT_593_GC_PERCENT=45.455
PRIMER_RIGHT_593_GC_PERCENT=44.000
PRIMER_LEFT_593_SELF_ANY=4.00
PRIMER_RIGHT_593_SELF_ANY=4.00
PRIMER_LEFT_593_SELF_END=1.00
PRIMER_RIGHT_593_SELF_END=0.00
PRIMER_LEFT_593_END_STABILITY=7.9000
PRIMER_RIGHT_593_END_STABILITY=6.4000
PRIMER_PAIR_593_COMPL_ANY=5.00
PRIMER_PAIR_593_COMPL_END=3.00
PRIMER_PAIR_593_PRODUCT_SIZE=93
PRIMER_PAIR_593_PRODUCT_TM=72.8
PRIMER_PAIR_594_PENALTY=10.783912
PRIMER_LEFT_594_PENALTY=4.095371
PRIMER_RIGHT_594_PENALTY=6.688541
PRIMER_LEFT_594_SEQUENCE=CTGACAAGTGCAAATTCTTACACC
PRIMER_RIGHT_594_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTT
PRIMER_LEFT_594=588,24
PRIMER_RIGHT_594=687,25
PRIMER_LEFT_594_TM=60.095
PRIMER_RIGHT_594_TM=58.311
PRIMER_LEFT_594_GC_PERCENT=41.667
PRIMER_RIGHT_594_GC_PERCENT=44.000
PRIMER_LEFT_594_SELF_ANY=5.00
PRIMER_RIGHT_594_SELF_ANY=4.00
PRIMER_LEFT_594_SELF_END=3.00
PRIMER_RIGHT_594_SELF_END=0.00
PRIMER_LEFT_594_END_STABILITY=7.6000
PRIMER_RIGHT_594_END_STABILITY=6.7000
PRIMER_PAIR_594_COMPL_ANY=5.00
PRIMER_PAIR_594_COMPL_END=0.00
PRIMER_PAIR_594_PRODUCT_SIZE=100
PRIMER_PAIR_594_PRODUCT_TM=73.6
PRIMER_PAIR_595_PENALTY=10.917192
PRIMER_LEFT_595_PENALTY=5.704162
PRIMER_RIGHT_595_PENALTY=5.213030
PRIMER_LEFT_595_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTAAG
PRIMER_RIGHT_595_SEQUENCE=TCCATCCTCTCCTGGTGTAAGAA
PRIMER_LEFT_595=546,25
PRIMER_RIGHT_595=624,23
PRIMER_LEFT_595_TM=60.704
PRIMER_RIGHT_595_TM=62.213
PRIMER_LEFT_595_GC_PERCENT=44.000
PRIMER_RIGHT_595_GC_PERCENT=47.826
PRIMER_LEFT_595_SELF_ANY=4.00
PRIMER_RIGHT_595_SELF_ANY=3.00
PRIMER_LEFT_595_SELF_END=0.00
PRIMER_RIGHT_595_SELF_END=1.00
PRIMER_LEFT_595_END_STABILITY=5.7000
PRIMER_RIGHT_595_END_STABILITY=7.0000
PRIMER_PAIR_595_COMPL_ANY=4.00
PRIMER_PAIR_595_COMPL_END=1.00
PRIMER_PAIR_595_PRODUCT_SIZE=79
PRIMER_PAIR_595_PRODUCT_TM=71.5
PRIMER_PAIR_596_PENALTY=10.932548
PRIMER_LEFT_596_PENALTY=4.587183
PRIMER_RIGHT_596_PENALTY=6.345365
PRIMER_LEFT_596_SEQUENCE=TTGCGAAACTCATCTTCACTGG
PRIMER_RIGHT_596_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTC
PRIMER_LEFT_596=628,22
PRIMER_RIGHT_596=702,24
PRIMER_LEFT_596_TM=62.587
PRIMER_RIGHT_596_TM=57.655
PRIMER_LEFT_596_GC_PERCENT=45.455
PRIMER_RIGHT_596_GC_PERCENT=41.667
PRIMER_LEFT_596_SELF_ANY=4.00
PRIMER_RIGHT_596_SELF_ANY=5.00
PRIMER_LEFT_596_SELF_END=1.00
PRIMER_RIGHT_596_SELF_END=0.00
PRIMER_LEFT_596_END_STABILITY=7.9000
PRIMER_RIGHT_596_END_STABILITY=6.7000
PRIMER_PAIR_596_COMPL_ANY=4.00
PRIMER_PAIR_596_COMPL_END=1.00
PRIMER_PAIR_596_PRODUCT_SIZE=75
PRIMER_PAIR_596_PRODUCT_TM=71.6
PRIMER_PAIR_597_PENALTY=11.146085
PRIMER_LEFT_597_PENALTY=2.612614
PRIMER_RIGHT_597_PENALTY=8.533470
PRIMER_LEFT_597_SEQUENCE=CCATCAGGGACCAAAACCTG
PRIMER_RIGHT_597_SEQUENCE=GATCTGCAGTTCAGGGTAGACTTCTT
PRIMER_LEFT_597=365,20
PRIMER_RIGHT_597=445,26
PRIMER_LEFT_597_TM=62.613
PRIMER_RIGHT_597_TM=62.533
PRIMER_LEFT_597_GC_PERCENT=55.000
PRIMER_RIGHT_597_GC_PERCENT=46.154
PRIMER_LEFT_597_SELF_ANY=4.00
PRIMER_RIGHT_597_SELF_ANY=8.00
PRIMER_LEFT_597_SELF_END=1.00
PRIMER_RIGHT_597_SELF_END=2.00
PRIMER_LEFT_597_END_STABILITY=7.9000
PRIMER_RIGHT_597_END_STABILITY=7.0000
PRIMER_PAIR_597_COMPL_ANY=4.00
PRIMER_PAIR_597_COMPL_END=1.00
PRIMER_PAIR_597_PRODUCT_SIZE=81
PRIMER_PAIR_597_PRODUCT_TM=72.7
PRIMER_PAIR_598_PENALTY=11.200197
PRIMER_LEFT_598_PENALTY=2.644351
PRIMER_RIGHT_598_PENALTY=8.555845
PRIMER_LEFT_598_SEQUENCE=ATTCAGATCCATCAGGGACCA
PRIMER_RIGHT_598_SEQUENCE=GTTCAGGGTAGACTTCTTGGCAATAC
PRIMER_LEFT_598=357,21
PRIMER_RIGHT_598=437,26
PRIMER_LEFT_598_TM=61.644
PRIMER_RIGHT_598_TM=62.556
PRIMER_LEFT_598_GC_PERCENT=47.619
PRIMER_RIGHT_598_GC_PERCENT=46.154
PRIMER_LEFT_598_SELF_ANY=5.00
PRIMER_RIGHT_598_SELF_ANY=5.00
PRIMER_LEFT_598_SELF_END=3.00
PRIMER_RIGHT_598_SELF_END=3.00
PRIMER_LEFT_598_END_STABILITY=7.9000
PRIMER_RIGHT_598_END_STABILITY=5.6000
PRIMER_PAIR_598_COMPL_ANY=3.00
PRIMER_PAIR_598_COMPL_END=0.00
PRIMER_PAIR_598_PRODUCT_SIZE=81
PRIMER_PAIR_598_PRODUCT_TM=71.7
PRIMER_PAIR_599_PENALTY=11.235681
PRIMER_LEFT_599_PENALTY=4.340634
PRIMER_RIGHT_599_PENALTY=6.895047
PRIMER_LEFT_599_SEQUENCE=AATTCTTACACCAGGAGAGGATG
PRIMER_RIGHT_599_SEQUENCE=TCATGCAAGTTGGTACTCTTCTCAC
PRIMER_LEFT_599=600,23
PRIMER_RIGHT_599=699,25
PRIMER_LEFT_599_TM=58.659
PRIMER_RIGHT_599_TM=61.895
PRIMER_LEFT_599_GC_PERCENT=43.478
PRIMER_RIGHT_599_GC_PERCENT=44.000
PRIMER_LEFT_599_SELF_ANY=4.00
PRIMER_RIGHT_599_SELF_ANY=5.00
PRIMER_LEFT_599_SELF_END=0.00
PRIMER_RIGHT_599_SELF_END=0.00
PRIMER_LEFT_599_END_STABILITY=8.1000
PRIMER_RIGHT_599_END_STABILITY=6.4000
PRIMER_PAIR_599_COMPL_ANY=5.00
PRIMER_PAIR_599_COMPL_END=3.00
PRIMER_PAIR_599_PRODUCT_SIZE=100
PRIMER_PAIR_599_PRODUCT_TM=73.2
PRIMER_PAIR_600_PENALTY=11.291370
PRIMER_LEFT_600_PENALTY=2.644351
PRIMER_RIGHT_600_PENALTY=8.647019
PRIMER_LEFT_600_SEQUENCE=GTGGGATTCAGATCCATCAGG
PRIMER_RIGHT_600_SEQUENCE=TTCAGGGTAGACTTCTTGGCAATACT
PRIMER_LEFT_600=352,21
PRIMER_RIGHT_600=436,26
PRIMER_LEFT_600_TM=61.644
PRIMER_RIGHT_600_TM=62.647
PRIMER_LEFT_600_GC_PERCENT=52.381
PRIMER_RIGHT_600_GC_PERCENT=42.308
PRIMER_LEFT_600_SELF_ANY=5.00
PRIMER_RIGHT_600_SELF_ANY=5.00
PRIMER_LEFT_600_SELF_END=2.00
PRIMER_RIGHT_600_SELF_END=2.00
PRIMER_LEFT_600_END_STABILITY=8.2000
PRIMER_RIGHT_600_END_STABILITY=5.3000
PRIMER_PAIR_600_COMPL_ANY=3.00
PRIMER_PAIR_600_COMPL_END=1.00
PRIMER_PAIR_600_PRODUCT_SIZE=85
PRIMER_PAIR_600_PRODUCT_TM=72.6
PRIMER_PAIR_601_PENALTY=11.330204
PRIMER_LEFT_601_PENALTY=4.933928
PRIMER_RIGHT_601_PENALTY=6.396277
PRIMER_LEFT_601_SEQUENCE=AGAGGATGGATGTTTGCG
PRIMER_RIGHT_601_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTCT
PRIMER_LEFT_601=615,18
PRIMER_RIGHT_601=702,25
PRIMER_LEFT_601_TM=57.066
PRIMER_RIGHT_601_TM=58.604
PRIMER_LEFT_601_GC_PERCENT=50.000
PRIMER_RIGHT_601_GC_PERCENT=40.000
PRIMER_LEFT_601_SELF_ANY=2.00
PRIMER_RIGHT_601_SELF_ANY=5.00
PRIMER_LEFT_601_SELF_END=2.00
PRIMER_RIGHT_601_SELF_END=2.00
PRIMER_LEFT_601_END_STABILITY=10.5000
PRIMER_RIGHT_601_END_STABILITY=6.7000
PRIMER_PAIR_601_COMPL_ANY=4.00
PRIMER_PAIR_601_COMPL_END=0.00
PRIMER_PAIR_601_PRODUCT_SIZE=88
PRIMER_PAIR_601_PRODUCT_TM=72.7
PRIMER_PAIR_602_PENALTY=11.404647
PRIMER_LEFT_602_PENALTY=4.326727
PRIMER_RIGHT_602_PENALTY=7.077920
PRIMER_LEFT_602_SEQUENCE=GCAAATTCTTACACCAGGAGAGG
PRIMER_RIGHT_602_SEQUENCE=TGGTACTCTTCTCACTGCATGTCTC
PRIMER_LEFT_602=597,23
PRIMER_RIGHT_602=689,25
PRIMER_LEFT_602_TM=61.327
PRIMER_RIGHT_602_TM=62.078
PRIMER_LEFT_602_GC_PERCENT=47.826
PRIMER_RIGHT_602_GC_PERCENT=48.000
PRIMER_LEFT_602_SELF_ANY=4.00
PRIMER_RIGHT_602_SELF_ANY=4.00
PRIMER_LEFT_602_SELF_END=1.00
PRIMER_RIGHT_602_SELF_END=0.00
PRIMER_LEFT_602_END_STABILITY=7.9000
PRIMER_RIGHT_602_END_STABILITY=6.1000
PRIMER_PAIR_602_COMPL_ANY=5.00
PRIMER_PAIR_602_COMPL_END=2.00
PRIMER_PAIR_602_PRODUCT_SIZE=93
PRIMER_PAIR_602_PRODUCT_TM=73.3
PRIMER_PAIR_603_PENALTY=11.492613
PRIMER_LEFT_603_PENALTY=5.867986
PRIMER_RIGHT_603_PENALTY=5.624627
PRIMER_LEFT_603_SEQUENCE=CGCTGCTTAGTTGGTGAGTTTGT
PRIMER_RIGHT_603_SEQUENCE=ATCCTCTCCTGGTGTAAGAATTT
PRIMER_LEFT_603=545,23
PRIMER_RIGHT_603=621,23
PRIMER_LEFT_603_TM=62.868
PRIMER_RIGHT_603_TM=57.375
PRIMER_LEFT_603_GC_PERCENT=47.826
PRIMER_RIGHT_603_GC_PERCENT=39.130
PRIMER_LEFT_603_SELF_ANY=3.00
PRIMER_RIGHT_603_SELF_ANY=4.00
PRIMER_LEFT_603_SELF_END=0.00
PRIMER_RIGHT_603_SELF_END=3.00
PRIMER_LEFT_603_END_STABILITY=7.0000
PRIMER_RIGHT_603_END_STABILITY=7.2000
PRIMER_PAIR_603_COMPL_ANY=4.00
PRIMER_PAIR_603_COMPL_END=1.00
PRIMER_PAIR_603_PRODUCT_SIZE=77
PRIMER_PAIR_603_PRODUCT_TM=71.3
PRIMER_PAIR_604_PENALTY=11.519840
PRIMER_LEFT_604_PENALTY=4.994899
PRIMER_RIGHT_604_PENALTY=6.524942
PRIMER_LEFT_604_SEQUENCE=TACACCAGGAGAGGATGGATGTT
PRIMER_RIGHT_604_SEQUENCE=GTAGTCATGCAAGTTGGTACTCTTC
PRIMER_LEFT_604=606,23
PRIMER_RIGHT_604=703,25
PRIMER_LEFT_604_TM=61.995
PRIMER_RIGHT_604_TM=58.475
PRIMER_LEFT_604_GC_PERCENT=47.826
PRIMER_RIGHT_604_GC_PERCENT=44.000
PRIMER_LEFT_604_SELF_ANY=4.00
PRIMER_RIGHT_604_SELF_ANY=5.00
PRIMER_LEFT_604_SELF_END=0.00
PRIMER_RIGHT_604_SELF_END=0.00
PRIMER_LEFT_604_END_STABILITY=6.6000
PRIMER_RIGHT_604_END_STABILITY=6.7000
PRIMER_PAIR_604_COMPL_ANY=4.00
PRIMER_PAIR_604_COMPL_END=1.00
PRIMER_PAIR_604_PRODUCT_SIZE=98
PRIMER_PAIR_604_PRODUCT_TM=73.9
PRIMER_PAIR_605_PENALTY=11.609685
PRIMER_LEFT_605_PENALTY=4.714638
PRIMER_RIGHT_605_PENALTY=6.895047
PRIMER_LEFT_605_SEQUENCE=ATTCTTACACCAGGAGAGGATG
PRIMER_RIGHT_605_SEQUENCE=GTCATGCAAGTTGGTACTCTTCTCA
PRIMER_LEFT_605=601,22
PRIMER_RIGHT_605=700,25
PRIMER_LEFT_605_TM=57.285
PRIMER_RIGHT_605_TM=61.895
PRIMER_LEFT_605_GC_PERCENT=45.455
PRIMER_RIGHT_605_GC_PERCENT=44.000
PRIMER_LEFT_605_SELF_ANY=3.00
PRIMER_RIGHT_605_SELF_ANY=5.00
PRIMER_LEFT_605_SELF_END=0.00
PRIMER_RIGHT_605_SELF_END=1.00
PRIMER_LEFT_605_END_STABILITY=8.1000
PRIMER_RIGHT_605_END_STABILITY=6.7000
PRIMER_PAIR_605_COMPL_ANY=5.00
PRIMER_PAIR_605_COMPL_END=3.00
PRIMER_PAIR_605_PRODUCT_SIZE=100
PRIMER_PAIR_605_PRODUCT_TM=73.6
PRIMER_PAIR_606_PENALTY=11.636080
PRIMER_LEFT_606_PENALTY=4.994899
PRIMER_RIGHT_606_PENALTY=6.641181
PRIMER_LEFT_606_SEQUENCE=TTACACCAGGAGAGGATGGATGT
PRIMER_RIGHT_606_SEQUENCE=GTAGTCATGCAAGTTGGTACTCTTCT
PRIMER_LEFT_606=605,23
PRIMER_RIGHT_606=703,26
PRIMER_LEFT_606_TM=61.995
PRIMER_RIGHT_606_TM=59.359
PRIMER_LEFT_606_GC_PERCENT=47.826
PRIMER_RIGHT_606_GC_PERCENT=42.308
PRIMER_LEFT_606_SELF_ANY=4.00
PRIMER_RIGHT_606_SELF_ANY=5.00
PRIMER_LEFT_606_SELF_END=0.00
PRIMER_RIGHT_606_SELF_END=2.00
PRIMER_LEFT_606_END_STABILITY=6.3000
PRIMER_RIGHT_606_END_STABILITY=6.7000
PRIMER_PAIR_606_COMPL_ANY=5.00
PRIMER_PAIR_606_COMPL_END=2.00
PRIMER_PAIR_606_PRODUCT_SIZE=99
PRIMER_PAIR_606_PRODUCT_TM=73.7
PRIMER_PAIR_607_PENALTY=11.787753
PRIMER_LEFT_607_PENALTY=4.487247
PRIMER_RIGHT_607_PENALTY=7.300506
PRIMER_LEFT_607_SEQUENCE=CAAGTGCAAATTCTTACACCAG
PRIMER_RIGHT_607_SEQUENCE=TACTCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_607=592,22
PRIMER_RIGHT_607=686,26
PRIMER_LEFT_607_TM=57.513
PRIMER_RIGHT_607_TM=61.301
PRIMER_LEFT_607_GC_PERCENT=40.909
PRIMER_RIGHT_607_GC_PERCENT=42.308
PRIMER_LEFT_607_SELF_ANY=5.00
PRIMER_RIGHT_607_SELF_ANY=4.00
PRIMER_LEFT_607_SELF_END=3.00
PRIMER_RIGHT_607_SELF_END=0.00
PRIMER_LEFT_607_END_STABILITY=7.9000
PRIMER_RIGHT_607_END_STABILITY=7.3000
PRIMER_PAIR_607_COMPL_ANY=4.00
PRIMER_PAIR_607_COMPL_END=1.00
PRIMER_PAIR_607_PRODUCT_SIZE=95
PRIMER_PAIR_607_PRODUCT_TM=73.0
PRIMER_PAIR_608_PENALTY=12.139743
PRIMER_LEFT_608_PENALTY=4.624828
PRIMER_RIGHT_608_PENALTY=7.514914
PRIMER_LEFT_608_SEQUENCE=ACAAGTGCAAATTCTTACACCAG
PRIMER_RIGHT_608_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCTT
PRIMER_LEFT_608=591,23
PRIMER_RIGHT_608=688,26
PRIMER_LEFT_608_TM=58.375
PRIMER_RIGHT_608_TM=61.515
PRIMER_LEFT_608_GC_PERCENT=39.130
PRIMER_RIGHT_608_GC_PERCENT=46.154
PRIMER_LEFT_608_SELF_ANY=5.00
PRIMER_RIGHT_608_SELF_ANY=4.00
PRIMER_LEFT_608_SELF_END=3.00
PRIMER_RIGHT_608_SELF_END=0.00
PRIMER_LEFT_608_END_STABILITY=7.9000
PRIMER_RIGHT_608_END_STABILITY=6.7000
PRIMER_PAIR_608_COMPL_ANY=4.00
PRIMER_PAIR_608_COMPL_END=1.00
PRIMER_PAIR_608_PRODUCT_SIZE=98
PRIMER_PAIR_608_PRODUCT_TM=73.4
PRIMER_PAIR_609_PENALTY=12.368775
PRIMER_LEFT_609_PENALTY=6.015654
PRIMER_RIGHT_609_PENALTY=6.353121
PRIMER_LEFT_609_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTGA
PRIMER_RIGHT_609_SEQUENCE=TCCATCCTCTCCTGGTGTAAGAAT
PRIMER_LEFT_609=549,25
PRIMER_RIGHT_609=624,24
PRIMER_LEFT_609_TM=58.984
PRIMER_RIGHT_609_TM=62.353
PRIMER_LEFT_609_GC_PERCENT=40.000
PRIMER_RIGHT_609_GC_PERCENT=45.833
PRIMER_LEFT_609_SELF_ANY=4.00
PRIMER_RIGHT_609_SELF_ANY=3.00
PRIMER_LEFT_609_SELF_END=1.00
PRIMER_RIGHT_609_SELF_END=2.00
PRIMER_LEFT_609_END_STABILITY=6.4000
PRIMER_RIGHT_609_END_STABILITY=6.6000
PRIMER_PAIR_609_COMPL_ANY=4.00
PRIMER_PAIR_609_COMPL_END=1.00
PRIMER_PAIR_609_PRODUCT_SIZE=76
PRIMER_PAIR_609_PRODUCT_TM=70.9
PRIMER_PAIR_610_PENALTY=12.415579
PRIMER_LEFT_610_PENALTY=9.559679
PRIMER_RIGHT_610_PENALTY=2.855901
PRIMER_LEFT_610_SEQUENCE=AAAGAGACATGCAGTGAGAAGAGTACC
PRIMER_RIGHT_610_SEQUENCE=CACAAACTCTACCCCTCGG
PRIMER_LEFT_610=662,27
PRIMER_RIGHT_610=754,19
PRIMER_LEFT_610_TM=62.560
PRIMER_RIGHT_610_TM=58.144
PRIMER_LEFT_610_GC_PERCENT=44.444
PRIMER_RIGHT_610_GC_PERCENT=57.895
PRIMER_LEFT_610_SELF_ANY=4.00
PRIMER_RIGHT_610_SELF_ANY=2.00
PRIMER_LEFT_610_SELF_END=2.00
PRIMER_RIGHT_610_SELF_END=2.00
PRIMER_LEFT_610_END_STABILITY=6.6000
PRIMER_RIGHT_610_END_STABILITY=9.9000
PRIMER_PAIR_610_COMPL_ANY=5.00
PRIMER_PAIR_610_COMPL_END=2.00
PRIMER_PAIR_610_PRODUCT_SIZE=93
PRIMER_PAIR_610_PRODUCT_TM=73.7
PRIMER_PAIR_611_PENALTY=12.917820
PRIMER_LEFT_611_PENALTY=6.564699
PRIMER_RIGHT_611_PENALTY=6.353121
PRIMER_LEFT_611_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAG
PRIMER_RIGHT_611_SEQUENCE=ATCCATCCTCTCCTGGTGTAAGAA
PRIMER_LEFT_611=547,24
PRIMER_RIGHT_611=625,24
PRIMER_LEFT_611_TM=57.435
PRIMER_RIGHT_611_TM=62.353
PRIMER_LEFT_611_GC_PERCENT=41.667
PRIMER_RIGHT_611_GC_PERCENT=45.833
PRIMER_LEFT_611_SELF_ANY=4.00
PRIMER_RIGHT_611_SELF_ANY=3.00
PRIMER_LEFT_611_SELF_END=0.00
PRIMER_RIGHT_611_SELF_END=1.00
PRIMER_LEFT_611_END_STABILITY=5.7000
PRIMER_RIGHT_611_END_STABILITY=7.0000
PRIMER_PAIR_611_COMPL_ANY=4.00
PRIMER_PAIR_611_COMPL_END=1.00
PRIMER_PAIR_611_PRODUCT_SIZE=79
PRIMER_PAIR_611_PRODUCT_TM=71.0
PRIMER_PAIR_612_PENALTY=13.022098
PRIMER_LEFT_612_PENALTY=5.105334
PRIMER_RIGHT_612_PENALTY=7.916764
PRIMER_LEFT_612_SEQUENCE=CTTACACCAGGAGAGGATGGATG
PRIMER_RIGHT_612_SEQUENCE=GTAGTCATGCAAGTTGGTACTCTTCTC
PRIMER_LEFT_612=604,23
PRIMER_RIGHT_612=703,27
PRIMER_LEFT_612_TM=62.105
PRIMER_RIGHT_612_TM=60.917
PRIMER_LEFT_612_GC_PERCENT=52.174
PRIMER_RIGHT_612_GC_PERCENT=44.444
PRIMER_LEFT_612_SELF_ANY=3.00
PRIMER_RIGHT_612_SELF_ANY=5.00
PRIMER_LEFT_612_SELF_END=0.00
PRIMER_RIGHT_612_SELF_END=0.00
PRIMER_LEFT_612_END_STABILITY=8.1000
PRIMER_RIGHT_612_END_STABILITY=6.7000
PRIMER_PAIR_612_COMPL_ANY=5.00
PRIMER_PAIR_612_COMPL_END=2.00
PRIMER_PAIR_612_PRODUCT_SIZE=100
PRIMER_PAIR_612_PRODUCT_TM=74.0
PRIMER_PAIR_613_PENALTY=13.033059
PRIMER_LEFT_613_PENALTY=5.172847
PRIMER_RIGHT_613_PENALTY=7.860212
PRIMER_LEFT_613_SEQUENCE=GGATGTTTGCGAAACTCATCTTC
PRIMER_RIGHT_613_SEQUENCE=CCGTAGTCATGCAAGTTGGTACTCT
PRIMER_LEFT_613=622,23
PRIMER_RIGHT_613=705,25
PRIMER_LEFT_613_TM=62.173
PRIMER_RIGHT_613_TM=62.860
PRIMER_LEFT_613_GC_PERCENT=43.478
PRIMER_RIGHT_613_GC_PERCENT=48.000
PRIMER_LEFT_613_SELF_ANY=6.00
PRIMER_RIGHT_613_SELF_ANY=5.00
PRIMER_LEFT_613_SELF_END=3.00
PRIMER_RIGHT_613_SELF_END=0.00
PRIMER_LEFT_613_END_STABILITY=6.7000
PRIMER_RIGHT_613_END_STABILITY=6.1000
PRIMER_PAIR_613_COMPL_ANY=4.00
PRIMER_PAIR_613_COMPL_END=1.00
PRIMER_PAIR_613_PRODUCT_SIZE=84
PRIMER_PAIR_613_PRODUCT_TM=73.3
PRIMER_PAIR_614_PENALTY=13.125715
PRIMER_LEFT_614_PENALTY=4.886641
PRIMER_RIGHT_614_PENALTY=8.239075
PRIMER_LEFT_614_SEQUENCE=AAGTGCAAATTCTTACACCAGGAG
PRIMER_RIGHT_614_SEQUENCE=GTTGGTACTCTTCTCACTGCATGTCT
PRIMER_LEFT_614=593,24
PRIMER_RIGHT_614=691,26
PRIMER_LEFT_614_TM=60.887
PRIMER_RIGHT_614_TM=62.239
PRIMER_LEFT_614_GC_PERCENT=41.667
PRIMER_RIGHT_614_GC_PERCENT=46.154
PRIMER_LEFT_614_SELF_ANY=5.00
PRIMER_RIGHT_614_SELF_ANY=4.00
PRIMER_LEFT_614_SELF_END=3.00
PRIMER_RIGHT_614_SELF_END=0.00
PRIMER_LEFT_614_END_STABILITY=7.9000
PRIMER_RIGHT_614_END_STABILITY=6.4000
PRIMER_PAIR_614_COMPL_ANY=4.00
PRIMER_PAIR_614_COMPL_END=2.00
PRIMER_PAIR_614_PRODUCT_SIZE=99
PRIMER_PAIR_614_PRODUCT_TM=73.3
PRIMER_PAIR_615_PENALTY=13.401367
PRIMER_LEFT_615_PENALTY=6.619809
PRIMER_RIGHT_615_PENALTY=6.781558
PRIMER_LEFT_615_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAGTG
PRIMER_RIGHT_615_SEQUENCE=ACATCCATCCTCTCCTGGTGTAAG
PRIMER_LEFT_615=547,26
PRIMER_RIGHT_615=627,24
PRIMER_LEFT_615_TM=60.620
PRIMER_RIGHT_615_TM=62.782
PRIMER_LEFT_615_GC_PERCENT=42.308
PRIMER_RIGHT_615_GC_PERCENT=50.000
PRIMER_LEFT_615_SELF_ANY=4.00
PRIMER_RIGHT_615_SELF_ANY=4.00
PRIMER_LEFT_615_SELF_END=0.00
PRIMER_RIGHT_615_SELF_END=1.00
PRIMER_LEFT_615_END_STABILITY=6.7000
PRIMER_RIGHT_615_END_STABILITY=5.7000
PRIMER_PAIR_615_COMPL_ANY=5.00
PRIMER_PAIR_615_COMPL_END=2.00
PRIMER_PAIR_615_PRODUCT_SIZE=81
PRIMER_PAIR_615_PRODUCT_TM=71.2
PRIMER_PAIR_616_PENALTY=13.484150
PRIMER_LEFT_616_PENALTY=4.636384
PRIMER_RIGHT_616_PENALTY=8.847766
PRIMER_LEFT_616_SEQUENCE=ACAAGTGCAAATTCTTACACCA
PRIMER_RIGHT_616_SEQUENCE=TGGTACTCTTCTCACTGCATGTCTCT
PRIMER_LEFT_616=591,22
PRIMER_RIGHT_616=689,26
PRIMER_LEFT_616_TM=57.364
PRIMER_RIGHT_616_TM=62.848
PRIMER_LEFT_616_GC_PERCENT=36.364
PRIMER_RIGHT_616_GC_PERCENT=46.154
PRIMER_LEFT_616_SELF_ANY=5.00
PRIMER_RIGHT_616_SELF_ANY=4.00
PRIMER_LEFT_616_SELF_END=1.00
PRIMER_RIGHT_616_SELF_END=0.00
PRIMER_LEFT_616_END_STABILITY=8.2000
PRIMER_RIGHT_616_END_STABILITY=6.4000
PRIMER_PAIR_616_COMPL_ANY=4.00
PRIMER_PAIR_616_COMPL_END=1.00
PRIMER_PAIR_616_PRODUCT_SIZE=99
PRIMER_PAIR_616_PRODUCT_TM=73.3
PRIMER_PAIR_617_PENALTY=13.602746
PRIMER_LEFT_617_PENALTY=5.363671
PRIMER_RIGHT_617_PENALTY=8.239075
PRIMER_LEFT_617_SEQUENCE=GTGCAAATTCTTACACCAGGAGAG
PRIMER_RIGHT_617_SEQUENCE=AGTTGGTACTCTTCTCACTGCATGTC
PRIMER_LEFT_617=595,24
PRIMER_RIGHT_617=692,26
PRIMER_LEFT_617_TM=61.364
PRIMER_RIGHT_617_TM=62.239
PRIMER_LEFT_617_GC_PERCENT=45.833
PRIMER_RIGHT_617_GC_PERCENT=46.154
PRIMER_LEFT_617_SELF_ANY=5.00
PRIMER_RIGHT_617_SELF_ANY=4.00
PRIMER_LEFT_617_SELF_END=0.00
PRIMER_RIGHT_617_SELF_END=2.00
PRIMER_LEFT_617_END_STABILITY=6.4000
PRIMER_RIGHT_617_END_STABILITY=6.3000
PRIMER_PAIR_617_COMPL_ANY=5.00
PRIMER_PAIR_617_COMPL_END=3.00
PRIMER_PAIR_617_PRODUCT_SIZE=98
PRIMER_PAIR_617_PRODUCT_TM=73.4
PRIMER_PAIR_618_PENALTY=13.691286
PRIMER_LEFT_618_PENALTY=6.780955
PRIMER_RIGHT_618_PENALTY=6.910331
PRIMER_LEFT_618_SEQUENCE=CGCTGCTTAGTTGGTGAGTTTGTA
PRIMER_RIGHT_618_SEQUENCE=TCCTCTCCTGGTGTAAGAATTTGC
PRIMER_LEFT_618=545,24
PRIMER_RIGHT_618=620,24
PRIMER_LEFT_618_TM=62.781
PRIMER_RIGHT_618_TM=62.910
PRIMER_LEFT_618_GC_PERCENT=45.833
PRIMER_RIGHT_618_GC_PERCENT=45.833
PRIMER_LEFT_618_SELF_ANY=3.00
PRIMER_RIGHT_618_SELF_ANY=4.00
PRIMER_LEFT_618_SELF_END=2.00
PRIMER_RIGHT_618_SELF_END=2.00
PRIMER_LEFT_618_END_STABILITY=6.0000
PRIMER_RIGHT_618_END_STABILITY=8.8000
PRIMER_PAIR_618_COMPL_ANY=4.00
PRIMER_PAIR_618_COMPL_END=1.00
PRIMER_PAIR_618_PRODUCT_SIZE=76
PRIMER_PAIR_618_PRODUCT_TM=71.4
PRIMER_PAIR_619_PENALTY=13.766602
PRIMER_LEFT_619_PENALTY=5.213030
PRIMER_RIGHT_619_PENALTY=8.553572
PRIMER_LEFT_619_SEQUENCE=TTCTTACACCAGGAGAGGATGGA
PRIMER_RIGHT_619_SEQUENCE=GTCATGCAAGTTGGTACTCTTCTCAC
PRIMER_LEFT_619=602,23
PRIMER_RIGHT_619=700,26
PRIMER_LEFT_619_TM=62.213
PRIMER_RIGHT_619_TM=62.554
PRIMER_LEFT_619_GC_PERCENT=47.826
PRIMER_RIGHT_619_GC_PERCENT=46.154
PRIMER_LEFT_619_SELF_ANY=3.00
PRIMER_RIGHT_619_SELF_ANY=5.00
PRIMER_LEFT_619_SELF_END=0.00
PRIMER_RIGHT_619_SELF_END=0.00
PRIMER_LEFT_619_END_STABILITY=8.1000
PRIMER_RIGHT_619_END_STABILITY=6.4000
PRIMER_PAIR_619_COMPL_ANY=5.00
PRIMER_PAIR_619_COMPL_END=3.00
PRIMER_PAIR_619_PRODUCT_SIZE=99
PRIMER_PAIR_619_PRODUCT_TM=73.7
PRIMER_PAIR_620_PENALTY=13.853960
PRIMER_LEFT_620_PENALTY=5.374686
PRIMER_RIGHT_620_PENALTY=8.479274
PRIMER_LEFT_620_SEQUENCE=ATGTTTGCGAAACTCATCTTCACT
PRIMER_RIGHT_620_SEQUENCE=CGTAGTCATGCAAGTTGGTACTCTTC
PRIMER_LEFT_620=624,24
PRIMER_RIGHT_620=704,26
PRIMER_LEFT_620_TM=61.375
PRIMER_RIGHT_620_TM=62.479
PRIMER_LEFT_620_GC_PERCENT=37.500
PRIMER_RIGHT_620_GC_PERCENT=46.154
PRIMER_LEFT_620_SELF_ANY=6.00
PRIMER_RIGHT_620_SELF_ANY=5.00
PRIMER_LEFT_620_SELF_END=1.00
PRIMER_RIGHT_620_SELF_END=0.00
PRIMER_LEFT_620_END_STABILITY=6.4000
PRIMER_RIGHT_620_END_STABILITY=6.7000
PRIMER_PAIR_620_COMPL_ANY=4.00
PRIMER_PAIR_620_COMPL_END=1.00
PRIMER_PAIR_620_PRODUCT_SIZE=81
PRIMER_PAIR_620_PRODUCT_TM=72.2
PRIMER_PAIR_621_PENALTY=14.001421
PRIMER_LEFT_621_PENALTY=6.702492
PRIMER_RIGHT_621_PENALTY=7.298929
PRIMER_LEFT_621_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGT
PRIMER_RIGHT_621_SEQUENCE=CATCCTCTCCTGGTGTAAGAATTTG
PRIMER_LEFT_621=548,24
PRIMER_RIGHT_621=622,25
PRIMER_LEFT_621_TM=57.298
PRIMER_RIGHT_621_TM=62.299
PRIMER_LEFT_621_GC_PERCENT=37.500
PRIMER_RIGHT_621_GC_PERCENT=44.000
PRIMER_LEFT_621_SELF_ANY=4.00
PRIMER_RIGHT_621_SELF_ANY=4.00
PRIMER_LEFT_621_SELF_END=1.00
PRIMER_RIGHT_621_SELF_END=1.00
PRIMER_LEFT_621_END_STABILITY=5.7000
PRIMER_RIGHT_621_END_STABILITY=7.2000
PRIMER_PAIR_621_COMPL_ANY=4.00
PRIMER_PAIR_621_COMPL_END=1.00
PRIMER_PAIR_621_PRODUCT_SIZE=75
PRIMER_PAIR_621_PRODUCT_TM=70.5
PRIMER_PAIR_622_PENALTY=14.182676
PRIMER_LEFT_622_PENALTY=6.702492
PRIMER_RIGHT_622_PENALTY=7.480183
PRIMER_LEFT_622_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTG
PRIMER_RIGHT_622_SEQUENCE=ATCCATCCTCTCCTGGTGTAAGAAT
PRIMER_LEFT_622=549,24
PRIMER_RIGHT_622=625,25
PRIMER_LEFT_622_TM=57.298
PRIMER_RIGHT_622_TM=62.480
PRIMER_LEFT_622_GC_PERCENT=41.667
PRIMER_RIGHT_622_GC_PERCENT=44.000
PRIMER_LEFT_622_SELF_ANY=4.00
PRIMER_RIGHT_622_SELF_ANY=3.00
PRIMER_LEFT_622_SELF_END=0.00
PRIMER_RIGHT_622_SELF_END=2.00
PRIMER_LEFT_622_END_STABILITY=6.7000
PRIMER_RIGHT_622_END_STABILITY=6.6000
PRIMER_PAIR_622_COMPL_ANY=4.00
PRIMER_PAIR_622_COMPL_END=0.00
PRIMER_PAIR_622_PRODUCT_SIZE=77
PRIMER_PAIR_622_PRODUCT_TM=70.7
PRIMER_PAIR_623_PENALTY=14.506108
PRIMER_LEFT_623_PENALTY=5.854573
PRIMER_RIGHT_623_PENALTY=8.651535
PRIMER_LEFT_623_SEQUENCE=AATTCTTACACCAGGAGAGGATGG
PRIMER_RIGHT_623_SEQUENCE=TCATGCAAGTTGGTACTCTTCTCACT
PRIMER_LEFT_623=600,24
PRIMER_RIGHT_623=699,26
PRIMER_LEFT_623_TM=61.855
PRIMER_RIGHT_623_TM=62.652
PRIMER_LEFT_623_GC_PERCENT=45.833
PRIMER_RIGHT_623_GC_PERCENT=42.308
PRIMER_LEFT_623_SELF_ANY=4.00
PRIMER_RIGHT_623_SELF_ANY=5.00
PRIMER_LEFT_623_SELF_END=0.00
PRIMER_RIGHT_623_SELF_END=1.00
PRIMER_LEFT_623_END_STABILITY=8.1000
PRIMER_RIGHT_623_END_STABILITY=6.4000
PRIMER_PAIR_623_COMPL_ANY=5.00
PRIMER_PAIR_623_COMPL_END=3.00
PRIMER_PAIR_623_PRODUCT_SIZE=100
PRIMER_PAIR_623_PRODUCT_TM=73.2
PRIMER_PAIR_624_PENALTY=14.571495
PRIMER_LEFT_624_PENALTY=5.606786
PRIMER_RIGHT_624_PENALTY=8.964709
PRIMER_LEFT_624_SEQUENCE=ACAAGTGCAAATTCTTACACCAGG
PRIMER_RIGHT_624_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTTTG
PRIMER_LEFT_624=591,24
PRIMER_RIGHT_624=687,27
PRIMER_LEFT_624_TM=61.607
PRIMER_RIGHT_624_TM=61.965
PRIMER_LEFT_624_GC_PERCENT=41.667
PRIMER_RIGHT_624_GC_PERCENT=44.444
PRIMER_LEFT_624_SELF_ANY=5.00
PRIMER_RIGHT_624_SELF_ANY=4.00
PRIMER_LEFT_624_SELF_END=3.00
PRIMER_RIGHT_624_SELF_END=0.00
PRIMER_LEFT_624_END_STABILITY=9.7000
PRIMER_RIGHT_624_END_STABILITY=7.3000
PRIMER_PAIR_624_COMPL_ANY=4.00
PRIMER_PAIR_624_COMPL_END=1.00
PRIMER_PAIR_624_PRODUCT_SIZE=97
PRIMER_PAIR_624_PRODUCT_TM=73.2
PRIMER_PAIR_625_PENALTY=14.625553
PRIMER_LEFT_625_PENALTY=6.738568
PRIMER_RIGHT_625_PENALTY=7.886984
PRIMER_LEFT_625_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTGAT
PRIMER_RIGHT_625_SEQUENCE=CCATCCTCTCCTGGTGTAAGAATTT
PRIMER_LEFT_625=549,26
PRIMER_RIGHT_625=623,25
PRIMER_LEFT_625_TM=59.261
PRIMER_RIGHT_625_TM=62.887
PRIMER_LEFT_625_GC_PERCENT=38.462
PRIMER_RIGHT_625_GC_PERCENT=44.000
PRIMER_LEFT_625_SELF_ANY=4.00
PRIMER_RIGHT_625_SELF_ANY=4.00
PRIMER_LEFT_625_SELF_END=2.00
PRIMER_RIGHT_625_SELF_END=3.00
PRIMER_LEFT_625_END_STABILITY=6.3000
PRIMER_RIGHT_625_END_STABILITY=7.2000
PRIMER_PAIR_625_COMPL_ANY=4.00
PRIMER_PAIR_625_COMPL_END=1.00
PRIMER_PAIR_625_PRODUCT_SIZE=75
PRIMER_PAIR_625_PRODUCT_TM=71.0
PRIMER_PAIR_626_PENALTY=14.855695
PRIMER_LEFT_626_PENALTY=6.204160
PRIMER_RIGHT_626_PENALTY=8.651535
PRIMER_LEFT_626_SEQUENCE=GATGTTTGCGAAACTCATCTTCAC
PRIMER_RIGHT_626_SEQUENCE=AGTCATGCAAGTTGGTACTCTTCTCA
PRIMER_LEFT_626=623,24
PRIMER_RIGHT_626=701,26
PRIMER_LEFT_626_TM=62.204
PRIMER_RIGHT_626_TM=62.652
PRIMER_LEFT_626_GC_PERCENT=41.667
PRIMER_RIGHT_626_GC_PERCENT=42.308
PRIMER_LEFT_626_SELF_ANY=6.00
PRIMER_RIGHT_626_SELF_ANY=5.00
PRIMER_LEFT_626_SELF_END=3.00
PRIMER_RIGHT_626_SELF_END=1.00
PRIMER_LEFT_626_END_STABILITY=6.7000
PRIMER_RIGHT_626_END_STABILITY=6.7000
PRIMER_PAIR_626_COMPL_ANY=4.00
PRIMER_PAIR_626_COMPL_END=2.00
PRIMER_PAIR_626_PRODUCT_SIZE=79
PRIMER_PAIR_626_PRODUCT_TM=72.0
PRIMER_PAIR_627_PENALTY=14.923350
PRIMER_LEFT_627_PENALTY=5.363671
PRIMER_RIGHT_627_PENALTY=9.559679
PRIMER_LEFT_627_SEQUENCE=AGTGCAAATTCTTACACCAGGAGA
PRIMER_RIGHT_627_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCTTT
PRIMER_LEFT_627=594,24
PRIMER_RIGHT_627=688,27
PRIMER_LEFT_627_TM=61.364
PRIMER_RIGHT_627_TM=62.560
PRIMER_LEFT_627_GC_PERCENT=41.667
PRIMER_RIGHT_627_GC_PERCENT=44.444
PRIMER_LEFT_627_SELF_ANY=5.00
PRIMER_RIGHT_627_SELF_ANY=4.00
PRIMER_LEFT_627_SELF_END=1.00
PRIMER_RIGHT_627_SELF_END=0.00
PRIMER_LEFT_627_END_STABILITY=7.9000
PRIMER_RIGHT_627_END_STABILITY=7.0000
PRIMER_PAIR_627_COMPL_ANY=5.00
PRIMER_PAIR_627_COMPL_END=3.00
PRIMER_PAIR_627_PRODUCT_SIZE=95
PRIMER_PAIR_627_PRODUCT_TM=73.4
PRIMER_PAIR_628_PENALTY=15.585840
PRIMER_LEFT_628_PENALTY=5.624627
PRIMER_RIGHT_628_PENALTY=9.961213
PRIMER_LEFT_628_SEQUENCE=AAATTCTTACACCAGGAGAGGAT
PRIMER_RIGHT_628_SEQUENCE=AGTTGGTACTCTTCTCACTGCATGTCT
PRIMER_LEFT_628=599,23
PRIMER_RIGHT_628=692,27
PRIMER_LEFT_628_TM=57.375
PRIMER_RIGHT_628_TM=62.961
PRIMER_LEFT_628_GC_PERCENT=39.130
PRIMER_RIGHT_628_GC_PERCENT=44.444
PRIMER_LEFT_628_SELF_ANY=4.00
PRIMER_RIGHT_628_SELF_ANY=4.00
PRIMER_LEFT_628_SELF_END=2.00
PRIMER_RIGHT_628_SELF_END=0.00
PRIMER_LEFT_628_END_STABILITY=7.8000
PRIMER_RIGHT_628_END_STABILITY=6.4000
PRIMER_PAIR_628_COMPL_ANY=6.00
PRIMER_PAIR_628_COMPL_END=3.00
PRIMER_PAIR_628_PRODUCT_SIZE=94
PRIMER_PAIR_628_PRODUCT_TM=72.7
PRIMER_PAIR_629_PENALTY=16.356344
PRIMER_LEFT_629_PENALTY=6.781558
PRIMER_RIGHT_629_PENALTY=9.574785
PRIMER_LEFT_629_SEQUENCE=CTTACACCAGGAGAGGATGGATGT
PRIMER_RIGHT_629_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTCTCA
PRIMER_LEFT_629=604,24
PRIMER_RIGHT_629=702,27
PRIMER_LEFT_629_TM=62.782
PRIMER_RIGHT_629_TM=62.575
PRIMER_LEFT_629_GC_PERCENT=50.000
PRIMER_RIGHT_629_GC_PERCENT=40.741
PRIMER_LEFT_629_SELF_ANY=4.00
PRIMER_RIGHT_629_SELF_ANY=5.00
PRIMER_LEFT_629_SELF_END=0.00
PRIMER_RIGHT_629_SELF_END=1.00
PRIMER_LEFT_629_END_STABILITY=6.3000
PRIMER_RIGHT_629_END_STABILITY=6.7000
PRIMER_PAIR_629_COMPL_ANY=5.00
PRIMER_PAIR_629_COMPL_END=1.00
PRIMER_PAIR_629_PRODUCT_SIZE=99
PRIMER_PAIR_629_PRODUCT_TM=73.7
PRIMER_PAIR_630_PENALTY=2.436932
PRIMER_LEFT_630_PENALTY=2.199316
PRIMER_RIGHT_630_PENALTY=0.237616
PRIMER_LEFT_630_SEQUENCE=GGGCTCCGTCAGTTTCCT
PRIMER_RIGHT_630_SEQUENCE=ACCATCAGTGGGTACCTCCA
PRIMER_LEFT_630=43,18
PRIMER_RIGHT_630=271,20
PRIMER_LEFT_630_TM=60.199
PRIMER_RIGHT_630_TM=60.238
PRIMER_LEFT_630_GC_PERCENT=61.111
PRIMER_RIGHT_630_GC_PERCENT=55.000
PRIMER_LEFT_630_SELF_ANY=2.00
PRIMER_RIGHT_630_SELF_ANY=6.00
PRIMER_LEFT_630_SELF_END=0.00
PRIMER_RIGHT_630_SELF_END=2.00
PRIMER_LEFT_630_END_STABILITY=8.2000
PRIMER_RIGHT_630_END_STABILITY=8.2000
PRIMER_PAIR_630_COMPL_ANY=3.00
PRIMER_PAIR_630_COMPL_END=1.00
PRIMER_PAIR_630_PRODUCT_SIZE=229
PRIMER_PAIR_630_PRODUCT_TM=87.7
PRIMER_PAIR_631_PENALTY=2.811275
PRIMER_LEFT_631_PENALTY=2.739036
PRIMER_RIGHT_631_PENALTY=0.072238
PRIMER_LEFT_631_SEQUENCE=AAACTCATCTTCACTGGCACAC
PRIMER_RIGHT_631_SEQUENCE=CCAGACATCCGAGTCATCCT
PRIMER_LEFT_631=633,22
PRIMER_RIGHT_631=826,20
PRIMER_LEFT_631_TM=59.261
PRIMER_RIGHT_631_TM=60.072
PRIMER_LEFT_631_GC_PERCENT=45.455
PRIMER_RIGHT_631_GC_PERCENT=55.000
PRIMER_LEFT_631_SELF_ANY=3.00
PRIMER_RIGHT_631_SELF_ANY=5.00
PRIMER_LEFT_631_SELF_END=0.00
PRIMER_RIGHT_631_SELF_END=0.00
PRIMER_LEFT_631_END_STABILITY=6.4000
PRIMER_RIGHT_631_END_STABILITY=7.8000
PRIMER_PAIR_631_COMPL_ANY=4.00
PRIMER_PAIR_631_COMPL_END=0.00
PRIMER_PAIR_631_PRODUCT_SIZE=194
PRIMER_PAIR_631_PRODUCT_TM=77.9
PRIMER_PAIR_632_PENALTY=3.027861
PRIMER_LEFT_632_PENALTY=2.495254
PRIMER_RIGHT_632_PENALTY=0.532607
PRIMER_LEFT_632_SEQUENCE=GTCAGTTTCCTCGGCAGC
PRIMER_RIGHT_632_SEQUENCE=CATCAGTGGGTACCTCCAGC
PRIMER_LEFT_632=50,18
PRIMER_RIGHT_632=269,20
PRIMER_LEFT_632_TM=59.505
PRIMER_RIGHT_632_TM=60.533
PRIMER_LEFT_632_GC_PERCENT=61.111
PRIMER_RIGHT_632_GC_PERCENT=60.000
PRIMER_LEFT_632_SELF_ANY=3.00
PRIMER_RIGHT_632_SELF_ANY=6.00
PRIMER_LEFT_632_SELF_END=3.00
PRIMER_RIGHT_632_SELF_END=2.00
PRIMER_LEFT_632_END_STABILITY=9.7000
PRIMER_RIGHT_632_END_STABILITY=9.7000
PRIMER_PAIR_632_COMPL_ANY=3.00
PRIMER_PAIR_632_COMPL_END=2.00
PRIMER_PAIR_632_PRODUCT_SIZE=220
PRIMER_PAIR_632_PRODUCT_TM=87.6
PRIMER_PAIR_633_PENALTY=3.140197
PRIMER_LEFT_633_PENALTY=3.028072
PRIMER_RIGHT_633_PENALTY=0.112124
PRIMER_LEFT_633_SEQUENCE=ATCCCACTCGCACAGCAG
PRIMER_RIGHT_633_SEQUENCE=CACAGAACATGGCAATCTGG
PRIMER_LEFT_633=154,18
PRIMER_RIGHT_633=314,20
PRIMER_LEFT_633_TM=61.028
PRIMER_RIGHT_633_TM=60.112
PRIMER_LEFT_633_GC_PERCENT=61.111
PRIMER_RIGHT_633_GC_PERCENT=50.000
PRIMER_LEFT_633_SELF_ANY=2.00
PRIMER_RIGHT_633_SELF_ANY=4.00
PRIMER_LEFT_633_SELF_END=2.00
PRIMER_RIGHT_633_SELF_END=0.00
PRIMER_LEFT_633_END_STABILITY=8.2000
PRIMER_RIGHT_633_END_STABILITY=8.2000
PRIMER_PAIR_633_COMPL_ANY=3.00
PRIMER_PAIR_633_COMPL_END=2.00
PRIMER_PAIR_633_PRODUCT_SIZE=161
PRIMER_PAIR_633_PRODUCT_TM=83.4
PRIMER_PAIR_634_PENALTY=3.288557
PRIMER_LEFT_634_PENALTY=3.216208
PRIMER_RIGHT_634_PENALTY=0.072349
PRIMER_LEFT_634_SEQUENCE=AACTCATCTTCACTGGCACAC
PRIMER_RIGHT_634_SEQUENCE=ACCAGACATCCGAGTCATCC
PRIMER_LEFT_634=634,21
PRIMER_RIGHT_634=827,20
PRIMER_LEFT_634_TM=57.784
PRIMER_RIGHT_634_TM=59.928
PRIMER_LEFT_634_GC_PERCENT=47.619
PRIMER_RIGHT_634_GC_PERCENT=55.000
PRIMER_LEFT_634_SELF_ANY=3.00
PRIMER_RIGHT_634_SELF_ANY=5.00
PRIMER_LEFT_634_SELF_END=0.00
PRIMER_RIGHT_634_SELF_END=0.00
PRIMER_LEFT_634_END_STABILITY=6.4000
PRIMER_RIGHT_634_END_STABILITY=8.1000
PRIMER_PAIR_634_COMPL_ANY=4.00
PRIMER_PAIR_634_COMPL_END=1.00
PRIMER_PAIR_634_PRODUCT_SIZE=194
PRIMER_PAIR_634_PRODUCT_TM=77.9
PRIMER_PAIR_635_PENALTY=3.671011
PRIMER_LEFT_635_PENALTY=3.312402
PRIMER_RIGHT_635_PENALTY=0.358609
PRIMER_LEFT_635_SEQUENCE=GTTCCTGACAAGTGCAAATTCTT
PRIMER_RIGHT_635_SEQUENCE=CAGTGGGCAACACACAAACT
PRIMER_LEFT_635=584,23
PRIMER_RIGHT_635=766,20
PRIMER_LEFT_635_TM=59.688
PRIMER_RIGHT_635_TM=59.641
PRIMER_LEFT_635_GC_PERCENT=39.130
PRIMER_RIGHT_635_GC_PERCENT=50.000
PRIMER_LEFT_635_SELF_ANY=4.00
PRIMER_RIGHT_635_SELF_ANY=3.00
PRIMER_LEFT_635_SELF_END=1.00
PRIMER_RIGHT_635_SELF_END=1.00
PRIMER_LEFT_635_END_STABILITY=7.0000
PRIMER_RIGHT_635_END_STABILITY=6.7000
PRIMER_PAIR_635_COMPL_ANY=3.00
PRIMER_PAIR_635_COMPL_END=1.00
PRIMER_PAIR_635_PRODUCT_SIZE=183
PRIMER_PAIR_635_PRODUCT_TM=77.2
PRIMER_PAIR_636_PENALTY=3.740295
PRIMER_LEFT_636_PENALTY=2.500904
PRIMER_RIGHT_636_PENALTY=1.239392
PRIMER_LEFT_636_SEQUENCE=GCTCCGTCAGTTTCCTCG
PRIMER_RIGHT_636_SEQUENCE=TACCATCAGTGGGTACCTCCA
PRIMER_LEFT_636=45,18
PRIMER_RIGHT_636=272,21
PRIMER_LEFT_636_TM=59.499
PRIMER_RIGHT_636_TM=60.239
PRIMER_LEFT_636_GC_PERCENT=61.111
PRIMER_RIGHT_636_GC_PERCENT=52.381
PRIMER_LEFT_636_SELF_ANY=2.00
PRIMER_RIGHT_636_SELF_ANY=6.00
PRIMER_LEFT_636_SELF_END=2.00
PRIMER_RIGHT_636_SELF_END=2.00
PRIMER_LEFT_636_END_STABILITY=9.9000
PRIMER_RIGHT_636_END_STABILITY=8.2000
PRIMER_PAIR_636_COMPL_ANY=3.00
PRIMER_PAIR_636_COMPL_END=1.00
PRIMER_PAIR_636_PRODUCT_SIZE=228
PRIMER_PAIR_636_PRODUCT_TM=87.5
PRIMER_PAIR_637_PENALTY=3.863012
PRIMER_LEFT_637_PENALTY=2.546814
PRIMER_RIGHT_637_PENALTY=1.316198
PRIMER_LEFT_637_SEQUENCE=CGTCAGTTTCCTCGGCAG
PRIMER_RIGHT_637_SEQUENCE=CCAGCATTACCATCAGTGGG
PRIMER_LEFT_637=49,18
PRIMER_RIGHT_637=279,20
PRIMER_LEFT_637_TM=60.547
PRIMER_RIGHT_637_TM=61.316
PRIMER_LEFT_637_GC_PERCENT=61.111
PRIMER_RIGHT_637_GC_PERCENT=55.000
PRIMER_LEFT_637_SELF_ANY=2.00
PRIMER_RIGHT_637_SELF_ANY=3.00
PRIMER_LEFT_637_SELF_END=2.00
PRIMER_RIGHT_637_SELF_END=1.00
PRIMER_LEFT_637_END_STABILITY=9.7000
PRIMER_RIGHT_637_END_STABILITY=9.4000
PRIMER_PAIR_637_COMPL_ANY=2.00
PRIMER_PAIR_637_COMPL_END=0.00
PRIMER_PAIR_637_PRODUCT_SIZE=231
PRIMER_PAIR_637_PRODUCT_TM=87.3
PRIMER_PAIR_638_PENALTY=4.133102
PRIMER_LEFT_638_PENALTY=2.664693
PRIMER_RIGHT_638_PENALTY=1.468408
PRIMER_LEFT_638_SEQUENCE=CTCCGTCAGTTTCCTCGG
PRIMER_RIGHT_638_SEQUENCE=GCCAGCATTACCATCAGTGG
PRIMER_LEFT_638=46,18
PRIMER_RIGHT_638=280,20
PRIMER_LEFT_638_TM=59.335
PRIMER_RIGHT_638_TM=61.468
PRIMER_LEFT_638_GC_PERCENT=61.111
PRIMER_RIGHT_638_GC_PERCENT=55.000
PRIMER_LEFT_638_SELF_ANY=3.00
PRIMER_RIGHT_638_SELF_ANY=3.00
PRIMER_LEFT_638_SELF_END=2.00
PRIMER_RIGHT_638_SELF_END=3.00
PRIMER_LEFT_638_END_STABILITY=9.9000
PRIMER_RIGHT_638_END_STABILITY=7.9000
PRIMER_PAIR_638_COMPL_ANY=2.00
PRIMER_PAIR_638_COMPL_END=2.00
PRIMER_PAIR_638_PRODUCT_SIZE=235
PRIMER_PAIR_638_PRODUCT_TM=87.4
PRIMER_PAIR_639_PENALTY=4.203374
PRIMER_LEFT_639_PENALTY=4.091249
PRIMER_RIGHT_639_PENALTY=0.112124
PRIMER_LEFT_639_SEQUENCE=GATCCCACTCGCACAGCA
PRIMER_RIGHT_639_SEQUENCE=CCACAGAACATGGCAATCTG
PRIMER_LEFT_639=153,18
PRIMER_RIGHT_639=315,20
PRIMER_LEFT_639_TM=62.091
PRIMER_RIGHT_639_TM=60.112
PRIMER_LEFT_639_GC_PERCENT=61.111
PRIMER_RIGHT_639_GC_PERCENT=50.000
PRIMER_LEFT_639_SELF_ANY=4.00
PRIMER_RIGHT_639_SELF_ANY=4.00
PRIMER_LEFT_639_SELF_END=0.00
PRIMER_RIGHT_639_SELF_END=2.00
PRIMER_LEFT_639_END_STABILITY=8.5000
PRIMER_RIGHT_639_END_STABILITY=6.6000
PRIMER_PAIR_639_COMPL_ANY=3.00
PRIMER_PAIR_639_COMPL_END=2.00
PRIMER_PAIR_639_PRODUCT_SIZE=163
PRIMER_PAIR_639_PRODUCT_TM=83.6
PRIMER_PAIR_640_PENALTY=4.215026
PRIMER_LEFT_640_PENALTY=3.270687
PRIMER_RIGHT_640_PENALTY=0.944339
PRIMER_LEFT_640_SEQUENCE=CTTCACTGGCACACCGTC
PRIMER_RIGHT_640_SEQUENCE=ACATCCGAGTCATCCTCCTC
PRIMER_LEFT_640=641,18
PRIMER_RIGHT_640=822,20
PRIMER_LEFT_640_TM=58.729
PRIMER_RIGHT_640_TM=59.056
PRIMER_LEFT_640_GC_PERCENT=61.111
PRIMER_RIGHT_640_GC_PERCENT=55.000
PRIMER_LEFT_640_SELF_ANY=3.00
PRIMER_RIGHT_640_SELF_ANY=3.00
PRIMER_LEFT_640_SELF_END=1.00
PRIMER_RIGHT_640_SELF_END=0.00
PRIMER_LEFT_640_END_STABILITY=9.6000
PRIMER_RIGHT_640_END_STABILITY=7.9000
PRIMER_PAIR_640_COMPL_ANY=3.00
PRIMER_PAIR_640_COMPL_END=0.00
PRIMER_PAIR_640_PRODUCT_SIZE=182
PRIMER_PAIR_640_PRODUCT_TM=78.0
PRIMER_PAIR_641_PENALTY=4.215026
PRIMER_LEFT_641_PENALTY=3.270687
PRIMER_RIGHT_641_PENALTY=0.944339
PRIMER_LEFT_641_SEQUENCE=TCTTCACTGGCACACCGT
PRIMER_RIGHT_641_SEQUENCE=GACATCCGAGTCATCCTCCT
PRIMER_LEFT_641=640,18
PRIMER_RIGHT_641=823,20
PRIMER_LEFT_641_TM=58.729
PRIMER_RIGHT_641_TM=59.056
PRIMER_LEFT_641_GC_PERCENT=55.556
PRIMER_RIGHT_641_GC_PERCENT=55.000
PRIMER_LEFT_641_SELF_ANY=3.00
PRIMER_RIGHT_641_SELF_ANY=5.00
PRIMER_LEFT_641_SELF_END=3.00
PRIMER_RIGHT_641_SELF_END=0.00
PRIMER_LEFT_641_END_STABILITY=9.3000
PRIMER_RIGHT_641_END_STABILITY=7.9000
PRIMER_PAIR_641_COMPL_ANY=3.00
PRIMER_PAIR_641_COMPL_END=0.00
PRIMER_PAIR_641_PRODUCT_SIZE=184
PRIMER_PAIR_641_PRODUCT_TM=78.0
PRIMER_PAIR_642_PENALTY=4.354791
PRIMER_LEFT_642_PENALTY=3.532432
PRIMER_RIGHT_642_PENALTY=0.822359
PRIMER_LEFT_642_SEQUENCE=TTCCTGACAAGTGCAAATTCT
PRIMER_RIGHT_642_SEQUENCE=CACTTTCTTCAGCCAGTGGG
PRIMER_LEFT_642=585,21
PRIMER_RIGHT_642=779,20
PRIMER_LEFT_642_TM=57.468
PRIMER_RIGHT_642_TM=60.822
PRIMER_LEFT_642_GC_PERCENT=38.095
PRIMER_RIGHT_642_GC_PERCENT=55.000
PRIMER_LEFT_642_SELF_ANY=4.00
PRIMER_RIGHT_642_SELF_ANY=6.00
PRIMER_LEFT_642_SELF_END=2.00
PRIMER_RIGHT_642_SELF_END=3.00
PRIMER_LEFT_642_END_STABILITY=6.6000
PRIMER_RIGHT_642_END_STABILITY=9.4000
PRIMER_PAIR_642_COMPL_ANY=5.00
PRIMER_PAIR_642_COMPL_END=0.00
PRIMER_PAIR_642_PRODUCT_SIZE=195
PRIMER_PAIR_642_PRODUCT_TM=77.2
PRIMER_PAIR_643_PENALTY=4.455354
PRIMER_LEFT_643_PENALTY=3.511015
PRIMER_RIGHT_643_PENALTY=0.944339
PRIMER_LEFT_643_SEQUENCE=AACTCATCTTCACTGGCACACC
PRIMER_RIGHT_643_SEQUENCE=AGACATCCGAGTCATCCTCC
PRIMER_LEFT_643=634,22
PRIMER_RIGHT_643=824,20
PRIMER_LEFT_643_TM=61.511
PRIMER_RIGHT_643_TM=59.056
PRIMER_LEFT_643_GC_PERCENT=50.000
PRIMER_RIGHT_643_GC_PERCENT=55.000
PRIMER_LEFT_643_SELF_ANY=3.00
PRIMER_RIGHT_643_SELF_ANY=5.00
PRIMER_LEFT_643_SELF_END=0.00
PRIMER_RIGHT_643_SELF_END=0.00
PRIMER_LEFT_643_END_STABILITY=7.6000
PRIMER_RIGHT_643_END_STABILITY=9.4000
PRIMER_PAIR_643_COMPL_ANY=4.00
PRIMER_PAIR_643_COMPL_END=0.00
PRIMER_PAIR_643_PRODUCT_SIZE=191
PRIMER_PAIR_643_PRODUCT_TM=77.8
PRIMER_PAIR_644_PENALTY=4.531863
PRIMER_LEFT_644_PENALTY=3.581573
PRIMER_RIGHT_644_PENALTY=0.950290
PRIMER_LEFT_644_SEQUENCE=TCTCGTTCCTGACAAGTGC
PRIMER_RIGHT_644_SEQUENCE=CACCAGACATCCGAGTCATC
PRIMER_LEFT_644=580,19
PRIMER_RIGHT_644=828,20
PRIMER_LEFT_644_TM=57.418
PRIMER_RIGHT_644_TM=59.050
PRIMER_LEFT_644_GC_PERCENT=52.632
PRIMER_RIGHT_644_GC_PERCENT=55.000
PRIMER_LEFT_644_SELF_ANY=3.00
PRIMER_RIGHT_644_SELF_ANY=5.00
PRIMER_LEFT_644_SELF_END=2.00
PRIMER_RIGHT_644_SELF_END=1.00
PRIMER_LEFT_644_END_STABILITY=7.9000
PRIMER_RIGHT_644_END_STABILITY=6.6000
PRIMER_PAIR_644_COMPL_ANY=4.00
PRIMER_PAIR_644_COMPL_END=0.00
PRIMER_PAIR_644_PRODUCT_SIZE=249
PRIMER_PAIR_644_PRODUCT_TM=78.2
PRIMER_PAIR_645_PENALTY=4.585719
PRIMER_LEFT_645_PENALTY=3.629805
PRIMER_RIGHT_645_PENALTY=0.955914
PRIMER_LEFT_645_SEQUENCE=GTTCCTGACAAGTGCAAATTCT
PRIMER_RIGHT_645_SEQUENCE=CCACCAGACATCCGAGTCAT
PRIMER_LEFT_645=584,22
PRIMER_RIGHT_645=829,20
PRIMER_LEFT_645_TM=58.370
PRIMER_RIGHT_645_TM=60.956
PRIMER_LEFT_645_GC_PERCENT=40.909
PRIMER_RIGHT_645_GC_PERCENT=55.000
PRIMER_LEFT_645_SELF_ANY=4.00
PRIMER_RIGHT_645_SELF_ANY=5.00
PRIMER_LEFT_645_SELF_END=2.00
PRIMER_RIGHT_645_SELF_END=3.00
PRIMER_LEFT_645_END_STABILITY=6.6000
PRIMER_RIGHT_645_END_STABILITY=6.3000
PRIMER_PAIR_645_COMPL_ANY=4.00
PRIMER_PAIR_645_COMPL_END=1.00
PRIMER_PAIR_645_PRODUCT_SIZE=246
PRIMER_PAIR_645_PRODUCT_TM=78.3
PRIMER_PAIR_646_PENALTY=4.593173
PRIMER_LEFT_646_PENALTY=3.124765
PRIMER_RIGHT_646_PENALTY=1.468408
PRIMER_LEFT_646_SEQUENCE=GGGCTCCGTCAGTTTCCTC
PRIMER_RIGHT_646_SEQUENCE=GGCCAGCATTACCATCAGTG
PRIMER_LEFT_646=43,19
PRIMER_RIGHT_646=281,20
PRIMER_LEFT_646_TM=62.125
PRIMER_RIGHT_646_TM=61.468
PRIMER_LEFT_646_GC_PERCENT=63.158
PRIMER_RIGHT_646_GC_PERCENT=55.000
PRIMER_LEFT_646_SELF_ANY=2.00
PRIMER_RIGHT_646_SELF_ANY=4.00
PRIMER_LEFT_646_SELF_END=0.00
PRIMER_RIGHT_646_SELF_END=3.00
PRIMER_LEFT_646_END_STABILITY=7.9000
PRIMER_RIGHT_646_END_STABILITY=6.7000
PRIMER_PAIR_646_COMPL_ANY=3.00
PRIMER_PAIR_646_COMPL_END=1.00
PRIMER_PAIR_646_PRODUCT_SIZE=239
PRIMER_PAIR_646_PRODUCT_TM=87.6
PRIMER_PAIR_647_PENALTY=4.607356
PRIMER_LEFT_647_PENALTY=3.683571
PRIMER_RIGHT_647_PENALTY=0.923785
PRIMER_LEFT_647_SEQUENCE=AGCGGTAGGCGAGAGCAC
PRIMER_RIGHT_647_SEQUENCE=ACAGAACATGGCAATCTGGG
PRIMER_LEFT_647=65,18
PRIMER_RIGHT_647=313,20
PRIMER_LEFT_647_TM=61.684
PRIMER_RIGHT_647_TM=60.924
PRIMER_LEFT_647_GC_PERCENT=66.667
PRIMER_RIGHT_647_GC_PERCENT=50.000
PRIMER_LEFT_647_SELF_ANY=2.00
PRIMER_RIGHT_647_SELF_ANY=4.00
PRIMER_LEFT_647_SELF_END=2.00
PRIMER_RIGHT_647_SELF_END=0.00
PRIMER_LEFT_647_END_STABILITY=7.9000
PRIMER_RIGHT_647_END_STABILITY=9.7000
PRIMER_PAIR_647_COMPL_ANY=3.00
PRIMER_PAIR_647_COMPL_END=1.00
PRIMER_PAIR_647_PRODUCT_SIZE=249
PRIMER_PAIR_647_PRODUCT_TM=86.8
PRIMER_PAIR_648_PENALTY=4.721478
PRIMER_LEFT_648_PENALTY=3.633158
PRIMER_RIGHT_648_PENALTY=1.088320
PRIMER_LEFT_648_SEQUENCE=GAAACTCATCTTCACTGGCAC
PRIMER_RIGHT_648_SEQUENCE=AGACATCCGAGTCATCCTCCT
PRIMER_LEFT_648=632,21
PRIMER_RIGHT_648=824,21
PRIMER_LEFT_648_TM=57.367
PRIMER_RIGHT_648_TM=60.088
PRIMER_LEFT_648_GC_PERCENT=47.619
PRIMER_RIGHT_648_GC_PERCENT=52.381
PRIMER_LEFT_648_SELF_ANY=3.00
PRIMER_RIGHT_648_SELF_ANY=5.00
PRIMER_LEFT_648_SELF_END=1.00
PRIMER_RIGHT_648_SELF_END=0.00
PRIMER_LEFT_648_END_STABILITY=9.4000
PRIMER_RIGHT_648_END_STABILITY=7.9000
PRIMER_PAIR_648_COMPL_ANY=4.00
PRIMER_PAIR_648_COMPL_END=0.00
PRIMER_PAIR_648_PRODUCT_SIZE=193
PRIMER_PAIR_648_PRODUCT_TM=77.8
PRIMER_PAIR_649_PENALTY=4.772239
PRIMER_LEFT_649_PENALTY=2.325240
PRIMER_RIGHT_649_PENALTY=2.446999
PRIMER_LEFT_649_SEQUENCE=GCACATGAATGTCCAGAATGG
PRIMER_RIGHT_649_SEQUENCE=GTCACTGGTTGGTTGGCTT
PRIMER_LEFT_649=328,21
PRIMER_RIGHT_649=477,19
PRIMER_LEFT_649_TM=61.325
PRIMER_RIGHT_649_TM=58.553
PRIMER_LEFT_649_GC_PERCENT=47.619
PRIMER_RIGHT_649_GC_PERCENT=52.632
PRIMER_LEFT_649_SELF_ANY=6.00
PRIMER_RIGHT_649_SELF_ANY=3.00
PRIMER_LEFT_649_SELF_END=3.00
PRIMER_RIGHT_649_SELF_END=0.00
PRIMER_LEFT_649_END_STABILITY=8.4000
PRIMER_RIGHT_649_END_STABILITY=9.7000
PRIMER_PAIR_649_COMPL_ANY=4.00
PRIMER_PAIR_649_COMPL_END=0.00
PRIMER_PAIR_649_PRODUCT_SIZE=150
PRIMER_PAIR_649_PRODUCT_TM=75.9
PRIMER_PAIR_650_PENALTY=4.865284
PRIMER_LEFT_650_PENALTY=3.688912
PRIMER_RIGHT_650_PENALTY=1.176371
PRIMER_LEFT_650_SEQUENCE=GTTCCTGACAAGTGCAAATTC
PRIMER_RIGHT_650_SEQUENCE=AGTCATCCTCCTCCGCATC
PRIMER_LEFT_650=584,21
PRIMER_RIGHT_650=815,19
PRIMER_LEFT_650_TM=57.311
PRIMER_RIGHT_650_TM=60.176
PRIMER_LEFT_650_GC_PERCENT=42.857
PRIMER_RIGHT_650_GC_PERCENT=57.895
PRIMER_LEFT_650_SELF_ANY=4.00
PRIMER_RIGHT_650_SELF_ANY=2.00
PRIMER_LEFT_650_SELF_END=2.00
PRIMER_RIGHT_650_SELF_END=0.00
PRIMER_LEFT_650_END_STABILITY=6.9000
PRIMER_RIGHT_650_END_STABILITY=8.1000
PRIMER_PAIR_650_COMPL_ANY=4.00
PRIMER_PAIR_650_COMPL_END=1.00
PRIMER_PAIR_650_PRODUCT_SIZE=232
PRIMER_PAIR_650_PRODUCT_TM=77.8
PRIMER_PAIR_651_PENALTY=4.870781
PRIMER_LEFT_651_PENALTY=3.694409
PRIMER_RIGHT_651_PENALTY=1.176371
PRIMER_LEFT_651_SEQUENCE=AAGTGATGCCCTTCTCGTT
PRIMER_RIGHT_651_SEQUENCE=GAGTCATCCTCCTCCGCAT
PRIMER_LEFT_651=568,19
PRIMER_RIGHT_651=816,19
PRIMER_LEFT_651_TM=57.306
PRIMER_RIGHT_651_TM=60.176
PRIMER_LEFT_651_GC_PERCENT=47.368
PRIMER_RIGHT_651_GC_PERCENT=57.895
PRIMER_LEFT_651_SELF_ANY=4.00
PRIMER_RIGHT_651_SELF_ANY=3.00
PRIMER_LEFT_651_SELF_END=2.00
PRIMER_RIGHT_651_SELF_END=2.00
PRIMER_LEFT_651_END_STABILITY=8.4000
PRIMER_RIGHT_651_END_STABILITY=10.1000
PRIMER_PAIR_651_COMPL_ANY=4.00
PRIMER_PAIR_651_COMPL_END=0.00
PRIMER_PAIR_651_PRODUCT_SIZE=249
PRIMER_PAIR_651_PRODUCT_TM=78.1
PRIMER_PAIR_652_PENALTY=4.919996
PRIMER_LEFT_652_PENALTY=3.362783
PRIMER_RIGHT_652_PENALTY=1.557214
PRIMER_LEFT_652_SEQUENCE=CTCGGGCTCCGTCAGTTT
PRIMER_RIGHT_652_SEQUENCE=CAGCATTACCATCAGTGGGT
PRIMER_LEFT_652=40,18
PRIMER_RIGHT_652=278,20
PRIMER_LEFT_652_TM=61.363
PRIMER_RIGHT_652_TM=58.443
PRIMER_LEFT_652_GC_PERCENT=61.111
PRIMER_RIGHT_652_GC_PERCENT=50.000
PRIMER_LEFT_652_SELF_ANY=4.00
PRIMER_RIGHT_652_SELF_ANY=3.00
PRIMER_LEFT_652_SELF_END=0.00
PRIMER_RIGHT_652_SELF_END=3.00
PRIMER_LEFT_652_END_STABILITY=6.7000
PRIMER_RIGHT_652_END_STABILITY=9.4000
PRIMER_PAIR_652_COMPL_ANY=3.00
PRIMER_PAIR_652_COMPL_END=0.00
PRIMER_PAIR_652_PRODUCT_SIZE=239
PRIMER_PAIR_652_PRODUCT_TM=87.4
PRIMER_PAIR_653_PENALTY=5.030515
PRIMER_LEFT_653_PENALTY=3.774565
PRIMER_RIGHT_653_PENALTY=1.255950
PRIMER_LEFT_653_SEQUENCE=AAGTGATGCCCTTCTCGTTCC
PRIMER_RIGHT_653_SEQUENCE=TCAGCAGAATCCACATTGTCA
PRIMER_LEFT_653=568,21
PRIMER_RIGHT_653=798,21
PRIMER_LEFT_653_TM=62.775
PRIMER_RIGHT_653_TM=60.256
PRIMER_LEFT_653_GC_PERCENT=52.381
PRIMER_RIGHT_653_GC_PERCENT=42.857
PRIMER_LEFT_653_SELF_ANY=4.00
PRIMER_RIGHT_653_SELF_ANY=5.00
PRIMER_LEFT_653_SELF_END=0.00
PRIMER_RIGHT_653_SELF_END=3.00
PRIMER_LEFT_653_END_STABILITY=7.9000
PRIMER_RIGHT_653_END_STABILITY=6.7000
PRIMER_PAIR_653_COMPL_ANY=4.00
PRIMER_PAIR_653_COMPL_END=1.00
PRIMER_PAIR_653_PRODUCT_SIZE=231
PRIMER_PAIR_653_PRODUCT_TM=77.5
PRIMER_PAIR_654_PENALTY=5.034918
PRIMER_LEFT_654_PENALTY=3.708825
PRIMER_RIGHT_654_PENALTY=1.326093
PRIMER_LEFT_654_SEQUENCE=GCCCTTCTCGTTCCTGAC
PRIMER_RIGHT_654_SEQUENCE=GCATCAGCAGAATCCACATT
PRIMER_LEFT_654=575,18
PRIMER_RIGHT_654=801,20
PRIMER_LEFT_654_TM=58.291
PRIMER_RIGHT_654_TM=58.674
PRIMER_LEFT_654_GC_PERCENT=61.111
PRIMER_RIGHT_654_GC_PERCENT=45.000
PRIMER_LEFT_654_SELF_ANY=2.00
PRIMER_RIGHT_654_SELF_ANY=4.00
PRIMER_LEFT_654_SELF_END=1.00
PRIMER_RIGHT_654_SELF_END=2.00
PRIMER_LEFT_654_END_STABILITY=6.4000
PRIMER_RIGHT_654_END_STABILITY=6.6000
PRIMER_PAIR_654_COMPL_ANY=4.00
PRIMER_PAIR_654_COMPL_END=0.00
PRIMER_PAIR_654_PRODUCT_SIZE=227
PRIMER_PAIR_654_PRODUCT_TM=77.9
PRIMER_PAIR_655_PENALTY=5.056684
PRIMER_LEFT_655_PENALTY=2.561455
PRIMER_RIGHT_655_PENALTY=2.495229
PRIMER_LEFT_655_SEQUENCE=GCACATGAATGTCCAGAATG
PRIMER_RIGHT_655_SEQUENCE=GTCACTGGTTGGTTGGCTTCT
PRIMER_LEFT_655=328,20
PRIMER_RIGHT_655=477,21
PRIMER_LEFT_655_TM=57.439
PRIMER_RIGHT_655_TM=61.495
PRIMER_LEFT_655_GC_PERCENT=45.000
PRIMER_RIGHT_655_GC_PERCENT=52.381
PRIMER_LEFT_655_SELF_ANY=6.00
PRIMER_RIGHT_655_SELF_ANY=3.00
PRIMER_LEFT_655_SELF_END=1.00
PRIMER_RIGHT_655_SELF_END=0.00
PRIMER_LEFT_655_END_STABILITY=6.9000
PRIMER_RIGHT_655_END_STABILITY=6.7000
PRIMER_PAIR_655_COMPL_ANY=4.00
PRIMER_PAIR_655_COMPL_END=3.00
PRIMER_PAIR_655_PRODUCT_SIZE=150
PRIMER_PAIR_655_PRODUCT_TM=75.9
PRIMER_PAIR_656_PENALTY=5.090283
PRIMER_LEFT_656_PENALTY=3.892438
PRIMER_RIGHT_656_PENALTY=1.197845
PRIMER_LEFT_656_SEQUENCE=ATCTTCACTGGCACACCG
PRIMER_RIGHT_656_SEQUENCE=TTGTCTTCACTCCCATCTGC
PRIMER_LEFT_656=639,18
PRIMER_RIGHT_656=870,20
PRIMER_LEFT_656_TM=58.108
PRIMER_RIGHT_656_TM=58.802
PRIMER_LEFT_656_GC_PERCENT=55.556
PRIMER_RIGHT_656_GC_PERCENT=50.000
PRIMER_LEFT_656_SELF_ANY=3.00
PRIMER_RIGHT_656_SELF_ANY=2.00
PRIMER_LEFT_656_SELF_END=2.00
PRIMER_RIGHT_656_SELF_END=2.00
PRIMER_LEFT_656_END_STABILITY=9.9000
PRIMER_RIGHT_656_END_STABILITY=8.2000
PRIMER_PAIR_656_COMPL_ANY=3.00
PRIMER_PAIR_656_COMPL_END=2.00
PRIMER_PAIR_656_PRODUCT_SIZE=232
PRIMER_PAIR_656_PRODUCT_TM=78.9
PRIMER_PAIR_657_PENALTY=5.306162
PRIMER_LEFT_657_PENALTY=3.928601
PRIMER_RIGHT_657_PENALTY=1.377561
PRIMER_LEFT_657_SEQUENCE=CGTTCCTGACAAGTGCAAATTC
PRIMER_RIGHT_657_SEQUENCE=AGTGGGCAACACACAAACTC
PRIMER_LEFT_657=583,22
PRIMER_RIGHT_657=765,20
PRIMER_LEFT_657_TM=61.929
PRIMER_RIGHT_657_TM=58.622
PRIMER_LEFT_657_GC_PERCENT=45.455
PRIMER_RIGHT_657_GC_PERCENT=50.000
PRIMER_LEFT_657_SELF_ANY=4.00
PRIMER_RIGHT_657_SELF_ANY=3.00
PRIMER_LEFT_657_SELF_END=2.00
PRIMER_RIGHT_657_SELF_END=0.00
PRIMER_LEFT_657_END_STABILITY=6.9000
PRIMER_RIGHT_657_END_STABILITY=6.4000
PRIMER_PAIR_657_COMPL_ANY=3.00
PRIMER_PAIR_657_COMPL_END=0.00
PRIMER_PAIR_657_PRODUCT_SIZE=183
PRIMER_PAIR_657_PRODUCT_TM=77.2
PRIMER_PAIR_658_PENALTY=5.345528
PRIMER_LEFT_658_PENALTY=3.708825
PRIMER_RIGHT_658_PENALTY=1.636702
PRIMER_LEFT_658_SEQUENCE=GGCTCCGTCAGTTTCCTC
PRIMER_RIGHT_658_SEQUENCE=CAGGCCAGCATTACCATCA
PRIMER_LEFT_658=44,18
PRIMER_RIGHT_658=283,19
PRIMER_LEFT_658_TM=58.291
PRIMER_RIGHT_658_TM=60.637
PRIMER_LEFT_658_GC_PERCENT=61.111
PRIMER_RIGHT_658_GC_PERCENT=52.632
PRIMER_LEFT_658_SELF_ANY=2.00
PRIMER_RIGHT_658_SELF_ANY=4.00
PRIMER_LEFT_658_SELF_END=0.00
PRIMER_RIGHT_658_SELF_END=1.00
PRIMER_LEFT_658_END_STABILITY=7.9000
PRIMER_RIGHT_658_END_STABILITY=6.9000
PRIMER_PAIR_658_COMPL_ANY=3.00
PRIMER_PAIR_658_COMPL_END=0.00
PRIMER_PAIR_658_PRODUCT_SIZE=240
PRIMER_PAIR_658_PRODUCT_TM=87.5
PRIMER_PAIR_659_PENALTY=5.348027
PRIMER_LEFT_659_PENALTY=4.100461
PRIMER_RIGHT_659_PENALTY=1.247566
PRIMER_LEFT_659_SEQUENCE=GAAACTCATCTTCACTGGCACAC
PRIMER_RIGHT_659_SEQUENCE=TTTGTCTTCACTCCCATCTGC
PRIMER_LEFT_659=632,23
PRIMER_RIGHT_659=871,21
PRIMER_LEFT_659_TM=61.100
PRIMER_RIGHT_659_TM=60.248
PRIMER_LEFT_659_GC_PERCENT=47.826
PRIMER_RIGHT_659_GC_PERCENT=47.619
PRIMER_LEFT_659_SELF_ANY=3.00
PRIMER_RIGHT_659_SELF_ANY=2.00
PRIMER_LEFT_659_SELF_END=0.00
PRIMER_RIGHT_659_SELF_END=2.00
PRIMER_LEFT_659_END_STABILITY=6.4000
PRIMER_RIGHT_659_END_STABILITY=8.2000
PRIMER_PAIR_659_COMPL_ANY=3.00
PRIMER_PAIR_659_COMPL_END=2.00
PRIMER_PAIR_659_PRODUCT_SIZE=240
PRIMER_PAIR_659_PRODUCT_TM=78.8
PRIMER_PAIR_660_PENALTY=5.355960
PRIMER_LEFT_660_PENALTY=2.150151
PRIMER_RIGHT_660_PENALTY=3.205809
PRIMER_LEFT_660_SEQUENCE=ATGAATGTCCAGAATGGGAAG
PRIMER_RIGHT_660_SEQUENCE=ACTCACCAACTAAGCAGCGGTA
PRIMER_LEFT_660=332,21
PRIMER_RIGHT_660=563,22
PRIMER_LEFT_660_TM=58.850
PRIMER_RIGHT_660_TM=61.206
PRIMER_LEFT_660_GC_PERCENT=42.857
PRIMER_RIGHT_660_GC_PERCENT=50.000
PRIMER_LEFT_660_SELF_ANY=3.00
PRIMER_RIGHT_660_SELF_ANY=3.00
PRIMER_LEFT_660_SELF_END=0.00
PRIMER_RIGHT_660_SELF_END=2.00
PRIMER_LEFT_660_END_STABILITY=8.2000
PRIMER_RIGHT_660_END_STABILITY=8.9000
PRIMER_PAIR_660_COMPL_ANY=3.00
PRIMER_PAIR_660_COMPL_END=0.00
PRIMER_PAIR_660_PRODUCT_SIZE=232
PRIMER_PAIR_660_PRODUCT_TM=78.3
PRIMER_PAIR_661_PENALTY=5.457774
PRIMER_LEFT_661_PENALTY=2.225062
PRIMER_RIGHT_661_PENALTY=3.232712
PRIMER_LEFT_661_SEQUENCE=ATGAATGTCCAGAATGGGAA
PRIMER_RIGHT_661_SEQUENCE=AAGGGCATCACTTACAAACTCA
PRIMER_LEFT_661=332,20
PRIMER_RIGHT_661=580,22
PRIMER_LEFT_661_TM=57.775
PRIMER_RIGHT_661_TM=58.767
PRIMER_LEFT_661_GC_PERCENT=40.000
PRIMER_RIGHT_661_GC_PERCENT=40.909
PRIMER_LEFT_661_SELF_ANY=3.00
PRIMER_RIGHT_661_SELF_ANY=4.00
PRIMER_LEFT_661_SELF_END=1.00
PRIMER_RIGHT_661_SELF_END=1.00
PRIMER_LEFT_661_END_STABILITY=9.7000
PRIMER_RIGHT_661_END_STABILITY=6.4000
PRIMER_PAIR_661_COMPL_ANY=4.00
PRIMER_PAIR_661_COMPL_END=0.00
PRIMER_PAIR_661_PRODUCT_SIZE=249
PRIMER_PAIR_661_PRODUCT_TM=78.2
PRIMER_PAIR_662_PENALTY=5.465799
PRIMER_LEFT_662_PENALTY=2.171836
PRIMER_RIGHT_662_PENALTY=3.293963
PRIMER_LEFT_662_SEQUENCE=CACATGAATGTCCAGAATGGG
PRIMER_RIGHT_662_SEQUENCE=CAAACTCACCAACTAAGCAGC
PRIMER_LEFT_662=329,21
PRIMER_RIGHT_662=566,21
PRIMER_LEFT_662_TM=61.172
PRIMER_RIGHT_662_TM=57.706
PRIMER_LEFT_662_GC_PERCENT=47.619
PRIMER_RIGHT_662_GC_PERCENT=47.619
PRIMER_LEFT_662_SELF_ANY=6.00
PRIMER_RIGHT_662_SELF_ANY=3.00
PRIMER_LEFT_662_SELF_END=1.00
PRIMER_RIGHT_662_SELF_END=3.00
PRIMER_LEFT_662_END_STABILITY=9.6000
PRIMER_RIGHT_662_END_STABILITY=9.7000
PRIMER_PAIR_662_COMPL_ANY=3.00
PRIMER_PAIR_662_COMPL_END=1.00
PRIMER_PAIR_662_PRODUCT_SIZE=238
PRIMER_PAIR_662_PRODUCT_TM=78.4
PRIMER_PAIR_663_PENALTY=5.624538
PRIMER_LEFT_663_PENALTY=2.303792
PRIMER_RIGHT_663_PENALTY=3.320745
PRIMER_LEFT_663_SEQUENCE=ATGAATGTCCAGAATGGGAAGT
PRIMER_RIGHT_663_SEQUENCE=CTCACCAACTAAGCAGCGGTAG
PRIMER_LEFT_663=332,22
PRIMER_RIGHT_663=562,22
PRIMER_LEFT_663_TM=59.696
PRIMER_RIGHT_663_TM=61.321
PRIMER_LEFT_663_GC_PERCENT=40.909
PRIMER_RIGHT_663_GC_PERCENT=54.545
PRIMER_LEFT_663_SELF_ANY=3.00
PRIMER_RIGHT_663_SELF_ANY=3.00
PRIMER_LEFT_663_SELF_END=1.00
PRIMER_RIGHT_663_SELF_END=3.00
PRIMER_LEFT_663_END_STABILITY=6.4000
PRIMER_RIGHT_663_END_STABILITY=6.9000
PRIMER_PAIR_663_COMPL_ANY=3.00
PRIMER_PAIR_663_COMPL_END=0.00
PRIMER_PAIR_663_PRODUCT_SIZE=231
PRIMER_PAIR_663_PRODUCT_TM=78.4
PRIMER_PAIR_664_PENALTY=5.762404
PRIMER_LEFT_664_PENALTY=4.067900
PRIMER_RIGHT_664_PENALTY=1.694503
PRIMER_LEFT_664_SEQUENCE=TTCCTGACAAGTGCAAATTCTTA
PRIMER_RIGHT_664_SEQUENCE=CCACCAGACATCCGAGTCA
PRIMER_LEFT_664=585,23
PRIMER_RIGHT_664=829,19
PRIMER_LEFT_664_TM=58.932
PRIMER_RIGHT_664_TM=60.695
PRIMER_LEFT_664_GC_PERCENT=34.783
PRIMER_RIGHT_664_GC_PERCENT=57.895
PRIMER_LEFT_664_SELF_ANY=4.00
PRIMER_RIGHT_664_SELF_ANY=5.00
PRIMER_LEFT_664_SELF_END=2.00
PRIMER_RIGHT_664_SELF_END=3.00
PRIMER_LEFT_664_END_STABILITY=6.0000
PRIMER_RIGHT_664_END_STABILITY=6.4000
PRIMER_PAIR_664_COMPL_ANY=4.00
PRIMER_PAIR_664_COMPL_END=1.00
PRIMER_PAIR_664_PRODUCT_SIZE=245
PRIMER_PAIR_664_PRODUCT_TM=78.2
PRIMER_PAIR_665_PENALTY=5.858935
PRIMER_LEFT_665_PENALTY=4.134169
PRIMER_RIGHT_665_PENALTY=1.724766
PRIMER_LEFT_665_SEQUENCE=GTTTGTAAGTGATGCCCTTCTC
PRIMER_RIGHT_665_SEQUENCE=ACTTTCTTCAGCCAGTGGGC
PRIMER_LEFT_665=562,22
PRIMER_RIGHT_665=778,20
PRIMER_LEFT_665_TM=57.866
PRIMER_RIGHT_665_TM=61.725
PRIMER_LEFT_665_GC_PERCENT=45.455
PRIMER_RIGHT_665_GC_PERCENT=55.000
PRIMER_LEFT_665_SELF_ANY=4.00
PRIMER_RIGHT_665_SELF_ANY=5.00
PRIMER_LEFT_665_SELF_END=2.00
PRIMER_RIGHT_665_SELF_END=3.00
PRIMER_LEFT_665_END_STABILITY=6.7000
PRIMER_RIGHT_665_END_STABILITY=11.2000
PRIMER_PAIR_665_COMPL_ANY=4.00
PRIMER_PAIR_665_COMPL_END=2.00
PRIMER_PAIR_665_PRODUCT_SIZE=217
PRIMER_PAIR_665_PRODUCT_TM=77.4
PRIMER_PAIR_666_PENALTY=5.947785
PRIMER_LEFT_666_PENALTY=4.134169
PRIMER_RIGHT_666_PENALTY=1.813616
PRIMER_LEFT_666_SEQUENCE=GAGTTTGTAAGTGATGCCCTTC
PRIMER_RIGHT_666_SEQUENCE=CAGCAGAATCCACATTGTCA
PRIMER_LEFT_666=560,22
PRIMER_RIGHT_666=797,20
PRIMER_LEFT_666_TM=57.866
PRIMER_RIGHT_666_TM=58.186
PRIMER_LEFT_666_GC_PERCENT=45.455
PRIMER_RIGHT_666_GC_PERCENT=45.000
PRIMER_LEFT_666_SELF_ANY=4.00
PRIMER_RIGHT_666_SELF_ANY=5.00
PRIMER_LEFT_666_SELF_END=2.00
PRIMER_RIGHT_666_SELF_END=3.00
PRIMER_LEFT_666_END_STABILITY=8.2000
PRIMER_RIGHT_666_END_STABILITY=6.7000
PRIMER_PAIR_666_COMPL_ANY=3.00
PRIMER_PAIR_666_COMPL_END=1.00
PRIMER_PAIR_666_PRODUCT_SIZE=238
PRIMER_PAIR_666_PRODUCT_TM=77.5
PRIMER_PAIR_667_PENALTY=5.950952
PRIMER_LEFT_667_PENALTY=4.895143
PRIMER_RIGHT_667_PENALTY=1.055809
PRIMER_LEFT_667_SEQUENCE=GGATCCCACTCGCACAGC
PRIMER_RIGHT_667_SEQUENCE=CAGAACATGGCAATCTGGG
PRIMER_LEFT_667=152,18
PRIMER_RIGHT_667=312,19
PRIMER_LEFT_667_TM=62.895
PRIMER_RIGHT_667_TM=60.056
PRIMER_LEFT_667_GC_PERCENT=66.667
PRIMER_RIGHT_667_GC_PERCENT=52.632
PRIMER_LEFT_667_SELF_ANY=6.00
PRIMER_RIGHT_667_SELF_ANY=4.00
PRIMER_LEFT_667_SELF_END=2.00
PRIMER_RIGHT_667_SELF_END=0.00
PRIMER_LEFT_667_END_STABILITY=7.9000
PRIMER_RIGHT_667_END_STABILITY=9.7000
PRIMER_PAIR_667_COMPL_ANY=4.00
PRIMER_PAIR_667_COMPL_END=2.00
PRIMER_PAIR_667_PRODUCT_SIZE=161
PRIMER_PAIR_667_PRODUCT_TM=83.7
PRIMER_PAIR_668_PENALTY=5.988098
PRIMER_LEFT_668_PENALTY=2.578834
PRIMER_RIGHT_668_PENALTY=3.409264
PRIMER_LEFT_668_SEQUENCE=AATGTCCAGAATGGGAAGTG
PRIMER_RIGHT_668_SEQUENCE=ACTAAGCAGCGGTAGGGAA
PRIMER_LEFT_668=335,20
PRIMER_RIGHT_668=555,19
PRIMER_LEFT_668_TM=57.421
PRIMER_RIGHT_668_TM=57.591
PRIMER_LEFT_668_GC_PERCENT=45.000
PRIMER_RIGHT_668_GC_PERCENT=52.632
PRIMER_LEFT_668_SELF_ANY=3.00
PRIMER_RIGHT_668_SELF_ANY=3.00
PRIMER_LEFT_668_SELF_END=0.00
PRIMER_RIGHT_668_SELF_END=0.00
PRIMER_LEFT_668_END_STABILITY=6.7000
PRIMER_RIGHT_668_END_STABILITY=9.7000
PRIMER_PAIR_668_COMPL_ANY=3.00
PRIMER_PAIR_668_COMPL_END=0.00
PRIMER_PAIR_668_PRODUCT_SIZE=221
PRIMER_PAIR_668_PRODUCT_TM=78.3
PRIMER_PAIR_669_PENALTY=6.001378
PRIMER_LEFT_669_PENALTY=4.361392
PRIMER_RIGHT_669_PENALTY=1.639986
PRIMER_LEFT_669_SEQUENCE=TCCGTCAGTTTCCTCGGC
PRIMER_RIGHT_669_SEQUENCE=AGCAGGCCAGCATTACCAT
PRIMER_LEFT_669=47,18
PRIMER_RIGHT_669=285,19
PRIMER_LEFT_669_TM=62.361
PRIMER_RIGHT_669_TM=60.640
PRIMER_LEFT_669_GC_PERCENT=61.111
PRIMER_RIGHT_669_GC_PERCENT=52.632
PRIMER_LEFT_669_SELF_ANY=3.00
PRIMER_RIGHT_669_SELF_ANY=6.00
PRIMER_LEFT_669_SELF_END=2.00
PRIMER_RIGHT_669_SELF_END=2.00
PRIMER_LEFT_669_END_STABILITY=11.4000
PRIMER_RIGHT_669_END_STABILITY=7.8000
PRIMER_PAIR_669_COMPL_ANY=3.00
PRIMER_PAIR_669_COMPL_END=0.00
PRIMER_PAIR_669_PRODUCT_SIZE=239
PRIMER_PAIR_669_PRODUCT_TM=87.2
PRIMER_PAIR_670_PENALTY=6.005950
PRIMER_LEFT_670_PENALTY=4.159919
PRIMER_RIGHT_670_PENALTY=1.846031
PRIMER_LEFT_670_SEQUENCE=AGTTTGTAAGTGATGCCCTTCTC
PRIMER_RIGHT_670_SEQUENCE=CAGCAGAATCCACATTGTCAC
PRIMER_LEFT_670=561,23
PRIMER_RIGHT_670=797,21
PRIMER_LEFT_670_TM=58.840
PRIMER_RIGHT_670_TM=59.154
PRIMER_LEFT_670_GC_PERCENT=43.478
PRIMER_RIGHT_670_GC_PERCENT=47.619
PRIMER_LEFT_670_SELF_ANY=4.00
PRIMER_RIGHT_670_SELF_ANY=5.00
PRIMER_LEFT_670_SELF_END=2.00
PRIMER_RIGHT_670_SELF_END=3.00
PRIMER_LEFT_670_END_STABILITY=6.7000
PRIMER_RIGHT_670_END_STABILITY=6.1000
PRIMER_PAIR_670_COMPL_ANY=4.00
PRIMER_PAIR_670_COMPL_END=0.00
PRIMER_PAIR_670_PRODUCT_SIZE=237
PRIMER_PAIR_670_PRODUCT_TM=77.4
PRIMER_PAIR_671_PENALTY=6.036992
PRIMER_LEFT_671_PENALTY=4.361392
PRIMER_RIGHT_671_PENALTY=1.675600
PRIMER_LEFT_671_SEQUENCE=TCGGGCTCCGTCAGTTTC
PRIMER_RIGHT_671_SEQUENCE=CCATCAGTGGGTACCTCCA
PRIMER_LEFT_671=41,18
PRIMER_RIGHT_671=270,19
PRIMER_LEFT_671_TM=62.361
PRIMER_RIGHT_671_TM=59.324
PRIMER_LEFT_671_GC_PERCENT=61.111
PRIMER_RIGHT_671_GC_PERCENT=57.895
PRIMER_LEFT_671_SELF_ANY=4.00
PRIMER_RIGHT_671_SELF_ANY=6.00
PRIMER_LEFT_671_SELF_END=0.00
PRIMER_RIGHT_671_SELF_END=2.00
PRIMER_LEFT_671_END_STABILITY=6.7000
PRIMER_RIGHT_671_END_STABILITY=8.2000
PRIMER_PAIR_671_COMPL_ANY=2.00
PRIMER_PAIR_671_COMPL_END=0.00
PRIMER_PAIR_671_PRODUCT_SIZE=230
PRIMER_PAIR_671_PRODUCT_TM=87.8
PRIMER_PAIR_672_PENALTY=6.088629
PRIMER_LEFT_672_PENALTY=4.159919
PRIMER_RIGHT_672_PENALTY=1.928711
PRIMER_LEFT_672_SEQUENCE=GAGTTTGTAAGTGATGCCCTTCT
PRIMER_RIGHT_672_SEQUENCE=CTTTCTTCAGCCAGTGGGC
PRIMER_LEFT_672=560,23
PRIMER_RIGHT_672=777,19
PRIMER_LEFT_672_TM=58.840
PRIMER_RIGHT_672_TM=60.929
PRIMER_LEFT_672_GC_PERCENT=43.478
PRIMER_RIGHT_672_GC_PERCENT=57.895
PRIMER_LEFT_672_SELF_ANY=4.00
PRIMER_RIGHT_672_SELF_ANY=5.00
PRIMER_LEFT_672_SELF_END=0.00
PRIMER_RIGHT_672_SELF_END=3.00
PRIMER_LEFT_672_END_STABILITY=6.7000
PRIMER_RIGHT_672_END_STABILITY=11.2000
PRIMER_PAIR_672_COMPL_ANY=4.00
PRIMER_PAIR_672_COMPL_END=3.00
PRIMER_PAIR_672_PRODUCT_SIZE=218
PRIMER_PAIR_672_PRODUCT_TM=77.5
PRIMER_PAIR_673_PENALTY=6.135491
PRIMER_LEFT_673_PENALTY=2.726227
PRIMER_RIGHT_673_PENALTY=3.409264
PRIMER_LEFT_673_SEQUENCE=CACATGAATGTCCAGAATGG
PRIMER_RIGHT_673_SEQUENCE=AACTAAGCAGCGGTAGGGA
PRIMER_LEFT_673=329,20
PRIMER_RIGHT_673=556,19
PRIMER_LEFT_673_TM=57.274
PRIMER_RIGHT_673_TM=57.591
PRIMER_LEFT_673_GC_PERCENT=45.000
PRIMER_RIGHT_673_GC_PERCENT=52.632
PRIMER_LEFT_673_SELF_ANY=6.00
PRIMER_RIGHT_673_SELF_ANY=3.00
PRIMER_LEFT_673_SELF_END=3.00
PRIMER_RIGHT_673_SELF_END=0.00
PRIMER_LEFT_673_END_STABILITY=8.4000
PRIMER_RIGHT_673_END_STABILITY=9.4000
PRIMER_PAIR_673_COMPL_ANY=3.00
PRIMER_PAIR_673_COMPL_END=0.00
PRIMER_PAIR_673_PRODUCT_SIZE=228
PRIMER_PAIR_673_PRODUCT_TM=78.3
PRIMER_PAIR_674_PENALTY=6.135493
PRIMER_LEFT_674_PENALTY=4.291945
PRIMER_RIGHT_674_PENALTY=1.843548
PRIMER_LEFT_674_SEQUENCE=TTCCTGACAAGTGCAAATTCTTAC
PRIMER_RIGHT_674_SEQUENCE=CAGACATCCGAGTCATCCTC
PRIMER_LEFT_674=585,24
PRIMER_RIGHT_674=825,20
PRIMER_LEFT_674_TM=59.708
PRIMER_RIGHT_674_TM=58.156
PRIMER_LEFT_674_GC_PERCENT=37.500
PRIMER_RIGHT_674_GC_PERCENT=55.000
PRIMER_LEFT_674_SELF_ANY=4.00
PRIMER_RIGHT_674_SELF_ANY=5.00
PRIMER_LEFT_674_SELF_END=2.00
PRIMER_RIGHT_674_SELF_END=2.00
PRIMER_LEFT_674_END_STABILITY=5.7000
PRIMER_RIGHT_674_END_STABILITY=7.9000
PRIMER_PAIR_674_COMPL_ANY=4.00
PRIMER_PAIR_674_COMPL_END=0.00
PRIMER_PAIR_674_PRODUCT_SIZE=241
PRIMER_PAIR_674_PRODUCT_TM=78.0
PRIMER_PAIR_675_PENALTY=6.154322
PRIMER_LEFT_675_PENALTY=2.740545
PRIMER_RIGHT_675_PENALTY=3.413777
PRIMER_LEFT_675_SEQUENCE=GATCCATCAGGGACCAAAA
PRIMER_RIGHT_675_SEQUENCE=ACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_675=362,19
PRIMER_RIGHT_675=567,22
PRIMER_LEFT_675_TM=58.259
PRIMER_RIGHT_675_TM=58.586
PRIMER_LEFT_675_GC_PERCENT=47.368
PRIMER_RIGHT_675_GC_PERCENT=45.455
PRIMER_LEFT_675_SELF_ANY=5.00
PRIMER_RIGHT_675_SELF_ANY=3.00
PRIMER_LEFT_675_SELF_END=0.00
PRIMER_RIGHT_675_SELF_END=3.00
PRIMER_LEFT_675_END_STABILITY=7.6000
PRIMER_RIGHT_675_END_STABILITY=9.7000
PRIMER_PAIR_675_COMPL_ANY=2.00
PRIMER_PAIR_675_COMPL_END=0.00
PRIMER_PAIR_675_PRODUCT_SIZE=206
PRIMER_PAIR_675_PRODUCT_TM=78.3
PRIMER_PAIR_676_PENALTY=6.162464
PRIMER_LEFT_676_PENALTY=2.740545
PRIMER_RIGHT_676_PENALTY=3.421919
PRIMER_LEFT_676_SEQUENCE=GAATGGGAAGTGGGATTCA
PRIMER_RIGHT_676_SEQUENCE=AAGGGCATCACTTACAAACTCAC
PRIMER_LEFT_676=343,19
PRIMER_RIGHT_676=580,23
PRIMER_LEFT_676_TM=58.259
PRIMER_RIGHT_676_TM=59.578
PRIMER_LEFT_676_GC_PERCENT=47.368
PRIMER_RIGHT_676_GC_PERCENT=43.478
PRIMER_LEFT_676_SELF_ANY=4.00
PRIMER_RIGHT_676_SELF_ANY=4.00
PRIMER_LEFT_676_SELF_END=1.00
PRIMER_RIGHT_676_SELF_END=0.00
PRIMER_LEFT_676_END_STABILITY=6.9000
PRIMER_RIGHT_676_END_STABILITY=6.4000
PRIMER_PAIR_676_COMPL_ANY=5.00
PRIMER_PAIR_676_COMPL_END=2.00
PRIMER_PAIR_676_PRODUCT_SIZE=238
PRIMER_PAIR_676_PRODUCT_TM=78.4
PRIMER_PAIR_677_PENALTY=6.219376
PRIMER_LEFT_677_PENALTY=4.291945
PRIMER_RIGHT_677_PENALTY=1.927431
PRIMER_LEFT_677_SEQUENCE=GTTCCTGACAAGTGCAAATTCTTA
PRIMER_RIGHT_677_SEQUENCE=ACCAGACATCCGAGTCATCCT
PRIMER_LEFT_677=584,24
PRIMER_RIGHT_677=827,21
PRIMER_LEFT_677_TM=59.708
PRIMER_RIGHT_677_TM=60.927
PRIMER_LEFT_677_GC_PERCENT=37.500
PRIMER_RIGHT_677_GC_PERCENT=52.381
PRIMER_LEFT_677_SELF_ANY=4.00
PRIMER_RIGHT_677_SELF_ANY=5.00
PRIMER_LEFT_677_SELF_END=2.00
PRIMER_RIGHT_677_SELF_END=1.00
PRIMER_LEFT_677_END_STABILITY=6.0000
PRIMER_RIGHT_677_END_STABILITY=7.8000
PRIMER_PAIR_677_COMPL_ANY=4.00
PRIMER_PAIR_677_COMPL_END=1.00
PRIMER_PAIR_677_PRODUCT_SIZE=244
PRIMER_PAIR_677_PRODUCT_TM=78.1
PRIMER_PAIR_678_PENALTY=6.239278
PRIMER_LEFT_678_PENALTY=2.761803
PRIMER_RIGHT_678_PENALTY=3.477475
PRIMER_LEFT_678_SEQUENCE=TCAGGGACCAAAACCTGCAT
PRIMER_RIGHT_678_SEQUENCE=CACCAACTAAGCAGCGGTA
PRIMER_LEFT_678=368,20
PRIMER_RIGHT_678=560,19
PRIMER_LEFT_678_TM=62.762
PRIMER_RIGHT_678_TM=57.523
PRIMER_LEFT_678_GC_PERCENT=50.000
PRIMER_RIGHT_678_GC_PERCENT=52.632
PRIMER_LEFT_678_SELF_ANY=4.00
PRIMER_RIGHT_678_SELF_ANY=3.00
PRIMER_LEFT_678_SELF_END=2.00
PRIMER_RIGHT_678_SELF_END=2.00
PRIMER_LEFT_678_END_STABILITY=8.4000
PRIMER_RIGHT_678_END_STABILITY=8.9000
PRIMER_PAIR_678_COMPL_ANY=4.00
PRIMER_PAIR_678_COMPL_END=2.00
PRIMER_PAIR_678_PRODUCT_SIZE=193
PRIMER_PAIR_678_PRODUCT_TM=78.5
PRIMER_PAIR_679_PENALTY=6.248071
PRIMER_LEFT_679_PENALTY=2.761803
PRIMER_RIGHT_679_PENALTY=3.486268
PRIMER_LEFT_679_SEQUENCE=CATCAGGGACCAAAACCTGC
PRIMER_RIGHT_679_SEQUENCE=TCACCAACTAAGCAGCGG
PRIMER_LEFT_679=366,20
PRIMER_RIGHT_679=561,18
PRIMER_LEFT_679_TM=62.762
PRIMER_RIGHT_679_TM=58.514
PRIMER_LEFT_679_GC_PERCENT=55.000
PRIMER_RIGHT_679_GC_PERCENT=55.556
PRIMER_LEFT_679_SELF_ANY=4.00
PRIMER_RIGHT_679_SELF_ANY=3.00
PRIMER_LEFT_679_SELF_END=2.00
PRIMER_RIGHT_679_SELF_END=2.00
PRIMER_LEFT_679_END_STABILITY=9.7000
PRIMER_RIGHT_679_END_STABILITY=11.4000
PRIMER_PAIR_679_COMPL_ANY=4.00
PRIMER_PAIR_679_COMPL_END=2.00
PRIMER_PAIR_679_PRODUCT_SIZE=196
PRIMER_PAIR_679_PRODUCT_TM=78.4
PRIMER_PAIR_680_PENALTY=6.251530
PRIMER_LEFT_680_PENALTY=4.284304
PRIMER_RIGHT_680_PENALTY=1.967225
PRIMER_LEFT_680_SEQUENCE=GATGCCCTTCTCGTTCCT
PRIMER_RIGHT_680_SEQUENCE=CATCCGAGTCATCCTCCTCC
PRIMER_LEFT_680=572,18
PRIMER_RIGHT_680=821,20
PRIMER_LEFT_680_TM=57.716
PRIMER_RIGHT_680_TM=61.967
PRIMER_LEFT_680_GC_PERCENT=55.556
PRIMER_RIGHT_680_GC_PERCENT=60.000
PRIMER_LEFT_680_SELF_ANY=2.00
PRIMER_RIGHT_680_SELF_ANY=3.00
PRIMER_LEFT_680_SELF_END=0.00
PRIMER_RIGHT_680_SELF_END=0.00
PRIMER_LEFT_680_END_STABILITY=8.2000
PRIMER_RIGHT_680_END_STABILITY=9.4000
PRIMER_PAIR_680_COMPL_ANY=4.00
PRIMER_PAIR_680_COMPL_END=0.00
PRIMER_PAIR_680_PRODUCT_SIZE=250
PRIMER_PAIR_680_PRODUCT_TM=78.3
PRIMER_PAIR_681_PENALTY=6.327733
PRIMER_LEFT_681_PENALTY=2.831979
PRIMER_RIGHT_681_PENALTY=3.495754
PRIMER_LEFT_681_SEQUENCE=ATCCATCAGGGACCAAAACCT
PRIMER_RIGHT_681_SEQUENCE=CGAGAAGGGCATCACTTACAAA
PRIMER_LEFT_681=363,21
PRIMER_RIGHT_681=584,22
PRIMER_LEFT_681_TM=61.832
PRIMER_RIGHT_681_TM=61.496
PRIMER_LEFT_681_GC_PERCENT=47.619
PRIMER_RIGHT_681_GC_PERCENT=45.455
PRIMER_LEFT_681_SELF_ANY=5.00
PRIMER_RIGHT_681_SELF_ANY=4.00
PRIMER_LEFT_681_SELF_END=0.00
PRIMER_RIGHT_681_SELF_END=1.00
PRIMER_LEFT_681_END_STABILITY=7.9000
PRIMER_RIGHT_681_END_STABILITY=7.0000
PRIMER_PAIR_681_COMPL_ANY=3.00
PRIMER_PAIR_681_COMPL_END=1.00
PRIMER_PAIR_681_PRODUCT_SIZE=222
PRIMER_PAIR_681_PRODUCT_TM=78.4
PRIMER_PAIR_682_PENALTY=6.436450
PRIMER_LEFT_682_PENALTY=4.426754
PRIMER_RIGHT_682_PENALTY=2.009696
PRIMER_LEFT_682_SEQUENCE=TCCTGACAAGTGCAAATTCTTA
PRIMER_RIGHT_682_SEQUENCE=ATCCGAGTCATCCTCCTCC
PRIMER_LEFT_682=586,22
PRIMER_RIGHT_682=820,19
PRIMER_LEFT_682_TM=57.573
PRIMER_RIGHT_682_TM=58.990
PRIMER_LEFT_682_GC_PERCENT=36.364
PRIMER_RIGHT_682_GC_PERCENT=57.895
PRIMER_LEFT_682_SELF_ANY=4.00
PRIMER_RIGHT_682_SELF_ANY=3.00
PRIMER_LEFT_682_SELF_END=2.00
PRIMER_RIGHT_682_SELF_END=0.00
PRIMER_LEFT_682_END_STABILITY=6.0000
PRIMER_RIGHT_682_END_STABILITY=9.4000
PRIMER_PAIR_682_COMPL_ANY=4.00
PRIMER_PAIR_682_COMPL_END=0.00
PRIMER_PAIR_682_PRODUCT_SIZE=235
PRIMER_PAIR_682_PRODUCT_TM=77.9
PRIMER_PAIR_683_PENALTY=6.579028
PRIMER_LEFT_683_PENALTY=4.560670
PRIMER_RIGHT_683_PENALTY=2.018358
PRIMER_LEFT_683_SEQUENCE=TCCTGACAAGTGCAAATTCTTAC
PRIMER_RIGHT_683_SEQUENCE=GACATCCGAGTCATCCTCCTC
PRIMER_LEFT_683=586,23
PRIMER_RIGHT_683=823,21
PRIMER_LEFT_683_TM=58.439
PRIMER_RIGHT_683_TM=61.018
PRIMER_LEFT_683_GC_PERCENT=39.130
PRIMER_RIGHT_683_GC_PERCENT=57.143
PRIMER_LEFT_683_SELF_ANY=4.00
PRIMER_RIGHT_683_SELF_ANY=5.00
PRIMER_LEFT_683_SELF_END=2.00
PRIMER_RIGHT_683_SELF_END=0.00
PRIMER_LEFT_683_END_STABILITY=5.7000
PRIMER_RIGHT_683_END_STABILITY=7.9000
PRIMER_PAIR_683_COMPL_ANY=4.00
PRIMER_PAIR_683_COMPL_END=0.00
PRIMER_PAIR_683_PRODUCT_SIZE=238
PRIMER_PAIR_683_PRODUCT_TM=78.1
PRIMER_PAIR_684_PENALTY=6.604277
PRIMER_LEFT_684_PENALTY=4.521959
PRIMER_RIGHT_684_PENALTY=2.082318
PRIMER_LEFT_684_SEQUENCE=GAGTTTGTAAGTGATGCCCTTCTC
PRIMER_RIGHT_684_SEQUENCE=TTTCTTCAGCCAGTGGGC
PRIMER_LEFT_684=560,24
PRIMER_RIGHT_684=776,18
PRIMER_LEFT_684_TM=60.522
PRIMER_RIGHT_684_TM=59.918
PRIMER_LEFT_684_GC_PERCENT=45.833
PRIMER_RIGHT_684_GC_PERCENT=55.556
PRIMER_LEFT_684_SELF_ANY=4.00
PRIMER_RIGHT_684_SELF_ANY=5.00
PRIMER_LEFT_684_SELF_END=2.00
PRIMER_RIGHT_684_SELF_END=3.00
PRIMER_LEFT_684_END_STABILITY=6.7000
PRIMER_RIGHT_684_END_STABILITY=11.2000
PRIMER_PAIR_684_COMPL_ANY=4.00
PRIMER_PAIR_684_COMPL_END=2.00
PRIMER_PAIR_684_PRODUCT_SIZE=217
PRIMER_PAIR_684_PRODUCT_TM=77.4
PRIMER_PAIR_685_PENALTY=6.605800
PRIMER_LEFT_685_PENALTY=3.077974
PRIMER_RIGHT_685_PENALTY=3.527826
PRIMER_LEFT_685_SEQUENCE=GCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_685_SEQUENCE=GAAGGGCATCACTTACAAACTCA
PRIMER_LEFT_685=417,22
PRIMER_RIGHT_685=581,23
PRIMER_LEFT_685_TM=58.922
PRIMER_RIGHT_685_TM=60.528
PRIMER_LEFT_685_GC_PERCENT=50.000
PRIMER_RIGHT_685_GC_PERCENT=43.478
PRIMER_LEFT_685_SELF_ANY=4.00
PRIMER_RIGHT_685_SELF_ANY=4.00
PRIMER_LEFT_685_SELF_END=1.00
PRIMER_RIGHT_685_SELF_END=1.00
PRIMER_LEFT_685_END_STABILITY=6.4000
PRIMER_RIGHT_685_END_STABILITY=6.4000
PRIMER_PAIR_685_COMPL_ANY=5.00
PRIMER_PAIR_685_COMPL_END=2.00
PRIMER_PAIR_685_PRODUCT_SIZE=165
PRIMER_PAIR_685_PRODUCT_TM=78.1
PRIMER_PAIR_686_PENALTY=6.675183
PRIMER_LEFT_686_PENALTY=4.414025
PRIMER_RIGHT_686_PENALTY=2.261158
PRIMER_LEFT_686_SEQUENCE=TTTCCTCGGCAGCGGTAG
PRIMER_RIGHT_686_SEQUENCE=CAGTGGGTACCTCCAGCG
PRIMER_LEFT_686=55,18
PRIMER_RIGHT_686=266,18
PRIMER_LEFT_686_TM=62.414
PRIMER_RIGHT_686_TM=60.261
PRIMER_LEFT_686_GC_PERCENT=61.111
PRIMER_RIGHT_686_GC_PERCENT=66.667
PRIMER_LEFT_686_SELF_ANY=4.00
PRIMER_RIGHT_686_SELF_ANY=6.00
PRIMER_LEFT_686_SELF_END=3.00
PRIMER_RIGHT_686_SELF_END=2.00
PRIMER_LEFT_686_END_STABILITY=6.9000
PRIMER_RIGHT_686_END_STABILITY=10.2000
PRIMER_PAIR_686_COMPL_ANY=4.00
PRIMER_PAIR_686_COMPL_END=2.00
PRIMER_PAIR_686_PRODUCT_SIZE=212
PRIMER_PAIR_686_PRODUCT_TM=87.8
PRIMER_PAIR_687_PENALTY=6.718648
PRIMER_LEFT_687_PENALTY=4.632309
PRIMER_RIGHT_687_PENALTY=2.086339
PRIMER_LEFT_687_SEQUENCE=GGTGAGTTTGTAAGTGATGCCCT
PRIMER_RIGHT_687_SEQUENCE=CATTGTCACTTTCTTCAGCCAG
PRIMER_LEFT_687=557,23
PRIMER_RIGHT_687=785,22
PRIMER_LEFT_687_TM=61.632
PRIMER_RIGHT_687_TM=59.914
PRIMER_LEFT_687_GC_PERCENT=47.826
PRIMER_RIGHT_687_GC_PERCENT=45.455
PRIMER_LEFT_687_SELF_ANY=2.00
PRIMER_RIGHT_687_SELF_ANY=3.00
PRIMER_LEFT_687_SELF_END=2.00
PRIMER_RIGHT_687_SELF_END=2.00
PRIMER_LEFT_687_END_STABILITY=10.9000
PRIMER_RIGHT_687_END_STABILITY=9.7000
PRIMER_PAIR_687_COMPL_ANY=6.00
PRIMER_PAIR_687_COMPL_END=2.00
PRIMER_PAIR_687_PRODUCT_SIZE=229
PRIMER_PAIR_687_PRODUCT_TM=77.5
PRIMER_PAIR_688_PENALTY=6.773585
PRIMER_LEFT_688_PENALTY=4.685119
PRIMER_RIGHT_688_PENALTY=2.088466
PRIMER_LEFT_688_SEQUENCE=TAAGTGATGCCCTTCTCGTTCC
PRIMER_RIGHT_688_SEQUENCE=CATTGTCACTTTCTTCAGCCA
PRIMER_LEFT_688=567,22
PRIMER_RIGHT_688=785,21
PRIMER_LEFT_688_TM=62.685
PRIMER_RIGHT_688_TM=58.912
PRIMER_LEFT_688_GC_PERCENT=50.000
PRIMER_RIGHT_688_GC_PERCENT=42.857
PRIMER_LEFT_688_SELF_ANY=4.00
PRIMER_RIGHT_688_SELF_ANY=3.00
PRIMER_LEFT_688_SELF_END=0.00
PRIMER_RIGHT_688_SELF_END=0.00
PRIMER_LEFT_688_END_STABILITY=7.9000
PRIMER_RIGHT_688_END_STABILITY=9.7000
PRIMER_PAIR_688_COMPL_ANY=6.00
PRIMER_PAIR_688_COMPL_END=0.00
PRIMER_PAIR_688_PRODUCT_SIZE=219
PRIMER_PAIR_688_PRODUCT_TM=77.4
PRIMER_PAIR_689_PENALTY=6.808167
PRIMER_LEFT_689_PENALTY=3.152169
PRIMER_RIGHT_689_PENALTY=3.655999
PRIMER_LEFT_689_SEQUENCE=CAGATCCATCAGGGACCAAAA
PRIMER_RIGHT_689_SEQUENCE=AGGGCATCACTTACAAACTCA
PRIMER_LEFT_689=360,21
PRIMER_RIGHT_689=579,21
PRIMER_LEFT_689_TM=62.152
PRIMER_RIGHT_689_TM=57.344
PRIMER_LEFT_689_GC_PERCENT=47.619
PRIMER_RIGHT_689_GC_PERCENT=42.857
PRIMER_LEFT_689_SELF_ANY=5.00
PRIMER_RIGHT_689_SELF_ANY=2.00
PRIMER_LEFT_689_SELF_END=0.00
PRIMER_RIGHT_689_SELF_END=1.00
PRIMER_LEFT_689_END_STABILITY=7.6000
PRIMER_RIGHT_689_END_STABILITY=6.4000
PRIMER_PAIR_689_COMPL_ANY=3.00
PRIMER_PAIR_689_COMPL_END=0.00
PRIMER_PAIR_689_PRODUCT_SIZE=220
PRIMER_PAIR_689_PRODUCT_TM=78.4
PRIMER_PAIR_690_PENALTY=6.863341
PRIMER_LEFT_690_PENALTY=3.152169
PRIMER_RIGHT_690_PENALTY=3.711172
PRIMER_LEFT_690_SEQUENCE=CAGAATGGGAAGTGGGATTCA
PRIMER_RIGHT_690_SEQUENCE=ACCAACTAAGCAGCGGTAGGG
PRIMER_LEFT_690=341,21
PRIMER_RIGHT_690=559,21
PRIMER_LEFT_690_TM=62.152
PRIMER_RIGHT_690_TM=62.711
PRIMER_LEFT_690_GC_PERCENT=47.619
PRIMER_RIGHT_690_GC_PERCENT=57.143
PRIMER_LEFT_690_SELF_ANY=4.00
PRIMER_RIGHT_690_SELF_ANY=3.00
PRIMER_LEFT_690_SELF_END=1.00
PRIMER_RIGHT_690_SELF_END=0.00
PRIMER_LEFT_690_END_STABILITY=6.9000
PRIMER_RIGHT_690_END_STABILITY=8.7000
PRIMER_PAIR_690_COMPL_ANY=4.00
PRIMER_PAIR_690_COMPL_END=0.00
PRIMER_PAIR_690_PRODUCT_SIZE=219
PRIMER_PAIR_690_PRODUCT_TM=78.5
PRIMER_PAIR_691_PENALTY=6.912901
PRIMER_LEFT_691_PENALTY=3.165686
PRIMER_RIGHT_691_PENALTY=3.747215
PRIMER_LEFT_691_SEQUENCE=AGATCCATCAGGGACCAAA
PRIMER_RIGHT_691_SEQUENCE=AGGGCATCACTTACAAACTCAC
PRIMER_LEFT_691=361,19
PRIMER_RIGHT_691=579,22
PRIMER_LEFT_691_TM=57.834
PRIMER_RIGHT_691_TM=58.253
PRIMER_LEFT_691_GC_PERCENT=47.368
PRIMER_RIGHT_691_GC_PERCENT=45.455
PRIMER_LEFT_691_SELF_ANY=5.00
PRIMER_RIGHT_691_SELF_ANY=2.00
PRIMER_LEFT_691_SELF_END=0.00
PRIMER_RIGHT_691_SELF_END=0.00
PRIMER_LEFT_691_END_STABILITY=8.8000
PRIMER_RIGHT_691_END_STABILITY=6.4000
PRIMER_PAIR_691_COMPL_ANY=3.00
PRIMER_PAIR_691_COMPL_END=0.00
PRIMER_PAIR_691_PRODUCT_SIZE=219
PRIMER_PAIR_691_PRODUCT_TM=78.3
PRIMER_PAIR_692_PENALTY=6.937130
PRIMER_LEFT_692_PENALTY=4.837235
PRIMER_RIGHT_692_PENALTY=2.099895
PRIMER_LEFT_692_SEQUENCE=CCCTTCTCGTTCCTGACA
PRIMER_RIGHT_692_SEQUENCE=CCTCCGCATCAGCAGAAT
PRIMER_LEFT_692=576,18
PRIMER_RIGHT_692=806,18
PRIMER_LEFT_692_TM=57.163
PRIMER_RIGHT_692_TM=59.900
PRIMER_LEFT_692_GC_PERCENT=55.556
PRIMER_RIGHT_692_GC_PERCENT=55.556
PRIMER_LEFT_692_SELF_ANY=3.00
PRIMER_RIGHT_692_SELF_ANY=4.00
PRIMER_LEFT_692_SELF_END=3.00
PRIMER_RIGHT_692_SELF_END=2.00
PRIMER_LEFT_692_END_STABILITY=6.7000
PRIMER_RIGHT_692_END_STABILITY=6.6000
PRIMER_PAIR_692_COMPL_ANY=4.00
PRIMER_PAIR_692_COMPL_END=1.00
PRIMER_PAIR_692_PRODUCT_SIZE=231
PRIMER_PAIR_692_PRODUCT_TM=78.1
PRIMER_PAIR_693_PENALTY=6.956167
PRIMER_LEFT_693_PENALTY=3.165686
PRIMER_RIGHT_693_PENALTY=3.790481
PRIMER_LEFT_693_SEQUENCE=TGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_693_SEQUENCE=CAACTAAGCAGCGGTAGGGAAT
PRIMER_LEFT_693=346,19
PRIMER_RIGHT_693=557,22
PRIMER_LEFT_693_TM=57.834
PRIMER_RIGHT_693_TM=61.790
PRIMER_LEFT_693_GC_PERCENT=47.368
PRIMER_RIGHT_693_GC_PERCENT=50.000
PRIMER_LEFT_693_SELF_ANY=3.00
PRIMER_RIGHT_693_SELF_ANY=3.00
PRIMER_LEFT_693_SELF_END=2.00
PRIMER_RIGHT_693_SELF_END=2.00
PRIMER_LEFT_693_END_STABILITY=6.6000
PRIMER_RIGHT_693_END_STABILITY=8.1000
PRIMER_PAIR_693_COMPL_ANY=4.00
PRIMER_PAIR_693_COMPL_END=2.00
PRIMER_PAIR_693_PRODUCT_SIZE=212
PRIMER_PAIR_693_PRODUCT_TM=78.5
PRIMER_PAIR_694_PENALTY=6.979428
PRIMER_LEFT_694_PENALTY=3.165686
PRIMER_RIGHT_694_PENALTY=3.813742
PRIMER_LEFT_694_SEQUENCE=ATGGGAAGTGGGATTCAGA
PRIMER_RIGHT_694_SEQUENCE=GGCATCACTTACAAACTCACC
PRIMER_LEFT_694=345,19
PRIMER_RIGHT_694=577,21
PRIMER_LEFT_694_TM=57.834
PRIMER_RIGHT_694_TM=57.186
PRIMER_LEFT_694_GC_PERCENT=47.368
PRIMER_RIGHT_694_GC_PERCENT=47.619
PRIMER_LEFT_694_SELF_ANY=3.00
PRIMER_RIGHT_694_SELF_ANY=2.00
PRIMER_LEFT_694_SELF_END=3.00
PRIMER_RIGHT_694_SELF_END=0.00
PRIMER_LEFT_694_END_STABILITY=6.7000
PRIMER_RIGHT_694_END_STABILITY=7.9000
PRIMER_PAIR_694_COMPL_ANY=5.00
PRIMER_PAIR_694_COMPL_END=1.00
PRIMER_PAIR_694_PRODUCT_SIZE=233
PRIMER_PAIR_694_PRODUCT_TM=78.4
PRIMER_PAIR_695_PENALTY=7.036440
PRIMER_LEFT_695_PENALTY=3.222698
PRIMER_RIGHT_695_PENALTY=3.813742
PRIMER_LEFT_695_SEQUENCE=GAAGTGGGATTCAGATCCATCA
PRIMER_RIGHT_695_SEQUENCE=GGGCATCACTTACAAACTCAC
PRIMER_LEFT_695=349,22
PRIMER_RIGHT_695=578,21
PRIMER_LEFT_695_TM=61.223
PRIMER_RIGHT_695_TM=57.186
PRIMER_LEFT_695_GC_PERCENT=45.455
PRIMER_RIGHT_695_GC_PERCENT=47.619
PRIMER_LEFT_695_SELF_ANY=5.00
PRIMER_RIGHT_695_SELF_ANY=2.00
PRIMER_LEFT_695_SELF_END=2.00
PRIMER_RIGHT_695_SELF_END=0.00
PRIMER_LEFT_695_END_STABILITY=6.9000
PRIMER_RIGHT_695_END_STABILITY=6.4000
PRIMER_PAIR_695_COMPL_ANY=5.00
PRIMER_PAIR_695_COMPL_END=0.00
PRIMER_PAIR_695_PRODUCT_SIZE=230
PRIMER_PAIR_695_PRODUCT_TM=78.5
PRIMER_PAIR_696_PENALTY=7.047570
PRIMER_LEFT_696_PENALTY=5.028844
PRIMER_RIGHT_696_PENALTY=2.018726
PRIMER_LEFT_696_SEQUENCE=TCCTGACAAGTGCAAATTCTTACA
PRIMER_RIGHT_696_SEQUENCE=CCAGACATCCGAGTCATCC
PRIMER_LEFT_696=586,24
PRIMER_RIGHT_696=826,19
PRIMER_LEFT_696_TM=61.029
PRIMER_RIGHT_696_TM=58.981
PRIMER_LEFT_696_GC_PERCENT=37.500
PRIMER_RIGHT_696_GC_PERCENT=57.895
PRIMER_LEFT_696_SELF_ANY=4.00
PRIMER_RIGHT_696_SELF_ANY=5.00
PRIMER_LEFT_696_SELF_END=3.00
PRIMER_RIGHT_696_SELF_END=0.00
PRIMER_LEFT_696_END_STABILITY=6.0000
PRIMER_RIGHT_696_END_STABILITY=8.1000
PRIMER_PAIR_696_COMPL_ANY=4.00
PRIMER_PAIR_696_COMPL_END=0.00
PRIMER_PAIR_696_PRODUCT_SIZE=241
PRIMER_PAIR_696_PRODUCT_TM=78.2
PRIMER_PAIR_697_PENALTY=7.102820
PRIMER_LEFT_697_PENALTY=3.228511
PRIMER_RIGHT_697_PENALTY=3.874309
PRIMER_LEFT_697_SEQUENCE=GGATTCAGATCCATCAGGG
PRIMER_RIGHT_697_SEQUENCE=CAGCGGTAGGGAATCACA
PRIMER_LEFT_697=355,19
PRIMER_RIGHT_697=549,18
PRIMER_LEFT_697_TM=57.771
PRIMER_RIGHT_697_TM=58.126
PRIMER_LEFT_697_GC_PERCENT=52.632
PRIMER_RIGHT_697_GC_PERCENT=55.556
PRIMER_LEFT_697_SELF_ANY=5.00
PRIMER_RIGHT_697_SELF_ANY=3.00
PRIMER_LEFT_697_SELF_END=3.00
PRIMER_RIGHT_697_SELF_END=1.00
PRIMER_LEFT_697_END_STABILITY=9.7000
PRIMER_RIGHT_697_END_STABILITY=6.7000
PRIMER_PAIR_697_COMPL_ANY=5.00
PRIMER_PAIR_697_COMPL_END=0.00
PRIMER_PAIR_697_PRODUCT_SIZE=195
PRIMER_PAIR_697_PRODUCT_TM=78.5
PRIMER_PAIR_698_PENALTY=7.118286
PRIMER_LEFT_698_PENALTY=4.939568
PRIMER_RIGHT_698_PENALTY=2.178718
PRIMER_LEFT_698_SEQUENCE=AGTTTGTAAGTGATGCCCTTCT
PRIMER_RIGHT_698_SEQUENCE=CAGCAGAATCCACATTGTCACT
PRIMER_LEFT_698=561,22
PRIMER_RIGHT_698=797,22
PRIMER_LEFT_698_TM=57.060
PRIMER_RIGHT_698_TM=60.179
PRIMER_LEFT_698_GC_PERCENT=40.909
PRIMER_RIGHT_698_GC_PERCENT=45.455
PRIMER_LEFT_698_SELF_ANY=4.00
PRIMER_RIGHT_698_SELF_ANY=5.00
PRIMER_LEFT_698_SELF_END=0.00
PRIMER_RIGHT_698_SELF_END=3.00
PRIMER_LEFT_698_END_STABILITY=6.7000
PRIMER_RIGHT_698_END_STABILITY=6.4000
PRIMER_PAIR_698_COMPL_ANY=5.00
PRIMER_PAIR_698_COMPL_END=0.00
PRIMER_PAIR_698_PRODUCT_SIZE=237
PRIMER_PAIR_698_PRODUCT_TM=77.4
PRIMER_PAIR_699_PENALTY=7.149466
PRIMER_LEFT_699_PENALTY=3.228511
PRIMER_RIGHT_699_PENALTY=3.920955
PRIMER_LEFT_699_SEQUENCE=GGGATTCAGATCCATCAGG
PRIMER_RIGHT_699_SEQUENCE=CTAAGCAGCGGTAGGGAAT
PRIMER_LEFT_699=354,19
PRIMER_RIGHT_699=554,19
PRIMER_LEFT_699_TM=57.771
PRIMER_RIGHT_699_TM=57.079
PRIMER_LEFT_699_GC_PERCENT=52.632
PRIMER_RIGHT_699_GC_PERCENT=52.632
PRIMER_LEFT_699_SELF_ANY=5.00
PRIMER_RIGHT_699_SELF_ANY=3.00
PRIMER_LEFT_699_SELF_END=2.00
PRIMER_RIGHT_699_SELF_END=2.00
PRIMER_LEFT_699_END_STABILITY=8.2000
PRIMER_RIGHT_699_END_STABILITY=8.1000
PRIMER_PAIR_699_COMPL_ANY=4.00
PRIMER_PAIR_699_COMPL_END=0.00
PRIMER_PAIR_699_PRODUCT_SIZE=201
PRIMER_PAIR_699_PRODUCT_TM=78.5
PRIMER_PAIR_700_PENALTY=7.273914
PRIMER_LEFT_700_PENALTY=5.707913
PRIMER_RIGHT_700_PENALTY=1.566001
PRIMER_LEFT_700_SEQUENCE=AAACTCATCTTCACTGGCACACC
PRIMER_RIGHT_700_SEQUENCE=TAGTCTGTGTCTGCTCCGCC
PRIMER_LEFT_700=633,23
PRIMER_RIGHT_700=849,20
PRIMER_LEFT_700_TM=62.708
PRIMER_RIGHT_700_TM=61.566
PRIMER_LEFT_700_GC_PERCENT=47.826
PRIMER_RIGHT_700_GC_PERCENT=60.000
PRIMER_LEFT_700_SELF_ANY=3.00
PRIMER_RIGHT_700_SELF_ANY=3.00
PRIMER_LEFT_700_SELF_END=0.00
PRIMER_RIGHT_700_SELF_END=1.00
PRIMER_LEFT_700_END_STABILITY=7.6000
PRIMER_RIGHT_700_END_STABILITY=12.9000
PRIMER_PAIR_700_COMPL_ANY=4.00
PRIMER_PAIR_700_COMPL_END=2.00
PRIMER_PAIR_700_PRODUCT_SIZE=217
PRIMER_PAIR_700_PRODUCT_TM=78.7
PRIMER_PAIR_701_PENALTY=7.380842
PRIMER_LEFT_701_PENALTY=5.190656
PRIMER_RIGHT_701_PENALTY=2.190186
PRIMER_LEFT_701_SEQUENCE=TTGTAAGTGATGCCCTTCTCGTT
PRIMER_RIGHT_701_SEQUENCE=CGCATCAGCAGAATCCACAT
PRIMER_LEFT_701=564,23
PRIMER_RIGHT_701=802,20
PRIMER_LEFT_701_TM=62.191
PRIMER_RIGHT_701_TM=62.190
PRIMER_LEFT_701_GC_PERCENT=43.478
PRIMER_RIGHT_701_GC_PERCENT=50.000
PRIMER_LEFT_701_SELF_ANY=4.00
PRIMER_RIGHT_701_SELF_ANY=4.00
PRIMER_LEFT_701_SELF_END=2.00
PRIMER_RIGHT_701_SELF_END=2.00
PRIMER_LEFT_701_END_STABILITY=8.4000
PRIMER_RIGHT_701_END_STABILITY=6.6000
PRIMER_PAIR_701_COMPL_ANY=6.00
PRIMER_PAIR_701_COMPL_END=1.00
PRIMER_PAIR_701_PRODUCT_SIZE=239
PRIMER_PAIR_701_PRODUCT_TM=77.6
PRIMER_PAIR_702_PENALTY=7.385193
PRIMER_LEFT_702_PENALTY=5.253665
PRIMER_RIGHT_702_PENALTY=2.131528
PRIMER_LEFT_702_SEQUENCE=GTGAGTTTGTAAGTGATGCCCTTC
PRIMER_RIGHT_702_SEQUENCE=CCAGTGGGCAACACACAA
PRIMER_LEFT_702=558,24
PRIMER_RIGHT_702=767,18
PRIMER_LEFT_702_TM=61.254
PRIMER_RIGHT_702_TM=60.132
PRIMER_LEFT_702_GC_PERCENT=45.833
PRIMER_RIGHT_702_GC_PERCENT=55.556
PRIMER_LEFT_702_SELF_ANY=4.00
PRIMER_RIGHT_702_SELF_ANY=5.00
PRIMER_LEFT_702_SELF_END=2.00
PRIMER_RIGHT_702_SELF_END=0.00
PRIMER_LEFT_702_END_STABILITY=8.2000
PRIMER_RIGHT_702_END_STABILITY=7.0000
PRIMER_PAIR_702_COMPL_ANY=7.00
PRIMER_PAIR_702_COMPL_END=3.00
PRIMER_PAIR_702_PRODUCT_SIZE=210
PRIMER_PAIR_702_PRODUCT_TM=77.5
PRIMER_PAIR_703_PENALTY=7.410540
PRIMER_LEFT_703_PENALTY=5.190656
PRIMER_RIGHT_703_PENALTY=2.219884
PRIMER_LEFT_703_SEQUENCE=TTTGTAAGTGATGCCCTTCTCGT
PRIMER_RIGHT_703_SEQUENCE=ACATTGTCACTTTCTTCAGCCA
PRIMER_LEFT_703=563,23
PRIMER_RIGHT_703=786,22
PRIMER_LEFT_703_TM=62.191
PRIMER_RIGHT_703_TM=59.780
PRIMER_LEFT_703_GC_PERCENT=43.478
PRIMER_RIGHT_703_GC_PERCENT=40.909
PRIMER_LEFT_703_SELF_ANY=4.00
PRIMER_RIGHT_703_SELF_ANY=5.00
PRIMER_LEFT_703_SELF_END=2.00
PRIMER_RIGHT_703_SELF_END=0.00
PRIMER_LEFT_703_END_STABILITY=8.1000
PRIMER_RIGHT_703_END_STABILITY=9.7000
PRIMER_PAIR_703_COMPL_ANY=6.00
PRIMER_PAIR_703_COMPL_END=1.00
PRIMER_PAIR_703_PRODUCT_SIZE=224
PRIMER_PAIR_703_PRODUCT_TM=77.2
PRIMER_PAIR_704_PENALTY=7.410540
PRIMER_LEFT_704_PENALTY=5.190656
PRIMER_RIGHT_704_PENALTY=2.219884
PRIMER_LEFT_704_SEQUENCE=GTTTGTAAGTGATGCCCTTCTCG
PRIMER_RIGHT_704_SEQUENCE=CACATTGTCACTTTCTTCAGCC
PRIMER_LEFT_704=562,23
PRIMER_RIGHT_704=787,22
PRIMER_LEFT_704_TM=62.191
PRIMER_RIGHT_704_TM=59.780
PRIMER_LEFT_704_GC_PERCENT=47.826
PRIMER_RIGHT_704_GC_PERCENT=45.455
PRIMER_LEFT_704_SELF_ANY=4.00
PRIMER_RIGHT_704_SELF_ANY=5.00
PRIMER_LEFT_704_SELF_END=2.00
PRIMER_RIGHT_704_SELF_END=1.00
PRIMER_LEFT_704_END_STABILITY=8.4000
PRIMER_RIGHT_704_END_STABILITY=9.7000
PRIMER_PAIR_704_COMPL_ANY=6.00
PRIMER_PAIR_704_COMPL_END=1.00
PRIMER_PAIR_704_PRODUCT_SIZE=226
PRIMER_PAIR_704_PRODUCT_TM=77.4
PRIMER_PAIR_705_PENALTY=7.421562
PRIMER_LEFT_705_PENALTY=3.309654
PRIMER_RIGHT_705_PENALTY=4.111908
PRIMER_LEFT_705_SEQUENCE=CCATCAGGGACCAAAACC
PRIMER_RIGHT_705_SEQUENCE=TTACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_705=365,18
PRIMER_RIGHT_705=569,24
PRIMER_LEFT_705_TM=58.690
PRIMER_RIGHT_705_TM=59.888
PRIMER_LEFT_705_GC_PERCENT=55.556
PRIMER_RIGHT_705_GC_PERCENT=41.667
PRIMER_LEFT_705_SELF_ANY=3.00
PRIMER_RIGHT_705_SELF_ANY=3.00
PRIMER_LEFT_705_SELF_END=0.00
PRIMER_RIGHT_705_SELF_END=3.00
PRIMER_LEFT_705_END_STABILITY=8.2000
PRIMER_RIGHT_705_END_STABILITY=9.7000
PRIMER_PAIR_705_COMPL_ANY=2.00
PRIMER_PAIR_705_COMPL_END=0.00
PRIMER_PAIR_705_PRODUCT_SIZE=205
PRIMER_PAIR_705_PRODUCT_TM=78.2
PRIMER_PAIR_706_PENALTY=7.515064
PRIMER_LEFT_706_PENALTY=3.380895
PRIMER_RIGHT_706_PENALTY=4.134169
PRIMER_LEFT_706_SEQUENCE=GTCCAGAATGGGAAGTGGGAT
PRIMER_RIGHT_706_SEQUENCE=GAAGGGCATCACTTACAAACTC
PRIMER_LEFT_706=338,21
PRIMER_RIGHT_706=581,22
PRIMER_LEFT_706_TM=62.381
PRIMER_RIGHT_706_TM=57.866
PRIMER_LEFT_706_GC_PERCENT=52.381
PRIMER_RIGHT_706_GC_PERCENT=45.455
PRIMER_LEFT_706_SELF_ANY=3.00
PRIMER_RIGHT_706_SELF_ANY=4.00
PRIMER_LEFT_706_SELF_END=2.00
PRIMER_RIGHT_706_SELF_END=0.00
PRIMER_LEFT_706_END_STABILITY=9.3000
PRIMER_RIGHT_706_END_STABILITY=6.4000
PRIMER_PAIR_706_COMPL_ANY=5.00
PRIMER_PAIR_706_COMPL_END=2.00
PRIMER_PAIR_706_PRODUCT_SIZE=244
PRIMER_PAIR_706_PRODUCT_TM=78.6
PRIMER_PAIR_707_PENALTY=7.541935
PRIMER_LEFT_707_PENALTY=3.407766
PRIMER_RIGHT_707_PENALTY=4.134169
PRIMER_LEFT_707_SEQUENCE=GAATGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_707_SEQUENCE=GAGAAGGGCATCACTTACAAAC
PRIMER_LEFT_707=343,22
PRIMER_RIGHT_707=583,22
PRIMER_LEFT_707_TM=61.408
PRIMER_RIGHT_707_TM=57.866
PRIMER_LEFT_707_GC_PERCENT=45.455
PRIMER_RIGHT_707_GC_PERCENT=45.455
PRIMER_LEFT_707_SELF_ANY=4.00
PRIMER_RIGHT_707_SELF_ANY=4.00
PRIMER_LEFT_707_SELF_END=2.00
PRIMER_RIGHT_707_SELF_END=0.00
PRIMER_LEFT_707_END_STABILITY=6.6000
PRIMER_RIGHT_707_END_STABILITY=7.0000
PRIMER_PAIR_707_COMPL_ANY=5.00
PRIMER_PAIR_707_COMPL_END=1.00
PRIMER_PAIR_707_PRODUCT_SIZE=241
PRIMER_PAIR_707_PRODUCT_TM=78.5
PRIMER_PAIR_708_PENALTY=7.553264
PRIMER_LEFT_708_PENALTY=5.431239
PRIMER_RIGHT_708_PENALTY=2.122026
PRIMER_LEFT_708_SEQUENCE=GTTCCTGACAAGTGCAAATTCTTAC
PRIMER_RIGHT_708_SEQUENCE=GAGTCATCCTCCTCCGCATC
PRIMER_LEFT_708=584,25
PRIMER_RIGHT_708=816,20
PRIMER_LEFT_708_TM=60.431
PRIMER_RIGHT_708_TM=62.122
PRIMER_LEFT_708_GC_PERCENT=40.000
PRIMER_RIGHT_708_GC_PERCENT=60.000
PRIMER_LEFT_708_SELF_ANY=4.00
PRIMER_RIGHT_708_SELF_ANY=3.00
PRIMER_LEFT_708_SELF_END=2.00
PRIMER_RIGHT_708_SELF_END=0.00
PRIMER_LEFT_708_END_STABILITY=5.7000
PRIMER_RIGHT_708_END_STABILITY=8.1000
PRIMER_PAIR_708_COMPL_ANY=4.00
PRIMER_PAIR_708_COMPL_END=0.00
PRIMER_PAIR_708_PRODUCT_SIZE=233
PRIMER_PAIR_708_PRODUCT_TM=77.9
PRIMER_PAIR_709_PENALTY=7.583554
PRIMER_LEFT_709_PENALTY=5.363671
PRIMER_RIGHT_709_PENALTY=2.219884
PRIMER_LEFT_709_SEQUENCE=TGAGTTTGTAAGTGATGCCCTTCT
PRIMER_RIGHT_709_SEQUENCE=CCACATTGTCACTTTCTTCAGC
PRIMER_LEFT_709=559,24
PRIMER_RIGHT_709=788,22
PRIMER_LEFT_709_TM=61.364
PRIMER_RIGHT_709_TM=59.780
PRIMER_LEFT_709_GC_PERCENT=41.667
PRIMER_RIGHT_709_GC_PERCENT=45.455
PRIMER_LEFT_709_SELF_ANY=4.00
PRIMER_RIGHT_709_SELF_ANY=5.00
PRIMER_LEFT_709_SELF_END=0.00
PRIMER_RIGHT_709_SELF_END=2.00
PRIMER_LEFT_709_END_STABILITY=6.7000
PRIMER_RIGHT_709_END_STABILITY=8.2000
PRIMER_PAIR_709_COMPL_ANY=6.00
PRIMER_PAIR_709_COMPL_END=1.00
PRIMER_PAIR_709_PRODUCT_SIZE=230
PRIMER_PAIR_709_PRODUCT_TM=77.4
PRIMER_PAIR_710_PENALTY=7.663120
PRIMER_LEFT_710_PENALTY=3.496786
PRIMER_RIGHT_710_PENALTY=4.166335
PRIMER_LEFT_710_SEQUENCE=ACATGAATGTCCAGAATGGGAA
PRIMER_RIGHT_710_SEQUENCE=AAGCAGCGGTAGGGAATC
PRIMER_LEFT_710=330,22
PRIMER_RIGHT_710=552,18
PRIMER_LEFT_710_TM=61.497
PRIMER_RIGHT_710_TM=57.834
PRIMER_LEFT_710_GC_PERCENT=40.909
PRIMER_RIGHT_710_GC_PERCENT=55.556
PRIMER_LEFT_710_SELF_ANY=6.00
PRIMER_RIGHT_710_SELF_ANY=3.00
PRIMER_LEFT_710_SELF_END=1.00
PRIMER_RIGHT_710_SELF_END=3.00
PRIMER_LEFT_710_END_STABILITY=9.7000
PRIMER_RIGHT_710_END_STABILITY=6.6000
PRIMER_PAIR_710_COMPL_ANY=3.00
PRIMER_PAIR_710_COMPL_END=1.00
PRIMER_PAIR_710_PRODUCT_SIZE=223
PRIMER_PAIR_710_PRODUCT_TM=78.4
PRIMER_PAIR_711_PENALTY=7.803086
PRIMER_LEFT_711_PENALTY=3.615453
PRIMER_RIGHT_711_PENALTY=4.187633
PRIMER_LEFT_711_SEQUENCE=CATGAATGTCCAGAATGGGAAG
PRIMER_RIGHT_711_SEQUENCE=GCATCACTTACAAACTCACCAA
PRIMER_LEFT_711=331,22
PRIMER_RIGHT_711=576,22
PRIMER_LEFT_711_TM=61.615
PRIMER_RIGHT_711_TM=57.812
PRIMER_LEFT_711_GC_PERCENT=45.455
PRIMER_RIGHT_711_GC_PERCENT=40.909
PRIMER_LEFT_711_SELF_ANY=4.00
PRIMER_RIGHT_711_SELF_ANY=2.00
PRIMER_LEFT_711_SELF_END=0.00
PRIMER_RIGHT_711_SELF_END=0.00
PRIMER_LEFT_711_END_STABILITY=8.2000
PRIMER_RIGHT_711_END_STABILITY=8.2000
PRIMER_PAIR_711_COMPL_ANY=3.00
PRIMER_PAIR_711_COMPL_END=2.00
PRIMER_PAIR_711_PRODUCT_SIZE=246
PRIMER_PAIR_711_PRODUCT_TM=78.3
PRIMER_PAIR_712_PENALTY=7.817035
PRIMER_LEFT_712_PENALTY=3.657116
PRIMER_RIGHT_712_PENALTY=4.159919
PRIMER_LEFT_712_SEQUENCE=AATGGGAAGTGGGATTCAG
PRIMER_RIGHT_712_SEQUENCE=AGAAGGGCATCACTTACAAACTC
PRIMER_LEFT_712=344,19
PRIMER_RIGHT_712=582,23
PRIMER_LEFT_712_TM=57.343
PRIMER_RIGHT_712_TM=58.840
PRIMER_LEFT_712_GC_PERCENT=47.368
PRIMER_RIGHT_712_GC_PERCENT=43.478
PRIMER_LEFT_712_SELF_ANY=3.00
PRIMER_RIGHT_712_SELF_ANY=4.00
PRIMER_LEFT_712_SELF_END=1.00
PRIMER_RIGHT_712_SELF_END=0.00
PRIMER_LEFT_712_END_STABILITY=7.0000
PRIMER_RIGHT_712_END_STABILITY=6.4000
PRIMER_PAIR_712_COMPL_ANY=5.00
PRIMER_PAIR_712_COMPL_END=1.00
PRIMER_PAIR_712_PRODUCT_SIZE=239
PRIMER_PAIR_712_PRODUCT_TM=78.3
PRIMER_PAIR_713_PENALTY=7.817035
PRIMER_LEFT_713_PENALTY=3.657116
PRIMER_RIGHT_713_PENALTY=4.159919
PRIMER_LEFT_713_SEQUENCE=CAGAATGGGAAGTGGGATT
PRIMER_RIGHT_713_SEQUENCE=GAGAAGGGCATCACTTACAAACT
PRIMER_LEFT_713=341,19
PRIMER_RIGHT_713=583,23
PRIMER_LEFT_713_TM=57.343
PRIMER_RIGHT_713_TM=58.840
PRIMER_LEFT_713_GC_PERCENT=47.368
PRIMER_RIGHT_713_GC_PERCENT=43.478
PRIMER_LEFT_713_SELF_ANY=3.00
PRIMER_RIGHT_713_SELF_ANY=4.00
PRIMER_LEFT_713_SELF_END=1.00
PRIMER_RIGHT_713_SELF_END=1.00
PRIMER_LEFT_713_END_STABILITY=8.1000
PRIMER_RIGHT_713_END_STABILITY=6.7000
PRIMER_PAIR_713_COMPL_ANY=5.00
PRIMER_PAIR_713_COMPL_END=1.00
PRIMER_PAIR_713_PRODUCT_SIZE=243
PRIMER_PAIR_713_PRODUCT_TM=78.5
PRIMER_PAIR_714_PENALTY=7.859272
PRIMER_LEFT_714_PENALTY=5.725379
PRIMER_RIGHT_714_PENALTY=2.133893
PRIMER_LEFT_714_SEQUENCE=CGTTCCTGACAAGTGCAAATTCT
PRIMER_RIGHT_714_SEQUENCE=GAGTCATCCTCCTCCGCA
PRIMER_LEFT_714=583,23
PRIMER_RIGHT_714=816,18
PRIMER_LEFT_714_TM=62.725
PRIMER_RIGHT_714_TM=59.866
PRIMER_LEFT_714_GC_PERCENT=43.478
PRIMER_RIGHT_714_GC_PERCENT=61.111
PRIMER_LEFT_714_SELF_ANY=4.00
PRIMER_RIGHT_714_SELF_ANY=3.00
PRIMER_LEFT_714_SELF_END=2.00
PRIMER_RIGHT_714_SELF_END=2.00
PRIMER_LEFT_714_END_STABILITY=6.6000
PRIMER_RIGHT_714_END_STABILITY=11.7000
PRIMER_PAIR_714_COMPL_ANY=4.00
PRIMER_PAIR_714_COMPL_END=1.00
PRIMER_PAIR_714_PRODUCT_SIZE=234
PRIMER_PAIR_714_PRODUCT_TM=78.0
PRIMER_PAIR_715_PENALTY=7.871200
PRIMER_LEFT_715_PENALTY=3.672773
PRIMER_RIGHT_715_PENALTY=4.198427
PRIMER_LEFT_715_SEQUENCE=TGAATGTCCAGAATGGGAA
PRIMER_RIGHT_715_SEQUENCE=CAAACTCACCAACTAAGCAGCG
PRIMER_LEFT_715=333,19
PRIMER_RIGHT_715=566,22
PRIMER_LEFT_715_TM=57.327
PRIMER_RIGHT_715_TM=62.198
PRIMER_LEFT_715_GC_PERCENT=42.105
PRIMER_RIGHT_715_GC_PERCENT=50.000
PRIMER_LEFT_715_SELF_ANY=3.00
PRIMER_RIGHT_715_SELF_ANY=3.00
PRIMER_LEFT_715_SELF_END=1.00
PRIMER_RIGHT_715_SELF_END=2.00
PRIMER_LEFT_715_END_STABILITY=9.7000
PRIMER_RIGHT_715_END_STABILITY=10.2000
PRIMER_PAIR_715_COMPL_ANY=3.00
PRIMER_PAIR_715_COMPL_END=0.00
PRIMER_PAIR_715_PRODUCT_SIZE=234
PRIMER_PAIR_715_PRODUCT_TM=78.4
PRIMER_PAIR_716_PENALTY=7.977121
PRIMER_LEFT_716_PENALTY=5.713033
PRIMER_RIGHT_716_PENALTY=2.264088
PRIMER_LEFT_716_SEQUENCE=TGTAAGTGATGCCCTTCTCGTTC
PRIMER_RIGHT_716_SEQUENCE=TGGGCAACACACAAACTCTA
PRIMER_LEFT_716=565,23
PRIMER_RIGHT_716=763,20
PRIMER_LEFT_716_TM=62.713
PRIMER_RIGHT_716_TM=57.736
PRIMER_LEFT_716_GC_PERCENT=47.826
PRIMER_RIGHT_716_GC_PERCENT=45.000
PRIMER_LEFT_716_SELF_ANY=4.00
PRIMER_RIGHT_716_SELF_ANY=2.00
PRIMER_LEFT_716_SELF_END=2.00
PRIMER_RIGHT_716_SELF_END=2.00
PRIMER_LEFT_716_END_STABILITY=8.4000
PRIMER_RIGHT_716_END_STABILITY=5.7000
PRIMER_PAIR_716_COMPL_ANY=7.00
PRIMER_PAIR_716_COMPL_END=0.00
PRIMER_PAIR_716_PRODUCT_SIZE=199
PRIMER_PAIR_716_PRODUCT_TM=77.3
PRIMER_PAIR_717_PENALTY=8.015748
PRIMER_LEFT_717_PENALTY=3.731444
PRIMER_RIGHT_717_PENALTY=4.284304
PRIMER_LEFT_717_SEQUENCE=TCAGATCCATCAGGGACCAAA
PRIMER_RIGHT_717_SEQUENCE=AGGAACGAGAAGGGCATC
PRIMER_LEFT_717=359,21
PRIMER_RIGHT_717=589,18
PRIMER_LEFT_717_TM=62.731
PRIMER_RIGHT_717_TM=57.716
PRIMER_LEFT_717_GC_PERCENT=47.619
PRIMER_RIGHT_717_GC_PERCENT=55.556
PRIMER_LEFT_717_SELF_ANY=5.00
PRIMER_RIGHT_717_SELF_ANY=2.00
PRIMER_LEFT_717_SELF_END=3.00
PRIMER_RIGHT_717_SELF_END=2.00
PRIMER_LEFT_717_END_STABILITY=8.8000
PRIMER_RIGHT_717_END_STABILITY=8.1000
PRIMER_PAIR_717_COMPL_ANY=3.00
PRIMER_PAIR_717_COMPL_END=0.00
PRIMER_PAIR_717_PRODUCT_SIZE=231
PRIMER_PAIR_717_PRODUCT_TM=78.4
PRIMER_PAIR_718_PENALTY=8.024632
PRIMER_LEFT_718_PENALTY=3.731444
PRIMER_RIGHT_718_PENALTY=4.293188
PRIMER_LEFT_718_SEQUENCE=TTCAGATCCATCAGGGACCAA
PRIMER_RIGHT_718_SEQUENCE=GGCATCACTTACAAACTCACCAA
PRIMER_LEFT_718=358,21
PRIMER_RIGHT_718=577,23
PRIMER_LEFT_718_TM=62.731
PRIMER_RIGHT_718_TM=61.293
PRIMER_LEFT_718_GC_PERCENT=47.619
PRIMER_RIGHT_718_GC_PERCENT=43.478
PRIMER_LEFT_718_SELF_ANY=5.00
PRIMER_RIGHT_718_SELF_ANY=2.00
PRIMER_LEFT_718_SELF_END=1.00
PRIMER_RIGHT_718_SELF_END=0.00
PRIMER_LEFT_718_END_STABILITY=8.2000
PRIMER_RIGHT_718_END_STABILITY=8.2000
PRIMER_PAIR_718_COMPL_ANY=3.00
PRIMER_PAIR_718_COMPL_END=0.00
PRIMER_PAIR_718_PRODUCT_SIZE=220
PRIMER_PAIR_718_PRODUCT_TM=78.2
PRIMER_PAIR_719_PENALTY=8.025488
PRIMER_LEFT_719_PENALTY=3.731877
PRIMER_RIGHT_719_PENALTY=4.293610
PRIMER_LEFT_719_SEQUENCE=GATCCATCAGGGACCAAAACC
PRIMER_RIGHT_719_SEQUENCE=CTAAGCAGCGGTAGGGAATCAC
PRIMER_LEFT_719=362,21
PRIMER_RIGHT_719=554,22
PRIMER_LEFT_719_TM=62.732
PRIMER_RIGHT_719_TM=62.294
PRIMER_LEFT_719_GC_PERCENT=52.381
PRIMER_RIGHT_719_GC_PERCENT=54.545
PRIMER_LEFT_719_SELF_ANY=5.00
PRIMER_RIGHT_719_SELF_ANY=3.00
PRIMER_LEFT_719_SELF_END=0.00
PRIMER_RIGHT_719_SELF_END=3.00
PRIMER_LEFT_719_END_STABILITY=8.2000
PRIMER_RIGHT_719_END_STABILITY=6.3000
PRIMER_PAIR_719_COMPL_ANY=3.00
PRIMER_PAIR_719_COMPL_END=0.00
PRIMER_PAIR_719_PRODUCT_SIZE=193
PRIMER_PAIR_719_PRODUCT_TM=78.5
PRIMER_PAIR_720_PENALTY=8.079760
PRIMER_LEFT_720_PENALTY=5.781967
PRIMER_RIGHT_720_PENALTY=2.297793
PRIMER_LEFT_720_SEQUENCE=TGACAAGTGCAAATTCTTACACCA
PRIMER_RIGHT_720_SEQUENCE=AGTGGGCAACACACAAACTCTA
PRIMER_LEFT_720=589,24
PRIMER_RIGHT_720=765,22
PRIMER_LEFT_720_TM=61.782
PRIMER_RIGHT_720_TM=59.702
PRIMER_LEFT_720_GC_PERCENT=37.500
PRIMER_RIGHT_720_GC_PERCENT=45.455
PRIMER_LEFT_720_SELF_ANY=5.00
PRIMER_RIGHT_720_SELF_ANY=3.00
PRIMER_LEFT_720_SELF_END=1.00
PRIMER_RIGHT_720_SELF_END=2.00
PRIMER_LEFT_720_END_STABILITY=8.2000
PRIMER_RIGHT_720_END_STABILITY=5.7000
PRIMER_PAIR_720_COMPL_ANY=3.00
PRIMER_PAIR_720_COMPL_END=0.00
PRIMER_PAIR_720_PRODUCT_SIZE=177
PRIMER_PAIR_720_PRODUCT_TM=76.9
PRIMER_PAIR_721_PENALTY=8.103842
PRIMER_LEFT_721_PENALTY=3.810232
PRIMER_RIGHT_721_PENALTY=4.293610
PRIMER_LEFT_721_SEQUENCE=CATGAATGTCCAGAATGGG
PRIMER_RIGHT_721_SEQUENCE=ACTAAGCAGCGGTAGGGAATCA
PRIMER_LEFT_721=331,19
PRIMER_RIGHT_721=555,22
PRIMER_LEFT_721_TM=57.190
PRIMER_RIGHT_721_TM=62.294
PRIMER_LEFT_721_GC_PERCENT=47.368
PRIMER_RIGHT_721_GC_PERCENT=50.000
PRIMER_LEFT_721_SELF_ANY=4.00
PRIMER_RIGHT_721_SELF_ANY=3.00
PRIMER_LEFT_721_SELF_END=1.00
PRIMER_RIGHT_721_SELF_END=1.00
PRIMER_LEFT_721_END_STABILITY=9.6000
PRIMER_RIGHT_721_END_STABILITY=6.9000
PRIMER_PAIR_721_COMPL_ANY=3.00
PRIMER_PAIR_721_COMPL_END=0.00
PRIMER_PAIR_721_PRODUCT_SIZE=225
PRIMER_PAIR_721_PRODUCT_TM=78.4
PRIMER_PAIR_722_PENALTY=8.133235
PRIMER_LEFT_722_PENALTY=3.823109
PRIMER_RIGHT_722_PENALTY=4.310125
PRIMER_LEFT_722_SEQUENCE=ATCCATCAGGGACCAAAAC
PRIMER_RIGHT_722_SEQUENCE=GCATCACTTACAAACTCACCAAC
PRIMER_LEFT_722=363,19
PRIMER_RIGHT_722=576,23
PRIMER_LEFT_722_TM=57.177
PRIMER_RIGHT_722_TM=58.690
PRIMER_LEFT_722_GC_PERCENT=47.368
PRIMER_RIGHT_722_GC_PERCENT=43.478
PRIMER_LEFT_722_SELF_ANY=5.00
PRIMER_RIGHT_722_SELF_ANY=2.00
PRIMER_LEFT_722_SELF_END=0.00
PRIMER_RIGHT_722_SELF_END=0.00
PRIMER_LEFT_722_END_STABILITY=7.0000
PRIMER_RIGHT_722_END_STABILITY=8.2000
PRIMER_PAIR_722_COMPL_ANY=3.00
PRIMER_PAIR_722_COMPL_END=0.00
PRIMER_PAIR_722_PRODUCT_SIZE=214
PRIMER_PAIR_722_PRODUCT_TM=78.2
PRIMER_PAIR_723_PENALTY=8.193379
PRIMER_LEFT_723_PENALTY=3.840048
PRIMER_RIGHT_723_PENALTY=4.353331
PRIMER_LEFT_723_SEQUENCE=CCAGAATGGGAAGTGGGATTC
PRIMER_RIGHT_723_SEQUENCE=TACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_723=340,21
PRIMER_RIGHT_723=568,23
PRIMER_LEFT_723_TM=62.840
PRIMER_RIGHT_723_TM=58.647
PRIMER_LEFT_723_GC_PERCENT=52.381
PRIMER_RIGHT_723_GC_PERCENT=43.478
PRIMER_LEFT_723_SELF_ANY=4.00
PRIMER_RIGHT_723_SELF_ANY=3.00
PRIMER_LEFT_723_SELF_END=3.00
PRIMER_RIGHT_723_SELF_END=3.00
PRIMER_LEFT_723_END_STABILITY=6.6000
PRIMER_RIGHT_723_END_STABILITY=9.7000
PRIMER_PAIR_723_COMPL_ANY=4.00
PRIMER_PAIR_723_COMPL_END=1.00
PRIMER_PAIR_723_PRODUCT_SIZE=229
PRIMER_PAIR_723_PRODUCT_TM=78.4
PRIMER_PAIR_724_PENALTY=8.210802
PRIMER_LEFT_724_PENALTY=3.840048
PRIMER_RIGHT_724_PENALTY=4.370754
PRIMER_LEFT_724_SEQUENCE=TCCAGAATGGGAAGTGGGATT
PRIMER_RIGHT_724_SEQUENCE=GCATCACTTACAAACTCACCAACT
PRIMER_LEFT_724=339,21
PRIMER_RIGHT_724=576,24
PRIMER_LEFT_724_TM=62.840
PRIMER_RIGHT_724_TM=59.629
PRIMER_LEFT_724_GC_PERCENT=47.619
PRIMER_RIGHT_724_GC_PERCENT=41.667
PRIMER_LEFT_724_SELF_ANY=3.00
PRIMER_RIGHT_724_SELF_ANY=2.00
PRIMER_LEFT_724_SELF_END=1.00
PRIMER_RIGHT_724_SELF_END=1.00
PRIMER_LEFT_724_END_STABILITY=8.1000
PRIMER_RIGHT_724_END_STABILITY=6.7000
PRIMER_PAIR_724_COMPL_ANY=5.00
PRIMER_PAIR_724_COMPL_END=3.00
PRIMER_PAIR_724_PRODUCT_SIZE=238
PRIMER_PAIR_724_PRODUCT_TM=78.4
PRIMER_PAIR_725_PENALTY=8.291522
PRIMER_LEFT_725_PENALTY=3.864768
PRIMER_RIGHT_725_PENALTY=4.426754
PRIMER_LEFT_725_SEQUENCE=CAAGAAGTCTACCCTGAACTGC
PRIMER_RIGHT_725_SEQUENCE=TAAGAATTTGCACTTGTCAGGA
PRIMER_LEFT_725=419,22
PRIMER_RIGHT_725=607,22
PRIMER_LEFT_725_TM=58.135
PRIMER_RIGHT_725_TM=57.573
PRIMER_LEFT_725_GC_PERCENT=50.000
PRIMER_RIGHT_725_GC_PERCENT=36.364
PRIMER_LEFT_725_SELF_ANY=4.00
PRIMER_RIGHT_725_SELF_ANY=4.00
PRIMER_LEFT_725_SELF_END=2.00
PRIMER_RIGHT_725_SELF_END=2.00
PRIMER_LEFT_725_END_STABILITY=7.9000
PRIMER_RIGHT_725_END_STABILITY=8.2000
PRIMER_PAIR_725_COMPL_ANY=5.00
PRIMER_PAIR_725_COMPL_END=3.00
PRIMER_PAIR_725_PRODUCT_SIZE=189
PRIMER_PAIR_725_PRODUCT_TM=77.6
PRIMER_PAIR_726_PENALTY=8.295537
PRIMER_LEFT_726_PENALTY=6.353121
PRIMER_RIGHT_726_PENALTY=1.942416
PRIMER_LEFT_726_SEQUENCE=TTCTTACACCAGGAGAGGATGGAT
PRIMER_RIGHT_726_SEQUENCE=ATAGTCTGTGTCTGCTCCGC
PRIMER_LEFT_726=602,24
PRIMER_RIGHT_726=850,20
PRIMER_LEFT_726_TM=62.353
PRIMER_RIGHT_726_TM=58.058
PRIMER_LEFT_726_GC_PERCENT=45.833
PRIMER_RIGHT_726_GC_PERCENT=55.000
PRIMER_LEFT_726_SELF_ANY=3.00
PRIMER_RIGHT_726_SELF_ANY=3.00
PRIMER_LEFT_726_SELF_END=2.00
PRIMER_RIGHT_726_SELF_END=2.00
PRIMER_LEFT_726_END_STABILITY=8.1000
PRIMER_RIGHT_726_END_STABILITY=11.4000
PRIMER_PAIR_726_COMPL_ANY=4.00
PRIMER_PAIR_726_COMPL_END=1.00
PRIMER_PAIR_726_PRODUCT_SIZE=249
PRIMER_PAIR_726_PRODUCT_TM=78.7
PRIMER_PAIR_727_PENALTY=8.295879
PRIMER_LEFT_727_PENALTY=3.883954
PRIMER_RIGHT_727_PENALTY=4.411925
PRIMER_LEFT_727_SEQUENCE=ATCAGGGACCAAAACCTGCAT
PRIMER_RIGHT_727_SEQUENCE=AACTCACCAACTAAGCAGCGGT
PRIMER_LEFT_727=367,21
PRIMER_RIGHT_727=564,22
PRIMER_LEFT_727_TM=62.884
PRIMER_RIGHT_727_TM=62.412
PRIMER_LEFT_727_GC_PERCENT=47.619
PRIMER_RIGHT_727_GC_PERCENT=50.000
PRIMER_LEFT_727_SELF_ANY=4.00
PRIMER_RIGHT_727_SELF_ANY=3.00
PRIMER_LEFT_727_SELF_END=2.00
PRIMER_RIGHT_727_SELF_END=2.00
PRIMER_LEFT_727_END_STABILITY=8.4000
PRIMER_RIGHT_727_END_STABILITY=11.1000
PRIMER_PAIR_727_COMPL_ANY=4.00
PRIMER_PAIR_727_COMPL_END=3.00
PRIMER_PAIR_727_PRODUCT_SIZE=198
PRIMER_PAIR_727_PRODUCT_TM=78.2
PRIMER_PAIR_728_PENALTY=8.389490
PRIMER_LEFT_728_PENALTY=5.997786
PRIMER_RIGHT_728_PENALTY=2.391704
PRIMER_LEFT_728_SEQUENCE=GTTGGTGAGTTTGTAAGTGATGCC
PRIMER_RIGHT_728_SEQUENCE=CATCAGCAGAATCCACATTG
PRIMER_LEFT_728=554,24
PRIMER_RIGHT_728=800,20
PRIMER_LEFT_728_TM=61.998
PRIMER_RIGHT_728_TM=57.608
PRIMER_LEFT_728_GC_PERCENT=45.833
PRIMER_RIGHT_728_GC_PERCENT=45.000
PRIMER_LEFT_728_SELF_ANY=2.00
PRIMER_RIGHT_728_SELF_ANY=4.00
PRIMER_LEFT_728_SELF_END=1.00
PRIMER_RIGHT_728_SELF_END=3.00
PRIMER_LEFT_728_END_STABILITY=9.6000
PRIMER_RIGHT_728_END_STABILITY=7.2000
PRIMER_PAIR_728_COMPL_ANY=5.00
PRIMER_PAIR_728_COMPL_END=1.00
PRIMER_PAIR_728_PRODUCT_SIZE=247
PRIMER_PAIR_728_PRODUCT_TM=77.6
PRIMER_PAIR_729_PENALTY=8.495577
PRIMER_LEFT_729_PENALTY=4.008330
PRIMER_RIGHT_729_PENALTY=4.487247
PRIMER_LEFT_729_SEQUENCE=CCAAGAAGTCTACCCTGAACTG
PRIMER_RIGHT_729_SEQUENCE=CTGGTGTAAGAATTTGCACTTG
PRIMER_LEFT_729=418,22
PRIMER_RIGHT_729=613,22
PRIMER_LEFT_729_TM=57.992
PRIMER_RIGHT_729_TM=57.513
PRIMER_LEFT_729_GC_PERCENT=50.000
PRIMER_RIGHT_729_GC_PERCENT=40.909
PRIMER_LEFT_729_SELF_ANY=4.00
PRIMER_RIGHT_729_SELF_ANY=5.00
PRIMER_LEFT_729_SELF_END=1.00
PRIMER_RIGHT_729_SELF_END=3.00
PRIMER_LEFT_729_END_STABILITY=6.7000
PRIMER_RIGHT_729_END_STABILITY=6.7000
PRIMER_PAIR_729_COMPL_ANY=4.00
PRIMER_PAIR_729_COMPL_END=2.00
PRIMER_PAIR_729_PRODUCT_SIZE=196
PRIMER_PAIR_729_PRODUCT_TM=78.0
PRIMER_PAIR_730_PENALTY=8.592124
PRIMER_LEFT_730_PENALTY=4.070165
PRIMER_RIGHT_730_PENALTY=4.521959
PRIMER_LEFT_730_SEQUENCE=AGAATGGGAAGTGGGATTCAGA
PRIMER_RIGHT_730_SEQUENCE=GAGAAGGGCATCACTTACAAACTC
PRIMER_LEFT_730=342,22
PRIMER_RIGHT_730=583,24
PRIMER_LEFT_730_TM=62.070
PRIMER_RIGHT_730_TM=60.522
PRIMER_LEFT_730_GC_PERCENT=45.455
PRIMER_RIGHT_730_GC_PERCENT=45.833
PRIMER_LEFT_730_SELF_ANY=4.00
PRIMER_RIGHT_730_SELF_ANY=4.00
PRIMER_LEFT_730_SELF_END=3.00
PRIMER_RIGHT_730_SELF_END=0.00
PRIMER_LEFT_730_END_STABILITY=6.7000
PRIMER_RIGHT_730_END_STABILITY=6.4000
PRIMER_PAIR_730_COMPL_ANY=5.00
PRIMER_PAIR_730_COMPL_END=2.00
PRIMER_PAIR_730_PRODUCT_SIZE=242
PRIMER_PAIR_730_PRODUCT_TM=78.4
PRIMER_PAIR_731_PENALTY=8.664179
PRIMER_LEFT_731_PENALTY=6.353121
PRIMER_RIGHT_731_PENALTY=2.311058
PRIMER_LEFT_731_SEQUENCE=ATTCTTACACCAGGAGAGGATGGA
PRIMER_RIGHT_731_SEQUENCE=TGGGCAACACACAAACTCTAC
PRIMER_LEFT_731=601,24
PRIMER_RIGHT_731=763,21
PRIMER_LEFT_731_TM=62.353
PRIMER_RIGHT_731_TM=58.689
PRIMER_LEFT_731_GC_PERCENT=45.833
PRIMER_RIGHT_731_GC_PERCENT=47.619
PRIMER_LEFT_731_SELF_ANY=3.00
PRIMER_RIGHT_731_SELF_ANY=2.00
PRIMER_LEFT_731_SELF_END=0.00
PRIMER_RIGHT_731_SELF_END=0.00
PRIMER_LEFT_731_END_STABILITY=8.1000
PRIMER_RIGHT_731_END_STABILITY=5.4000
PRIMER_PAIR_731_COMPL_ANY=4.00
PRIMER_PAIR_731_COMPL_END=2.00
PRIMER_PAIR_731_PRODUCT_SIZE=163
PRIMER_PAIR_731_PRODUCT_TM=76.8
PRIMER_PAIR_732_PENALTY=8.779292
PRIMER_LEFT_732_PENALTY=4.186531
PRIMER_RIGHT_732_PENALTY=4.592761
PRIMER_LEFT_732_SEQUENCE=TGAATGTCCAGAATGGGAAGTG
PRIMER_RIGHT_732_SEQUENCE=AGCGGTAGGGAATCACAAAGTG
PRIMER_LEFT_732=333,22
PRIMER_RIGHT_732=548,22
PRIMER_LEFT_732_TM=62.187
PRIMER_RIGHT_732_TM=62.593
PRIMER_LEFT_732_GC_PERCENT=45.455
PRIMER_RIGHT_732_GC_PERCENT=50.000
PRIMER_LEFT_732_SELF_ANY=3.00
PRIMER_RIGHT_732_SELF_ANY=3.00
PRIMER_LEFT_732_SELF_END=0.00
PRIMER_RIGHT_732_SELF_END=3.00
PRIMER_LEFT_732_END_STABILITY=6.7000
PRIMER_RIGHT_732_END_STABILITY=6.7000
PRIMER_PAIR_732_COMPL_ANY=3.00
PRIMER_PAIR_732_COMPL_END=0.00
PRIMER_PAIR_732_PRODUCT_SIZE=216
PRIMER_PAIR_732_PRODUCT_TM=78.4
PRIMER_PAIR_733_PENALTY=8.793065
PRIMER_LEFT_733_PENALTY=4.232394
PRIMER_RIGHT_733_PENALTY=4.560670
PRIMER_LEFT_733_SEQUENCE=CAGATCCATCAGGGACCA
PRIMER_RIGHT_733_SEQUENCE=GTAAGAATTTGCACTTGTCAGGA
PRIMER_LEFT_733=360,18
PRIMER_RIGHT_733=608,23
PRIMER_LEFT_733_TM=57.768
PRIMER_RIGHT_733_TM=58.439
PRIMER_LEFT_733_GC_PERCENT=55.556
PRIMER_RIGHT_733_GC_PERCENT=39.130
PRIMER_LEFT_733_SELF_ANY=5.00
PRIMER_RIGHT_733_SELF_ANY=4.00
PRIMER_LEFT_733_SELF_END=3.00
PRIMER_RIGHT_733_SELF_END=2.00
PRIMER_LEFT_733_END_STABILITY=7.9000
PRIMER_RIGHT_733_END_STABILITY=8.2000
PRIMER_PAIR_733_COMPL_ANY=3.00
PRIMER_PAIR_733_COMPL_END=1.00
PRIMER_PAIR_733_PRODUCT_SIZE=249
PRIMER_PAIR_733_PRODUCT_TM=78.2
PRIMER_PAIR_734_PENALTY=8.838174
PRIMER_LEFT_734_PENALTY=6.527116
PRIMER_RIGHT_734_PENALTY=2.311058
PRIMER_LEFT_734_SEQUENCE=CAAGTGCAAATTCTTACACCAGGA
PRIMER_RIGHT_734_SEQUENCE=GTGGGCAACACACAAACTCTA
PRIMER_LEFT_734=592,24
PRIMER_RIGHT_734=764,21
PRIMER_LEFT_734_TM=62.527
PRIMER_RIGHT_734_TM=58.689
PRIMER_LEFT_734_GC_PERCENT=41.667
PRIMER_RIGHT_734_GC_PERCENT=47.619
PRIMER_LEFT_734_SELF_ANY=5.00
PRIMER_RIGHT_734_SELF_ANY=3.00
PRIMER_LEFT_734_SELF_END=1.00
PRIMER_RIGHT_734_SELF_END=2.00
PRIMER_LEFT_734_END_STABILITY=8.2000
PRIMER_RIGHT_734_END_STABILITY=5.7000
PRIMER_PAIR_734_COMPL_ANY=3.00
PRIMER_PAIR_734_COMPL_END=0.00
PRIMER_PAIR_734_PRODUCT_SIZE=173
PRIMER_PAIR_734_PRODUCT_TM=77.1
PRIMER_PAIR_735_PENALTY=9.101354
PRIMER_LEFT_735_PENALTY=4.508593
PRIMER_RIGHT_735_PENALTY=4.592761
PRIMER_LEFT_735_SEQUENCE=AGTGGGATTCAGATCCATCAGG
PRIMER_RIGHT_735_SEQUENCE=CAGCGGTAGGGAATCACAAAGT
PRIMER_LEFT_735=351,22
PRIMER_RIGHT_735=549,22
PRIMER_LEFT_735_TM=62.509
PRIMER_RIGHT_735_TM=62.593
PRIMER_LEFT_735_GC_PERCENT=50.000
PRIMER_RIGHT_735_GC_PERCENT=50.000
PRIMER_LEFT_735_SELF_ANY=5.00
PRIMER_RIGHT_735_SELF_ANY=3.00
PRIMER_LEFT_735_SELF_END=2.00
PRIMER_RIGHT_735_SELF_END=1.00
PRIMER_LEFT_735_END_STABILITY=8.2000
PRIMER_RIGHT_735_END_STABILITY=6.7000
PRIMER_PAIR_735_COMPL_ANY=6.00
PRIMER_PAIR_735_COMPL_END=0.00
PRIMER_PAIR_735_PRODUCT_SIZE=199
PRIMER_PAIR_735_PRODUCT_TM=78.5
PRIMER_PAIR_736_PENALTY=9.139055
PRIMER_LEFT_736_PENALTY=4.514227
PRIMER_RIGHT_736_PENALTY=4.624828
PRIMER_LEFT_736_SEQUENCE=CCAAGAAGTCTACCCTGAACTGC
PRIMER_RIGHT_736_SEQUENCE=CTGGTGTAAGAATTTGCACTTGT
PRIMER_LEFT_736=418,23
PRIMER_RIGHT_736=613,23
PRIMER_LEFT_736_TM=61.514
PRIMER_RIGHT_736_TM=58.375
PRIMER_LEFT_736_GC_PERCENT=52.174
PRIMER_RIGHT_736_GC_PERCENT=39.130
PRIMER_LEFT_736_SELF_ANY=4.00
PRIMER_RIGHT_736_SELF_ANY=5.00
PRIMER_LEFT_736_SELF_END=2.00
PRIMER_RIGHT_736_SELF_END=2.00
PRIMER_LEFT_736_END_STABILITY=7.9000
PRIMER_RIGHT_736_END_STABILITY=6.7000
PRIMER_PAIR_736_COMPL_ANY=4.00
PRIMER_PAIR_736_COMPL_END=2.00
PRIMER_PAIR_736_PRODUCT_SIZE=196
PRIMER_PAIR_736_PRODUCT_TM=78.0
PRIMER_PAIR_737_PENALTY=9.146536
PRIMER_LEFT_737_PENALTY=4.514227
PRIMER_RIGHT_737_PENALTY=4.632309
PRIMER_LEFT_737_SEQUENCE=GCCAAGAAGTCTACCCTGAACTG
PRIMER_RIGHT_737_SEQUENCE=AGGGCATCACTTACAAACTCACC
PRIMER_LEFT_737=417,23
PRIMER_RIGHT_737=579,23
PRIMER_LEFT_737_TM=61.514
PRIMER_RIGHT_737_TM=61.632
PRIMER_LEFT_737_GC_PERCENT=52.174
PRIMER_RIGHT_737_GC_PERCENT=47.826
PRIMER_LEFT_737_SELF_ANY=4.00
PRIMER_RIGHT_737_SELF_ANY=2.00
PRIMER_LEFT_737_SELF_END=1.00
PRIMER_RIGHT_737_SELF_END=0.00
PRIMER_LEFT_737_END_STABILITY=6.7000
PRIMER_RIGHT_737_END_STABILITY=7.9000
PRIMER_PAIR_737_COMPL_ANY=5.00
PRIMER_PAIR_737_COMPL_END=1.00
PRIMER_PAIR_737_PRODUCT_SIZE=163
PRIMER_PAIR_737_PRODUCT_TM=78.1
PRIMER_PAIR_738_PENALTY=9.150610
PRIMER_LEFT_738_PENALTY=4.514227
PRIMER_RIGHT_738_PENALTY=4.636384
PRIMER_LEFT_738_SEQUENCE=TGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_738_SEQUENCE=TGGTGTAAGAATTTGCACTTGT
PRIMER_LEFT_738=416,23
PRIMER_RIGHT_738=612,22
PRIMER_LEFT_738_TM=61.514
PRIMER_RIGHT_738_TM=57.364
PRIMER_LEFT_738_GC_PERCENT=47.826
PRIMER_RIGHT_738_GC_PERCENT=36.364
PRIMER_LEFT_738_SELF_ANY=4.00
PRIMER_RIGHT_738_SELF_ANY=5.00
PRIMER_LEFT_738_SELF_END=1.00
PRIMER_RIGHT_738_SELF_END=2.00
PRIMER_LEFT_738_END_STABILITY=6.4000
PRIMER_RIGHT_738_END_STABILITY=6.7000
PRIMER_PAIR_738_COMPL_ANY=4.00
PRIMER_PAIR_738_COMPL_END=1.00
PRIMER_PAIR_738_PRODUCT_SIZE=197
PRIMER_PAIR_738_PRODUCT_TM=77.9
PRIMER_PAIR_739_PENALTY=9.167757
PRIMER_LEFT_739_PENALTY=6.765645
PRIMER_RIGHT_739_PENALTY=2.402111
PRIMER_LEFT_739_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAGT
PRIMER_RIGHT_739_SEQUENCE=CTTCAGCCAGTGGGCAAC
PRIMER_LEFT_739=547,25
PRIMER_RIGHT_739=773,18
PRIMER_LEFT_739_TM=58.234
PRIMER_RIGHT_739_TM=60.402
PRIMER_LEFT_739_GC_PERCENT=40.000
PRIMER_RIGHT_739_GC_PERCENT=61.111
PRIMER_LEFT_739_SELF_ANY=4.00
PRIMER_RIGHT_739_SELF_ANY=5.00
PRIMER_LEFT_739_SELF_END=1.00
PRIMER_RIGHT_739_SELF_END=3.00
PRIMER_LEFT_739_END_STABILITY=5.7000
PRIMER_RIGHT_739_END_STABILITY=8.2000
PRIMER_PAIR_739_COMPL_ANY=4.00
PRIMER_PAIR_739_COMPL_END=2.00
PRIMER_PAIR_739_PRODUCT_SIZE=227
PRIMER_PAIR_739_PRODUCT_TM=77.7
PRIMER_PAIR_740_PENALTY=9.219852
PRIMER_LEFT_740_PENALTY=4.625552
PRIMER_RIGHT_740_PENALTY=4.594300
PRIMER_LEFT_740_SEQUENCE=TGGGAAGTGGGATTCAGA
PRIMER_RIGHT_740_SEQUENCE=CACCAACTAAGCAGCGGT
PRIMER_LEFT_740=346,18
PRIMER_RIGHT_740=560,18
PRIMER_LEFT_740_TM=57.374
PRIMER_RIGHT_740_TM=57.406
PRIMER_LEFT_740_GC_PERCENT=50.000
PRIMER_RIGHT_740_GC_PERCENT=55.556
PRIMER_LEFT_740_SELF_ANY=3.00
PRIMER_RIGHT_740_SELF_ANY=3.00
PRIMER_LEFT_740_SELF_END=3.00
PRIMER_RIGHT_740_SELF_END=2.00
PRIMER_LEFT_740_END_STABILITY=6.7000
PRIMER_RIGHT_740_END_STABILITY=11.1000
PRIMER_PAIR_740_COMPL_ANY=4.00
PRIMER_PAIR_740_COMPL_END=1.00
PRIMER_PAIR_740_PRODUCT_SIZE=215
PRIMER_PAIR_740_PRODUCT_TM=78.7
PRIMER_PAIR_741_PENALTY=9.225005
PRIMER_LEFT_741_PENALTY=6.910331
PRIMER_RIGHT_741_PENALTY=2.314674
PRIMER_LEFT_741_SEQUENCE=GCAAATTCTTACACCAGGAGAGGA
PRIMER_RIGHT_741_SEQUENCE=CATCCTCCTCCGCATCAG
PRIMER_LEFT_741=597,24
PRIMER_RIGHT_741=812,18
PRIMER_LEFT_741_TM=62.910
PRIMER_RIGHT_741_TM=60.315
PRIMER_LEFT_741_GC_PERCENT=45.833
PRIMER_RIGHT_741_GC_PERCENT=61.111
PRIMER_LEFT_741_SELF_ANY=4.00
PRIMER_RIGHT_741_SELF_ANY=2.00
PRIMER_LEFT_741_SELF_END=3.00
PRIMER_RIGHT_741_SELF_END=2.00
PRIMER_LEFT_741_END_STABILITY=7.9000
PRIMER_RIGHT_741_END_STABILITY=6.6000
PRIMER_PAIR_741_COMPL_ANY=6.00
PRIMER_PAIR_741_COMPL_END=3.00
PRIMER_PAIR_741_PRODUCT_SIZE=216
PRIMER_PAIR_741_PRODUCT_TM=77.8
PRIMER_PAIR_742_PENALTY=9.270801
PRIMER_LEFT_742_PENALTY=6.837687
PRIMER_RIGHT_742_PENALTY=2.433114
PRIMER_LEFT_742_SEQUENCE=GTTTGTAAGTGATGCCCTTCTCGT
PRIMER_RIGHT_742_SEQUENCE=GTGGGCAACACACAAACTCTAC
PRIMER_LEFT_742=562,24
PRIMER_RIGHT_742=764,22
PRIMER_LEFT_742_TM=62.838
PRIMER_RIGHT_742_TM=59.567
PRIMER_LEFT_742_GC_PERCENT=45.833
PRIMER_RIGHT_742_GC_PERCENT=50.000
PRIMER_LEFT_742_SELF_ANY=4.00
PRIMER_RIGHT_742_SELF_ANY=3.00
PRIMER_LEFT_742_SELF_END=2.00
PRIMER_RIGHT_742_SELF_END=0.00
PRIMER_LEFT_742_END_STABILITY=8.1000
PRIMER_RIGHT_742_END_STABILITY=5.4000
PRIMER_PAIR_742_COMPL_ANY=7.00
PRIMER_PAIR_742_COMPL_END=2.00
PRIMER_PAIR_742_PRODUCT_SIZE=203
PRIMER_PAIR_742_PRODUCT_TM=77.3
PRIMER_PAIR_743_PENALTY=9.324254
PRIMER_LEFT_743_PENALTY=6.798336
PRIMER_RIGHT_743_PENALTY=2.525918
PRIMER_LEFT_743_SEQUENCE=TTAGTTGGTGAGTTTGTAAGTGATGC
PRIMER_RIGHT_743_SEQUENCE=ATCAGCAGAATCCACATTGTCA
PRIMER_LEFT_743=551,26
PRIMER_RIGHT_743=799,22
PRIMER_LEFT_743_TM=60.798
PRIMER_RIGHT_743_TM=60.526
PRIMER_LEFT_743_GC_PERCENT=38.462
PRIMER_RIGHT_743_GC_PERCENT=40.909
PRIMER_LEFT_743_SELF_ANY=3.00
PRIMER_RIGHT_743_SELF_ANY=5.00
PRIMER_LEFT_743_SELF_END=2.00
PRIMER_RIGHT_743_SELF_END=3.00
PRIMER_LEFT_743_END_STABILITY=8.1000
PRIMER_RIGHT_743_END_STABILITY=6.7000
PRIMER_PAIR_743_COMPL_ANY=4.00
PRIMER_PAIR_743_COMPL_END=2.00
PRIMER_PAIR_743_PRODUCT_SIZE=249
PRIMER_PAIR_743_PRODUCT_TM=77.3
PRIMER_PAIR_744_PENALTY=9.459408
PRIMER_LEFT_744_PENALTY=6.933490
PRIMER_RIGHT_744_PENALTY=2.525918
PRIMER_LEFT_744_SEQUENCE=AGTTTGTAAGTGATGCCCTTCTCG
PRIMER_RIGHT_744_SEQUENCE=CATCAGCAGAATCCACATTGTC
PRIMER_LEFT_744=561,24
PRIMER_RIGHT_744=800,22
PRIMER_LEFT_744_TM=62.933
PRIMER_RIGHT_744_TM=60.526
PRIMER_LEFT_744_GC_PERCENT=45.833
PRIMER_RIGHT_744_GC_PERCENT=45.455
PRIMER_LEFT_744_SELF_ANY=4.00
PRIMER_RIGHT_744_SELF_ANY=5.00
PRIMER_LEFT_744_SELF_END=2.00
PRIMER_RIGHT_744_SELF_END=3.00
PRIMER_LEFT_744_END_STABILITY=8.4000
PRIMER_RIGHT_744_END_STABILITY=6.7000
PRIMER_PAIR_744_COMPL_ANY=5.00
PRIMER_PAIR_744_COMPL_END=1.00
PRIMER_PAIR_744_PRODUCT_SIZE=240
PRIMER_PAIR_744_PRODUCT_TM=77.4
PRIMER_PAIR_745_PENALTY=9.541298
PRIMER_LEFT_745_PENALTY=4.856179
PRIMER_RIGHT_745_PENALTY=4.685119
PRIMER_LEFT_745_SEQUENCE=ATTCAGATCCATCAGGGACCAA
PRIMER_RIGHT_745_SEQUENCE=GGAACGAGAAGGGCATCACTTA
PRIMER_LEFT_745=357,22
PRIMER_RIGHT_745=588,22
PRIMER_LEFT_745_TM=62.856
PRIMER_RIGHT_745_TM=62.685
PRIMER_LEFT_745_GC_PERCENT=45.455
PRIMER_RIGHT_745_GC_PERCENT=50.000
PRIMER_LEFT_745_SELF_ANY=5.00
PRIMER_RIGHT_745_SELF_ANY=4.00
PRIMER_LEFT_745_SELF_END=1.00
PRIMER_RIGHT_745_SELF_END=2.00
PRIMER_LEFT_745_END_STABILITY=8.2000
PRIMER_RIGHT_745_END_STABILITY=5.7000
PRIMER_PAIR_745_COMPL_ANY=3.00
PRIMER_PAIR_745_COMPL_END=1.00
PRIMER_PAIR_745_PRODUCT_SIZE=232
PRIMER_PAIR_745_PRODUCT_TM=78.3
PRIMER_PAIR_746_PENALTY=9.563447
PRIMER_LEFT_746_PENALTY=7.131558
PRIMER_RIGHT_746_PENALTY=2.431889
PRIMER_LEFT_746_SEQUENCE=AGTGCAAATTCTTACACCAGGAGAG
PRIMER_RIGHT_746_SEQUENCE=CCAGTGGGCAACACACAAAC
PRIMER_LEFT_746=594,25
PRIMER_RIGHT_746=767,20
PRIMER_LEFT_746_TM=62.132
PRIMER_RIGHT_746_TM=62.432
PRIMER_LEFT_746_GC_PERCENT=44.000
PRIMER_RIGHT_746_GC_PERCENT=55.000
PRIMER_LEFT_746_SELF_ANY=5.00
PRIMER_RIGHT_746_SELF_ANY=5.00
PRIMER_LEFT_746_SELF_END=0.00
PRIMER_RIGHT_746_SELF_END=0.00
PRIMER_LEFT_746_END_STABILITY=6.4000
PRIMER_RIGHT_746_END_STABILITY=7.0000
PRIMER_PAIR_746_COMPL_ANY=3.00
PRIMER_PAIR_746_COMPL_END=1.00
PRIMER_PAIR_746_PRODUCT_SIZE=174
PRIMER_PAIR_746_PRODUCT_TM=77.2
PRIMER_PAIR_747_PENALTY=9.618247
PRIMER_LEFT_747_PENALTY=7.141896
PRIMER_RIGHT_747_PENALTY=2.476351
PRIMER_LEFT_747_SEQUENCE=TTCCTGACAAGTGCAAATTCTTACA
PRIMER_RIGHT_747_SEQUENCE=CAGTGGGCAACACACAAAC
PRIMER_LEFT_747=585,25
PRIMER_RIGHT_747=766,19
PRIMER_LEFT_747_TM=62.142
PRIMER_RIGHT_747_TM=58.524
PRIMER_LEFT_747_GC_PERCENT=36.000
PRIMER_RIGHT_747_GC_PERCENT=52.632
PRIMER_LEFT_747_SELF_ANY=4.00
PRIMER_RIGHT_747_SELF_ANY=3.00
PRIMER_LEFT_747_SELF_END=3.00
PRIMER_RIGHT_747_SELF_END=0.00
PRIMER_LEFT_747_END_STABILITY=6.0000
PRIMER_RIGHT_747_END_STABILITY=7.0000
PRIMER_PAIR_747_COMPL_ANY=3.00
PRIMER_PAIR_747_COMPL_END=0.00
PRIMER_PAIR_747_PRODUCT_SIZE=182
PRIMER_PAIR_747_PRODUCT_TM=77.1
PRIMER_PAIR_748_PENALTY=9.623396
PRIMER_LEFT_748_PENALTY=7.951017
PRIMER_RIGHT_748_PENALTY=1.672380
PRIMER_LEFT_748_SEQUENCE=GATGTTTGCGAAACTCATCTTCACT
PRIMER_RIGHT_748_SEQUENCE=TGTCTTCACTCCCATCTGCAT
PRIMER_LEFT_748=623,25
PRIMER_RIGHT_748=869,21
PRIMER_LEFT_748_TM=62.951
PRIMER_RIGHT_748_TM=60.672
PRIMER_LEFT_748_GC_PERCENT=40.000
PRIMER_RIGHT_748_GC_PERCENT=47.619
PRIMER_LEFT_748_SELF_ANY=6.00
PRIMER_RIGHT_748_SELF_ANY=4.00
PRIMER_LEFT_748_SELF_END=1.00
PRIMER_RIGHT_748_SELF_END=2.00
PRIMER_LEFT_748_END_STABILITY=6.4000
PRIMER_RIGHT_748_END_STABILITY=8.4000
PRIMER_PAIR_748_COMPL_ANY=4.00
PRIMER_PAIR_748_COMPL_END=0.00
PRIMER_PAIR_748_PRODUCT_SIZE=247
PRIMER_PAIR_748_PRODUCT_TM=78.9
PRIMER_PAIR_749_PENALTY=9.625521
PRIMER_LEFT_749_PENALTY=4.867932
PRIMER_RIGHT_749_PENALTY=4.757589
PRIMER_LEFT_749_SEQUENCE=CAGATCCATCAGGGACCAAAAC
PRIMER_RIGHT_749_SEQUENCE=TGCACTTGTCAGGAACGA
PRIMER_LEFT_749=360,22
PRIMER_RIGHT_749=599,18
PRIMER_LEFT_749_TM=62.868
PRIMER_RIGHT_749_TM=57.242
PRIMER_LEFT_749_GC_PERCENT=50.000
PRIMER_RIGHT_749_GC_PERCENT=50.000
PRIMER_LEFT_749_SELF_ANY=5.00
PRIMER_RIGHT_749_SELF_ANY=4.00
PRIMER_LEFT_749_SELF_END=0.00
PRIMER_RIGHT_749_SELF_END=0.00
PRIMER_LEFT_749_END_STABILITY=7.0000
PRIMER_RIGHT_749_END_STABILITY=8.4000
PRIMER_PAIR_749_COMPL_ANY=3.00
PRIMER_PAIR_749_COMPL_END=0.00
PRIMER_PAIR_749_PRODUCT_SIZE=240
PRIMER_PAIR_749_PRODUCT_TM=78.6
PRIMER_PAIR_750_PENALTY=9.635571
PRIMER_LEFT_750_PENALTY=7.031344
PRIMER_RIGHT_750_PENALTY=2.604227
PRIMER_LEFT_750_SEQUENCE=GTGAGTTTGTAAGTGATGCCCTTCT
PRIMER_RIGHT_750_SEQUENCE=GCATCAGCAGAATCCACATTG
PRIMER_LEFT_750=558,25
PRIMER_RIGHT_750=801,21
PRIMER_LEFT_750_TM=62.031
PRIMER_RIGHT_750_TM=61.604
PRIMER_LEFT_750_GC_PERCENT=44.000
PRIMER_RIGHT_750_GC_PERCENT=47.619
PRIMER_LEFT_750_SELF_ANY=4.00
PRIMER_RIGHT_750_SELF_ANY=4.00
PRIMER_LEFT_750_SELF_END=0.00
PRIMER_RIGHT_750_SELF_END=3.00
PRIMER_LEFT_750_END_STABILITY=6.7000
PRIMER_RIGHT_750_END_STABILITY=7.2000
PRIMER_PAIR_750_COMPL_ANY=6.00
PRIMER_PAIR_750_COMPL_END=1.00
PRIMER_PAIR_750_PRODUCT_SIZE=244
PRIMER_PAIR_750_PRODUCT_TM=77.6
PRIMER_PAIR_751_PENALTY=9.678772
PRIMER_LEFT_751_PENALTY=4.860451
PRIMER_RIGHT_751_PENALTY=4.818321
PRIMER_LEFT_751_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_751_SEQUENCE=TGCACTTGTCAGGAACGAGAAG
PRIMER_LEFT_751=412,24
PRIMER_RIGHT_751=599,22
PRIMER_LEFT_751_TM=60.860
PRIMER_RIGHT_751_TM=62.818
PRIMER_LEFT_751_GC_PERCENT=45.833
PRIMER_RIGHT_751_GC_PERCENT=50.000
PRIMER_LEFT_751_SELF_ANY=5.00
PRIMER_RIGHT_751_SELF_ANY=4.00
PRIMER_LEFT_751_SELF_END=1.00
PRIMER_RIGHT_751_SELF_END=3.00
PRIMER_LEFT_751_END_STABILITY=8.2000
PRIMER_RIGHT_751_END_STABILITY=6.7000
PRIMER_PAIR_751_COMPL_ANY=5.00
PRIMER_PAIR_751_COMPL_END=0.00
PRIMER_PAIR_751_PRODUCT_SIZE=188
PRIMER_PAIR_751_PRODUCT_TM=78.1
PRIMER_PAIR_752_PENALTY=9.709783
PRIMER_LEFT_752_PENALTY=4.867932
PRIMER_RIGHT_752_PENALTY=4.841851
PRIMER_LEFT_752_SEQUENCE=GAATGTCCAGAATGGGAAGTGG
PRIMER_RIGHT_752_SEQUENCE=AAACTCACCAACTAAGCAGCGG
PRIMER_LEFT_752=334,22
PRIMER_RIGHT_752=565,22
PRIMER_LEFT_752_TM=62.868
PRIMER_RIGHT_752_TM=62.842
PRIMER_LEFT_752_GC_PERCENT=50.000
PRIMER_RIGHT_752_GC_PERCENT=50.000
PRIMER_LEFT_752_SELF_ANY=3.00
PRIMER_RIGHT_752_SELF_ANY=3.00
PRIMER_LEFT_752_SELF_END=0.00
PRIMER_RIGHT_752_SELF_END=2.00
PRIMER_LEFT_752_END_STABILITY=7.9000
PRIMER_RIGHT_752_END_STABILITY=11.4000
PRIMER_PAIR_752_COMPL_ANY=4.00
PRIMER_PAIR_752_COMPL_END=0.00
PRIMER_PAIR_752_PRODUCT_SIZE=232
PRIMER_PAIR_752_PRODUCT_TM=78.3
PRIMER_PAIR_753_PENALTY=9.851488
PRIMER_LEFT_753_PENALTY=7.298929
PRIMER_RIGHT_753_PENALTY=2.552559
PRIMER_LEFT_753_SEQUENCE=CAAATTCTTACACCAGGAGAGGATG
PRIMER_RIGHT_753_SEQUENCE=CCCACCAGACATCCGAGT
PRIMER_LEFT_753=598,25
PRIMER_RIGHT_753=830,18
PRIMER_LEFT_753_TM=62.299
PRIMER_RIGHT_753_TM=59.447
PRIMER_LEFT_753_GC_PERCENT=44.000
PRIMER_RIGHT_753_GC_PERCENT=61.111
PRIMER_LEFT_753_SELF_ANY=4.00
PRIMER_RIGHT_753_SELF_ANY=3.00
PRIMER_LEFT_753_SELF_END=0.00
PRIMER_RIGHT_753_SELF_END=3.00
PRIMER_LEFT_753_END_STABILITY=8.1000
PRIMER_RIGHT_753_END_STABILITY=8.1000
PRIMER_PAIR_753_COMPL_ANY=5.00
PRIMER_PAIR_753_COMPL_END=2.00
PRIMER_PAIR_753_PRODUCT_SIZE=233
PRIMER_PAIR_753_PRODUCT_TM=78.3
PRIMER_PAIR_754_PENALTY=9.912956
PRIMER_LEFT_754_PENALTY=4.973389
PRIMER_RIGHT_754_PENALTY=4.939568
PRIMER_LEFT_754_SEQUENCE=CAGAATGGGAAGTGGGATTCAG
PRIMER_RIGHT_754_SEQUENCE=AGAAGGGCATCACTTACAAACT
PRIMER_LEFT_754=341,22
PRIMER_RIGHT_754=582,22
PRIMER_LEFT_754_TM=62.973
PRIMER_RIGHT_754_TM=57.060
PRIMER_LEFT_754_GC_PERCENT=50.000
PRIMER_RIGHT_754_GC_PERCENT=40.909
PRIMER_LEFT_754_SELF_ANY=4.00
PRIMER_RIGHT_754_SELF_ANY=4.00
PRIMER_LEFT_754_SELF_END=2.00
PRIMER_RIGHT_754_SELF_END=1.00
PRIMER_LEFT_754_END_STABILITY=7.0000
PRIMER_RIGHT_754_END_STABILITY=6.7000
PRIMER_PAIR_754_COMPL_ANY=5.00
PRIMER_PAIR_754_COMPL_END=2.00
PRIMER_PAIR_754_PRODUCT_SIZE=242
PRIMER_PAIR_754_PRODUCT_TM=78.4
PRIMER_PAIR_755_PENALTY=10.000121
PRIMER_LEFT_755_PENALTY=7.384820
PRIMER_RIGHT_755_PENALTY=2.615302
PRIMER_LEFT_755_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGTGA
PRIMER_RIGHT_755_SEQUENCE=CCAGTGGGCAACACACAAA
PRIMER_LEFT_755=548,26
PRIMER_RIGHT_755=767,19
PRIMER_LEFT_755_TM=61.385
PRIMER_RIGHT_755_TM=61.615
PRIMER_LEFT_755_GC_PERCENT=38.462
PRIMER_RIGHT_755_GC_PERCENT=52.632
PRIMER_LEFT_755_SELF_ANY=4.00
PRIMER_RIGHT_755_SELF_ANY=5.00
PRIMER_LEFT_755_SELF_END=1.00
PRIMER_RIGHT_755_SELF_END=0.00
PRIMER_LEFT_755_END_STABILITY=6.4000
PRIMER_RIGHT_755_END_STABILITY=7.0000
PRIMER_PAIR_755_COMPL_ANY=6.00
PRIMER_PAIR_755_COMPL_END=3.00
PRIMER_PAIR_755_PRODUCT_SIZE=220
PRIMER_PAIR_755_PRODUCT_TM=77.5
PRIMER_PAIR_756_PENALTY=10.032035
PRIMER_LEFT_756_PENALTY=7.438416
PRIMER_RIGHT_756_PENALTY=2.593620
PRIMER_LEFT_756_SEQUENCE=AAGTGCAAATTCTTACACCAGGAGA
PRIMER_RIGHT_756_SEQUENCE=CAGTGGGCAACACACAAACTC
PRIMER_LEFT_756=593,25
PRIMER_RIGHT_756=766,21
PRIMER_LEFT_756_TM=62.438
PRIMER_RIGHT_756_TM=61.594
PRIMER_LEFT_756_GC_PERCENT=40.000
PRIMER_RIGHT_756_GC_PERCENT=52.381
PRIMER_LEFT_756_SELF_ANY=5.00
PRIMER_RIGHT_756_SELF_ANY=3.00
PRIMER_LEFT_756_SELF_END=1.00
PRIMER_RIGHT_756_SELF_END=0.00
PRIMER_LEFT_756_END_STABILITY=7.9000
PRIMER_RIGHT_756_END_STABILITY=6.4000
PRIMER_PAIR_756_COMPL_ANY=3.00
PRIMER_PAIR_756_COMPL_END=2.00
PRIMER_PAIR_756_PRODUCT_SIZE=174
PRIMER_PAIR_756_PRODUCT_TM=77.0
PRIMER_PAIR_757_PENALTY=10.067159
PRIMER_LEFT_757_PENALTY=7.480183
PRIMER_RIGHT_757_PENALTY=2.586975
PRIMER_LEFT_757_SEQUENCE=ATTCTTACACCAGGAGAGGATGGAT
PRIMER_RIGHT_757_SEQUENCE=GTCTGTGTCTGCTCCGCC
PRIMER_LEFT_757=601,25
PRIMER_RIGHT_757=847,18
PRIMER_LEFT_757_TM=62.480
PRIMER_RIGHT_757_TM=60.587
PRIMER_LEFT_757_GC_PERCENT=44.000
PRIMER_RIGHT_757_GC_PERCENT=66.667
PRIMER_LEFT_757_SELF_ANY=3.00
PRIMER_RIGHT_757_SELF_ANY=2.00
PRIMER_LEFT_757_SELF_END=2.00
PRIMER_RIGHT_757_SELF_END=1.00
PRIMER_LEFT_757_END_STABILITY=8.1000
PRIMER_RIGHT_757_END_STABILITY=12.9000
PRIMER_PAIR_757_COMPL_ANY=4.00
PRIMER_PAIR_757_COMPL_END=1.00
PRIMER_PAIR_757_PRODUCT_SIZE=247
PRIMER_PAIR_757_PRODUCT_TM=78.9
PRIMER_PAIR_758_PENALTY=10.117496
PRIMER_LEFT_758_PENALTY=7.358003
PRIMER_RIGHT_758_PENALTY=2.759493
PRIMER_LEFT_758_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTAAGT
PRIMER_RIGHT_758_SEQUENCE=AGCCAGTGGGCAACACAC
PRIMER_LEFT_758=546,26
PRIMER_RIGHT_758=769,18
PRIMER_LEFT_758_TM=61.358
PRIMER_RIGHT_758_TM=60.759
PRIMER_LEFT_758_GC_PERCENT=42.308
PRIMER_RIGHT_758_GC_PERCENT=61.111
PRIMER_LEFT_758_SELF_ANY=4.00
PRIMER_RIGHT_758_SELF_ANY=5.00
PRIMER_LEFT_758_SELF_END=1.00
PRIMER_RIGHT_758_SELF_END=3.00
PRIMER_LEFT_758_END_STABILITY=5.7000
PRIMER_RIGHT_758_END_STABILITY=6.4000
PRIMER_PAIR_758_COMPL_ANY=5.00
PRIMER_PAIR_758_COMPL_END=2.00
PRIMER_PAIR_758_PRODUCT_SIZE=224
PRIMER_PAIR_758_PRODUCT_TM=77.7
PRIMER_PAIR_759_PENALTY=10.128958
PRIMER_LEFT_759_PENALTY=5.291723
PRIMER_RIGHT_759_PENALTY=4.837235
PRIMER_LEFT_759_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_759_SEQUENCE=TGTCAGGAACGAGAAGGG
PRIMER_LEFT_759=413,24
PRIMER_RIGHT_759=593,18
PRIMER_LEFT_759_TM=61.292
PRIMER_RIGHT_759_TM=57.163
PRIMER_LEFT_759_GC_PERCENT=41.667
PRIMER_RIGHT_759_GC_PERCENT=55.556
PRIMER_LEFT_759_SELF_ANY=5.00
PRIMER_RIGHT_759_SELF_ANY=3.00
PRIMER_LEFT_759_SELF_END=0.00
PRIMER_RIGHT_759_SELF_END=0.00
PRIMER_LEFT_759_END_STABILITY=7.0000
PRIMER_RIGHT_759_END_STABILITY=9.7000
PRIMER_PAIR_759_COMPL_ANY=5.00
PRIMER_PAIR_759_COMPL_END=1.00
PRIMER_PAIR_759_PRODUCT_SIZE=181
PRIMER_PAIR_759_PRODUCT_TM=77.9
PRIMER_PAIR_760_PENALTY=10.202636
PRIMER_LEFT_760_PENALTY=5.219386
PRIMER_RIGHT_760_PENALTY=4.983250
PRIMER_LEFT_760_SEQUENCE=AGAATGGGAAGTGGGATTCAGAT
PRIMER_RIGHT_760_SEQUENCE=GCGGTAGGGAATCACAAA
PRIMER_LEFT_760=342,23
PRIMER_RIGHT_760=547,18
PRIMER_LEFT_760_TM=62.219
PRIMER_RIGHT_760_TM=57.017
PRIMER_LEFT_760_GC_PERCENT=43.478
PRIMER_RIGHT_760_GC_PERCENT=50.000
PRIMER_LEFT_760_SELF_ANY=4.00
PRIMER_RIGHT_760_SELF_ANY=3.00
PRIMER_LEFT_760_SELF_END=2.00
PRIMER_RIGHT_760_SELF_END=1.00
PRIMER_LEFT_760_END_STABILITY=6.6000
PRIMER_RIGHT_760_END_STABILITY=7.0000
PRIMER_PAIR_760_COMPL_ANY=6.00
PRIMER_PAIR_760_COMPL_END=2.00
PRIMER_PAIR_760_PRODUCT_SIZE=206
PRIMER_PAIR_760_PRODUCT_TM=78.5
PRIMER_PAIR_761_PENALTY=10.320870
PRIMER_LEFT_761_PENALTY=5.335483
PRIMER_RIGHT_761_PENALTY=4.985387
PRIMER_LEFT_761_SEQUENCE=ATGAATGTCCAGAATGGGAAGTG
PRIMER_RIGHT_761_SEQUENCE=TGGGGATGGGTCTTGCAC
PRIMER_LEFT_761=332,23
PRIMER_RIGHT_761=528,18
PRIMER_LEFT_761_TM=62.335
PRIMER_RIGHT_761_TM=62.985
PRIMER_LEFT_761_GC_PERCENT=43.478
PRIMER_RIGHT_761_GC_PERCENT=61.111
PRIMER_LEFT_761_SELF_ANY=3.00
PRIMER_RIGHT_761_SELF_ANY=4.00
PRIMER_LEFT_761_SELF_END=0.00
PRIMER_RIGHT_761_SELF_END=2.00
PRIMER_LEFT_761_END_STABILITY=6.7000
PRIMER_RIGHT_761_END_STABILITY=8.2000
PRIMER_PAIR_761_COMPL_ANY=3.00
PRIMER_PAIR_761_COMPL_END=3.00
PRIMER_PAIR_761_PRODUCT_SIZE=197
PRIMER_PAIR_761_PRODUCT_TM=78.1
PRIMER_PAIR_762_PENALTY=10.327516
PRIMER_LEFT_762_PENALTY=7.470065
PRIMER_RIGHT_762_PENALTY=2.857451
PRIMER_LEFT_762_SEQUENCE=TTAGTTGGTGAGTTTGTAAGTGATG
PRIMER_RIGHT_762_SEQUENCE=AAACTCTACCCCTCGGAACTTG
PRIMER_LEFT_762=551,25
PRIMER_RIGHT_762=751,22
PRIMER_LEFT_762_TM=57.530
PRIMER_RIGHT_762_TM=60.857
PRIMER_LEFT_762_GC_PERCENT=36.000
PRIMER_RIGHT_762_GC_PERCENT=50.000
PRIMER_LEFT_762_SELF_ANY=3.00
PRIMER_RIGHT_762_SELF_ANY=3.00
PRIMER_LEFT_762_SELF_END=0.00
PRIMER_RIGHT_762_SELF_END=1.00
PRIMER_LEFT_762_END_STABILITY=6.9000
PRIMER_RIGHT_762_END_STABILITY=6.7000
PRIMER_PAIR_762_COMPL_ANY=7.00
PRIMER_PAIR_762_COMPL_END=1.00
PRIMER_PAIR_762_PRODUCT_SIZE=201
PRIMER_PAIR_762_PRODUCT_TM=76.7
PRIMER_PAIR_763_PENALTY=10.334806
PRIMER_LEFT_763_PENALTY=7.554905
PRIMER_RIGHT_763_PENALTY=2.779901
PRIMER_LEFT_763_SEQUENCE=TGACAAGTGCAAATTCTTACACCAG
PRIMER_RIGHT_763_SEQUENCE=CTCCTCCGCATCAGCAGAAT
PRIMER_LEFT_763=589,25
PRIMER_RIGHT_763=808,20
PRIMER_LEFT_763_TM=62.555
PRIMER_RIGHT_763_TM=62.780
PRIMER_LEFT_763_GC_PERCENT=40.000
PRIMER_RIGHT_763_GC_PERCENT=55.000
PRIMER_LEFT_763_SELF_ANY=5.00
PRIMER_RIGHT_763_SELF_ANY=4.00
PRIMER_LEFT_763_SELF_END=3.00
PRIMER_RIGHT_763_SELF_END=2.00
PRIMER_LEFT_763_END_STABILITY=7.9000
PRIMER_RIGHT_763_END_STABILITY=6.6000
PRIMER_PAIR_763_COMPL_ANY=5.00
PRIMER_PAIR_763_COMPL_END=0.00
PRIMER_PAIR_763_PRODUCT_SIZE=220
PRIMER_PAIR_763_PRODUCT_TM=77.7
PRIMER_PAIR_764_PENALTY=10.352207
PRIMER_LEFT_764_PENALTY=5.335483
PRIMER_RIGHT_764_PENALTY=5.016724
PRIMER_LEFT_764_SEQUENCE=CATGAATGTCCAGAATGGGAAGT
PRIMER_RIGHT_764_SEQUENCE=ACTCACCAACTAAGCAGCGGTAG
PRIMER_LEFT_764=331,23
PRIMER_RIGHT_764=563,23
PRIMER_LEFT_764_TM=62.335
PRIMER_RIGHT_764_TM=62.017
PRIMER_LEFT_764_GC_PERCENT=43.478
PRIMER_RIGHT_764_GC_PERCENT=52.174
PRIMER_LEFT_764_SELF_ANY=4.00
PRIMER_RIGHT_764_SELF_ANY=3.00
PRIMER_LEFT_764_SELF_END=1.00
PRIMER_RIGHT_764_SELF_END=3.00
PRIMER_LEFT_764_END_STABILITY=6.4000
PRIMER_RIGHT_764_END_STABILITY=6.9000
PRIMER_PAIR_764_COMPL_ANY=4.00
PRIMER_PAIR_764_COMPL_END=0.00
PRIMER_PAIR_764_PRODUCT_SIZE=233
PRIMER_PAIR_764_PRODUCT_TM=78.4
PRIMER_PAIR_765_PENALTY=10.405221
PRIMER_LEFT_765_PENALTY=7.547770
PRIMER_RIGHT_765_PENALTY=2.857451
PRIMER_LEFT_765_SEQUENCE=CTTAGTTGGTGAGTTTGTAAGTGATG
PRIMER_RIGHT_765_SEQUENCE=CAAACTCTACCCCTCGGAACTT
PRIMER_LEFT_765=550,26
PRIMER_RIGHT_765=752,22
PRIMER_LEFT_765_TM=58.452
PRIMER_RIGHT_765_TM=60.857
PRIMER_LEFT_765_GC_PERCENT=38.462
PRIMER_RIGHT_765_GC_PERCENT=50.000
PRIMER_LEFT_765_SELF_ANY=4.00
PRIMER_RIGHT_765_SELF_ANY=3.00
PRIMER_LEFT_765_SELF_END=0.00
PRIMER_RIGHT_765_SELF_END=3.00
PRIMER_LEFT_765_END_STABILITY=6.9000
PRIMER_RIGHT_765_END_STABILITY=6.7000
PRIMER_PAIR_765_COMPL_ANY=8.00
PRIMER_PAIR_765_COMPL_END=2.00
PRIMER_PAIR_765_PRODUCT_SIZE=203
PRIMER_PAIR_765_PRODUCT_TM=76.9
PRIMER_PAIR_766_PENALTY=10.438841
PRIMER_LEFT_766_PENALTY=5.409997
PRIMER_RIGHT_766_PENALTY=5.028844
PRIMER_LEFT_766_SEQUENCE=AGTATTGCCAAGAAGTCTACCCT
PRIMER_RIGHT_766_SEQUENCE=TGTAAGAATTTGCACTTGTCAGGA
PRIMER_LEFT_766=411,23
PRIMER_RIGHT_766=609,24
PRIMER_LEFT_766_TM=57.590
PRIMER_RIGHT_766_TM=61.029
PRIMER_LEFT_766_GC_PERCENT=43.478
PRIMER_RIGHT_766_GC_PERCENT=37.500
PRIMER_LEFT_766_SELF_ANY=5.00
PRIMER_RIGHT_766_SELF_ANY=4.00
PRIMER_LEFT_766_SELF_END=2.00
PRIMER_RIGHT_766_SELF_END=2.00
PRIMER_LEFT_766_END_STABILITY=9.1000
PRIMER_RIGHT_766_END_STABILITY=8.2000
PRIMER_PAIR_766_COMPL_ANY=5.00
PRIMER_PAIR_766_COMPL_END=2.00
PRIMER_PAIR_766_PRODUCT_SIZE=199
PRIMER_PAIR_766_PRODUCT_TM=77.5
PRIMER_PAIR_767_PENALTY=10.444589
PRIMER_LEFT_767_PENALTY=7.554905
PRIMER_RIGHT_767_PENALTY=2.889684
PRIMER_LEFT_767_SEQUENCE=CTGACAAGTGCAAATTCTTACACCA
PRIMER_RIGHT_767_SEQUENCE=CTCCGCATCAGCAGAATCC
PRIMER_LEFT_767=588,25
PRIMER_RIGHT_767=805,19
PRIMER_LEFT_767_TM=62.555
PRIMER_RIGHT_767_TM=61.890
PRIMER_LEFT_767_GC_PERCENT=40.000
PRIMER_RIGHT_767_GC_PERCENT=57.895
PRIMER_LEFT_767_SELF_ANY=5.00
PRIMER_RIGHT_767_SELF_ANY=4.00
PRIMER_LEFT_767_SELF_END=1.00
PRIMER_RIGHT_767_SELF_END=2.00
PRIMER_LEFT_767_END_STABILITY=8.2000
PRIMER_RIGHT_767_END_STABILITY=8.1000
PRIMER_PAIR_767_COMPL_ANY=5.00
PRIMER_PAIR_767_COMPL_END=0.00
PRIMER_PAIR_767_PRODUCT_SIZE=218
PRIMER_PAIR_767_PRODUCT_TM=77.7
PRIMER_PAIR_768_PENALTY=10.507809
PRIMER_LEFT_768_PENALTY=7.886984
PRIMER_RIGHT_768_PENALTY=2.620825
PRIMER_LEFT_768_SEQUENCE=AAATTCTTACACCAGGAGAGGATGG
PRIMER_RIGHT_768_SEQUENCE=AGTCTGTGTCTGCTCCGCC
PRIMER_LEFT_768=599,25
PRIMER_RIGHT_768=848,19
PRIMER_LEFT_768_TM=62.887
PRIMER_RIGHT_768_TM=61.621
PRIMER_LEFT_768_GC_PERCENT=44.000
PRIMER_RIGHT_768_GC_PERCENT=63.158
PRIMER_LEFT_768_SELF_ANY=4.00
PRIMER_RIGHT_768_SELF_ANY=3.00
PRIMER_LEFT_768_SELF_END=0.00
PRIMER_RIGHT_768_SELF_END=1.00
PRIMER_LEFT_768_END_STABILITY=8.1000
PRIMER_RIGHT_768_END_STABILITY=12.9000
PRIMER_PAIR_768_COMPL_ANY=4.00
PRIMER_PAIR_768_COMPL_END=2.00
PRIMER_PAIR_768_PRODUCT_SIZE=250
PRIMER_PAIR_768_PRODUCT_TM=78.7
PRIMER_PAIR_769_PENALTY=10.529548
PRIMER_LEFT_769_PENALTY=5.335483
PRIMER_RIGHT_769_PENALTY=5.194065
PRIMER_LEFT_769_SEQUENCE=ACATGAATGTCCAGAATGGGAAG
PRIMER_RIGHT_769_SEQUENCE=CACTTACAAACTCACCAACTAAGCA
PRIMER_LEFT_769=330,23
PRIMER_RIGHT_769=572,25
PRIMER_LEFT_769_TM=62.335
PRIMER_RIGHT_769_TM=59.806
PRIMER_LEFT_769_GC_PERCENT=43.478
PRIMER_RIGHT_769_GC_PERCENT=40.000
PRIMER_LEFT_769_SELF_ANY=6.00
PRIMER_RIGHT_769_SELF_ANY=4.00
PRIMER_LEFT_769_SELF_END=0.00
PRIMER_RIGHT_769_SELF_END=0.00
PRIMER_LEFT_769_END_STABILITY=8.2000
PRIMER_RIGHT_769_END_STABILITY=8.5000
PRIMER_PAIR_769_COMPL_ANY=3.00
PRIMER_PAIR_769_COMPL_END=1.00
PRIMER_PAIR_769_PRODUCT_SIZE=243
PRIMER_PAIR_769_PRODUCT_TM=78.2
PRIMER_PAIR_770_PENALTY=10.618447
PRIMER_LEFT_770_PENALTY=7.751085
PRIMER_RIGHT_770_PENALTY=2.867362
PRIMER_LEFT_770_SEQUENCE=AGTTGGTGAGTTTGTAAGTGATGCC
PRIMER_RIGHT_770_SEQUENCE=TCAGCAGAATCCACATTGTC
PRIMER_LEFT_770=553,25
PRIMER_RIGHT_770=798,20
PRIMER_LEFT_770_TM=62.751
PRIMER_RIGHT_770_TM=57.133
PRIMER_LEFT_770_GC_PERCENT=44.000
PRIMER_RIGHT_770_GC_PERCENT=45.000
PRIMER_LEFT_770_SELF_ANY=2.00
PRIMER_RIGHT_770_SELF_ANY=5.00
PRIMER_LEFT_770_SELF_END=1.00
PRIMER_RIGHT_770_SELF_END=3.00
PRIMER_LEFT_770_END_STABILITY=9.6000
PRIMER_RIGHT_770_END_STABILITY=6.7000
PRIMER_PAIR_770_COMPL_ANY=4.00
PRIMER_PAIR_770_COMPL_END=1.00
PRIMER_PAIR_770_PRODUCT_SIZE=246
PRIMER_PAIR_770_PRODUCT_TM=77.5
PRIMER_PAIR_771_PENALTY=10.824667
PRIMER_LEFT_771_PENALTY=7.934983
PRIMER_RIGHT_771_PENALTY=2.889684
PRIMER_LEFT_771_SEQUENCE=TGAGTTTGTAAGTGATGCCCTTCTC
PRIMER_RIGHT_771_SEQUENCE=CCTCCGCATCAGCAGAATC
PRIMER_LEFT_771=559,25
PRIMER_RIGHT_771=806,19
PRIMER_LEFT_771_TM=62.935
PRIMER_RIGHT_771_TM=61.890
PRIMER_LEFT_771_GC_PERCENT=44.000
PRIMER_RIGHT_771_GC_PERCENT=57.895
PRIMER_LEFT_771_SELF_ANY=4.00
PRIMER_RIGHT_771_SELF_ANY=4.00
PRIMER_LEFT_771_SELF_END=2.00
PRIMER_RIGHT_771_SELF_END=3.00
PRIMER_LEFT_771_END_STABILITY=6.7000
PRIMER_RIGHT_771_END_STABILITY=6.6000
PRIMER_PAIR_771_COMPL_ANY=6.00
PRIMER_PAIR_771_COMPL_END=1.00
PRIMER_PAIR_771_PRODUCT_SIZE=248
PRIMER_PAIR_771_PRODUCT_TM=77.8
PRIMER_PAIR_772_PENALTY=11.012563
PRIMER_LEFT_772_PENALTY=5.821907
PRIMER_RIGHT_772_PENALTY=5.190656
PRIMER_LEFT_772_SEQUENCE=TGCAGTATTGCCAAGAAGTCTACC
PRIMER_RIGHT_772_SEQUENCE=CGAGAAGGGCATCACTTACAAAC
PRIMER_LEFT_772=408,24
PRIMER_RIGHT_772=584,23
PRIMER_LEFT_772_TM=61.822
PRIMER_RIGHT_772_TM=62.191
PRIMER_LEFT_772_GC_PERCENT=45.833
PRIMER_RIGHT_772_GC_PERCENT=47.826
PRIMER_LEFT_772_SELF_ANY=6.00
PRIMER_RIGHT_772_SELF_ANY=4.00
PRIMER_LEFT_772_SELF_END=1.00
PRIMER_RIGHT_772_SELF_END=0.00
PRIMER_LEFT_772_END_STABILITY=6.9000
PRIMER_RIGHT_772_END_STABILITY=7.0000
PRIMER_PAIR_772_COMPL_ANY=5.00
PRIMER_PAIR_772_COMPL_END=0.00
PRIMER_PAIR_772_PRODUCT_SIZE=177
PRIMER_PAIR_772_PRODUCT_TM=78.1
PRIMER_PAIR_773_PENALTY=11.191086
PRIMER_LEFT_773_PENALTY=6.000430
PRIMER_RIGHT_773_PENALTY=5.190656
PRIMER_LEFT_773_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_773_SEQUENCE=ACGAGAAGGGCATCACTTACAAA
PRIMER_LEFT_773=414,24
PRIMER_RIGHT_773=585,23
PRIMER_LEFT_773_TM=62.000
PRIMER_RIGHT_773_TM=62.191
PRIMER_LEFT_773_GC_PERCENT=45.833
PRIMER_RIGHT_773_GC_PERCENT=43.478
PRIMER_LEFT_773_SELF_ANY=5.00
PRIMER_RIGHT_773_SELF_ANY=4.00
PRIMER_LEFT_773_SELF_END=0.00
PRIMER_RIGHT_773_SELF_END=1.00
PRIMER_LEFT_773_END_STABILITY=6.7000
PRIMER_RIGHT_773_END_STABILITY=7.0000
PRIMER_PAIR_773_COMPL_ANY=5.00
PRIMER_PAIR_773_COMPL_END=0.00
PRIMER_PAIR_773_PRODUCT_SIZE=172
PRIMER_PAIR_773_PRODUCT_TM=77.9
PRIMER_PAIR_774_PENALTY=11.441555
PRIMER_LEFT_774_PENALTY=8.548866
PRIMER_RIGHT_774_PENALTY=2.892689
PRIMER_LEFT_774_SEQUENCE=CTTAGTTGGTGAGTTTGTAAGTGATGC
PRIMER_RIGHT_774_SEQUENCE=ACTCTACCCCTCGGAACTTGTC
PRIMER_LEFT_774=550,27
PRIMER_RIGHT_774=749,22
PRIMER_LEFT_774_TM=61.549
PRIMER_RIGHT_774_TM=60.893
PRIMER_LEFT_774_GC_PERCENT=40.741
PRIMER_RIGHT_774_GC_PERCENT=54.545
PRIMER_LEFT_774_SELF_ANY=4.00
PRIMER_RIGHT_774_SELF_ANY=3.00
PRIMER_LEFT_774_SELF_END=2.00
PRIMER_RIGHT_774_SELF_END=1.00
PRIMER_LEFT_774_END_STABILITY=8.1000
PRIMER_RIGHT_774_END_STABILITY=6.7000
PRIMER_PAIR_774_COMPL_ANY=5.00
PRIMER_PAIR_774_COMPL_END=0.00
PRIMER_PAIR_774_PRODUCT_SIZE=200
PRIMER_PAIR_774_PRODUCT_TM=77.0
PRIMER_PAIR_775_PENALTY=11.444445
PRIMER_LEFT_775_PENALTY=8.548866
PRIMER_RIGHT_775_PENALTY=2.895578
PRIMER_LEFT_775_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTGATG
PRIMER_RIGHT_775_SEQUENCE=GCAGAATCCACATTGTCACTTT
PRIMER_LEFT_775=549,27
PRIMER_RIGHT_775=795,22
PRIMER_LEFT_775_TM=61.549
PRIMER_RIGHT_775_TM=59.104
PRIMER_LEFT_775_GC_PERCENT=40.741
PRIMER_RIGHT_775_GC_PERCENT=40.909
PRIMER_LEFT_775_SELF_ANY=4.00
PRIMER_RIGHT_775_SELF_ANY=5.00
PRIMER_LEFT_775_SELF_END=0.00
PRIMER_RIGHT_775_SELF_END=3.00
PRIMER_LEFT_775_END_STABILITY=6.9000
PRIMER_RIGHT_775_END_STABILITY=6.7000
PRIMER_PAIR_775_COMPL_ANY=6.00
PRIMER_PAIR_775_COMPL_END=3.00
PRIMER_PAIR_775_PRODUCT_SIZE=247
PRIMER_PAIR_775_PRODUCT_TM=77.6
PRIMER_PAIR_776_PENALTY=11.491456
PRIMER_LEFT_776_PENALTY=8.548866
PRIMER_RIGHT_776_PENALTY=2.942589
PRIMER_LEFT_776_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGTGAT
PRIMER_RIGHT_776_SEQUENCE=TCTACCCCTCGGAACTTGTCA
PRIMER_LEFT_776=548,27
PRIMER_RIGHT_776=747,21
PRIMER_LEFT_776_TM=61.549
PRIMER_RIGHT_776_TM=61.943
PRIMER_LEFT_776_GC_PERCENT=37.037
PRIMER_RIGHT_776_GC_PERCENT=52.381
PRIMER_LEFT_776_SELF_ANY=4.00
PRIMER_RIGHT_776_SELF_ANY=3.00
PRIMER_LEFT_776_SELF_END=2.00
PRIMER_RIGHT_776_SELF_END=3.00
PRIMER_LEFT_776_END_STABILITY=6.3000
PRIMER_RIGHT_776_END_STABILITY=6.7000
PRIMER_PAIR_776_COMPL_ANY=4.00
PRIMER_PAIR_776_COMPL_END=2.00
PRIMER_PAIR_776_PRODUCT_SIZE=200
PRIMER_PAIR_776_PRODUCT_TM=77.0
PRIMER_PAIR_777_PENALTY=11.629422
PRIMER_LEFT_777_PENALTY=6.438766
PRIMER_RIGHT_777_PENALTY=5.190656
PRIMER_LEFT_777_SEQUENCE=GCAGTATTGCCAAGAAGTCTACCC
PRIMER_RIGHT_777_SEQUENCE=AACGAGAAGGGCATCACTTACAA
PRIMER_LEFT_777=409,24
PRIMER_RIGHT_777=586,23
PRIMER_LEFT_777_TM=62.439
PRIMER_RIGHT_777_TM=62.191
PRIMER_LEFT_777_GC_PERCENT=50.000
PRIMER_RIGHT_777_GC_PERCENT=43.478
PRIMER_LEFT_777_SELF_ANY=6.00
PRIMER_RIGHT_777_SELF_ANY=4.00
PRIMER_LEFT_777_SELF_END=0.00
PRIMER_RIGHT_777_SELF_END=2.00
PRIMER_LEFT_777_END_STABILITY=8.4000
PRIMER_RIGHT_777_END_STABILITY=6.0000
PRIMER_PAIR_777_COMPL_ANY=5.00
PRIMER_PAIR_777_COMPL_END=1.00
PRIMER_PAIR_777_PRODUCT_SIZE=178
PRIMER_PAIR_777_PRODUCT_TM=78.0
PRIMER_PAIR_778_PENALTY=11.652859
PRIMER_LEFT_778_PENALTY=8.671003
PRIMER_RIGHT_778_PENALTY=2.981856
PRIMER_LEFT_778_SEQUENCE=TAGTTGGTGAGTTTGTAAGTGATGCC
PRIMER_RIGHT_778_SEQUENCE=ACCCCTCGGAACTTGTCA
PRIMER_LEFT_778=552,26
PRIMER_RIGHT_778=744,18
PRIMER_LEFT_778_TM=62.671
PRIMER_RIGHT_778_TM=59.018
PRIMER_LEFT_778_GC_PERCENT=42.308
PRIMER_RIGHT_778_GC_PERCENT=55.556
PRIMER_LEFT_778_SELF_ANY=2.00
PRIMER_RIGHT_778_SELF_ANY=3.00
PRIMER_LEFT_778_SELF_END=1.00
PRIMER_RIGHT_778_SELF_END=3.00
PRIMER_LEFT_778_END_STABILITY=9.6000
PRIMER_RIGHT_778_END_STABILITY=6.7000
PRIMER_PAIR_778_COMPL_ANY=4.00
PRIMER_PAIR_778_COMPL_END=2.00
PRIMER_PAIR_778_PRODUCT_SIZE=193
PRIMER_PAIR_778_PRODUCT_TM=77.0
PRIMER_PAIR_779_PENALTY=11.656166
PRIMER_LEFT_779_PENALTY=8.766482
PRIMER_RIGHT_779_PENALTY=2.889684
PRIMER_LEFT_779_SEQUENCE=GTTCCTGACAAGTGCAAATTCTTACA
PRIMER_RIGHT_779_SEQUENCE=TCCTCCGCATCAGCAGAAT
PRIMER_LEFT_779=584,26
PRIMER_RIGHT_779=807,19
PRIMER_LEFT_779_TM=62.766
PRIMER_RIGHT_779_TM=61.890
PRIMER_LEFT_779_GC_PERCENT=38.462
PRIMER_RIGHT_779_GC_PERCENT=52.632
PRIMER_LEFT_779_SELF_ANY=4.00
PRIMER_RIGHT_779_SELF_ANY=4.00
PRIMER_LEFT_779_SELF_END=3.00
PRIMER_RIGHT_779_SELF_END=2.00
PRIMER_LEFT_779_END_STABILITY=6.0000
PRIMER_RIGHT_779_END_STABILITY=6.6000
PRIMER_PAIR_779_COMPL_ANY=5.00
PRIMER_PAIR_779_COMPL_END=2.00
PRIMER_PAIR_779_PRODUCT_SIZE=224
PRIMER_PAIR_779_PRODUCT_TM=77.7
PRIMER_PAIR_780_PENALTY=11.864150
PRIMER_LEFT_780_PENALTY=6.610485
PRIMER_RIGHT_780_PENALTY=5.253665
PRIMER_LEFT_780_SEQUENCE=TTGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_780_SEQUENCE=GAAGGGCATCACTTACAAACTCAC
PRIMER_LEFT_780=415,24
PRIMER_RIGHT_780=581,24
PRIMER_LEFT_780_TM=62.610
PRIMER_RIGHT_780_TM=61.254
PRIMER_LEFT_780_GC_PERCENT=45.833
PRIMER_RIGHT_780_GC_PERCENT=45.833
PRIMER_LEFT_780_SELF_ANY=5.00
PRIMER_RIGHT_780_SELF_ANY=4.00
PRIMER_LEFT_780_SELF_END=1.00
PRIMER_RIGHT_780_SELF_END=0.00
PRIMER_LEFT_780_END_STABILITY=6.4000
PRIMER_RIGHT_780_END_STABILITY=6.4000
PRIMER_PAIR_780_COMPL_ANY=5.00
PRIMER_PAIR_780_COMPL_END=1.00
PRIMER_PAIR_780_PRODUCT_SIZE=167
PRIMER_PAIR_780_PRODUCT_TM=77.9
PRIMER_PAIR_781_PENALTY=11.987178
PRIMER_LEFT_781_PENALTY=6.638755
PRIMER_RIGHT_781_PENALTY=5.348422
PRIMER_LEFT_781_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGA
PRIMER_RIGHT_781_SEQUENCE=GCATCACTTACAAACTCACCAACTA
PRIMER_LEFT_781=411,25
PRIMER_RIGHT_781=576,25
PRIMER_LEFT_781_TM=61.639
PRIMER_RIGHT_781_TM=59.652
PRIMER_LEFT_781_GC_PERCENT=44.000
PRIMER_RIGHT_781_GC_PERCENT=40.000
PRIMER_LEFT_781_SELF_ANY=5.00
PRIMER_RIGHT_781_SELF_ANY=2.00
PRIMER_LEFT_781_SELF_END=1.00
PRIMER_RIGHT_781_SELF_END=2.00
PRIMER_LEFT_781_END_STABILITY=8.2000
PRIMER_RIGHT_781_END_STABILITY=5.7000
PRIMER_PAIR_781_COMPL_ANY=4.00
PRIMER_PAIR_781_COMPL_END=0.00
PRIMER_PAIR_781_PRODUCT_SIZE=166
PRIMER_PAIR_781_PRODUCT_TM=77.5
PRIMER_PAIR_782_PENALTY=12.261023
PRIMER_LEFT_782_PENALTY=9.116934
PRIMER_RIGHT_782_PENALTY=3.144089
PRIMER_LEFT_782_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAGTGA
PRIMER_RIGHT_782_SEQUENCE=ACTTTCTTCAGCCAGTGGG
PRIMER_LEFT_782=547,27
PRIMER_RIGHT_782=778,19
PRIMER_LEFT_782_TM=62.117
PRIMER_RIGHT_782_TM=57.856
PRIMER_LEFT_782_GC_PERCENT=40.741
PRIMER_RIGHT_782_GC_PERCENT=52.632
PRIMER_LEFT_782_SELF_ANY=4.00
PRIMER_RIGHT_782_SELF_ANY=5.00
PRIMER_LEFT_782_SELF_END=1.00
PRIMER_RIGHT_782_SELF_END=3.00
PRIMER_LEFT_782_END_STABILITY=6.4000
PRIMER_RIGHT_782_END_STABILITY=9.4000
PRIMER_PAIR_782_COMPL_ANY=4.00
PRIMER_PAIR_782_COMPL_END=0.00
PRIMER_PAIR_782_PRODUCT_SIZE=232
PRIMER_PAIR_782_PRODUCT_TM=77.5
PRIMER_PAIR_783_PENALTY=12.283497
PRIMER_LEFT_783_PENALTY=6.944753
PRIMER_RIGHT_783_PENALTY=5.338743
PRIMER_LEFT_783_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_783_SEQUENCE=AACTCACCAACTAAGCAGCGGTA
PRIMER_LEFT_783=413,25
PRIMER_RIGHT_783=564,23
PRIMER_LEFT_783_TM=61.945
PRIMER_RIGHT_783_TM=62.339
PRIMER_LEFT_783_GC_PERCENT=44.000
PRIMER_RIGHT_783_GC_PERCENT=47.826
PRIMER_LEFT_783_SELF_ANY=5.00
PRIMER_RIGHT_783_SELF_ANY=3.00
PRIMER_LEFT_783_SELF_END=0.00
PRIMER_RIGHT_783_SELF_END=2.00
PRIMER_LEFT_783_END_STABILITY=6.7000
PRIMER_RIGHT_783_END_STABILITY=8.9000
PRIMER_PAIR_783_COMPL_ANY=5.00
PRIMER_PAIR_783_COMPL_END=2.00
PRIMER_PAIR_783_PRODUCT_SIZE=152
PRIMER_PAIR_783_PRODUCT_TM=77.5
PRIMER_PAIR_784_PENALTY=12.308424
PRIMER_LEFT_784_PENALTY=6.944753
PRIMER_RIGHT_784_PENALTY=5.363671
PRIMER_LEFT_784_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_784_SEQUENCE=AGAAGGGCATCACTTACAAACTCA
PRIMER_LEFT_784=412,25
PRIMER_RIGHT_784=582,24
PRIMER_LEFT_784_TM=61.945
PRIMER_RIGHT_784_TM=61.364
PRIMER_LEFT_784_GC_PERCENT=44.000
PRIMER_RIGHT_784_GC_PERCENT=41.667
PRIMER_LEFT_784_SELF_ANY=5.00
PRIMER_RIGHT_784_SELF_ANY=4.00
PRIMER_LEFT_784_SELF_END=0.00
PRIMER_RIGHT_784_SELF_END=1.00
PRIMER_LEFT_784_END_STABILITY=7.0000
PRIMER_RIGHT_784_END_STABILITY=6.4000
PRIMER_PAIR_784_COMPL_ANY=5.00
PRIMER_PAIR_784_COMPL_END=2.00
PRIMER_PAIR_784_PRODUCT_SIZE=171
PRIMER_PAIR_784_PRODUCT_TM=77.7
PRIMER_PAIR_785_PENALTY=12.801856
PRIMER_LEFT_785_PENALTY=7.438185
PRIMER_RIGHT_785_PENALTY=5.363671
PRIMER_LEFT_785_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCTG
PRIMER_RIGHT_785_SEQUENCE=TCTCCTGGTGTAAGAATTTGCACT
PRIMER_LEFT_785=410,25
PRIMER_RIGHT_785=617,24
PRIMER_LEFT_785_TM=62.438
PRIMER_RIGHT_785_TM=61.364
PRIMER_LEFT_785_GC_PERCENT=48.000
PRIMER_RIGHT_785_GC_PERCENT=41.667
PRIMER_LEFT_785_SELF_ANY=5.00
PRIMER_RIGHT_785_SELF_ANY=5.00
PRIMER_LEFT_785_SELF_END=1.00
PRIMER_RIGHT_785_SELF_END=3.00
PRIMER_LEFT_785_END_STABILITY=9.7000
PRIMER_RIGHT_785_END_STABILITY=7.9000
PRIMER_PAIR_785_COMPL_ANY=4.00
PRIMER_PAIR_785_COMPL_END=2.00
PRIMER_PAIR_785_PRODUCT_SIZE=208
PRIMER_PAIR_785_PRODUCT_TM=77.9
PRIMER_PAIR_786_PENALTY=12.993577
PRIMER_LEFT_786_PENALTY=7.562338
PRIMER_RIGHT_786_PENALTY=5.431239
PRIMER_LEFT_786_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTACC
PRIMER_RIGHT_786_SEQUENCE=GTAAGAATTTGCACTTGTCAGGAAC
PRIMER_LEFT_786=407,25
PRIMER_RIGHT_786=608,25
PRIMER_LEFT_786_TM=62.562
PRIMER_RIGHT_786_TM=60.431
PRIMER_LEFT_786_GC_PERCENT=48.000
PRIMER_RIGHT_786_GC_PERCENT=40.000
PRIMER_LEFT_786_SELF_ANY=6.00
PRIMER_RIGHT_786_SELF_ANY=4.00
PRIMER_LEFT_786_SELF_END=1.00
PRIMER_RIGHT_786_SELF_END=1.00
PRIMER_LEFT_786_END_STABILITY=6.9000
PRIMER_RIGHT_786_END_STABILITY=7.9000
PRIMER_PAIR_786_COMPL_ANY=5.00
PRIMER_PAIR_786_COMPL_END=1.00
PRIMER_PAIR_786_PRODUCT_SIZE=202
PRIMER_PAIR_786_PRODUCT_TM=77.8
PRIMER_PAIR_787_PENALTY=13.169124
PRIMER_LEFT_787_PENALTY=7.562338
PRIMER_RIGHT_787_PENALTY=5.606786
PRIMER_LEFT_787_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTAC
PRIMER_RIGHT_787_SEQUENCE=CCTGGTGTAAGAATTTGCACTTGT
PRIMER_LEFT_787=406,25
PRIMER_RIGHT_787=614,24
PRIMER_LEFT_787_TM=62.562
PRIMER_RIGHT_787_TM=61.607
PRIMER_LEFT_787_GC_PERCENT=48.000
PRIMER_RIGHT_787_GC_PERCENT=41.667
PRIMER_LEFT_787_SELF_ANY=6.00
PRIMER_RIGHT_787_SELF_ANY=5.00
PRIMER_LEFT_787_SELF_END=3.00
PRIMER_RIGHT_787_SELF_END=2.00
PRIMER_LEFT_787_END_STABILITY=5.4000
PRIMER_RIGHT_787_END_STABILITY=6.7000
PRIMER_PAIR_787_COMPL_ANY=4.00
PRIMER_PAIR_787_COMPL_END=2.00
PRIMER_PAIR_787_PRODUCT_SIZE=209
PRIMER_PAIR_787_PRODUCT_TM=78.2
PRIMER_PAIR_788_PENALTY=13.427244
PRIMER_LEFT_788_PENALTY=7.723082
PRIMER_RIGHT_788_PENALTY=5.704162
PRIMER_LEFT_788_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_788_SEQUENCE=CTTACAAACTCACCAACTAAGCAGC
PRIMER_LEFT_788=414,25
PRIMER_RIGHT_788=570,25
PRIMER_LEFT_788_TM=62.723
PRIMER_RIGHT_788_TM=60.704
PRIMER_LEFT_788_GC_PERCENT=44.000
PRIMER_RIGHT_788_GC_PERCENT=44.000
PRIMER_LEFT_788_SELF_ANY=5.00
PRIMER_RIGHT_788_SELF_ANY=4.00
PRIMER_LEFT_788_SELF_END=1.00
PRIMER_RIGHT_788_SELF_END=3.00
PRIMER_LEFT_788_END_STABILITY=6.4000
PRIMER_RIGHT_788_END_STABILITY=9.7000
PRIMER_PAIR_788_COMPL_ANY=3.00
PRIMER_PAIR_788_COMPL_END=1.00
PRIMER_PAIR_788_PRODUCT_SIZE=157
PRIMER_PAIR_788_PRODUCT_TM=77.5
PRIMER_PAIR_789_PENALTY=14.268879
PRIMER_LEFT_789_PENALTY=8.555845
PRIMER_RIGHT_789_PENALTY=5.713033
PRIMER_LEFT_789_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAAC
PRIMER_RIGHT_789_SEQUENCE=GAACGAGAAGGGCATCACTTACA
PRIMER_LEFT_789=412,26
PRIMER_RIGHT_789=587,23
PRIMER_LEFT_789_TM=62.556
PRIMER_RIGHT_789_TM=62.713
PRIMER_LEFT_789_GC_PERCENT=46.154
PRIMER_RIGHT_789_GC_PERCENT=47.826
PRIMER_LEFT_789_SELF_ANY=5.00
PRIMER_RIGHT_789_SELF_ANY=4.00
PRIMER_LEFT_789_SELF_END=0.00
PRIMER_RIGHT_789_SELF_END=0.00
PRIMER_LEFT_789_END_STABILITY=6.7000
PRIMER_RIGHT_789_END_STABILITY=6.0000
PRIMER_PAIR_789_COMPL_ANY=5.00
PRIMER_PAIR_789_COMPL_END=1.00
PRIMER_PAIR_789_PRODUCT_SIZE=176
PRIMER_PAIR_789_PRODUCT_TM=77.9
PRIMER_PAIR_790_PENALTY=14.372398
PRIMER_LEFT_790_PENALTY=8.647019
PRIMER_RIGHT_790_PENALTY=5.725379
PRIMER_LEFT_790_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAACT
PRIMER_RIGHT_790_SEQUENCE=AATTTGCACTTGTCAGGAACGAG
PRIMER_LEFT_790=413,26
PRIMER_RIGHT_790=603,23
PRIMER_LEFT_790_TM=62.647
PRIMER_RIGHT_790_TM=62.725
PRIMER_LEFT_790_GC_PERCENT=42.308
PRIMER_RIGHT_790_GC_PERCENT=43.478
PRIMER_LEFT_790_SELF_ANY=5.00
PRIMER_RIGHT_790_SELF_ANY=4.00
PRIMER_LEFT_790_SELF_END=1.00
PRIMER_RIGHT_790_SELF_END=2.00
PRIMER_LEFT_790_END_STABILITY=6.4000
PRIMER_RIGHT_790_END_STABILITY=8.1000
PRIMER_PAIR_790_COMPL_ANY=5.00
PRIMER_PAIR_790_COMPL_END=2.00
PRIMER_PAIR_790_PRODUCT_SIZE=191
PRIMER_PAIR_790_PRODUCT_TM=77.6
PRIMER_PAIR_791_PENALTY=14.372398
PRIMER_LEFT_791_PENALTY=8.647019
PRIMER_RIGHT_791_PENALTY=5.725379
PRIMER_LEFT_791_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGAA
PRIMER_RIGHT_791_SEQUENCE=AGAATTTGCACTTGTCAGGAACG
PRIMER_LEFT_791=411,26
PRIMER_RIGHT_791=605,23
PRIMER_LEFT_791_TM=62.647
PRIMER_RIGHT_791_TM=62.725
PRIMER_LEFT_791_GC_PERCENT=42.308
PRIMER_RIGHT_791_GC_PERCENT=43.478
PRIMER_LEFT_791_SELF_ANY=5.00
PRIMER_RIGHT_791_SELF_ANY=4.00
PRIMER_LEFT_791_SELF_END=0.00
PRIMER_RIGHT_791_SELF_END=2.00
PRIMER_LEFT_791_END_STABILITY=7.0000
PRIMER_RIGHT_791_END_STABILITY=8.4000
PRIMER_PAIR_791_COMPL_ANY=5.00
PRIMER_PAIR_791_COMPL_END=2.00
PRIMER_PAIR_791_PRODUCT_SIZE=195
PRIMER_PAIR_791_PRODUCT_TM=77.6
PRIMER_PAIR_792_PENALTY=3.168124
PRIMER_LEFT_792_PENALTY=2.096340
PRIMER_RIGHT_792_PENALTY=1.071784
PRIMER_LEFT_792_SEQUENCE=ATCAGCTGACTCGCCTGG
PRIMER_RIGHT_792_SEQUENCE=GCCACAGAACATGGCAATC
PRIMER_LEFT_792=2,18
PRIMER_RIGHT_792=316,19
PRIMER_LEFT_792_TM=60.096
PRIMER_RIGHT_792_TM=60.072
PRIMER_LEFT_792_GC_PERCENT=61.111
PRIMER_RIGHT_792_GC_PERCENT=52.632
PRIMER_LEFT_792_SELF_ANY=8.00
PRIMER_RIGHT_792_SELF_ANY=4.00
PRIMER_LEFT_792_SELF_END=3.00
PRIMER_RIGHT_792_SELF_END=2.00
PRIMER_LEFT_792_END_STABILITY=9.7000
PRIMER_RIGHT_792_END_STABILITY=6.9000
PRIMER_PAIR_792_COMPL_ANY=3.00
PRIMER_PAIR_792_COMPL_END=2.00
PRIMER_PAIR_792_PRODUCT_SIZE=315
PRIMER_PAIR_792_PRODUCT_TM=87.4
PRIMER_PAIR_793_PENALTY=4.246200
PRIMER_LEFT_793_PENALTY=2.974589
PRIMER_RIGHT_793_PENALTY=1.271611
PRIMER_LEFT_793_SEQUENCE=GGATCAGCTGACTCGCCT
PRIMER_RIGHT_793_SEQUENCE=GTCTGCCACAGAACATGGC
PRIMER_LEFT_793=0,18
PRIMER_RIGHT_793=320,19
PRIMER_LEFT_793_TM=59.025
PRIMER_RIGHT_793_TM=60.272
PRIMER_LEFT_793_GC_PERCENT=61.111
PRIMER_RIGHT_793_GC_PERCENT=57.895
PRIMER_LEFT_793_SELF_ANY=8.00
PRIMER_RIGHT_793_SELF_ANY=5.00
PRIMER_LEFT_793_SELF_END=3.00
PRIMER_RIGHT_793_SELF_END=2.00
PRIMER_LEFT_793_END_STABILITY=11.4000
PRIMER_RIGHT_793_END_STABILITY=9.6000
PRIMER_PAIR_793_COMPL_ANY=4.00
PRIMER_PAIR_793_COMPL_END=2.00
PRIMER_PAIR_793_PRODUCT_SIZE=321
PRIMER_PAIR_793_PRODUCT_TM=87.4
PRIMER_PAIR_794_PENALTY=4.420324
PRIMER_LEFT_794_PENALTY=3.128205
PRIMER_RIGHT_794_PENALTY=1.292120
PRIMER_LEFT_794_SEQUENCE=GATCAGCTGACTCGCCTGG
PRIMER_RIGHT_794_SEQUENCE=AGTCTGCCACAGAACATGGC
PRIMER_LEFT_794=1,19
PRIMER_RIGHT_794=321,20
PRIMER_LEFT_794_TM=62.128
PRIMER_RIGHT_794_TM=61.292
PRIMER_LEFT_794_GC_PERCENT=63.158
PRIMER_RIGHT_794_GC_PERCENT=55.000
PRIMER_LEFT_794_SELF_ANY=8.00
PRIMER_RIGHT_794_SELF_ANY=5.00
PRIMER_LEFT_794_SELF_END=3.00
PRIMER_RIGHT_794_SELF_END=2.00
PRIMER_LEFT_794_END_STABILITY=9.7000
PRIMER_RIGHT_794_END_STABILITY=9.6000
PRIMER_PAIR_794_COMPL_ANY=5.00
PRIMER_PAIR_794_COMPL_END=2.00
PRIMER_PAIR_794_PRODUCT_SIZE=321
PRIMER_PAIR_794_PRODUCT_TM=87.3
PRIMER_PAIR_795_PENALTY=4.758415
PRIMER_LEFT_795_PENALTY=2.882010
PRIMER_RIGHT_795_PENALTY=1.876405
PRIMER_LEFT_795_SEQUENCE=CTGACTCGCCTGGCTCTG
PRIMER_RIGHT_795_SEQUENCE=ATGGCAATCTGGGGTTCAG
PRIMER_LEFT_795=7,18
PRIMER_RIGHT_795=306,19
PRIMER_LEFT_795_TM=60.882
PRIMER_RIGHT_795_TM=60.876
PRIMER_LEFT_795_GC_PERCENT=66.667
PRIMER_RIGHT_795_GC_PERCENT=52.632
PRIMER_LEFT_795_SELF_ANY=5.00
PRIMER_RIGHT_795_SELF_ANY=4.00
PRIMER_LEFT_795_SELF_END=1.00
PRIMER_RIGHT_795_SELF_END=1.00
PRIMER_LEFT_795_END_STABILITY=6.7000
PRIMER_RIGHT_795_END_STABILITY=7.0000
PRIMER_PAIR_795_COMPL_ANY=4.00
PRIMER_PAIR_795_COMPL_END=3.00
PRIMER_PAIR_795_PRODUCT_SIZE=300
PRIMER_PAIR_795_PRODUCT_TM=87.7
PRIMER_PAIR_796_PENALTY=5.667928
PRIMER_LEFT_796_PENALTY=3.791523
PRIMER_RIGHT_796_PENALTY=1.876405
PRIMER_LEFT_796_SEQUENCE=AGCTGACTCGCCTGGCTCT
PRIMER_RIGHT_796_SEQUENCE=AGAACATGGCAATCTGGGG
PRIMER_LEFT_796=5,19
PRIMER_RIGHT_796=311,19
PRIMER_LEFT_796_TM=62.792
PRIMER_RIGHT_796_TM=60.876
PRIMER_LEFT_796_GC_PERCENT=63.158
PRIMER_RIGHT_796_GC_PERCENT=52.632
PRIMER_LEFT_796_SELF_ANY=5.00
PRIMER_RIGHT_796_SELF_ANY=4.00
PRIMER_LEFT_796_SELF_END=0.00
PRIMER_RIGHT_796_SELF_END=0.00
PRIMER_LEFT_796_END_STABILITY=7.9000
PRIMER_RIGHT_796_END_STABILITY=11.2000
PRIMER_PAIR_796_COMPL_ANY=4.00
PRIMER_PAIR_796_COMPL_END=2.00
PRIMER_PAIR_796_PRODUCT_SIZE=307
PRIMER_PAIR_796_PRODUCT_TM=87.5
PRIMER_PAIR_797_PENALTY=5.924822
PRIMER_LEFT_797_PENALTY=3.873568
PRIMER_RIGHT_797_PENALTY=2.051254
PRIMER_LEFT_797_SEQUENCE=GCTGACTCGCCTGGCTCT
PRIMER_RIGHT_797_SEQUENCE=AACATGGCAATCTGGGGTTC
PRIMER_LEFT_797=6,18
PRIMER_RIGHT_797=309,20
PRIMER_LEFT_797_TM=61.874
PRIMER_RIGHT_797_TM=62.051
PRIMER_LEFT_797_GC_PERCENT=66.667
PRIMER_RIGHT_797_GC_PERCENT=50.000
PRIMER_LEFT_797_SELF_ANY=5.00
PRIMER_RIGHT_797_SELF_ANY=4.00
PRIMER_LEFT_797_SELF_END=0.00
PRIMER_RIGHT_797_SELF_END=0.00
PRIMER_LEFT_797_END_STABILITY=7.9000
PRIMER_RIGHT_797_END_STABILITY=7.9000
PRIMER_PAIR_797_COMPL_ANY=3.00
PRIMER_PAIR_797_COMPL_END=0.00
PRIMER_PAIR_797_PRODUCT_SIZE=304
PRIMER_PAIR_797_PRODUCT_TM=87.6
PRIMER_PAIR_798_PENALTY=5.924822
PRIMER_LEFT_798_PENALTY=3.873568
PRIMER_RIGHT_798_PENALTY=2.051254
PRIMER_LEFT_798_SEQUENCE=AGCTGACTCGCCTGGCTC
PRIMER_RIGHT_798_SEQUENCE=GAACATGGCAATCTGGGGTT
PRIMER_LEFT_798=5,18
PRIMER_RIGHT_798=310,20
PRIMER_LEFT_798_TM=61.874
PRIMER_RIGHT_798_TM=62.051
PRIMER_LEFT_798_GC_PERCENT=66.667
PRIMER_RIGHT_798_GC_PERCENT=50.000
PRIMER_LEFT_798_SELF_ANY=5.00
PRIMER_RIGHT_798_SELF_ANY=4.00
PRIMER_LEFT_798_SELF_END=1.00
PRIMER_RIGHT_798_SELF_END=1.00
PRIMER_LEFT_798_END_STABILITY=9.4000
PRIMER_RIGHT_798_END_STABILITY=9.4000
PRIMER_PAIR_798_COMPL_ANY=3.00
PRIMER_PAIR_798_COMPL_END=1.00
PRIMER_PAIR_798_PRODUCT_SIZE=306
PRIMER_PAIR_798_PRODUCT_TM=87.6
PRIMER_PAIR_799_PENALTY=6.053251
PRIMER_LEFT_799_PENALTY=3.902978
PRIMER_RIGHT_799_PENALTY=2.150273
PRIMER_LEFT_799_SEQUENCE=CTGACTCGCCTGGCTCTGA
PRIMER_RIGHT_799_SEQUENCE=ATGGCAATCTGGGGTTCA
PRIMER_LEFT_799=7,19
PRIMER_RIGHT_799=306,18
PRIMER_LEFT_799_TM=62.903
PRIMER_RIGHT_799_TM=59.850
PRIMER_LEFT_799_GC_PERCENT=63.158
PRIMER_RIGHT_799_GC_PERCENT=50.000
PRIMER_LEFT_799_SELF_ANY=5.00
PRIMER_RIGHT_799_SELF_ANY=4.00
PRIMER_LEFT_799_SELF_END=3.00
PRIMER_RIGHT_799_SELF_END=1.00
PRIMER_LEFT_799_END_STABILITY=6.7000
PRIMER_RIGHT_799_END_STABILITY=6.7000
PRIMER_PAIR_799_COMPL_ANY=3.00
PRIMER_PAIR_799_COMPL_END=3.00
PRIMER_PAIR_799_PRODUCT_SIZE=300
PRIMER_PAIR_799_PRODUCT_TM=87.7
PRIMER_PAIR_800_PENALTY=6.089775
PRIMER_LEFT_800_PENALTY=3.939502
PRIMER_RIGHT_800_PENALTY=2.150273
PRIMER_LEFT_800_SEQUENCE=TGACTCGCCTGGCTCTGA
PRIMER_RIGHT_800_SEQUENCE=CATGGCAATCTGGGGTTC
PRIMER_LEFT_800=8,18
PRIMER_RIGHT_800=307,18
PRIMER_LEFT_800_TM=61.940
PRIMER_RIGHT_800_TM=59.850
PRIMER_LEFT_800_GC_PERCENT=61.111
PRIMER_RIGHT_800_GC_PERCENT=55.556
PRIMER_LEFT_800_SELF_ANY=5.00
PRIMER_RIGHT_800_SELF_ANY=4.00
PRIMER_LEFT_800_SELF_END=3.00
PRIMER_RIGHT_800_SELF_END=0.00
PRIMER_LEFT_800_END_STABILITY=6.7000
PRIMER_RIGHT_800_END_STABILITY=7.9000
PRIMER_PAIR_800_COMPL_ANY=3.00
PRIMER_PAIR_800_COMPL_END=2.00
PRIMER_PAIR_800_PRODUCT_SIZE=300
PRIMER_PAIR_800_PRODUCT_TM=87.7
PRIMER_PAIR_801_PENALTY=7.071717
PRIMER_LEFT_801_PENALTY=4.921444
PRIMER_RIGHT_801_PENALTY=2.150273
PRIMER_LEFT_801_SEQUENCE=CAGCTGACTCGCCTGGCT
PRIMER_RIGHT_801_SEQUENCE=GAACATGGCAATCTGGGG
PRIMER_LEFT_801=4,18
PRIMER_RIGHT_801=310,18
PRIMER_LEFT_801_TM=62.921
PRIMER_RIGHT_801_TM=59.850
PRIMER_LEFT_801_GC_PERCENT=66.667
PRIMER_RIGHT_801_GC_PERCENT=55.556
PRIMER_LEFT_801_SELF_ANY=6.00
PRIMER_RIGHT_801_SELF_ANY=4.00
PRIMER_LEFT_801_SELF_END=3.00
PRIMER_RIGHT_801_SELF_END=0.00
PRIMER_LEFT_801_END_STABILITY=9.7000
PRIMER_RIGHT_801_END_STABILITY=11.2000
PRIMER_PAIR_801_COMPL_ANY=3.00
PRIMER_PAIR_801_COMPL_END=1.00
PRIMER_PAIR_801_PRODUCT_SIZE=307
PRIMER_PAIR_801_PRODUCT_TM=87.6
=
PRIMER_MIN_THREE_PRIME_DISTANCE=-1
SEQUENCE_ID=ok_regions_test4
SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA
PRIMER_NUM_RETURN=200
PRIMER_PRODUCT_SIZE_RANGE=200-200
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MIN_TM=0
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,,
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2260, ok 1
PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2258, ok 3
PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=10.824243
PRIMER_LEFT_0_PENALTY=7.997505
PRIMER_RIGHT_0_PENALTY=2.826738
PRIMER_LEFT_0_SEQUENCE=TAACAATTGAATGTCTGCAC
PRIMER_RIGHT_0_SEQUENCE=GGGGTGACTGTTAAAAGTGC
PRIMER_LEFT_0=238,20
PRIMER_RIGHT_0=437,20
PRIMER_LEFT_0_TM=52.002
PRIMER_RIGHT_0_TM=57.173
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=200
PRIMER_PAIR_0_PRODUCT_TM=75.6
=
SEQUENCE_ID=ok_regions_test5
SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA
PRIMER_NUM_RETURN=200
PRIMER_PRODUCT_SIZE_RANGE=200-200
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MIN_TM=0
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2261, ok 0
PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2261, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=ok_regions_test6
SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA
PRIMER_NUM_RETURN=200
PRIMER_PRODUCT_SIZE_RANGE=200-200
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MIN_TM=0
PRIMER_MAX_TM=100
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,238,20
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_POLY_X=20
PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2258, ok 3
PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2260, ok 1
PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=16.900814
PRIMER_LEFT_0_PENALTY=8.903310
PRIMER_RIGHT_0_PENALTY=7.997505
PRIMER_LEFT_0_SEQUENCE=TTCGTCTGGGGGGTGTGCAC
PRIMER_RIGHT_0_SEQUENCE=GTGCAGACATTCAATTGTTA
PRIMER_LEFT_0=58,20
PRIMER_RIGHT_0=257,20
PRIMER_LEFT_0_TM=68.903
PRIMER_RIGHT_0_TM=52.002
PRIMER_LEFT_0_GC_PERCENT=65.000
PRIMER_RIGHT_0_GC_PERCENT=35.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=200
PRIMER_PAIR_0_PRODUCT_TM=74.5
=
SEQUENCE_ID=ok_regions_test7
SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA
PRIMER_NUM_RETURN=200
PRIMER_PRODUCT_SIZE_RANGE=200-200
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MIN_TM=0
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20;238,20,,
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2260, ok 1
PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2258, ok 3
PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=10.824243
PRIMER_LEFT_0_PENALTY=7.997505
PRIMER_RIGHT_0_PENALTY=2.826738
PRIMER_LEFT_0_SEQUENCE=TAACAATTGAATGTCTGCAC
PRIMER_RIGHT_0_SEQUENCE=GGGGTGACTGTTAAAAGTGC
PRIMER_LEFT_0=238,20
PRIMER_RIGHT_0=437,20
PRIMER_LEFT_0_TM=52.002
PRIMER_RIGHT_0_TM=57.173
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=200
PRIMER_PAIR_0_PRODUCT_TM=75.6
=
SEQUENCE_ID=ok_regions_test8
SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA
PRIMER_NUM_RETURN=200
PRIMER_PRODUCT_SIZE_RANGE=200-200 150-182
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MIN_TM=0
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 2311, not in any ok left region 2310, ok 1
PRIMER_RIGHT_EXPLAIN=considered 2311, not in any ok right region 2310, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=13.190170
PRIMER_LEFT_0_PENALTY=7.997505
PRIMER_RIGHT_0_PENALTY=5.192665
PRIMER_LEFT_0_SEQUENCE=TAACAATTGAATGTCTGCAC
PRIMER_RIGHT_0_SEQUENCE=GCATACCGCCTAAAGATAAA
PRIMER_LEFT_0=238,20
PRIMER_RIGHT_0=419,20
PRIMER_LEFT_0_TM=52.002
PRIMER_RIGHT_0_TM=54.807
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=182
PRIMER_PAIR_0_PRODUCT_TM=75.3
=
