P3_COMMENT=Test error in specifying PRIMER_MAX_SELF_END
SEQUENCE_TEMPLATE=CTCTGCCCACATCATGGGTGAGGCCATGGAGCGCATCTATGGTGGACACCTGTGCTACGGTCCGCCCATTGAAAACGGCTTCTACTACGACATGCATCTGGAGGGCGAGGGTATCTCCACCAACGACTACGGCGCCATGGAGGGTCTG
SEQUENCE_PRIMER=CTACGACATGCATCTGGAGG
PRIMER_MAX_SELF_END=3
PRIMER_EXPLAIN_FLAG=1
PRIMER_TASK=pick_left_only
PRIMER_PICK_ANYWAY=1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.733171
PRIMER_LEFT_0_PROBLEMS= Similary to 3' end of self too high;
PRIMER_LEFT_0_SEQUENCE=CTACGACATGCATCTGGAGG
PRIMER_LEFT_0=84,20
PRIMER_LEFT_0_TM=59.267
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=9.4000
=
