PRIMER_SEQUENCE_ID=anyway_1
PRIMER_SHOW_OLIGO_PROBLEMS=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_SELF_ANY=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_END=8
PRIMER_NUM_RETURN=1
PRIMER_PICK_ANYWAY=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
=
PRIMER_SEQUENCE_ID=high_tm
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any
PRIMER_MAX_SELF_ANY=11
PRIMER_PAIR_MAX_COMPL_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_target
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=20,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_excl_region
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
EXCLUDED_REGION=20,1
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_incl_region
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
INCLUDED_REGION=1,160
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
=
PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_MAX_TM=60
=
PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
=
PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
=
PRIMER_SEQUENCE_ID=internal_too_short
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccat
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
=
PRIMER_SEQUENCE_ID=internal_ok
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
=
PRIMER_SEQUENCE_ID=internal_too_cold
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_INTERNAL_OLIGO_MIN_TM=60
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
=
PRIMER_SEQUENCE_ID=internal_too_cold2
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=12
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_INTERNAL_OLIGO_MIN_TM=60
PRIMER_MAX_TM=100
PRIMER_MAX_GC=100
=
PRIMER_SEQUENCE_ID=mispriming
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_MAX_SELF_END=12
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_INTERNAL_OLIGO_MIN_TM=60
PRIMER_MAX_TM=100
PRIMER_MAX_GC=100
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_MAX_LIBRARY_MISPRIMING=8
=
