---
title: 'SanVar: Validation of single nucleotide variants with Sanger sequencing'
tags:
  - bioinformatics
  - variant calling
  - next generation sequencing
  - SNP
  - Sanger sequencing
  - Validation
authors:
 - name: Andrea Telatin
   orcid: 0000-0001-7619-281X
   affiliation: Gut Microbes and Health Programme, Quadram Institute Bioscience, Norwich (UK)
date: 3 May 2019
bibliography: paper.bib
---

# Summary
Capillary sequencing (commonly referred to as Sanger sequencing) is still routinely 
used to validate single nucleotide variants detected with 
Next Generation Sequencing (NGS) resequencing approaches 
(e.g. human genome resequencing, exome sequencing, targeted resequencing).

SanVar is a tool to allow for high throughput screening of Sanger validated 
variants (from trace files produced by Applied Biosystems capillary sequencers, in .ab1 format) 
checking a variant calling file (in VCF format).

# References
