# SIFT v.2

SIFT version 2: Sequences are chosen by a conservation cutoff [more details]. This differs from the original version of SIFT by how the sequences are chosen. The procedure is described in Genome Research 12: 436-446.
This will take 10-15 min. because we must search your protein sequence against a database to pick the related sequences. You can also submit your protein sequence and related sequences or aligned sequences if you already have them.
Results are deleted after an hour, so please save them!
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Protein sequence
Name of file containing protein query sequence (fasta format).
-or-
Paste in your protein query sequence (fasta format).

Substitutions to be predicted on (can be left blank)
Enter the name of file containing substitutions of interest [format]:
-or-
Enter the substitutions of interest [format]:

Parameters
Select database to search:
Warnings for you predictions? Change this option
Median conservation of sequences
(between 0 and 4.32, we recommend between 2.75 and 3.25)
Remove sequences more than percent identical to query
This cleans out any polymorphic alleles (sequences in the database that are nearly identical to your protein but already containing the substitutions of interest). The contaminating sequences may cause the substitution to be predicted as tolerated.

Questions or comments?
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