Advanced processing

This section will guide you through advanced processing of files in RAW. It includes: pair-distance distribution analysis using GNOM, DIFT and BIFT methods, ambiguity assessment for shape reconstructions, 3D reconstructions with bead models and electron density, aligning 3D reconstructions with PDB files, calculating theoretica profiles from models using CRYSOL and PDB2SAS, SEC-SAXS data deconvolution using singular value decomposition (SVD) and evolving factor analysis (EFA), baseline corrections for SEC-SAXS data, and regularized alternating least squares (REGALS) of SEC-SAXS and other SAXS data. It refers to the RAW tutorial data.

Select a section below to view the tutorial, or use the next and back buttons at the bottom of the page to navigate through it in order.

• Pair-distance distribution analysis – GNOM in RAW
• Pair-distance distribution analysis – DIFT (DENSS IFT) in RAW
• Pair-distance distribution analysis – BIFT in RAW
• Assessing ambiguity of 3D shape information - AMBIMETER in RAW
• 3D reconstruction with bead models – DAMMIF/N and DAMAVER in RAW
• 3D reconstruction with electron density – DENSS in RAW
• Aligning reconstructions with high resolution shapes
  • Bead models - CIFSUP
  • Electron density
• Theoretical profiles and fitting from models – CRYSOL in RAW
• Theoretical profiles and fitting from models – PDB2SAS (DENSS) in RAW
• Advanced SEC-SAXS processing – Singular value decomposition (SVD)
• Advanced SEC-SAXS processing – Evolving factor analysis (EFA)
• Advanced Series processing – Regularized Alternating Least Squares (REGALS)
  • Deconvolving ion exchange chromatograph coupled SAXS (IEC-SAXS) data
  • Deconvolving equilibrium titration series SAXS data
  • General notes
• Advanced SEC-SAXS processing – Baseline correction
  • Linear Baseline Correction
  • Integral Baseline Correction