Construct small genomes based on the Illumina MiSeq data.
This tool runs A5-miseq pipeline that is designed for assembling DNA sequence data generated on the Illumina sequencing platform. There are many situations where A5-miseq is not the right tool for the job. In order to produce accurate results, A5-miseq requires Illumina data with certain characteristics. A5-miseq will likely not work well with Illumina reads shorter than around 80nt, or reads where the base qualities are low in all or most reads before 60nt. A5-miseq assumes it is assembling homozygous haploid genomes. Use a different assembler for metagenomes and heterozygous diploid or polyploid organisms. Use a different assembler if a tool like FastQC reports your data quality is dubious.
The tool needs a pair of fastq formatted paired-end reads files.
For more details, please check the home page of A5-miseq pipeline and the PLOS One article about the A5 miseq pipeline.
Output is a PNG, SVG or PDF formatted image file.