ACCEPTABLE LEFT PRIMERS
                                  0-based     #               self  self  qual-
   # sequence                       start ln  N   GC%     Tm   any   end   lity
   0 TGACNACTGACGATGCAGAC              15 20  1 52.63 58.681  4.00  1.00  3.319
   1 ATGACNACTGACGATGCAGAC             14 21  1 50.00 59.059  4.00  1.00  3.941
   2 TGACNACTGACGATGCAGA               15 19  1 50.00 57.619  4.00  0.00  3.381
   3 ATGACNACTGACGATGCAGA              14 20  1 47.37 58.080  4.00  0.00  3.920
