Dear Editor

You will find attached a manuscript reporting an original method of spot detection on 2D gels. This method is sensitive and allows for accurate detection of faint and overlapping spots. The manuscript describes the different steps of image processing, and it presents the program, which is freely available and open source. The manuscript was written in the format of a technical brief. At first it was prepared for the special issue for Angelika Gorg. I guess it is too late now for this special issue, but I hope the reviewers will find it interesting, and will consider that it deserves publication in Proteomics.
Thank you for considering our manuscript.

As requested, I certify that both authors have seen this version of the paper and agree with its publication.

Thank you in advance for handling this manuscript,

Best regards

Michel Zivy

